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Method of year-round breeding of predatory bug perillus (perillus bioculatus fabr) Method comprises management of perillus bug under optimum conditions using natural feed. At that the feed is used as bee moth larvae previously frozen at a temperature of (-5)-(-7)°C for 3-4 hours, and the mature zooids of perillus are subjected during breeding to cooling for 30 days at a temperature of 4°C. |
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Method of breeding common greenbug (schizaphis graminum rond) Method of breeding common greenbug comprises sowing seeds, growing fodder plants, greenbug infestation of plants, collection and accounting of greenbugs. At that the seeds before sowing are processed with plant growth stimulant. |
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Method of fertility improvement of eastern mink and viability of offspring Invention is intended to increase the fertility of eastern mink Standard (+/+ +/+), and the viability of its offspring. The method comprises the use of the agent Biostim. Biostim is used for females and males prior to oestrum for 5 days and during the oestrum three times every second day at a dose of 0.05 ml/kg body weight. |
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Method of improvement of reproductive function of cows and vitality of new-born calves 5 ml of 1% solution of sodium selenite containing 1 ml of alcoholic extract of Acanthopanax sessiliflorum per 100 ml of solution is administered to non-milking intramuscularly with. The solution is administered once for 30-20 days prior to proposed calving. |
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Invention relates to veterinary medicine. For growing calves, the stimulating mineral feed additive is added into the diet, which is used as a preparation of micellae of calcium carbonate "Alexanat Zoo". The preparation solution is given to drink daily for 60 days in a dose of 10 ml per head. Before administering to calves the additive is diluted in two litres of milk. The preparation "Alexanat Zoo" of grade A and density of 1400-1500 g/l is used. |
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Antibody-producing non-human mammals Invention relates to field of biotechnology. Claimed is transgenic animal, representing mouse or rat, into whose genome inserted is nucleic acid, coding rebuilt variable region of light human immunoglobulin chain, functionally connected with constant region of said animal immunoglobulin and functionally connected with promoter, enabling expression of transgenic light chain in mammalian B-cells in such a way that it forms pairs with different endogenic heavy chains, enabling production of antibodies. Methods of producing antibodies with application of transgenic animal, B-cell of transgenic animal, application of animal and its B-cell for production of antibodies and method of obtaining transgenic animal in accordance with invention are also claimed. |
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Method of growing entomophages chouioia cunea jang Invention relates to the biological method of protection of forest stands and can be used in the technology of mass obtaining the entomophage culture for the subsequent release into the sources of forest pests. The proposed method comprises the use of insect-host, in which entomophage Chouioia cunea Jang develops, as pupae of emperor moths Samia cynthia ricini (Boisduval, 1854). |
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Method comprises feeding the animals during transportation and preslaughter treatment 5 days prior to transportation with a mixture of ammonium perchlorate and mival-Agro at a dose of 40-50 mg per 1 kg of body weight at a ratio of 1:8. |
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Method of determining viability of new-born piglets Method comprises determining the parameters of temperature and body weight within the first 24 hours of life. The body temperature is measured after birth (To.b) and after 1, 2, 4, 6, 12, 24 hours (To.), and the body weight after birth (Mo.b) and after 24 hours (Mo.). Then the mean value of the body temperature of each animal in terms of time is calculated, and 1-24 hours after birth ( X ¯ T o . ) , the mean value of the body temperature for a group of animals at birth ( X ¯ T g r .b ) and 1, 2, 4, 6, 12, 24 hours after the birth ( X ¯ T g r . ) . Similarly, the mean values of the body weight for a group of animals at birth are calculated ( X ¯ M g r .b ) , and 24 hours after birth ( X ¯ M g r . ) . According to the results of mean values of temperature and body weight the index of viability (IV) is calculated. If the value of IV>2 the new-born piglets are referred to as viable, if IV<2 they are referred to animals with low viability. |
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Biologically active stimulant for poultry Invention relates to the poultry husbandry and can be used to stimulate the growth of poultry, increase in egg production. The proposed biologically active stimulant for poultry husbandry is an aqueous-alcoholic extract of the vegetative part of plants - Acanthopanax sessiliflorum and Patrinia scabiosifolia, taken in equal amounts. |
