Method of stimulating embryogenesis of laying hens |
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IPC classes for russian patent Method of stimulating embryogenesis of laying hens (RU 2520092):
 Regulation of productive characteristics in birds / 2518681
Invention relates to field of biotechnology. Method includes introduction of RNA molecule into a bird egg. Introduced RNA molecule contains double-stranded region and results in reduction of the level of molecule of RNA and/or protein, included into determination of sex in birds, in the egg. Invention can be used in poultry breeding.
Method of increasing meat productivity of pigs by selection using genetic marker / 2514634
Invention relates to the field of agriculture. The method comprises selection of pigs using a genetic marker. At that the selection and choosing of rearing stock is carried out at achievement of live weight of 90-100 kg in the degree of concentration of free lysine in the muscle tissue of longissimus muscle of back (Musculus Longissimus Dorsi). The animals with the level of lysine concentration of 1.36 mmol/kg of fresh weight and more are left in a breed.
Method of increasing adaptive status of bee family / 2511304
Invention relates to livestock husbandry, in particular, to beekeeping for increasing the adaptive status of the bee family. The microdoses of biologically active substance are administered in the body of bees with water or an aqueous solution of carbohydrates. Dihydroquercetin is used as the biologically active substance.
Method for assessing stress resistance in pigs / 2510852
Invention refers to veterinary science and agriculture. The method provides pig's blood analysis for the following values: malondialdehyde MDA, antioxidant activity AOA, total oxidative activity of blood plasma TOA, catalase activity CT, superoxide dismutase SOD. The two-month pigs having MDA 6.02±0.13 absorption unit/ml and less, AOA 60.86±0.31% and more, TOA 71.64±0.16% and less, CT 57.30±1.05 absorption unit/ml min and more, SOD 14.57±0.44 absorption unit/ml and more are considered to be stress-resistant PSS "-"; the values of MDA 7.63±0.07 absorption unit/ml and more, AOA 50.22±0.42% and less, TOA 79.21±0.83% and more, CT 47.16±1.08 absorption unit/ml min and less, SOD 10.49±0.49 absorption unit/ml and less enables stating the pigs as stress-sensitive PSS "+". The six-month pigs having MDA 7.37±0.17 absorption unit/ml and less, AOA 61.93±0.25% and more, TOA 41.86±0.19% and less, CT 76.68±0.74 absorption unit/ml min and more, SOD 22.80±0.68 absorption unit/ml and more are considered to be stress-resistant PSS "-"; the values of MDA 8.05±0.09 absorption unit/ml and more, AOA 59.72±0.17% and less, TOA 46.21±0.09% and more, CT 71.76±0.51 absorption unit/ml min and less, SOD 19.91±0.59 absorption unit/ml and less enables stating the pigs as stress-sensitive PSS "+".
 Method of obtaining valid molecular-genetic model for pre-clinical tests of novel anti-epileptic medications / 2502257
Invention relates to field of medicine, neurobiology and pharmacokinetics and deals with method of obtaining valid molecular-genetic model of human absence epilepsy. Claimed method consists in the following: parent individuals P with genotype A1/A1 of gene DRD2 are identified by means of genotyping of Taq 1A DRD2 locus in rats of WAG/Rij line, crossed with each other with obtaining offspring F1, which is grown to reproductive age, after that, nonaudiogenic individuals are identified among offspring F1, after which nonaudiogenic individuals of offspring F1 are crossed with each other to obtain offspring F2, which is then also grown to reproductive age with the following selection of nonaudiogenic individuals among them, crossing and selection being performed repeatedly to obtain homogeneous population of nonaudiogenic rats of WAG/Rij line with genotype A1/A1 of gene DRD2, control of "ПВР" type in selected individuals of offspring F1 for further crossing is carried out by means of encephalographic analysis, which includes morphological control.
Method of selection of cattle in kalmyk breed according to meat productivity / 2498569
Invention relates to the field of genetics and animal breeding. The method of selection of cattle of Kalmyk breed is that at the age of 6-month the presence in blood of erythrocytic antigens-markers of tall-growing type: G2 E'3 R2 are detected. In the presence in the genotype of animals of markers of productivity the selection of animals is carried out.
 Young minks performance increase method / 2497371
Invention relates to fur farming field, in particular, to young minks performance increase. The fur animals growing method envisages introduction of biologically active substances (physiological promoters) into the ration of young minks intended for pelt obtainment. The biologically active substances (physiological promoters) are represented by L-carnitine preparation and are introduced in an amount of 30 mg per 1 kg of live weight into the ration in seven days' courses with seven days' intervals between them, once a day in the period of active growth - from June to August inclusive.
