Method for treatment of alfalfa seeds |
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IPC classes for russian patent Method for treatment of alfalfa seeds (RU 2299188):
Bacillus subtilis 1719 bacterium strain as producer of antagonistically active biomass in relates to pathogenic microorganisms, as well as proteolytic, amylolytic, and lipolytic enzymes / 2298032
Invention relates to Bacillus subtilis 1719 bacterium strain isolated under natural conditions in microbiological laboratory.
Actinomyces fradiae strain for reprocessing of organic waste from animal and poultry breeding / 2298031
Disclosed is Actinomyces fradiae-96 strain isolated from soil sampled form organic poultry breeding waste.
Method for production of androsta-1,4-diene-3,17-dione from plant and animal derived sterols / 2297455
Invention relates to microbial production of androsta-1,4-diene-3,17-dione (ADD). Claimed method is based on ability of specific Mycobacterium neoaurum bacteria strain to selectively eliminate side chain of abovementioned sterols and to accumulate ADD in presence of transformation product sorbent. As sorbents synthetic polymer resins obtained by copolymerization of divinylbenzene and ethylstyrene are used. Sorbents are added in broth in end of lag-phase. ADD is formed with yield of 66-76 % depending on side chain structure of transformed substrates.
 Polypeptides derived from tryptophanyl-trna-synthetase and uses thereof in controlling of vascularisation / 2297425
Invention relates to water soluble polypeptides (SEQ ID NO.12) and (SEQ ID NO.7), derived from full-length tryptophanyl-tRNA-synthetase and having angiostatic activity in relation to eye neovasculatisation. Also disclosed are polynucleotides, encoding truncated polypeptide forms (SEQ ID NO.12) and (SEQ ID NO.7), and E.coli cell, expressing abovementioned polypeptides. Said polypeptides are useful in injecting angiostatic composition and kit for inhibiting of eye neovasculatisation.
Bifidum bacterium strain consortium to produce sour-milk, fermented and non-fermented foodstuffs, ferments, bioactive supplements, bacterium preparations, and cosmetic agents / 2296797
Claimed consortium is produced by combination of four bifidum bacterium strains, namely B.bifidum 79-37, B.longum, B.breve 79-88, B.infantis 79-43. Said consortium actively propagates in nutritional media with biomass accumulation in short culturing time; ferments milk with clot production having good taste and organoleptic characteristics; and has specific activity. Consortium is capable of human intestinal microbial population normalizing in short time and is useful in production of healthy products with good organoleptic characteristics.
Method for production of peat-grains compost / 2296732
Claimed method includes blending of beer grains with peat in ratio of 1:1 or 1:2 followed bioactivator introducing and aerobic composting at 20-60°C and humidity of 65-70 % for 2-3 months. Compost ferment prepared by culturing of microorganism consortium, isolated from humified humous forest soil layers and containing fungi Aspergillus niger, Aspergillus oryzae, Aspergillus sydowii, Cephalosporium, Glyocladium Cda., Trichoderma sp., actinomycetes Streptomyces griseus, Streptomyces termoviolaceus, Streptomyces globisporus, Streptomyces rubber, Streptomyces viridosporus and bacteria Bacillus cereus, Bacillus mycoides, Bacillus sublilis, in amount of 15 % or more based on composing mixture mass on beer grains is used as bioactivator.
Consortium of bifidobacterium strains to prepare lactic fermented and non-fermented food products, biologically active additives, leavens, bacterial preparations, hygienic agents, and cosmetics / 2296158
Invention relates to food processing, cosmetic, biotechnological, and medicinal industries. Consortium is prepared by way of combining eight bifidobacterium strains: B. bifidum 79-37, LVA-3, B. Longum V79M, Ya-3, Ya-4, B. breve 79-88, B. Unfantis 79-73, B. adolescentis GO-13. Proposed eight-strain consortium is grown on nutrient media to accumulate production mass within short culturing terms, ferments milk to form clot having good flavor and organoleptic properties, and possesses specific activity. Eight-strain consortium showing high specific activity, is capable of rapidly ecizing in human bowels and promotes normalization of gastric microflora within short periods of time. Consortium may also be a source of therapeutical and prophylactic food products having good organoleptic properties.
Consortium of bifidobacterium strains to prepare lactic fermented and non-fermented food products, leavens, biologically active additives, bacterial preparations, hygienic agents, and cosmetics / 2296157
Invention relates to food processing, cosmetic, biotechnological, and medicinal industries. Consortium is prepared by way of combining four bifidobacterium strains: Bifidobacterium bifidum 79-37, LBA-3, B. longum B379M, and Ya-4. Consortium is actively grown on nutrient media to accumulate production mass within short culturing terms, ferments milk to form clot having good flavor and organoleptic properties, and possesses specific activity. Four-strain consortium, showing high specific activity, is capable of rapidly ecizing in human bowels and promotes normalization of gastric microflora within short periods of time. Consortium may also be a source of therapeutical and prophylactic food products having good organoleptic properties.