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Invention relates to dairy farming, in particular to methods of forming the breeding herd of Holstein cows under conditions of monsoon climate. Every year in the breeding herd the cows born in the summer are inseminated in time which does not enable to carry out their calving during the summer months, and cows-top producers are inseminated in time which does not enable to carry out their calving in autumn, at that the first calving of the heifers received from them is also carried out also in autumn. |
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Method of activating reproductive function of pigs Method comprises adding to the basic diet of the agent of activation of the reproductive function of pigs, 100 g of which contains: vitamin A (retinol acetate) - 100 thousand ME; vitamin D3 (cholecalciferol) - 10 thousand ME; vitamin E (α-tocopherol acetate) - 60 mg; vitamin B1 (thiamine) - 140 mg; vitamin B2 (riboflavin) - 130 mg; vitamin B3 (niacinamide), - 400 mg; vitamin B5 (calcium pantothenate) - 320 mg; vitamin B9 (folic acid) - 120 mg; vitamin B12 (cyanocobalamin) - 400 µg; vitamin C (ascorbic acid) - 400 mg; K2HPO4 - 35 g; NaHSO4 - 5 g; NTF salt with trace elements (Fe - 1600 mg; Cu - 1000 mg; Zn - 3000 mg; Mn - 1700 mg; Co - 350 mg); lysine - 20 g; methionine - 20g; cysteine - 10 g. The agent is given in the doses: 0.3% to the daily rate of feed - in the first 84 days of pregnancy; 0.4% - from the 85th day to 114-115th day of pregnancy. |
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Method of reduction of production losses of gobies at transportation and preslaughter stress Method comprises feeding gobies with the preparation energosyl 7 days prior to transportation to a dose of 30-50 mg/kg body weight. |
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Method of predicting milking capacity of holstein first-calf heifers of different lines Method of predicting milking capacity of Holstein first-calf heifers of different lines comprises the selection of the first-calf heifers by determining the productivity index. At that the average amount of milking of a line of the first-calf heifers for 1 lactation and their average body weight during this period is determined, and the index is used as the milking coefficient which is defined by formula: M C = Y L W × 100 ; where Y is the milk yield for 1 lactation (kg); LW is an average live weight (kg), in case the milking coefficient is 600 and less the line of the first-calf heifers is referred to low-productive, if the coefficient is 600-1000 - to productive, and if the coefficient is more than 1,000 - to highly productive ones. |
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Method of breeding stiff-tailed ducks oxyura leucocephala For breeding stiff-tailed ducks Oxyura leucocephala the birds are managed during the reproduction period in areas of habitat with nesting, the breeding stock is fed daily with a mixture of plant and animal feeds, lekking is carried out, mating of the birds, egg-laying and hatching eggs to hatching the nestlings, the nestlings are fed with adult females. The areas of habitat of the stiff-tailed ducks with nesting during lekking, mating, laying eggs by a female, brooding in nests and rearing of the nestlings under the female are paired cages designed for one pair of birds and their offspring, each of which consists of a pool of water, a feeding site and a nesting niche. For breeding the mature pairs of birds with males are selected, which have feathers mewed into the breeding colour. The males are removed from the females into an adjacent room of the paired cage after laying several eggs or at the beginning of brooding. When growing hatchlings, which are with the female in the cage, during the first 7-10 days of their lives the live feed is placed in the pool with water: daphnia or bloodworm, and plants such as duckweed and pondweed which serve as food for the ducklings followed by their gradual transfer to feeding with a mixture of feed for young birds and adults. By 4 weeks of age the nestlings are transferred for management to the cages for the breeding stock where they are managed until autumn separately from the adult birds. Feeding the birds is carried out at least 7 times a day with a mixture of the feed for the young and adult birds, containing steamed cereal of grains, minced boiled meat, minced boiled fish, dry gammarus, boiled chicken egg, finely grated fresh carrots, finely cut fresh herbs, nettle flour, shells or shell rock, coarse sand, vitamins "Ultravit". |
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Invention relates to field of biotechnology, immunology and genetic engineering. Genetic modification contains deletion of endogenic low-affinity FcγR locus, where contain functional FcRγ-chain. Modified mice express up to five genes of low-affinity human FcγR on antigen-presenting cells of host immune system. Also described is method of screening substance, containing Fc site of human antibody by means of said mice. Invention can be used in the field of medicine. |
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Method for increasing reproductive ability of seed bulls Invention refers to veterinary medicine. A method provides feeding the bulls with stimulating mineral feed additive daily. An aqueous solution of the preparation Alexanate, micellate of calcium carbonate 300 g per a head for three months; before administering, the preparation is dissolved in water in ratio 1:50. |
