Method of predicting pregnancy in programme of extracorporal fertilisation and transfer of embryos in standard long protocol of superovulation stimulation |
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IPC classes for russian patent Method of predicting pregnancy in programme of extracorporal fertilisation and transfer of embryos in standard long protocol of superovulation stimulation (RU 2430379):
Method for prediction of clinical effectiveness of metformin and/or weight-loss therapy in patients with polycystic ovarian syndrome / 2427842
Patient is examined for an anti-Mueller hormone level. If its value is increased by 1/3 and less from an upper limit of normal, a positive menstrual response to treatment is predicted.
Method of predicting risk of thyroid gland diseases in women of perimenopausal age / 2421127
Invention relates to field of medicine, namely to endocrinology. The following data are determined: cause of menopause, application of estrogen preparations, value of index of body weight and thyroid gland volume, concentration of antibodies to thyroid peroxidase and thyrotropic hormone. On the basis of obtained data prognostic coefficient which makes it possible to predict presence or absence of risk of developing thyroid gland diseases in the nearest five years is calculated.
 Method for prediction of developing metastases in patietns suffering rectal cancer / 2420742
Tissue samples of an tumour and perifocal regions are recovered from the preparation removed intraoperatively. They are analysed for the contents of prolactin and testosterone by radioimmunoassay. If the prolactin concentration is 692.0 ng/g of tissue, and the testosterone concentration is 48.0 ng/g of tissue in the tumour, while the testosterone concentration is 42.0 ng/g of tissue in the perifocal region, developing hepatic metastases are expected in 3 months. If the prolactin concentration is 160-390 ng/g of tissue, and the testosterone concentration is 20-40 ng/g of tissue in the tumour, while the testosterone concentration is 24-26 ng/g of tissue in the perifocal region, developing metastases are expected in 29-43 months.
 Diagnostic technique for function-type oddi's sphincter dyssynergia following cholecystectomy / 2416802
Cholecystokinin level is counted additionally in the patients with post-cholecystectomy syndrome. In case the concentration is below 0.5 ng/ml, and while observing no organic pathology of hepatopancreatoduodenal organs as demonstrated by instrument methods, function-type Oddi's sphincter dyssynergia is diagnosed.
Method of predicting duration of recurrence-free period in radically treated patients with mammary gland cancer / 2413231
Content of sex hormones -estradiol and progesterone - in blood is determined. After that coefficient of ratio of estradiol concentration to progesterone concentration is calculated. If values of coefficient are within the range from 10 to 221, duration of recurrence-free period from 5 to 13 years is predicted, and if values of coefficient are within the range 367-1295 recurrence-free period for 28-30 months is predicted.
Method of predicting of process activation in patients with hodgkin's lymphoma / 2405454
Invention relates to medicine, namely to biochemical investigations in oncology, and can be used in determination of activation or stabilisation of pathologic process in patients with primary-resistant Hodgkin's lymphoma. In patients with primary-resistant Hodgkin's lymphoma at stages of treatment, by radioimmune method determined is content in blood of thyroid gland hormones - triiodothyronine and thyroxine, and adrenocortical hormone - cortisol, their concentration is summed up and in case of total level of hormones is within 278.2 nmole/l - 0 333.0 nmole/l conclusion about activation of malignant process is made, if total level of said hormones is 432.4 nmole/l - 833.2 nmole/l conclusion about absence of process activation is made.
Diagnostic technique for severity level of varicocele and its recuring / 2403871
Invention refers to medicine, namely to urology and vascular surgery. The sex hormones level in an involved vein is considered to be a criterion of the severity level of varicocele. There are evaluated the following sex hormones: testosterone, estradiol, progesterone simultaneously in the pampiniform plexus veins of a left ovary and in the peripheral vein of a bend of elbow, with determining the ratio of the sex hormones level in a healthy vein of the bend of elbow to the varicocele-involved pampiniform plexus vein. If said ratio of the sex hormones level is 1:10 to 1:50, the first degree of varicocele is diagnosed. Recurrent varicocele and the second degree of varicocele is diagnosed by the ratio of the sex hormones level in the healthy and involved veins 1:50 to 1:100 times (i.e. hormones concentration in a varicose vein is increased in tens times). If observing the ratio of the sex hormones level in the healthy vein and the involved vein of the pampiniform plexus veins of the left ovary increasing more than in 1:100, the third degree of varicocele is diagnosed. Surgical intervention is indicated if the ratio of the sex hormones level is diagnosed more than 1:50.
