Bioactive organomineral slow-release fertilizer |
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IPC classes for russian patent Bioactive organomineral slow-release fertilizer (RU 2323918):
Method for preparing halophilic bacterium biomass / 2323251
Method involves culturing halophilic microorganisms in the presence of encapsulated adsorbent and/or antioxidant up to the stationary growth phase in preparing the seeding material. Then in submerged culturing fresh medium is added by portions in the amount 1/3 of the final volume of cultural fluid followed by contacting cultural fluid with an encapsulated adsorbent and/or antioxidant, and additional feeding with dry or concentrated medium is carried out. The amount of dissolved oxygen is maintained at the level 5-10% of the equilibrium level. Invention provides increasing the level of accumulation of biomass, to enhance the specific content of bacteriorhodopsin in halophilic microorganisms biomass and the total yield of bacteriorhodopsin at the minimal content of carotinoids in biomass, and to reduce consumption of nutrient medium also.
Method for preparing halophilic bacterium biomass / 2323226
Method involves using an encapsulated adsorbent and/or antioxidant in preparing seeding material and submerged culturing. In preparing seeding material the culturing process is carried out in freshly prepared nutrient medium up to the stationary growth phase. Submerged culturing is carried out by simultaneous inoculation of bioreactor with seeding material and contacting the content of bioreactor with encapsulated adsorbent and/or antioxidant. The oxygen content in bioreactor is maintained at the level 5-10% of the equilibrium level. Invention provides increasing yield of halophilic microorganisms biomass up to 42 g/l and with the content of bacteriorhodopsin up to 1.6 g/l and in practically absent of carotinoids in biomass. Invention provides reducing time for preparing the unit mass of the end product and to decrease consumptions for production of bacteriorhodopsin.
Nutrient medium for culturing mycobacterium and nocardioformous actinomyces / 2322495
Invention proposes a nutrient medium comprising potassium hydrogen phosphate, magnesium sulfate, L-asparagine, glycerol, citric acid, ferrous ammonium citrate, agar, humivite and distilled water. Invention provides enhancing growth properties of the nutrient medium.
 Microorganism strain bacillus simplex as producer of site-specific endonuclease blsi / 2322494
Invention proposes the strain Bacillus simplex 23 isolated from soil and providing preparing site-specific endonuclease. This enzyme is able for recognizing and cleaving both chain in nucleotide sequence of DNA comprising at least one C5-methylcytosine base in the recognition site 5'-GCNGC-3' to form 3'-prominent ends. The novel strain can be used for isolation of the novel site-specific endonuclease that can be used for detection and cleavage of methylated sites in DNA.
Microorganism strain paracoccus denitrificans as producer of exopolysaccharide and exopolysaccharide / 2322493
Invention relates to a novel culture of microorganism producing high-molecular exopolysaccharide. Invention proposes the strain of microorganism Paracoccus denitrificans VKPM B-8617 that produces exopolysaccharide possessing cross-linking properties in aqueous and water-containing hydrocarbon systems. Exopolysaccharide is formed by residues of glucose, galactose, mannose and rhamnose in the ratio = 52:4:1, respectively, and comprises glucuronic and pyruvic acids, and acyl groups also and has molecular mass (0.5 x 106)-(2 x 107) Da. This exopolysaccharide is able to form pseudoplastic and thixotropic highly viscous solutions showing stable values of dynamic viscosity in the range of temperature from 20°C to 90°C and unstratifying emulsions. Proposed exopolysaccharide can be used in building, paper, textile, perfume-cosmetic, food, chemical, oil- and gas-extracting industry, agriculture, and in pharmaceutics and medicine.
 Glacial ice bacterium microorganism strain as producer of site-specific endonuclease glu i / 2322492
Strain of microorganism Glacial ice bacterium is isolated from soil and can be used for a preparing site-specific endonuclease that recognizes and cleaves both chain of methylated nucleotide sequence in DNA: 5'-G(m5C)↓NG(m5C)-3'. Use of the invention provides preparing the novel site-specific endonuclease showing specificity to a methylated sequence in DNA that can be used in DNA analysis.
Method of biologically processing bird dung / 2322427
Method envisages mixing bird dung with moisture-absorbing material followed by aerobic fermentation of the mixture in presence of microorganisms. Process is carried out at stirring until temperature of fermentation mixture spontaneously drops to 25-30°C, said microorganisms being consortium of strains: Bacillus subtilis B-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-9-7, Candida tropicalis Y-1520, Candida utilis Y-2441. Preferable implementation of the method is when above-listed strains are used in equal proportions and in amount of 1•108-1•109 cells in 1 mL per 1 ton bird dung.