 Strain bifidobacterium lactic 672 used to prepare lactic therapeutic and prophylactic products, fermented and non-fermented food products, biologically active additives, probiotic, and cosmetics / 2296156
Invention is intended for use in, food processing, cosmetics, biotechnological, medicinal industries, and veterinary in order to strengthen health, in particular when preparing lactic fermented and non-fermented food products, cosmetics, biologically active additives, and probiotic. Strain Bifidobacterium lactis 672 BKPM As-1677 is isolated from contents of healthy baby bowels. Invention enhances biological activity of the strain and its resistance to environmental conditions, accelerates growth on artificial nutrient media and acid-forming and antagonistic activity with regard to pathogenic and conditionally pathogenic microflora. Preparation of strains possessing above properties promotes extension of assortment of probiotics used to maintain health, in particular to normalize microflora gastroenteric and urogenital tracts, human and animal cutaneous coverings and mucous membranes.
 Bacterium strain borrelia garinii bgvir-1 for preparation of drugs for diagnosis and prophylaxis of ixodic mite-born borreliosis and for evaluation of protective and specific activity of prophylaxis and therapeutic agents / 2295564
Bacterium strain Borrelia garinii BgVir-1 synthesizes all basic immunogenic proteins of borrelia from group B. burgdorferi s.L and is useful in production of diagnostic preparations and vaccine.
Actinomyces fradiae strain for reprocessing of organic waste from animal and poultry breeding / 2298031
Disclosed is Actinomyces fradiae-96 strain isolated from soil sampled form organic poultry breeding waste.
Method for production of peat-grains compost / 2296732
Claimed method includes blending of beer grains with peat in ratio of 1:1 or 1:2 followed bioactivator introducing and aerobic composting at 20-60°C and humidity of 65-70 % for 2-3 months. Compost ferment prepared by culturing of microorganism consortium, isolated from humified humous forest soil layers and containing fungi Aspergillus niger, Aspergillus oryzae, Aspergillus sydowii, Cephalosporium, Glyocladium Cda., Trichoderma sp., actinomycetes Streptomyces griseus, Streptomyces termoviolaceus, Streptomyces globisporus, Streptomyces rubber, Streptomyces viridosporus and bacteria Bacillus cereus, Bacillus mycoides, Bacillus sublilis, in amount of 15 % or more based on composing mixture mass on beer grains is used as bioactivator.
Method for production of fermented agriculture composition / 2296112
Claimed method includes microbial decomposition of plant waste in oxygen-enriched air forced circulation, wherein oxygen is obtained by Chlorella Vulgaris culturing. Carbon dioxide released in process of plant waste decomposition is collected and used in algae culturing. Method also includes manure deodorizing with calcium oxide or calcium hydroxide. Deodorized manure is mixed with mass obtained in process of plant waste decomposition. Mixture is fermented for 4-5 days and mixed with solid fraction of Chlorella Vulgaris. Said solid fraction of Chlorella Vulgaris in obtained by double freezing and defrosting of algae biomass and cell juice separation. Process is carried out in closed cycle. Obtained composition is useful both as fertilizer and feed supplement.
Biopreparation-petroleum destructor / 2292326
Invention relates to a biological preparation used in treatment of soil polluted with petroleum and oil products in simultaneous recovery of its physicochemical properties and natural biocenosis. The biopreparation-petroleum destructor comprises microorganism culture and bacterial fertilizer as fermented bedding avian dung containing the following native microorganisms: Clavibacter michiganese, Bacillus amyloliquefaciens and Micrococcus varians. As a microorganism culture the strain Bacillus cereus 3K is used with a titer value 1012 cells/g of bacterial fertilizer. Invention provides enhancing petroleum-destructing activity, to simulate development of petroleum-oxidizing microflora, to increase the rate of petroleum decomposition, to provide intensification of bacterial processes recovering agrochemical properties of soil, to carry out treatment of petroleum-polluted soils in regions with short heat period.
 Hyperthermophile strain caldothrix satsumae capable of fermentation of organic waste at high temperatures / 2291900
Strain Caldothrix satsumae YM081 is isolated from compost by fermentation of organic waste at 85°C or higher. Strain is deposited under number FERM BP-8233.
 Organic nitrogen-containing composition and fertilizer comprising thereof / 2291139
Invention proposes an organic nitrogen-containing composition comprising enzymatic mother solution prepared by culturing microorganism of genus Enterobacter that is able to produce L-glutamic acid in liquid medium. Culturing is carried out at pH value providing precipitation of L-glutamic acid under condition that L-glutamic acid is produced and accumulated with accompanying precipitation and the following separation of L-glutamic acid from medium. Microorganism can metabolize carbon source in liquid medium containing L-glutamic acid in the saturation concentration and carbon source at the definite pH value and possesses capacity to accumulate L-glutamic acid in the amount exceeding the saturating concentration of L-glutamic acid in liquid medium at this pH value. Prepared composition is used as a component of fertilizer. The claimed invention provides expanding assortment of fertilizers.
