Method for production of peat-grains compost |
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IPC classes for russian patent Method for production of peat-grains compost (RU 2296732):
Consortium of bifidobacterium strains to prepare lactic fermented and non-fermented food products, biologically active additives, leavens, bacterial preparations, hygienic agents, and cosmetics / 2296158
Invention relates to food processing, cosmetic, biotechnological, and medicinal industries. Consortium is prepared by way of combining eight bifidobacterium strains: B. bifidum 79-37, LVA-3, B. Longum V79M, Ya-3, Ya-4, B. breve 79-88, B. Unfantis 79-73, B. adolescentis GO-13. Proposed eight-strain consortium is grown on nutrient media to accumulate production mass within short culturing terms, ferments milk to form clot having good flavor and organoleptic properties, and possesses specific activity. Eight-strain consortium showing high specific activity, is capable of rapidly ecizing in human bowels and promotes normalization of gastric microflora within short periods of time. Consortium may also be a source of therapeutical and prophylactic food products having good organoleptic properties.
Consortium of bifidobacterium strains to prepare lactic fermented and non-fermented food products, leavens, biologically active additives, bacterial preparations, hygienic agents, and cosmetics / 2296157
Invention relates to food processing, cosmetic, biotechnological, and medicinal industries. Consortium is prepared by way of combining four bifidobacterium strains: Bifidobacterium bifidum 79-37, LBA-3, B. longum B379M, and Ya-4. Consortium is actively grown on nutrient media to accumulate production mass within short culturing terms, ferments milk to form clot having good flavor and organoleptic properties, and possesses specific activity. Four-strain consortium, showing high specific activity, is capable of rapidly ecizing in human bowels and promotes normalization of gastric microflora within short periods of time. Consortium may also be a source of therapeutical and prophylactic food products having good organoleptic properties.
 Strain bifidobacterium lactic 672 used to prepare lactic therapeutic and prophylactic products, fermented and non-fermented food products, biologically active additives, probiotic, and cosmetics / 2296156
Invention is intended for use in, food processing, cosmetics, biotechnological, medicinal industries, and veterinary in order to strengthen health, in particular when preparing lactic fermented and non-fermented food products, cosmetics, biologically active additives, and probiotic. Strain Bifidobacterium lactis 672 BKPM As-1677 is isolated from contents of healthy baby bowels. Invention enhances biological activity of the strain and its resistance to environmental conditions, accelerates growth on artificial nutrient media and acid-forming and antagonistic activity with regard to pathogenic and conditionally pathogenic microflora. Preparation of strains possessing above properties promotes extension of assortment of probiotics used to maintain health, in particular to normalize microflora gastroenteric and urogenital tracts, human and animal cutaneous coverings and mucous membranes.
 Bacterium strain borrelia garinii bgvir-1 for preparation of drugs for diagnosis and prophylaxis of ixodic mite-born borreliosis and for evaluation of protective and specific activity of prophylaxis and therapeutic agents / 2295564
Bacterium strain Borrelia garinii BgVir-1 synthesizes all basic immunogenic proteins of borrelia from group B. burgdorferi s.L and is useful in production of diagnostic preparations and vaccine.
 Bacterium strain lactococcus lactis subcpecies lactis b-8558 useful in dairy product preparation and method for production of starter culture of strain lactococcus lactis subcpecies lactis b-8558 / 2295563
Invention relates to new natural strain of lactic acid bacteria capable of exopolysaccharide synthesis in amount sufficient to improve rheologic product characteristics without introducing of food supplement and to increase functional properties of products producing with said strain. Starter culture of claimed strain is produced by fermentation under condition of semi-continuous deep culturing on broth containing milky whey, concentrated hydrolyzed milk, KH2PO4, Na2HPO4, MgSO4, sodium citrate, and distilled water. Fermentation is carried out at 22-32°C, constant pH 6.0-7.0 up to accumulation of living cell amount of 8.4-9.5 lg CFU/cm3. Biomass is separated, resuspended in protective medium, wherein as cryoprotector defatted milk with increased mass part of dry matter (up to 15 %) or defatted milk blended with gelatin in equal ratio is used; frozen and lyophilized. After drying biomass in bottles is sealed or pre-packed in sealed packing materials.
