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Method for production of fermented agriculture composition

IPC classes for russian patent Method for production of fermented agriculture composition (RU 2296112):
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The invention is pertaining to microballs containing the derivative of pleuromutiline and the method of their production and also to production of the fodder granules containing the microballs and to the method of their production. The microballs have the average size from 1 micron up to 5000 microns and represent the polymeric matrix particles, where the polymeric compound is selected from the shellac, the polymeric compound on the basis of cellulose, the acrylic acid or the methacrylic acid, the maleic anhydride, polyvinyl pyrrolidone or polyvinyl alcohol, and which is highly dispersed in the solid or liquid form with the derivative of pleuromutiline of formula 1 or its physiologically tolerable acid-additive salts and quarternary ammonium salts: , where R1 means vinyl, between 1 and 2 carbonic atoms there is a single bond, and Ra and Rb mean each separately from each other hydrogenen or halogen, and Т represents -CH2-S-(CH2)K-N(R5)(R6), where "k" means the integer from 2 up to 5; R5 andR6 mean each separately from each other C1-C6-alkyl or Т represents -CH2-S-C(CH3)2-CH2-NH-C(O)-CH(NH2)-CH(CH3)2. The method of preparation of the microballs includes production the polymer solution selected from the shellac, the polymetric compound on the basis of cellulose, the acrylic acid or methacrylic acid, the maleic anhydride, polyvinyl pyrrolidone or polyvinyl alcohol, by dissolution of the shellac, the polymetric compound in the organic solvent, having the low affinity to the paraffinic oil or the organosilicic oil and with the dielectric constant from 10 up to 40, with addition of the water. Then the produced solution is introduced at intermixing with the derivative of the pleuromutiline and receive the first organic phase, which is not admixed with the paraffinic oil or the organosilicic oil. The first organic phase introduce at the intense intermixing into the second phase consisting of the paraffinic oil or the organosilicic oil, and prolong intermixing of the produced mixture until the microballs are formed at evaporation or the solvent removal. The microballs are separated, flushed and dried. The fodder granules for the livestock includes the effective number of the indicated above microballs, the milled dry fodder on the vegetable or animal base with or without the additives, such as proteins, vitamins and minerals. The method of preparation of the fodder granules includes the careful intermixing of the microballs with the organic, ground and homogenized components of the fodder. Then they add the water or the steam in the mixture in the amount from 5 up to 10 % of the total mass, press the mixture in the fodder rods at the heightened temperatures from 60 up to 80°С, and divide the rods into the fodder granules. The invention ensures stabilization of the pleuromutiline, which stays undamaged at the granules preparation and is kept safe for the rather long duration of storage.

FIELD: agriculture, biotechnology, production of feeds and fertilizers.

SUBSTANCE: claimed method includes microbial decomposition of plant waste in oxygen-enriched air forced circulation, wherein oxygen is obtained by Chlorella Vulgaris culturing. Carbon dioxide released in process of plant waste decomposition is collected and used in algae culturing. Method also includes manure deodorizing with calcium oxide or calcium hydroxide. Deodorized manure is mixed with mass obtained in process of plant waste decomposition. Mixture is fermented for 4-5 days and mixed with solid fraction of Chlorella Vulgaris. Said solid fraction of Chlorella Vulgaris in obtained by double freezing and defrosting of algae biomass and cell juice separation. Process is carried out in closed cycle. Obtained composition is useful both as fertilizer and feed supplement.

EFFECT: environmentally friendly method for reprocessing of plant waste and mature.

4 cl

 

The invention relates to agriculture, namely, biotechnological methods of recycling of crop residues and manure, and can be used for fodder and fertilizer.

The known method of aerobic decomposition of crop residues by soil microorganisms, leading to producing organic fertilizers [1].

A method of refining the straw into the soil to replenish the mineral and organic substances, which consists in the fact that the straw of wheat, rye, barley, oats, rice autumn are buried in the soil to a depth of 10-30 cm in a dose 4-16 t/ha [2].

The disadvantage of this method is limited to their use as fertilizers.

Known method of biofermentation a mixture of peat and manure with periodic blowing air [3].

The technical result - the development of a method of producing a feed additive and fertilizer.

The technical result is achieved by the fact that in the known method, including fermentation of the mixture of manure and organic mass in the periodic blowing a mixture of air, according to the invention as the organic mass use of plant residues, which are pre-add water in a volume ratio of 1.5-2.0:1 and subjected to microbiological decomposition of soil microorganisms in soil extracts, which UPE is Yat in number at the rate of 2 ml per 1 litre of water, the decomposition is carried out in conditions of forced aeration with air enriched with oxygen, obtained by culturing unicellular Alga Chlorella Vulgaris respiratory factor 1, and carbon dioxide (CO2) decomposition of plant residues support at 0.10-0.15 g/hour, which is captured and used for the cultivation of algae.

