Method for production of complex microbiological fertilizer |
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IPC classes for russian patent Method for production of complex microbiological fertilizer (RU 2318784):
                                                                             
Method for treatment of alfalfa seeds / 2299188
Invention relates to methods used in treatment of alfalfa seeds. Method involves presowing treatment of alfalfa seeds with biopreparation based on the strain Sinorhizobium meliloti Yakutsky №1 GNU VNIIChM 1775 taken in the amount 1 billion of cells per 1 seeds of alfalfa. Method provides enhancing productivity of alfalfa and improving fertility of soils.
Actinomyces fradiae strain for reprocessing of organic waste from animal and poultry breeding / 2298031
Disclosed is Actinomyces fradiae-96 strain isolated from soil sampled form organic poultry breeding waste.
Method for production of peat-grains compost / 2296732
Claimed method includes blending of beer grains with peat in ratio of 1:1 or 1:2 followed bioactivator introducing and aerobic composting at 20-60°C and humidity of 65-70 % for 2-3 months. Compost ferment prepared by culturing of microorganism consortium, isolated from humified humous forest soil layers and containing fungi Aspergillus niger, Aspergillus oryzae, Aspergillus sydowii, Cephalosporium, Glyocladium Cda., Trichoderma sp., actinomycetes Streptomyces griseus, Streptomyces termoviolaceus, Streptomyces globisporus, Streptomyces rubber, Streptomyces viridosporus and bacteria Bacillus cereus, Bacillus mycoides, Bacillus sublilis, in amount of 15 % or more based on composing mixture mass on beer grains is used as bioactivator.
Method for production of fermented agriculture composition / 2296112
Claimed method includes microbial decomposition of plant waste in oxygen-enriched air forced circulation, wherein oxygen is obtained by Chlorella Vulgaris culturing. Carbon dioxide released in process of plant waste decomposition is collected and used in algae culturing. Method also includes manure deodorizing with calcium oxide or calcium hydroxide. Deodorized manure is mixed with mass obtained in process of plant waste decomposition. Mixture is fermented for 4-5 days and mixed with solid fraction of Chlorella Vulgaris. Said solid fraction of Chlorella Vulgaris in obtained by double freezing and defrosting of algae biomass and cell juice separation. Process is carried out in closed cycle. Obtained composition is useful both as fertilizer and feed supplement.
Biopreparation-petroleum destructor / 2292326
Invention relates to a biological preparation used in treatment of soil polluted with petroleum and oil products in simultaneous recovery of its physicochemical properties and natural biocenosis. The biopreparation-petroleum destructor comprises microorganism culture and bacterial fertilizer as fermented bedding avian dung containing the following native microorganisms: Clavibacter michiganese, Bacillus amyloliquefaciens and Micrococcus varians. As a microorganism culture the strain Bacillus cereus 3K is used with a titer value 1012 cells/g of bacterial fertilizer. Invention provides enhancing petroleum-destructing activity, to simulate development of petroleum-oxidizing microflora, to increase the rate of petroleum decomposition, to provide intensification of bacterial processes recovering agrochemical properties of soil, to carry out treatment of petroleum-polluted soils in regions with short heat period.
 Hyperthermophile strain caldothrix satsumae capable of fermentation of organic waste at high temperatures / 2291900
Strain Caldothrix satsumae YM081 is isolated from compost by fermentation of organic waste at 85°C or higher. Strain is deposited under number FERM BP-8233.
 Organic nitrogen-containing composition and fertilizer comprising thereof / 2291139
Invention proposes an organic nitrogen-containing composition comprising enzymatic mother solution prepared by culturing microorganism of genus Enterobacter that is able to produce L-glutamic acid in liquid medium. Culturing is carried out at pH value providing precipitation of L-glutamic acid under condition that L-glutamic acid is produced and accumulated with accompanying precipitation and the following separation of L-glutamic acid from medium. Microorganism can metabolize carbon source in liquid medium containing L-glutamic acid in the saturation concentration and carbon source at the definite pH value and possesses capacity to accumulate L-glutamic acid in the amount exceeding the saturating concentration of L-glutamic acid in liquid medium at this pH value. Prepared composition is used as a component of fertilizer. The claimed invention provides expanding assortment of fertilizers.
Method for compost production from organic waste / 2290390
Claimed method includes waste mixing with paunch mature as microelement source and baking or beer yeast or yeast extracts and autolysates. Mixture is moistened up to humidity of 30-50 % and composted under aerobic conditions, wherein carbon/nitrogen ratio in finished product is from 10 to 50.
Method for reprocessing of organic waste by composting with worms / 2290389
Claimed method includes preliminary composting of organic waste by introducing of paunch mature as microelement source and yeast or yeast extracts as nitrogen source. Organic waste with additives is agitated for 7-12 days to produce unripe compost. Unripe compost is mixed with waste from pulp-and paper or wood processing industry and additional nitrogen sources, molasses and earthworms. In process of composting with worms enzyme activity of composting mixture is controlled.
Method for preparing biohumus enriched with phosphorolytic microorganisms / 2286983
Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Agricultural animal manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting process is carried out for 2-3 months at temperature 16-32°C under natural conditions, and after separation of worms from biohumus microorganisms of species Bacillus megaterium var. phosphaticum are added to biohumus in the concentration 1010-1012 cells per 1 kg of biohumus. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity, decreasing material consumptions and decreasing time for preparing the preparation.
Biological fertilizer / 2249584
Biological fertilizer contains (%): growth agents (e.g., aqueous extract from Kalanchoe leaves) 3; inhibitor of soil pest growth and fungal and bacterial diseases (e.g., soil-entomopatogenic associative nematodes and waste products thereof, as well as living nematode-symbiotic bacteria) 0.001 and 0.0001, respectively; feed source (e.g., sodium humate) 0.1; and balance: water. Fertilizer of present invention finds plants with feedstuff, physiologically active substances and reduces helpful symbiotic microflora of rhizosphere.