Bacterium strain bacillus spp. kr-083 as plant protection agent against phytopathogenic microorganism and for growth stimulation thereof / 2295562
Disclosed is new strain of rhizosphere bacteria bacillus spp. KR-083 as plant protection agent against phytopathogenic microorganism and for growth stimulation thereof. Claimed strain is isolated from South Corey rice roots.
 Method for production of medium for mycobacterium tuberculosis (mbt) culturing / 2295561
Claimed method includes blending of salt base comprising of mineral salts with glycerol, dissolution of salt base and glycerol mixture, providing of malachite green solution, egg mass providing and blending of prepared solutions with egg mass; wherein salt base and glycerol mixture is dissolved in 0.1 % sodium humate solution, and components are used in the next amount: magnesium sulfate (MgSO4 x 7H2O) 0.5 g; sodium citrate (C6H5O7Na3 x 5.5H2O) 0.1 g; ferrous-aluminum alum (Fe(NH4)(SO4)2 x 12H2O) 0.05 g; monosubstituted potassium sulfate (KH2PO4) 20.0; monosubstituted ammonium citrate (C6H11O7N) 5.0; monosubstituted sodium glutaminate (C3H8NaO4 x H2O) 10.0; glycerol (C3H8O3) 20.0 ml; 0.1 % sodium humate (C39H68O26N3)n solution up to 1000.0 ml. Method of present invention make it possible to produce the first generation of MBT cultures by 3 days earlier and to increase biomass by 2.1 times in relates to control.
Method of preparing bacterial agent rhoder for cleaning soils, lands, oil slimes, fresh and mineralized waters to remove petroleum and petroleum product pollution / 2295403
Invention relates to preparing oil destruction agents suitable for cleaning oil-polluted soils and water. In particular, bacterial agent Rhoder is based on strains Rhodococcus rubber BKM As-1513D and Rhodococcus erythropolis BKM Ac-1514 D and prepared in several steps. Preparation procedure is distinguished by that, in the first step, most active clones of strain R forms belonging to oil destruction agent are selected and, in the last step, common deep culturing of two strains is performed, the two strains having been preliminarily grown in inoculators on specially selected nutrient media under physiologically optimal culturing conditions in large scale. In the maximum biomass production step, biomass is grown on nutrient medium containing glucose as only carbon source and autolyzed yeast or fish hydrolyzate as source of nitrogen and other organics. Preparation according to invention enables preparing liquid or dry agent with number of active hydrocarbon-oxidizing cells 1011-1012 cell/mL and 1010-1011 cell/mL, respectively, which eliminates need in further performing preliminary activation of dry agent.
Escherichia coli strain n 280 capable of thermolabile lt-entherotoxin producing / 2293765
Disclosed is strain N 280 having high ability to produce thermolabile LT-entherotoxin and being useful in production of Escherichia coli thermolabile entherotoxin (LT-entherotoxin) and anatoxine in vaccine manufacturing.
Bacterium pseudomonas alcaligence strain useful in purification of soils, ground water and surface water from trinitrotoluene / 2292392
Invention relates to production of biopreparation for purification of soils, ground water and surface water from trinitrotoluene. Bacterium Pseudomonas alcaligenes BS300 strain was isolated from soil. Said strain utilizes trinitrotoluene, is stable to heavy metal ions and is useful in purification of soil and water from combined contamination by trinitrotoluene and heavy metal ions. Pseudomonas alcaligenes BS300 strain produces biological surfactant accelerating trinitrotoluene degradation in water medium and soil.
 Improved method for producing lycopene via fermentation of selected strains blakeslea trispora and using thus obtained lycopene / 2296161
Invention provides a group of inventions: lycopene production method, crystalline lycopene, employment of crystalline lycopene and compositions based thereon in food processing, pharmaceutical, and cosmetic industries as well as dietetic additive. Method is based on using (+) and (-) strains of fungus Blakeslea trispora, wherein (+) strain is CPAI(+) and (-) strain is selected from group consisting of L25(-), CMAI(-), CMA3(-), CMA4(-), CMBI(-), CMB2(-), LMAI(-), or combinations thereof. Method also envisages extraction of lycopene contained in purified biomass with organic solvent and simplifies isolation process and product purity increase. Thus obtained lycopene is base of composition consisting of oil suspension of crystalline lycopene and composition of lycopene dispersed in cold water.
