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RussianPatents.com
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For preparing nutrient medium the whole cereal and/or legume grains are used, which are moistened to 15-20%, incubated for 15-30 minutes and subjected to thermal influence in the infrared field for 40-80 sec, maintaining the temperature at the surface of grains within 115-130°C. Hot grain is incubated for 15-30 min, cooled to a temperature of 24-27°C, milled to a particle size of 70-90 microns and the suspension is prepared of the milled grain in water. Enzymatic hydrolysis is carried out of the milled raw material by the exposure of preparations of thermostable alpha-amylase and beta-glucanase for 55-60 min at pH 5.5-6.0 and temperature 65-95°C, and then subjecting the cellulase and cellobiase preparations at 55-60°C to achieve the concentration of reducing substances of 7.5-9.5%. In the fermented suspension mineral salt solution is introduced, heated to 78-80°C, incubated for 10-12 minutes and cooled to 32-33°C. In the resulting nutrient medium the yeast Saccharomyces cerevisiae culture is introduced and the yeast growing is carried out under conditions of intensive aeration to reduce the concentration of reducing substances of 0.3% and less. |
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Method of growing colonies of microbial cells and device for its realisation Group of inventions relates to biotechnology. Claimed is a method of growing colonies of microbial cells on a surface of a porous plate. The method includes supply of a nutrient solution from bottom to top through the porous plate into zones of growth of colonies of the microbial cells on its upper surface, supply of a suspension of the microbial cells onto the upper surface of the porous plate, creation of controlled conditions for the colony growth, performing observation of the colony growth, separation of the grown colonies of the microbial cells from the zones of growth and their transfer into external means of identification. The nutrient solution is supplied into the zones of growth of the colonies of the microbial cells by creation of a pressure difference between the hole input and output. Holes are made in the plate from an anode aluminium oxide orthogonally to its large plane and are topologically coded. The said zones of growth are formed in them in the form of porous membranes. The porous membranes are located at the same level as the upper surface of the plate or with formation of a hollow and do not pass the microbial cells. After supply of the nutritional solution, the suspension of the microbial cells of a specified concentration is supplied onto the upper surface of the plate until their homogenous distribution is achieved. Between the zones of growth on the surface of the plate a film, preventing attachment of the microbial cells, is formed. Separation of the grown microcolonies from the zones of growth is performed by hydroblow. A hydroblow is directed from the side of the input of cylindrical holes of the plate and spreads along them and farther through the pores of the porous membranes with force, which does not destroy the microcolonies but is sufficient for their separation from the growth zones. Also claimed is a device for growing the colonies of the microbial cells by the claimed method. |
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Inventions relate to the field of immunology. Claimed are a single-chain antibody, specific to a carcinoembryonic antigen, a chimeric mononuclear T-cell receptor, a vector, a host cell and a method of diagnostics or treatment of diseases, characterised by the presence of antigens, capable of binding with the chimeric receptor. Described is a genetic construction, coding chimeric monomolecular T-cell receptors, in which an effector fragment of the T-cell receptor is combined with an antigen-recognising part, which represents variable fragments of two different antibodies to the carcinoembryonic antigen (CEA). |
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Differentiation of human embrionic stem cells in line of pancreatic endocrine cells Invention relates to the field of biotechnology. A method includes stages of cultivating cells, expressing markers, characteristic of a line of pancreatic endocrine cells, in a medium, which contains a sufficient amount of a cyclin-dependent kinase inhibitor to induce increase of MAFA expression. As the cyclin-dependent kinase inhibitor used is ethyl-(6-hydroxy-4-phenylbenzo[4,5]furo[2,3-b])pyridine-carboxylate. The invention can be used in medicine. |
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Method of human protein cxcl1 immunoassay Invention relates to biochemistry. A method of immunoassay of human protein CXCL1 is described. Human CXCL1 or its fragment is measured in a sample with application of two or more types of monoclonal antibodies to human CXCL1 or their fragments. Each of two or more types of the monoclonal antibodies to human CXCL1 or their fragments specifically identifies any of regions of a sequence of amino acid sequences, represented in SEQ ID NO:1-3, which represent partial sequences of an amino acid sequence, constituting human protein CXCL1. Two or more types of the monoclonal antibodies to human CXCL1 or their fragments specifically identify regions of the sequence, different from each other. Claimed are the monoclonal antibodies or their fragments, each of which specifically identifies any region of the amino acid sequence, represented in SEQ ID NO:1-3, and has a new amino acid sequence. |
