Method for prediction of dysthyroidism |
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IPC classes for russian patent Method for prediction of dysthyroidism (RU 2446401):
Method of predicting development of cerebral metastases in case of lung cancer in women / 2439579
In women in tissue of lung malignant tumour after radical surgery in pulmonectomy volume and in intact lung tissue, obtained from ablated from lung section of the same lung, level of progesterone is determined. If it reduces in tumour in 5 times and more relative the level of progesterone in intact lung tissue, development of cerebral lung cancer metastases in women within the term from 3 to 8 months is predicted.
Method of diagnosing androgenic deficiency / 2439578
In blood serum bioavailable testosterone fraction bound to albumin is determined. If its value is lower than 185.7 pg/ml, disease is diagnosed.
 Method of predicting survival potential of patients with malignant gliomas / 2439577
In patient's urine content of 6-sulfatoximelatonin is determined. If determined content of 6-sulfatoximelatonin after operation before complex treatment equals 292.7 nmol/ml survival to 2.3±0.5 months is predicted, if 22.4 nmol/ml is determined, survival for more than 12 months is predicted. If after chemical therapy determined content of 6-sulfatoximelatonin equals 268.2 nmll/ml, predicted survival time equals to 2.1±0.6 months, if content is 32.8 nmol/ml - more than 12 months. If after a month after treatment determined content of 6-sulfatoximelatonin equals 479.3 nmol/ml predicted is survival for 1.2±0.5 months, in case of 37.4 nmol/ml - survival for more than 12 months.
Method for prediction of cervical cancer metastases / 2436102
In 10-14 days following a surgical removal of a tumour in a patient, daily urine is analysed for sex hormones: oestradiol and pregnandiol. Then, they are related to each other. If the value exceeds 0.8, the onset of metastases or a recurrent disease for the following one and a half or two years is predicted. If the relation is less than 0.8, a metastases-free and recurrence-free period for more than 8-10 years is predicted.
Method of predicting state of fertility in women of reproductive age with uterus myoma / 2433411
At lutein phase of menstrual cycle concentrations of 2-hydroxyestrone 16α - hydroxyestrone in urine, concentration of progesterone in blood serum are determined. Then, canonical value is calculated by formula: K=4.23-0.04×A+0.0016×B-0.18×C, where A is concentration of progesterone in blood serum on 20-21 days of menstrual cycle (lutein phase) (nmol/l), B - concentration of 2-hydroxyestrone in urine (nmol/l), C - concentration of 16α - hydroxyestrone in urine (nmol/l). If K is higher than -0.175, infertility is predicted, if K is lower than -0.175 - safe fertility.
Method of predicting pregnancy in programme of extracorporal fertilisation and transfer of embryos in standard long protocol of superovulation stimulation / 2430379
Before programme of ECF and TE in standard long protocol of superovulation stimulation in patient analysis of hormonal status is performed - content of progesterone receptors in mononuclear fraction of peripheral blood cells is determined. Content of progesterone receptors in mononuclear fraction of peripheral blood cells is determined in the middle of luteal phase of patient's menstrual cycle, before administration of gonadotropin-releasing hormone agonist when carrying out treatment in accordance with standard long protocol of superovulation stimulation. If value is more than 700 progesterone receptors per cell, pregnancy as a result of programme of ECF and TE in standard long protocol of superovulation stimulation is predicted.
Method for prediction of clinical effectiveness of metformin and/or weight-loss therapy in patients with polycystic ovarian syndrome / 2427842
Patient is examined for an anti-Mueller hormone level. If its value is increased by 1/3 and less from an upper limit of normal, a positive menstrual response to treatment is predicted.
Method of predicting risk of thyroid gland diseases in women of perimenopausal age / 2421127
Invention relates to field of medicine, namely to endocrinology. The following data are determined: cause of menopause, application of estrogen preparations, value of index of body weight and thyroid gland volume, concentration of antibodies to thyroid peroxidase and thyrotropic hormone. On the basis of obtained data prognostic coefficient which makes it possible to predict presence or absence of risk of developing thyroid gland diseases in the nearest five years is calculated.
