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Method of producing sulphated arabinogalactan derivatives. RU patent 2466143. |
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FIELD: chemistry. SUBSTANCE: invention relates to methods of producing arabinogalactan-based sulphated biopolymers. The method involves reaction of arabinogalactan with a sulphating SO3-pyridine complex while continuously stirring and heating, and separating the product. The arabinogalactan first undergoes mechanochemical treatment in pyridine with ratio of the mass of arabinogalactan to the volume of pyridine equal to 1:10 for 10 minutes. Sulphation is carried out at temperature 50-55°C for 40-60 minutes. The product is separated by neutralising with aqueous solution of an alkali and then precipitated in ethyl alcohol. EFFECT: invention increases efficiency of sulphation process, cut its duration and reduce reaction temperature. 5 ex
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β-cyclodextrine clathrate complex with 5-hydroxy-4-aminomethyl-1 -cyclohexyl(or cycloheptyl)-3-alkoxycarbonylindole derivative, method for preparing it (versions), pharmaceutical composition and drug / 2464042 Invention refers to a new β-cyclodextrine clathrate complex (an inclusion compound) with 5-hydroxy-4-aminomethyl-1-cyclohexyl(or cycloheptyl)-3-alkoxycarbonylindole derivative: β-cyclodextrine 1:1 to 1:5, preferentially at the relation of 1:1 to 1:3 of general formula (I): wherein X means - hydrogen, chlorine, iodine, n=1 or 2, R3-C1-C3 alkyl, ALK means C1-C6 alkyl group, R1, R2 are independently specified in C1-C4-alkyl, preferentially methyl, or R1 and R2 together with a nitrogen atom (i.e. group - NR1R2) means the groups described by formulas: wherein Bn is benzyl, a Ph is phenyl with the molar ratio of 5-hydroxy-4-aminomethyl-1-cyclohexyl(or cycloheptyl)-3-alkoxycarbonylindole derivative: β-cyclodextrine 1:1 to 1:5, preferentially 1:1 to 1:3, especially preferentially in the relation of 1:2. The clathrate complex may represent nanoparticles of size not less than 100 nm. There are preferential clathrate complexes wherein 5-hydroxy-4-aminomethyl-1-cyclohexyl(or cycloheptyl)-3-alkoxycarbonylindole derivative represents 1-cyclohexyl-4-aminomethyl-5-hydroxy-2-methyl-1H-indole-3-carboxylic acid ethyl ester. The new clathrate complexes possess antiviral action and exhibit high activity versus influenza viruses. The invention also involves a pharmaceutical composition and a drug based on the clathrate complexes. Besides, the invention refers to liquid-phase and solid-phase synthesis of the clathrate complexes. |
Method for hydrophobic modification of exopolysaccharides / 2463353 Disclosed is a method for hydrophobic modification of exopolysaccharides selected from xanthan and ritizan. The H+ form of exopolysaccharide is obtained. The obtained H+ form of exopolysaccharide then reacts with a primary alkyl amine in an aprotic solvent in the presence of dicyclohexyl carbodiimide. The aprotic solvent used is 1-methyl-2-pyrrolidone, dimethyl formamide and dimethylsulphoxide. The modified exopolysaccharide is separated by multiple precipitation. |
Method for forming chitosan film coating and chitosan film coating / 2461575 Chitosan is dissolved in an organic acid: 4-6% citric acid or 2-8% lactic acid in the relation of the ingredients chitosan: the organic acid 1:2-1:4 to prepare a forming solution. Chitosan has molecular weight 80-500 kDa. The forming solution is added with vitamin B1 in the amount of max. 0.5 wt %. The prepared forming solution is applied on a substrate in the amount of 0.2-0.25 ml/cm2 and kept to achieve a film structure. Said method is used to form the chitosan film coating having the thickness of 50-250 mcm and the breaking elongation of 42 to 470%. |
Ballast substance / 2460312 Inventions group relates to biotechnology. The ballast substance as a food additive for the human and animals with a monolocular stomach contains a fibre composition including the following components: lignocellulose, a fermentable fibre ingredient (bark material of soft wood, hardwood or their mixtures) and a marginally fermentable or non-fermentable fibre ingredient (fibres of soft wood, hardwood and their mixtures) at a ratio of 1:100 - 100:1, isoquinoline alkaloids. The ballast substance is produced and purified; impurities are removed at stage (a). The components are dried till dry substances content is equal to 3-12% at stage (b), milled, strained at stage (c) and mixed. The mixture is pressed at stage (d) and milled into desired size particles and mixed at stage (e). One adds a vegetable material containing isoquinoline alkaloids before pressing at stage d) and before mixing the ground components at stage (e). According to the second version of the method isoquinoline alkaloids are added at the stages of mixing (iv) or pressing and grinding at stage (v). The ballast substance is included in the composition of a fodder (in an amount of 0.01 - 50 % of the total fodder weight) and a fodder premix (up to >99%). The ballast substance is applied as a food additive in human alimentation, as a fodder or food additive for weight reduction. |