In addition, to achieve a technical result, the manure before fermentation deodorize the calcium oxide or hydroxide of calcium, which contribute in the amount of 0.7-0.9% of the dry matter content of the solid fraction of manure and fermentation of the mixture containing 60% of the organic mass and 40% of manure, carried out within 4-5 days, and the fermentation product is mixed with a solid fraction of Chlorella Vulgaris, received two by freezing and thawing biomass of algae and Department of cell SAP.

Microbial decomposition of plant residues is carried out in a period of not less than 3 days.

Freezing biomass algae carried out first at a temperature of minus 13 to minus 15 degrees for 40-60 minutes, again at a temperature of minus 4°C for 5 hours.

The fermentation mixture is mixed with a solid fraction of Chlorella Vulgaris in a mass ratio of 3.5 to 4.5:1.

The method is as follows.

Straw grass is in, husked corn cobs and other plant residues crushed to particle size of 6-8 cm, pour water at the rate of 1:1.5 to 2.0, (volumetric ratio), and then make the soil extract in the amount of 2 ml per 1 litre of water. Through the hole in the bottom of the vessel carry out continuous aeration with air enriched with oxygen. Air consumption is 0.5-1.0 l/min

Decomposition of plant residues is carried out in the range of physiological temperatures, does not disrupt the normal vital activity of microorganisms for at least 3 days.

In plant debris under the action of soil biocomplex happen aerobic processes associated with active development of saprophytic forms of bacteria and yeast, decomposing organic substances to form compounds, further oxidation which leads to the destruction ligninsulfonate complex.

In the process of decomposition of plant residues are monitoring the amount of carbon dioxide that is captured and used for culturing unicellular Alga Chlorella Vulgaris.

To produce 1 kg of biomass of Chlorella Vulgaris requires 2 kg of CO2respiratory factor 1. This means that the ratio of the amount of absorbed carbon dioxide to the amount of oxygen equal to one. This ratio is called digitalsinmoviescom. The process when the DC is equal to 1, goes before the formation of the final products of cleavage of the respiratory substrate. If the process is not going to end, and there is accumulation of intermediate compounds of character, then DK is reduced. This decrease is stronger than most of the respiratory substrate remains in the oxidized state and, therefore, the less stands out About2. As highlighted About2the result of the cultivation of algae captured and sent to the enrichment of the air for forced aeration during decomposition of plant residues, to obtain the best performance in terms of biomass, algae and maximum efficiency of decomposition of plant residues carbon dioxide production support at 0.10-0.15 g/hour.

As the algae use, for example, a strain With a 111-planktonic form of Chlorella Vulgaris. The optimal cultivation temperature 28-30°C, pH 6-8. Average growth of algae is 50-60 mg/L.

When the carbon dioxide of less than 0.10 g/h performance is decreased biomass of Chlorella Vulgaris.

When carbon dioxide more than 0.15 g/hour in the process, the accumulation of unusable carbon dioxide.

The solid fraction of manure from ruminants after mechanical fractionation loaded into the mixer. Humidity such manure with the hat 75%, this corresponds to a content of dry residue in the amount of 250 kg per 1 t of the initial mass. In mixer make a powdered oxide of calcium in the form of burnt lime or calcium hydroxide in the form of slaked lime in the amount of 1.75-2.25 kg per ton of the initial mass of manure that is 0.7-0.9% of the solids content of, stirring occasionally.

The essence of deodorizing manure is as follows.

Unpleasant smell of manure is due to the formation of mercaptans and hydrogen sulfide in the decomposition of the intestinal microflora of sulfur-containing amino acids: cysteine and methionine.

Calcium (oxide or hydroxide) in collaboration with the anions of the carbonic acid content in the manure is 3-5 g/kg, to form calcium bicarbonate. The latter links the mercaptans and hydrogen sulfide in connection, no smell.

Deodorizing manure before fermentation enriches derived feed additive and fertilizer essential trace elements - calcium and eliminates unpleasant smell during the whole period of fermentation mixture.

Then organic mass from plant residues in the amount of 60% is mixed with 40% deodorized manure and purge the mixture with air. The temperature 58-60°C. the Presence of air and temperature provides the active development of thermophilic microorganisms. The germ is the first mass rapidly increases in volume due to the assimilation of carbohydrates, compounds of nitrogen and phosphorus. The process lasts for 4-5 days. In the fermentation process is the destruction of pathogenic organisms.

The product resulting from the fermentation mixture contains crude protein 17-21% and non-pathogenic thermophilic microorganisms more than 30 thousand, which can serve as a source of complete bacterial protein for ruminants. The large number of thermophilic aerobic microorganisms provides favorable conditions for scar digestion.

