Method of producing arabinogalactan. RU patent 2454429.

FIELD: chemistry.

SUBSTANCE: invention relates to methods of producing arabinogalactan. The method involves aqueous extraction of arabinogalactan from resin-free larchwood while heating. The obtained arabinogalactan extract is purified from high-molecular weight impurities by ultra filtration on hydrophobic membranes with pore size 0.03; 0.05; 0.08 mcm. Further, the extract is concentrated and purified from low-molecular weight impurities on a hydrophilic ultra filtration membrane. The extract then undergoes diafiltration with demineralised water on the same membrane. The end product is extracted via spray drying.

EFFECT: invention enables to obtain arabinogalactan with high degree of purity of up to 96,5-98,0%, cut duration of the purification process and reduce power consumption.

4 ex

IPC classes for russian patent Method of producing arabinogalactan. RU patent 2454429. (RU 2454429):

C08B37 - Preparation of polysaccharides not provided for in groups ; C08B0001000000-C08B0035000000; Derivatives thereof (cellulose D21)

Another patents in same IPC classes:

Clatrate complex of cyclodextrine or arabinogalactane with 9-phenyl-symm-octahydroselenoxantene, its production method (versions), pharmaceutical composition and medication / 2451680
Invention relates to a new clatrate complex of α-, β-, γ- or hydroxipropyl-β-cyclodextrine or arabinogalactane with 9-phenyl-symm-octahydroselenoxantene with formula (1) possibly - in α-crystalline form. The weight ratio of 9-phenyl-symm-octahydroselenoxantene to Cyclodextrine is from 1:3 to 1:30 or the weight ratio of 9-phenyl-symm-octahydroselenoxantene to arabinogalactane is from 1:10 to 1:20. Additionally proposed are a liquid phase method of the complex production, a solid phase method of the complex production, a pharmaceutical composition and a medication.

Method of determining antibacterial activity of chitosan / 2450022
Disclosed is a method of determining antibacterial properties of chitosan by estimating its minimum bacteriostatic and/or bactericidal concentration. Complex buffer solutions based on three organic acids MES, ACES and TES with different pH values are prepared. The ready buffer solutions are poured into a vessel. Double dilutions of chitosan are then prepared in vessels with the buffer solutions. Aliquots of a bacterial suspension in a fluid medium are added to the chitosan solutions in the buffer. The solutions are incubated for 24 hours at temperature which is optimum for bacterial growth. The minimum bacteriostatic and/or minimum bactericidal concentration of chitosan is then determined after incubation by determining growth of the culture or a drop in the number of living cells, respectively.

Kefiran production method / 2450021
Kefiran production method involves kefiran extraction from kefir fungi in boiling water, the produced solution cooling, kefiran precipitation with an equivalent quantity of ethanol, the solution maintenance, the product drying, extraction is performed (while stirring) in boiling water at a ratio of kefir fungi to water equal to approximately 1:10 during 5-10 minutes till homogeneous mass production. In the process of cooling one adds a proteolytic enzyme and maintains the product at a temperature and pH optimal for the proteolytic enzyme used during no less than 1 hour; the extract cooled to 20°C is filtered through a fine filter; after ethanol precipitation the sediment is filtered, washed with distilled water and repeatedly filtered several times.

Clathrate complexes of beta-cyclodextrin with 1-{[6-bromo-1-methyl-5-methoxy-2-phenylthiomethyl-1-h-indol-3-yl]carbonyl}-4-benzylpiperazine, having antiviral activity, synthesis and use thereof / 2448120
Invention relates to a novel clathrate complex of β-cyclodextrin with 1-{[6-bromo-1-methyl-5-methoxy-2-phehylthiomethyl-1-H-indol-3-yl]carbonyl}-4-benzylpiperazine of formula : with molar ratio 1-{[6-bromo-1-methyl-5-methoxy-2-phehylthiomethyl-1-H-indol-3-yl]carbonyl}-4-benzylpiperazine: β-cyclodextrin from 1:1 to 1:10, synthesis method and use thereof as an antiviral agent for treating influenza. The disclosed method involves mixing solutions of β-cyclodextrin and 1-{[6-bromo-1-methyl-5-methoxy-2-phehylthiomethyl-1-H-indol-3-yl]carbonyl}-4-benzylpiperazine in molar ratio from 1:1 to 1:10 while stirring and heating to temperature not higher than 70°C and then maintaining said conditions until a homogeneous solution is obtained and extraction of the obtained complex.

