Method of biologically processing bird dung |
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IPC classes for russian patent Method of biologically processing bird dung (RU 2322427):
Biopreparation for enhancing productivity of agricultural crops and improving quality of production / 2322061
Invention relates to biopreparations of effective microorganisms used in the plant growing. Proposed preparation consists of a mixture of suspension of the following strains deposited in VKPM: Agrobacterium tumefacients B-4116, Agrobacterium radiobacter B-956, Azotobacter chroococcum B-2375, Bacillus thuringiensis B-2918, Bacillus subtilis B-6554, Bacillus subtilis B-4419, Bacillus megaterium B-4440, Bacillus megaterium B-200, Bradyrhizobium japonicum B-1978, Ervinia ananas B-5292, Lactobacillus casei B-3961, Pseudomonas fluorescens B-1138, and Rhodopseudomonas palustris B-1629. Invention provides restoring soil and to improve its structure, increase germination of seeds, fortify immune system of plants, enhance resistance against diseases and pests that increases the yield significantly and high quality of the prepared foodstuffs. Proposed biopreparations can be used for autumn, spring and summer treatment of soil, roots and leaf feeding, and for presowing treatment of seeds.
 Composition for protection of vegetable cultures against fungal and bacterial diseases / 2322060
Invention proposes a composition containing chitosan, chitin, succinic and glutamic acids, heteroauxine, and strain Bacillus subtilis M-22 or strain Trichoderma lignorum T-36. The composition possesses antagonistic activity in combination with stimulating effect on growth of plants and provides the effective protection of vegetable cultures against fungal and bacterial diseases.
Microorganism strain klebsiella pneumoniae gisk №278 as producer of lysozyme inhibitor / 2321632
Invention relates to the microorganism strain Klebsiella pneumoniae GISK № 278 isolated from a patent feces suffering from intestine dysbacteriosis. The strain is used for preparing an agent for producing the lysozyme inhibitor. The level of activity of lysozyme inhibitor produced by this strain is 1.64-2.16 mcg/ml of *OD value.
 Microorganism strain halobacterium salinarum as producer of bacteriorhodopsin / 2321627
Invention relates to microorganism strain Halobacterium salinarum VPKM B-9451 isolated by step-by-step selection of microorganism strain Halobacterium salinarum VPKM B-9025. Proposed strain is characterized by enhanced level of synthesis of bacteriorhodopsin. Synthesis of bacteriorhodopsin is estimated to be 50-112 mg/l of cultural fluid.
Method for detecting antagonistic properties with respect to pathogenic mycobacterium in lactic acid microorganisms and microorganisms of escherichia coli group / 2320724
Method involves culturing an indicator strain on solid nutrient medium and the following estimation of antagonistic activity. Analyzed strain is cultured in combination with mycobacterial strain on Soton's medium followed by preparing the cultural fluid sterile filtrate. Filtrated is stratified on the solid nutrient medium surface before seeding an indicator strain. Antagonistic activity is evaluated by the ratio of amount of indicator strain colonies on solid nutrient medium to the amount of indicator strain colonies on solid nutrient medium with a stratified sterile filtrate of cultural fluid after their culturing analyzed and mycobacterial strains. Method provides detecting antagonistic properties with respect to pathogenic microorganisms in lactic acid microorganisms and microorganisms of Escherichia coli group.
Solid nutrient medium for mycobacterium culturing / 2320716
Invention relates to a nutrient medium containing potassium hydrogen phosphate, magnesium sulfate, L-asparagine, glycerol, citric acid, iron ammonium citrate, distilled water, sodium pyruvate, agar, humivit and chicken yolk egg aqueous solution (1:1). Invention allows decreasing cost of the nutrient medium and to simplify carrying out bacteriological researches. Invention can be used in investigation of mycobacterium tuberculosis.
 Method for selection of petroleum-oxidizing microorganisms as producers of biosurfactants / 2320715
Method involves culturing petroleum-oxidizing microorganisms on solid medium in Petri dishes at room temperature. In termination of culturing morphotype of microorganism colonies is determined. Microorganism colonies of M-type are selected as producers of biosurfactants. Microorganism colonies of R- and S-type are washed out with phosphate buffer, pH 6.8, and a prepared suspension is diluted to optical value density 0.1-0.3 at wavelength 670 nm followed by assay of emulsifying activity and index of cell hydrophobicity. Microorganisms as producers of biosurfactants are selected at hydrophobicity index value above 20% and the positive emulsifying activity. Method is simple in realization and allows carrying out selection of petroleum-oxidizing microorganisms as producers of extracellular and cell-bound biosurfactants.