 Method of evaluation of broiler chickens / 2496315
Invention relates to the field of agriculture, namely, to selection, and can be used in breeding in poultry pedigree farms. Evaluation of broiler chickens is carried out according to the developed scale for fast and slow-fledging lines of broiler strains; the day-old chickens are distributed into three groups: 6-18 hours - late; 19-32 hours - average; 33 and above - early. The calculation of the duration of the embryonic development from laying to hatching chickens is carried out: the time is recorded from the start of incubation till evaluation of the chickens on development of fledging (T1), the age of chickens is subtracted from it, which is set according to a feather (T2), using the formula: T=T1-T2.
 Method of assessment of velvet antler yielding capacity of siberian red deers / 2491814
Invention relates to a velvet antler reindeer breeding and can be used to identify the productive qualities of Siberian red deers. The method is characterised in the fact that assessment is carried out according to the level of testosterone and/or estradiol secretion in blood serum in its sampling in any month from December to August inclusively, and Siberian red deers should be considered as highly productive with the following levels of secretion of testosterone in blood serum, nmol/l, and time period of its sampling: 1.67 - January, and/or 7.15 - March, and/or 5.41 - April, and/or 1.27 - June and/or 5.13 - August, not less and/or according to the level of estradiol secretion in blood serum, nmol/l, and time period of its sampling: 2.32 - December, and/or 2.91 - January, and/or 1.06 - March, and/or 1.50 - April, and/or 2.41 - June, and/or 2.68 - July, no more.
 Method to determine unfertilised eggs of drosophila / 2487934
Undeveloped eggs are placed for 45-50 minutes in a four percent solution of sodium hypochlorite (NaOCl), and on the basis of the number of dissolved eggs they define the quantity of unfertilised eggs. The proposed method makes it possible to perform mass investigations of quite large samples, to detect specifics at the level of frequency of occurrence of unfertilised eggs, both of genetically dependent factors and stresses of the environment of various nature at the reproductive system of the drosophila. Besides, the proposed method of chorion removal makes it possible to detect a share of parthenogenetically developing eggs, which is specific for certain types of drosophila, and also disturbances in the reproductive system of drosophila males. The method makes it possible to process quite a high quantity (100-200 pcs.) of undeveloped eggs referred to early embryonal parts within a short period of time, makes it possible to identify sterility of males. With the help of this method both for parent and several next generations of drosophila, they determine a prolonging effect of biological exposure of a stress agent at ability to leave posterity.
 Method for detecting glycogen in extract out of organs and tissues in bees / 2256320
The present innovation deals with boiling an extract, cooling, centrifuging, dissolving a residue, cooling, centrifuging, dissolving a residue, adding sulfuric acid into a tube and 1%-condensate's solution followed by heating, cooling, photometry against the control at wave length being 315 nm, as a condensate one should apply resorcinol.
 Method for separating of synanthropic flies from substrate / 2290795
Method involves placing processed substrate with synanthropic fly larvae living thereon into reservoir by means of belt conveyor; setting velocity of belt conveyor so as to provide migration of larvae from substrate thickness into reservoir, onto its surface, said velocity depending on thickness of substrate layer: the greater is thickness of substrate layer , the lower is the velocity, and larvae concentration in upper part of reservoir being provided by making width of reservoir lower part exceeding width of its upper part; upon filling of reservoir, stopping feeding of substrate and removing larvae from upper part of reservoir by means of conveyor equipped with rectangular plates; removing substrate remained in reservoir by means of screw-type conveyor.
Method for producing of bird's gametes / 2294099
Method involves obtaining embryo stem cells from donor-kind birds and introducing stem cells in-ovo to recipient-kind birds, with recipient-kind birds differing from donor-kind birds; incubating recipient-kind cells to pecking stage; growing to sexual maturity phase and collecting gametes of donor-kind birds from recipient-kind birds.
Method for treatment of meat-type chicken eggs / 2308830
Method involves treating eggs with biologically active compositions, with organosilicon substances such as cresacin and meval being used as biologically active compositions. Said compositions are applied in the form of mixture of their solutions onto eggshell 6-12 hours before incubation.
Method for obtaining color-balanced karakul / 2310326
The present innovation deals with raising colored Karakul sheep and could be applied for obtaining qualitative, color-balanced, export-orientated karakul. It is necessary to select stud rams (lambs) aged 1-2 d to detect the peculiarities of distribution, the quantity and quantitative content of the pigment - melanin in hair. Additionally, it is important to carry out iridoscopy for the lambs aged 5-mo along with detection of color purity - the absence of stripes, lines, grooves, lacunes, spots and homogeneity - the absence of contrast distinctions in pigmentation of iris color. Then comes the selection of stud rams at steady distribution of pigment without any variations of color tone along the whole surface of iris and along its all projectional areas of the right and left eyes in 12.00-11.59 sector clockwise. Artificial insemination of females of the same color from these stud rams has been suggested followed by the analysis of the data obtained. The innovation enables to shorten the terms and cheapen the process for obtaining color-balanced karakul.
