Method of selection of parent pairs in sheep production |
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IPC classes for russian patent Method of selection of parent pairs in sheep production (RU 2525132):
       
 Method of stimulating embryogenesis of laying hens / 2520092
Method comprises a single treatment of hatching eggs before laying into the incubator from sprayer with the aerosol of 0.01% sodium salt solution of lipoic acid, which is preliminary dissolved and mixed in distilled water at a temperature of 18-22°C. The solution is applied to the surface of egg shells 3-4 hours prior to their incubation.
 Regulation of productive characteristics in birds / 2518681
Invention relates to field of biotechnology. Method includes introduction of RNA molecule into a bird egg. Introduced RNA molecule contains double-stranded region and results in reduction of the level of molecule of RNA and/or protein, included into determination of sex in birds, in the egg. Invention can be used in poultry breeding.
Method of increasing meat productivity of pigs by selection using genetic marker / 2514634
Invention relates to the field of agriculture. The method comprises selection of pigs using a genetic marker. At that the selection and choosing of rearing stock is carried out at achievement of live weight of 90-100 kg in the degree of concentration of free lysine in the muscle tissue of longissimus muscle of back (Musculus Longissimus Dorsi). The animals with the level of lysine concentration of 1.36 mmol/kg of fresh weight and more are left in a breed.
Method of increasing adaptive status of bee family / 2511304
Invention relates to livestock husbandry, in particular, to beekeeping for increasing the adaptive status of the bee family. The microdoses of biologically active substance are administered in the body of bees with water or an aqueous solution of carbohydrates. Dihydroquercetin is used as the biologically active substance.
Method for assessing stress resistance in pigs / 2510852
Invention refers to veterinary science and agriculture. The method provides pig's blood analysis for the following values: malondialdehyde MDA, antioxidant activity AOA, total oxidative activity of blood plasma TOA, catalase activity CT, superoxide dismutase SOD. The two-month pigs having MDA 6.02±0.13 absorption unit/ml and less, AOA 60.86±0.31% and more, TOA 71.64±0.16% and less, CT 57.30±1.05 absorption unit/ml min and more, SOD 14.57±0.44 absorption unit/ml and more are considered to be stress-resistant PSS "-"; the values of MDA 7.63±0.07 absorption unit/ml and more, AOA 50.22±0.42% and less, TOA 79.21±0.83% and more, CT 47.16±1.08 absorption unit/ml min and less, SOD 10.49±0.49 absorption unit/ml and less enables stating the pigs as stress-sensitive PSS "+". The six-month pigs having MDA 7.37±0.17 absorption unit/ml and less, AOA 61.93±0.25% and more, TOA 41.86±0.19% and less, CT 76.68±0.74 absorption unit/ml min and more, SOD 22.80±0.68 absorption unit/ml and more are considered to be stress-resistant PSS "-"; the values of MDA 8.05±0.09 absorption unit/ml and more, AOA 59.72±0.17% and less, TOA 46.21±0.09% and more, CT 71.76±0.51 absorption unit/ml min and less, SOD 19.91±0.59 absorption unit/ml and less enables stating the pigs as stress-sensitive PSS "+".
 Method of obtaining valid molecular-genetic model for pre-clinical tests of novel anti-epileptic medications / 2502257
Invention relates to field of medicine, neurobiology and pharmacokinetics and deals with method of obtaining valid molecular-genetic model of human absence epilepsy. Claimed method consists in the following: parent individuals P with genotype A1/A1 of gene DRD2 are identified by means of genotyping of Taq 1A DRD2 locus in rats of WAG/Rij line, crossed with each other with obtaining offspring F1, which is grown to reproductive age, after that, nonaudiogenic individuals are identified among offspring F1, after which nonaudiogenic individuals of offspring F1 are crossed with each other to obtain offspring F2, which is then also grown to reproductive age with the following selection of nonaudiogenic individuals among them, crossing and selection being performed repeatedly to obtain homogeneous population of nonaudiogenic rats of WAG/Rij line with genotype A1/A1 of gene DRD2, control of "ПВР" type in selected individuals of offspring F1 for further crossing is carried out by means of encephalographic analysis, which includes morphological control.
