Means and methods of predicting response to treatment of hepatitis b

FIELD: biotechnology.

SUBSTANCE: invention relates to a method of determining whether a patient with hepatitis b (HBV) has an increased probability of a positive outcome of hepatitis B treatment compared to the average probability in a population of patients with hepatitis B. The proposed method includes establishing whether the sample with nucleic acid of specified patient with hepatitis B, associated with positive result of G allele of SNP rs 12356193.

EFFECT: invention allows you to determine whether a patient with HBV will respond to HBV therapy prior to therapy, and gives the possibility to avoid unnecessary expenditure and the emergence of side-effects and complications during treatment.

9 cl, 24 dwg, 3 tbl, 4 ex



Same patents:

FIELD: chemistry.

SUBSTANCE: invention relates to investigation and analysis of high-molecular weight materials via infrared spectroscopy when determining the composition of polyacrylate and polyacrylonitrile copolymers to control the quality of carbon fibre. The method includes measuring the infrared absorption spectrum of films of test samples of polyacrylonitrile fibre using Fourier transform infrared spectroscopy in the 3000-800 cm-1 region and determining the content of acrylonitrile and methyl acrylate from normalised spectra based on characteristic peaks thereof. During sample preparation, the number of sample preparation steps is maximally simplified and reduced and dimethyl sulphoxide which does not absorb in the operating infrared region is used. When processing the obtained infrared spectra, the method employs adjustment of the entire base line, smoothing of the shape of the peaks of the investigated compounds and decomposition of the composite peak at 1733±3 cm-1 into components.

EFFECT: invention provides a method for reproducible, precision and sensitive determination of basic components of polyacrylonitrile.

2 cl, 3 tbl, 1 dwg

FIELD: chemistry.

SUBSTANCE: invention relates to genetic engineering and biotechnology. Disclosed is a method of evaluating bioactivity of chemical compounds, where the first step includes transient transfection of cell line HEK 293 with plasmid vector pX-Y-neo (X is any eukaryote transcription factor, Y is a proteotypic peptide corresponding to said transcription factor), which contains a minimal human adenovirus type 5 promoter; a green fluorescent protein gene; a nucleotide sequence which codes the binding site of the transcription factor; a nucleotide sequence which codes the proteotypic peptide; a neomycin resistance gene; the second step includes determining the activity of the transcription factor via fluorescent analysis and chromatographic-mass spectrometer measurement of the content of the proteotypic peptide in the transfected cell culture in the presence of the test substance compared to a transfected intact cell culture.

EFFECT: invention provides fast and highly sensitive evaluation of bioactivity of chemical compounds.

2 dwg, 1 ex

FIELD: agriculture.

SUBSTANCE: device for research of physical-mechanical properties of tuberous roots comprises a frame (1) with an electric motor (2) attached to it, on a shaft of which a removable disc (3) is mounted with the surface under study, and the guide (4) on which a movable trolley (5) is mounted. The movable trolley (5) is connected on one side to the screw mechanism (7) through the spring (6), and on the other side to a load (8) through the block (9). The device is provided with a frequency converter (13) which enables to adjust smoothly the speed of rotation of the removable disc (3), and also a screw mechanism (15) with a guide by which the gap between the trolley (5) and the removable disc (3) is implemented.

EFFECT: increase in the accuracy of the results of research on friction process of rest and movement of tuberous roots on different surfaces.

1 dwg

FIELD: measurement equipment.

SUBSTANCE: invention relates to the field of assessment of atmospheric air pollution degree and may be used in monitoring of atmospheric air in background and urbanised territory. The method provides for identification of a test site with size of 25×25 m, detection of external criteria of lichen on the test site, detection of available indicator types of lichen and frequency of their occurrence. On the basis of the produced data the lichenoindex is calculated, according to the provided classification of lichenoindex, they determine degree of atmospheric air pollution.

EFFECT: invention makes it possible to detect degree of atmospheric air pollution by lichen.

4 tbl, 1 ex

FIELD: biotechnologies.

SUBSTANCE: object is positioned on porous substrate, fixed to the substrate surface and scanned by probe microscopy method. Substrate with through pores of smaller size than the diameter of a study object is used, and an object is fixated by laminar flow of liquid or gas supplied to the substrate from the side of scanning, with clamping force exerted by the flow on an object within 10-12-10-3 N range.

EFFECT: possible study of structures and mechanical properties of organic and inorganic objects, enhanced information content of nano and micro object studies by probe microscopy.