Method for compost production from organic waste / 2290390
Claimed method includes waste mixing with paunch mature as microelement source and baking or beer yeast or yeast extracts and autolysates. Mixture is moistened up to humidity of 30-50 % and composted under aerobic conditions, wherein carbon/nitrogen ratio in finished product is from 10 to 50.
Method for reprocessing of organic waste by composting with worms / 2290389
Claimed method includes preliminary composting of organic waste by introducing of paunch mature as microelement source and yeast or yeast extracts as nitrogen source. Organic waste with additives is agitated for 7-12 days to produce unripe compost. Unripe compost is mixed with waste from pulp-and paper or wood processing industry and additional nitrogen sources, molasses and earthworms. In process of composting with worms enzyme activity of composting mixture is controlled.
Method for preparing biohumus enriched with phosphorolytic microorganisms / 2286983
Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Agricultural animal manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting process is carried out for 2-3 months at temperature 16-32°C under natural conditions, and after separation of worms from biohumus microorganisms of species Bacillus megaterium var. phosphaticum are added to biohumus in the concentration 1010-1012 cells per 1 kg of biohumus. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity, decreasing material consumptions and decreasing time for preparing the preparation.
Method for preparing biohumus-base bacterial fertilizer / 2286982
Method involves vermicomposting of organic waste by using hybrid of red California worm with earthworm of the Kuban natural population taken in the amount 104 worms per m2. Agricultural animal manure neutralized preliminary to pH 7-8 is used as organic waste. Vermicomposting process is carried out for 2-3 months at temperature 16-32°C under natural conditions, and after separation of worms from biohumus on vibrating screen with pores size 0.5-1.0 cm then biohumus is dried up to moisture 50-60%, packaged into polypropylene packets, molasses is added in the amount 1.0-3.0% of the biohumus mass, subjected for autoclaving at 0.8-1.2 atm for 45-75 min followed by addition of microorganisms of species Rhizobium japonicum. Invention provides expanding assortment of microbiological fertilizers, enhancing their biological activity, decreasing material consumptions and decreasing time for preparing the preparation.
Biological fertilizer / 2249584
Biological fertilizer contains (%): growth agents (e.g., aqueous extract from Kalanchoe leaves) 3; inhibitor of soil pest growth and fungal and bacterial diseases (e.g., soil-entomopatogenic associative nematodes and waste products thereof, as well as living nematode-symbiotic bacteria) 0.001 and 0.0001, respectively; feed source (e.g., sodium humate) 0.1; and balance: water. Fertilizer of present invention finds plants with feedstuff, physiologically active substances and reduces helpful symbiotic microflora of rhizosphere.
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FIELD: soil microbiology, agriculture. SUBSTANCE: invention relates to methods used in treatment of alfalfa seeds. Method involves presowing treatment of alfalfa seeds with biopreparation based on the strain Sinorhizobium meliloti Yakutsky №1 GNU VNIIChM 1775 taken in the amount 1 billion of cells per 1 seeds of alfalfa. Method provides enhancing productivity of alfalfa and improving fertility of soils. EFFECT: improved method of treatment of seeds. 5 tbl, 5 ex
The invention relates to the field of agriculture: crop production, forage production, cultivation, agriculture, soil science, and soil Microbiology. A method of processing seeds of alfalfa (Novikov A.G. Knyazeva V.L., Onishenko N.G. all-Union collection Rhizobium is the main gene pool of symbiotic nitrogen fixing bacteria. Proceedings of the practical approach, 1989, vol. 59, s-22) using biomass strains Rizobium meliloti). The technical result of the invention is the increased productivity of alfalfa and improvement of soil fertility. The technical result is achieved in the following way: from a pure culture is made of liquid bacterial preparation on the basis of strain Sinothizobium meliloti Yakut No. 1 wildebeest ARRIAM 1775 nodule bacteria (KB) of alfalfa in the amount of 1 billion cells per 1 seed alfalfa. The strain of nodule bacteria Yakut No. 1 wildebeest ARRIAM 1775 characterized by the following features. Morphological features: The shape of cells: rod-shaped, straight or slightly curved motile, have polar flagella - peritricha from 1 to 3. Sticks - 0,5-0,9×1.2 to 3.0 mm. Gram stain - gram. Mobility - mobile due to flagella. The presence of pigment - forming. The sporulation - does not form spores. The cultivation conditions (medium, temperature, the ratio of oxygen and other) belong to ustroystvom. The