Biopreparation for enhancing productivity of agricultural crops and improving quality of production / 2322061
Invention relates to biopreparations of effective microorganisms used in the plant growing. Proposed preparation consists of a mixture of suspension of the following strains deposited in VKPM: Agrobacterium tumefacients B-4116, Agrobacterium radiobacter B-956, Azotobacter chroococcum B-2375, Bacillus thuringiensis B-2918, Bacillus subtilis B-6554, Bacillus subtilis B-4419, Bacillus megaterium B-4440, Bacillus megaterium B-200, Bradyrhizobium japonicum B-1978, Ervinia ananas B-5292, Lactobacillus casei B-3961, Pseudomonas fluorescens B-1138, and Rhodopseudomonas palustris B-1629. Invention provides restoring soil and to improve its structure, increase germination of seeds, fortify immune system of plants, enhance resistance against diseases and pests that increases the yield significantly and high quality of the prepared foodstuffs. Proposed biopreparations can be used for autumn, spring and summer treatment of soil, roots and leaf feeding, and for presowing treatment of seeds.
 Composition for protection of vegetable cultures against fungal and bacterial diseases / 2322060
Invention proposes a composition containing chitosan, chitin, succinic and glutamic acids, heteroauxine, and strain Bacillus subtilis M-22 or strain Trichoderma lignorum T-36. The composition possesses antagonistic activity in combination with stimulating effect on growth of plants and provides the effective protection of vegetable cultures against fungal and bacterial diseases.
Microorganism strain klebsiella pneumoniae gisk №278 as producer of lysozyme inhibitor / 2321632
Invention relates to the microorganism strain Klebsiella pneumoniae GISK № 278 isolated from a patent feces suffering from intestine dysbacteriosis. The strain is used for preparing an agent for producing the lysozyme inhibitor. The level of activity of lysozyme inhibitor produced by this strain is 1.64-2.16 mcg/ml of *OD value.
Method of biologically processing bird dung / 2322427
Method envisages mixing bird dung with moisture-absorbing material followed by aerobic fermentation of the mixture in presence of microorganisms. Process is carried out at stirring until temperature of fermentation mixture spontaneously drops to 25-30°C, said microorganisms being consortium of strains: Bacillus subtilis B-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-9-7, Candida tropicalis Y-1520, Candida utilis Y-2441. Preferable implementation of the method is when above-listed strains are used in equal proportions and in amount of 1•108-1•109 cells in 1 mL per 1 ton bird dung.
 Method for production of complex microbiological fertilizer / 2318784
Claimed method includes combination of microbiological component with natural biocompatible carrier, wherein as one part of microbiological component inoculate material of arbuscular mycorrhiza fungi (AM fungi) is used, as natural biocompatible carrier defecate form sugar beet production is used and combination thereof takes place in rizosphere of mycorrhizing plants grown of defecate. Inoculate material of AM fungi is obtained by cultivation of mycorrhized agriculture plants, for example sorgo, Sudan grass, millet, etc. on defecate. Sorgo or other mycorrhized plant seeds are seeded in containers with defecate, pure or mixed culture of AM fungi is introduced therein in form of soil-root mixture in amount of 3-5 g per one seed at occurrence level of mycorrhized roots of at least 60 %. Plants are grown for 90-120 days, then roots with defecate are dried and ground to produce pellety mixture which is blended with adhesive. Then sorgo or other mycorrhized plant seeds are treated with obtained mixture, dried and seeded in defecate and are grown for 90-120 days. Further ground plant parts and upper 20 cm of defecate together with roots are cut, and rest parts of microbiological component such as aqueous suspension of nodule-forming bacteria and/or rizobacteria.
Method for treatment of alfalfa seeds / 2299188
Invention relates to methods used in treatment of alfalfa seeds. Method involves presowing treatment of alfalfa seeds with biopreparation based on the strain Sinorhizobium meliloti Yakutsky №1 GNU VNIIChM 1775 taken in the amount 1 billion of cells per 1 seeds of alfalfa. Method provides enhancing productivity of alfalfa and improving fertility of soils.
Actinomyces fradiae strain for reprocessing of organic waste from animal and poultry breeding / 2298031
Disclosed is Actinomyces fradiae-96 strain isolated from soil sampled form organic poultry breeding waste.
Method for production of peat-grains compost / 2296732
Claimed method includes blending of beer grains with peat in ratio of 1:1 or 1:2 followed bioactivator introducing and aerobic composting at 20-60°C and humidity of 65-70 % for 2-3 months. Compost ferment prepared by culturing of microorganism consortium, isolated from humified humous forest soil layers and containing fungi Aspergillus niger, Aspergillus oryzae, Aspergillus sydowii, Cephalosporium, Glyocladium Cda., Trichoderma sp., actinomycetes Streptomyces griseus, Streptomyces termoviolaceus, Streptomyces globisporus, Streptomyces rubber, Streptomyces viridosporus and bacteria Bacillus cereus, Bacillus mycoides, Bacillus sublilis, in amount of 15 % or more based on composing mixture mass on beer grains is used as bioactivator.