 Method for prediction of developing metastases in patietns suffering rectal cancer / 2420742
Tissue samples of an tumour and perifocal regions are recovered from the preparation removed intraoperatively. They are analysed for the contents of prolactin and testosterone by radioimmunoassay. If the prolactin concentration is 692.0 ng/g of tissue, and the testosterone concentration is 48.0 ng/g of tissue in the tumour, while the testosterone concentration is 42.0 ng/g of tissue in the perifocal region, developing hepatic metastases are expected in 3 months. If the prolactin concentration is 160-390 ng/g of tissue, and the testosterone concentration is 20-40 ng/g of tissue in the tumour, while the testosterone concentration is 24-26 ng/g of tissue in the perifocal region, developing metastases are expected in 29-43 months.
 Diagnostic technique for function-type oddi's sphincter dyssynergia following cholecystectomy / 2416802
Cholecystokinin level is counted additionally in the patients with post-cholecystectomy syndrome. In case the concentration is below 0.5 ng/ml, and while observing no organic pathology of hepatopancreatoduodenal organs as demonstrated by instrument methods, function-type Oddi's sphincter dyssynergia is diagnosed.
 Method for prediction of aggravated bronchial asthma duration / 2446400
Patient's blood plasma is examined for a fractalkine level by ELISA. That is followed by calculating the aggravated bronchial asthma duration (ng/ml) by formula derived by multiple regression analysis: T=B1+B2×Fractalkine, wherein T is the aggravated bronchial asthma duration (days), B1 is a standartised Beta coefficient equal to 8.022120, B2 is a standartised Beta coefficient equal to 6.625970, Fractalkine is the fractalkine level.
 Method for prediction of metastases in patients with stomach cancer / 2445632
Patients suffering stomach cancer are examined for a marker of proliferative activity Ki-67 and markers of apoptosis p53 and bcl-2. That is combined with immunohistological analysis to determine the absolute values in signet ring cell carcinoma: p53 - 10%, bcl-2 -10%, Ki-67 - 12%; in carcinoma simplex: p53 - 13%, bcl-2 11%, Ki-67 - 9%; in adenocarcinoma: p53 - 10%, bcl-2 - 9%, Ki-67 - 12% enabling to predict the onset of metastases in lymph nodes. The values p53 - 6%, bcl-2 -5%, Ki-67 - 10% provide to predict the absence of metastatic lesions in lymph nodes.
 Method for evaluating bacterial lipopolysaccharide antigens in soil / 2445631
Substance of the invention implies producing lipopolysaccharide antibodies, making a soil suspension followed by sorption of bacterial cells and lipopolysaccharides consisting of microsettlement of the produced suspension, conducting the ELISA assay of the microsettlement with the lipopolysaccharide antibodies recovered from blood serum of rabbits immunised by whole bacterial cells Azospirillum treated with 2% glutaric aldehyde, and representing immunoglobulin G, with measuring optical density of the ELISA products, comparing the measured optical densities with the reference values with the presence of the bacterial lipopolysaccharide antigens is stated by a deviation of the measured values from the reference by a value of 10-15% and more.
 Method for prediction of risk of decreasing body resistance to acute respiratory diseases in children between ages of 3 to 7 by immunological criteria / 2445630
Child's peripheral blood is examined to evaluate the percentage of lymphocytes and the concentration of immunoglobulin M by turbidimetric method in g/l. If the concentration of immunoglobulin M is higher than 1.125 g/l, while the percentage of lymphocytes is higher than 47% (oppositely directed deviances), then a child is referred to a risk group with decreased body resistance to acute respiratory diseases.
 Binding agent of pathological prion protein and method for detecting pathological prion protein / 2444735
What is offered is a binding agent of a pathogenic isoform of a prion protein containing lactoferrin. There are offered a method for detecting and a method for recovering the pathogenic isoform of the prion protein involving the stage of sample contacting with the binding agent of the pathogenic isoform of the prion protein and the stage of binded component separation from the binding agent of the pathogenic isoform of the prion protein contacting with the sample. The method for detecting additionally involves the stage of detection of the pathogenic isoform of the prion protein found in the component of the pathogenic isoform of the prion protein separated from the binding agent.