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Invention is a method of determining in an athlete of state of fatigue and a state of "overtraining" on increased gene expression of tryptophanyl-total RNA synthetase (TRSase). The method comprises taking a control sample from the athlete prior to intense training and the test sample after an intense training. As the sample the sample of blood or scraping or flushing with the oral cavity is used. The obtained cells are selected and washed. Total RNA is isolated from the cells. Reverse transcription is carried out and then amplifying of the cDNA obtained. The gene expression in TRSase is assessed in test and the control samples. Fatigue state and the state of "overtraining" is determined in case of a significant increase in gene expression level of TRSase in the test sample compared with a control sample, namely more than 1.45 times. |
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Strain of bacteria Exguobacterium mexicanum RNCIM V-11011 is grown, and the suspension is made from it, which is applied in the cryomorphic soil and water environment. It is exposed under the specified parameters from 7 to 60 days and the quantitative content of oil and petroleum products in the test soil and water environment is determined. |
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Method of purifying aqueous solution containing nickel salt from nickel ions Invention relates to biotechnology. Disclosed is a method of purifying an aqueous solution containing a nickel salt from nickel ions. The method involves contacting an aqueous solution containing 20 mg Ni2+/l with 0.2 g suspension of a homogenised culture per 1 l solution. The suspension has the following composition of dominant types of cyanobacteria: Phormidium ambiguum (Jom.), Phormidium boryanum (Kutz.), Leptolyngbya foveolarum (Rabenhor stex Gom), Plectonema boryanum (Gom.f.boryanum). Contacting is carried out for 1-3 hours. |
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Invention relates to the field of biotechnology and genetic engineering and can be used in veterinary for creation of vaccines regulating the sexual function in animals. A gene of a hybrid protein GHbc is obtained by the PCR method and is inserted into a polylinker region of a plasmid vector pUC9. |
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Strain Moraxella bovoculi has antigenic, virulent properties and immunogenic activity. It is deposited under the number "SH-Ch6 N-DEP" in the All-Russian State Collection of strains of microorganisms used in veterinary medicine and animal husbandry FSBI "Russian national centre of quality and standardisation of drugs for animals and feed". The strain can be used in manufacture of diagnostic preparations and vaccines against infectious keratoconjunctivitis of cattle. |
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Beverage is produced by way of dihydroquercitin solution (in an amount of 0.2-1.6 kg per 1000 dal of the beverage) introduction into water or a water-and-alcohol solution. Then, for dihydroquercitin solution stabilisation, one adds an additional antioxidant represented by molecular hydrogen in an amount of 0.2-0.8 kg per 1000 dal of the beverage. |
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Invention refers to biotechnology, particularly to genetically engineered production of human proteins, and may be used for preparing human epidermal growth factor (hEGF) in bacterial cells in the form of glutathione-3-transferase fusion protein. What is constructed is the recombinant DNA coding GST-hEGF fusion protein which consists of an amino acid sequence of glutathione-S-transferase and an amino acid sequence of human epidermal growth factor divided by a cleavage site by enterokinase, and characterised by the nucleotide sequence SEQ ID NO:1. The KpnI/XhoI fragment of the vector pET41 and the above recombinant DNA are used to create the recombinant plasmid pAS007 for expression of GST-hEGF fusion protein in E.coli cells. |
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Liquefied biomass, method of its obtaining, its application and method of its fermentation Invention relates to biotechnology. Claimed is a method of obtaining a liquefied biomass, including obtaining the biomass material from sugar beet and/or sugar cane, liquefying the said biomass with enzyme mixture. The said enzyme mixture is used in an amount of at least 0.025% of the biomass. The enzyme mixture includes cellobiohydrolase, beta-glucosidase and polygalacturonase and additionally one or several enzymes with hemicellulase activity, selected from arabinase, xylanase, pectinmethylesterase, ramnogalacturonase and 1,3-/1,6-beta-D-glucanase. Resulting product contains less than 2 wt % of a residual insoluble dry substance. |