 Method for preparing citric acid and acid-stable amylolytic enzymes complex / 2294371
Invention relates to a method for preparing citric acid and a complex of acid-stable amylolytic enzymes possessing activity of α-amylase and glucoamylase. Method involves separation of mycelium of fungus Aspergillus niger as a producer of acid from a fermented solution with the protein concentration 1.5-7.0 g/dm3 at temperature ≤32°C. Prepared fermented solution is purified by successive filtration processes through membranes with pores diameter values 0.65, 0.45 and 0.15 mcm and separated by ultrafiltration through a membrane retaining substances with molecular mass in limits 1000-50000 Da and providing preparing citric acid solution and acid-stable amylolytic enzymes solution. Citric acid solution with the protein content 0.05-0.15 g/dm3 is cleared preliminary followed by its concentrating, crystallizing and citric acid crystals are separated from mother liquor. Mother liquor with optical density value 0.1-0.4 at λmax = 400 nm is recovered to the concentrating step and solution with optical density 0.41-0.8 at λmax = 400 nm is cleared preliminary and recovered to the concentrating step. Solution of above mentioned enzymes is dried by sublimation or spraying up to preparing a dry complex with enzymatic activity of α-amylase and glucoamylase 700-900 and 10000-15000 U/g, respectively, or solution is precipitated followed by drying up to preparing the dry complex with enzymatic activity of α-amylase and glucoamylase 1000-1300 and 20000-26000 U/g, respectively. Method provides simultaneous preparing crystalline citric acid and a dry complex of acid-stable amylolytic enzymes with the high quality and high yield.
![Method for production of 4-[4-[4-(hydroxybiphenylmethyl)-1-piperidinyl]-1-hydroxybutyl]- α,α-dimethylphenylacetic acid](http://img.russianpatents.com/img_data/122/1222359-s.gif) Method for production of 4-[4-[4-(hydroxybiphenylmethyl)-1-piperidinyl]-1-hydroxybutyl]- α,α-dimethylphenylacetic acid / 2290443
4-[4-[4-(hydroxybiphenylmethyl)-1-piperidinyl]-1-hydroxybutyl]-α,α-dimethylphenylacetic acid is produced by incubation of mixture, containing terphenadine and microorganism capable to produce said acid followed by isolation of target product.
Strain of mycelial fungus trichoderma longibrachiatum as producer of carbohydrases complex comprising cellulases, β-glucanases, xylanases, mannanases and pectinases / 2287571
Invention relates to the strain Trichoderma longibrachiatum VKM F-3865D (the All-Russian collection of microorganisms in IBFM named for G. K. Skryabin, RAS) that possesses ability to produce complex of carbohydrases comprising cellulases, beta-glucanases, xylanases, mannanases and pectinases with high level of their activity. Invention provides the possibility for preparing the full complex of enzymes used for hydrolysis of vegetable raw polysaccharides and, if necessary, separate individual enzymes (components) of this complex. Invention doesn't require using complex and expensive nutrient media for achievement of high productivity of the strain. Invention can be used in different fields of alcoholic and food processing industry and in agriculture.
Strain of mycelial fungus penicillium funiculosum as producer of carbohydrases complex consisting of celluloses, β-glucanases, xylanases, pectinases and mannanases / 2287570
Invention relates to the strain of mycelial fungus Penicillium funiculosum VKM F-3668D prepared by multistep classic mutagenesis and selection methods from the parent culture of Penicillium funiculosum P1 (VKM F-3661D) and by using ultraviolet irradiation. The strain shows capacity for producing the complex of highly active carbohydrates involving celluloses, β-glucanases, xylanases, pectinases and mannanases that provides possibility for preparing the much more active and full enzyme complex and if necessary the separate individual enzymes (components) of this complex. For providing the high productivity of the strain nutrient media used conventionally by industrial technology for preparing similar enzyme preparations can be used instead using the complex and expensive nutrient media. Invention provides enhancing effectiveness and expanding assortment of enzyme preparations in different fields of biotechnology being as fodder supplements especially.