Method for prediction of early infectious miscarriage / 2444734
For the purpose of prediction of an early infectious miscarriage, blood serum of pregnant woman on her 1 trimester is examined for a level of a soluble form of sFlt-1 receptor. If its value is 0.389 ng/ml or lower, the miscarriage is predicted.
Method for prediction of clinical outcome of penetrating keratoplasty / 2444733
Method for corneal engraftment prediction involving the ELISA examination of lachrymal fluid for the preoperative content of interleukine-1-beta (IL-1β), differing by the fact that the content of IL-10 in lachrymal fluid is additionally evaluated that is followed by making the IL-1β to IL-10 relation, and if the derived value falls within 0.15-0.20, a favourable clinical outcome of the keratoplasty achieving a clear corneal engraftment, while the value of 0.35 or higher shows an unfavourable clinical outcome accompanied by opacity.
Method for prediction of developing complications in patients with community-acquired pneumonia / 2444017
Blood serum is examined for procalcitonin (PCT, pg/ml), tumour necrosis factor-α (TNF-α pg/ml), and C-reactive protein (CRP, mg/l) values. These values are used to derive the value D: D=+12.444*PCT+0.13*CRP+2.589* TNF-α. If the value D exceeds 54.25, the complicated clinical course of community-acquired pneumonia is predicted.
 Method for detection of sugar chains with glcnac synthesized by gnt-v / 2442989
FIELD: biotechnology. SUBSTANCE: methods for determination, purification and filtration of sugar chains containing GlcNAc synthesized by GnT-V using at least one type of GlcNAc-specific lectins with affinity to sugar chains with GlcNAc synthesized by GnT-V, that are chosen out of the group including GSL-II and PHA-L and at least one type of GlcNAc-specific lectins with no affinity to sugar chains with GlcNAc synthesized by GnT-V, that are chosen out of the group including BLL and ABA and are used in combination as a medium for determination of sugar chains with GlcNAc synthesized by GnT-V or in combination for contact with sugar chain sample. One of the group of the inventions is a set including two types of GlcNAc-specific lectins. EFFECT: development of precise method for determination of sugar chains with GlcNAc synthesized by GnT-V. 4 cl, 1 ex, 1 tbl, 5 dwg
Means of nonspecific serodiagnosis of syphilis seronegative forms / 2442536
FIELD: medicine. SUBSTANCE: invention refers to venereology and can be used for nonspecific serodiagnosis of syphilis seronegative forms. This requires the identification of nonspecific (anticardiolipin) antibodies by means of nonspecific serological reaction to syphilis. In case of no response to serological reactions the highly specific antigen of cultural pale treponemas are inserted into the body beforehead. Then after a certain period of time set for the syphilitic infection activation the anticardiolipin antibodies are identified. If the anticardiolipin antibodies are identified syphilis is diagnozed. EFFECT: means provides the improvement of the accuracy of nonspecific serodiagnosis of syphilis seronegative forms due to the activation of the syphilis process as a result of inserted highly specific antibody of cultural pale treponemas which allows changing the course of seronegative syphilis that is characterized by no immune reply to the syphilis activator to the seropositive form that is characterized by the immune reply to the activator. 1 dwg  Method for predicting the character of bacterial keratitis flow / 2245553
In lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula:
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FIELD: medicine. SUBSTANCE: method involves woman's pre-delivery blood serum examination for nitrogen oxide and relaxin that is followed by calculation of the NO/relaxin relation. If the relation is 3.6 and less, dysthyroidism is predicted. EFFECT: use of the method provides high-accuracy preclinical prediction of dysthyroidism that enables well-timed optimisation of management and treatment in the patients suffering given pathology. 3 ex