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Method of generating antigen-specific cytotoxic cells with antitumour activity in breast cancer Method comprises isolation of mononuclear cells (MNC) from peripheral blood of a patient, separation of cells to adherent and non-adherent fractions, addition of the adherent fraction to the MNC of growth factors, loading of the dendritic cell with antigens of tumour lysate in vitro, the stimulation of maturation of dendritic cells for the next day. At that, the obtained immature DCs are added to lysate-autologous tumour cells at a dose of 100 mcg/ml, and after 48 hours within the subsequent 24 hours the rf-tumour necrosis factor-alpha is applied at a dose of 25 ng/ml. Then, the co-culture is carried out of mature dendritic cells activated with lysate and the non-adherent fraction of MNC at a ratio of 1:10 in the presence of recombinant human interleukin-12 at a dose of 10 ng/ml and the recombinant human interleukin-18 at a dose of 100 ng/ml. |
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Microbiological method of obtaining 1,2-propanediole Propanediol-oxydoreductase coding gene is introduced into an E.coli cell, which makes it possible to produce high levels of 1,2-propanediol, in fact, without 1,3-propanediol, with growing on glycerol as the only carbon source. Genes of glycerol dehydrogenase (gldA), dihydroxyacetone kinase (dhaK) and/or methylglyoxal synthase (mgsA) can be additionally inserted to express the said enzymes together with propanediol-oxydoreductase (fucO), as well as a gene of glycerol dehydratase or aldo-keto reductase in order to express the said glycerol dehydratase or aldo-keto reductase together with propanediol-oxydoreductase. The E.coli cell, transformed by the propanediol-oxydoreductase coding gene, can be defective in arabinose, methylglyoxal and/or dihydroxyacetonephosphate metabolism. |
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Group of inventions relates to biotechnology. The strain Bifidobacterium lactis CNCM I-3446 is applicable in preparing a probiotic composition for stimulating development of initial bifidogenic intestinal microbiota in the children delivered by caesarean section. The strain may be used in preparing the probiotic composition for reducing a risk of further development of allergy, as well as for preventing or treating diarrhoea in these children. |
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Method for dental restoration and method for making restoration material Group of inventions refers to medicine, namely to dentistry, and may be used for making a restoration material used for partial dental restoration in the oral cavity. That is ensured by placing the first cell mass formed by mesenchymal or epithelial cells/cell and a second cell mass formed by other mesenchymal or epithelial cells/cell on a carrier. One of the mesenchymal or epithelial cells is made from a dental germ, and the above cell masses are placed in tight contact to each other, but not mixed. The above cell masses are grown to form the whole restored tooth or its germ. That is followed by localising the whole restored tooth or its germ grown that enables implanting the whole restored tooth or is germ within the lost tooth so that a dental crown is directed inside the oral cavity with the dental germ or the tooth being used as the restoration material for preparing an equivalent of the lost tooth within the lost tooth area. |
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Bacterial strain Rhodococcus aetherivorans of Russian classification of microorganisms BKM Ac-2610D is proposed. The bacterial strain is different in growth on the common organic-mineral medium at high nitrile hydrase activity, reaching 332 U/mg at 20°C or 521 U/mg at 25°C. The nitrile hydrase of the bacterial strain Rhodococcus aetherivorans of Russian classification of microorganisms BKM Ac-2610D is thermostable. Also the method of culturing this strain is provided. The cells of the strain are inoculated on the slant meat-and-peptone agar and grown for 24-48 hours. Then the biomass is washed with sterile saline solution with pH 7.0-7.4. The resulting suspension is inoculated to the first container with the nutrient medium. The process is carried out for 24-48 hours at 28-30°C, with the circumferential stirring at a speed of 140-160 rev/min until obtaining the optical density of the suspension of 2-16 units at 540 nm and the magnitude of the optical layer of 5 mm. The resulting suspension is inoculated to the second container, which volume is 10-100 times greater than that of the first container. The value of optical density in the second container is adjusted to 0.1-0.3. The strain is cultured for 48-120 hours at 26-31°C, the aeration of 0.5-1.0 the air volume/medium volume per minute until the value of optical density reaches 36-40 and pH 7.5-7.8. The resulting biomass is separated. Also the method of production of acrylamide is provided. Hydration of acrylonitrile is carried out with the acrylonitrile concentration not exceeding 0.5%. The hydration is carried out using the biomass of bacterial strain Rhodococcus aetherivorans of Russian classification of microorganisms BKM Ac-2610D based on the order of 400-500 g of dry weight of strain per 1 ton of the final product - acrylamide with the concentration of 45-49%. The inventions enable to obtain cells of Rhodococcus aetherivorans of Russian classification of microorganisms BKM Ac-2610D of 10-18 g/l with the nitrile hydrase enzyme activity of 250-332 U/mg. |
