Biopreparation-petroleum destructor |
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IPC classes for russian patent Biopreparation-petroleum destructor (RU 2292326):
 Hyperthermophile strain caldothrix satsumae capable of fermentation of organic waste at high temperatures / 2291900
Strain Caldothrix satsumae YM081 is isolated from compost by fermentation of organic waste at 85°C or higher. Strain is deposited under number FERM BP-8233.
 Microorganism composition (variants) / 2291899
Claimed composition contains Lactobaccilus rhamnosus LGG (ATCC 53103), Lactobaccilus rhamnosus LC705 (DSM 7061), propionibacterium freudereichii ssp. Shermanii PJS (DSM 7067) and bifidobacterium, preferably Bifidobacterium infantis Bbi99 (DSM 13692). Abovementioned compositions are useful in food processing industry, pharmaceutical industry, production of healthy products.
 Consortium of bifidobacterium strains used in preparing liquid concentrate of bifidobacterium, fermented-milk, nonfermented, curative-prophylactic foodstuffs, biologically active supplements and bacterial preparations / 2291194
Invention represents a consortium of strains of bifidobacteria. The consortium comprises the following strains of bifidobacteria: Bifidobacterium bifidum № 791 BAG, Bifidobacterium bifidum 8-3, Bifidobacterium longum DVA-13 used in preparing a liquid concentrate of bifidobacteria, fermented-milk, nonfermented curative-prophylactic foodstuffs, biologically active supplements and bacterial preparations. The ratio of strains in the consortium is 8:1:1, respectively. The consortium of strains of bifidobacteria shows enhanced resistance against damaging factors of cultured media and aggressive media of human gastrointestinal digestive tract that provides utilization of broader spectrum of amino acids, i. e. it possesses higher viability and colonizing properties that, in turn, provides preparing preparations and foodstuffs based on thereof showing prolonged storage times in retaining high curative-prophylactic properties. The proposed consortium strains of bifidobacteria can be used in preparing liquid concentrate of bifidobacteria and in preparing fermented-milk, nonfermented curative-dietetic foodstuffs, bifido-containing biologically active supplements and preparation-probiotics based on the indicated liquid concentrate of bifidobacteria.
![Method for production of 4-[4-[4-(hydroxybiphenylmethyl)-1-piperidinyl]-1-hydroxybutyl]- α,α-dimethylphenylacetic acid](http://img.russianpatents.com/img_data/122/1222359-s.gif) Method for production of 4-[4-[4-(hydroxybiphenylmethyl)-1-piperidinyl]-1-hydroxybutyl]- α,α-dimethylphenylacetic acid / 2290443
4-[4-[4-(hydroxybiphenylmethyl)-1-piperidinyl]-1-hydroxybutyl]-α,α-dimethylphenylacetic acid is produced by incubation of mixture, containing terphenadine and microorganism capable to produce said acid followed by isolation of target product.
 Strain bifidobacterium lactis 678 useful in preparation of fermented-milk products, healthy products, fermented and non-fermented foodstuffs, bioactive supplements, probiotics, and cosmetic agents / 2290437
Strain Bifidobacterium lactis 678 is isolated from bulk of healthful infant and is characterized with increased biological activity, environmental strength, accelerated propagation on artificial cultural media, acid-forming and antagonistic activity against pathogenic and opportunistic microorganisms. Strain is useful in preparation of functional foodstuff, bioactive supplement, cosmetic and toiletry agents.
 Strain bifidobacterium lactis 676 useful in preparation of fermented-milk products, healthy products, fermented and non-fermented foodstuffs, bioactive supplements, probiotics, and cosmetic agents / 2290436
Strain Bifidobacterium lactis 676 is isolated from bulk of healthful infant and is characterized with increased biological activity, environmental strength, accelerated propagation on artificial cultural media, acid-forming and antagonistic activity against pathogenic and opportunistic microorganisms. Strain is useful in preparation of functional foodstuff, bioactive supplement, cosmetic and toiletry agents.
