Method of increasing meat productivity of pigs by selection using genetic marker |
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IPC classes for russian patent Method of increasing meat productivity of pigs by selection using genetic marker (RU 2514634):
Method of increasing adaptive status of bee family / 2511304
Invention relates to livestock husbandry, in particular, to beekeeping for increasing the adaptive status of the bee family. The microdoses of biologically active substance are administered in the body of bees with water or an aqueous solution of carbohydrates. Dihydroquercetin is used as the biologically active substance.
Method for assessing stress resistance in pigs / 2510852
Invention refers to veterinary science and agriculture. The method provides pig's blood analysis for the following values: malondialdehyde MDA, antioxidant activity AOA, total oxidative activity of blood plasma TOA, catalase activity CT, superoxide dismutase SOD. The two-month pigs having MDA 6.02±0.13 absorption unit/ml and less, AOA 60.86±0.31% and more, TOA 71.64±0.16% and less, CT 57.30±1.05 absorption unit/ml min and more, SOD 14.57±0.44 absorption unit/ml and more are considered to be stress-resistant PSS "-"; the values of MDA 7.63±0.07 absorption unit/ml and more, AOA 50.22±0.42% and less, TOA 79.21±0.83% and more, CT 47.16±1.08 absorption unit/ml min and less, SOD 10.49±0.49 absorption unit/ml and less enables stating the pigs as stress-sensitive PSS "+". The six-month pigs having MDA 7.37±0.17 absorption unit/ml and less, AOA 61.93±0.25% and more, TOA 41.86±0.19% and less, CT 76.68±0.74 absorption unit/ml min and more, SOD 22.80±0.68 absorption unit/ml and more are considered to be stress-resistant PSS "-"; the values of MDA 8.05±0.09 absorption unit/ml and more, AOA 59.72±0.17% and less, TOA 46.21±0.09% and more, CT 71.76±0.51 absorption unit/ml min and less, SOD 19.91±0.59 absorption unit/ml and less enables stating the pigs as stress-sensitive PSS "+".
 Method of obtaining valid molecular-genetic model for pre-clinical tests of novel anti-epileptic medications / 2502257
Invention relates to field of medicine, neurobiology and pharmacokinetics and deals with method of obtaining valid molecular-genetic model of human absence epilepsy. Claimed method consists in the following: parent individuals P with genotype A1/A1 of gene DRD2 are identified by means of genotyping of Taq 1A DRD2 locus in rats of WAG/Rij line, crossed with each other with obtaining offspring F1, which is grown to reproductive age, after that, nonaudiogenic individuals are identified among offspring F1, after which nonaudiogenic individuals of offspring F1 are crossed with each other to obtain offspring F2, which is then also grown to reproductive age with the following selection of nonaudiogenic individuals among them, crossing and selection being performed repeatedly to obtain homogeneous population of nonaudiogenic rats of WAG/Rij line with genotype A1/A1 of gene DRD2, control of "ПВР" type in selected individuals of offspring F1 for further crossing is carried out by means of encephalographic analysis, which includes morphological control.
Method of selection of cattle in kalmyk breed according to meat productivity / 2498569
Invention relates to the field of genetics and animal breeding. The method of selection of cattle of Kalmyk breed is that at the age of 6-month the presence in blood of erythrocytic antigens-markers of tall-growing type: G2 E'3 R2 are detected. In the presence in the genotype of animals of markers of productivity the selection of animals is carried out.
 Young minks performance increase method / 2497371
Invention relates to fur farming field, in particular, to young minks performance increase. The fur animals growing method envisages introduction of biologically active substances (physiological promoters) into the ration of young minks intended for pelt obtainment. The biologically active substances (physiological promoters) are represented by L-carnitine preparation and are introduced in an amount of 30 mg per 1 kg of live weight into the ration in seven days' courses with seven days' intervals between them, once a day in the period of active growth - from June to August inclusive.
 Method of evaluation of broiler chickens / 2496315
Invention relates to the field of agriculture, namely, to selection, and can be used in breeding in poultry pedigree farms. Evaluation of broiler chickens is carried out according to the developed scale for fast and slow-fledging lines of broiler strains; the day-old chickens are distributed into three groups: 6-18 hours - late; 19-32 hours - average; 33 and above - early. The calculation of the duration of the embryonic development from laying to hatching chickens is carried out: the time is recorded from the start of incubation till evaluation of the chickens on development of fledging (T1), the age of chickens is subtracted from it, which is set according to a feather (T2), using the formula: T=T1-T2.