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Method comprises euthanasia of infant rabbits by decapitation, removing paws, tails, removing skins, opening the abdomen cavity at the level of the lumbar vertebrae, kidney extraction, removal of the organ capsule, shredding into pieces with the size of 1-3 mm. The donors of the primary cell culture the newborn rabbits 1-2 days old are used. The pieces of tissue are washed from residual blood with saline solution of Hanks, subjected to disaggregation in 0.25% trypsin solution by preliminary incubation of the pieces at 37°C for 30-40 minutes, the cells are pelleted by centrifugation at 1000-1500 rev/min for 10 minutes, the supernatant is drained off, and the pellet is resuspended in the growth medium consisting of medium Igla MEM, 0.5% solution of lactalbumin (1:1) with the pH 7.0-7.2 with 10% serum of adult cattle or foetuses of cows, the cell concentration is adjusted to 5-7·105 cells/cm3, then antibiotic ciprofloxacin at 100 U/ml is added. At that the sensitivity of primarily trypsinised cultures of kidney cells of newborn rabbits to respiratory-enteric viruses of cattle, parvoviruses, herpesviruses and parainfluenza-3 by determining the viral titers in lg 50/ml TDC is determined. |
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Method of breeding coccinellidae harmonia axyridis hall Invention relates to biotechnology, in particular to methods of breeding beneficial insects. The method comprises preparing the nutrient medium comprising soy flour, sucrose, milk powder, palm kernel oil, dry aphides, Wesson salt, dry brewer's yeast, tocopherol, ascorbic acid, agar-agar, vitamins B1, B6, B12, metaben, inositol, and distilled water at a predetermined ratio and growing coccinellidae Harmonia axyridis Hall. at a temperature of 23-26°C in the prepared medium, applied to pieces of sterile polyethylene film. At that the change of feed is carried out daily. |
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Method and device for cell sorting Invention relates to biotechnology and represents a device and a system for identification and selective change of a required subpopulation of cells in a population with cell samples. The device and system include a path of liquid movement. The device and system include application of an objective, an optic axis of which is located coaxially to the path of jet movement in a focal point. The device and system include a detector for detection of light, focused by the objective, a logical programme, interfaced with the detector, used to determine whether a cell in the population with the cell samples is a part of required subpopulation of cells, and to output signals basing on determination whether cell is part of the required subpopulation of cells, and a controlled power source, interfaced with the logical programme, used for selective change of either cells in the required subpopulation of cells or the cells, which do not belong to the required subpopulation of cells, in accordance with a signal sent by the logical programme. |
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Red wine beverages production method Grapes (with sugar content no less than 200 g/dm3) are crushed, sulfited and exposed to vibration impact at oscillation frequency equal to 6.6-23 Hz, with amplitude equal to 1-5 mm, in an atmosphere of inert gas (carbon dioxide) under its pressure equal to 1 bar and expenditure equal to 4-28 dm3/h during 30-60 minutes. Mash is slightly fermented till sugars content in the wort is equal to 160 g/dm3 and fortified till alcohol content is equal to 16% vol. After the wine material mash infusing during 1-2 days the wine material is separated and the beverage is clarified. For fortification one uses rectified ethyl alcohol or wine distillate or rectified wine distillate or grape distillate or rectified grape distillate. |
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Double-targeted antibody in new form and using it Invention refers to biotechnology and immunology. What is presented is an antibody representing a neutralising VEGFR-2/KDR antibody with its hypervariable regions being identical to the hypervariable regions of TTAC 0001 of VEGFR-2/KDR antibody fused with a binding domain of angiopoietin 2 which is Tie-2 ligand for treating cancer by angiogenesis inhibition. A DNA coding the above antibody, an expression vector containing the above DNA, and a CHO host cell transformed by the above vector for preparing the antibody are also described. What is also presented is a method for preparing the antibody involving: host cell incubation, and the antibody recovery from a culture fluid of CHO cell. What is described is a pharmaceutical composition for treating an angiogenesis-related disease, containing an effective amount of the above antibody and at least one pharmaceutically acceptable carrier. |
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Adenoviral vectors and related methods and applications Invention refers to biotechnology and represents an oncolytic adenoviral vector, a cell and a pharmaceutical composition containing the above vector, as well as applications of the above vectors in preparing a drug for treating cancer in an individual, and to a method of treating cancer in the individual. The presented invention may be used for treating cancer. The oncolytic adenoviral vector contains a nucleic acid skeleton of serotype 5 adenovirus (Ad5), deletion in 24 nucleotides related to amino acids 122-129 in E1A constant region 2, and a nucleic acid sequence coding the human granulocyte-macrophage colony-stimulating factor (GM-CSF) in a site of deleted gp19k/6.7K in the region E3, and capside modification if needed. |