Method for production of fermented agriculture composition / 2296112
Claimed method includes microbial decomposition of plant waste in oxygen-enriched air forced circulation, wherein oxygen is obtained by Chlorella Vulgaris culturing. Carbon dioxide released in process of plant waste decomposition is collected and used in algae culturing. Method also includes manure deodorizing with calcium oxide or calcium hydroxide. Deodorized manure is mixed with mass obtained in process of plant waste decomposition. Mixture is fermented for 4-5 days and mixed with solid fraction of Chlorella Vulgaris. Said solid fraction of Chlorella Vulgaris in obtained by double freezing and defrosting of algae biomass and cell juice separation. Process is carried out in closed cycle. Obtained composition is useful both as fertilizer and feed supplement.
Biopreparation-petroleum destructor / 2292326
Invention relates to a biological preparation used in treatment of soil polluted with petroleum and oil products in simultaneous recovery of its physicochemical properties and natural biocenosis. The biopreparation-petroleum destructor comprises microorganism culture and bacterial fertilizer as fermented bedding avian dung containing the following native microorganisms: Clavibacter michiganese, Bacillus amyloliquefaciens and Micrococcus varians. As a microorganism culture the strain Bacillus cereus 3K is used with a titer value 1012 cells/g of bacterial fertilizer. Invention provides enhancing petroleum-destructing activity, to simulate development of petroleum-oxidizing microflora, to increase the rate of petroleum decomposition, to provide intensification of bacterial processes recovering agrochemical properties of soil, to carry out treatment of petroleum-polluted soils in regions with short heat period.
 Hyperthermophile strain caldothrix satsumae capable of fermentation of organic waste at high temperatures / 2291900
Strain Caldothrix satsumae YM081 is isolated from compost by fermentation of organic waste at 85°C or higher. Strain is deposited under number FERM BP-8233.
 Organic nitrogen-containing composition and fertilizer comprising thereof / 2291139
Invention proposes an organic nitrogen-containing composition comprising enzymatic mother solution prepared by culturing microorganism of genus Enterobacter that is able to produce L-glutamic acid in liquid medium. Culturing is carried out at pH value providing precipitation of L-glutamic acid under condition that L-glutamic acid is produced and accumulated with accompanying precipitation and the following separation of L-glutamic acid from medium. Microorganism can metabolize carbon source in liquid medium containing L-glutamic acid in the saturation concentration and carbon source at the definite pH value and possesses capacity to accumulate L-glutamic acid in the amount exceeding the saturating concentration of L-glutamic acid in liquid medium at this pH value. Prepared composition is used as a component of fertilizer. The claimed invention provides expanding assortment of fertilizers.
Method for compost production from organic waste / 2290390
Claimed method includes waste mixing with paunch mature as microelement source and baking or beer yeast or yeast extracts and autolysates. Mixture is moistened up to humidity of 30-50 % and composted under aerobic conditions, wherein carbon/nitrogen ratio in finished product is from 10 to 50.
Biological fertilizer / 2249584
Biological fertilizer contains (%): growth agents (e.g., aqueous extract from Kalanchoe leaves) 3; inhibitor of soil pest growth and fungal and bacterial diseases (e.g., soil-entomopatogenic associative nematodes and waste products thereof, as well as living nematode-symbiotic bacteria) 0.001 and 0.0001, respectively; feed source (e.g., sodium humate) 0.1; and balance: water. Fertilizer of present invention finds plants with feedstuff, physiologically active substances and reduces helpful symbiotic microflora of rhizosphere.