Method for increasing of egg hatching capacity and resistance of young farm birds / 2311023
Method involves providing triple treatment of eggs with glycin solution and succinic acid solution; spraying glycin solution and succinic acid solution onto egg shell surface; treating eggs before incubation and on 7th day of incubation with glycin solution; treating eggs on 19th day of incubation with succinic acid solution; using glycin in 0.5-1%-concentration and succinic acid in 0.3-0.5%-concentration; spraying said solutions onto 120-150 eggs during 6-12 hours before incubation at air temperature of 20-22 C within house.
 Modular apparatus for investigating animals' capability in achievement of reinforcement / 2311763
Apparatus has rectangular starting and target modules. Inlet and outlet openings are cut through side walls of starting module. Outlet openings are closed with doors. Each of four target modules comprises partition walls, inlet tunnels with aversive obstacles, and outlet corridors. Pedal positioned in front of inlet tunnel is connected through lock with door. Reinforcement is positioned within target part.
Method for obtaining unisexual female offspring in sturgeon fish / 2312495
The present innovation deals with induction of embryonic gynogenetic development due to inseminating ovicells with genetically inactivated sperm. Moreover, induction should be applied for ovicells of the females in the hybrids of phylogenetically distant species. Such females are able to produce unreduced diploid ovicells which are genetically identical to maternal ones. The innovation enables to create clonally reproducing female lines of sturgeon fish, (unisexual female offspring) in fish with different mechanisms of sex detection, the case when the mechanism is unknown, among them. Also, it gives the chance to considerably reduce expenses at forming a spawning school.
Method for increasing productivity of fur-producing animals / 2322052
Method involves introducing Emicidin antioxidant into ration of feed mixture in an amount of 25 mg per animal per day in two courses: beginning from last week of April - during 15 days and from first week of June - during 10-12 days.
 Method of wool quality assessment on fineness / 2328114
Method includes definition of a wool fineness of investigated animal with subsequent choice of selection direction on this indicator of wool productivity. In investigated wool in addition urea-bisulphite solubility is defined and estimation is made, defining graphically arrangement of crossing point of received results of a fineness (x, micron) and urea-bisulphite solubility of a wool (y, %) with the subsequent analysis of their placing concerning normed space limited by a line, formed on equation y = -ax+b, where y - urea-bisulphite solubility; a, b - factors calculated separately for each herd; x - fineness, received on average indexes of dependences for a wool of breeding and commodity animals, thus in case of received results finding on a line or above it, it is considered, that wool of investigated animal meets set requirements of quality on fineness and it is made decision to continue with this animal breeding work by an estimation wool production, otherwise - investigated animal is subject to a culling from a further breeding work in a wool productivity direction.
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FIELD: agriculture. SUBSTANCE: method comprises a single treatment of hatching eggs before laying into the incubator from sprayer with the aerosol of 0.01% sodium salt solution of lipoic acid, which is preliminary dissolved and mixed in distilled water at a temperature of 18-22°C. The solution is applied to the surface of egg shells 3-4 hours prior to their incubation. EFFECT: method enables to reduce lipid peroxidation processes, to optimise the metabolic rate, to increase the intensity of hatching at high viability of young birds for a long period of post-embryonic development. 5 tbl, 4 ex
This invention relates to the field of agriculture, particularly poultry, and can be applied for the prevention of technological stress in laying hens egg breeds in the critical periods of development of the chicken embryo. It is known that the effect of technological stress leads to the activation of free-radical processes [4] and increases lipid peroxidation in the tissues of chicken embryos, which causes significant economic damage to the poultry industry [1, 2]. It is therefore important prevent these phenomena using environmentally safe natural metabolites [3, 5]. The sodium salt of a-lipoic acid for the treatment of hatching eggs is unknown. Lipoic acid and its salts actively used in medicine, but in agriculture, its use is extremely small. Sodium salt of lipoic acid is involved in the oxidative decarboxylation of pyruvic acid and a - ketoacids, in the process of formation of energy in the body, in the regulation of lipid and carbohydrate metabolism, has a lipotropic effect, affects the metabolism of cholesterol, improves liver function [6]. The sodium salt of lipoic acid due to its good solubility in water, the lipoic acid is insoluble in water. It should be noted that this metabolite has never been used to prevent those of the ideological stress in poultry, as well as its influence on the chicken embryo is not known. The purpose of the invention is the prevention of technological stress in laying hens egg breeds in the critical periods of development of the chicken embryo by reducing processes lipoperoxidative, optimization of metabolism. The technical result is stimulation of embryonic development, egg chickens, increase output conforming chickens, prevention of technological stress. This result is achieved by reducing processes lipoperoxidation and optimize the level of metabolism during critical periods of development of the chicken embryo, by means of a single processing of hatching eggs, namely, before placing in the incubator aerosol spray on the surface of egg shells cause a 0.01% solution of sodium salt of lipoic acid. The method is as follows: On the surface of the shell for 3-4 hours prior to incubation of the spray is applied an aqueous solution of sodium salt of lipoic acid in a concentration of 0.01%. Previously the drug is dissolved and mixed in distilled water at a temperature of 18-22°C. Example 1. Eggs of the cross "shaver Braun was received from one parent stock, sorted by 306 pieces in each batch according to the principle of analogues with respect to time of the demolition and retention and weight. On the surface of the shell struck in the form of AE is the open air the solution under the above scheme. As control group were selected eggs of the same batch on the principle of analogues, which were processed according to the technology adopted in the economy. The obtained data are presented in table 1. The table shows that the conclusion of an experimental batch exceeds the control 11,77%and hatchability on 11,03%. Significantly decreased such waste incubation: "frozen" 4 times, "Sadochok" 3 times, the "weak" 5.1 times.