Method for producing biologically active phosphorus-zeolite fertilizer with prolonged effect / 2255922
Invention elates to methods for producing biologically active phosphorus-zeolite fertilizers containing phosphorus, potassium, calcium, magnesium, sulfur, silicon, trace elements and phosphate-mobilizing microorganisms. Method for producing biologically active phosphorus-zeolite fertilizer involves mixing phosphorites with mordenite-containing tuff using phosphate-mobilizing microorganisms adsorbed on tuff from suspension and phosphorites with fraction size below 0.5 mm are mixed with mordenite-containing tuff with fraction size below 2 mm saturated with phosphate-mobilizing microorganisms in the mass ratio = 1:(0.05-0.1). The prepared fertilizer shows the following composite, wt.-%: P2O5, 2.7-3.5; K2O, 3.5-4.6; sulfur (S), 0.07-0.1; CaO, 6.1-6.8; MgO, 0.2-0.5; SiO2, 60.0-65.0; manganese (Mn), 1.44-2.77; zinc (Zn), 0.017-0.020; copper (Cu), 0.016-0.018; cobalt (Co), 0.0009-0.0013; molybdenum (Mo), 0.002-0.005, and phosphate-mobilizing microorganisms, 107-8 cells/spores/ Using the biologically active phosphorus-zeolite fertilizer in the dose 5 t per hectare increases amount of phosphate-mobilizing microorganisms in chestnut soil by 6-23 times, phosphatase activity by 7-24 times and enhances the content of nitrate nitrogen, mobile phosphorus and exchangeable potassium also.
Method for biological activation of natural zeolite / 2255926
Invention relates to biologically active zeolite fertilizers containing potassium, phosphorus, calcium, trace elements and phosphate-mobilizing microorganisms. Method for biological activation of mordenite-containing tuff with fraction size below 2 mm involves its mixing in rocker for 2-4 h with an aqueous suspension of phosphate-mobilizing microorganisms containing 109 cells/spores in the mass ratio = 1:(0.2-0.6) followed by keeping at temperature 4°C for 12 h and following drying up to the friable state. The prepared fertilizer consists of mordenite-containing tuff with fraction size below 2 mm and (4.5-8) x 108 cells/spores of phosphate-mobilizing microorganisms. Using biologically activated mordenite-containing tuff in the dose 5 t per hectare as measure for 3 years provides increasing amount of phosphate-mobilizing soil microorganisms by 14-16 times and phosphatase activity by 3-4 times as compared with addition the same dose of conventional mordenite-containing tuff. Also, the content of mobile phosphorus in soil increases from 13-33 to 31-40 mg/kg, nitrate nitrogen from 18-31 to 21-48 mg/kg, and exchangeable potassium from 69-81 to 89-98 mg/kg.
Method for preparing organic fertilizer by utilization of cellulose-containing industrial waste / 2257366
Method involves mixing cellulose-containing industrial waste with soil, its addition to prepared mixture of microorganisms, moistening the mixture and its incubation. Before addition of microorganisms to the mixture biocompost is to the mixture additionally prepared by processing avian dung, cattle manure, turf and saw-dust and soil, cellulose-containing industrial waste and biocompost are stirred in the ratio = 4:1:1, respectively. As microorganisms added to the prepared mixture method involves addition thermophilic cellulolytic microorganisms Clostridium thermocellum and Streptomyces sp. As a cellulose-containing industrial waste the method involves using the paper bulk representing waste in paper and pulp industry. Prepared mixture is wetted. Method provides acceleration of the utilization process of cellulose-containing industrial waste and to enhance quality of preparing organic fertilizer. Invention can be used in processing and utilization of cellulose-containing industrial waste resulting to preparing organic fertilizers.
Biopreparation for enhancing productivity of agricultural crops / 2264999
Invention proposes a biopreparation that comprises a mixture of live cultures Rhodopseudomonas palustris 100-I, Rhodobacter spheroids 5, Lactobacillus casei 21, Lactobacillus plantarum 51, Lactococcus lactis 47 and Saccharomyces cerevisiae 22 taken in the required ratio of components. The biopreparation enhances productivity of agricultural crops, stimulates the development of natural microbial soil and plants.
 Method of storage of bacterial fertilizers / 2265000
The invention is pertaining to the field of agriculture, namely to production and storage of bacterial fertilizers and may be used at cultivation of leguminous grasses and other agricultural plants. The method of production and storage of bacterial fertilizers includes growing of the maternal crop, making of a working crop, after that it is necessary to conduct an inoculation of the prepared peat - a massive introduction of the liquid crop into the peat. The invention provides, that the organic carrier - transitional peat - is introduce with a water solution of sodium selenate so, that the final concentration of the sodium selenate makes 1·10-4 g/kg of peat. The method allows to decrease significantly percentage of the loss of the bacterial cells on the carrier, to improve vitality and activity of the nitrogen fixers brought from a nutrient medium onto the organic carrier, and considerably to increase duration and a temperature range of storage of the bacterial preparation.
 Method for compost production / 2266883
Claimed method includes blending of waste from poultry industry or stockraising with microbial materials providing solid phase fermentation of obtained mixture. Consortium based on strains of bacterium Baccilus subtilis, fungi Trichosporon cutaneum, Trichoderma viride Sp., Fusarium sambucinum and yeast S.cerevisiae diastaticus taken in equal ratio is used as composting stimulator. Consortium is introduced into composting substrates in form of liquid suspension or dehydrated biomass in concentration of 0.005-0.05 % based on composting mixture, conditioned at temperature of 20-65°C and humidity of 35-50 % for 4-7 days depending on retreating waste composition. Dolomite chips are added into compost in amount of 5 wt.% based on composting mixture.