fast-growing root nodule bacteria growth in isolated small colonies apparent after 3-5 days of incubation pulses environment. The colonies are round, convex, translucent, raised, mucous, usually 2-4 mm Optimum temperature 25-30°C, the optimum pH range 6-8. Legumes medium: broth peas - 1 l Sucrose (sugar) - a 20 g Agar-agar - 20 g Sterilization at 1 ATM 20 minutes Relation to oxygen aerobic: the respiratory metabolism of the type using oxygen as the final electron acceptor. The sources of carbon using the following substrates: D-arabinose, cellobiose, fructose, D-galactose, glucose, lactose, mannitol. The sources of nitrogen ammonium chloride and nitrates. With the growth of culture with milk litmus paper strongly acidified environment, giving it a pink color. Other signs of strains need Pantothenate and nicotinic acid requirements of vitamins vary. Casein and agar are not hydrolized. 3-ketogluconic do not form. Oxidase, catalase positive. Symbiotic nitrogen fixing bacteria. The characteristic feature of these organisms is the ability to penetrate the root hairs of alfalfa, sweet clover and cause the formation of root nodules in which the bacteria are present as intracellular symbionts. In club root is Kah bacteria are present in the form of pleomorphic form bacteriodes, usually effecting the fixation of atmospheric nitrogen by converting it into a linked form (ammonia), available for use by the host plant. From a pure culture prigotovlen liquid AMF inoculum. Pure culture is periodically transferred culture. The strain can be obtained in the GSI ARRIAM in St. Petersburg on peat-based and in the laboratory of plant breeding and seed production of perennial grasses Yakutsk agricultural research Institute WITH RAAS in the form of liquid inoculum. Prigotovlen liquid medication into two stages: 1. Getting uterine cultures. 2. Obtain working solutions. Below are examples of implementation of the method. Example 1. The use of bacterial drug increases the yield of green mass of alfalfa in the field.
It is to be seen, from table 1 yields of green mass of alfalfa you can get up to 20% higher seed treatment on the basis of strain S.M. Yakut No. 1 wildebeest ARRIAM 1775. In comparison with production by this strain index strain S.M. Yakut No. 1 wildebeest ARRIAM 1775 exceeds 7% higher. Example 2. As can be seen from table No. 2, processing of alfalfa seed S.M. Yakut No. 1 wildebeest ARRIAM 1775 the biological yield of seeds, increased in 2 times than the control.
Example 3. The influence of strains of Sinorhizobium meliloti Yakut No. 1 wildebeest ARRIAM 1775 nodule bacteria on alfalfa agrochemical composition of the soil permafrost-taiga-fawn asolutely soil. Under grass alfalfa increased humus content of 0.5 and 0.8%as mowing aboveground mass entails more intensive renovation of the root system and, accordingly, the death of root hairs and their accumulation in the organic part of the soil. Unexplained the fact that there are beings who spent the consumption of nutrients from the root zone soil layer, the content of available phosphorus and potassium were not decreased with age of the stand. In General, inoculation of alfalfa has a positive effect in the preservation and increase of humus in the soil.
Example 4. The use of the bacterial preparation positively affects the formation of the symbiotic system of alfalfa. As can be seen from table No. 4, the number of nodules on the roots of alfalfa compared with control 2.9 million units/ha, dry weight of nodules 3.1 kg/ha, dry weight of roots of 14.4 t/ha
Example 5. Also positively influence the Yakut strains KB on the biochemical composition of alfalfa. Our observations found that the content of crude protein increased by 2.6%, crude fiber decreased by 0.7%, crude fat 0.5%, crude ash decreased by 0.1%, carotene increased by 0.6 mg/kg than in the control (table No. 5).
Apply liquid AMF inoculum from the strain of Sinorhizobium meliloti Yakut No. 1 wildebeest ARRIAM 1775 as the processing of alfalfa seed. Strain Sinorhizobium meliloti Yakut No. 1 wildebeest ARRIAM 1775 obtained from nodules of alfalfa varieties Yakut yellow, zoned in Yakutia 1999 The application of biological bacterial preparation on the basis of strain Sinorhizobium meliloti Yakut No. 1 wildebeest ARRIAM 1775 yield of green mass of alfalfa may increase up to 20%, seeds in 2 times than in comparison with no treatment. Thus, the use of strain Sinorhizobium meliloti Yakut No. 1 wildebeest ARRIAM 1775 nodule bacteria is an effective way of increasing the productivity of alfalfa and increases the humus content from 0.5 to 0.8%. The method of processing of alfalfa seed, including preplant seed Lucerne is a biological product, characterized in that the use of a biological product on the basis of strain Sinorhizobium meliloti Yakut No. 1 wildebeest ARRIAM 1775, in the amount of 1 billion cells per 1 seed alfalfa.
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