Enzyme for production of fermented milk product / 2290435
Claimed ferment contains bacterium strains mixture of Lactococcus lactis BKM B-2232D, Lactobacillus helveticus BKM B-842, Lactobacillus acidophilus BKM B-1660, Streptococcus thermophilus BKM B-2237D, Propionibacterium freudenreichii subspm shermanii BKM B-2233D. Fermented milk product obtained by using of this ferment is effective in treatment of gastrointestinal diseases.
 Dry diagnostic broth for identification of malignant anthrax exitor / 2289622
Claimed broth contains as nutritional base enzymetic casein hydrolysate, sodium chloride, potassium chloride, 12-hydrous sodium phosphate, agar-agar, polymixine sulfate, and sodium hydrocarbonate in specific ratio.
Bacteria strain bacillus mucilaginosus having wide spectrum of fungicide action and biopreparation based on the same / 2289621
Strain Bacillus mucilaginosus Bac-10 is isolated from cultured soils. Biological preparation obtained on the base of abovementioned strain is useful in increasing of productivity of agriculture cultures and increasing resistance thereof to various diseases. Claimed preparation may be used in combination with pesticides.
Bacteria strain azotobacter chrococcum having wide spectrum of fungicide action and biopreparation based on the same / 2289620
Strain Azotobacter chrococcum BH-1811 is isolated from cultured soils. Biological preparation obtained on the base of abovementioned strain is useful in increasing of productivity of agriculture cultures and increasing resistance thereof to various diseases. Claimed preparation may be used in combination with pesticides.
Hyperthermophile strain caldothrix satsumae capable of fermentation of organic waste at high temperatures / 2291900
Strain Caldothrix satsumae YM081 is isolated from compost by fermentation of organic waste at 85°C or higher. Strain is deposited under number FERM BP-8233.
 Organic nitrogen-containing composition and fertilizer comprising thereof / 2291139
Invention proposes an organic nitrogen-containing composition comprising enzymatic mother solution prepared by culturing microorganism of genus Enterobacter that is able to produce L-glutamic acid in liquid medium. Culturing is carried out at pH value providing precipitation of L-glutamic acid under condition that L-glutamic acid is produced and accumulated with accompanying precipitation and the following separation of L-glutamic acid from medium. Microorganism can metabolize carbon source in liquid medium containing L-glutamic acid in the saturation concentration and carbon source at the definite pH value and possesses capacity to accumulate L-glutamic acid in the amount exceeding the saturating concentration of L-glutamic acid in liquid medium at this pH value. Prepared composition is used as a component of fertilizer. The claimed invention provides expanding assortment of fertilizers.
Method for compost production from organic waste / 2290390
Claimed method includes waste mixing with paunch mature as microelement source and baking or beer yeast or yeast extracts and autolysates. Mixture is moistened up to humidity of 30-50 % and composted under aerobic conditions, wherein carbon/nitrogen ratio in finished product is from 10 to 50.
Method for reprocessing of organic waste by composting with worms / 2290389
Claimed method includes preliminary composting of organic waste by introducing of paunch mature as microelement source and yeast or yeast extracts as nitrogen source. Organic waste with additives is agitated for 7-12 days to produce unripe compost. Unripe compost is mixed with waste from pulp-and paper or wood processing industry and additional nitrogen sources, molasses and earthworms. In process of composting with worms enzyme activity of composting mixture is controlled.
Method for preparing biohumus enriched with phosphorolytic microorganisms / 2286983
Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Agricultural animal manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting process is carried out for 2-3 months at temperature 16-32°C under natural conditions, and after separation of worms from biohumus microorganisms of species Bacillus megaterium var. phosphaticum are added to biohumus in the concentration 1010-1012 cells per 1 kg of biohumus. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity, decreasing material consumptions and decreasing time for preparing the preparation.