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Method of mass rearing ichneumons minotetrastichus frontalis (nees) Method of mass rearing ichneumons Minotetrastichus frontalis (Nees) is proposed. The method comprises the leaves of linden, used as fodder resources for rearing ichneumons, containing mining damages with caterpillars and/or pupae of linden leaf blotch miner Phyllonorycter issikii (Kumata). The caterpillars and/or pupae of linden leaf blotch miner Phyllonorycter issikii (Kumata) are placed in a single layer in a plastic transparent container with the size l×w×h (cm): 50×20×20, with the ventilation openings which are not more than 0.1 mm on an area of 400 cm2. In a container the fertilized females of ichneumons Minotetrastichus frontalis (Nees) are placed at the rate of 1 female per 10 caterpillars and/or pupae of linden leaf blotch miner Phyllonorycter issikii (Kumata). They are kept for 72 hours under constant temperature conditions of 18-22°C. |
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Method of increasing reproductive qualities of sows Method comprises adding to the basic diet of agent of increasing the reproductive qualities at a dose of 0.3% of the weight of daily rate of feed in the first 84 days of pregnancy and at a dose of 0.4% from the 85th day to 115th day of pregnancy, 100 g of which contains: Vitamin A (retinol acetate) - 100 thous. ME; Vitamin D3 (cholecalciferol) - 10 thous. ME; Vitamin E (α-tocopherol acetate) - 50 mg; Vitamin B1 (thiamine) - 100 mg; Vitamin B2 (riboflavin) - 110 mg; Vitamin B3 (niacinamide) - 420 mg; Vitamin B5 (calcium pantothenate) - 350 mg; Vitamin B9 (folic acid) - 100 mg; Vitamin B12 (cyanocobalamin) - 450 µg; Vitamin C (ascorbic acid) - 400 mg; KH2PO4 - 50 g, oxyethylidenediphosphonic acid salts with trace elements (Fe - 1600 mg; Cu - 1000 mg; Zn - 3000 mg; Mn - 1700 mg; Co - 350 mg), lysine - 20g, methionine - 20 g. |
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Invention relates to the field of agriculture. The crossbred offspring is obtained by mating ewes of Stavropol fine-wool breed of wool production direction with stud rams of Volgograd fine-wool breed of meat and wool production direction. The rams of the offspring obtained at the age of weaning them from the ewes, of 4-4.5 months, are divided into groups depending on the degree of development of the horn length. The rams (lambs) are assessed based on productivity indices, using as a marker of the productivity index the degree of their hornedness. On the basis of the assessment the prediction is created based on the productivity indices "live weight", "wool shearing" by the age of 14-15 months. |
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Method of evaluation of beef cows on milk yielding capacity Method of evaluation of beef cows on milk yielding capacity is characterised by estimated figure of milk yielding capacity, equal to the value of live weight of suckling calves at the age of 3 months, received per 100 kg of live weight of nursing cows of the I calving in this period, for cows of early-maturing beef breeds such as Kazakh white-headed - to 19.7 kg, the cows of large beef breeds such as Charolais - up to 21.5 kg, and indicating low, and the rates of the abovementioned - the higher milk yielding quality of cows of beef production. |
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Method of stimulation of poultry growth Method for stimulation of poultry growth is proposed, using 0.01% aqueous solutions of derivative amide of fatty acids (lauric and myristic) in the form of salts with pharmaceutically acceptable mineral and organic acids. The solutions are given freely instead of water for 15-18 days. The substances proposed to use are biodegraded in the body and in the environment due to the easy proceeding amide bond hydrolysis, which leads to formation of harmless fatty acid, and also N,N-dimethyl-1,3-diaminopropane, easily oxidised to simple harmless compounds. |
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Method of determining biological activity of embryonated eggs of trichuris Invention deals with method of determining biological activity of embryonated eggs of Trichuris. Characterised method includes realisation of at least 3 analyses, selected from: evaluation and/or confirmation of stage of embryonated development of eggs by means of method of quantitative PCR with application of suitable marker sequences for determination of quantity of genome DNA copies, evaluation of metabolic activity of embryonated eggs by means of biochemical and/or molecular-chemical methods, evaluation of inducibility of gene expression in embryonated eggs, evaluation of mobility of Trichurs larvae by means of microscope for long periods of observation after preliminary incubation at higher temperatures and/or evaluation of coefficient of hatchability of Trichurs larvae in organism of laboratory animal. |
