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Aeromonas bestiarum bacteria strain - producer of alkaline ribonuclease having antiviral activity. RU patent 2520086. |
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FIELD: biotechnologies. SUBSTANCE: invention proposes Aeromonas bestiarum bacteria strain - producer of alkaline ribonuclease having antiviral activity and deposited in a collection of bacteria, bacteriophages and fungi of the Federal Budgetary Scientific Institution "The State Scientific Centre of Virology and Biotechnology "Vector" with registration No. B-1270. Strain has high production rate of alkaline ribonuclease - 921.8 U/ml and activity against A/H5N1 bird and A/Aichi/2/68 (H3N2) human being flu viruses. EFFECT: improving strain properties. 2 dwg, 7 tbl, 8 ex
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Method of counting oil-oxidising bacteria in sea water / 2520084 Invention relates to microbiology and can be used in monitoring environmental-microbiological investigation of the quality of sea water to determine the amount of oil-oxidising microorganisms. The method involves preparing a mineral medium - bases containing NH4NO3, K2HPO4, KH2PO4, MgSO4, CaCl2, FeCl2, a concentrated solution, agar and distilled water in a given ratio, followed by addition of an oil product in a given amount, said product being bunker oil. Seeding sea water on the surface of the culture medium and incubating the seed for 3-4 hours enables to detect colonies of oil-oxidising bacteria. |
Strain of rhodococcus sp-destructor of petroleum hydrocarbons / 2518349 Strain of Rhodococcus sp. is deposited in the All-Russian Collection of Microorganisms under the registration number Ac-2046 D. The strain exhibits high destructive activity against petroleum hydrocarbons included in the composition of oil slurries, as well as against crude oil and black oil fuel. |
Nutrient medium for submerged cultivation of tularemia microbe / 2518282 Nutrient medium for submerged cultivation of tularemia microbe contains as a base, that provides the content of amino nitrogen in the medium of not less than 0.32%, dry enzymatic hydrolyzate of fibrin obtained from waste of whey-vaccine production, and the components - glucose and calcium pantothenate at a predetermined ratio. |
Nutrient medium for extraction of bacteria of shigella kind from water objects / 2517750 Nutrient medium is proposed for extraction of Shigella kind bacteria from water objects. The nutrient medium contains the following components: 0.1% alcohol solution of bromcresol green - 9.0 ml; 0.2% alcohol solution of methyl red - 3.0 ml; fermentative peptone - 0.5 g; extract of fodder yeast - 4.5 g; potassium dihydrophosphate - 8.7 g; caustic soda - 1.4 g; sodium chloride - 5.0 g; distilled water - up to 1000 ml; medium pH is 6.70-6.85. |
Method to prepare medicated product from live strains and microorganisms of lactobacilli and bifidobacteria "lb-complex plus" / 2517734 Method provides for cultivation at 37±1°C of strains of lactobacilli and bifidobacteria in the medium and packing of liquid product with account of daily dose necessary for patients. The medium contains components in the following quantities: caseine hydrolysate dissolved by distilled water, 0.33-0.4 g/l, sodium chloride 5 g/l, fructose 10 g/l, peptone 2 g/l, agar-agar 0.75 g/l for lactobacilli, for bifidobacteria - 1.0 g/l, ascorbic acid 0.25 g/l, distilled water 0.67-0.6 g/l. Strains-producers are Lactobacillus plantarum 8 RA-3, Lactobacillus fermentum 39, Lactobacillus fermentum 90 TC-4, Bifidobacterium bifidum 791, Bifidobacterium longum 379, Bifidobacterium bifidum 1. For lactobacilli and bifidobacteria they prepare accordingly media with different content of agar-agar, amine nitrogen in caseine hydrolysate 160-170 and 180-200 mg% and medium pH 7.8-8.0 and 8.5-8.6. Starting from the first generation the bifidobacteria and lactobacilli are cultivated for 24±1 hours, two strains together, one separately. The produced biomass is mixed with the fresh nutrient medium at the ratio of 1:1000, and strains of lactobacilli are cultivated 24±1 hours, strains of bifidobacteria - 48±1 hours. Upon completion of cultivation of biomass of strains they are mixed at the ratio of 2:1:2:1. |
Nourishing composition, including probiotics and improving sleep pattern / 2517616 Invention relates to application of probiotic bacterial strain for production of a probiotic composition for reducing sleep disorders and/or improvement of sleep quality in people and animals. As a bacterial strain used is Lactobacillus reuteri DSM 17938 or Bifidobacterium longum NCC 3001 (ATCC BAA-999). |