Method for preparing biohumus-base bacterial fertilizer / 2286982
Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Agricultural animal manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting process is carried out for 2-3 months at temperature 16-32°C under natural conditions, and after separation of worms from biohumus on vibrating screen with pores size 0.5-1.0 cm then biohumus is dried up to moisture 50-60%, packaged into polypropylene packets, molasses is added in the amount 1.0-3.0% of the biohumus mass, subjected for autoclaving at 0.8-1.2 atm for 45-75 min followed by addition of microorganisms of species Rhizobium japonicum. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity, decreasing material consumptions and decreasing time for preparing the preparation.
Method for preparing biohumus-base combined fertilizing soil / 2286981
Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Agricultural animal manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting process is carried out for 2-3 months at temperature 16-32°C under natural conditions, and after separation of worms from biohumus the strain Bacillus megaterium var. phosphaticum and the strain Agrobacterium radiobacter are added. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity, decreasing material consumptions and decreasing time for preparing the preparation.
Method for preparing microbiological fertilizer / 2286980
Invention relates, in particular, to technology for preparing biofertilizers. Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Cattle manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting process is carried out for 2-3 months at temperature 16-32°C under natural conditions, and after separation of worms from biohumus on vibrating screen with pores size 0.5-1.0 cm then biohumus is dried up to moisture 50-60%, packaged into polypropylene packets, subjected for autoclaving at 0.8-1.2 atm for 45-75 min followed by addition of microorganisms able for associative nitrogen fixation taken in the concentration 108-1010 cells per 1 g of the preparation. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity due to increasing viability of microorganisms, decreasing material consumptions in producing based on simplifying technology for preparing and decreasing time for preparing the preparation.
Method for preparing biohumus-base microbiological fertilizer / 2286979
Invention relates, in particular, to technology for preparing biological fertilizers. Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Agricultural animal manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting is carried out for 2-3 months at temperature 16-32°C under natural conditions, and after separation of worms from humus on vibrating screen with pores size 0.5-1.0 cm then biohumus is dried up to moisture 50-60%, packaged into polypropylene packets, corn extract is added in the amount 1.0-5.0% of the biohumus mass and subjected for autoclaving at 0.8-1.2 atm for 45-75 min followed by addition of the strain Bacillus megaterium var. phosphaticum 319 and kept up to the titer value 8 x 1010. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity due to increasing viability of microorganisms, decreasing material consumptions in producing based on simplifying technology in preparing and decreasing time for preparing the preparation.
Method for preparing combined fertilizing biopreparation for plants / 2286978
Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Agricultural animal manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting process is carried out for 2-3 months at temperature 16-32°C under natural conditions and after separation of worms from biohumus on vibrating screen with pores size 0.5-1.0 cm then biohumus is dried up to moisture 50-60%, packaged into polypropylene packets and subjected for autoclaving at 0.8-1.2 atm for 45-75 min followed by addition of the strain Bacillus megaterium var. phosphaticum 319 and the strain Agrobacterium radiobacter 204. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity, decreasing material consumptions and decreasing time for preparing the preparation.
Method of biological remediation of oil-polluted lands / 2290270
Invention relates to biotechnological techniques employed to restore lands polluted with crude oil and petroleum products. Method consists in that soil is treated with dry filled bacteriological preparation, wherein strains Pseudomonas fluorescens KO and Pseudomonas aeruginosa KOA-3 are used as microorganisms and brown coal waste used as filler. Once treated, soil is sewed with seeds of bean and cereal cultures mixture pretreated with plant growth regulator, in particular with bacteriological preparations based on Azotobacter chroococcum Mut-1, Bacillus megaterium KC-1.
