|
RussianPatents.com
|
Antigenic preparations. RU patent 2245721. |
|
            
|
FIELD: immunotherapeutic agents. SUBSTANCE: antigenic preparations are obtained from keratinophilic fungi Trichophiton or Microsporum species or yeast species Candida by alkali hydrolysis techniques. Thus obtained preparations can be, in particular used, as vaccines and for treating allergy and modulating immune response. EFFECT: expanded immunotherapeutic possibilities. 17 cl, 5 dwg, 12 tbl, 20 ex
|
 Method for differential diagnosis of representatives of family chlamydiaceae / 2245370Invention proposes a new method for differential diagnosis of representatives of family Chlamydiaceae. Method involves isolation of DNA from pathogen, amplification of target using primers CM1 and CM2 showing specificity to 5'-terminal fragment of gene omp1 and electrophoretic separation of polymerase chain reaction (PCR) products. Electrophoresis is carried out in agarose gel with addition of sequence-specific DNA-ligand - bis-benzimide-PEG. The species belonging of PCR-products is determined by comparison of the migration rate of PCR-products in gel with electrophoretic mobility of control. Proposed method provides carrying out the differentiation of all species of family Chlamydiaceae and method is simple and rapid and can be used for direct diagnosis of clinical material samples also. Invention can be used in medicine and virology for differential diagnosis of representatives of family Chlamydiaceae. |
| Method for differential diagnosis of representatives of family chlamydiaceae / 2245369 Invention proposes a new method for differential diagnosis of representatives of family Chlamydiaceae. Method involves isolation of DNA of pathogen, amplification using real-time polymerase chain reaction (PCR) and primers CM1 and CM2 exhibiting specificity to 5'-terminal fragment of gene omp1 followed by post-amplification analysis of curves of PCR-products melting in the presence of nonspecific fluorescent dye SYBR Green I for separation of Chlamydia species and electrophoretic separation of PCR-products. Identification of species is carried out on the basis of differences in PCR-products melting point wherein melting point curves of all fragments of omp1 are characterized by the presence of two peaks reflecting two-stage dissociation of DNA chains in sites with different A/T-saturation degree. Proposed method provides carrying out the differentiation of all species of Chlamydia that are pathogenic for humans. Except for, method provides carrying out the differentiation of Chlamydiaceae causing diseases in animals and also method is simple, rapid and can be used for direct diagnosis of clinical material samples. Invention can be used in medicine, veterinary science and virology for differential diagnosis of representatives of family Chlamydia. |
| Method for indication of microorganisms / 2245368 Method involves sampling of specimen from environment and addition of bacteriophage to sample that is able to multiplicate in analyzed microorganism. Inoculate is kept for a definite time and then bacteriophage titer is determined. The presence of microorganism is proved by increase the bacteriophage titer value measuring by degree of light diffusion in sample. Except for, in increase of light diffusion the sample is contacted with either immunosorbent and radioactivity of immunosorbent is determined or with piezoelectric resonator with electrodes covered by an antiphage Ig and the presence of piezoeffect is assayed. Invention can be used in carrying out analysis for the presence of microorganisms in air and liquid medium, monitoring of environment in periodic regime being in closed compartments mainly and in places with human mass. |
 Method for biotesting water and aqueous extract samples / 2245367Invention is designated for biotesting water and aqueous extract samples. Daphnia are immersed firstly in solution or water for culturing to be tested and then daphnia are transferred into lightproof chamber with outlet hole for culturing and this chamber is immersed into water. Time passing out daphnia from the testing chamber to light is measured and toxicity of analyzed solution is estimated by difference time in daphnia passing out. Method allows carrying out the rapid control of water and aqueous extracts toxicity in laboratory and field conditions. |