Method of selection of cattle in kalmyk breed according to meat productivity / 2498569
Invention relates to the field of genetics and animal breeding. The method of selection of cattle of Kalmyk breed is that at the age of 6-month the presence in blood of erythrocytic antigens-markers of tall-growing type: G2 E'3 R2 are detected. In the presence in the genotype of animals of markers of productivity the selection of animals is carried out.
 Young minks performance increase method / 2497371
Invention relates to fur farming field, in particular, to young minks performance increase. The fur animals growing method envisages introduction of biologically active substances (physiological promoters) into the ration of young minks intended for pelt obtainment. The biologically active substances (physiological promoters) are represented by L-carnitine preparation and are introduced in an amount of 30 mg per 1 kg of live weight into the ration in seven days' courses with seven days' intervals between them, once a day in the period of active growth - from June to August inclusive.
 Method of evaluation of broiler chickens / 2496315
Invention relates to the field of agriculture, namely, to selection, and can be used in breeding in poultry pedigree farms. Evaluation of broiler chickens is carried out according to the developed scale for fast and slow-fledging lines of broiler strains; the day-old chickens are distributed into three groups: 6-18 hours - late; 19-32 hours - average; 33 and above - early. The calculation of the duration of the embryonic development from laying to hatching chickens is carried out: the time is recorded from the start of incubation till evaluation of the chickens on development of fledging (T1), the age of chickens is subtracted from it, which is set according to a feather (T2), using the formula: T=T1-T2.
 Method of assessment of velvet antler yielding capacity of siberian red deers / 2491814
Invention relates to a velvet antler reindeer breeding and can be used to identify the productive qualities of Siberian red deers. The method is characterised in the fact that assessment is carried out according to the level of testosterone and/or estradiol secretion in blood serum in its sampling in any month from December to August inclusively, and Siberian red deers should be considered as highly productive with the following levels of secretion of testosterone in blood serum, nmol/l, and time period of its sampling: 1.67 - January, and/or 7.15 - March, and/or 5.41 - April, and/or 1.27 - June and/or 5.13 - August, not less and/or according to the level of estradiol secretion in blood serum, nmol/l, and time period of its sampling: 2.32 - December, and/or 2.91 - January, and/or 1.06 - March, and/or 1.50 - April, and/or 2.41 - June, and/or 2.68 - July, no more.
 Method for detecting glycogen in extract out of organs and tissues in bees / 2256320
The present innovation deals with boiling an extract, cooling, centrifuging, dissolving a residue, cooling, centrifuging, dissolving a residue, adding sulfuric acid into a tube and 1%-condensate's solution followed by heating, cooling, photometry against the control at wave length being 315 nm, as a condensate one should apply resorcinol.
 Method for separating of synanthropic flies from substrate / 2290795
Method involves placing processed substrate with synanthropic fly larvae living thereon into reservoir by means of belt conveyor; setting velocity of belt conveyor so as to provide migration of larvae from substrate thickness into reservoir, onto its surface, said velocity depending on thickness of substrate layer: the greater is thickness of substrate layer , the lower is the velocity, and larvae concentration in upper part of reservoir being provided by making width of reservoir lower part exceeding width of its upper part; upon filling of reservoir, stopping feeding of substrate and removing larvae from upper part of reservoir by means of conveyor equipped with rectangular plates; removing substrate remained in reservoir by means of screw-type conveyor.
Method for producing of bird's gametes / 2294099
Method involves obtaining embryo stem cells from donor-kind birds and introducing stem cells in-ovo to recipient-kind birds, with recipient-kind birds differing from donor-kind birds; incubating recipient-kind cells to pecking stage; growing to sexual maturity phase and collecting gametes of donor-kind birds from recipient-kind birds.