The invention relates to medicine, namely to obstetrics, and can be used to predict discoordination labor. Many aspects of early diagnosis and prediction of pathology in childbirth, and in particular, discoordination generic activities requires new approaches using technologies of molecular biology and medicine. Pathology contractile activity of the uterus leads to an increase in the duration of delivery, number of surgical interventions, the frequency of obstetric injury, pathologic hemorrhage, intrauterine asphyxia of the fetus. Dramatically increases perinatal mortality and mestorozhdenii. It is known that for discoordination labor activity is characterized by the presence of abnormal contractions that differs from the physiological not the absolute voltage value of the myometrium during its decline as a violation of the procedure for changing the amounts of the various divisions of the uterus (Art. Active management of labour. // Special literature, St. Petersburg., 1999, 667 S.). Direct inducer of contraction of the smooth muscle cells of the myometrium are calcium ions (CA2+quickly coming from the extracellular environment to the cytoplasm through the opening in plasmalemma CA2+-channels. The action of the muscles that are specific regulators contra is tilen activity of the uterus, by maintaining physiological levels of calcium ions in the cells of the myometrium. Violation of their products leads to an imbalance of the ratio of intra - and extracellular level of CA2+accompanied discoordinating reduction cells of the myometrium (Vierbuchen, Lierelease "Premature birth and new strategies for their correction: a literature review". Bulletin SB RAMS, №3 (131), 2008). For the prevention of discoordination labor activity is important for timely detection of this pathology. There are methods of diagnosing abnormalities of labor activity using hysterography: external and internal (Art. Active management of labour. // Special literature, St. Petersburg., 1999, S. 667). A common shortcoming of these methods is that they diagnose the abnormality of labor activity at the stages of its manifestation. While the disadvantages of internal hysterography should be considered at high risk of developing complications from the mother and the fetus (intrauterine infection, acute asphyxia, detachment of normally situated placenta, as well as the need in interpreting results involving complex, bulky programs using a computer). There is a method of predicting pregnancy outcome in threatening childbirth (RF patent No. 2132069 published 20.06.1999)by defining p is referecnce blood content CD16 and CD3 and calculate their ratio, largest of which predict risk of premature birth. The disadvantages of this method should include the fact that it can not predict the nature of labor, and the possibility of premature birth. A known method for predicting anomalies labor before childbirth (RF patent No. 2134418 published 10.08.1999) forecasting anomalies of labor activity by ultrasonic method, examining the tissue of the uterus on the border with the lower segment and identifying hyperechoic line on the background hypoechoic myometrium predict this pathology. The disadvantages of this method include the fact that this method does not allow us to predict discoordination labor activity, and therefore cannot carry out targeted therapy. From the practice of medicine is known a method for predicting anomalies labor activity (patent RF №2229722 published 27.05.2004), including the determination of enzyme activity in peripheral blood in women at 38-39 weeks of gestation. The disadvantages of this method should include significant difficulties in obtaining the true value of the activity of Na, K-ATPase due to the complexity of the conditions during the implementation of this study, resulting in the observed high percentage of false-positive results. Being the major disadvantage of the method can be considered as the absence of a differential nature of the violation of labor. There is a method of predicting discoordination labor in pregnant women risk group (patent RF №2235499 published 10.09.2004)by registering oscillations of heart rate in the low frequencies (LF)and high frequency (HF) and their values shall prognosis of this disease. This method is designed only for pregnant women at risk, which limits its use in other conditions. A known method for predicting violations of labor activity (patent RF №2268465 published 20.01.2006) by microscopy obtained serum samples dried in the form of a drop on a glass slide at a temperature of 18-25°C for 18-24 hours. In the presence of extremely low levels of structureborne and the absence of a radially symmetric pattern of facies, predict a violation of the generic activities that require rapid completion of the delivery. A condition for the realization of the developed method of forecasting is the beginning of the run it for 3-5 days before giving birth. Moreover, in the case of development over this period of preterm birth, it cannot be used. Other disadvantages of this method include the fact that it is impossible to predict the type of violation of labor. A known method for predicting anomalies labor, fixed (patent RF №2296993 published 10.04.207), which consists in determining osmoresistens erythrocytes in the presence of blocker-1 (1-isopropylamino)-3(1-naphthalenyl-oxy)-2-propanol