| Strain of mycelial fungus aspergillus awamori as producer of glucoamylase / 2245364 The strain Aspergillus awamori VKM F-3771 D is obtained by the multiple-stage genetic selection of the parent strain Aspergillus awamori VUD T-2 F-203 using ultraviolet irradiation. The strain elicits high activity of enzyme glucoamylase. For providing the high productivity of the strain nutrient media can be used that are used usually in industrial technologies in preparing similar enzyme preparations being without using complex procedures in culturing the strain-producer. Invention can be used for saccharification of starchy raw in different branches of food industry wherein highly active enzyme preparations with resistance to acid pH values are required. |
| Avirulent strain of microorganism vibrio cholerae km 169 of serogroup o139 as producer of capsule antigen / 2245363 Avirulent strain as a producer of capsule antigen is obtained on the base of the natural strain V. cholerae of serogroup O139 by method of step-by-step selection of cells with enhanced production of capsule. Prepared strain forms antigen by 3-4-fold more as compared with other strains of this serogroup. The strain shows high and stable level of capsule antigen production that is one of components of chemical choleraic vaccine against cholera pathogen of serogroup O139. |
 Method for intensifying number of microorganisms of genus pseudomonas in growing cereal crops / 2244746Invention relates to a method for intensifying growth of microorganisms of genus Pseudomonas in growing cereal crops. Method involves presowing treatment of gramineous crops seeds with agent solution representing an aqueous solution of triple copolymer of acrylic acid, its amide and triacryloylhexahydro-1,3,5-triazine in the following ratio of components, wt.-%: active component, 0.0005-0.001; water, the balance. Method provides elevating number of microorganisms of genus Pseudomonas in rhizosphere and rhizoplane in rice and wheat roots. |
| Bacterial preparation, method for its producing, nutrient medium for culturing cells escherichia coli vkm cr-322d and method for prophylaxis and treatment of gastroenteric disease in agricultural and domestic animal and poultry / 2244743 For preparing a preparation cells of microorganism Escherichia coli VKM CR-322D is cultured in nutrient medium containing Hottinger's broth, glucose, yeast extract, manganese sulfate, potassium hydrophosphate, sodium chloride and tap water in the content of amine nitrogen 125-155 mg%. Glucose is added by batch portions in the process of culturing cells that is carried out at temperature 30-31oC at stirring and aeration for 10-12 h. Prepared cultural fluid containing 3 x 109 bacterial cells/ml is mixed with protective sucrose-gelatin medium and subjected for lyophilic drying. Dried mass is stored under nitrogen that enhances safety of viable cells in the preparation. Applying the preparation for prophylaxis and treatment of agricultural and domestic animals and poultries with gastroenteric diseases provides its effectiveness. |
| Method for isolation and selection of microorganisms as producers of cyclodextrin glucanotrasnferase, strain of microorganism bacillus circulans b-65 ncaim (p) 001277 (b-65) as producer of extracellular cyclodextrin transferase, cyclodextrin glucanotransferase obtained from its and its applying for preparing cyclodextrin / 2244742 The strain Bacillus circulans B-65 a producer of cyclodextrin glucanotransferase is isolated and selected form the soil sample by culturing in nutrient medium with amylolytic activity 12.17 U/ml and cyclodextrinogenic activity up to 0.530 U/ml. Cyclodextrin glucanotransferase isolated from B. circulans B-65 shows the high degree for conversion of starch to cyclodextrins and this enzyme is specific for formation of β-cyclodextrin. Invention can be used in food industry for preparing cyclodextrins and cyclodextrin glucanotransferase used in different branches of industry. |
 Method for production of protheolytic reagent for structural protein hydrolysis / 2244741Method for production of protheolytic reagent includes cultivation of producer strain selected from dermatophyte of species Trichophyton mentagrophytes, Trichophyton verrucosum, Trichophyton equinum, Microsporum canis, Microsporum gypseum, preferably from fungus strains capable to produce complex of structural protein proteinases (scleroproteases) with total protheolytic activity at least 0.7 U/mg including collagenase 0.1-4.5 U/mg; keratinase 0.1-0.5- U/mg; elastase 0.5-1.9 U/mg. Cultivation is carried out, for example, in wort agar-agar or in wort broth for 20-24 days. |
