Actinomyces fradiae strain for reprocessing of organic waste from animal and poultry breeding |
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IPC classes for russian patent Actinomyces fradiae strain for reprocessing of organic waste from animal and poultry breeding (RU 2298031):
Method for production of androsta-1,4-diene-3,17-dione from plant and animal derived sterols / 2297455
Invention relates to microbial production of androsta-1,4-diene-3,17-dione (ADD). Claimed method is based on ability of specific Mycobacterium neoaurum bacteria strain to selectively eliminate side chain of abovementioned sterols and to accumulate ADD in presence of transformation product sorbent. As sorbents synthetic polymer resins obtained by copolymerization of divinylbenzene and ethylstyrene are used. Sorbents are added in broth in end of lag-phase. ADD is formed with yield of 66-76 % depending on side chain structure of transformed substrates.
 Polypeptides derived from tryptophanyl-trna-synthetase and uses thereof in controlling of vascularisation / 2297425
Invention relates to water soluble polypeptides (SEQ ID NO.12) and (SEQ ID NO.7), derived from full-length tryptophanyl-tRNA-synthetase and having angiostatic activity in relation to eye neovasculatisation. Also disclosed are polynucleotides, encoding truncated polypeptide forms (SEQ ID NO.12) and (SEQ ID NO.7), and E.coli cell, expressing abovementioned polypeptides. Said polypeptides are useful in injecting angiostatic composition and kit for inhibiting of eye neovasculatisation.
Bifidum bacterium strain consortium to produce sour-milk, fermented and non-fermented foodstuffs, ferments, bioactive supplements, bacterium preparations, and cosmetic agents / 2296797
Claimed consortium is produced by combination of four bifidum bacterium strains, namely B.bifidum 79-37, B.longum, B.breve 79-88, B.infantis 79-43. Said consortium actively propagates in nutritional media with biomass accumulation in short culturing time; ferments milk with clot production having good taste and organoleptic characteristics; and has specific activity. Consortium is capable of human intestinal microbial population normalizing in short time and is useful in production of healthy products with good organoleptic characteristics.
Method for production of peat-grains compost / 2296732
Claimed method includes blending of beer grains with peat in ratio of 1:1 or 1:2 followed bioactivator introducing and aerobic composting at 20-60°C and humidity of 65-70 % for 2-3 months. Compost ferment prepared by culturing of microorganism consortium, isolated from humified humous forest soil layers and containing fungi Aspergillus niger, Aspergillus oryzae, Aspergillus sydowii, Cephalosporium, Glyocladium Cda., Trichoderma sp., actinomycetes Streptomyces griseus, Streptomyces termoviolaceus, Streptomyces globisporus, Streptomyces rubber, Streptomyces viridosporus and bacteria Bacillus cereus, Bacillus mycoides, Bacillus sublilis, in amount of 15 % or more based on composing mixture mass on beer grains is used as bioactivator.
Consortium of bifidobacterium strains to prepare lactic fermented and non-fermented food products, biologically active additives, leavens, bacterial preparations, hygienic agents, and cosmetics / 2296158
Invention relates to food processing, cosmetic, biotechnological, and medicinal industries. Consortium is prepared by way of combining eight bifidobacterium strains: B. bifidum 79-37, LVA-3, B. Longum V79M, Ya-3, Ya-4, B. breve 79-88, B. Unfantis 79-73, B. adolescentis GO-13. Proposed eight-strain consortium is grown on nutrient media to accumulate production mass within short culturing terms, ferments milk to form clot having good flavor and organoleptic properties, and possesses specific activity. Eight-strain consortium showing high specific activity, is capable of rapidly ecizing in human bowels and promotes normalization of gastric microflora within short periods of time. Consortium may also be a source of therapeutical and prophylactic food products having good organoleptic properties.
Consortium of bifidobacterium strains to prepare lactic fermented and non-fermented food products, leavens, biologically active additives, bacterial preparations, hygienic agents, and cosmetics / 2296157
Invention relates to food processing, cosmetic, biotechnological, and medicinal industries. Consortium is prepared by way of combining four bifidobacterium strains: Bifidobacterium bifidum 79-37, LBA-3, B. longum B379M, and Ya-4. Consortium is actively grown on nutrient media to accumulate production mass within short culturing terms, ferments milk to form clot having good flavor and organoleptic properties, and possesses specific activity. Four-strain consortium, showing high specific activity, is capable of rapidly ecizing in human bowels and promotes normalization of gastric microflora within short periods of time. Consortium may also be a source of therapeutical and prophylactic food products having good organoleptic properties.
