Method of selection of selection material of rape (brassica napus l) on feature of resistance to cracking of pods

FIELD: agriculture.

SUBSTANCE: invention relates to the field of agriculture, in particular, to plant breeding. The method includes selection of samples more resistant to cracking of pods by analysing the height of the dense partition in the area of gynophore the phase of green pod. Then, at selected plants with greater height the formed pods and blooming flowers are picked off, crossing is carried out and self-fertilisation on lateral shoots.

EFFECT: method enables to improve the efficiency of the selection process in the selection of breeding material of rape.

1 dwg, 1 tbl, 1 ex

 

The invention relates to agriculture, in particular to the selection and can find application in the selection of genotypes with high resistance to cracking of pods.

Known methods of determining rastreskivaetsja pods: visual assessment of the plants on this topic in the field for several years; in the laboratory by creating conditions similar to the natural; in the use of mechanisms such as lever (pendulum) (G. Kadkol. Brassica shatter-resistance research update // 16thAustralian Research Assembly on Brassicas. Ballarat Victoria 2009).

The closest known adopted for the prototype, is a method for determining the resistance to cracking of pods on indirect indicators on the basis of a number of anatomical and morphological characteristics [Child R.D. Increased resistance to pod shatter in associated with changes in the vascular structure in pods of a resynthesized Brassica napus line // R.D. Child, J.E. Summers, J. Babij, J.W. Farrent, D.M. Bruce // J. of experimental Botany, 2003. - Vol.54, No. 389. - P.1919-1930]. However, with this method the analysis is carried out using a range of indicators, which requires long-term work with the microscope.

The objective of the invention is to increase the efficiency of the selection process in the selection of breeding material of rape on the basis of the resistance to cracking of pods.

To achieve the above objectives, the proposed method, including the sampling resistance to restrictive the July pods on the height of the dense structure of partitions in the field of gynophore in phase green pod on the center of the brush, including tearing formed pods and blooming flowers and holding crossing and selfing on lateral shoots. The combination of added characteristics of the prior art is not revealed. Thus, the claimed method meets the criterion of "novelty". The selection and crosses in one growing season leads to the acceleration of the breeding process.

The basis of the proposed method based on the ability to differentiate genotypes for resistance to cracking the beans in phase green pod and stretched flowering canola.

The proposed method is as follows: in splitting the population in the phase of green beans on the Central brush carried out the measurement of the height of the dense structure of partitions in the field of gynophore. The selected plants with the desired characteristics conduct cross-pollination or self-pollination, depending on the selected scheme selection process.

theoretical basis of the proposed method is a proof of the possibility of determining the resistance to cracking of pods in phase green pod height dense structure of partitions in the field of gynophore followed by pollination on lateral branches.

An example of a specific implementation of the method.

When comparing resistant Rastrelli aniu pods varieties Century napus 1197 with susceptible cultivar Hja 82685 revealed significant differences in the height of the dense structure of partitions in the field of gonopore (Fig.).

Figure the arrow shows the measured area of the walls of the pod in the field of gynophore.

The varieties more resistant to cracking of pods, the height of the dense structure of partitions in the field of gynophore more (see table).

Table
Anatomical and morphological features in the structure of the pods spring rape varieties, distinguished by the degree of rastreskivaetsja.
Considered a sign ofC. napus 1197Hja 82685
The height of the dense structure of partitions in the field of gynophore1,5±0,0820,97±0,099

NDS05=0,144

Thus, the results show the ability to conduct sampling for resistance to rastreskivaetsja pods at the stage of green pod height dense structure of partitions in the field of gynophore with subsequent pollination on lateral branches.

Using the proposed method for the selection of breeding material of rapeseed (Brassica napus L.) by the sign of the resistance to cracking of pods at the stage of green pod will improve the efficiency of the selection process and reduce the time of creation of breeding material with improved characteristics.

The invention can be used in the selection process without preconditions.

The way of selection of breeding material of rapeseed (Brassica napus L.) on the basis of the resistance to cracking of pods, including sampling more resistant to cracking of pods, characterized in that the phase of the green pod analyze the height of the thick walls in the area of gynophore and selected plants with greater height of cut formed pods and blossoming flowers, conduct of crossing and selfing on lateral shoots.



 

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