Method for treatment of meat-type chicken eggs / 2308830
Method involves treating eggs with biologically active compositions, with organosilicon substances such as cresacin and meval being used as biologically active compositions. Said compositions are applied in the form of mixture of their solutions onto eggshell 6-12 hours before incubation.
Method for obtaining color-balanced karakul / 2310326
The present innovation deals with raising colored Karakul sheep and could be applied for obtaining qualitative, color-balanced, export-orientated karakul. It is necessary to select stud rams (lambs) aged 1-2 d to detect the peculiarities of distribution, the quantity and quantitative content of the pigment - melanin in hair. Additionally, it is important to carry out iridoscopy for the lambs aged 5-mo along with detection of color purity - the absence of stripes, lines, grooves, lacunes, spots and homogeneity - the absence of contrast distinctions in pigmentation of iris color. Then comes the selection of stud rams at steady distribution of pigment without any variations of color tone along the whole surface of iris and along its all projectional areas of the right and left eyes in 12.00-11.59 sector clockwise. Artificial insemination of females of the same color from these stud rams has been suggested followed by the analysis of the data obtained. The innovation enables to shorten the terms and cheapen the process for obtaining color-balanced karakul.
Method for increasing of egg hatching capacity and resistance of young farm birds / 2311023
Method involves providing triple treatment of eggs with glycin solution and succinic acid solution; spraying glycin solution and succinic acid solution onto egg shell surface; treating eggs before incubation and on 7th day of incubation with glycin solution; treating eggs on 19th day of incubation with succinic acid solution; using glycin in 0.5-1%-concentration and succinic acid in 0.3-0.5%-concentration; spraying said solutions onto 120-150 eggs during 6-12 hours before incubation at air temperature of 20-22 C within house.
 Modular apparatus for investigating animals' capability in achievement of reinforcement / 2311763
Apparatus has rectangular starting and target modules. Inlet and outlet openings are cut through side walls of starting module. Outlet openings are closed with doors. Each of four target modules comprises partition walls, inlet tunnels with aversive obstacles, and outlet corridors. Pedal positioned in front of inlet tunnel is connected through lock with door. Reinforcement is positioned within target part.
Method for obtaining unisexual female offspring in sturgeon fish / 2312495
The present innovation deals with induction of embryonic gynogenetic development due to inseminating ovicells with genetically inactivated sperm. Moreover, induction should be applied for ovicells of the females in the hybrids of phylogenetically distant species. Such females are able to produce unreduced diploid ovicells which are genetically identical to maternal ones. The innovation enables to create clonally reproducing female lines of sturgeon fish, (unisexual female offspring) in fish with different mechanisms of sex detection, the case when the mechanism is unknown, among them. Also, it gives the chance to considerably reduce expenses at forming a spawning school.
Method for increasing productivity of fur-producing animals / 2322052
Method involves introducing Emicidin antioxidant into ration of feed mixture in an amount of 25 mg per animal per day in two courses: beginning from last week of April - during 15 days and from first week of June - during 10-12 days.
 Method of wool quality assessment on fineness / 2328114
Method includes definition of a wool fineness of investigated animal with subsequent choice of selection direction on this indicator of wool productivity. In investigated wool in addition urea-bisulphite solubility is defined and estimation is made, defining graphically arrangement of crossing point of received results of a fineness (x, micron) and urea-bisulphite solubility of a wool (y, %) with the subsequent analysis of their placing concerning normed space limited by a line, formed on equation y = -ax+b, where y - urea-bisulphite solubility; a, b - factors calculated separately for each herd; x - fineness, received on average indexes of dependences for a wool of breeding and commodity animals, thus in case of received results finding on a line or above it, it is considered, that wool of investigated animal meets set requirements of quality on fineness and it is made decision to continue with this animal breeding work by an estimation wool production, otherwise - investigated animal is subject to a culling from a further breeding work in a wool productivity direction.