 Strain bifidobacterium lactic 672 used to prepare lactic therapeutic and prophylactic products, fermented and non-fermented food products, biologically active additives, probiotic, and cosmetics / 2296156
Invention is intended for use in, food processing, cosmetics, biotechnological, medicinal industries, and veterinary in order to strengthen health, in particular when preparing lactic fermented and non-fermented food products, cosmetics, biologically active additives, and probiotic. Strain Bifidobacterium lactis 672 BKPM As-1677 is isolated from contents of healthy baby bowels. Invention enhances biological activity of the strain and its resistance to environmental conditions, accelerates growth on artificial nutrient media and acid-forming and antagonistic activity with regard to pathogenic and conditionally pathogenic microflora. Preparation of strains possessing above properties promotes extension of assortment of probiotics used to maintain health, in particular to normalize microflora gastroenteric and urogenital tracts, human and animal cutaneous coverings and mucous membranes.
 Bacterium strain borrelia garinii bgvir-1 for preparation of drugs for diagnosis and prophylaxis of ixodic mite-born borreliosis and for evaluation of protective and specific activity of prophylaxis and therapeutic agents / 2295564
Bacterium strain Borrelia garinii BgVir-1 synthesizes all basic immunogenic proteins of borrelia from group B. burgdorferi s.L and is useful in production of diagnostic preparations and vaccine.
 Bacterium strain lactococcus lactis subcpecies lactis b-8558 useful in dairy product preparation and method for production of starter culture of strain lactococcus lactis subcpecies lactis b-8558 / 2295563
Invention relates to new natural strain of lactic acid bacteria capable of exopolysaccharide synthesis in amount sufficient to improve rheologic product characteristics without introducing of food supplement and to increase functional properties of products producing with said strain. Starter culture of claimed strain is produced by fermentation under condition of semi-continuous deep culturing on broth containing milky whey, concentrated hydrolyzed milk, KH2PO4, Na2HPO4, MgSO4, sodium citrate, and distilled water. Fermentation is carried out at 22-32°C, constant pH 6.0-7.0 up to accumulation of living cell amount of 8.4-9.5 lg CFU/cm3. Biomass is separated, resuspended in protective medium, wherein as cryoprotector defatted milk with increased mass part of dry matter (up to 15 %) or defatted milk blended with gelatin in equal ratio is used; frozen and lyophilized. After drying biomass in bottles is sealed or pre-packed in sealed packing materials.
Bacterium strain bacillus spp. kr-083 as plant protection agent against phytopathogenic microorganism and for growth stimulation thereof / 2295562
Disclosed is new strain of rhizosphere bacteria bacillus spp. KR-083 as plant protection agent against phytopathogenic microorganism and for growth stimulation thereof. Claimed strain is isolated from South Corey rice roots.
Method for production of peat-grains compost / 2296732
Claimed method includes blending of beer grains with peat in ratio of 1:1 or 1:2 followed bioactivator introducing and aerobic composting at 20-60°C and humidity of 65-70 % for 2-3 months. Compost ferment prepared by culturing of microorganism consortium, isolated from humified humous forest soil layers and containing fungi Aspergillus niger, Aspergillus oryzae, Aspergillus sydowii, Cephalosporium, Glyocladium Cda., Trichoderma sp., actinomycetes Streptomyces griseus, Streptomyces termoviolaceus, Streptomyces globisporus, Streptomyces rubber, Streptomyces viridosporus and bacteria Bacillus cereus, Bacillus mycoides, Bacillus sublilis, in amount of 15 % or more based on composing mixture mass on beer grains is used as bioactivator.