Nutrient medium for culturing microorganisms of haemophilus genus / 2320714
Invention proposes a nutrient medium containing aminopeptide, casein hydrolyzate, yeast extract, flax seeds extract, glucose, sodium carbonate, NAD, hemin, sodium chloride, agar and distilled water. Invention provides increasing viability of cells and decreasing polymorphism of cells in smears. Invention can be used in medicine, microbiology and in preparing nutrient medium for culturing microorganisms of Haemophilus genus.
Method for production of biomass from liquid lactobacterium autostrain / 2320355
Claimed method includes sampling of native material, sample seeding on selective nutrient medium without dilution not later than 2 h after sampling; lactobacterium growing and pure culture isolation. Repeated passage is carried out. Obtained pure lactobacterium culture is grown in selective liquid medium followed by addition of conserving agent such as sucrose-gelatin medium in ratio of 1:1 and controlling of obtained liquid biomass.
Biopreparation as oil-destructing agent / 2319740
Invention proposes a biopreparation as an oil-destructing agent that comprises microorganisms culture and microbial fertilizer-carrier as dried biomass of microorganisms from active silt formed in processing waste from swine breeding complexes and containing the following native microorganisms: Bacillus pumilis, Bacillus sphaericus, Micrococcus hylae, Arthrobacter viscous, Bacillus licheniformis. As a culture of microorganisms the strain Bacillus cereus 3K with a titer value 1012 cells/g of carrier is used. Invention provides stimulating the development of oil-oxidizing microflora, increasing the rate of oil decomposition, providing intensification of microbial processes, recovery of agrochemical properties of soil, to combine positive properties of mineral and organic fertilizers, to stimulate development and growth of plants, and to carry out treatment of oil-polluted soils in regions of short heat period.
 Method for production of complex microbiological fertilizer / 2318784
Claimed method includes combination of microbiological component with natural biocompatible carrier, wherein as one part of microbiological component inoculate material of arbuscular mycorrhiza fungi (AM fungi) is used, as natural biocompatible carrier defecate form sugar beet production is used and combination thereof takes place in rizosphere of mycorrhizing plants grown of defecate. Inoculate material of AM fungi is obtained by cultivation of mycorrhized agriculture plants, for example sorgo, Sudan grass, millet, etc. on defecate. Sorgo or other mycorrhized plant seeds are seeded in containers with defecate, pure or mixed culture of AM fungi is introduced therein in form of soil-root mixture in amount of 3-5 g per one seed at occurrence level of mycorrhized roots of at least 60 %. Plants are grown for 90-120 days, then roots with defecate are dried and ground to produce pellety mixture which is blended with adhesive. Then sorgo or other mycorrhized plant seeds are treated with obtained mixture, dried and seeded in defecate and are grown for 90-120 days. Further ground plant parts and upper 20 cm of defecate together with roots are cut, and rest parts of microbiological component such as aqueous suspension of nodule-forming bacteria and/or rizobacteria.
Method for treatment of alfalfa seeds / 2299188
Invention relates to methods used in treatment of alfalfa seeds. Method involves presowing treatment of alfalfa seeds with biopreparation based on the strain Sinorhizobium meliloti Yakutsky №1 GNU VNIIChM 1775 taken in the amount 1 billion of cells per 1 seeds of alfalfa. Method provides enhancing productivity of alfalfa and improving fertility of soils.
Actinomyces fradiae strain for reprocessing of organic waste from animal and poultry breeding / 2298031
Disclosed is Actinomyces fradiae-96 strain isolated from soil sampled form organic poultry breeding waste.
Method for production of peat-grains compost / 2296732
Claimed method includes blending of beer grains with peat in ratio of 1:1 or 1:2 followed bioactivator introducing and aerobic composting at 20-60°C and humidity of 65-70 % for 2-3 months. Compost ferment prepared by culturing of microorganism consortium, isolated from humified humous forest soil layers and containing fungi Aspergillus niger, Aspergillus oryzae, Aspergillus sydowii, Cephalosporium, Glyocladium Cda., Trichoderma sp., actinomycetes Streptomyces griseus, Streptomyces termoviolaceus, Streptomyces globisporus, Streptomyces rubber, Streptomyces viridosporus and bacteria Bacillus cereus, Bacillus mycoides, Bacillus sublilis, in amount of 15 % or more based on composing mixture mass on beer grains is used as bioactivator.