7 ex

FIELD: agriculture.

SUBSTANCE: invention relates to ecology. The invention provides a method of determining the quality of the environment by EPR-spectroscopy of lichens, including the collection of samples of lichen thalli from the trunks of trees growing in an industrial and background area, which is not contaminated by anthropogenic emissions into the environment, cleaning, drying, grinding, which is characterised by the fact, that drying is carried out at a temperature of 85-95°C to constant weight and ground, the EPR spectra are removed, from which the concentration of paramagnetic centres is determined, in the excess of the concentration of the paramagnetic centres in the lichen samples collected in the industrial zone, over the concentration of the paramagnetic centres of the lichen samples from the background area the low quality of the environment in the industrial zone is detected, and in case of equal concentrations of the paramagnetic centres - the acceptable quality of the environment is detected, and in the studies the samples of the same species of lichen are used.

EFFECT: invention provides the improvement of the method of lichenoindication, improvement of the quality of evaluation of the test objects, obtaining objective result.

2 ex, 2 tbl, 3 dwg

FIELD: ecology.

SUBSTANCE: method of determining ammonium compounds in the atmosphere of livestock complexes comprises collecting samples of lichen from trees growing in the background zone, which has no emissions of pollutants into the atmosphere. The data for the samples of lichen collected in the zone of pollutants emission to the atmosphere is compared with data for laboratory standards by IR spectroscopy method. For obtaining the standards under laboratory conditions the interaction process of lichen of the background zone with emission of pollutants contributing to formation of ammonium sulphate is simulated. Lichen Parmelia sulcata is used as bioindicator.

EFFECT: invention enables to determine the level of ammonium compounds in the atmosphere of livestock complexes.

2 tbl, 1 dwg

FIELD: metallurgy.

SUBSTANCE: invention relates to ferrous metallurgy and can be used to determine chemical composition of materials containing lump metal and used as raw material in cast iron production. The method involves separation of material into metal and slag fractions, measurement of metal fraction weight, grinding of slag fraction down to the fineness of 5 mm at most and determination of weight ratio of total ferrum and of necessary components in it by complete acid digestion, calculation of weight ratio of total ferrum and of components in the material, after grinding a sample is taken with the fineness of from 0.16 mm to 5 mm at most and chemical analysis is performed.

EFFECT: improved information value and reliability of analysis.

FIELD: medicine.

SUBSTANCE: invention refers to medicine and can be used for the prediction of the early stage of lymphocyte apoptosis. That is ensured by isolating cells, incubating for 48 hours at temperature 37°C with 5% CO2 with adding apoptosis inductor, dexamethasone in the concentration of 10-4 mole/ml. A lymphocyte viability is quantified by trypan blue inclusion, the recovered and oxidised glutathione concentrations are measured in lymphocyte lysate after the 30-minute pre-incubation with 2-vinylpyridine 10 mM. The early stage of lymphocyte apoptosis is stated, if observing an integrated decrease of the recovered glutathione concentration by 17% and more and an increase of the oxidised glutathione concentration by 19% and more as compared to the reference.

EFFECT: using the presented method in medical practice enables predicting the antioxidant state of the patient's body accompanying various diseases as shown by the early stage of lymphocyte apoptosis evaluated.

1 tbl

FIELD: chemistry.

SUBSTANCE: method includes the selection and preparation of samples to be analysed, selection of specified volumes of solutions of a test system components, placement of the samples to be analysed and the test system components into a cuvette, registration of chemiluminiscence with further quantitative estimation of its value with taking into account the background signal of chemiluminiscence. The weight of porting of the sample of the material to be analysed is taken such that corresponds to the value of a specific surface 0.20±0.05 m2/g, and in case when it is not possible to determine the value of the specific surface of the sample to be tested, the weight of the taken portion is 0.010±0.005 g. The portion of the sample of the material to be analysed is placed into a cuvette with the further successive addition of the test system components: 0.01M solution of luminal in 0.5 NaOH solution and a solution of hydrogen peroxide of a 20-30% concentration to fill the working space in the cuvette, keeping the ratio luminal:hydrogen peroxide equal to 2:5. After that, values of chemiluminiscence are registered for 125 minutes and the total value of chemiluminiscence is calculated.

EFFECT: identification of the free-radical activity of solid materials by the method of the chemiluminiscence registration by means of the system of chemical reagents without the application of biological substrates in the test system.

3 tbl, 3 ex

FIELD: chemistry.