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FIELD: biotechnology, industrial microbiology, ecology. SUBSTANCE: invention relates to a biological preparation used in treatment of soil polluted with petroleum and oil products in simultaneous recovery of its physicochemical properties and natural biocenosis. The biopreparation-petroleum destructor comprises microorganism culture and bacterial fertilizer as fermented bedding avian dung containing the following native microorganisms: Clavibacter michiganese, Bacillus amyloliquefaciens and Micrococcus varians. As a microorganism culture the strain Bacillus cereus 3K is used with a titer value 1012 cells/g of bacterial fertilizer. Invention provides enhancing petroleum-destructing activity, to simulate development of petroleum-oxidizing microflora, to increase the rate of petroleum decomposition, to provide intensification of bacterial processes recovering agrochemical properties of soil, to carry out treatment of petroleum-polluted soils in regions with short heat period. EFFECT: improved, enhanced and valuable properties of agent. 6 tbl, 7 ex
The invention relates to biotechnology, namely the biological preparation on the basis of microbial fertilizers (recycling poultry farms) and on the basis destructor microorganisms (bacteria of the genus Bacillus), and can be used for cleaning contaminated by oil and soil whilst restoring its physical-chemical properties and natural biocenosis. Known bacterial culture p.Bacillus, which are characterized by the ability to produce antibiotic substances and inhibit the growth of phytopathogenic microorganisms, see Junge, H., Krebs, S., Frier K.// Mitt.Biol. Land - und Frostwirt. Berlin-Dahlem. - 1990. No. 226. Known bacteriophage fertilizer containing humic-containing base and bacterial additive from the group of bacteria: Bacillus subtilis, Bacillus cereus, Bacillus polymyxa, see Russian Federation patent No. 2186049 in class. C 05 F 11/08, C 12 N 1/20, publ. 2002 In bacterial additive used bacteria Bacillus cereus, a strain D 5,0·106cells/g basis. Known strains of bacteria used to clean the soil and water from oil pollution, in particular Bacillus sp., see the patents of the Russian Federation No. 2083667 in class. C 12 N 1/20, In 09 With 1/10, C 02 F 3/34, publ. 1997, No. 2115629 in class. C 02 F 3/34, In 09 With 1/10, C 12 N 1/20 C 12 N 1/26, publ. 1998, No. 2182529 on CL In 09 With 1/10, C 02 F 3/34, C 12 N 1/26, publ. 2002, No. 2204597 in class. C 12 N 1/20 C 02 F 3/34, In 09 With 1/10, publ. 2003 Izvestnostnomu fertilizer Whirlpool, see Arkhipchenko I.A. Harvest create microbes.//Suburban life. 2001. No. 11. P.15. Known Bioorganic fertilizer on the basis of fermentation droppings of birds, containing the Association of microorganisms that can cause the biodegradation of oil and stimulate the growth of microorganisms, see Russian Federation patent No. 2141932, class C 05 F 11/08, publ. 1999 A method of obtaining fertilizer, comprising preparing a microbial mass and immobilization of biomass in organic media in the form of dry granulated chicken manure, see Russian Federation patent No. 2130005 in class. C 05 F 11/08, C 12 N 11/02, publ. 1999 Known composition for cleaning soil from oil spills containing meteorologie microorganisms and complex fertilizer, see Russian Federation patent No. 2112610 on CL In 09 With 1/10, C 02 F 3/34, C 12 N 1/26, publ. 1998 Known microbial fertilizer in the form of fermented litter poultry litter containing the native microorganisms R. Bacillus, see Fisinin VI, Arkhipchenko I.A., Popov EV, Solntseva IE the Use of poultry litter to obtain microbial fertilizers with multifunctional properties. Microbiol.//Reports RASHN. No. 2. P.32-34. All known biological products have low destructible ability. Known neftedstruktorov, including the culture of bacteria and microbial fertilizer in the form of fermenti is consistent bird droppings, containing the native microorganisms: Clavibacter michiganese, Bacillus amyloliquefaciens, Micrococcus varians, see Arkhipchenko I.A., Zagvozdina VK Cleaning oil-contaminated soil using biological products based on microbial fertilizers. //Ecology and industry of Russia. The special issue. 