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Human immunodeficiency virus strain type 1 iv741 subtype ae for diagnostic and vaccine preparations Invention relates to virology and biotechnology. The human immunodeficiency virus strain type 1 IV741 is isolated on the territory of the Russian Federation from a patient who has not received ARV therapy. The strain is deposited in the State Collection of Viruses of the D. I. Ivanovsky Research Institute of Virology of the Ministry of Health and Social Development of Russia under number N1186. The strain belongs to HIV subtype AE. The strain has stable reproductive activity. The infectious titre is 5-6 lg TCD50. |
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Subtype b type 1 iv735 human immunodeficiency viral strain for diagnostic and vaccine preparations Invention refers to a subtype B of a human immunodeficiency viral strain, and can be used in virology, medicine and biotechnology. The presented type 1 IV735 human immunodeficiency viral strain is deposited in the State Collection of Viruses of Federal State Institution Ivanovsky Research Institution of Virology of the Russian Ministry of Healthcare and Social Development, No. 1189. The strain possesses a stable reproductive activity. An infectious titre makes 6 lg 50% tissue cytopathic dose. |
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Rationally developed media for cell cultivation Invention relates to biotechnology and represents a method of obtaining a polypeptide in a culture of mammalian cells (versions) and a medium for cultivation of the mammalian cells. The claimed invention can be used for obtaining the polypeptide of interest in great amounts. The method includes obtaining the cell culture, which contains the mammalian cells containing a nucleic acid, which codes the polypeptide of interest, and an initial medium for cell cultivation, with a volume of the initial medium for cell cultivation constituting approximately 60-99% of a volume of the desired cell cultivation medium. The nutritional medium for cultivation is added to the cell culture, with a volume of the nutritional medium for cell cultivation constituting approximately 1-40% of a volume of the cell cultivation medium. The obtained medium for cell cultivation represents a medium for cell cultivation, containing from approximately 7 mM to approximately 30 mM of leucine, from approximately 7 mM to approximately 30 mM of lysine, from approximately 7 mM to approximately 30 mM of threonine, from approximately 7 mM to approximately 30 mM of proline and from approximately 7 mM to approximately 30 mM of valine. The cell culture is kept under conditions, which allow expressing the polypeptide of interest. |
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Self-inactivated helper adenoviruses for preparing high-capacity recombinant adenoviruses Invention refers to biotechnology. What is presented is an adenovirus helper vector for preparing a high-capacity recombinant adenovirus. The invention can be used in cell technology. |
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Method of identifying improved protein versions Invention relates to a method of identifying improved protein versions. The method includes testing a multitude of protein versions with single substitutions in the first test of the first property and in the second test of the second property. The parent protein property is given a value 1.0 in each test. The favourable first or second property possesses a value, exceeding 1.0, and extremely unfavourable first or second property has a value less than approximately 0.80. The said parent protein represents protease. The first property is stability, the second property is washing efficiency. Identification of substitution and introduction of the substitution into the protein to obtain the protein with multiple substitutions are carried out. The substitution does not interact in a three-dimensional structure of the said parent protein, and one of the substitutions contains a change of the total charge, equal to 0, -1 or -2 with respect to the parent protein, and, at least, one of the substitutions contains the change of the total charge, equal to +1 or +2 with respect to the parent protein. The protein version with multiple changes in the first and second tests is tested and the said protein version is identified. |