 Method of bone resorption to remodelling analysis / 2403870
Invention refers to medicine, namely to biochemistry, surgery and dentistry. The bone resorption to remodeling analysis is based on the biochemical blood examination. An the blood coefficient K1 is calculated by formula: K1=[(PTH:A)+(TNF-α:B)+(IL-1β:C)]:3, where PTH is the parathyroid hormone concentration (pg/ml) in the patients, A is the same value in healthy individuals, TNF-α is the level of tumor necrosis factor -α (pg/ml) in the patients, B is the same value in healthy individuals, IL-1β is the interleukin-1β concentration (pg/ml) in the patients, C is the same value in healthy individuals to derive the blood coefficient K2 by formula: K2=[(calcitonin: D) + (osteocalcin: E)]:2, where the calcitonin concentration (ng/ml) in the patients, D is the same value in healthy individuals, the osteocalcin level (ng/ml) in the patients, E is the same value in healthy individuals. Then the bone metabolism control coefficient (BMCC) is calculated by formula BMCC=K1:K2, and the higher BMCC than 1.17, the more intensive resorption prevails over remodelling; and the lower BMCC than 0.83, the more intensive remodelling prevails over resorption.
Method for assessment of kidney function in felines by measurement of ghrelin hormone levels / 2403573
Method for assessment of kidney function in felines includes determination of observed level of ghrelin in tissue or biofluid of feline and establishment of direct dependence of observed level of ghrelin on kidney function by comparison of observed level of ghrelin with reference level of ghrelin that characterises normal kidney function. Method for diagnostics of kidney disease in felines includes detection of observed level of ghrelin in tissue or biofluid of feline and comparison of observed ghrelin level with referent level of ghrelin, which characterises normal kidney function, where observed level lower than reference level indicates kidney disease or predisposition to it. Method for detection of kidney disease beginning in felines includes monitoring level of ghrelin in tissue or biofluid of feline for a certain period of time; where beginning is identified, if at any moment of time level of ghrelin decreases compared to initial level that characterises healthy kidney function. Diagnostics set includes (a) one or more test materials to detect observed levels of ghrelin in tissue or biofluid of feline; and (b) one or more user-available mediums bearing the following information: (i) reference level of ghrelin that corresponds to a specific feline; and (ii) algorithm of direct dependence of observed ghrelin level relative to reference level on kidney function or reverse dependence of observed ghrelin level relative to reference level on availability of kidney disease or predisposition to kidney disease.
Diagnostic technique for foetal maturity / 2399058
There are determined daily excretion of progesterone metabolite - pregnandiol (Pd), foetal steroids - 16α-hydroxy-ethiocholanolone (16α-OH-Et), 16α-hydroxy-androsterone (16α-OH-An), 16α-hydroxy-dihydroepiandrosterone (16α-OH-DHEA) and oestradiol (Oe) in pregnant women on their 37-42 weeks of pregnancy by capillary gas chromatography. A mature foetus is indicated by the following indices: daily Pd excretion is 150.4-348.3 mcmol/24 hour, 16α-OH-Et - 7.9-16.9 mcmol/24 hour, 16α-OH-An - 6.4-12.6 mkmol/24 hour, 16α-OH-DHEA - 15.4-26.1 mcmol/24 hour, Oe - 24.0-38.7 mcmol/24 hour, while an immature foetus show the indices as follows: daily Pd excretion 80.1-150.2 mcmol/24 hour, 16α-OH-Et - 5.8-7.8 mcmol/24 hour, 16α-OH-An - 4.6-6.3 mcmol/24 hour, 16α-OH-DHEA - 10.2-15.3 mcmol/24 hour, and Oe - 14.5-15.6 mcmol/24 hour, and in a overmature foetus, daily Pd excretion, 16α-OH-Et, 16α-OH-An, 16α-OH-DHEA and Oe are considerably lowered and are less than 50.0 mcmol/24 hour, less than 12.0 mcmol/24 hour, less than 5.8 mcmol/24 hour, less than 4.6 mcmol/24 hour, less than 10.2 mcmol/24 hour, respectively.
Method for differential diagnostics of mammary diseases in men / 2244308
The present innovation deals with biochemical trials: before the onset of therapy in men one should detect blood content of thyroid hormone - free thyroxine - and at its level being 10.3-12.9 pmol/l one should diagnose mammary cancer, at the level of free throxine being 18.7-31.0 pmol/l - one should predict gynecomastia. The method enables to detect the direction of pathological process and carry out due correction of therapy tactics in men with either gynecomastia or mammary cancer.
Method for predicting the delay of intrauterine fetal development / 2246733
The method deals with studying blood serum of pregnant woman to detect the content of insulin-like growth factor (IGF) and vascular-endothelial growth factor (VEGF) and calculate the coefficient of their ratio: at its value being equal to 28.5 and lower it is possible to diagnose the delay of fetal development.