2004. P.16-18. This solution made the closest analogue of the present invention. However, it has drawbacks. Microbial fertilizer that has similar properties of mineral and organic fertilizers, but neftedestruktivnoi activity of the microorganism cells is not sufficient for apteekista soil in areas with short heat period. The basis of the present invention based on a technical solution that allows you to create an efficient, simple and cost-effective biologic - neftedstruktorov integrated steps: - high neftedestruktivnoi activity - with the positive properties of mineral and organic fertilizers, with simple and cost-effective mode of obtaining, with the preferred use for cleaning contaminated soils in areas with short heat period. According to the invention this problem is solved due to the fact that the biological product-neftedstruktorov culture of bacteria and microbial fertilizer in the form of fermented postiac the CSOs bird droppings, containing the native microorganisms: Clavibacter michiganese, Bacillus amyloliquefaciens, Micrococcus varians. As the bacteria cultures used a strain of Bacillus cereus 3 with title 1012cells/g of microbial fertilizers. The applicant has not identified the sources containing information about technical solutions, identical to the present invention, which allows to make a conclusion about its compliance with the criterion of "novelty". Due to the implementation of the distinguishing features of the invention /together with the characteristics specified in the restrictive part of the formula/ achieved an important new properties object. In the proposed technical solution sharing microbial fertilizer OMP containing microorganisms - Alcantara, and enriched with microorganisms - oil degrading (bacteria strain Bacillus cereus 3), allows to obtain an effective biological product - neftedstruktorov high neftedestruktivnoi activity. The applicant is not aware of any publication that would contain information about the impact of the distinctive features of invention technical result achieved in this regard, according to the applicant, it is possible to draw a conclusion on the conformity of the proposed technical solution the criterion of "inventive step". The invention consists in the following. Biologic-neftedstruktorov includes microbial easy is giving Omug and culture of the bacterial strain Bacillus cereus 3, immobilized him. Microbial fertilizer Aug made in the form of fermented litter poultry litter and contains native microorganisms sustainable Association: Clavibacter michiganese, Bacillus amyloliquefaciens, Micrococcus varians. Microbial fertilizer OMP get in the aerobic fermentation of waste poultry farms (bedding bird droppings). In fermented mass is added mineral fertilizers (NPK) with a mass of about 2% by weight of the processed material. The fermentation is carried out by aerobic thermophilic bacteria. Activation of their activity is ensured by optimizing the moisture content of the initial mixture (40%-60%), maintaining a high porosity (20%-30%). Working temperature of the mixture is maintained 55%-70% due to the heat released during the oxidation of organic matter processed material. Aerobic fermentation activates the growth of aerobic microorganisms. Culture bacteria strain Bacillus cereus St To deposited in the GSI research Institute for AGRICULTURAL MICROBIOLOGY, 2003 (date of completion 25.11.03). Culture bacteria strain Bacillus cereus isolated from 3 To technogenic soil contaminated with diesel fuel (Saint-Petersburg). From technogenic soils on the territory of the Kirov plant in the CHP (Saint-Petersburg) samples were taken. The selection was performed by seeding (by the method of serial dilution) on p is now a nutrient medium Pridham-Gottlieb, in which the only source of carbon used crude oil. The invention is illustrated by examples. EXAMPLE 1. Bioorganic media: - microbial fertilizer OMP (nearest equivalent) in the form of fermented litter poultry litter contains native microorganisms: Clavibacter michiganese, Bacillus amyloliquefaciens, Micrococcus varians, - organic fertilizer Poudret in the form of air-crude mass obtained by drying poultry manure (humidity 75%) in the infrared rays at a temperature of between 80 and 96 degrees, with an average composition dry matter (% wt.): protein nitrogen - 3,9; in the form of uric acid and 2.9, NH - 0,36-1,2; S - 34, P - 1,1; crude protein - 26-38; fiber - 123-14; carbohydrates - 35-43; nitrogen-free extractives - 30-37. Physical and chemical characteristics of fertilizers is presented in table 1. From table 1 it is evident that organic