Preparation for purification of water and soil from oil pollution and method of obtaining thereof / 2516412 Group of inventions relates to field of biochemistry, ecology, protection of the environment. Claimed is preparation for purification of water and soil from oil pollution, which contains microorganisms, oil destructors, sorbent, cryoprotector - glycerol, microfertilisers - sodium nitrate 0.5% and potassium phosphate 0.5%. As oil destructor, it contains association of oil-oxidising microorganisms: Bacillus subtilis VKM B-81, Pseudomonas spp. VKM B-892, Pseudomonas putida VKM B-1301, Rhodococcus sp. VKM Ac-950, Mycobacterium flavescens VKM Ac-1415 in amount 75-85% of the total number of cells, as well as soil bacteria Agrobacteium radiobacter VKM B-1219 in amount 15-25% of the total number of cells. Sorbent in preparation represents finely-dispersed dehydrated zeolite with size of granules 0.1-0.5 mm, powdered with Aerosil A-300 nanoparticles. Ratio of components in preparation (wt %) is the following: zeolite - 94±1, Aerosil A-300 - 3±0.5, glycerol - 1±0.2, sodium nitrate - 0.5±0.2, potassium phosphate- 0.5±0.2, association of oil-oxidising microorganisms with Agrobacteium radiobacter in efficient quantity 2-3*108 kl/g - 1±0.5. Also claimed is method of preparation obtaining. Assocoation of oil-oxidising bacteria and fraction of soil bacteria are grown separately. After that, two obtained culture liquids are mixed in ratio 75-85% of association of oil-oxidising bacteria of the total quantity of cells and 15-25% of soil bacteria of the total quantity of cells. Suspension is concentrated to concentration 2*1011 kl/ml. Zeolite is preliminarily crushed to granules with size 0.1-0.5 mm, kept in furnace at temperature 250°C to the stage of swelling, cooled to temperature 20°C at humidity 10-12% and mixed with Aerosil A-300. Glycerol, sodium nitrate, potassium phosphate are introduced into concentrated suspension, and after that mixed with zeolite. Obtained zeolite and concentrated suspension are mixed with ratio 9:1. After that process of contact-chemical dehydration is carried out. |
Hybrid protein having prolonged action, based on recombinant human interferon alpha-2 (variants), method of its production and strain of saccharomyces cerevisiae for implementing this method (versions) / 2515913 Hybrid proteins GFN80 and GFN100 are formed based on recombinant human interferon alpha-2 fused on the N-terminus with the amino acid sequence of polypeptide S(G4S)16 or S(G4S)20, respectively. The strains of producer Saccharomyces cerevisiae RNCIM Y-3927 and Saccharomyces cerevisiae RNCIM Y-3928 are produced by recombinant method. The strains are used in the method of production of the hybrid protein GFN80 and GFN100, which comprises culturing under suitable conditions of yeast cells transformed by the expression vector, which contains the region of replication initiation of endogenous 2-micron plasmid of yeast Saccharomyces cerevisiae, and the promoter of yeast GAL1 controlling the expression of the gene comprising the DNA sequence SEQ ID NO:1 or SEQ ID NO:2, respectively, followed by isolation of the hybrid protein from the culture fluid. |
Strain of fungus stagonospora cirsii davis having herbicidal activity against canada thistle / 2515899 Strain of the fungus Stagonospora cirsii Davis of All-Union Research Institute of Plant Protection 1.42 has herbicidal activity against canada thistle and species closely related to it - S. Cirsii. It was deposited in the State Collection of Microorganisms of All-Union Research Institute of Plant Protection under the collection number of Stagonospora cirsii Davis of All-Union Research Institute of Plant Protection 1.42 and can be used for biological control of canada thistle. |
Medication (versions), composition (versions) and application of medication (versions) for reduction of halitosis / 2515113 Invention relates to versions of medication for reduction of halitosis, versions of compositions based on said medications and versions of application of said medications. Device for reduction of halitosis represents strains of microorganisms Lactobacillus acidophilus, selected from group Lactobacillus acidophilus DSM 19825, Lactobacillus acidophilus DSM 19826 and Lactobacillus acidophilus DSM 19827. Version of said medication represents culture supernatant of said strains. Also claimed are compositions, which contain said microorganisms, and their application for reduction of halitosis. |