Method for differential diagnostic of chronic hepatitis and hepatic cirrhosis / 2254577
Thyroglobulin content is determined in blood serum using enzyme immunoassay. When thyroglobulin level is increased by 2 times and more compared in contrast with normal one chronic hepatitis is diagnosed, and when thyroglobulin level is decreased by 1.5-2.5 times in contrast with normal one hepatic cirrhosis is diagnosed.
Method for evaluating combined schizophrenia treatment based on atypical antipsychotic drugs and rations and electroconvulsive shock therapy / 2256181
Method involves determining dehydroepiandrosterone sulfate concentration in blood serum. Its growth above 30% when compared to the initial one being observed, treatment efficiency is determined as negative.
Method for diagnosing obliterated forms of congenital suprarenal gland cortex hyperplasia / 2261447
Method involves applying high effectiveness liquid chromatography for determining cortisol, cortisone 11-deoxycorticosterone, 11-deoxycortisone concentration in blood and free cortisol and free cortisone excretion with urine. Ratios of F/E and FF/FE are calculated, where F is the cortisol level in blood; E is the cortisone level in blood; FF is the free cortisol excretion with urine and FE is the free cortisone excretion with urine. The cortisol level in blood not exceeding norm and at least two of three signs: F/E ratio reduction by 25% and more, FF/FE ratio reduction by 25% and more, free cortisol excretion with urine being equal to 25% and more, obliterated forms of congenital suprarenal gland cortex hyperplasia is diagnosed. Corticosterone level in blood growing by 50% and higher, 21-hydroxylase defect is considered to be the case. 11-deoxycorticosterone and/or 11-deoxycortisone concentration in blood being et or greater than 50%, 11β-hydroxylase defect is considered to be the case.
 Method for diagnosing atrophic gastritis cases / 2262706
Method involves making pepsinogen 1, gastrin and Helicopter pylori infection marker combinations analysis and making input of the obtained results into data processing means comprising operation system, means for receiving, transmitting and processing data. The mentioned data processing means is usable for comparing the measured concentration value of a substance under study to a threshold value associated to the substance under study and producing information as a response to comparison results and additionally to other entered data. A set and software are used for implementing the method.
Method for predicting relapse of mammary cancer / 2263319
In the course of surveying in menopausal women after complex therapy one should state the development of mammary cancer at decreased ratio of estriol concentration to the sum of estrone and estradiol urinary concentrations from 1.68±0.23 in relapse-free patients up to 0.74±0.12 - in patients living without relapses for less than a year, up to 0.65±0.13 in patients living without relapse from 2 up to 6 years and up to 0.50±0.10 in patients with relapse-free period from 6 to 10 years. The innovation provides pre-clinical detection of mammary cancer relapse.
Method for predicting fetoplacental insufficiency in pregnant women with thyroid diseases / 2263919
Except detecting placental lactogen in blood serum one should study the content of alphafetoprotein. At placental lactogen content being below 75% against the norm and content of alphafetoprotein below 70% against the norm it is possible to conclude upon availability of fetoplacental insufficiency.
Method for predicting cholecystitis and cholelithiasis / 2263920
While diagnosing cholecystitis and cholelithiasis due to ultrasound testing one should additionally study blood plasma and bile to detect there the content of prostaglandins PGE2 and PGF2α. At PGE2/PGF2α ratio in blood plasma being equal to 6 and more, and, also, at decreased level of biliary cholecystokinin-pancreosimin by 38% and more, biliary PGE2 by 59% and more and increased level of biliary prostaglandin PGF2α by 5.9 times and more against the norm one should diagnose chlecystitis and cholelithiasis. The innovation enables to detect the above-mentioned diseases at earlier stage.
Method for predicting powerless labor in pregnant nodular goiter surgically treated on goiter occasion during pregnancy under medical supervision / 2273456
Method involves determining thyroid gland node diameter and thyrotropic hormone by applying ultrasonic examination approach. Histological examination is carried out with conclusion concerning morphologic nature being obtained like nodular colloid proliferating goiter or thyroid gland adenoma. Diagnostic index Σ is calculated from formula Σ=0.49*K1+0.07*K2-0.5*K3+1.76*K4-1.53, where K1 is the thyroid gland node diameter; K2 is the TTH concentration; K3 is the nodular colloid proliferating goiter index equal to 1 or 0; K4 is the thyroid gland adenoma index equal to 1 or 0. Diagnostic index Σ being less than zero, conclusions concerning powerless labor threat is to be drawn.