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FIELD: veterinary medicine. SUBSTANCE: method comprises individual testing on blood groups of both parents. Selection is then carried out taking into account the extent of their genetic differences, expressed in terms of antigenic similarity index ranging from 0 to 1. At that the morphologic condition of placenta is additionally determined, and the selection of parent pairs is carried out with the genetic compatibility of average index values of antigenic similarity in the range of Ra=0.31-0.60 and with the morphostructural condition of placenta weighting 310-330 g, the number of cotyledons of 68.4-76.2 pcs., the distance between the cotyledons of 2.2/2.5-2.7/3.0 cm. EFFECT: method enables to increase the birth rate of viable and highly productive offspring. 3 tbl, 4 ex
The technical field to which the invention relates. The invention relates to agriculture, namely, to livestock, to the way the selection of parental pairs in sheep, and can be used in different types of breeding animals of any species, populations targeted by selection on the basis of individual immunogenetic characteristics of blood and morphostructural characteristics of the placenta. The level of technology There is a method to assess the compatibility of the parent form, which reflects the phenotypic characteristics and productivity of animals (see Angolano, guidelines for breeding sheep masochistic and cold areas of productivity in the Russian Federation. - M, 2000 - 41 C.). The disadvantage of this method is the low precision of the estimate of the degree of compatibility of the parent forms. There is a method of assessing the degree of compatibility of parental forms on immunogenetic parameters, namely blood group (see Ullakko, Ivekovi. Individual selection of parental pairs / Ullakko, Ivekovi // Sheep. - 1991. No. 1. - s-23). The disadvantage of this method is the low precision of the estimate of the degree of compatibility of the parent forms. The known method the selection of parental pairs in breeding pigs, including the testing is the blood group and selection, taking into account their differences in antigenic factors of blood groups, using the genotypes at all loci blood groups, and to reproduce the selected parent pairs with difference coefficient of 0.55 and above (see U.S. Pat. RU # 2160987, IPC A01K 67/02, publ. 27.12.2000,). The disadvantage of this method is that the difference between animals take into account only the loci of blood groups that do not always give an accurate assessment of the degree of compatibility of the parent forms. The closest in technical essence and the achieved positive effect and adopted by the authors for the prototype is the method of selection of parental pairs for breeding cattle, including testing for blood groups and then selection, taking into account their differences in antigenic factors of blood groups, and for reproduction pair with index antigenic similarities, not exceeding 0,250 (see U.S. Pat. RU # 2112370, IPC A01K 67/02, publ. 10.06.1998,). The disadvantage of this method is that the difference between animals take into account only the loci of blood groups that do not always give an accurate assessment of the degree of compatibility of the parent forms. Disclosure of inventions The task of the invention is to develop a method of selection of parental pairs in sheep, has a high predictive effect by establishing limits on the optimum genetic compatibility of both parents and morphostructural state is lacenterra apparatus of the parent body. The technical result that can be achieved using the present invention is to increase fertility viable, productive offspring due to selection of parental pairs with regard to their degree of genetic compatibility and use of the placenta as bischoping material. The technical result is achieved by way of the selection of parental pairs in sheep, including individual testing for blood groups of both parents and then selection taking into account the degree of their genetic differences, expressed through the index antigenic similarities in the range from 0 to 1, while additionally determine morphostructural the condition of the placenta, and selection of parental pairs to determine if the genetic compatibility of the average values of the index antigenic similarities within Ra=0,31 is 0.60 and morphostructural condition of the placenta with mass 310-330 g, the number of cotyledons 68,4-76,2 pieces, the distance