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Application of energy sources of sun and biomass in farming Invention relates to renewable energy sources to be used in farming. This method comprises application of the Sun renewable energy source and biomass of manure, biogas and slime as organic fertilisers in greenhouses and lawn-and-garden cultures. Firs, during April-October term, greenhouse is heated by water lines heated solely by sun energy to 60…70°C at continuous hot water circulation in closed circuit "greenhouse-reactor-greenhouse" when filtered manure filtrate is fed simultaneously with production of biogas to be discharged via communication pipelines into gas-holder and to household consumers and to boiler room heater. The latter allows circulation of hot water and regulation of constant temperature in said closed circuit "greenhouse-reactor-greenhouse". At a time, bioslime, organic fertilisers, are processed and fed via pipelines into greenhouse and garden-melons-gourds plots. Then, in November-March term two intermediate modes are used. First mode is used when greenhouse is not heated due to recultivation and other works and heated is solely the manure substrate by continuous circulation of hot water in closed water lines circuit "boiler room-reactor-boiler room". Proposed biomass is used like it is at the main mode while biogas is fed to household consumers. The latter allows circulation of hot water and regulation of constant temperature in said closed circuit "greenhouse-reactor-greenhouse". Second mode allows heating of both greenhouse and manure substrate by hot water circulation in closed circuits of "boiler room-reactor-green house" and "boiler room-reactor-boiler room". Processed biogas and bioslime are used similarly to the initial main mode. Then, the latter and two intermediate modes are used again. |
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Method of determining protozoa blastocystis spp with different degrees of virulence Method comprises restriction analysis of bacterial DNA of blastocysts using a combination of restriction endonucleases - Hae III, Pst I and Hind III, which enable to identify the protozoa with different degrees of virulence. At that the use of the restriction enzyme Hae III enables to carry out the differentiation of avirulent strains of the blastocysts based on the presence of fragments with size of 850 bps, and the virulent strains based on the presence of DNA fragments with size from 550 to 10000 bps. Using the restriction enzyme Pst I the blastocysts with moderate virulence are revealed based on the presence of DNA fragments with size of about 900 bps. Use of the restriction enzyme Hind III enables to define the type of blastocysts with mild virulence based on DNA fragments with size of 700 bps, and highly virulent strains of blastocysts based on the presence of DNA fragments with size of 380 and 600 bps. |
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Phage φ-mru polynucleotides and polypeptides and their application Invention relates to the field of biochemistry, in particular to polypeptides, which are the inhibitors of methanogen cell and biological markers for detection of φmru phage, as well as polynucleotides, which code the said polypeptides. Disclosed are expression vectors and cloning vectors, which contain the said polynucleotides and host cells, containing the said vectors. Described are conjugated or fused molecules, which are the inhibitors of the methanogen cell and biological markers for detection of φmru phage, as well as antibodies, binding with the said polypeptides. Also disclosed is φmru phage, isolated with application of the described polypeptides. The invention also relates to pharmaceutical compositions and methods of inhibiting the methanogen cell with application of the described polypeptides, conjugated or fused molecules. |
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Invention provides a recombinant plasmid pESAT6-DBD consisting of the artificial bacterial operon of chimeric protein comprising a promoter region of an early promoter of bacteriophage T5, gene of the chimeric protein consisting of a sequence of protein antigen ESAT6 of Mycobacterium tuberculosis, fused with the sequence of dextran-binding domain (DBD) of dextran-sucrase Leuconostoc citreum KM20 and transcription terminator; bacterial operon of beta-lactamase and bacterial initiation site of replication of ColEl type. The invention also comprises the strain of Escherichia coli - producer of the chimeric protein ESAT6-DBD, and the method of immobilisation, concentration and purification of the resulting protein on the dextran. In addition, the invention relates to the recombinant protein ESAT6-DBD and immunogenic compositions comprising it, aiming at the induction of immunity against tuberculosis infection. |
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Pharmaceutical composition for treatment and prophylaxis of bacterial infection Pharmaceutical composition includes bacteriophages obtained by cultivation on nutritional medium containing glucose, sodium chloride, twice-substituted sodium phosphate, liquid autolysed yeast and clean water in the specified ratio, and dried and having a filler without lyophilisation, in the form of pills with a gastral-resistant coating. |
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Invention proposes an inoculation loop for cultivation of microorganisms. Bacteriological loop includes a handle on one side and a detachable working element on the other side. The working element represents a metal rod with an eyehole on its end. The working element near a coupling is equipped with metal limiters located perpendicular to the working element. Metal guides are fixed on ends of metal limiters. Guides are located along the metal rod with an eyehole at the distance from it, which is equal to external radius of test tube cross-section. |