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FIELD: medicine. SUBSTANCE: before programme of ECF and TE in standard long protocol of superovulation stimulation in patient analysis of hormonal status is performed - content of progesterone receptors in mononuclear fraction of peripheral blood cells is determined. Content of progesterone receptors in mononuclear fraction of peripheral blood cells is determined in the middle of luteal phase of patient's menstrual cycle, before administration of gonadotropin-releasing hormone agonist when carrying out treatment in accordance with standard long protocol of superovulation stimulation. If value is more than 700 progesterone receptors per cell, pregnancy as a result of programme of ECF and TE in standard long protocol of superovulation stimulation is predicted. EFFECT: method ensures analysis simplicity, reduction of its cost, increase of prediction reliability due to taking into account of objective index, indirectly characterising patients' endometrium hormonal status, by determination of progesterone receptors level in mononuclear fraction of peripheral blood cells before beginning treatment. 2 ex
The invention relates to medicine, in particular to the gynecologist. Despite being developed for more than 30 years methods of assisted reproductive technology, the effectiveness of even the most effective method of in vitro fertilization and embryo transfer (IVF and embryo transfer) leaves much to be desired: implantation rate is quite low - 25-35% according to the European IVF-Monitoring Program, 2005. Conventional prognostic factors of success of in vitro fertilization and embryo transfer are well known and include: The age of a patient. A large number of works show a sharp decline in the effectiveness of the program every year after 35 years of age (Centers for Disease Control and Prevention, ASRM, 2003). - Follicular ovarian reserve, as measured by the number of antral follicles in the ovaries by ultrasound studies or level antinucleosome hormone. Many studies have shown a decline in the effectiveness of the program in 2-3 times in patients with poor ovarian response to stimulation by gonadotropins (Hendriks DJ, et al., Reproductive biomedicine online 2008 Nov; 17(5):727-36). - Smoking. For smokers require about 2 times more attempts of in vitro fertilization and transfer of embryos to achieve the desired result (Freour T et al., Reproductive Biomedicine Online 2008 Jan; 16(1):96-102). - Obesity. Patients suffering from obesity, for achievement of the pregnancy, on average, require more cycles of in vitro fertilization and embryo transfer (Orvieto R, et al., Reproductive Biomedicine Online 2009 Mar; 18(3):333-6). The non-Smoking patients younger than 35 years, not obese and normal follicular reserve, to predict the results of in vitro fertilization and embryo transfer is also very difficult. In this regard, developed a more sophisticated forecasting methods, which require further research and refinement. Known methods for predicting outcomes of IVF and embryo transfer, comprising determining the level of interleukin 12, 15, 18 and cells-natural killer cells CD56+ endometrial biopsy or swabs from the uterine cavity (Ledee-Bataille et al., Fertility Sterility 2005 Mar; 83(3):598-605), determining the level of integrin αVβ3 in the biopsy of the endometrium (Tei et al., Journal of Assistant Reproduction and Genetics 2003 Jan; 20(1):13-20), the determination of the level of matrix metalloproteinases 2 and 9, interleukin 1 and 10, and gamma interferon in the aspirates of the uterine cavity (Inagaki et al., Human Reproduction 2003 Mar; 18(3):608-15). It was also suggested determining the level of matrix RNA endometrial aromatase P450 in the biopsy of the endometrium (Brosens et al., Human Reproduction 2004 Feb; 19(2):352-6). The disadvantages are invasive, i.e. the removal of a biopsy or flushing of the uterine cavity, as well as the complexity of the interpretation of the results in the analysis of runoff from the uterine cavity. It is also known that receptors estradiol and progesterone in mononuclear FR the functions of peripheral blood cells can be carried out diagnosis of estrogen - and progesteronepositive pathology genitalia (EN 2312354 C1, 10.12.2007). However, information about the value of these parameters to predict pregnancy with in vitro fertilization in the literature are missing. As a prototype we have chosen a method for predicting pregnancy in a long Protocol IVF and embryo transfer by determining the expression of cytokines of T-helper link the peripheral blood lymphocytes of patients (Kwak-Kim JY et al., Increased T helper 1 cytokine responses by circulating T cells are present in women with recurrent pregnancy losses and in infertile women with multiple implantation failures after IVF, Human Reproduction 2003 Apr; 18(4):767-73). The disadvantage of this method is its complexity: the need for a separate determination of the fractions of lymphocytes and their functional activity level expresii their various cytokines and, consequently, its high cost and low accuracy. We were tasked to create an informative non-invasive method for predicting the pregnancy rate in IVF and embryo transfer in