between cotyledonary 20/2,5-2,7/3,0, see The method of selection of parental pairs in sheep is as follows. During breeding company form parent pairs: sheep (♂)×uterus (females) of the three variants of the breed selection: option 1 - ST×CA: sheep (♂) Stavropol rocks (ST) uterus (females) Caucasian breed (KA); option 2 - CM×CA: sheep (♂) Soviet gelding is C (CM) uterus (females) Caucasian breed (KA); option 3 - AM×MM: sheep (♂) Australian Merinos (AM) uterus (females) manicacci Merino (MM). The mares and sheep produce blood from the carotid artery in tubes with sodium citrate in the amount of 3.8% in the ratio of 1 ml of citrate: 3 ml blood. Test tubes with blood, after thorough mixing, tightly closed tube and transported to the laboratory. From each blood sample serves 8-10 ml of a 2.5% suspension of erythrocytes. Using specific reagents immunodiagnostics and 2.5% suspension of erythrocytes of investigational animal put hematological tests, the reaction of hemolysis and agglutination, the results of which determine the antigenic composition of the blood of the animal on 6 systems loci of blood groups a, b, C, M, D, and 14 erythrocytic antigens AA, AB, CB, Be, Be, Bi, Bg, Ma, Ca, Cd, Da, O. Determine the individual genetic characteristics of animals, using the formula:
Ra - index antigenic similarities; S is the number of similar antigens, we compared animals; n1- the number of identified antigens for the first animal (father); n2- the number of identified antigens from the second animal (mother). Establish the degree of genetic compatibility between the rams and the uterus, which is expressed in numeric values of the Indus the KSA antigenic similarity from 0 to 1, additionally, postpartum uterine determine morphostructural the condition of the placenta and indicators morphostructural condition of the placenta, and selection of parental pairs to determine if the genetic compatibility of the average values of the index antigenic similarities within Ra=0,31 is 0.60 and morphostructural condition of the placenta with mass 310-330 g, the number of cotyledons 68,4-76,2 pieces, the distance between cotyledonary 20/2,5-2,7/3,0, see A brief description of the drawings and other materials Figure 1 is given to the selection of parental pairs in sheep table 1, the distribution of possible variants of a combination of parental pairs depending on the size of the index antigenic similarities. Figure 2 - the same, table 2, morphostructural indicators placenta females with the value of the index antigenic similarity of parental pairs. Figure 3 - same as table 3, live weight of lambs at birth, the number of re-insemination of Queen bees, depending on the size of the index antigenic similarities. The implementation of the invention Examples of specific implementation method the selection of parental pairs in sheep. Based on the analysis of modern animal husbandry, biological information concluded priority role for the evolution of living nature, objectively existing sexual selectivity. A great value selection, under the ora is that is the only factor that can produce a new biological structures - the birth of the offspring. While the male and female elements must be different, but to a certain extent (Malakhovsky YA screening and Selection methods and breeding of agricultural animals / Alamedacounty, Ayatolah. - Omsk, 1993. - 76 S.). Genetic system of blood groups is a collection of erythrocyte antigens. The sheep know 8 genetic systems, different number of antigens and the number of the corresponding alleles. Erythrocytic factors defined by hematologic tests, the reaction of hemolysis and illuminatii inherited by Mendeleev laws, immutable throughout the life of the animal, independent of the conditions of his detention, feeding inherited by descendants codominance represent a suitable model to identify differences in the genetic compatibility of parental pairs. The degree of these differences are expressed in the value of the index antigenic affinity, high value which indicates the genetic proximity of two individuals, low genetic divergence. The degree of genetic differences from 0 to 1 are calculated according to the formula (see Assurebuy. Genetic analysis / Assurebuy. - M.: Nauka, 1970. - 342 S.).
Ra - index antigenic similarities; S - h is words of similar antigens, we compared the animals; n1- the number of identified antigens for the first animal (father); n2- the number of identified antigens from the second animal (mother).