Method for production of fermented agriculture composition / 2296112
Claimed method includes microbial decomposition of plant waste in oxygen-enriched air forced circulation, wherein oxygen is obtained by Chlorella Vulgaris culturing. Carbon dioxide released in process of plant waste decomposition is collected and used in algae culturing. Method also includes manure deodorizing with calcium oxide or calcium hydroxide. Deodorized manure is mixed with mass obtained in process of plant waste decomposition. Mixture is fermented for 4-5 days and mixed with solid fraction of Chlorella Vulgaris. Said solid fraction of Chlorella Vulgaris in obtained by double freezing and defrosting of algae biomass and cell juice separation. Process is carried out in closed cycle. Obtained composition is useful both as fertilizer and feed supplement.
Biopreparation-petroleum destructor / 2292326
Invention relates to a biological preparation used in treatment of soil polluted with petroleum and oil products in simultaneous recovery of its physicochemical properties and natural biocenosis. The biopreparation-petroleum destructor comprises microorganism culture and bacterial fertilizer as fermented bedding avian dung containing the following native microorganisms: Clavibacter michiganese, Bacillus amyloliquefaciens and Micrococcus varians. As a microorganism culture the strain Bacillus cereus 3K is used with a titer value 1012 cells/g of bacterial fertilizer. Invention provides enhancing petroleum-destructing activity, to simulate development of petroleum-oxidizing microflora, to increase the rate of petroleum decomposition, to provide intensification of bacterial processes recovering agrochemical properties of soil, to carry out treatment of petroleum-polluted soils in regions with short heat period.
 Hyperthermophile strain caldothrix satsumae capable of fermentation of organic waste at high temperatures / 2291900
Strain Caldothrix satsumae YM081 is isolated from compost by fermentation of organic waste at 85°C or higher. Strain is deposited under number FERM BP-8233.
 Organic nitrogen-containing composition and fertilizer comprising thereof / 2291139
Invention proposes an organic nitrogen-containing composition comprising enzymatic mother solution prepared by culturing microorganism of genus Enterobacter that is able to produce L-glutamic acid in liquid medium. Culturing is carried out at pH value providing precipitation of L-glutamic acid under condition that L-glutamic acid is produced and accumulated with accompanying precipitation and the following separation of L-glutamic acid from medium. Microorganism can metabolize carbon source in liquid medium containing L-glutamic acid in the saturation concentration and carbon source at the definite pH value and possesses capacity to accumulate L-glutamic acid in the amount exceeding the saturating concentration of L-glutamic acid in liquid medium at this pH value. Prepared composition is used as a component of fertilizer. The claimed invention provides expanding assortment of fertilizers.
Method for compost production from organic waste / 2290390
Claimed method includes waste mixing with paunch mature as microelement source and baking or beer yeast or yeast extracts and autolysates. Mixture is moistened up to humidity of 30-50 % and composted under aerobic conditions, wherein carbon/nitrogen ratio in finished product is from 10 to 50.
Method for reprocessing of organic waste by composting with worms / 2290389
Claimed method includes preliminary composting of organic waste by introducing of paunch mature as microelement source and yeast or yeast extracts as nitrogen source. Organic waste with additives is agitated for 7-12 days to produce unripe compost. Unripe compost is mixed with waste from pulp-and paper or wood processing industry and additional nitrogen sources, molasses and earthworms. In process of composting with worms enzyme activity of composting mixture is controlled.
Biological fertilizer / 2249584
Biological fertilizer contains (%): growth agents (e.g., aqueous extract from Kalanchoe leaves) 3; inhibitor of soil pest growth and fungal and bacterial diseases (e.g., soil-entomopatogenic associative nematodes and waste products thereof, as well as living nematode-symbiotic bacteria) 0.001 and 0.0001, respectively; feed source (e.g., sodium humate) 0.1; and balance: water. Fertilizer of present invention finds plants with feedstuff, physiologically active substances and reduces helpful symbiotic microflora of rhizosphere.