Method for production of fermented agriculture composition / 2296112
Claimed method includes microbial decomposition of plant waste in oxygen-enriched air forced circulation, wherein oxygen is obtained by Chlorella Vulgaris culturing. Carbon dioxide released in process of plant waste decomposition is collected and used in algae culturing. Method also includes manure deodorizing with calcium oxide or calcium hydroxide. Deodorized manure is mixed with mass obtained in process of plant waste decomposition. Mixture is fermented for 4-5 days and mixed with solid fraction of Chlorella Vulgaris. Said solid fraction of Chlorella Vulgaris in obtained by double freezing and defrosting of algae biomass and cell juice separation. Process is carried out in closed cycle. Obtained composition is useful both as fertilizer and feed supplement.
Biopreparation-petroleum destructor / 2292326
Invention relates to a biological preparation used in treatment of soil polluted with petroleum and oil products in simultaneous recovery of its physicochemical properties and natural biocenosis. The biopreparation-petroleum destructor comprises microorganism culture and bacterial fertilizer as fermented bedding avian dung containing the following native microorganisms: Clavibacter michiganese, Bacillus amyloliquefaciens and Micrococcus varians. As a microorganism culture the strain Bacillus cereus 3K is used with a titer value 1012 cells/g of bacterial fertilizer. Invention provides enhancing petroleum-destructing activity, to simulate development of petroleum-oxidizing microflora, to increase the rate of petroleum decomposition, to provide intensification of bacterial processes recovering agrochemical properties of soil, to carry out treatment of petroleum-polluted soils in regions with short heat period.
 Hyperthermophile strain caldothrix satsumae capable of fermentation of organic waste at high temperatures / 2291900
Strain Caldothrix satsumae YM081 is isolated from compost by fermentation of organic waste at 85°C or higher. Strain is deposited under number FERM BP-8233.
 Organic nitrogen-containing composition and fertilizer comprising thereof / 2291139
Invention proposes an organic nitrogen-containing composition comprising enzymatic mother solution prepared by culturing microorganism of genus Enterobacter that is able to produce L-glutamic acid in liquid medium. Culturing is carried out at pH value providing precipitation of L-glutamic acid under condition that L-glutamic acid is produced and accumulated with accompanying precipitation and the following separation of L-glutamic acid from medium. Microorganism can metabolize carbon source in liquid medium containing L-glutamic acid in the saturation concentration and carbon source at the definite pH value and possesses capacity to accumulate L-glutamic acid in the amount exceeding the saturating concentration of L-glutamic acid in liquid medium at this pH value. Prepared composition is used as a component of fertilizer. The claimed invention provides expanding assortment of fertilizers.
Method for compost production from organic waste / 2290390
Claimed method includes waste mixing with paunch mature as microelement source and baking or beer yeast or yeast extracts and autolysates. Mixture is moistened up to humidity of 30-50 % and composted under aerobic conditions, wherein carbon/nitrogen ratio in finished product is from 10 to 50.
Method for reprocessing of organic waste by composting with worms / 2290389
Claimed method includes preliminary composting of organic waste by introducing of paunch mature as microelement source and yeast or yeast extracts as nitrogen source. Organic waste with additives is agitated for 7-12 days to produce unripe compost. Unripe compost is mixed with waste from pulp-and paper or wood processing industry and additional nitrogen sources, molasses and earthworms. In process of composting with worms enzyme activity of composting mixture is controlled.
Method for preparing biohumus enriched with phosphorolytic microorganisms / 2286983
Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Agricultural animal manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting process is carried out for 2-3 months at temperature 16-32°C under natural conditions, and after separation of worms from biohumus microorganisms of species Bacillus megaterium var. phosphaticum are added to biohumus in the concentration 1010-1012 cells per 1 kg of biohumus. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity, decreasing material consumptions and decreasing time for preparing the preparation.
Method for preparing biohumus-base bacterial fertilizer / 2286982
Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Agricultural animal manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting process is carried out for 2-3 months at temperature 16-32°C under natural conditions, and after separation of worms from biohumus on vibrating screen with pores size 0.5-1.0 cm then biohumus is dried up to moisture 50-60%, packaged into polypropylene packets, molasses is added in the amount 1.0-3.0% of the biohumus mass, subjected for autoclaving at 0.8-1.2 atm for 45-75 min followed by addition of microorganisms of species Rhizobium japonicum. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity, decreasing material consumptions and decreasing time for preparing the preparation.