Biofertilizer production process / 2280629
Process comprises preparation of liquid bacterial culture, provision of biohumus by culturing of ringed earthworms on organic agricultural and industrial wastes. Thus obtained biofertilizer is sterilized in autoclave for 0.8-1.2 h at pressure 0.8-1.2 atm. Biohumus is sowed with bacterial culture, in particular pure culture Rhizobium japonicum, and then kept for 5-7 days at 20-25°C.
Method for production of biological fertilizer / 2286322
Claimed method includes composting with worms of livestock manure preliminary neutralized to pH 7-8 by using hybrid of red Californian worm with Kuban natural population of rainworm in amount of 104 per 1 m2, wherein composting with worms is carried out for 2-3 months at 16-32°C in natural conditions, and after worm isolation from biohumus microorganisms of genus Rhizobium are introduced therein in amount of 1010-1015 cells per 1 kg of biofertilizer.
Method for production of bacterial fertilizer / 2286323
Claimed method includes composting with worms of organic waste by using hybrid of red Californian worm with Kuban natural population of rainworm in amount of 104 per m2, wherein as organic waste livestock manure preliminary neutralized to pH 7-8 is used. Composting with worms is carried out for 2-3 months at 16-32°C in natural conditions, and after worm isolation from biohumus by vibroscreen with pore size of 5.5-1.0 cm biohumus is dried to humidity of 50-60 %, pre-packed in polypropylene bags, autoclaved for 45-75 min at 0.8-1.2 atm, and strain Bacillus megaterium var.phosphaticum 319 with titer of 109-1011 is introduced.
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FIELD: agriculture. SUBSTANCE: claimed method includes combination of microbiological component with natural biocompatible carrier, wherein as one part of microbiological component inoculate material of arbuscular mycorrhiza fungi (AM fungi) is used, as natural biocompatible carrier defecate form sugar beet production is used and combination thereof takes place in rizosphere of mycorrhizing plants grown of defecate. Inoculate material of AM fungi is obtained by cultivation of mycorrhized agriculture plants, for example sorgo, Sudan grass, millet, etc. on defecate. Sorgo or other mycorrhized plant seeds are seeded in containers with defecate, pure or mixed culture of AM fungi is introduced therein in form of soil-root mixture in amount of 3-5 g per one seed at occurrence level of mycorrhized roots of at least 60 %. Plants are grown for 90-120 days, then roots with defecate are dried and ground to produce pellety mixture which is blended with adhesive. Then sorgo or other mycorrhized plant seeds are treated with obtained mixture, dried and seeded in defecate and are grown for 90-120 days. Further ground plant parts and upper 20 cm of defecate together with roots are cut, and rest parts of microbiological component such as aqueous suspension of nodule-forming bacteria and/or rizobacteria. EFFECT: effective protection of culture plants, increased yield. 5 cl, 5 tbl, 2 ex
The invention relates to agriculture and recycling of food production, in particular the production of complex microbial fertilizer to improve the nutrition of agricultural crops, protection of plants against phytopathogenic microorganisms, reduction of losses of agricultural products during storage and processing of waste of sugar manufacture, and relates to a method of obtaining a complex microbial fertilizer. At the present time, there are numerous microbiological preparations for agriculture for various purposes: growth and suppress the development of pathogenic bacteria and fungi. Known "Method of seed treatment"described in application No. 2170987 UK priority from 14.02.85, MCI 4 AS 1/06 published in IMS No. 10 in 1987, this method provides for the seed treatment composition consisting of microorganisms, media such as bran, and adhesive, such as resin Gatti. The most favorable results are obtained when the treatment of wheat seeds. Microorganisms can stimulate their growth (Azospirillum), to fix nitrogen (Rhizobium)to prevent plant diseases (Pseudomonas or Bacillus), or to destroy insects (Streptomyces). Also known "Method of cabbage protection from bacterial diseases" in patent No. 1793878 priority from 10.12.90, published 07.02.93, bulletine of inventions of the Russian Federation No. 5. According to the above method for the protection of cabbage from bacterial cabbage seeds after hydrothermal treatment is treated with a suspension of a strain of bacteria "Pseudomonas sp. In-3481" at a concentration of 106-109cells/ml at the rate of 20 ml per 1 kg of seeds. The use of bacterial suspension of Pseudomonas sp. In-3481" reduces the development of vascular and mucosal bacterial diseases and increases the yield of cabbage. Also known the Method of producing the substrate for growing plants" RF patent No. 1829892 priority 20.04.90, authors Vasyuchenko I.K., Perebityy A.N., Pigulevskaya B.C., Berezko mathematical SCIENCES. and Puchko VN The invention relates to methods for producing substrates for growing seedlings and vegetable crops using biomass of bacteria of the genus Pseudomonas suppressor phytopathogens. In this way we obtain a substrate by depositing peat, lime, mineral and bacterial fertilizers with subsequent mixing of the components, as well as bacterial fertilizer use biomass of the bacterial strain Pseudomonas putida fluorescens CMPM IN-3481. Also known the Method of producing biofertilizer" RF patent No. 2130005 priority from 06.04.1996, authors Rahmanova IT, Alimova REVEALED, Ozhiganovo Hans URS, Khabibullina RE, Krylova NI, Fattakhova A.N. The method consists in the fact that the strain or community of microorganisms sterilin is cultivated in a nutrient medium to achieve a titer of bacterial mass 10 8-109cells/ml of the resulting biomass is separated from the environment, concentrate. The concentrated biomass is applied on the dried granulated chicken manure. Immobilized thus biomass is dried. This method ensures that the increased survival of microorganisms and increase the biological activity of the soil. A well-known group of inventions "Method presowing treatment of seeds of vegetable crops and the method of producing drug for presowing treatment of seeds of vegetable crops" RF patent No. 2140138 priority from, 13.11.98, authors Cebotari VK, Bykova NV, Dark O.V., Orlova N.A. and Hotyanovich AV These methods are carried out using biofungicides drug containing the bacterial strain Bacillus subtilis H-13 (deposited under registration number ARRIAM D-606 in the group of epiphytic microorganisms). The