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Bacteria strain Salmonella enteritidis var. Issatschenko 32/3 deposited in the Departmental Collection of Beneficial Microorganisms for Agricultural Purpose of All-Russia Research Institute for Agricultural Microbiology (GNU VNIISHM Rosselkhozakademii) with registration No. RCAM 00149 has expressed pathogenic properties against mouse-like rodents. Efficiency of bioattractant obtained based on Salmonella enteritidis var. Issatschenko 32/3 bacteria strain and grain in grain and vegetable warehouses and green-houses was more than 90% in relation to sewer rat and house mouse. |
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Method for modular design of DNA aptamers capable of specific and high-affinity binding of thrombin, which have stabilised basic substructure, provides for assembly of their structure by modelling using a combination of three structural modules containing a quadruplex nucleic acid module, a duplex nucleic acid module and a nucleic acid module that binds them and has a non-canonical structure by determining tertiary structure with a spectral circular dichroism method with confirmation of the fact of formation of more stable G-quadruplex that is different from quadruplex structure of initial structural quadruplex module. |
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Method of producing fatty acid methyl esters using lipase mixture (versions) Invention relates to biotechnology. Disclosed are versions of a method of producing fatty acid methyl esters (biodiesel) in a solvent-free microwater system. The method involves step-by-step addition of methanol to triglycerides in the presence of a lipase preparation and carrying out a reaction in suitable conditions until the triglycerides are converted to fatty acid methyl esters. The lipase preparation contains at least two lipases, separately or together immobilised on a porous hydrophobic support, where one of the lipases has high affinity for partial glycerides and the other is position-specific to the sn-1,3 position, and a third optional lipase has high selectivity for the glycerine sn-2 position. The support is selected from a group comprising porous hydrophobic supports based on aliphatic or aromatic polymers. Also disclosed is a method of preparing a mixture of lipases immobilised on said support. The mixture contains Candida antarctica B lipase and at least one lipase obtained from Pseudomonas sp., Alcaligenes sp., Burkholderia sp. and Thermomyces lanuginosa. |
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Invention refers to immunology, medicine and biotechnology. What is presented is a method for stimulating a specific anti-tumour immune response to breast cancer cells with the use of dendritic cells transfected by polyepitope DNA construct. The cells recovered from the peripheral blood of relatively healthy donors, transfected by the polyepitope structure are cultured with a non-adherent fraction of mononuclear cells. |
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Treatment of pigs with pcv2 antigen Invention relates to biotechnology. The method of treating or preventing a PCV2 infection or reducing clinical symptoms caused by or associated with a PCV2 infection in animals, having anti-PCV2 antibodies, involves single-step administering of an effective amount of a PCV2 antigen to the animal in need of such treatment or preventive treatment. |
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Aeromonas bestiarum bacteria strain - producer of alkaline ribonuclease having antiviral activity Invention proposes Aeromonas bestiarum bacteria strain - producer of alkaline ribonuclease having antiviral activity and deposited in a collection of bacteria, bacteriophages and fungi of the Federal Budgetary Scientific Institution "The State Scientific Centre of Virology and Biotechnology "Vector" with registration No. B-1270. Strain has high production rate of alkaline ribonuclease - 921.8 U/ml and activity against A/H5N1 bird and A/Aichi/2/68 (H3N2) human being flu viruses. |
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Invention relates to a preparation for babies for reduction or prevention of inflammation in a baby. The preparation for babies includes a source of protein, providing from 1 to 5 g of protein per 100 kkal of the preparation, a source of fat or lipids, providing from 3 to 7 g of fat or lipids per 100 kkal of the preparation, a source of carbohydrates, providing from 8 to 12 g of carbohydrates per 100 kkal of the preparation, a source of long-chain polyunsaturated fatty acids, including docosahexaenoic acid. The preparation also includes from 1×104 CFU to 1×1010 CFU of Bifidobacterium longum AH1206 NCIMB 41382 per a gram of the preparation. Also claimed are probiotic baby food and a method of reduction or prevention of inflammation in the baby or child with application of the said food. |
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Method of counting oil-oxidising bacteria in sea water Invention relates to microbiology and can be used in monitoring environmental-microbiological investigation of the quality of sea water to determine the amount of oil-oxidising microorganisms. The method involves preparing a mineral medium - bases containing NH4NO3, K2HPO4, KH2PO4, MgSO4, CaCl2, FeCl2, a concentrated solution, agar and distilled water in a given ratio, followed by addition of an oil product in a given amount, said product being bunker oil. Seeding sea water on the surface of the culture medium and incubating the seed for 3-4 hours enables to detect colonies of oil-oxidising bacteria. |