Method for production of polysaccharides of coniferous trees greens / 2448119
Method envisages milling coniferous trees greens. Then one extracts the tree greens with ethyl acetate and chloroform. Then remains are extracted in three stages: with distilled water, with water acidified with hydrochloric acid, with water solution of potassium hydroxide. Then one proceeds with concentration and settlement with ethyl alcohol. Then the sediment is centrifuged, dissolved in water and dialysed against distilled water. One performs lyophilic drying of the target product.

Method of producing water-soluble forms of aminomethyl terpenophenols / 2448085
Invention relates to a novel method of producing water-soluble forms of aminomethyl terpenophenols through a salt formation reaction of amines selected from a group of aminomethyl terpenophenols in 70% aqueous solution of an aliphatic alcohol, such as methanol, ethanol, propanol, isopropanol, or in a heterogeneous reaction medium consisting of water and a water-immiscible solvent, such as toluene, petroleum ether, diethyl ether, heated to temperature 30-70°C, with carboxyl-containing polysaccharides from a group of pectins, sulphated pectin derivatives, sulphated carboxymethylcellulose derivatives with aminomethyl terpenophenol content of 0.05-0.3 mol per free carboxyl group of polysaccharide while stirring for the process time of 60-240 minutes at temperature 15-25°C, with extraction of the obtained product.

Method of producing high-purity arabinogalactan / 2447086
Invention relates to methods of producing arabinogalactan. The method involves aqueous extraction of arabinogalactan from resin-free larchwood while heating. The obtained extract is concentrated and subjected to ultrafiltration. Ultrafiltration is carried out using coarse-pored cellulose acetate membranes UAM-500P. The obtained concentrate is treated with aqueous solution of a cationic flocculant. The clarified concentrated is treated with aqueous hydrogen peroxide solution in amount of 0.1-0.5 mol/l concentrate in the presence of a complexing agent in amount of 0.12-5.0 mmol/l. The ready product is extracted by spray drying.

Method for preparing boron-containing hyaluronic acid / 2445978
Invention refers to a method for preparing sodium salt of hyaluronic acid modified by boron compounds with no fluid medium added. The method consists in the fact that powdered sodium salt of hyaluronic acid together with a modifying agent and mixed modifying agents is pre-homogenised in a mixer at temperature ranging within 20° to 50°C; thereafter the prepared homogenous powder mixture is simultaneously exposed to pressure and shearing deformation in a mechanochemical reactor at temperature ranging within 20° to 50°C and pressure 5-1000 MPa.

Method of purifying non-protein fasciola antigens / 2445321
Method involves fractionation of a fasciola protein extract using chromatographic techniques. The pool of fractions in which non-protein antigens have been detected is treated with trichloroacetic acid. High pressure chromatography on an HR 10/30 column with superose 6 in an FPLC chromatographic system is used.

Method for producing chitosan oligomers / 2445101
Invention refers to medicine, more specifically to producing chitosan oligomers possessing biological activity and applicable in food industry and medicine. In a method for producing chitosan oligomers, a chitosan solution is taken in the concentration of 0.025-0.075% (weight/volume) and exposed to low-frequency ultrasound of the intensity of 92-460 Wt/cm2 for 5-30 minutes.

Antigenic preparations / 2245721
Antigenic preparations are obtained from keratinophilic fungi Trichophiton or Microsporum species or yeast species Candida by alkali hydrolysis techniques. Thus obtained preparations can be, in particular used, as vaccines and for treating allergy and modulating immune response.

Method for preparing exopolysaccharides / 2246540
Invention proposes a method for preparing exopolysaccharide by culturing microorganisms in nutrient medium containing one or more carbon source assimilated by microorganisms and caruba seeds fraction as nitrogen organic source. Applying the proposed method provides preparing exopolysaccharide eliciting improved organoleptic, sensor and visual properties. Invention can be used in building, paper, textile, cosmetic, food, oil output industry and agriculture.

Method for producing of chitosan from chitin of cancerous / 2246880
Method involves providing deacetylation of raw material with the use of preliminarily cooled alkaline solution; washing and drying. Deacetylation process is performed in three stages, first stage being performed for 7 days and subsequent two stages being performed for 2 hours each, combined with thermal processing at temperature of 55-590C. Washing process is provided after each deacetylation stage.