fertilizer Poudret not conducive to the utilization of oil, although it has adsorption capacity is almost 2 times lower than that of microbial fertilizer Omug. EXAMPLE 2. Bioorganic media: - microbial fertilizer Omug in the form of fermented litter poultry litter contains native microorganisms: Clavibacter michiganese, Bacillus amyloliquefaciens, Mierococcus varians, - organic fertilizer Poudret representing the air-crude mass obtained by drying poultry manure (humidity is using 75%) in the infrared rays at a temperature of between 80 and 96 degrees, with an average composition dry matter (% wt.): protein nitrogen - 3,9; in the form of uric acid and 2.9, NH - 0,36-1,2; S - 34, P - 1,1; crude protein - 26-38; fiber - 123-14; carbohydrates - 35-43; nitrogen-free extractives - 30-37. The number of trophic groups of bacteria in the media is determined by the method of Koch: sowing media on a nutrient medium Pridham-Gottlieb. As the sole source of carbon used oil - 1%. Mineral environment Pridham-Gottlieb, g/l: (NH4)2SO4- 2,64;2HPO4- 5,65; KN2PO4- 2,38; MgSO4·7H2O - 1,0; CuSO4- 0,064; FeSO4- 0,01; MnCl2- 0,079; ZnSO4- 0,015. Bacteria-ammonification account on dry nutrient agar (SPA), originarily - on environment Ashby. The results are presented in table 2. From table 2 it is evident that microbial fertilizer Pool contains microorganisms, utilizing crude oil (titer of balkantrib 106cells/g). EXAMPLE 3. Of oil-contaminated soils (40 years of pollution diesel fuel) isolated bacterium Bacillus cereus 3 pcs. K., able to grow with crude oil as the sole carbon source. Bacteria cultures equivalents: production culture of Bacillus subtilis pieces 2843, production culture of Rhodococcus erythropolis pieces 1231. Analyze neftedestruktivnoi activity of soils is the R bacteria using fluorescence spectroscopy. The results of the analysis are shown in table 3. From table 3 it can be seen that the greatest degree of degradation of oil in bacteria strain Bacillus cereus 3 K. Note: Control - nutrient medium Pridham-Gottlieb with 1% of oil. From table 3 and table 1 shows that the degree of degradation of oil in bacteria strain Bacillus cereus 3 To almost twice (1,93) higher than that of microbial fertilizer OMP (nearest equivalent). EXAMPLE 4. Bacteria strain Bacillus cereus 3 To build on the solid dry nutrient agar for 3 days. Create an aqueous suspension of bacteria strain Bacillus cereus 3 with title 1013cells/g At the surface to 1 m spread : 1 kg of air-dried microbial fertilizer Aug, razbryzgivayut 100 ml suspension of bacteria strain Bacillus cereus 3 with title 1013cells/g and receive a biological product containing bacteria strain Bacillus cereus 3 with title 1012cells/g of microbial fertilizer Omug. EXAMPLE 5. Bookmark field experiment conducted at the experimental oil-contaminated site (Usinsk) area of 2000 m2, which is made of mineral fertilizer (N, P, K) and the biological product-neftedstruktorov based microbial fertilizer OMP (100 g/m2). Samples taken over a month or two after laying of the experiment. The hydrocarbon content of the samples determined by the gravimetric method. Extraction of hydrocarbons carried out with hexane. Fractionation of n is PTI produced after pyrolysis of solids, formed after evaporation of the hexane from the hood at t=480°C. Formed after pyrolysis gas phase to analyze the chromatograph LHM-80, model No. 5. Dynamics of the concentration of hydrocarbons in oil-contaminated soil, depending on the make of the biological product-neftedstruktorov based microbial fertilizer Omug presented in table 4. Table 4 shows that the control oil fell by 6%-12%, and in oil-contaminated soil amended biological product-neftedstruktorov based microbial fertilizer Omug 32%-50%. Note: Control - oil-contaminated soil. EXAMPLE 6. Bookmark field experiment conducted at the experimental oil-contaminated site (Usinsk) area of 2000 m2, which is made of mineral fertilizer (N, P, K) and the biological product-neftedstruktorov based microbial fertilizer OMP (100 g/m2). Samples taken one month after the laying of the experiment. The number of trophic groups of bacteria in oil-contaminated soils and oil-contaminated soil amended biological product-neftedstruktorov on the basis of microbial fertilizers Whirlpool determined by the method of Koch: the planting soil nutrient medium Pridham-Gottlieb. As the sole source of carbon used oil - 1%. Mineral