SUBSTANCE: invention relates to field of biotechnology, immunology and genetic engineering. Genetic modification contains deletion of endogenic low-affinity FcγR locus, where contain functional FcRγ-chain. Modified mice express up to five genes of low-affinity human FcγR on antigen-presenting cells of host immune system. Also described is method of screening substance, containing Fc site of human antibody by means of said mice. Invention can be used in the field of medicine.

EFFECT: presented are genetically modified mice, not related to man, and methods and compositions for their obtaining and application.

15 cl, 11 dwg, 6 tbl, 8 ex

FIELD: measurement equipment.

SUBSTANCE: group of inventions relates to the incubation of water samples. The incubator for water samples and the method for incubation of water samples is offered. The incubator is designed as an association of light- and heat-isolated cells. Each cell comprises a cover, a housing, a barrel with heat-isolated from the housing, a cell control unit, individual temperature stabilisation system and individual illumination stabilisation system. The individual illumination stabilisation system contains the light-emitting diode in the lower part of the barrel. The light-emitting diode provides the necessary level of light exposure of the sample. The incubation method is implemented in the offered incubator, temperature and constant illumination conditions are pre-set individually for each sample.

EFFECT: inventions allow to perform incubation of water samples in individual conditions of each sample.

4 cl, 2 dwg

FIELD: measurement equipment.

SUBSTANCE: group of inventions relates to the field of biochemistry. A biosensor system and test sensors (versions) are proposed to determine concentration of an analysed substance in a sample. The biosensor system includes a test sensor, comprising at least two electric conductors, a reaction agent containing a binding substance, including at least one water soluble polymer material, buffer salt, a water soluble medium for transfer of one or two electrons, containing at least 20% (wt/wt) of inorganic salt of non-transition metal, a fermentative system and a non-ionic surfactant. The biosensor system also comprises a measurement facility to measure speed of oxidation-restoration reaction of the analysed substance.

EFFECT: proposed group provides for accurate determination of dependence of an output signal of a test sensor, which contains compositions of reagent with low total concentration of salt, from concentration of analysed substance in samples of whole blood in a wide range of levels of hematocrit within the limits of not more than 7 seconds.

49 cl, 9 dwg, 7 tbl

FIELD: medicine.

SUBSTANCE: invention aims at a method for identifying microorganisms from a test blood culture sample. The method provides preparing a test sample, conducting a selective lysis and dissolving the cells other than microorganisms of the test sample, laminating the prepared lysate on a density buffer in a hermetically sealed container and centrifuging. The density buffer has a uniform density from approximately 1.025 g/ml to approximately 1.120 g/ml. The microorganisms passing through the above buffer are precipitated on a container bottom. The precipitate is analysed with the use of Raman spectroscopy that enables identifying a genus or a species of the microorganism.

EFFECT: invention enables identifying the microorganisms from the clinical samples over less than 120 min.

13 cl, 4 dwg, 3 ex

FIELD: medicine.

SUBSTANCE: invention relates to the field of biotechnology and cell technology. The claimed invention is aimed at the creation of pluripotent, multipotent and/or self-renewing cells, which are able to start differentiating in a culture into various types of cells and are capable of further differentiation in vivo. The claimed invention is also aimed at the creation of populations of the required differentiating cells, which can be transplanted to patients, genetic modification of endogenic cells and treatment of patients, suffering from diseases, intensity of which can be reduced by means of the said methods.

EFFECT: invention also claims methods of prevention, treatment or retardation of a disease, associated with an infection of immunodeficiency virus.

17 cl, 1 dwg, 13 ex

FIELD: biotechnology.

SUBSTANCE: biological sensor is described, as well as the method of creation of a biological sensor using thin films based on grapheme, graphene oxide or single-walled or multi-walled carbon nanotubes. The biological sensor comprises a substrate, a metal film on the surface of which the intermediate bonding layer is applied, made of a thin film of graphene or a thin film of graphene oxide or a thin film of carbon nanotubes. On the surface of the intermediate bonding layer the biospecific layer is adsorbed conformally and uniformly. The biospecific layer can be used as a layer of molecules of the binding partner of the analyte or a layer of a complex of biological molecules capable of interacting chemically with the molecules of the binding partner and forming a complex with them. Also the biospecific layer can be used as the hydrogel layer, on which the binding partner molecule and/or complex of binding partner molecules and biological molecules is deposited, which can form a chemical bond with the binding partner molecules. The described method of obtaining the biological sensor comprises the stages of applying a metal film, an intermediate bonding layer and a biospecific layer.