method of obtaining biofungicides preparation is to mix the culture fluid containing the strain B.s. H-13, pre-cultivated in a sterile liquid nutrient medium, emulsion PVA, water and sterile diluent chalk or dolomite in certain proportions. The invention allows to increase the effectiveness of protection of vegetable crops against plant pathogenic fungi by pre-sowing treatment of seeds. Known invention "Method of production of fertilizer p is the RF patent № 2241692 priority of 11.10.2002, authors Cebotari VK, Kazakov AE, Erofeev SV This method is combination of granulated mineral fertilizers on microbial biomass in the form of the bacterial preparation on the basis of the strain Bacillus subtilis H-13", which has antagonistic properties to pathogens. and growth promoting action. The combination is produced by coating on the surface of fertilizer granules bacterial preparation in the form of a dry powder (powder)or liquid fine fraction (spraying), or a liquid fraction comprising a waterproof compositions of mineral fertilizers. This method improves the efficiency of plant protection from infectious diseases, penetrating together with mineral fertilizers. It is also known Bacterial fertilizer "NICK" and the method of obtaining a bacterial fertilizers for application No. 99100664 priority from 21.01.99, authors Vinogradov DEATH, Vinogradov AE (prototype). Granular bacterial fertilizer contains a bacterial culture of the strain Bacillus mucilaginosus (VCMB 1451D), the sorbent and the remains of the culture fluid with metabolites, at a specific ratio of ingredients. At the same time as a sorbent of this fertilizer may contain pulverized plant mass, peat, vermiculite. In the particular case of the cultivation of spending is when used as a growing medium of an aqueous extract of poultry manure with added molasses. A method of obtaining a bacterial fertilizers on the basis of Bacillus mucilaginosus includes the cultivation of the specified strain on liquid nutrient media-hydrolysates waste industry and agriculture, after which the resulting product is mixed with sorbent and granularit. Microbial agents has been known for over a hundred years, but often their effectiveness was insufficient or unstable, because of what they failed to play a significant role in improving the productivity of agricultural production. Recently discovered a phenomenon of integration of the genetic systems of microorganisms and plants in the process of their interaction. Were able to show that both bacteria and plants have a symbiotic sets of genes that are "silent" in the absence of an appropriate partner, and in the presence of partners with specific genotypes is set to symbiosis. The process of establishing a symbiotic relationship this ecological process occurring in the soil. With the help of microorganisms, the plant provides its own demand for nutrients (nitrogen, phosphorus and others), microorganisms capable of protecting the plant against pathogenic fungi, and the most dangerous - soil infections, against which there is still no effective means. This system controls myrabo the ku najorganiziran signs or adaptation, which is not possessed no bacteria or plants to interaction. This aspect of plant-microbial interactions are now being studied. The task of the invention is to improve the protection of agricultural plants (crops) from phytopathogenic microorganisms and increase their productivity. This problem is solved due to the fact that the method of obtaining microbiological fertilizer, consisting in the combination of microbiological component of natural biocompatible carrier as one part of the microbiological component used inoculation material fungi arbuscular mycorrhizal fungi (AM fungi), as a natural biocompatible carrier use defecation, which is the filtration and washing the precipitate of sugar beet production, and their combination occurs in the rhizosphere microsulis plants when growing these plants on the defect, and then the resulting substrate add the remaining part of the microbiological component, namely pre-cultured on liquid nutrient media nodule bacteria and rhizobacteria. It is known that arbuscular mycorrhiza (AM) is a mutually beneficial plant-fungal symbiotic system (Labudova L.A., Provorov N.A., Tikhodeev O.N., Tikhonovich I.A. and other Genetics development of the Oia plants. SPb.: Science, 2000, str-384) and that AM fungi can develop only in symbiosis with the plant. Genetic factors of plants, regulatory interaction, are universal in relation to symbiotic fungi and bacteria and is present in most cultural (microsulis) plants. Symbiotic genes or part of them provide the possibility of entry into mycorrhizal symbiosis, optimizing minerals, including phosphate nutrition of plants. The property to form mycorrhiza on the roots have 80-90% of all plants. From the literature it is also known that the most effective in getting inoculating material fungi arbuscular mycorrhiza plants sorghum, sorghum-sudanly hybrid Sudan grass and millet (e.g., Labudova L.A., Provorov N.A., Tikhodeev O.N., Tikhonovich I.A. and other Genetics of plant development. SPb.: Science, 2000, str-384). Therefore, to obtain inoculating material using one of the above plants (sorghum, sorghum-sudanly hybrid Sudan grass or millet). With the goal of occurrence of symbiosis for education AM when planting seeds microsulis plants in the substrate (soil), which produce their cultivation, make a pure culture or a mixture of cultures of AM fungi in soil-root mixture under militancy plants (Karatygin IV is evolucia fungi and plants // proceedings of the Botanical Institute of the Russian Academy of Sciences, 1993, 9, p.1-118). While experimentally it was found that good results for the purpose of education AM are achieved when using as a substrate (soil) to grow microsulis plants defecation - filtration-washing sludge production of sugar from sugar beets. Defecation - defecation dirt, waste sugar beet production, containing lime, is formed in the process of purification of beet juice. Output defecation dirt 8-12% by weight of processed beets. Fresh defecate about 40% water. Dried to a friable state (moisture content of 25-30%) defecation contains: 60-75% carbonate of lime, mixed with caustic), 10-15% of organic substances, 0,2-0,7% nitrogen, 0.2 to 0.9% phosphorus (P2O5), 0.5 to 1% potassium (K2About), some amount of magnesium, sulphur and trace elements. Defecation is a good lime fertilizer, which is used for liming sod-podzolic and gray forest soils, podzolic and leached chernozems, mainly in the areas of beet growing (Large Soviet encyclopedia). That