| Method for differential diagnosis of representatives of family chlamydiaceae / 2245370 Invention proposes a new method for differential diagnosis of representatives of family Chlamydiaceae. Method involves isolation of DNA from pathogen, amplification of target using primers CM1 and CM2 showing specificity to 5'-terminal fragment of gene omp1 and electrophoretic separation of polymerase chain reaction (PCR) products. Electrophoresis is carried out in agarose gel with addition of sequence-specific DNA-ligand - bis-benzimide-PEG. The species belonging of PCR-products is determined by comparison of the migration rate of PCR-products in gel with electrophoretic mobility of control. Proposed method provides carrying out the differentiation of all species of family Chlamydiaceae and method is simple and rapid and can be used for direct diagnosis of clinical material samples also. Invention can be used in medicine and virology for differential diagnosis of representatives of family Chlamydiaceae. |
| Method for differential diagnosis of representatives of family chlamydiaceae / 2245369 Invention proposes a new method for differential diagnosis of representatives of family Chlamydiaceae. Method involves isolation of DNA of pathogen, amplification using real-time polymerase chain reaction (PCR) and primers CM1 and CM2 exhibiting specificity to 5'-terminal fragment of gene omp1 followed by post-amplification analysis of curves of PCR-products melting in the presence of nonspecific fluorescent dye SYBR Green I for separation of Chlamydia species and electrophoretic separation of PCR-products. Identification of species is carried out on the basis of differences in PCR-products melting point wherein melting point curves of all fragments of omp1 are characterized by the presence of two peaks reflecting two-stage dissociation of DNA chains in sites with different A/T-saturation degree. Proposed method provides carrying out the differentiation of all species of Chlamydia that are pathogenic for humans. Except for, method provides carrying out the differentiation of Chlamydiaceae causing diseases in animals and also method is simple, rapid and can be used for direct diagnosis of clinical material samples. Invention can be used in medicine, veterinary science and virology for differential diagnosis of representatives of family Chlamydia. |
| Method for indication of microorganisms / 2245368 Method involves sampling of specimen from environment and addition of bacteriophage to sample that is able to multiplicate in analyzed microorganism. Inoculate is kept for a definite time and then bacteriophage titer is determined. The presence of microorganism is proved by increase the bacteriophage titer value measuring by degree of light diffusion in sample. Except for, in increase of light diffusion the sample is contacted with either immunosorbent and radioactivity of immunosorbent is determined or with piezoelectric resonator with electrodes covered by an antiphage Ig and the presence of piezoeffect is assayed. Invention can be used in carrying out analysis for the presence of microorganisms in air and liquid medium, monitoring of environment in periodic regime being in closed compartments mainly and in places with human mass. |
| Method for biotesting water and aqueous extract samples / 2245367 Invention is designated for biotesting water and aqueous extract samples. Daphnia are immersed firstly in solution or water for culturing to be tested and then daphnia are transferred into lightproof chamber with outlet hole for culturing and this chamber is immersed into water. Time passing out daphnia from the testing chamber to light is measured and toxicity of analyzed solution is estimated by difference time in daphnia passing out. Method allows carrying out the rapid control of water and aqueous extracts toxicity in laboratory and field conditions. |
| Strain of mycelial fungus aspergillus awamori as producer of glucoamylase / 2245364 The strain Aspergillus awamori VKM F-3771 D is obtained by the multiple-stage genetic selection of the parent strain Aspergillus awamori VUD T-2 F-203 using ultraviolet irradiation. The strain elicits high activity of enzyme glucoamylase. For providing the high productivity of the strain nutrient media can be used that are used usually in industrial technologies in preparing similar enzyme preparations being without using complex procedures in culturing the strain-producer. Invention can be used for saccharification of starchy raw in different branches of food industry wherein highly active enzyme preparations with resistance to acid pH values are required. |
| Avirulent strain of microorganism vibrio cholerae km 169 of serogroup o139 as producer of capsule antigen / 2245363 Avirulent strain as a producer of capsule antigen is obtained on the base of the natural strain V. cholerae of serogroup O139 by method of step-by-step selection of cells with enhanced production of capsule. Prepared strain forms antigen by 3-4-fold more as compared with other strains of this serogroup. The strain shows high and stable level of capsule antigen production that is one of components of chemical choleraic vaccine against cholera pathogen of serogroup O139. |