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FIELD: biotechnological production of fertilizers. SUBSTANCE: method envisages mixing bird dung with moisture-absorbing material followed by aerobic fermentation of the mixture in presence of microorganisms. Process is carried out at stirring until temperature of fermentation mixture spontaneously drops to 25-30°C, said microorganisms being consortium of strains: Bacillus subtilis B-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-9-7, Candida tropicalis Y-1520, Candida utilis Y-2441. Preferable implementation of the method is when above-listed strains are used in equal proportions and in amount of 1•108-1•109 cells in 1 mL per 1 ton bird dung. EFFECT: enabled production of less expensive high-efficiency fertilizer due to reduced processing expenses because of simplified technology and diminished use of consortium of strains. 2 cl
The invention relates to the bioconversion of poultry farms and can be used for environmentally friendly effective fertilizer for agricultural crops. The creation of large poultry farms on an industrial basis cause significant disruption to the ecological balance, environmental pollution areas of the farm and surrounding areas, and airspace, as when stored in pure form litter quickly compressed and emits a fetid odor caused by the release of decomposition products. In the litter develop pathogenic microflora and helminth eggs. The number of known methods of disposal of manure and poultry litter: methane fermentation for biogas production; - long composting for organic fertilizers; - chemical treatment litter; thermal drying of the litter at different temperatures; - processing of litter insects and worms; - microbiological conversion of manure and litter. There is a method of biological treatment of bird droppings, providing mixing it with a water-absorbing material and the subsequent blowing oxygen-containing gas, in which the fermentation process carried out by microorganisms, the original being in SIP is those for 70-90 hours and before loading litter with moisture-absorbing material is heated, see SU inventor's certificate No. 1749217, IPC C05F 3/00, 1992. The disadvantage of this method is that the implementation of this technology requires a special reactor design, where the purge gas mixture in the longitudinal and transverse directions during the period of fermentation and before loading into the reactor, the mixture is heated special copper electrode. The closest in technical essence is a method of biological treatment of bird droppings, providing a mixture of poultry manure with a moisture-absorbing material with subsequent aerobic fermentation of the mixture in the presence of a consortium of microorganisms, microorganisms use (Streptococcus fhermophilus. Streptococcus bovis, Lactobacillus salivarius var. sallcinicus, Lactobacillus salivarius var salivarius, Lactobacillus acophilus) No. VKPM B-5972 that contribute to litter before it is mixed with water-absorbing material, and pre-bird droppings in the presence of a consortium of bacteria are fermented with natural conditions, and then the resulting mixture is subjected to aerobic fermentation under stirring in the presence of synanthropic flies to a natural reduction of the temperature of the fermentation mixture to 25-30°and then again in the mix make the specified consortium of bacteria and fermented it in natural conditions is the second. Primary execution method, when the consortium contribute to litter in an amount of 0.01 to 4.0%, and in fermentive mixture of 0.01 to 8.0%, see RU Patent No. 2055823, IPC C05F 11/08, 1996 The disadvantage of this method is the complexity of the technological process, as well as high consumption of bacteria of the genus Streptococcus and Lactobacillus. The task of the invention is to simplify the process, the reduction of the insertion of the consortium. The technical problem is solved by a method of biological treatment of bird droppings, providing a mixture of poultry manure with a moisture-absorbing material with subsequent aerobic fermentation of the mixture in the presence of microorganisms with stirring to a natural reduction of the temperature of the fermentation mixture to 25-30°where as microorganisms use the consortium strains of Bacillus subtilis b-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y-2441. Primary execution method of biological treatment of bird droppings, when a consortium of strains of Bacillus subtilis b-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y-2441 used in quantities of 1·108-1·109cells in 1 ml of 1 t bird droppings. Technical solution allows to simplify the method of biological treatment of poultry manure by reducing the number t is geologicheskikh operations and reduce insertion consortium. Examples of specific performance. An example of a specific implementation of the prototype. In litter humidity of 80-90%, which was in the lagoon of the house, make a liquid culture of a consortium of bacteria (Streptococcus fhermophilus, Streptococcus bovis, Lactobacillus salivarius var. sallcinicus, Lactobacillus salivarius var salivarius, Lactobacillus acophilus) No. VKPM B-5972 to a concentration of 0.01 to 4.0%. Pre-bird droppings in the presence of a consortium of bacteria are fermented with natural conditions within 3-5 days. Then this litter is moved into the trench and mix with water-absorbing material - solid manure or peat. The resulting mixture at a temperature of 60-80°subjected to aerobic fermentation, aeration fermentive litter carried out with mechanical stirring and with larvae of synanthropic flies. The fermentation is carried out in a period of 6-8 days to a natural reduction of the temperature of the fermentation mixture to 25-30°With. then again make a liquid culture of a consortium of bacteria No. VKPM B-5972 in an amount of 0.01 to 8.0% and is uniformly mixed with the augers, fermenting are 2 days at a temperature of 20°C. Examples 1-4 according to the claimed object. Example 