Method for preparing biohumus-base combined fertilizing soil / 2286981
Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Agricultural animal manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting process is carried out for 2-3 months at temperature 16-32°C under natural conditions, and after separation of worms from biohumus the strain Bacillus megaterium var. phosphaticum and the strain Agrobacterium radiobacter are added. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity, decreasing material consumptions and decreasing time for preparing the preparation.
Biological fertilizer / 2249584
Biological fertilizer contains (%): growth agents (e.g., aqueous extract from Kalanchoe leaves) 3; inhibitor of soil pest growth and fungal and bacterial diseases (e.g., soil-entomopatogenic associative nematodes and waste products thereof, as well as living nematode-symbiotic bacteria) 0.001 and 0.0001, respectively; feed source (e.g., sodium humate) 0.1; and balance: water. Fertilizer of present invention finds plants with feedstuff, physiologically active substances and reduces helpful symbiotic microflora of rhizosphere.
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FIELD: biotechnology, veterinary microbiology, sanitary. SUBSTANCE: disclosed is Actinomyces fradiae-96 strain isolated from soil sampled form organic poultry breeding waste. EFFECT: active strain for reprocessing of organic waste from animal and poultry breeding. 2 ex
The invention relates to the field of environmental protection, veterinary Microbiology, sanitation and biotechnology and can be used in the processing of organic waste agricultural enterprises with the simultaneous production of ecologically clean and highly effective fertilizer. The use of this strain allows to provide year-round recycling organic waste with the lowest production costs. The aim of the invention is a strain of Actinomyces fradiae-96 designed for recycling of different kinds of organic materials, including human excreta, agricultural animals and birds. Analogs of the invention. Developed physical, chemical and biological recycling of manure and litter with coprophagous, yeast, microorganisms, etc.) methods of processing organic materials (see Nikitin D.P. 1980, Voroshilov SCI, Durdyev S.D., erbanova L.N., 1991; Lysenko V.P. 2002; Blinov, VA, 2003). However, most of the known methods have several disadvantages, both from an economic and from a biological point of view. For example, some of them expensive and therefore inaccessible to most agricultural enterprises, other effective only under certain climatic conditions. The most appropriate and effective for the treatment of organic cheese which I is the use of specific populations of microorganisms and fungi. It is known that microorganisms contribute to the accumulation in soil of plant nutrients, stimulate their growth and development, have antagonistic activity against zoo and phytopathogens. For processing of organic waste are methane-producing, thermophilic, photosynthetic and lactic acid bacteria, yeast, fermenting fungi. Proceeding from this, in the examples discussed ways of recycling organic wastes using microorganisms and fungi. So there is a method of recycling organic waste with the addition of methane-forming bacteria and the subsequent introduction of a yeast starter culture (No. 1625859). The disadvantage of this method is the low nutritional value of the resulting fertilizer and labor intensive process. In another method for disinfection of poultry litter was used bacterial strain Bacillus subtilis CG-3. However, the main disadvantage of using Bacillus subtilis strain CG-3 is the length of the process (90 days). The closest in technical essence and the achieved result of the claimed invention is the technology of effective microorganisms (see pancakes VA Biotechnology. Saratov. - 2003. - 196 C.). Created on the basis of EM-technology drugs such as "SEF-A", "Baikal", "Tamir", "Kussa EM-1", "Tank NIB" and other effective is used for the processing of domestic and agricultural wastes. However, the use in the processing of organic waste effective microorganisms (photosynthetic and lactic acid bacteria, yeast, actinomyces) has drawbacks, in particular the impossibility year-round to perform processing, the need to cover the substrate, the high price of drugs, which makes a broad introduction to the practice and implementation among the population. The proposed method allows to reduce time of processing of organic raw materials, to conduct reliable neutralization of pathogens and helminths, to eliminate environmental pollution. Moreover, the process of organic wastes is carried out with minimal production costs. An important advantage of the inventive object is that the bioconversion of organic waste this strain does not depend on the temperature conditions. The strain deposited 10.08.2004 year FGI VGNKI no 04.05.17-DEPT (Actinomyces fradiae-96). Certificate of Deposit issued strains for # 