The method of selection of parental pairs in sheep is as follows. During breeding company form parent pairs: sheep (♂)×uterus (females) of the three variants of the breed selection: option 1 - ST×CA: sheep (♂) Stavropol rocks (ST) uterus (females) Caucasian breed (KA); option 2 - CM×CA: sheep (♂) Soviet Merino (CM) uterus (females) Caucasian breed (KA); option 3 - AM×MM: sheep (♂) Australian Merinos (S) uterus (females) manicacci Merino (MM). The mares and sheep produce blood from the carotid artery in tubes with sodium citrate in the number of 3.8% in the ratio of 1 ml of citrate: 3 ml blood. Test tubes with blood, after thorough mixing, tightly closed tube and transported to the laboratory. From each blood sample serves 8-10 ml of a 2.5% suspension of erythrocytes. Using specific reagents immunodiagnostics and 2.5% suspension of erythrocytes of investigational animal put hematological tests, the reaction of hemolysis and agglutination, the results of which determine the antigenic composition of the blood of the animal on 6 systems loci of blood groups a, b, C, M, D, and 14 erythrocytic antigens AA, AB, CB, Be, Be, Bi, Bg, AF, CA, Cd, Da, O. Determine the individual genetic characteristics of animals, using the formula above, establish the degree of genetic compatibility between the rams and the uterus, which is expressed in digital index values antigenic similarity from 0 to 1. The distribution of possible variants of a combination of parental pairs depending on the size of the index antigenic similarities are shown in table 1, Fig.1. Conducted calculations indicated the distribution of possible combinations of parental pairs in three ranges of values of the index antigenic similarity (IAS): in the interval 0-0,30; 0,31-0,60; 0,61-0,90. Among the examined animals the greatest number of parental pairs were distributed in the range of EAS from 0.30 to 0.60: for parent pairs ST×KA-64,7; CM×TO the - 60.2 and AM×MM - 54,4%. During lambing ewes taking placenta immediately after its exit from ancestral ways. The placenta weighed, consider the number of cotyledons, their size, the distance between them. Based on the analysis of the magnitude of the weight of the placenta, indicators cotyledonous apparatus of ewes with different values of the index antigenic similarities, established the superiority of the studied indicators of placental females with IAS with the average of its values (0,31 to 0.60), compared with the extreme options (0-0,30 and 0.61-0,90). Morphostructural indicators placenta females considering the magnitude of the IAS parental pairs shown in table 2, figure 2. In three different parent selection analyzed the magnitude of the body weight of newborn lambs and reproductive properties females. It was determined that females with IAS within 0,31-0,60 was born a larger number of lambs on average, 21.1%, with a greater magnitude of body weight on average, 16.7%, in the period of insemination of Queen bees was almost half the cases of re-insemination, but the birth of a greater number of twins on average of 51.1%. The live weight of lambs at birth, the number of re-insemination of Queen bees is shown in table 3, figure 3. Performance tested and proven on a representative material by verifying with the nature of the structure of the placenta, as bischoping material as is the body performs a major role in the birth of viable offspring. Thus, additional definition morphostructural placenta status in the selection of parental pairs with regard to their genetic compatibility, expressed through the index antigenic similarities contributed to the improvement of the reproductive qualities of the uterus and the birth of a large, viable offspring. The present invention in comparison with the prototype and other known technical solution has the following advantages: - increase of productivity of the young, due to selection of parental pairs, with an excellent combination of genetic parameters with additional assessment morphostructural placenta status; - ability to identify the optimal combination of parental forms in the early stages of pregnancy; - provide forecast of normal pregnancy and birth of viable offspring; - significant savings of time through the use of the placenta as cheap bischoping material. The method of selection of parental pairs in sheep, including individual testing for blood groups of both parents, and then the selection taking into account the degree of their genetic differences, expressed through the index antigenic similarities in the range from 0 to 1, characterized in that it further determine morphostructural the condition of the placenta, the rich selection of parental pairs is carried out at the genetic compatibility of the average values of index antigenic similarities within Ra=0,31 is 0.60 and morphostructural condition of the placenta with mass 310-330 g, the number of cotyledons 68,4-76,2 pieces, the distance between cotyledonary 2,2/2,5-2,7/3,0 see
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where