 Method of assessment of velvet antler yielding capacity of siberian red deers / 2491814
Invention relates to a velvet antler reindeer breeding and can be used to identify the productive qualities of Siberian red deers. The method is characterised in the fact that assessment is carried out according to the level of testosterone and/or estradiol secretion in blood serum in its sampling in any month from December to August inclusively, and Siberian red deers should be considered as highly productive with the following levels of secretion of testosterone in blood serum, nmol/l, and time period of its sampling: 1.67 - January, and/or 7.15 - March, and/or 5.41 - April, and/or 1.27 - June and/or 5.13 - August, not less and/or according to the level of estradiol secretion in blood serum, nmol/l, and time period of its sampling: 2.32 - December, and/or 2.91 - January, and/or 1.06 - March, and/or 1.50 - April, and/or 2.41 - June, and/or 2.68 - July, no more.
 Method to determine unfertilised eggs of drosophila / 2487934
Undeveloped eggs are placed for 45-50 minutes in a four percent solution of sodium hypochlorite (NaOCl), and on the basis of the number of dissolved eggs they define the quantity of unfertilised eggs. The proposed method makes it possible to perform mass investigations of quite large samples, to detect specifics at the level of frequency of occurrence of unfertilised eggs, both of genetically dependent factors and stresses of the environment of various nature at the reproductive system of the drosophila. Besides, the proposed method of chorion removal makes it possible to detect a share of parthenogenetically developing eggs, which is specific for certain types of drosophila, and also disturbances in the reproductive system of drosophila males. The method makes it possible to process quite a high quantity (100-200 pcs.) of undeveloped eggs referred to early embryonal parts within a short period of time, makes it possible to identify sterility of males. With the help of this method both for parent and several next generations of drosophila, they determine a prolonging effect of biological exposure of a stress agent at ability to leave posterity.
Method to increase yield of pigs / 2483534
Invention relates to veterinary science and pig breeding. The invention makes it possible to increase yield of pigs by application of a biological agent "Geprim for pigs" and "Gamavit". For this purpose the agent "Geprim for pigs" is injected to pigs once intramuscularly in the dose of 0.19-0.21 ml/kg, and "Gamavit" in the dose of 0.1-0.15 ml/kg per head in the beginning of fattening - on the first, fourth and ninth days.
 Method to determine genuineness of strain of animals - objects of agricultural purpose / 2477607
Invention relates to genetics and breeding of farm animals. The method provides for multiplex amplification of 13 loci of microsatellites of cattle (TGLA126, ILSTS005, ETH185, TGLA122, INRA023, ILSTS006, TGLA227, ETH225, ETH10, BM1818, BM1824, SPS115) using a test system for DNA-expertise of animals, building allelic profiles and subsequent calculation of similarity factor Q. At the same time for determination of genuineness of strain it is not required to previously determine allelic profiles of breeds and using reference populations. Those species are of genuine strain, which have similarity factor Q≥0.75.
 Method for detecting glycogen in extract out of organs and tissues in bees / 2256320
The present innovation deals with boiling an extract, cooling, centrifuging, dissolving a residue, cooling, centrifuging, dissolving a residue, adding sulfuric acid into a tube and 1%-condensate's solution followed by heating, cooling, photometry against the control at wave length being 315 nm, as a condensate one should apply resorcinol.
 Method for separating of synanthropic flies from substrate / 2290795
Method involves placing processed substrate with synanthropic fly larvae living thereon into reservoir by means of belt conveyor; setting velocity of belt conveyor so as to provide migration of larvae from substrate thickness into reservoir, onto its surface, said velocity depending on thickness of substrate layer: the greater is thickness of substrate layer , the lower is the velocity, and larvae concentration in upper part of reservoir being provided by making width of reservoir lower part exceeding width of its upper part; upon filling of reservoir, stopping feeding of substrate and removing larvae from upper part of reservoir by means of conveyor equipped with rectangular plates; removing substrate remained in reservoir by means of screw-type conveyor.
Method for producing of bird's gametes / 2294099
Method involves obtaining embryo stem cells from donor-kind birds and introducing stem cells in-ovo to recipient-kind birds, with recipient-kind birds differing from donor-kind birds; incubating recipient-kind cells to pecking stage; growing to sexual maturity phase and collecting gametes of donor-kind birds from recipient-kind birds.
Method for treatment of meat-type chicken eggs / 2308830
Method involves treating eggs with biologically active compositions, with organosilicon substances such as cresacin and meval being used as biologically active compositions. Said compositions are applied in the form of mixture of their solutions onto eggshell 6-12 hours before incubation.