environment Pridham-Gottlieb, g/l: (NH4)2SO4- 2,64;2HPO - 5,65; KN2PO4- 2,38; MgSO4·7H2O - 1,0; CuSO4- 0,064; FeSO4- 0,01; MnCl2- 0,079; ZnSO4- 0,015. Bacteria-ammonification account on dry nutrient agar (SPA), originarily - on environment Ashby. Population dynamics of bacteria in oil-contaminated soil, depending on the make of the biological product-neftedstruktorov on the basis of microbial fertilizers Whirlpool is shown in table 5. From table 5 it is seen that in oil-contaminated soil amended biological product-neftedstruktorov based microbial fertilizer Omug the number of bacteria increased in average by 35% compared to control. Note: Control - oil-contaminated soil. EXAMPLE 7. Bookmark field experiment conducted at the experimental oil-contaminated site (Usinsk) area of 2000 m2, which is made of mineral fertilizer (N, P, K) and the biological product-neftedstruktorov based microbial fertilizer OMP (100 g/m2). Samples taken one month after the laying of the experiment. The biological activity of oil-contaminated soils and oil-contaminated soil amended biological product-neftedstruktorov based microbial fertilizer Omug judged by the intensity of their CO2. CO2determined by gas chromatography. A portion of the soil mass 20 g (calculated on bsolute dry weight) are placed in a flask with a volume of 120 ml, cover with a cotton stopper and incubated in a humid chamber at a temperature of 27°C. the Intensity of selection WITH2determined after 3 days. To this flask, "washed" with compressed air for 3 minutes and hermetically closed by plastic caps with elastic rubber gasket. Incubated for 24 hours in a thermostat at a temperature of 27°C. After this medical syringe take samples of gas with a volume of 0.5 ml and injected them into the sink gas chromatograph Chrom-4, first make its calibration. For chromatography using sorption column stainless steel length of 120 cm with an inner diameter of 2 mm, filled with sorbent Polisorb-1. As the gas used is helium, which is passed at a speed of 20 mm/min. Temperature thermostat detector 100°C. chamber Temperature and columns 20-22°C. the output of the CO2occurs at the 48th second, the chromatograph is a clear peak securing the release of CO2. The intensity of selection CO2in oil-contaminated soil, depending on the make of the biological product-neftedstruktorov based microbial fertilizer Omug presented in table 6. From table 6 it is seen that in oil-contaminated soil amended Biopreparat-neftedstruktorov on the basis of microbial fertilizers Whirlpool CO2outstanding 5-fold compared to control. Note the Chani: Control - oil-contaminated soil. These examples suggest that the biological product-neftedstruktorov obtained by immobilization of bacteria strain Bacillus cereus 3 on microbial fertilizer Aug, is a promising biological drug is able to enhance microbial processes in soil, and has neftedstruktorov activity two times higher than the nearest equivalent. The proposed biological product-neftedstruktorov based microbial fertilizer Omug used for treatment of soils contaminated with oil after an oil spill in the vicinity Gusinsky located in Usinsk-Kolvinskoye spruce-forest-tundra region in the zone of forest-tundra and crainiceanu taiga Caves-Ural territory, observing the dynamics of the processes in oil-contaminated soil and evaluation of the enhanced recovery of soils with the introduction of the biological product to confirm its compliance with the criterion of "industrial applicability". The effective use of the biological product-neftedstruktorov for cleaning contaminated soils allows you to: to improve neftedstruktorov activity, - to encourage the development of oil-oxidizing microflora, - to increase the rate of decomposition of oil, - to support the intensification of microbial processes, restoring agrochemical properties of soil, -to combine the positive properties of mineral and organic fertilizers, - to clean oil-contaminated soils in areas with short heat period.
Biologic-neftedstruktorov, including the culture of bacteria and microbial fertilizer carrier in the form of fermented litter poultry litter containing the native microorganisms: Clavibacter michiganese, Bacillus amyloliquefaciens, Micrococcus varians, characterized in that as the bacteria cultures used a strain of Bacillus cereus 3 with title 1012to etoc/g microbial fertilizers.
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