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Method of joint growing of aphidoletes aphidimyza and aphidius colemani Invention relates to biotechnology, in particular to methods of growing Aphidoletes aphidimyza and Aphidius colemani, and can be used for biological protection of close soil plants against pest plant louse. Joint and simultaneous feeding and reproduction of Aphidoletes aphidimyza and Aphidius colemani on Aphis fabae on one area in greenhouse is carried out at temperature 10-20°C and relative air humidity 50-70%. Growing bean plants, invasion and reproduction of Aphis fabae on bean plants is carried out by analogy with "green conveyor". Intended area is divided into several fields and sowing is carried out after one day with dry seeds into specially prepared soil. Aphidoletes aphidimyza and Aphidius colemani settle on bean plants with Aphis fabae, which provides feeding and reproduction of Aphidoletes aphidimyza and Aphidius colemani together and simultaneously. |
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Method of increasing safety of young cattle Method consists in applying to pregnant cows 30 days prior to calving of immunostimulator "Ribotan" intramuscularly at a dose of 10 ml per animal once combined with the carbohydrate-mineral additive "Felucen" daily with the fodder based on 50 g per animal. |
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Producing poultry and other animals resistant to viral diseases Invention refers to biotechnology and genetic engineering. A cell contains a construct, which comprises a sequence coding siRNA to a conserved region of a foot-and-mouth disease virus (FMDV) or avian influenza virus. What is disclosed is producing a transgenic vertebrate, other than a human from the above cells, which possess the resistance to the above viral diseases. The invention can be used in animal production and veterinary science. |
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Method of determining annual meat productivity of beef cows charolaise Method is presented as the sum of data of live weight of two weaned steers and one heifer, their weight at birth and weight of culled cows after feeding, reduced with the rate of a year, and represented by the formula: Pc≈0.19·[Pcow+1.7(Pcalf+Pas··Ts)+0.85(Pcalf+Pah·Ts], where Pc is average year meat productivity of beef cows Charolaise; Pcow is mass of culled cow after feeding, kg; Pcalf is calf weight at birth, kg; Pas is average daily gain of steers at foot, kg; Pah is average daily gain of heifers at foot, kg; Ts is sucking period of calf rearing, day; 0.19, 1.7 to 0.85 is corrective breeding and technological factors. |
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Method of management and feeding dairy cows Method of management and feeding dairy cows provides for formation of groups and feeding the animals, taking into account changes in daily milk yield and body weight as the change in the physiological state of the cows in lactation period. At that, the daily milk yield is calculated based on the month of lactation and the planned milk yield per lactation. The change in body weight is calculated taking into account the number and the period of lactation, the planned milk yield per lactation, the daily increment and duration of pregnancy. The diets are calculated for the middle of each period. |
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Method of introduction of artificially reared birds to wild life Method of introduction of artificially reared birds to wild life comprises performing lekking, egg laying, and brooding eggs until hatching of chicks, feeding them and release into their proposed habitat with their mothers. For introduction the tetraonidae birds are used, such as birds of species of Siberian spruce grouse or capercaillie. Females intended for reproduction obtain natural food, such as pine needles, berries, twig food, arthropods, along with technology feeds, such as grain mixture, combined fodder, premix. Lekking, egg laying, and brooding eggs in nests with use of females until hatching is performed in aviaries. The chicks after hatching from eggs and before their introduction into the wild life are fed with live food in the form of insects that wild chicks of this species eat in the natural environment. Introduction of brood of chicks in wild life is carried out in the age of 1-3 days after hatching from the eggs directly from the aviaries with their mothers or after their transportation to the permanent habitat. And the female and brood of chicks are transported in different cages without visual contact with the ability of sound communication, and before the release of chicks and female in wild life, they are placed in one adaptational cage in which the female settles down and takes its chicks under it for heating, after which the cage is opened and the brood with the female goes away freely to the selected area of terrain, which enables to adapt to natural environment. |
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Method of flow-line production of lamb Invention relates to sheep husbandry. The method comprises management of ewes and their cyclical insemination in a timely manner and obtaining three lambing in two years. And the flock is formed from highly-forward and mid-duration meat breeds of sheep, as well as homegrown meat-wool and wool-meat breeds; the ewe flock is divided into two groups; artificial insemination of the ewes is carried out at intervals of 4 months during 20 days; the lambs are grown with the ewes for up to 2 months; then the lambs are grown in stable system; and fattening of lambs is carried out taking into account two age groups: 2-4 months and older than 4 months; selling of lambs is carried out for 4 months by age: at the age of 4 months - 20%; at the age of 5 - 25%; at the age of 6 - 30% and at the age of 7 - 25%. |