Method for obtaining color-balanced karakul / 2310326
The present innovation deals with raising colored Karakul sheep and could be applied for obtaining qualitative, color-balanced, export-orientated karakul. It is necessary to select stud rams (lambs) aged 1-2 d to detect the peculiarities of distribution, the quantity and quantitative content of the pigment - melanin in hair. Additionally, it is important to carry out iridoscopy for the lambs aged 5-mo along with detection of color purity - the absence of stripes, lines, grooves, lacunes, spots and homogeneity - the absence of contrast distinctions in pigmentation of iris color. Then comes the selection of stud rams at steady distribution of pigment without any variations of color tone along the whole surface of iris and along its all projectional areas of the right and left eyes in 12.00-11.59 sector clockwise. Artificial insemination of females of the same color from these stud rams has been suggested followed by the analysis of the data obtained. The innovation enables to shorten the terms and cheapen the process for obtaining color-balanced karakul.
Method for increasing of egg hatching capacity and resistance of young farm birds / 2311023
Method involves providing triple treatment of eggs with glycin solution and succinic acid solution; spraying glycin solution and succinic acid solution onto egg shell surface; treating eggs before incubation and on 7th day of incubation with glycin solution; treating eggs on 19th day of incubation with succinic acid solution; using glycin in 0.5-1%-concentration and succinic acid in 0.3-0.5%-concentration; spraying said solutions onto 120-150 eggs during 6-12 hours before incubation at air temperature of 20-22 C within house.
 Modular apparatus for investigating animals' capability in achievement of reinforcement / 2311763
Apparatus has rectangular starting and target modules. Inlet and outlet openings are cut through side walls of starting module. Outlet openings are closed with doors. Each of four target modules comprises partition walls, inlet tunnels with aversive obstacles, and outlet corridors. Pedal positioned in front of inlet tunnel is connected through lock with door. Reinforcement is positioned within target part.
Method for obtaining unisexual female offspring in sturgeon fish / 2312495
The present innovation deals with induction of embryonic gynogenetic development due to inseminating ovicells with genetically inactivated sperm. Moreover, induction should be applied for ovicells of the females in the hybrids of phylogenetically distant species. Such females are able to produce unreduced diploid ovicells which are genetically identical to maternal ones. The innovation enables to create clonally reproducing female lines of sturgeon fish, (unisexual female offspring) in fish with different mechanisms of sex detection, the case when the mechanism is unknown, among them. Also, it gives the chance to considerably reduce expenses at forming a spawning school.
Method for increasing productivity of fur-producing animals / 2322052
Method involves introducing Emicidin antioxidant into ration of feed mixture in an amount of 25 mg per animal per day in two courses: beginning from last week of April - during 15 days and from first week of June - during 10-12 days.
 Method of wool quality assessment on fineness / 2328114
Method includes definition of a wool fineness of investigated animal with subsequent choice of selection direction on this indicator of wool productivity. In investigated wool in addition urea-bisulphite solubility is defined and estimation is made, defining graphically arrangement of crossing point of received results of a fineness (x, micron) and urea-bisulphite solubility of a wool (y, %) with the subsequent analysis of their placing concerning normed space limited by a line, formed on equation y = -ax+b, where y - urea-bisulphite solubility; a, b - factors calculated separately for each herd; x - fineness, received on average indexes of dependences for a wool of breeding and commodity animals, thus in case of received results finding on a line or above it, it is considered, that wool of investigated animal meets set requirements of quality on fineness and it is made decision to continue with this animal breeding work by an estimation wool production, otherwise - investigated animal is subject to a culling from a further breeding work in a wool productivity direction.