The increased activity of the rumen microflora contributes to the formation of volatile fatty acids, which are precursors of milk fat. Moreover, the introduction to the basic ration fermentation of manure and organic mass from plant residues in the number of 150-180 g per 100 kg body weight increases the amount of volatile fatty acids on 29-31%. On the one hand, does not go beyond the limits of physiological norm. On the other hand, allows you to fully exploit the genetic potential of animals in terms of gynomonoecy.

Then the biomass of the algae Chlorella Vulgaris, resulting from cultivation, is separated from the culture fluid and subjected to two freeze, first at a temperature of minus 13 to minus 15°C for 40-60 min, repeated at a temperature of minus 4°C for 5 hours.

P the following defrost easily separated cell SAP, which connect with the culture fluid.

Derived biologically active complex (liquid) can be used in feeding (feeding) of farm animals and poultry. And solid fraction - sheath cell walls of algae contribute to the fermentation of organic mass and manure mix. The ratio of the components is 1:3,5-4,5, respectively.

In the feeding of farm animals feed additive is added to the basic diet in the amount of 140-160 g per 100 kg of live weight.

Fertilizer efficiency as productive feeding was studied in growing hybrid maize ROSS 331 MB for grain. Care of plants was carried out according to the phases of development.

At the end of the first stage of organogenesis stage 4-7 leaf begins rapid growth of the corn. During this period, conducted the first feeding is based 60-80 kg/ha, which was leafy depth 10 cm

The second productive feeding was performed before throwing panicles based 50-60 kg/ha

The dressing was made in the middle of the rows, Sapareva to a depth of 12 see This contributed to the development of the maximum number of embryonic ears.

Application of fertilizers allowed the maximum use of the biological potential of hybrid and get the grain yield by irrigation of 9.8 t/ha

First to mponent feed additives and fertilizers - the product of fermentation deodorized manure and organic mass from plant residues is quite self-sufficient and effective as from the point of view of the feed additive, and from the point of view of fertilizers.

The second component is a solid fraction algae Chlorella Vulgaris is represented 77% chitin. This polysaccharide (biopolymer), which is contained in the body of animals and plants and has a number of important properties. For example, he actively binds organic and inorganic molecules of toxic substances in the organism of animals and in the soil. In addition, the pathogens of viral, fungal and bacterial diseases cause coagulation - coalescence of particles in colloidal solutions with the formation of large agglomerates at loss coagulating substances in the sediment. Since the most frequent coagulation occurs in solutions containing proteins, it leads to loss of protein (undigested protein). Chitin has anticoagulant and antibacterial properties and therefore increases the body's resistance to viral, fungal and bacterial infections.

The cleavage products of chitin are natural and harmless, do not cause side effects.

The connection component provides a more pronounced effect and versatile action.

Microbial decomposition of plant the mistakes and cultivation of Chlorella Vulgaris, held in closed loop (highlighted in oxygen from the cultivation of algae capture and use for decomposition of plant residues, and the selected carbon dioxide capture and use for cultivation of Chlorella Vulgaris) reduces air pollution.

Sources of information

1. Galbe, Gstone. Straw as fertilizer. M.: Kolos, 1972.

2. News of TAA, Issue 3, 1978, s-33.

3. RU # 2192757, AK 1/16, publ. 20.11.2002.

1. The method of obtaining feed additives and fertilizers, including fermentation of the mixture of manure and organic mass in the periodic blowing a mixture of air, characterized in that as the organic mass use of plant residues, which are pre-add water in the ratio 1:1,5-2,0 and subjected to microbiological decomposition of soil microorganisms in soil extracts, which contribute at the rate of 2 ml per 1 l of water, the decomposition is carried out in conditions of forced aeration with air enriched with oxygen, obtained by culturing unicellular Alga Chlorella Vulgaris respiratory factor 1, and carbon dioxide (CO2) decomposition of plant residues support at 0.10-0.15 g/h, which is captured and used for culturing algae, manure per the d fermentation deodorize the calcium oxide or hydroxide of calcium, contributing in the amount of 0.7-0.9% of the dry matter content of the solid fraction of manure and fermentation of the mixture containing 60% of the organic mass and 40% of manure, carried out within 4-5 days, and the fermentation product is mixed with a solid fraction of Chlorella Vulgaris, received two by freezing and thawing biomass of algae and Department of cell SAP.

2. The method according to claim 1, characterized in that the microbial decomposition of plant residues is carried out in a period of not less than 3 days.

3. The method according to claim 1, characterized in that the freezing biomass algae carried out first at a temperature of minus 13 to minus 15°C for 40-60 min, repeated at a temperature of minus 4°C for 5 hours

4. The method according to claim 1, wherein the fermentation product is mixed with a solid fraction of Chlorella Vulgaris in a mass ratio of 3.5 to 4.5:1.

 

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