hydrochloride and under certain its values predict anomalies of labor activity in the preliminary or first stage of labor. The disadvantage, of course, is that the data on which it is possible to predict anomalies labor activity obtained in the preliminary or first stage of labor, and this reduces the possibility of the timely appointment of therapy. In addition, this method cannot be differentiated to predict the weakness of the labour forces or their discoordination. The prototype of the present invention is elected as a method for predicting discoordination generic activities described in the copyright certificate №16483446 (bull. 1991, No. 18, p.17). The essence of the prototype method is that in the blood of the cervix to determine the activity of adenozindezaminazy and increase the norm predict discoordination. The disadvantages of this method include: Forecasting discoordination generic activities carried out during the period of childbirth, which significantly reduces time opportunity for preventive measures. These drawbacks are eliminated in the invention. The objective of the claimed invention - development method is, allowing even at the preclinical stage to predict with high accuracy discoordination labor. The problem is solved as follows: at the end of pregnancy at 38-40 weeks in serum by ELISA to determine the content of metabolites of nitric oxide (NO2-and NO3-)equivalent to the level of nitric oxide, and relaxin, when the ratio of the level of nitric oxide (NO) to the level of relaxin equal to 3.6 and below, predict discoordination labor. The technical result obtained during use of the invention is to improve the prediction accuracy of discoordination labor activity in the antenatal assessment in the serum ratios of nitric oxide and relaxing, which enables it to form a high risk group. Contractile activity of the myometrium is coordinated by the combined action of various bioactive substances, acting as its uterotonic and relaxants. In the processes of spontaneous labor activation are regulatory mechanisms, an important place among whom are the connections that provide the reaction to the relaxation of the myometrium during labor. The latter include nitric oxide and relaxing (Min G. et al. Eridence that endogenous relaxin promotes growth of the vagina and uterus during pregnancyin gifts. Endocrinology, 1997, 138, 560-565; Bgerman, Ividorami, Mguni. Structural and functional basis of coordination of the contractile activity of the myometrium (the vascular system of the uterus). Grew up with. lead, akush.-gin., 2003, 5, 21-22). Nitric oxide (NO)as an endogenous inhibitor of the reduction of the myometrium, acts as a paracrine regulator, indicating its important role in labor activity. Thus relaxin by binding to receptors in the myometrium increases the level of camp, while the nitric oxide increases cGMP, which results in the opening of CA2+channels, due to the corresponding concentration of CA2+on the physiological background of its revenues in the cells of the myometrium (Vierbuchen, Lierelease "Premature birth and new strategies for their correction: a literature review". Bulletin SB RAMS, No. 3 (131), 2008: S. 38-44). The imbalance of these regulatory processes causes the change of transport Sa2+through slotted contacts myocytes, which leads to disruption of coordinated contractions of the myometrium. Changes during pregnancy products relaxin and absence of nitric oxide leads to the disorganization of the contractile function of the myometrium, which in turn is implemented in discoordination labor, because, obviously, at physiological for the generic act equally necessary soo the appropriate level of nitric oxide, and relaxing. Detailed description of the method and examples of its clinical application. Pregnant women before labor activity in the period 38-40 weeks perform taking blood from the cubital vein with subsequent obtain serum, which determine the content of metabolites of nitric oxide (NO2-and NO3-and relaxin. Calculate the ratio and amount equal to 3.6 and below forecast in this patient, discoordination labor. Endogenous levels of nitric oxide, equivalent to the level of metabolites of nitric oxide (NO2-and NO3-)specify in 0.1 ml of serum using the classical reaction Griss (L.Green et al., 1982) recruitment firm R&D (USA) in accordance with the Protocol of the manufacturer. The optical density of the color of the solution obtained after the reaction, register on the photocolorimeter "Victor multilabe counter - 1420 (Finland). Determination of the level of relaxin in serum perform enzyme-linked immunosorbent assay using kits (R&D Systems, Inc., USA) on the device "Immunolite". The performance of the claimed method the following examples. Example 1. Pregnant-VA NV, 31. Case history No. 5292/843 from 27.12. 