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Method of evaluating genetic potential of sheep at early age Method comprises selection of ewes taking into account the extent of their genetic compatibility with ram-breeder on the amount of index of antigenic similarity and weight indices of placenta. At that the fetal-placental index is additionally determined taking into account the ratio of weight indices of placenta and the new-born animal with the level of organometric indicators of umbilical cord depending on the degree of genetic differences of parental couple. The fetal-placental index is determined in grams by dividing the weight of a new-born animal by the weight of the placenta, the level of organometric indicators of umbilical cord is determined by the following parameters: the average diameter of the umbilical cord (ADUC d cm)=d1+d2+d3+…d5:n; the volume (V cm3)=π×r2×L (cm3); unit of volume weight (UVW g/cm3)=M:V (g/cm3); unit of linear weight (ULW g/cm)=M:L (g/cm); standard mass index (SMI g)= ULWx50 cm (g). At that the selection of ewes is carried out on the value of antigenic similarity index between 0.31 and 0.60. |
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Screening method using thalidomide-targeting factor Invention relates to a method of screening a non-teratogenic substance comprising bringing a test substance into contact with cereblon or a fragment of cereblon, evaluating the capacity of the test substance to bind to cereblon or the fragment of cereblon, and selecting a test substance that does not bind to cereblon or the fragment of cereblon or a test substance exhibiting lower capacity to bind to cereblon or the fragment of cereblon compared with thalidomide. |
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Method of production of sheep embryos in vitro Method comprises extracting the oocytes-cumulus complexes from ovaries, cultivating them to the stage of metaphase II, co-cultivating of oocytes and sperm cells, cultivating the impregnated ootids. And additionally the follicle-stimulating and luteinising hormone is administered in the form of the combined preparation Menopur, estradiol, heparin, caffeine and antibiotic. The cultivating is carried out in the base medium. Extraction of the oocytes-cumulus complexes from ovaries is carried out from live sheep under anaesthesia by puncturing ovarian follicles during laparotomy. Superovulation stimulation is preliminary carried out. In the maturation medium the preparation Menopur is added, the concentration of both components that are part of the preparation, is 0.075 IU/ml, and estradiol is added to the maturation medium in the concentration of 10 mcg/ml. For impregnation the newly obtained sperm of tups is used. For tup sperm capacitation and to the maturation medium the combination of heparin is added in an amount of 5 units/ml and caffeine in an amount of 0.2 mg/ml. The cultivation and impregnation is carried out in four well Petri dishes in 500 mcl nutrient medium under 200 mcl paraffin oil. |
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Method for creating transgenic animals with stable and high target protein expression in milk Invention refers to biotechnology, particularly to methods for preparing next generation drug preparations and biologically active additives in bioreactors on the basis of transgenic producing mammals. The method for creating transgenic animals producing a protein with stable and high expression in milk, involves producing transgenic mammals with using a vector containing a reporter gene coding a target protein that is a goat beta-casein gene promoter, a bovine growth factor terminator and effective two-fold transcription terminators. The terminators surround an expression cartridge and possess an ability to break genome transcripts effectively in a mammalian genome by the effective protection of transgene expression in the mammalian genome against further repression. The effective two-fold terminators represent any mammalian genome site fulfilling the following conditions: 3'-sites of the two simultaneously expressing and opposite genes containing a site of the second-to-last exon, the last intervening sequence, the last exon and a polyadenylation signal, a space of two polyadenylation signals at different DNA strands is no more than 100 base pairs. |
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Method of selection of parent pairs in sheep production Method comprises individual testing on blood groups of both parents. Selection is then carried out taking into account the extent of their genetic differences, expressed in terms of antigenic similarity index ranging from 0 to 1. At that the morphologic condition of placenta is additionally determined, and the selection of parent pairs is carried out with the genetic compatibility of average index values of antigenic similarity in the range of Ra=0.31-0.60 and with the morphostructural condition of placenta weighting 310-330 g, the number of cotyledons of 68.4-76.2 pcs., the distance between the cotyledons of 2.2/2.5-2.7/3.0 cm. |