2. The strain of Actinomyces fradiae-96 identified by the authors in 1996 from soil taken from under organic waste poultry at the poultry farm "Pestrechinskaya" Petrichenkova district of the Republic of Tatarstan, and after studying its useful properties deposited in Moscow, all-Russian research veterinary Institute" (Kazan), number 2/2004. Hara is the statistics of strain not previously published. Strain for other purposes not used. Actinomyces fradiae-96 has the following morphological and physiological characteristics. The morphology. Cells oval; length 0,006±0,0014 mm; width of 0.004±0,0005 mm single-Celled mycelium of branching, thin hyphae. The micromycetes stain gram-positive. Cultural characteristics. Liquid nutrient medium (BCH) after 24-48 hours of cultivation there is a uniform clouding with the formation of wrinkled mucous membrane. Biochemical properties. Strain aerobe, although growth and limited access of oxygen. Has expressed sacharolytica activity intensively (on the environment gissa) fermented with production of acid without gas - glucose, lactose, sucrose, mannitol, sorbitol, glycerin, with the formation of acid and gas is observed cleavage of maltose. The strain does not break rhamnose, raffinose and dulcet. Has catalase and urease activity, liquefies gelatin, does not form a hydrogen sulfide and indole. Physiological signs. Reproduction by means of aerial hyphae, it is also possible by means of fragmentation. The harmless strains studied in the test - dose (500 mg) in mice; this was administered to a suspension of micromycete in physiological solution two groups of animals (6 goals) inside. The strain is not pathogenic for warm-blooded animals, because n is celebrated the death of mice and visible deviations from the physiological state. The proposed method of disposal of organic waste is as follows. Organic waste (excrement of various types of farm animals, birds) layers treated with an aqueous suspension of the microorganism rate of 20 ml of native culture per 1 ton of the substrate, containing 1 ml of at least 200 million a MIC. class. Then the resulting mass is subjected to composting within 30 days in the warm season and within 40-60 days in the cold. Strain micromycete has pronounced activity, effectively decompose the excrement of birds and animals, turning them into high-quality humus. The proposed method is illustrated by examples of specific applications. Example 1. The experiments were conducted under conditions of cattle and pig farms. On a solid platform laid manure (height 1 m). This surface was evenly sprayed with an aqueous suspension of the microorganism rate of 20 ml of native culture per 1 ton of the substrate, containing 1 ml of at least 200 million a MIC. class. Then the surface of the newly laid substrate and repeated the procedure. The entire fermentation process was taken in the summer - 30; winter - 40-60 days. Samples for microbiological and microscopic analysis were taken at the beginning of treatment (original mix) and every 10 days after fermentation. The presence in the samples of microo the of organisms, protozoa and helminths was assessed by conventional methods. The results of microbiological and microscopic analysis showed the complete disinfection of pathogens and helminths. Example 2. Experiments carried out at the poultry farms in several regions of the Russian Federation. To eliminate liquid consistency Pametnik masses were used sawdust. On the ground with a hard surface laid litter and sawdust layers up to a height of 1.5 m, each layer watered with an aqueous suspension of the microorganism rate of 20 ml of native culture per 1 ton of the substrate, containing 1 ml of at least 200 million a MIC. class. the fermentation Process in the summer period amounted to 30, winter is 40-60 days. At the beginning of treatment (original mix) and every 10 days after fermentation samples were collected substrate. In samples were determined by microbiological contamination of pathogenic and conditionally pathogenic microflora, the presence of helminth eggs and protozoa. The results of microbiological and microscopic analysis showed the complete disinfection of pathogens and helminths. Sources of information 1. Copyright certificate №2105810, CL 05F 3/00, 11/08. 2. Copyright certificate №1625859, CL 05F 3/00, 91. 3. Pancakes, VA Biotechnology. - Saratov. - 2003. - 196 S. 4. Nikitin D.P. Large cattle-breeding complexes and the physical environment. - Medicine. - Moscow. - 1980. - 255 S. 5. Lysenko VP advanced technologies and equipment for reconstruction and technical re-equipment in the poultry industry. - Moscow. - FGNU "Rosinformugol". - 2002. - S-453. 6. Voroshilov SCI, Durdyev S.D., erbanova LN. Livestock facilities and environmental protection. - Moscow. - Agropromizdat. - 1991. - 107 C. The strain of Actinomyces fradiae VGNKI 04.05.17-DEPT used for processing organic wastes of livestock and poultry.
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