2009 Diagnosis: a Birth I, the term 39-40 weeks. Age perforada. 28.12.200 year after informed consent taken blood from the cubital vein for determination of metabolites of nitric oxide (NO 2-, NO3-)equivalent to the level of nitric oxide (NO), and relaxin. The content of nitric oxide in the blood serum was 1811 PG/ml, and relaxin 488 PG/ml the Ratio of the nitric oxide/relaxin was reached 3.7. According to our method, is obtained allowed us to predict the optimal course of childbirth. 29.12.2009 year, the patient began labor activity. Data delivery was uneventful and ended through the birth canal alive full-term, healthy baby. Example 2. Pregnant To the Islands ETC., 29 years. Case history No. 5585/818 from 6.02.2009, Diagnosis: a Birth I, the period of 40 weeks online. Dystocia cervix. Intrauterine hypoxia. Age perforada. Large fruit. 7.02.2009 year after informed consent taken blood from the cubital vein for determination of metabolites of nitric oxide (NO2-, NO3-)equivalent to the level of nitric oxide (N0), and relaxin. The content of nitric oxide in the blood serum was 1650 PG/ml, and relaxin 458 PG/ml the Ratio of the nitric oxide/relaxin was equal to 3.6. This ratio can predict discoordination labor. 8.02.2009 year women began labor activity. Childbirth has been accepted way; was carried out corticotropinby monitoring. Effective the guard force and frequency of contractions, as well as the disclosure of the cervix was assessed through monitoring. Data readings used diagnostic equipment and the nature of clinical symptoms (dystocia cervix, hypertonicity of the uterus) testified to discoordination labor. Childbirth is completed by an emergency caesarean section. He was born full-term, live baby. This clinical example confirms the possibility of predicting discoordination labor. Example 3. Pregnant B-VA, I.I., 32 years. History of births No. 3421/616 from 16.09.2010. Was admitted to hospital for birth 17.09.2010, Diagnosis: a Birth I, the period of 40 weeks online. Dystocia cervical cancer, once the tight entanglement of the umbilical cord of the neck of the fetus. Late gestosis E, hypothyroidism Art. I, chronic autoimmune thyroiditis. Colpitis. After informed consent was taken blood from the cubital vein for determination of metabolites of nitric oxide, equivalent to the level of nitric oxide (NO), and relaxin. The concentration of metabolites of nitric oxide (NO2-NO3-) in the serum was 1600 PG/ml, and relaxin 513 PG/ml Ratio NO/relaxin corresponded to 3.1. This ratio can predict discoordination labor. 18.09.2010 mothers at 39-40 weeks began labor activity. Childbirth led the under monitor cardiocentro. Power efficiency and regularity of contractions and cervical dilatation was assessed through monitoring. Was diagnosed discoordinated labor, and fetal - acute distress. Performed a caesarean section with a favorable outcome. Born live, full-term baby. This clinical example confirms the possibility of early detection of discoordination generic activities and effective obstetric tactics. Diagnostic specificity and accuracy of the method is equal to 100% (no false-positive and false-negative results). By the present method were examined in 59 women, among whom 31 pregnant diagnosed physiological childbirth. The latter value NO/relaxin in serum was equal to 3.7 and above. With 28 women indicator was equal to 3.6 and below, combined with discoordinating labor. The above confirms the prognostic value of the claimed method. Thus, the inventive method is specific and accurate than known. 1. The method is specific, as it allows the ratio of substances (and NO relaxin), which is the specific muscles that control in smooth muscle cells of the myometrium level of calcium ions, providing the contractile activity of the uterus, prognozirovaniya labor. 2. It has high precision. 3. Clinical application of the method will allow the timely implementation of obstetric tactics. In clinical practice, this will allow us to predict discoordination generic activities to optimize the management and treatment of patients with this pathology. The inventive method of predicting discoordination labor activity tested at a sufficient volume of clinical material and can be recommended for use in obstetric hospitals. A method for predicting discoordination labor activity by biochemical analysis of blood, characterized in that in the antenatal period in the serum of pregnant immunoassay method to determine the content of metabolites of nitric oxide and relaxing with subsequent calculation of their ratio and amount equal to 3.6 and below, predict discoordination labor.
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