 Method for introducing of trichoderma lignorum antagonist fungus into plant phyllosphere / 2285406
Claimed method includes spraying of cereal spikes in flower phase with aqueous concentrated spore/mycelium suspension having titer of at least 100x106 cell/ml at standard processing fluid growth of 30 ml/m2.
Preparation for cleaning soil and water surfaces polluted by petroleum and petroleum derivatives / 2280013
Invention relates to means for controlling pollution of environmental objects with petroleum and petroleum derivatives using fungal microorganism and can be used, for instance, when eliminating consequences of oil spills. Preparation according to invention comprises peat filler (94.9-95.3%), oil destroying microorganisms (0.07-0.08%), water, and, additionally, sucrose (0.044-0.047), said oil destroying microorganisms being fungus strain Aspergillus niger S-1 and said peat filler being upper sphagnous moss or upper slightly decomposed sphagnous moss.
Method for microorganism isolation and culturing / 2279469
Substrate for isolation or culturing of microorganisms represents autoclaved suspension of mammalian feces with suspended particle size not more 2 mm, contains 10-7-10 g of feces per 1 l of suspension and has pH 6.0-7.6. Substrate optionally contains target additive to produce liquid or semi-liquid, or dense nutrient medium in amount of 5-300 g/l.
 Method for diagnosis of dermatomycosis and nutrient medium for dermatophytes / 2275631
Diagnosis of dermatomycosis is carried out by seeding of dermatophytes from clinical material in dense selective Sabouraud's medium with addition of antibiotic levomycetine and keratin hydrolyzate, obtained from chicken feather. Claimed nutrient medium contains (g/l): glucose 40.0; peptone 10.0; agar 18.0-20.0; keratin hydrolyzate (as calculated to dry matter) 10.0-20.0; levomycetine 50000 U; and balance: distilled water. Seeding in incubated at 22-24°C for 4-8 days followed by identification of colony species. Method is most effective in isolation from clinical materials dermatophyte fungi cultures, such as Microsporum canis, Trichophyton verrucosum, Trichophyton mentagrophytes var.gypseum, Trichophyton rubrum.
Phytase-containing aqueous liquid, method for production thereof, phytase-containing granulated material and method for production thereof, animal feed, premix or semi-finished animal feed, method for production thereof, and animal growth stimulation / 2275052
Method for phytase-containing aqueous liquid includes cultivation of genus Aspergillus microorganism transformed with expression vector, containing phytase gene of said microorganism bond to promoter and/or signal sequence of amyloglucosidase gene. Cultivation is carried out in aqueous medium containing carbon and nitrogen source under conditions, which allow recombinant phytase expression. Cultural liquid is filtered, cation exchange chromatography, anion exchange chromatography, and ultrafiltration are carried out to produce phytase-containing aqueous liquid having activity at least of 14000 U/g. Said aqueous liquid with phytase activity is useful in production of granulated material, animal feed, premix or semi-finished animal feed.
 Method for removal of manure and processing the same into humic fraction / 2294095
Method involves delivering manure into apparatus for removal of manure from animal house through main manure pipeline wherein distributing gate is positioned for directing manure through one of manure ducts toward hill. Manure is directed through manure duct bend from the bottom to the top and is poured circumferentially of hill base. Hill is grown with solid fraction from the top without disturbing of top layer. After the hill is filled with manure, it is directed by means of gate toward other manure duct to form another hill.