| Method for intensifying number of microorganisms of genus pseudomonas in growing cereal crops / 2244746 Invention relates to a method for intensifying growth of microorganisms of genus Pseudomonas in growing cereal crops. Method involves presowing treatment of gramineous crops seeds with agent solution representing an aqueous solution of triple copolymer of acrylic acid, its amide and triacryloylhexahydro-1,3,5-triazine in the following ratio of components, wt.-%: active component, 0.0005-0.001; water, the balance. Method provides elevating number of microorganisms of genus Pseudomonas in rhizosphere and rhizoplane in rice and wheat roots. |
| Bacterial preparation, method for its producing, nutrient medium for culturing cells escherichia coli vkm cr-322d and method for prophylaxis and treatment of gastroenteric disease in agricultural and domestic animal and poultry / 2244743 For preparing a preparation cells of microorganism Escherichia coli VKM CR-322D is cultured in nutrient medium containing Hottinger's broth, glucose, yeast extract, manganese sulfate, potassium hydrophosphate, sodium chloride and tap water in the content of amine nitrogen 125-155 mg%. Glucose is added by batch portions in the process of culturing cells that is carried out at temperature 30-31oC at stirring and aeration for 10-12 h. Prepared cultural fluid containing 3 x 109 bacterial cells/ml is mixed with protective sucrose-gelatin medium and subjected for lyophilic drying. Dried mass is stored under nitrogen that enhances safety of viable cells in the preparation. Applying the preparation for prophylaxis and treatment of agricultural and domestic animals and poultries with gastroenteric diseases provides its effectiveness. |
| Method for isolation and selection of microorganisms as producers of cyclodextrin glucanotrasnferase, strain of microorganism bacillus circulans b-65 ncaim (p) 001277 (b-65) as producer of extracellular cyclodextrin transferase, cyclodextrin glucanotransferase obtained from its and its applying for preparing cyclodextrin / 2244742 The strain Bacillus circulans B-65 a producer of cyclodextrin glucanotransferase is isolated and selected form the soil sample by culturing in nutrient medium with amylolytic activity 12.17 U/ml and cyclodextrinogenic activity up to 0.530 U/ml. Cyclodextrin glucanotransferase isolated from B. circulans B-65 shows the high degree for conversion of starch to cyclodextrins and this enzyme is specific for formation of β-cyclodextrin. Invention can be used in food industry for preparing cyclodextrins and cyclodextrin glucanotransferase used in different branches of industry. |
| Method for production of protheolytic reagent for structural protein hydrolysis / 2244741 Method for production of protheolytic reagent includes cultivation of producer strain selected from dermatophyte of species Trichophyton mentagrophytes, Trichophyton verrucosum, Trichophyton equinum, Microsporum canis, Microsporum gypseum, preferably from fungus strains capable to produce complex of structural protein proteinases (scleroproteases) with total protheolytic activity at least 0.7 U/mg including collagenase 0.1-4.5 U/mg; keratinase 0.1-0.5- U/mg; elastase 0.5-1.9 U/mg. Cultivation is carried out, for example, in wort agar-agar or in wort broth for 20-24 days. |
 Thrombopoietin / 2245365Invention describes homogenous polypeptide ligand mpI representing polypeptide fragment of the formula: X-hTPO-Y wherein hTPO has amino acid sequence of human fragments TPO (hML); X means a amino-terminal amino-group or amino acid(s) residue(s); Y means carboxy-terminal carboxy-group or amino acid(s) residue(s), or chimeric polypeptide, or polypeptide fragment comprising N-terminal residues of amino acid sequence hML. Also, invention relates to nucleic acid encoding polypeptide and expressing vector comprising nucleic acid. Invention describes methods for preparing the polypeptide using cell-host transformed with vector, and antibodies raised against to polypeptide. Invention describes methods and agents using active agents of this invention. The polypeptide ligand mpI effects on replication, differentiation or maturation of blood cells being especially on megacaryocytes and progenitor megacaryocyte cells that allows using polypeptides for treatment of thrombocytopenia. |
| © 2013-2014 Russian business network RussianPatents.com - Special Russian commercial information project for world wide. Foreign filing in English. |