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Method comprises growing in the nutrient medium of symbiotic bacteria of the genus Xenorhabdus, isolated from nematodes of the genus Steinernema feltiae protense. The culture of these symbiotic bacteria of the genus Xenorhabdus is mixed with the protective medium containing lactose, polyglucin, thiourea, ascorbic acid, dextrin and water in a ratio of 1:1-2. Freezing is carried out at -40°C followed by freeze-drying to a residual humidity of 3-5%. |
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Invention relates to agriculture, in particular dairy cattle breeding. The method consists in the fact that on the 30th day after the first calving the size and density of the ovaries is determined in cows using rectal palpation (or other method), and the animals with atrophy of the right ovary are culled from the herd. |
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Method of assessment of level of morphofunctional development of newborn calves At the age of 1-3 days in the blood of calves the content of foetal haemoglobin, calcium and magnesium is determined. The ratio of calcium and magnesium is calculated. The clinically healthy state and the normal level of development of newborn calves is proved by the level of ratio of calcium/magnesium which is greater or equal to 2.9 and the content of foetal haemoglobin is greater than 60% of the total haemoglobin. The morphofunctional immaturity is proved by the level of the ratio of calcium/magnesium which is less than 2.9 and the content of foetal haemoglobin is less or equal to 60%. Informativeness of the proposed indicators is maintained up to 3 days age, but it is recommended to carry out the assessment of the level of development of calves at the age of 1 day, which enables to carry out more quickly the necessary measures aimed at correcting their physiological state and the prevention of possible diseases. The method enables to assess the muscle metabolism on the ratio of calcium and magnesium, and the usefulness of development of the mechanisms of the haemoglobin synthesis on the level of foetal haemoglobin, changes that occur in the majority of cases of disturbance of prenatal development and/or during birth. |
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Method of breeding coccinellidae harmonia axyridis hall Invention relates to biotechnology, in particular to methods of breeding beneficial insects. The method comprises preparing the nutrient medium comprising soy flour, sucrose, milk powder, palm kernel oil, dry aphides, Wesson salt, dry brewer's yeast, tocopherol, ascorbic acid, agar-agar, vitamins B1, B6, B12, metaben, inositol, and distilled water at a predetermined ratio and growing coccinellidae Harmonia axyridis Hall. at a temperature of 23-26°C in the prepared medium, applied to pieces of sterile polyethylene film. At that the change of feed is carried out daily. |
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Method of stimulating embryogenesis of laying hens Method comprises a single treatment of hatching eggs before laying into the incubator from sprayer with the aerosol of 0.01% sodium salt solution of lipoic acid, which is preliminary dissolved and mixed in distilled water at a temperature of 18-22°C. The solution is applied to the surface of egg shells 3-4 hours prior to their incubation. |
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Regulation of productive characteristics in birds Invention relates to field of biotechnology. Method includes introduction of RNA molecule into a bird egg. Introduced RNA molecule contains double-stranded region and results in reduction of the level of molecule of RNA and/or protein, included into determination of sex in birds, in the egg. Invention can be used in poultry breeding. |
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Method of gathering herd of cows of same breed under conditions of one climatic zone Invention relates to the field of animal husbandry, in particular to a method of gathering the herd of cows. The method of gathering the herd of cows of the same breed under conditions of one climatic zone is characterised by the fact that the animals are first sampled with the duration of service period of 60-100 days and the model of the first approximation is formed from them. Then the animals of model of the first approximation are sampled by age of first insemination which constitutes 14.5-22 months, and the model of second approximation is formed from them. After that from the animals of model of the second approximation the animals are sampled according to the duration of the first pregnancy, which is 270-285 days, and the model of the third approximation is formed from them. From the animals of the model of the third approximation the animals are sampled according to the age at first calving, which is 23.5-31.5 months, and a herd of cows is formed from them. |
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Invention relates to biotechnology. Transgenesis is carried out by conventional artificial insemination of chickens by mediated DNA transfer using human monoclonal antibodies associated on rooster spermatozoa. Detected human monoclonal anti-transferrin receptor antibodies are used. The target recombinant protein is expressed by cells of transgenic chickens in the blood serum and in egg white. The invention can also be used to produce complex recombinant proteins needed in medicine and veterinary. |