Structure based on protein pair of barnase-barstar and method of its production / 2480524 Method is proposed to develop the specified structure, which includes: a) development of at least two modules, where each module is independently made in the form of a nucleus that carries molecules of the first protein and the second protein on the surface, where the first protein is barnase, and the second protein is barstar, besides, the first or second protein for binding with each module is selected in the following manner: the first module is made as carrying protein molecules on its surface selected from the first protein or the second protein; the second module is performed as carrying protein molecules on the surface selected from the first protein or the second protein and different from the protein contained in the first module; the third and each subsequent module carries molecules of either the first or the second protein on the surface; b) combination of the first, second and subsequent modules, in which modules are self-assembled in the structure due to interaction of the molecules of barnase and barstart, besides, nuclei of at least two of the specified modules are made with particles of supermolecular nature. The structure is described for diagnostics and therapy produced by the specified method. |
Controlled degradation of structured polyribonucleotides / 2458986 Structured polyribonucleotides undergo degradation through incubation of a sample containing them with thermally stable polynucleotide phosphorylase (PNPhase) Thermus thermophilus in the presence of a co-substrate and a cofactor. |
Method for production of pancreatic ribonuclease / 2388821 Method provides for grinding of pancreas. Ground pancreas is treated with solution of sodium chloride of 4.5-6 wt % in the following volume ratio between pancreas and salt solution equal to 1:(1.5-2.0) at the temperature of 25-35°C for 2.5-3.5 hours. Produced cells of pancreas are separated by centrifugation and treated with 95% ethyl alcohol for 15-24 hours in volume ratio of 1:(0.85-1.3) at the temperature of 15-25°C. Pancreas cells are separated by centrifugation with further treatment of these cells with water acidified with hydrochloric acid to pH of 1.0-3.0 for 2.5-4.5 hours at the temperature of 25-35°C in volume ratio of 1:(0.8:1.2). Produced cells of pancreas are separated by centrifugation to produce supernatant. Ribonuclease is extracted from produced supernatant by solutions of sulfuric acid with concentration of 0.15-0.35 for 3-4 hours with volume ratio of 1:(0.8-1.2) at the temperature of 4-6°C. Suspended particles are separated by centrifugation to produce extract that contains ribonuclease. Produced extract is concentrated by ultrafiltration on UPM 10 membrane with further diafiltration. Produced concentrate is cooled down to 4-6°C, and ribonuclease is deposited at pH of 8.0-9.0. Produced residue is washed with ethyl alcohol in volume ratio of 1:5 and dried. |
Streptomycin-resistant strain bacillus sp arcim b-9862, producer of extracellular alkaline ribonuclease / 2384619 Strain Bacillus sp. ARCIM B-9862 is a high-stable producer of alkaline extracellular ribonuclease with high resistance to streptomycin; it is deposited in the All-Russian Collection of Industrial Microorganisms (ARCIM), Federal State Unitary Enterprise State Research Institute for Genetics, number B-9862. |
Method of preparing cholesterol-esterase, tripsin, deoxyribonuclease, and ribonuclease from bovine pancreas / 2311455 Invention aims at biotechnological preparation of following medicaments: pancreatic ribonuclease, pancreatic deoxyribonuclease, tripsin, and cholesterolesterase. Method envisages grinding bovine pancreas and homogenation thereof. Resulting homogenate is extracted with cooled ethanol at constant stirring, centrifuged, and subjected to second extraction, now with sulfuric acid containing magnesium sulfate, at constant stirring. Extract containing desired enzymes is separated from precipitate via centrifugation. Ballast proteins are precipitated by ammonium sulfate at 30-35% saturation and removed from supernatant via centrifugation. Cholesterolesterase is precipitated by ammonium sulfate at 45-50% saturation of extract, tripsin at 60-65% saturation, and DNA-ase at 95-98% saturation followed by dialysis and chromatographic purification on DEAE-cellulose column. |