Method of producing chondroitin sulphate from sea hydrobiont tissue / 2458134 Method involves preparation of material for enzymatic hydrolysis. Alkaline hydrolysis is carried out with proteolytic enzyme preparations with neutralisation of the obtained solution to pH=7. A salt is added to the obtained enzymatic hydrolysate to a value of not less than 0.1 mol/l. Successive ultrafiltration is carried out, first on a membrane with maximum retention of 50 kD with separation of high-molecular weight impurities, and then on a membrane with maximum retention of 5 kD with separation of low-molecular weight substances. The chondroitin sulphate solution retained at the membrane is washed on the same membrane with distilled water until complete removal of salts. Final washing with distilled water is carried out on a membrane with maxim retention of 50 kD. |
Cross-linked hyaluronic acid and production method thereof / 2456299 Method involves activation of hyaluronic acid using a cross-linking agent and an auxiliary cross-linking agent. The activated hyaluronic acid then reacts with a nucleophilic cross-linking agent. The pH of the reaction medium ranges from 8 to 12. The nucleophilic cross-linking agent contains at least 50 wt % oligopeptide or polypeptide. Further, pH of the reaction medium is regulated to 5-7 and cross-linked hyaluronic acid is precipitated in the organic solvent. The invention also relates to use of the cross-linked hyaluronic acid obtained using this method in plastic surgery to make implants and to a hedrogel containing said cross-linked hyaluronic acid in a buffer aqueous solvent. |
Method for producing gel-forming dextrane phosphates / 2455007 Invention refers to chemical-pharmaceutical industry, medicine, biotechnology, and concerns a method for producing dextrane phosphate hydrogels which can find application in preparing prolonged preparations for treating oncological diseases, infectious diseases, immune disorders. The declared invention describes the method for producing polysaccharide phosphate hydrogels, involving processing of an initial polysaccharide in mixed orthophosphoric acid and phosphorus oxide (V) in an organic solvent medium in the presence of trialkylphosphate at temperature 30-70°C that is followed by washing of the prepared products, drying and processing in an aqueous solution of a mineral compound with the etherification process of dextrane conducted in a non-polar organic solvent medium of the low boiling point specified in hydrocarbons and their derivatives and processing of the prepared product in 0.005-0.5 M aqueous solutions of sodium carbonate, or sodium hydrocarbonate, or sodium hydroxide, or their mixtures to the value pH 3.0-8.0 to be set down in ethanol and dried at temperature 20-50°C. |
Method of producing arabinogalactan / 2454429 Invention relates to methods of producing arabinogalactan. The method involves aqueous extraction of arabinogalactan from resin-free larchwood while heating. The obtained arabinogalactan extract is purified from high-molecular weight impurities by ultra filtration on hydrophobic membranes with pore size 0.03; 0.05; 0.08 mcm. Further, the extract is concentrated and purified from low-molecular weight impurities on a hydrophilic ultra filtration membrane. The extract then undergoes diafiltration with demineralised water on the same membrane. The end product is extracted via spray drying. |
Clatrate complex of cyclodextrine or arabinogalactane with 9-phenyl-symm-octahydroselenoxantene, its production method (versions), pharmaceutical composition and medication / 2451680 Invention relates to a new clatrate complex of α-, β-, γ- or hydroxipropyl-β-cyclodextrine or arabinogalactane with 9-phenyl-symm-octahydroselenoxantene with formula (1) possibly - in α-crystalline form. The weight ratio of 9-phenyl-symm-octahydroselenoxantene to Cyclodextrine is from 1:3 to 1:30 or the weight ratio of 9-phenyl-symm-octahydroselenoxantene to arabinogalactane is from 1:10 to 1:20. Additionally proposed are a liquid phase method of the complex production, a solid phase method of the complex production, a pharmaceutical composition and a medication. |
Method of determining antibacterial activity of chitosan / 2450022 Disclosed is a method of determining antibacterial properties of chitosan by estimating its minimum bacteriostatic and/or bactericidal concentration. Complex buffer solutions based on three organic acids MES, ACES and TES with different pH values are prepared. The ready buffer solutions are poured into a vessel. Double dilutions of chitosan are then prepared in vessels with the buffer solutions. Aliquots of a bacterial suspension in a fluid medium are added to the chitosan solutions in the buffer. The solutions are incubated for 24 hours at temperature which is optimum for bacterial growth. The minimum bacteriostatic and/or minimum bactericidal concentration of chitosan is then determined after incubation by determining growth of the culture or a drop in the number of living cells, respectively. |
Antigenic preparations / 2245721 Antigenic preparations are obtained from keratinophilic fungi Trichophiton or Microsporum species or yeast species Candida by alkali hydrolysis techniques. Thus obtained preparations can be, in particular used, as vaccines and for treating allergy and modulating immune response. |
Method for preparing exopolysaccharides / 2246540 Invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture. |