Method for preparing pectin from citrus husks / 2247730
Invention relates to a method for isolating pectin from vegetable raw. Method for preparing pectin from citrus husks involves hydrolysis-extraction of a solid phase and separation of a liquid phase. Before hydrolysis-extraction a solid phase is extracted with ethyl acetate and liquid acetylene successively under the above atmosphere pressure. The second extract is separated without pressure change followed by abrupt dropping pressure over a solid phase up to atmosphere value and feeding a solid phase to hydrolysis-extraction. Invention provides reducing loss of pectin being without deterioration of its organoleptic properties.

Method for isolating pectin from sunflower heads / 2247731
Invention relates to a method for isolating pectin from the vegetable raw. Method for isolating pectin from sunflower heads involves their milling, denaturation of proteins by heating, hydrolysis-extraction of solid phase, separation of liquid phase and its concentrating and drying. Extraction with dimethylformamide is carried out before hydrolysis-extraction stage followed by extraction with liquid carbon dioxide under above atmosphere pressure. The second extract is separated without change of pressure followed by abrupt drop of pressure over the solid phase up to atmosphere value and feeding the solid phase to hydrolysis-extraction stage. Invention provides improving safety due to exclusion contact of toxic solvent with the end product, reducing time process of hydrolysis-extraction stage and reducing loss of pectin substances.

Method for preparing pectin from citrus husks / 2247732
Invention relates to a method for preparing pectin from vegetable raw. Method for preparing pectin from citrus husks involves hydrolysis-extraction of a solid phase and separation of a liquid phase. Before hydrolysis-extraction a solid phase is extracted successively with acetone and mixture of liquid acetylene and carbon dioxide taken in the mass ratio acetylene : carbon dioxide from 3:7 to 7:3, respectively, under the above atmosphere pressure. The second extract is separated without the pressure change followed by abrupt dropping pressure over a solid phase to atmosphere value and feeding a solid phase to hydrolysis-extraction. Invention provides reducing loss of pectin being without deterioration of its organoleptic properties.

Method for preparing pectin from citrus husks / 2247733
Invention relates to a method for isolating pectin from vegetable raw. Method for preparing pectin from citrus husks involves hydrolysis-extraction of a solid phase and separation of a liquid phase. Before hydrolysis-extraction a solid phase is extracted with dimethylformamide and liquid acetylene successively under the above atmosphere pressure. The second extract is separated without pressure change followed by abrupt dropping pressure over a solid phase up to atmosphere value and feeding a solid phase to hydrolysis-extraction. Invention provides reducing loss of pectin being without deterioration of its organoleptic properties.

Method for preparing pectin from citrus husks / 2247734
Invention relates to a method for isolating pectin from vegetable raw. Method for isolating pectin from citrus husks involves hydrolysis-extraction of a solid phase and separation of a liquid phase. Before hydrolysis-extraction a solid phase is extracted with dimethylformamide and liquid carbon dioxide successively under the above atmosphere pressure. The second extract is separated without the pressure change followed by abrupt dropping pressure over a solid phase up to atmosphere value and feeding a solid phase to hydrolysis-extraction. Invention provides reducing loss of pectin being without deterioration of its organoleptic properties.

Method for preparing pectin from citrus husks / 2247735
Invention relates to a method for isolating pectin from vegetable raw. Method for preparing pectin from citrus husks involves hydrolysis-extraction of a solid phase and separation of a liquid phase. Before hydrolysis-extraction a solid phase is extracted with acetone and liquid carbon dioxide successively under the above atmosphere pressure. The second extract is separated without pressure change followed by abrupt dropping pressure over a solid phase up to atmosphere value and feeding a solid phase to hydrolysis-extraction. Invention provides reducing loss of pectin being without deterioration of its organoleptic properties.

Method for isolating pectin from citrus husks / 2247736
Invention relates to a method for preparing pectin extract from vegetable raw. Method involves the successive extraction of citrus husks with acetone. Then after separation of extract a solid phase is extracted with a mixture of liquid acetylene and carbon dioxide taken in mass ratio from 3:7 to 7:3. Then hydrolysis-extraction of a solid phase is carried out and a liquid phase is separated after termination of the hydrolysis-extraction process. Method provides reducing loss of pectin.