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FIELD: chemistry. SUBSTANCE: organomineral fertilizer, which contains rock phosphate (apatite), natural zeolite, oxidised brown coal in 1:0.15:2-1:0.2:5 ratio, is composted for 30-60 days, while exposed to phosphate-assimilating bacteria in amount of 105-106 cells/spores/ml, isolated from local soil using selection method. Content of labile phosphorus increases from 17.0 to 50.4 mg/kg of soil, and phosphatase activity in chestnut soil increases by 2.8-21.8 times. EFFECT: increase in yield of herbage oats. 3 ex
The invention relates to the field of agriculture, agricultural chemistry, in particular biologically active organo-mineral fertilizers containing phosphates (Apatite), natural zeolite, oxidized lignite and strain phosphate-mobilizing bacteria isolated from local soil. In Transbaikalia in connection with deep-frozen soil, and slow thawing, short and dry vegetation period to achieve increased productivity of agricultural crops is impossible without the use of fertilizers. However, high prices for industrial tuks, the poverty of their range and low paying capacity of the farmers reduce the amount of fertilizer application. Therefore, an urgent task is the creation of new types of fertilizers on the basis of local mineral and organic materials when using biotechnology. The favorable factor is the high security Transbaikalia, in particular the Republic of Buryatia, local mineral and organic raw materials. The phosphate-containing agronomy widely used as fertilizers (beetles GA, Gamzikov G.P. Prospects involving agrochemical resources in agriculture Siberia // Resources and problems of the use of agrochemical raw materials of Western Siberia. - Novosibirsk: Nauka, 1988. - P.30-39; Makarenkova R.P. agrochemical evaluation phospho who itov Siberian fields // Agrochemistry. - 1996. No. 4. - P.39-49). Soil Transbaikalia due to climatic features are characterized by low microbial activity, as a result, the phosphorus from mineral raw materials becomes inaccessible form, is solid fixation in the soil and the degree of assimilation does not exceed 20-25% (abasheeva N.E. Agrochemistry, soil Transbaikalia. - Novosibirsk: Science, SB RAS, 1992. - 214 S.; Ginzburg CE Phosphorus main soil types in the USSR. - M.: Nauka, 1981). One way of solving this problem is the use of soil microorganisms for absorption by plants of phosphorus from soil and mineral resources. The prototype of the invention is a complex fertilizer of prolonged action (EN 2186050 C1, CL S-1/100, C-057 11/02 from 27.07.2002. Bull. No. 21), the use of which increases yields of vegetable and forage crops, stimulates nitrification processes in marginal cryoarid soils Transbaikalia, but the efficiency of the actions, the intensification of biological and biochemical processes inferior to the proposed fertilizer. The technical result of the invention is to increase the biological activity of dry and seasonally frozen soils of Transbaikalia, the increase in the content of soil available phosphorus to 17.0 and 50.4 mg/kg of soil and yield of vegetable and fodder crops. The technical result is achieved by the fact that obrinje, including phosphates (Apatite), natural zeolite, oxidized lignite, crushed to a grain size of 0.25-0.20 mm, mixed in a ratio of 1:0.15 to:2-1:0,2:5 and compost under the influence of phosphate-mobilizing bacteria in the amount of 105-106cells/spores/ml, isolated from soil using selective sampling within 30-60 days at a moisture content of the mixture at the level of 65-70% of full capacity. The content of phosphate-mobilizing microorganisms was determined by the kinetics of formation of the rolling P2O5in the compost. During composting, microorganisms increases phosphorus to 17.0 and 50.4 mg/kg soil. Fosfataza activity in chestnut soils was increased 2.8-21,8 time. The invention is illustrated by the following examples of high biological activity and productivity cryoarid soil Transbaikalia. Example 1. Phosphates (Apatite), natural zeolite, oxidized lignite, crushed to a grain size of 0.25-0.20 mm, mixed in a ratio of 1:0.15 to:2 and compost under the influence of phosphate-mobilizing bacteria in the amount of 105cells/spores/ml, isolated from soil using selective sampling within 30 days when the humidity of the mixture at the level of 65-70% of full capacity. During composting, microorganisms increases phosphorus to 17.0 mg/kg soil, fosfates the I activity increased 2.8 times. Example 2. Phosphates (Apatite), natural zeolite, oxidized lignite, crushed to a grain size of 0.25-0.20 mm, mixed in a ratio of 1:0,17:3.5 and compost under the influence of phosphate-mobilizing bacteria in the amount of 105,5cells/spores/ml, isolated from soil by the method of selective screening within 45 days when the humidity of the mixture at the level of 67.5% of full capacity. During composting, microorganisms increases phosphorus to 33.7 mg/kg soil, fosfataza activity in chestnut soils grew at 11.8 times. Example 3. Phosphates (Apatite), natural zeolite, oxidized lignite, crushed to a grain size of 0.25-0.20 mm, mixed in a ratio of 1:0,2:5 and compost under the influence of phosphate-mobilizing bacteria in the amount of 106cells/spores/ml, isolated from soil using selective sampling, within 60 days when the humidity of the mixture at 70% of full capacity. During composting, microorganisms increases phosphorus to 50.4 mg/kg soil, fosfataza activity in chestnut soils increased to 21.8 times. The use of biologically active organo-mineral fertilizers prolonged action allows to increase the yield of oats for green mass in the first year at 25-32%, posledeistvii in the second year of 35-49%. Biological and active organic fertilizer of prolonged action, including phosphates (Apatite), natural zeolite, oxidized lignite, characterized in that the comminuted to a size of 0.25-0.20 mm phosphates (Apatite), natural zeolite, oxidized brown coal are mixed in a ratio of 1:0.15 to:2-1:0,2:5, composted under the influence of phosphate-mobilizing bacteria in the amount of 105-106cells/spores/ml, isolated from soil using selective sampling within 30-60 days at a moisture content of the mixture at the level of 65-70% of full capacity.
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