Method for isolating pectin from sunflower heads

FIELD: vegetable raw technology.

SUBSTANCE: invention relates to a method for isolating pectin from the vegetable raw. Method for isolating pectin from sunflower heads involves their milling, denaturation of proteins by heating, hydrolysis-extraction of solid phase, separation of liquid phase and its concentrating and drying. Extraction with dimethylformamide is carried out before hydrolysis-extraction stage followed by extraction with liquid carbon dioxide under above atmosphere pressure. The second extract is separated without change of pressure followed by abrupt drop of pressure over the solid phase up to atmosphere value and feeding the solid phase to hydrolysis-extraction stage. Invention provides improving safety due to exclusion contact of toxic solvent with the end product, reducing time process of hydrolysis-extraction stage and reducing loss of pectin substances.

EFFECT: improved isolating method.

 

The invention relates to the process of extracting pectin from plant materials.

A known method of extraction of pectin from baskets sunflower for grinding, heating to denature proteins, extraction with acetone, hydrolysis-extraction solid phase, separating the liquid phase and its concentration or drying (Sable I. C. Biochemical rationale for the technology of pectin increased biological value of blossoms sunflower. Abstract of thesis. Ph.D. - Krasnodar: Kuban state agrarian University, 1997, p.8-21).

The disadvantages of this method are high pozarovzryvobezopasnost production and the transition of acetone in the target product.

The technical result of the invention is to improve safety, preventing toxic solvent in the target product, shortening the duration of the hydrolysis-extraction, thereby reducing the loss of pectin.

This result is achieved in that in the method of extraction of pectin from baskets sunflower for grinding, heating to denature proteins, extraction, hydrolysis-extraction solid phase, separating the liquid phase and its concentration or drying, according to the invention, the extraction is carried dimethylformamide, and then liquid carbon dioxide at a pressure above atmospheric is about, separating the second extract without changing the pressure, then the pressure over the solid phase sharply dropping to atmospheric with the direction of the solid phase hydrolysis-extraction.

The method is implemented as follows.

Baskets of sunflower crushed and heated to denature proteins, as in the closest analogue to enable long-term storage and ease of extraction of pectic substances. The thus prepared raw material is extracted with dimethylformamide and separate the extract by known methods (Bykov SF Theoretical and experimental basis for the creation of a brand new resource-saving technologies of production of vegetable oils. Abstract of thesis. Dr. - SPb.: VNIIG, p.21-26; Demchenko P.P. improvement of the technology of extraction of oil from sunflower seeds. Abstract of thesis. Ph.D. - L.: VNIIG, 1982, S. 6-14)containing high-molecular non-polar substances, as in the closest analogue, with a bitter taste. Then the solid phase is extracted with liquid carbon dioxide at a pressure above atmospheric, corresponding to the vapor pressure of the extractant at a temperature of extraction, for example at room, and separate the extract without changing the pressure (Kasyanov GI and other Processing of plant raw material liquefied and compressed gases): Agroniiteipp, 199, 40 C.). The remains of dimethylformamide pass into the extract, which prevents further passage of dimethylformamide according to the stages of the technological process and its penetration into the target product. Pressure over the solid phase sharply dropped to atmospheric. This leads to a sharp boiling liquefied gas and explosive grinding plant, accompanied by the destruction of cell membranes. Then the solid phase is directed to the hydrolysis-extraction.

It should be noted that pectic substances do not dissolve or in dimethylformamide or in carbon dioxide, which prevents their loss with detachable extracts and, accordingly, increases the yield of pectin compared with the closest analogue. Despite the fact that the initial content of pectin in baskets sunflower is from 12 to 24% in terms of dry substance, and the content of water-soluble fractions from 30 to 40% (Donchenko L. C. Technology of pectin and pectinibranchia): new Delhi, 2000, p.53, 55), loss of pectin as a result of his deep hydrolysis can be estimated for the proposed method and the closest analogue for known dependencies (Altonian M.K. Kinetics of degradation of the polysaccharide in the process of obtaining pectin. Abstract of thesis. Ph.D. - Krasnodar: KPI, 1988) and is 3-4% vs. 12-16% in the prototype.

Hydrolysis-extraction is carried out with couples who metres, selected by well-known recommendations (closest counterpart: Donchenko L. C. Technology of pectin and pectinibranchia): new Delhi, 2000, p.143-142) or calculated according to known dependencies (Ilyin I.A. Scientific technology of the modified pectin - Krasnodar: Scznyaw, 2001, p.88-156). The absence at this stage of the process volatile organic solvent eliminates the possibility of contact with acid and fire or explosion, which increases safety. At the same time due to the preliminary destruction of cell membranes increases the contact surface phases and availability protopectin substances for hydrolysis-extraction. This reduces the processing time and reduce the possibility of deep hydrolysis and loss of pectin. After completion of the hydrolysis-extraction of the separated liquid phase containing pectin, which concentrated or dried. The absence at this stage of the technological process volatile organic solvent eliminates the possibility of contact with the heating surfaces and ignition or explosion, as well as hitting the target product.

Thus, the proposed method can improve production safety, to prevent toxic solvent in the target product, reduce the duration of hydrolysis-ekstragirovanie and, thereby, reduce the loss of pectic substances.

Method of extraction of pectin from baskets sunflower for grinding, heating to denature proteins, hydrolysis-extraction solid phase, separating the liquid phase and its concentration or drying, characterized in that before hydrolysis-extraction carry out extraction with dimethylformamide, and then carry out the extraction with liquid carbon dioxide at a pressure above atmospheric, separating the second extract without changing the pressure, then the pressure over the solid phase sharply dropping to atmospheric with the direction of the solid phase hydrolysis-extraction.



 

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