EFFECT: high sensitivity of the biosensor in combination with high biospecificity, expanding the range of application of the device, protection of the metallic film from the effects of the environment, the ability to detect large biological objects.

27 cl, 9 dwg

FIELD: chemistry.

SUBSTANCE: claimed invention relates to field of biophysics. Claimed are methods of determining space-time distribution of proteolytic enzyme activity in heterogeneous system, in accordance to which: provided is system in vitro, which contains sample of blood plasma, whole blood, water, lymph, colloidal solution, crystalloid solution or gel, and proteolytic enzyme or its precursor; fluorogenic, chromogenic or luminescent substrate for said enzyme id added; space distribution of signal of released substrate label is registered at specified time moments and space-time distribution of proteolytic enzyme activity is obtained by solving reverse problem of type "reaction-diffusion-convection" taking into account label binding with medium components. Also described is device for realisation of methods in accordance with claimed invention and method of diagnosing hemostasis disorders, based on their application.

EFFECT: invention can be further applied in the study of blood coagulation system and diagnostics of diseases associated with blood coagulation disorders.

22 cl, 6 dwg

FIELD: biotechnologies.

SUBSTANCE: invention relates to the field of gene engineering, specifically, to development of test systems based on fluorescent probes, which may be used as substrates for phospholipases A1 and A2. The probe with the name 1-[3-(2,4-dinitrophenylamino)propanoyl]-2-[8-(9-anthryl)-7E-octenoyl)]-sn-glycero-3-phosphocholine represents an analogue of phosphatidyl choline witth modified fatty acid chains, one of which carries the fluorescent group, and the other one - grouping-suppressor of fluorescence. The fluophor-suppressor pair is: 9-anthryl vinyl - 2,4-dinitrophenyl. The produced probe is included into the test system to determine activity of A2 phospholipase, group IIA, which also includes a micellar matrix, a buffer solution, diglyme and A2 phospholipase of apitoxin as standard.

EFFECT: invention makes it possible to determine activity of A2 phospholipase in blood serum in case of acute pathological processes, in tissue and cell extracts.

2 cl, 3 dwg, 5 ex

FIELD: biotechnologies.

SUBSTANCE: phospholipide fluorescent probe characterised by the following name 1-[13-(4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacen-8-yl)tridecanoyl]-2-(10-{[(2-hydroxynaphthyl-1)azophenyl-4]azophenyl-4}decanoyl)-sn-glycero-3-phosphocholine, is used in the test system to determine activity of A2 phospholipase, group IIA (secFLA2(IIA)) in blood serum, which also contains vesicular and phospholipide matrix for inclusion of a probe, containing phosphatidyl choline, lisophosphatidyl choline and phosphatidyl glycerine, a buffer solution and A2 phospholipase of apitoxin as standard.

EFFECT: invention makes it possible to reliably detect activity of secFLA2 in human blood serum in clinical conditions.

2 cl, 5 dwg, 5 ex

FIELD: medicine.

SUBSTANCE: enzyme immunoassay system for determining a probable time of the introduction of type 1 human immunodeficiency virus (HIV-1) infection, including group O HIV-1 in human serum (plasma). The system contains an immunosorbent of type 1 human immunodeficiency virus antigens (env HIV-1 and group O HIV-1), an assay diluent (PPC) and detecting reagents (conjugates, chromogen/substrate). The invention refers to a method for determining the probable time of the introduction of type 1 human immunodeficiency virus (HIV-1) infection, including group O HIV-1 in human serum (plasma) by analysing patient's blood serum with using the above enzyme immunoassay system.

EFFECT: invention enables the fast and simple determination of the probable time of the introduction of the HIV infection.

2 cl, 4 tbl, 2 ex

FIELD: chemistry.

SUBSTANCE: method includes treating a sample with a denaturing buffer solution, sorbing short RNA on a fibre glass sorbent in the presence of a chaotropic agent, followed by washing off non-bonded biopolymers and chemical reagents from the sorbent and eluting the end product. The biological fluid sample undergoes two-step denaturing, wherein at the first step two volumes of the denaturing buffer solution are added to the sample and at the second step two volumes of 96% ethanol and equal volume of chloroform are added to the obtained mixture, followed by stirring and separating the residue by centrifuging. Non-bonded biopolymers are washed off from the sorbent twice with the buffer solution and elution of the end product from the sorbent is carried out with the buffer solution.

EFFECT: simple method, short duration of the method and high output of short RNA.

3 cl, 3 tbl, 4 ex