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Method for determining activity of transcription factors Method involves transfectant cells of HeLa line obtained by introduction to their chromosome of plasmid vector pBI/neo/X (X - any eukaryote transcription factor) containing minimum promoter of human cytomegalovirus, gene of green light-harvesting protein, sequence of nucleotides coding the fixation point of transcription factor, and neomycin resistance gene. Activity of transcription factor is determined by measurement of vital fluorescence intensity of the obtained cell culture in presence of test substance in comparison to intact cell culture. |
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Method of bacterial ghost (bg) Invention relates to biotechnology and represents a method of producing a bacterial ghost preparation, a pharmaceutical composition, which is a vaccine or an adjuvant, a method of inactivating live bacterial cells in the bacterial ghost preparation and application of beta-propiolactone. The method includes obtaining the bacterial ghost preparation and processing the bacterial ghost preparation with beta-propiolactone in a final concentration from 0.01% to 1% (vol/vol), at which the quantity of live bacterial cells in the said ghost preparation reduces by at least 103-104. The pharmaceutical composition includes efficient quantity of the bacterial ghost preparation, processed with beta-propiolactone in a final concentration from 0.01% to 1% (vol/vol), and a pharmaceutically acceptable carrier, a diluent and/or an adjuvant. |
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Flu virus, capable of infecting canidae and its application Invention relates to the field of biotechnology and virology. Claimed are isolated strains of the flu virus, capable of infecting canidae and cause respiratory diseases in canidae. Compositions and methods for inducing immune response against the flu virus in canidae are also described. |
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Compositions and multi-parameter assays for measuring biological mediators of physiological health Invention relates to biotechnology and presents a method of evaluating inflammation and/or insulin resistance in an animal via quantitative determination of presence of an analyte in serum or plasma. The method involves collecting a biological sample of the animal, wherein the sample contains a set of analytes, including at least a cytokine, a chemokine, a hormone and an adipokine. The sample interacts with a collection of molecular probes, separately immobilised on a multi-parameter assay panel, to determine presence of each substance from said set of analytes. For each analyte in the set, the collection of molecular probes includes at least one probe suitable for detecting presence of said analyte. Each probe is capable of producing an independently detectable signal if the analyte is present in the sample. Signals obtained from interaction of the sample with the collection are detected. A correlation is established between the signals and the presence of a substance from the set of analytes in the sample. A correlation is established between the presence of a substance from the set of analytes in the sample and known health status parameters. The health of the animal is assessed in accordance with the obtained results. |
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Invention represents Escherichia coli M15 [pREP4, pAg85A-DBD] strain - producer of Ag85A-DBD chimeric protein, as well as a method for immobilisation, concentration and cleaning of protein obtained based on dextrane. Invention relates to a method for obtaining immunogenic composition based on Ag85A-DBD recombinant protein mixed with dextrane and to Ag85A-DBD recombinant protein itself. |
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Invention relates to food industry, in particular, to production of food additives that may be used during liqueurs and spirits manufacture. The food additive is represented by a complex of the following organic acids: amino acetic (glycine), ascorbic and malic acids; the food additive recipe contains, per 100 kg of the product: amino acetic acid (glycine) - 80 kg, ascorbic acid - 12 kg and malic acid - 8 kg. |
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Invention relates to chemical-pharmaceutical industry and represents a preparation for involving a mesenchymal stem cell of the bone marrow into peripheral blood from the bone marrow, which is introduced into the blood vessel or muscle and which contains any of components: (a) protein HMGB1; (b) HMGB1 protein-secreting cell; (c) a vector, into which HMGB1 protein-coding DNA is inserted; (d) protein HMGB2; (e) HMGB2 protein-secreting cell; (f) a vector, into which HMGB2 protein-coding DNA is inserted; (g) protein HMGB3; (h) HMGB3 protein-secreting cell; and (i) a vector, into which HMGB3 protein-coding DNA is inserted. |
Another patent 2513495.
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