is, defecation, which is the waste of sugar manufacture, at the same time is a good soil improver improves aggregatively, structure (porosity, friability), stabilizes its acidity (PH), increases the exchange capacity of absorption of moisture by the soil moisture is the capacity). In addition, the method of obtaining microbiological fertilizer defecate allows you to utilize waste of sugar manufacture. This inoculation material fungi arbuscular mycorrhizal fungi receive, growing milisauskas agricultural crops, such as sorghum, Sudan grass, sorghum-sudanly hybrid, millet, using as ground defecation - filtration-washing the residue of sugar production from sugar beets. For this purpose, seeds of sorghum or other milisauskas plants are planted in the vessels, boxes or other containers with defecation, defecation make a pure culture or a mixture of cultures of AM fungi, for example, Glomus intraradices, i.e. the soil-root mixture under militancy plants at the level of occurrence militancy roots (usually length) not less than 60% of the calculation 3-5 g such soil-root mixture on one seed. It was established experimentally that the use of soil-root mixture at the level of occurrence militancy roots less than 60% does not obtain a sufficient number of AM in inoculation material. Also found empirically that the optimal ratio of the soil-root mixture on one seed (plant) is 3-5 g, since a smaller number reduces the effect, but more does not increase the s. Plants (sorghum or other) is grown during the vegetation period of 90-120 days. During this time, the plants form a well-developed mycorrhiza. Usually a percentage of minoritatii plants (e.g. sorghum) by the end of this period of 90-100%. Then the aboveground part of the plants are cut and removed. The remaining roots together with the substrate (defecation), dried at t° 30-50°to a moisture content of 10-12% and pulverized to obtain fractions of 0.05-0.2 mm From the obtained powder inoculating material AM fungi prepare dragonhouse mixture for further processing of its seeds microsulis plants, mixing inoculation material with adhesive in a ratio of: 80-90% - inoculation material AM fungi; the rest adhesive. It is found experimentally that the optimum result is obtained when the above ratio of ingredients. Adhesive (adhesive) is used to attach inoculating material AM fungi to the seed plants. As the adhesive can be any non-toxic to microorganisms and the treated seeds adhesive, for example, resin Gatti or 15%aqueous solution of polyvinyl acetate emulsion (PVA). In contrast to the resin Gati, PVA glue has a low value. Then the seeds of sorghum (or other milisauskas plants) has processed rajasuya the mixture at the rate of 10-15 g of a mixture of 100 g of seed, dry them at a temperature of 30-50°to a moisture content of 10-12%. Processed in this way the seeds are planted in defecation, which is the filtration and washing the precipitate production of sugar from sugar beet, in special containers, greenhouses or in designated storage defecate after filtering (e.g., filtration fields, located near sugar mills, and grown using recommended technology of cultivation of the crop. At the end of the growing season, after 90-120 days, the aboveground part of the sorghum plants (or other milisauskas plants) cut (cut) and remove. The upper 20 cm of the soil - defect together with the roots of the plants are cut and add the pre-grown in liquid nutrient media nodule bacteria in a water suspension with a titer of 107-109CFU/ml in quantity (0.5 to 5)%, and rhizobacteria in the form of an aqueous suspension with a titer of 106-108CFU/ml in quantity (0.5 to 5)% by weight of soil. Nodule bacteria are well known (for example: 1). Genetics of symbiotic nitrogen fixation with the basics of breeding. Edited Youthanasia, Nagpapatawa. St. Petersburg: Nauka, 1998; 2). N.I. Novikova Modern concepts of phylogeny and systematics of nodule bacteria. Microbiology, 1996, T, No. 4 str-450). Nodule bacteria contribute to the fixation of m is molecular nitrogen from the atmosphere and transfer it to the plant - the owner, which increases the nitrogen nutrition of plants and increased yields. In addition, part of the nitrogen remains in the roots and in the soil, improving its agricultural status. It is found experimentally that the optimum result is obtained when using a water suspension nodule bacteria with title 107-109CFU/ml in quantity (0.5 to 5)%, because their smaller concentration (titer) and the number (%) leads to a decrease of the effect, and more - not leads to its increase. Nodule bacteria previously grown 48-72 hours at a temperature of t° 28°C and pH 6.8 in the liquid Mannino-yeast medium of the following composition (g/l): 0,5 - K2NRA4; 0,2 - MgSO4×7H2About; about 0.1 - NaCl; 10,0 - mannitol, 0.5 to yeast extract; 15,0 - agar-agar to obtain a titer of 107-109CFU/ml Associative rhizobacteria also well known (for example, Kamenev SV, Muronetz E.M. Genetic control of processes of interaction of bacteria with plants in associations. Genetics, 1999, T.35, No. 11, str-1494). In the case of associative symbiosis on plant roots does not create new visible structures, but on the surface of roots in strictly defined areas are formed colonies rhizosphere bacteria that are capable of providing to the plant a number of useful features, among which, in particular: - associative AZ who fixate in the amount of up to 50 kg of nitrogen per hectare per year; - production of plant hormones that help accelerate the growth of the root system and thus ensure plant success in capturing the necessary nutrition and also regulate plant development; - optimization of absorption of phosphate remote connections. - induction of systemic reactions for defence against phytopathogens; - restriction (biocontrol) growth of pathogenic fungi on the roots of plants through such mechanisms as the selection antibiotic compounds, dissolution hyphae of pathogenic fungi, competition for space colonization on the roots, the interception of nutrients necessary for the development of phytopathogen and other It is found experimentally that the optimum result is obtained when using a water suspension of rhizobacteria with title 106-108CFU/ml in quantity (0.5 to 5)%, because their smaller concentration (titer) and the number (%) leads to a decrease of the effect, and more - not leads to its increase. Rhizobacteria pre-grown 48-72 hours at a temperature of t° 28°C and pH 6.8 in standard liquid medium TSB (tripcony soy broth), manufactured by SIGMA, prior to the receipt of title 106-108CFU/ml The obtained substrate from the defect with mikrosomalnami by plant