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FIELD: agriculture. SUBSTANCE: invention relates to the field of agriculture. The method comprises selection of pigs using a genetic marker. At that the selection and choosing of rearing stock is carried out at achievement of live weight of 90-100 kg in the degree of concentration of free lysine in the muscle tissue of longissimus muscle of back (Musculus Longissimus Dorsi). The animals with the level of lysine concentration of 1.36 mmol/kg of fresh weight and more are left in a breed. EFFECT: use of the method enables to improve the meat productivity of pigs. 1 tbl
The present invention relates to animal husbandry and is a way of breeding for the genetic marker. Currently it is mainly indirect selection based on the selection by individual characteristics with the phenotypic manifestation in them of the actions of genes, precluding the selection of specific genetic factors, but reduces the effect of selection and slow down the process. There is, for example, a method of breeding pigs to increase growth rate and meat yield based on the prediction of their type using the ratio of the decline in the rate of growth precocity piglets (patent No. 2271102, A01K 67/02, 2006). However, these traits have low heritability, and therefore a large variability under the influence of the paratypical factors, and selection on them ineffective. Method of control fattening animals (patent No. 2118085, A01K 67/02, 1998) time-consuming, requires a lot of production capacity, wasteful in the use of breeding stock: up to 50% similar offspring (siblings) is sent to slaughter. The method of selection of animals for traits that are inherited by the mechanism of parental imprinting (patent No. 2262229, A01K 67/02, C12Q 1/68, 2000), does not reflect the frequency of allelic genes in the nucleotide sequence of the DNA molecule, and thus casts doubt on the perfection of the effect is vnesti selection. Dynamic selection processes are within the sphere of influence the transcription of DNA into RNA, which is based on the mechanism of action of the present invention. During its preparation the task was instead mediated selection to develop a way to carry out this process not on circumstantial evidence, and measured their genetic conditionality, as, for example, the concentration of the substance. Recommended way to increase meat sow productivity through breeding using as a genetic marker of the level of concentration of the amino acid lysine in the muscle tissue of animals. This amino acid is an important part of nuclear histone proteins associated with DNA in chromosomes, is part of the ribosomes, enzymes that catalyze DNA and RNA involved in the implementation of genetic information. The current link in the lysine codons is a purine base adenine. Oligomeric for adenine, lysine together with phenylalanine plays a big role in the reproduction of the precursors of DNA, accelerating the reproduction of the messenger RNA that encodes a protein synthesis. The triplet AAA lysine in another DNA chain corresponds to the complementary timiney triplet TTT, replaced mRNA on oralloy triplet YYY phenylalanine. Unlike other amino acids lysine has the ability of the Yu to increase its concentration in the muscle tissue while increasing meat animal productivity as a high and low speed their growth. Obviously, this property determines the ability of lysine to increase meat productivity of pigs and to serve as a genetic marker amplification processes of protein synthesis and muscle tissue. This scientific conclusion is proved in experiments in breeding pigs in two adjacent generations. The concentration of lysine as a genetic marker was determined by the principle of the optimal model in accordance with theory of high growth rate of pigs (Boars, 2005). The test concentration was determined on the background of two of the most important in experimental breeding traits: growth rate (the amount of increase in the early stages of ontogenesis) and meat productivity. Scheme for the construction of models: animals selected for high () moderate () growth rate, each of which, in turn, were divided into two subgroups, selectionservice in the direction of meat (M) or fat (G) productivity. Therefore, checking the level of concentration of free lysine was carried out on the background of the two most important factors (biological reserves) growth and development: 1) the intensity of growth in pigs 10-15 times higher than that of agricultural animals of other species (e.g., in the period of ontogenesis in cattle live weight in polutropos development increases 10-12 times, and pigs is more than 200 times); 2) content of the muscle tissue in the body genetically lysine. Established that the reserves growth are high negative correlation r= - 0.57, and R=-0,022: with the increase of gain in live weight of 1 g per day, the content of lean meat in the body decreases by 0,022, Therefore, heredity negatively correlated traits is when their transgressive variation beyond permissions levels (the law of hardy-Weinberg equilibrium). When purposeful selection heredity one negative correlated trait "burden" another symptom. Greatly increases the rigidity of the selection of meat characteristics under the influence of multifactorial influences. For signs of fat is influenced by Mnogovershinnoye amino acids valine, glycine, Proline, serine, causing lipoproteine synthesis, increased body inclusions in majuscule space of muscle tissue, increasing its fat and overall weight. Considering the above, in order to increase meat productivity of pigs minimal level of concentration of free lysine in muscle tissue as a genetic marker for selection of pigs should be considered of 1.36 mmol/kg wet weight. Calculations show that in the selection of pigs at a specified rate of lysine increased average daily nature of the TA to 200 g per day, with regard to regression - to 0.022, meat productivity results boning carcasses of animals with the level of growth 730 g will be 56,1%. And in the case of selection of animals on the concentration of lysine 1.70 mmol/kg, as shown in the table of applications and increase average daily weight gain up to 713 g, the proportion of meat in the carcass will be to 59.6%. In experimental breeding to increase animal productivity pigs breeding shift the content of the muscle tissue in the carcass in each of the two generations amounted to 2.25%. Literature 1. Patent of Russia №2271102 - A01K 67/02, 2006 2. Patent of Russia №2118085 - AC 67/02, 1998 3. Patent of Russia №226229 - A01K 67/02, C12Q 1/68, 2000
The way to increase meat productivity of pigs way of the selection using a genetic marker is selected and the selection of replacements when reaching a body weight of 90-100 kg according to the degree of concentration of free lysine in the muscle tissue of the longest muscle back (Musculus Longissimus Dorsi), the tribe leave the animals with the level of lysine concentration of 1.36 mmol/kg wet tissue and more.
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