Example 2. The laying of the eggs of the cross "shaver Braun" on the incubation of the test and control groups was 05.03.2012, at 12 o'clock. 25.03.2012, began to withdraw, the chord continues 29 hours, which is 9 hours ahead of the normal output. Thus, the development of the allantois in the experimental group on the 11th day ahead of control that can be seen from table 2.
Example 3. Chickens derived from treated eggs (example 1), were grown in the same household up to 30 days of age in cage batteries. The data presented in table 3. In the experimental group for the entire study period increased the safety of the young at the 1%, respectively, compared to control. save.
Example 4. The Chicks hatched from the treated eggs, day old for biochemical analysis serum installed: the content of total protein in the serum of Chicks of the experimental group increased by 5.3%, α-amylase by 8.2%, blood glucose 0.9%, increase lysozyme activity by 4.6%. The data presented in table 4.
As can be seen from table 5 the drug has antioxidant action in the body of the chicken. The table shows that the intensity of peroxidation in experimental group is lower than in the control. Thus, the secondary product of lipid peroxidation in the form of malonic dialdehyde (MDA) decreased by 12.5%. Also in reducing the intensity of the processes of lipoperoxidation, increased antioxidant enzymatic defenses of day-old Chicks, which resulted in the increase of superoxide dismutase (SOD) in 1.5 times. All bio is himicheskie indicators were within the physiological norm.
Thus, on the basis of the performed experiments, there is an increase in the intensity of output with high viability young for a long period of postembryonic development. When this chick in an experimental batch was higher on 11,77% (p<0,001), and hatchability of eggs on 11,03% (p<0,001) compared with the control group. Literature 1. Geekin A.P. Industrial poultry / AP. Geekin, Fax, A.V. Aralov etc. // Ed. by V.I. Fisinin V.E., Sergiev Posad.: Wildebeest breeding of Rosselchozacademy is. - 2010. - 599 S. 2. Baraboi, VA Peroxidation and stress / VA Baraboi, I.I. Brekhman, SHE Gelatin, SHE Kudryashov // SPb. - Science. - 1992. - 148 C. 3. Burlakov, S. the Influence of biologically safe ways of handling eggs on embryonic development of chickens / S. Burlakov // the age of three. proc. young scientists and postgraduate students poultry, abstracts of papers. - Sergiev-Posad. - 1999. - 11 S. 4. Vladimirov, Y.A. Free radicals in living systems/ Y.A. Vladimirov, O.A. Azizov, Aigai, A.V. Kozlov, A.N. Osipov, DI Roshchupkin // Results of Science and Technology. series Biophysics. - Tom. - Moscow. - VINITI. - 1992. - 250 S. 5. Yermolov US Handling eggs chickens biologically active drugs to stimulate the resistance of chickens at various stages of ontogenesis: abstract of thesis. Kida. Biol. Sciences. / Yermolov YU.S. // M - 2010. - P.23. 6. IPR Joseph. Alpha lipoic acid is a universal remedy against free radicals, cell aging / Joseph IPRs // M: Dilya. - 2009. - 96 S. The method of stimulation of embryogenesis egg hens during critical periods of development of the chicken embryo by reducing processes lipoperoxidative, optimize metabolism, increase the intensity of output with high viability young for a long period of postembryonic development, including one-time processing of hatching eggs, in particular, before placing in the incubator and Rosalina from the sprinklers on the surface of shell eggs for 3-4 hours before incubation put a 0.01% solution of sodium salt of lipoic acid, which is to be dissolved and mixed in distilled water at a temperature of 18-22°C.
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