roots, mixed with a suspension of nodule bacteria and suspension of rhizobacteria, used for PR is for drinking, preparing complex microbial fertilizer (CMU) in powder form or granular form. To obtain microbiological fertilizer (CMU) in powdered form blend of the substrate from the defect with plant roots, mixed with suspensions of nodule bacteria and rhizobacteria obtained above is dried at a temperature t° (30-50)°to a moisture content of 10-12%, grind to a fraction of 0.05-0.2, and then Packed. While drying inoculating material treated dragonhouse seed mixture, and the obtained complex microbial fertilizer carried out by any method at a temperature of not providing thermoreversible impact on the seeds of plants and arbuscular mycorrhiza on the remaining roots of the plants in the fertilizer and bacteria, i.e. not more than 55°With, as well as to the state of humidity, not below the natural seed, AM and bacteria, i.e. to a moisture content of not lower than 10%. Experiments have shown that the optimum result is obtained when the drying inoculating material, seeds, and complex microbial fertilizer at t° (30-50)°to moisture content (10-12% and its further refinement, for example, the mill Nossen 825 (VEB Maschinen Aniagenbau Nossen), to obtain fractions of 0.05-0.2 mm For the preparation of complex microbial fertilizer (CMU) in granular form a mixture of defecation with plant roots, mixed with suspensions of nodule bacteria and rhizobacteria is, obtained as indicated above, serves on the granulator (for example, brand Ohm-0.8 or OGB-1.5) and receive depending on the desired modification of granules with a size of 1-10 mm, which are then packaged. In the first the particular case of the implementation of the proposed method of obtaining complex microbial fertilizer (CMU), preparation dragonhouse mixture for seed treatment microsulis plants (sorghum or other), are used not only inoculation material AM fungi obtained at the first step of the claimed method, and pre-cultured in a liquid nutrient medium nodule bacteria. For this nodule bacteria previously grown 48-72 hours at t°=28°C and pH 6.8 in the liquid Mannino-yeast medium of the following composition (g/l): 0,5 - K2NRA4; 0,2 - MgSO4×7H2About; about 0.1 - NaCl; 10,0 - mannitol, 0.5 to yeast extract; 15,0 - agar-agar to obtain opt. title 107-109CFU/ml Thus, dragonhouse mixture for seed treatment microsulis plants (sorghum or other) on the basis of inoculating material AM fungi, obtained as described above in the first step of the claimed method, is prepared of the following composition (in % by weight of the mixture): 70-75% - inoculation material AM fungi; 10-15% aqueous suspension of pre-grown in liquid nutrient medium is e nodule bacteria with title 10 7-109CFU/ml; the rest adhesive. Then the seeds of sorghum or other milisauskas plant processes the received dragonhouse mixture, dried and grow from them defecate plants before the end of the vegetation period, as described above, after which the aboveground part of these plants are removed, and the upper 20 cm of the soil - defect together with the roots of the plants are cut and used for preparation of complex microbial fertilizer (CMU) in powder form or granular form, adding, as described above, pre-grown in liquid nutrient media nodule bacteria and rhizobacteria. While experimentally it was found that during the period of growing plants from seeds, and during the vegetation period, the concentration (titer) of nodule bacteria on the roots of sorghum or other microsulis plants decreases sharply compared with titers in dragonhouse mixture. So on the last step of the method of obtaining the CMU in defecation with plant roots add not only pre-grown in liquid media rhizobacteria, but nodule bacteria for optimum titer in the CMU. Especially effective is the use of the CMU received this (first) a special case of implementing the inventive method, as fertilizer DL the bean microsulis agricultural crops. Nodule bacteria contribute to the fixation of molecular nitrogen in symbiosis with plants, especially legumes, special symbiotic organs nodules and transfer it to the plant host, which increases the nitrogen nutrition of the plant and increases its yield. In addition, part of the nitrogen remains in the roots and in the soil, improving its agricultural status. The second particular case of the implementation of the proposed method of obtaining complex microbial fertilizer (CMU), preparation dragonhouse mixture for seed treatment microsulis plants (sorghum or other), are used not only inoculation material AM fungi obtained at the first step of the claimed method, and pre-cultured in a liquid nutrient medium of rhizobacteria. For this rhizobacteria pre-grown 48-72 hours at a temperature of t°=28°C and pH 6.8 in standard liquid medium TSB (tripcony soy broth) produced by SIGMA, to obtain opt. title 106-108CFU/ml Thus, dragonhouse mixture for seed treatment microsulis plants (sorghum or other) on the basis of inoculating material AM fungi, obtained as described above in the first step of the claimed method, is prepared of the following composition (in % by weight of the mixture): 70-75% - inoculation mA is erial AM fungi; 10-15% aqueous suspension of rhizobacteria with title 106-108CFU/ml; the rest adhesive. Then the seeds of sorghum or other milisauskas plant processes the received dragonhouse mixture, dried and grow from them defecate plants before the end of the vegetation period, as described above, after which the aboveground part of these plants are removed, and the upper 20 cm of the soil - defect together with the roots of the plants are cut and used for preparation of complex microbial fertilizer (CMU) in powder form or granular form, adding, as described above, pre-grown in liquid nutrient media nodule bacteria and rhizobacteria. While experimentally it was found that during the period of growing plants from seeds, and during the vegetation period, the concentration (titer) of rhizobacteria on the roots of sorghum or other microsulis plants decreases sharply compared with titers in dragonhouse mixture. So on the last step of the method of complex microbial fertilizer in defecation with plant roots add not only pre-grown on liquid media nodule bacteria, but rhizobacteria to ensure their optimal titer in the CMU. Colonies of rhizospheric bacteria provide the plant is a whole range of useful features, including associative nitrogen fixation, accelerate the growth of the root system, improve the absorption of phosphate compounds that protect against phytopathogens, etc. The most effective is the use of the CMU received this (second) a special case of implementing the inventive method, as fertilizer for nabibovich microsulis agricultural crops. In the third special case of the implementation of the proposed method of obtaining complex microbial fertilizer when preparing dragonhouse mixture for seed treatment microsulis plants (sorghum or other) are used not only inoculation material AM fungi obtained at the first step of the claimed method, but nodule bacteria and rhizobacteria, pre-cultured on liquid nutrient media under conditions described above. Thus, dragonhouse mixture for seed treatment microsulis plants (sorghum or other) on the basis of inoculating material AM fungi, obtained as described above in the first step of the claimed method, is prepared of the following composition (in % by weight of the mixture): 60-65% - inoculation material AM fungi; 10-12,5% aqueous suspension nodule bacteria with title 107-109CFU/ml; 10-12,5% aqueous suspension of rhizobacteria with title 106-108CFU/ml; the rest adhesive. Then the seeds SOR is about or another milisauskas plant processes the received dragonhouse mixture, dried and grow from them defecate plants before the end of the vegetation period, as described above, after which the aboveground part of these plants are removed, and the upper 20 cm of the soil - defect together with the roots of the plants are cut and used for preparation of complex microbial fertilizer (CMU) in powder form or granular form, adding, as described above, the pre-cultured on liquid nutrient media nodule bacteria and rhizobacteria. Nodule bacteria contribute to the fixation of molecular nitrogen from the atmosphere and transfer it to the plant host, which increases the nitrogen nutrition of the plant and increases its yield. In addition, part of the nitrogen remains in the roots and in the soil, improving its agricultural status. Rhizosphere bacteria provide the plant a number of useful functions, including associative nitrogen fixation, accelerate the growth of the root system, improve the absorption of phosphate compounds that protect against phytopathogens. While experimentally it was found that during the period of growing plants from seeds, and during the vegetation period, the concentration (titer) of nodule bacteria and rhizobacteria on the roots of sorghum or other microsulis plants decreases sharply compared with titers in dragonhouse mixture. Therefore h is the last step of the method of obtaining the CMU in the substrate from the defect with plant roots add pre-grown in liquid nutrient media nodule bacteria and rhizobacteria for optimum titers in the CMU. Collected in this way complex microbial fertilizer (CMU) allows its use to improve simultaneously nitrogen and phosphate nutrition of plants, to increase the degree of protection against biotic and abiotic phytopathogens, as well as to solve the problem of waste of sugar manufacture. Example 1. First get inoculations material fungi arbuscular mycorrhizal fungi, which in vessels with defecation capacity of 4 l, and a depth of 25 cm are planting seeds of sorghum (10-15 seeds per vessel); while in defecation make soil-root mixture under militancy plant occurrence militancy roots along the length of at least 60% at a rate of 5 g of this soil-root mixture on one seed. Planted sorghum is grown within 90-120 days. Then the aboveground part of the plants are cut and removed. The remaining roots together with defecation dried at t° (30-50)°C to a moisture content of 10-12% and pulverized in the mill Nossen 825 (VEB Maschinen Aniagenbau Nossen) to obtain fractions of 0.1-0.15 mm Then prepare dragonhouse mixture, mixing 85% received inoculating material AM fungi with 15% PVA glue. Then sorghum seeds treated with this cooked dragonhouse mixture based (10-15) g of a mixture of 100 g of the seeds, dry them at a temperature (30-50)°to a moisture content of 10-12%. Processed decree is mainly seeds are planted in defecation, placed in trays in the greenhouse layer of 20-25 cm, and grown from them plants, using conventional technology of cultivation of sorghum. At the end of the growing season, after 90-120 days, the aboveground part of the sorghum plants are cut and removed, and the formed substrate from the defect and sorghum roots out of the boxes and used for preparation of the CMU, adding 5% water suspension of pre-grown on liquid Mannino-yeast medium nodule bacteria with title 107-109CFU/ml and 5% water suspension pre-cultured in standard liquid medium TSB (tripcony soy broth) rhizobacteria with title 106-108CFU/ml CMU prepared in powdered form, for which it is dried at a temperature (30-50)°to moisture content (10-12)%, grind in the mill Nossen 825 to fraction (0.05 to 0.1)mm, then Packed in paper bags of 0,5 kg Obtained in this way, the CMU can be stored up to 2 years. Example 2. First get inoculations material arbuscular mycorrhiza fungi, as described in example 1. Then prepare dragonhouse mixture, mixing 65% received inoculating material AM fungi, 12% water suspension nodule bacteria (with title 107-109CFU/ml), 12% water suspension of rhizobacteria (with title 106-108CFU/ml) and 11% of PVA glue. Then the seeds colourability received this dragonhouse mixture based (10-15) g of a mixture of 100 g of seeds and dried them at t° (30-50)°C to a moisture content (10-12)%. Processed in this way the seeds are planted in defecation, which is the filtration and washing the precipitate of sugar production from sugar beet fields storing defect after filtration (filtration field), located next to the sugar factories, and grown using recommended technology of cultivation of crops sorghum. At the end of the growing season, after 90-120 days, the aboveground part of plants sorghum is harvested and removed. The upper 20 cm of the soil - press cakes with sorghum roots are cut off and used for preparation of the CMU, adding a 2% aqueous suspension of pre-grown on liquid Mannino-yeast medium nodule bacteria with title 107-109CFU/ml and 2% aqueous suspension of pre-cultured in standard liquid medium TSB (tripcony soy broth) rhizobacteria with title 106-108CFU/ml CMU prepared in granular form, for which he serves on the granulator brand OGB-1.5 and get the granules of 1.5 mm, which are then Packed in polyethylene bags of 2 kg Obtained in this way, the CMU can be stored up to 2 years. Experimental evidence for the efficiency of complex microbial fertilizer (CMU), obtained by the claimed method are presented below in tables. If the volume is to compare the effectiveness of the CMU as a reference taken microbiological fertilizer Extrasol, made on the basis of the above patent RF № 2140138.