1. Litter with a humidity of 90% is mixed with water-absorbing material, as the latter is used bedding litter with a humidity of 50%. In the mixture contribute lop is the FMD strains of Bacillus subtilis b-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y-2441 1·108cells in 1 ml per 1 ton of poultry litter by means of spraying throughout the volume of the pile, and then lead the aerobic fermentation of the mixture under stirring and in the absence of ultraviolet rays to naturally reducing the temperature of the fermentation mixture to 25-35°C. the resulting organic fertilizer is Packed in plastic containers and stored. The resulting product can also be pelletized with subsequent drying. Example 2. Litter humidity 96% is mixed with water-absorbing material, as the latter is used sawdust. In the mixture contribute consortium of strains of Bacillus subtilis B-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y-2441 1·108cells in 1 ml per 1 ton of poultry litter by means of spraying throughout the volume of the pile, and then lead the aerobic fermentation of the mixture under stirring and in the absence of ultraviolet rays to naturally reducing the temperature of the fermentation mixture to 25-35°C. the resulting organic fertilizer is Packed in plastic containers and stored. The resulting product can also be pelletized with subsequent drying. Example 3. Litter humidity 96% is mixed with water-absorbing material, as the latter is used torff the mixture contribute consortium of strains of Bacillus subtilis B-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y-2441 1·108cells in 1 ml per 1 ton of poultry litter by means of spraying throughout the volume of the pile, and then lead the aerobic fermentation of the mixture under stirring and in the absence of ultraviolet rays to naturally reducing the temperature of the fermentation mixture to 25-35°C. the resulting organic fertilizer is Packed in plastic containers and stored. The resulting product can also be pelletized with subsequent drying. Example 4. In litter with 90% humidity contribute consortium of strains of Bacillus subtilis B-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y-2441 1·109cells in 1 ml per 1 ton of poultry litter by means of spraying throughout the volume of the pile, and then are mixed with water-absorbing material, as the latter is used bedding litter with a humidity of 50%, and then lead the aerobic fermentation of the mixture under stirring and in the absence of ultraviolet rays to naturally reducing the temperature of the fermentation mixture to 25-35°C. the resulting organic fertilizer is Packed in plastic containers and stored. The resulting product can be pelletized with subsequent drying. Thus obtained product of gray can be used as an effective b is odobreniya. The product has the following quality indicators, % (on dry matter):
The obtained product with these characteristics can be used as an effective fertilizer. In industrial conditions in the pre-sowing application of organic fertilizers by the present method obtained significantly higher yields. Data on the results of the experiments 2005 Board some cultures are shown in table 2:
Based on the positive results of the studies were conducted in an industrial environment with spring wheat Luba by the preplant treatment field area of 750 hectares in the amount of 3-4 t/ha of organic fertilizer obtained by the present object. The increase wheat yield was 46% compared with the control field (without fertilizers). In industrial conditions at pre-treatment field area of 550 ha in the amount of 3-4 t/ha of organic fertilizer obtained by the present object, the yield increase of barley BIOS-1 was 27% compared with the control field. In addition, the fertilizer can be used as a top dressing in the spring, summer and autumn as when digging the soil, and by irrigation. When freezing and thawing fertilizer retains its properties, shelf life is unlimited. The strains belonging to the consortium of microorganisms: Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y-2441, see the all-Union collection of microorganisms, Bacillus subtilis b-168, see the Collection of Cultures of Microorganisms, State Reseach Center of Virology and Biotechnology "Vector". Strains consortium cultivated on sterile nutrient medium required for each type of strain, and in the right quantity. Of these in equal shares strains of microorganisms prepared by the consortium for industrial use. Fertilizer contains all the necessary plant nutrients stimulate their development of organic substances and trace elements, which enriches the soil structure, increases its fertility for the long term (up to 3 years), increases the resistance of plants to bacterial and fungal diseases (late blight, scab, root rot, Fusarium and others), prevents acidification of the soil, optimizes moisture supply, eliminates the appearance on the surface of the mold, non-caking, non-toxic, in contact with skin no harmful impact on the body. Thus, the inventive method allows you to get cheaper high-efficiency fertilizer by reducing the cost of its processing by simplifying the technological process and reduce the consumption of insertion consortium of strains. 1. The method of biological treatment of bird droppings, providing a mixture of poultry manure with a moisture-absorbing material with subsequent aerobic fermentation in the presence of microorganisms with stirring to a natural reduction of the temperature of the fermentation mixture to 25-30°C, characterized in that the microorganisms use the consortium strains of Bacillus subtilis b-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y-2441. 2. The method according to claim 1, characterized in that the strains of Bacillus subtilis b-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-907, Candida tropicalis Y-1520, Candida utilis Y-2441 used in equal proportions and number 1·108-1·109cells in 1 ml of 1 t bird droppings.
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