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FIELD: biotechnology, in particular production of environmentally friendly peat-grains compost. SUBSTANCE: claimed method includes blending of beer grains with peat in ratio of 1:1 or 1:2 followed bioactivator introducing and aerobic composting at 20-60°C and humidity of 65-70 % for 2-3 months. Compost ferment prepared by culturing of microorganism consortium, isolated from humified humous forest soil layers and containing fungi Aspergillus niger, Aspergillus oryzae, Aspergillus sydowii, Cephalosporium, Glyocladium Cda., Trichoderma sp., actinomycetes Streptomyces griseus, Streptomyces termoviolaceus, Streptomyces globisporus, Streptomyces rubber, Streptomyces viridosporus and bacteria Bacillus cereus, Bacillus mycoides, Bacillus sublilis, in amount of 15 % or more based on composing mixture mass on beer grains is used as bioactivator. EFFECT: accelerated fermentation and composting processes; compost of increased quality. 3 tbl, 2 ex
The invention relates to the biotechnology industry, in particular to the production of organic compost on the basis of waste brewing beer pellet using stimulant composting in the form of a consortium of microorganisms. A known method of producing compost by mixing waste poultry and livestock farms with moisture-absorbing materials, such as lignin in combination with straw, sawdust, peat and subsequent solid-phase fermentation at a temperature of 20-70°With the mixture for 5-7 days with the use of stimulant composting in the form of a consortium-based thermotolerant and thermophilic bacteria Bacillus subtilis, Clostridium butyricum, Micrococcus urea and fungus Sporotrichum pruinosum entered in the form of an aqueous suspension of cells or in the form of a dry powder in a concentration of 0.05-0.1% biomass by weight compostable mixture (see patent RU No. 2197453, IPC7C 07 F 11/08). The disadvantages of this method is to obtain compost with low fertilizer and soil-improving properties due to the absence of microorganisms stimulator composting of ligninases microorganisms, which leads to the production of compost that is not fully Mature, containing half-decayed remains of vegetable origin. This compost can induce the growth and reproduction phytopathogen the x fungi and bacteria, and the products of its half-life in the form of organic acids can have a negative impact on the growth and development of plants. The closest way to claimed is a method of composting, comprising mixing organic waste (litter) with shredded moisture-absorbing organic material (peat, sawdust) with the introduction of bioactivator in the form of a microbial composition comprising calculatorcredit, nitrogen-fixing and stimulating the growth of plants, microorganisms and aerobic composting mixture (see patent RU No. 2230721, IPC7C 05 F 3/00, 11/08). The disadvantage of this method is the selection of microorganisms without taking into account the temperature gradient activity of microorganisms, as used in the method thermotolerant micronized - Monoascus rubber is active only at a certain temperature, which slows down the fermentation process. The invention is industrially obtaining compost high agrochemical values based on the tonnage of waste brewing beer grain. The technical result of the claimed technical solution is to increase the intensity of the processes of fermentation and composting and improving the quality of compost. The technical result is achieved in that in the method of preparation torpedoing compost, including smeshivanie waste humic-containing component with the introduction of bioactivator and subsequent aerobic composting mixture, as waste use of nitrogenous waste in the form of brewer's grains, as humic-containing component - peat, taken in the ratio of 1:1 or 1:2, as bioactivator use compost starter, prepared according to patent 2213080 taken in not less than 15% by weight of compostable mixture, and the mixture of compost at a temperature of 20-60°C and humidity of 65-70% in 2-3 months. Preparation of compost by adding to the compost mixture bioactivator containing a consortium of micro-organisms from fungi, actinomycetes and bacteria isolated from well-humified humus layer of forest soils in accordance with the pattern of successional change the dominant microbial complexes-destructors in accordance with the sequence of changes of temperature and material composition of composted components and cultivated at the beer mash and use as feedstock compost, this beer pellet - waste brewery, which serves as a complete source of nutrition for growth and reproduction of many organotrophic organisms and, in the first place, mushrooms, participating in the initial stage of decomposition of organic residues and the preparation conditions for the growth of other groups of microorganisms responsible for the subsequent processes of transformation of substances. This PR which leads to the activation of a complex of beneficial microorganisms, intensive oxidation of organic compounds compostable mixture and the accumulation of humus substances in it, i.e. accelerates the process of fermentation and