As shown in table 1, complex microbial fertilizer (CMU), obtained according to claim 1 of the proposed method possesses a number of valuable properties, such camrost-stimulation of plants and the growth inhibition of phytopathogenic fungi. The activity of the CMU higher than Extrasolar taken for comparison as a reference.
As can be seen from table 2, when pre-treatment of seeds of pea varieties "Tatiana" and half (50% NPK) and full (100% NPK) dose of complex microbial fertilizer, obtained according to claim 2 of the proposed method, there was also a significant positive effect on pea plants. Thus, the mass of plants increased by 31.5-43,0%, and the weight of seeds per plant 21.4-of 37.8%. By all indicators the CMU is more effective than Extrasol taken as a reference.
As can be seen from table 3, when pre-treatment of seeds of spring wheat cultivar "Peasant" and half (50% NPK) and full (100% NPK) dose of complex microbial fertilizer, obtained according to claim 3 of the proposed method, a significant positive impact on important traits of plants. So, when full to the e fertilizers mass of plants increased 81.6%, energy tillering increased 1.7 times, and the number of seeds per plant by 91%. By all indicators the CMU is more effective than Extrasol taken as a reference.
As can be seen from table 4, when pre-treatment of tubers of potato varieties "Elizabeth" complex microbial fertilizer, obtained as described in step 4 of the proposed method, which also had a significant positive effect on yield and quality of potatoes. So, using the specified fertilizer CMU increased the tuber yield 35.5 t/ha, which represents an increase of 30.7%. The number of healthy tubers increased by 11%and the number of market potatoes (I+II) increased by 18.5% compared to control.
Table 5 shows that pre-treatment of seeds pumpkin varieties "Stefanova" complex microbial fertilizer, obtained according to claim 1 of the proposed method, the resulting increase in harvest pumpkin in the 3.35-fold compared with control. While CMU is more effective than Extrasol taken as a reference. 1. The method of obtaining complex microbial fertilizer, sanlucas is the action scene in the combination of microbiological component of natural biocompatible carrier, characterized in that as one part of the microbiological component used inoculation material fungi arbuscular mycorrhizal fungi (AM fungi), as a natural biocompatible carrier use defecation, which is the filtration and washing the precipitate of sugar beet production, and their combination occurs in the rhizosphere microsulis plants when growing these plants on the defect, and inoculation material AM fungi receive, growing milisauskas agricultural crops, such as sorghum, Sudan grass, sorghum-sudanly hybrid, millet, defecate, what seeds of sorghum or other milisauskas plants are planted in containers with defecation, there make clean culture or a mixture of cultures of AM fungi in soil-root mixture under militancy plants at the level of occurrence militancy roots not less than 60% of the calculation 3-5 g such soil-root mixture on one seed grown plants are planted within 90-120 days prior to the end of the growing season, then the aboveground part of the plants are cut and removed, and the remaining roots together with defecation is dried at a temperature of 30-50°to a moisture content of 10-12% and pulverized to obtain fractions of 0.05-0.2 mm, and then prepare dragonhouse mixture, mixing the obtained inoculation material AM fungi with adhesive in which the ratio (% by weight): 80-90 - inoculation material AM fungi else - adhesive, then the seeds of sorghum or other milisauskas plant processes the received dragonhouse the mixture at the rate of 10-15 g of a mixture of 100 g of the seeds, dry them at a temperature of 30-50°to a moisture content of 10-12%, planted in defecation and grow plants within 90-120 days prior to the end of the growing season, then the aboveground part of the plants are cut and removed, and the upper 20 cm of the defect together with plant roots are cut off, and then the resulting substrate add the remaining part of the microbiological component of the pre - cultured on liquid nutrient media nodule bacteria in a water suspension with a titer of 107-109CFU/ml in the amount of 0.5-5%, and rhizobacteria in the form of an aqueous suspension with a titer of 106-108CFU/ml in the amount of 0.5-5% by weight of the substrate. 2. The method of obtaining complex microbial fertilizer according to claim 1, characterized in that dragonhouse mixture for seed treatment microsulis plant-based inoculating material AM fungi is prepared of the following composition (% by weight of the mixture): 70-75 - inoculation material AM fungi 10-15 - water suspension nodule bacteria with title 107-109CFU/ml, the rest adhesive. 3. The method of obtaining the comp is exnovo microbiological fertilizer according to claim 1, characterized in that dragonhouse mixture for seed treatment microsulis plant-based inoculating material AM fungi is prepared of the following composition (% by weight of the mixture): 70-75 - inoculation material AM fungi 10-15 - water suspension of rhizobacteria with title 106-108CFU/ml, the rest adhesive. 4. The method of obtaining complex microbial fertilizer according to claim 1, characterized in that dragonhouse mixture for seed treatment microsulis plant-based inoculating material AM fungi is prepared of the following composition (% by weight of the mixture): 60-65 - inoculation material AM fungi 10-12,5 - water suspension nodule bacteria with title 107-109CFU/ml, 10-12,5 - water suspension of rhizobacteria with title 106-108CFU/ml, the rest adhesive. 5. The method of obtaining complex microbial fertilizer according to claim 1, characterized in that as the adhesive for the preparation of dragonhouse mixture using PVA glue.
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