Oligonucleotide-peptide conjugate with ability to cleave rna phosphodiester bonds in 5'gpn3' sequences / 2305108 Disclosed is oligonucleotide-peptide conjugate of general formula 3'TTCTCTAGG5'-dRib-dRib-dRib-N-terminal-Leu-Arg-Leu-Arg-Leu-Arg-Arg-Gly-NH2-C-terminal (wherein T is thymine residue; C is cytosine residue; A is adenosine residue; G is guanosine residue; drib is deoxyribose residue; Leu is leucine residue; Arg is arginine residue; Gly is glycine residue). Said conjugate cleaves phosphodiester bonds in single-stranded RNA sites in 5'GpN3' (wherein G is guanosine; N is any ribonucleotide) sequences only. |
Completely balanced in nutritional value preparation for babies, probiotic baby food and method of reduction or prevention of inflammation in baby or child / 2520085 Invention relates to a preparation for babies for reduction or prevention of inflammation in a baby. The preparation for babies includes a source of protein, providing from 1 to 5 g of protein per 100 kkal of the preparation, a source of fat or lipids, providing from 3 to 7 g of fat or lipids per 100 kkal of the preparation, a source of carbohydrates, providing from 8 to 12 g of carbohydrates per 100 kkal of the preparation, a source of long-chain polyunsaturated fatty acids, including docosahexaenoic acid. The preparation also includes from 1×104 CFU to 1×1010 CFU of Bifidobacterium longum AH1206 NCIMB 41382 per a gram of the preparation. Also claimed are probiotic baby food and a method of reduction or prevention of inflammation in the baby or child with application of the said food. |
Method of counting oil-oxidising bacteria in sea water / 2520084 Invention relates to microbiology and can be used in monitoring environmental-microbiological investigation of the quality of sea water to determine the amount of oil-oxidising microorganisms. The method involves preparing a mineral medium - bases containing NH4NO3, K2HPO4, KH2PO4, MgSO4, CaCl2, FeCl2, a concentrated solution, agar and distilled water in a given ratio, followed by addition of an oil product in a given amount, said product being bunker oil. Seeding sea water on the surface of the culture medium and incubating the seed for 3-4 hours enables to detect colonies of oil-oxidising bacteria. |
Method of determining sensitivity of microorganisms of salmonella genus to antibacterial agents / 2518372 Test kind of microorganisms of the genus Salmonella is incubated, which is taken at a final concentration of approximately 1000 cells/ml, with the test antibacterial agent in the known concentration previously specified for each antibacterial agent, which is optimal for exposure to the specified kind of microorganisms. Incubation is carried out at a temperature of 37°C for 24 hours. The samples are added to the aptamers specific to test species of living microorganisms, fluorescently labelled, at a final concentration of 100 nM. It is incubated for the second time for 20 minutes. The sample is examined using flow cytometry. The level of fluorescence of aptamers is determined by graphs made with a flow cytometer using a program which enables to display a percentage value. The part of bound and un-bound bacteria with the aptamers fluorescently labelled is determined. The amount of the living or non-living organisms in the sample is determined. |
Strain of rhodococcus sp-destructor of petroleum hydrocarbons / 2518349 Strain of Rhodococcus sp. is deposited in the All-Russian Collection of Microorganisms under the registration number Ac-2046 D. The strain exhibits high destructive activity against petroleum hydrocarbons included in the composition of oil slurries, as well as against crude oil and black oil fuel. |
Two-phase nutritional medium for thin layer cultivation of helicobacter pylori and method of its realisation / 2518304 Invention relates to field of microbiology and can be applied in medicine. Method includes preliminary inoculation of analysed material on Columbia agar with addition of 5% of sheep blood. Thermostatting is performed under microaerophilic conditions in CO2 incubator at 37°C for 3-5 days at higher humidity. Sampling colonies and their re-inoculation on Petri dishes with chocolate agar are performed and thermostatting is carried out under microaerophilic conditions in CO2 incubator at 37°C for 3-5 days at higher humidity. When thermostatting stage is completed Petri dishes with chocolate agar are poured on the top with liquid nutritional medium based on Schaedler broth, enriched with 10% cattle serum with their further thermostatting in CO2 incubator at 37°C for 72 hours. |
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