Method for producing of chitosan from chitin of cancerous / 2246880 Method involves providing deacetylation of raw material with the use of preliminarily cooled alkaline solution; washing and drying. Deacetylation process is performed in three stages, first stage being performed for 7 days and subsequent two stages being performed for 2 hours each, combined with thermal processing at temperature of 55-590C. Washing process is provided after each deacetylation stage. |
Method for preparing pectin from citrus husks / 2247730 Invention relates to a method for isolating pectin from vegetable raw. Method for preparing pectin from citrus husks involves hydrolysis-extraction of a solid phase and separation of a liquid phase. Before hydrolysis-extraction a solid phase is extracted with ethyl acetate and liquid acetylene successively under the above atmosphere pressure. The second extract is separated without pressure change followed by abrupt dropping pressure over a solid phase up to atmosphere value and feeding a solid phase to hydrolysis-extraction. Invention provides reducing loss of pectin being without deterioration of its organoleptic properties. |
Method for isolating pectin from sunflower heads / 2247731 Invention relates to a method for isolating pectin from the vegetable raw. Method for isolating pectin from sunflower heads involves their milling, denaturation of proteins by heating, hydrolysis-extraction of solid phase, separation of liquid phase and its concentrating and drying. Extraction with dimethylformamide is carried out before hydrolysis-extraction stage followed by extraction with liquid carbon dioxide under above atmosphere pressure. The second extract is separated without change of pressure followed by abrupt drop of pressure over the solid phase up to atmosphere value and feeding the solid phase to hydrolysis-extraction stage. Invention provides improving safety due to exclusion contact of toxic solvent with the end product, reducing time process of hydrolysis-extraction stage and reducing loss of pectin substances. |
Method for preparing pectin from citrus husks / 2247732 Invention relates to a method for preparing pectin from vegetable raw. Method for preparing pectin from citrus husks involves hydrolysis-extraction of a solid phase and separation of a liquid phase. Before hydrolysis-extraction a solid phase is extracted successively with acetone and mixture of liquid acetylene and carbon dioxide taken in the mass ratio acetylene : carbon dioxide from 3:7 to 7:3, respectively, under the above atmosphere pressure. The second extract is separated without the pressure change followed by abrupt dropping pressure over a solid phase to atmosphere value and feeding a solid phase to hydrolysis-extraction. Invention provides reducing loss of pectin being without deterioration of its organoleptic properties. |
Method for preparing pectin from citrus husks / 2247733 Invention relates to a method for isolating pectin from vegetable raw. Method for preparing pectin from citrus husks involves hydrolysis-extraction of a solid phase and separation of a liquid phase. Before hydrolysis-extraction a solid phase is extracted with dimethylformamide and liquid acetylene successively under the above atmosphere pressure. The second extract is separated without pressure change followed by abrupt dropping pressure over a solid phase up to atmosphere value and feeding a solid phase to hydrolysis-extraction. Invention provides reducing loss of pectin being without deterioration of its organoleptic properties. |
Method for preparing pectin from citrus husks / 2247734 Invention relates to a method for isolating pectin from vegetable raw. Method for isolating pectin from citrus husks involves hydrolysis-extraction of a solid phase and separation of a liquid phase. Before hydrolysis-extraction a solid phase is extracted with dimethylformamide and liquid carbon dioxide successively under the above atmosphere pressure. The second extract is separated without the pressure change followed by abrupt dropping pressure over a solid phase up to atmosphere value and feeding a solid phase to hydrolysis-extraction. Invention provides reducing loss of pectin being without deterioration of its organoleptic properties. |
Method for preparing pectin from citrus husks / 2247735 Invention relates to a method for isolating pectin from vegetable raw. Method for preparing pectin from citrus husks involves hydrolysis-extraction of a solid phase and separation of a liquid phase. Before hydrolysis-extraction a solid phase is extracted with acetone and liquid carbon dioxide successively under the above atmosphere pressure. The second extract is separated without pressure change followed by abrupt dropping pressure over a solid phase up to atmosphere value and feeding a solid phase to hydrolysis-extraction. Invention provides reducing loss of pectin being without deterioration of its organoleptic properties. |
Method for isolating pectin from citrus husks / 2247736 Invention relates to a method for preparing pectin extract from vegetable raw. Method involves the successive extraction of citrus husks with acetone. Then after separation of extract a solid phase is extracted with a mixture of liquid acetylene and carbon dioxide taken in mass ratio from 3:7 to 7:3. Then hydrolysis-extraction of a solid phase is carried out and a liquid phase is separated after termination of the hydrolysis-extraction process. Method provides reducing loss of pectin. |
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