composting to produce high-quality compost with high fertilizer properties due to the presence of mineral and humic substances and soil-improving properties due to the presence of peat humic substances that stimulate the development of all soil microorganisms - fungi, bacteria and actinomycetes. Torpedoing compost is prepared as follows. Peat as a humic-containing component selected from the upper layer of the fens, are mixed in a ratio of 1:1 or 1:2 with nitrogen-containing waste - beer mash (waste brewing) and make a bioactivator in the form of compost starter culture prepared according to patent No. 2213080 way of cultivation on the beer mash consortium of micro-organisms of the fungi Aspergillus niger, Aspergillus oryzae, Aspergillus sydowii, Cephalosporium, Glyocladium Cda., Trichoderma sp., actinomycetes Streptomyces griseus, Streptomyces ter-moviolaceus, Streptomyces globisporus, Streptomyces ruber, Streptomyces viridosporus and bacteria Bacillus cereus, Bacillus mycoides, Bacillus subtilis. Compost starter contribute at least 15% by weight of compostable mixture with moisture compost mixture and of the leaven of 65-70%. Composting is conducted in open areas, maintaining the humidity of 65-70%. After 2-3 days the od action of a compost starter begins active exothermic reaction (temperature of the mixture increased to 50-55° C and above) as a result of intensive oxidation of organic compounds and accumulation of humus substances and complex of beneficial microorganisms. Composting mixture is carried out in 2-3 months, with periodic 1 time within 7-10 days, stirring it for aeration, the temperature of the mixture is gradually reduced to the level of the environment. Example 1. In laboratory conditions, peat 5 kg mixed in the tank with 5 kg of spent grains (ratio 1:1) and made a bioactivator - compost starter in the amount of 15% by weight of compostable mixture of 1.5 kg humidity of 65-70%. After 2-3 days there was an increase in the temperature of the mixture up to 40-50°C and above. The composting mixture was carried out for 2 months under stirring 2-3 times a week for aeration of the substrate. At the end of the composting conducted chemical analyses showed (see table 1)that in the process of fermentation of the compost mixture has undergone significant changes: decreased carbon content with increasing amount of nitrogen, which is reflected in a decrease in the ratio C/N. the pH Value increased to the level of neutral environment, which is explained by the action of ammonium nitrogen, cumulative process of decomposition of protein substances in the beer mash and characterized by an alkaline reaction. Significantly increased cation-exchange capacity, which is one of the and the of Doctorow quality and maturity of the compost mass.
In the composting process was observed active humification, and a higher content Gumi the OIC acid compared to fulvic acids indicates sufficient maturity of compost (see table 2).
Example 2. Peat 5 kg mixed in a container with 10 kg of spent grains (ratio 1:2) and made a compost starter in the amount of 15% by weight of compostable mixture of 2.25 kg at a relative humidity of 65-70%. The composting mixture was carried out similarly to example 1 within 2 months of the EB with periodic stirring. It is noted in comparison with the raw material (see table 1) increasing the amount of nitrogen in a form more accessible to plants, increasing the pH to the level of neutral environment, the growth of the cation-exchange capacity, which is one indicator of the quality and maturity of the compost mass. Comparison of agrochemical characteristics stated torpedoing compost with various kinds of known composts (see table 3) showed that the proposed peat-drabiny compost than the known content of nitrogen, phosphorus and potassium and slightly inferior only to vermicompost on the content of potassium and vermicompost on the amount of nitrogen.
Way that provides expedited delivery of clean compost high agrochemical values based on the deviation of brewing, will find wide industrial application. The method of preparation torpedoing compost, comprising mixing the waste with humic-containing component with the introduction of bioactivator and subsequent aerobic composting mixture, characterized in that the waste used as nitrogenous waste in the form of brewer's grains, as humic-containing component - peat, taken in the ratio of 1:1 or 1:2, as bioactivator use compost starter, prepared by the method of cultivation at the beer mash consortium of microorganisms isolated from well-humified humus layer of forest soils, consisting of Aspergillus niger, Aspergillus oryzae, Aspergillus sydowii, Cephalosporium, Glyocladium Cda., Trichoderma sp., actinomycetes Streptomyces griseus, Streptomyces termoviolaceus, Streptomyces globisporus, Streptomyces ruber, Streptomyces viridosporus and bacteria Bacillus cereus. Bacillus mycoides. Bacillus subtilis, taken in an amount not less than 15% by weight of compostable mixture, and the mixture of compost at a temperature of 20-60°C and humidity of 65-70% in 2-3 months.
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