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Method of production of nutritional medium for cultivation of coccinellidae harmonia axyridis hall Method comprises metering and mixing in a predetermined sequence of soybean flour, sucrose, dry milk, palm kernel oil, dry greenbug, Wesson salt, dry brewing yeast, tocopherol, ascorbic acid, agar-agar, vitamins B1, B6, B12, metaben, inositol and distilled water. All components are taken in certain proportions. |
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Method of increasing meat productivity of pigs by selection using genetic marker Invention relates to the field of agriculture. The method comprises selection of pigs using a genetic marker. At that the selection and choosing of rearing stock is carried out at achievement of live weight of 90-100 kg in the degree of concentration of free lysine in the muscle tissue of longissimus muscle of back (Musculus Longissimus Dorsi). The animals with the level of lysine concentration of 1.36 mmol/kg of fresh weight and more are left in a breed. |
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Method of increasing adaptive status of bee family Invention relates to livestock husbandry, in particular, to beekeeping for increasing the adaptive status of the bee family. The microdoses of biologically active substance are administered in the body of bees with water or an aqueous solution of carbohydrates. Dihydroquercetin is used as the biologically active substance. |
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Method of stimulation of egg hens embryonic development Invention relates to the field of agriculture and, in particular, to the poultry industry. The method is characterized in that on the surface of the shell of poultry eggs 3-4 hours prior to the incubation the aqueous solution of succinic acid and glycine in a concentration of 0.01% is applied from the sprayer. When transferred into breaking the eggshell on the 19th day of incubation, the 0.5% solution of succinic acid is applied, based on 2.5-2.7 ml per 120-150 eggs at the solution temperature of 20-22°C. |
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Method of feeding vermiculture with nitrogen and device for its realisation Group of inventions relates to biotechnology. Group of inventions is claimed: device for obtaining and realisation of vermiculture feeding on the basis of nitric acid, as well as method of vermiculture feeding with nitrogen by claimed device. Device represents dielectric tube (1) of alternating section with placed in its front part axial ventilator (2), straightening grid (3), evenly distributing air flow, and high-voltage electrodes (4), which create voltage 18-25 kV with frequency 10-200 Hz. Sprayer (5) of pulveriser type with water reserve (6) outside dielectric tube (1) is installed in converging part of tube (1). |
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Method for assessing stress resistance in pigs Invention refers to veterinary science and agriculture. The method provides pig's blood analysis for the following values: malondialdehyde MDA, antioxidant activity AOA, total oxidative activity of blood plasma TOA, catalase activity CT, superoxide dismutase SOD. The two-month pigs having MDA 6.02±0.13 absorption unit/ml and less, AOA 60.86±0.31% and more, TOA 71.64±0.16% and less, CT 57.30±1.05 absorption unit/ml min and more, SOD 14.57±0.44 absorption unit/ml and more are considered to be stress-resistant PSS "-"; the values of MDA 7.63±0.07 absorption unit/ml and more, AOA 50.22±0.42% and less, TOA 79.21±0.83% and more, CT 47.16±1.08 absorption unit/ml min and less, SOD 10.49±0.49 absorption unit/ml and less enables stating the pigs as stress-sensitive PSS "+". The six-month pigs having MDA 7.37±0.17 absorption unit/ml and less, AOA 61.93±0.25% and more, TOA 41.86±0.19% and less, CT 76.68±0.74 absorption unit/ml min and more, SOD 22.80±0.68 absorption unit/ml and more are considered to be stress-resistant PSS "-"; the values of MDA 8.05±0.09 absorption unit/ml and more, AOA 59.72±0.17% and less, TOA 46.21±0.09% and more, CT 71.76±0.51 absorption unit/ml min and less, SOD 19.91±0.59 absorption unit/ml and less enables stating the pigs as stress-sensitive PSS "+". |
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Method of early assessment of laying hens in breeding Invention relates to agriculture, particularly the breeding of poultry. The method of early assessment of laying hens comprises selection of eggs by weight, shape index and shell defects before loading for incubation. At that the embryos are additionally evaluated by the intensity of the embryonic development in the period of incubation by determining on the 18-19 hour of incubation of the diameter of the embryo and determining at the 63-64 hour of incubation of the diameter of the yolk sac vascular field. At that the embryos are selected which diameter of the yolk sac vascular field is not less than 20 mm, and the difference between the diameters of vascular field and the embryo is in a range of 10-15 mm. The method enables to improve hatchability, safety of chickens up to 16 weeks of age, egg-laying capacity of hens for 45 weeks of life, and to accelerate the onset of puberty of hens at 4.0-5.0 days. |
Another patent 2551054.
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