the standard long Protocol of stimulation of superovulation. The technical result is to provide easy research, reduce its cost, improve the reliability of the prediction by taking into account an objective indicator that indirectly characterize the hormonal status of the endometrium of patients, by determining the level of progesterone receptors in mononuclear F. the action of peripheral blood cells before treatment. The invention consists in the following. Before IVF and embryo transfer in the standard long Protocol of stimulation of superovulation the patient perform a study of the hormonal status - determine the content of progesterone receptors in mononuclear fraction of peripheral blood cells. The content of progesterone receptors in mononuclear fraction of peripheral blood cells is determined in the mid-luteal phase of the menstrual cycle of the patient, before the appointment of an agonist of gonadotropin-releasing hormone during the treatment according to the standard long Protocol of stimulation of superovulation. When the value of more than 700 of the progesterone receptor on the cell predict the onset of pregnancy as a result of the IVF program and PE in standard long Protocol of stimulation of superovulation. We empirically found that this indicator has been informative predictor of pregnancy in IVF and embryo transfer in the standard long Protocol of stimulation of superovulation. We established indicator indirectly, but objectively evaluate the hormonal status of the endometrium of the patient, which is a determinant of pregnancy. This indicator is likely to predict the willingness of endome is the right of the woman to the implantation of the embryo. The proposed method is simple as it involves only a single metric, and the production of the investigated material (peripheral blood) is not connected with the carrying out invasive procedures. The method is as follows. To determine the content of progesterone receptors in mononuclear fraction of peripheral blood cells can be used the methodology described in the description of the patent (RU 2312354 C1, 10.12.2007). Mononuclear cells peripheral blood allocate the standard method [Boyum A. Separation of leukocytes from blood and bone marrow // Scand. J. Clin. Lab. Investig. - 1968. - Vol.21 - Suppl. 97. p.1-9] from heparinised blood (7-10 IU of heparin/ml) with modifications. Blood diluted with a physiological solution (pH of 7.2) in the ratio 1:2. Diluted saline blood (5 ml) layer on warmed to room temperature ficoll (Pharmacia Fine Chemicals, Sweden, density 1, 077 g/ml, 3 ml centrifuge tube and centrifuged at 400g for 30 min at 4°C [Kheifets L. B., Abalkin, VA separation of the formed elements of human blood. // J. Lab. business, 1973. - 10. - s-581]. The interphase select a sterile glass pipette and the resulting mononuclear cell fraction is washed three times colorless Hanks solution (cell resuspending in a tenfold volume of Hanks solution and centrifuged 5 minutes at 250 g). The last residue raise in 1 ml of media and calculate the camera Goryaeva. After calculating diluted with medium to a cell concentration of 500 million /100 μl. Cell viability, determined by the method of staining with Trifanova blue (lang E.G. Stimulation of lymphocytes. // M: Medicine, 1976. - 288 S.)amounted to 96%. The method of determination of progesterone receptors in mononuclear blood cells based on the known method of determining these receptors in the cytosol fraction of bioerosion [Bassaleg. "Steroid hormone receptors in human tumors". M.: Medicine, 1987, str-202]. Time and concentration parameters of the saturation binding labels are chosen experimentally. For the quantitative determination of progesterone receptors in mononuclear cells prepared sterile plastic tablet (96 round-bottom wells): on all working wells contribute 10 ál alcohol solution3H-progesterone or3N-estradiol (Amersham GB) of receipt in the incubation medium final concentration of 20 and 5 nm, respectively. In order to inhibit specific binding of steroid use two hundredfold excess of unlabeled progesterone (Merck, Germany), which in an alcohol solution contribute to the wells according to the standard scheme. 200-fold excess of dexamethasone (Merck, Germany) is added to the working wells to inhibit cross-linking of glucocorticoids with the progesterone receptor. the donkey making holes alcohol solutions of these steroids alcohol is evaporated in a stream of nitrogen. 30 minutes before incubation in the wells contribute 50 ál environment Hanks. All the prepared wells contribute 50 μl of the suspension of mononuclear cells (500 million cells/100 µl) and incubated for 60 minutes for the progesterone receptor at room temperature. To separate the unbound label resuspendable cells filtered through glass filters (Whatman Glass Micro Fiber Filter GF/B - 25 mm dia.), who washed 100-fold excess of ice-cold Hanks and placed in scintillation vials. After evaporation filters pour 5 ml of scintillation fluid and after 60 minutes radiometric. All studies conducted in triplets. Counting specific binding produced by the formula: [(T-NSB)*k/n]*N, where T is the total binding, NSB - non-specific binding, n is the number of cells in a hole, k is the conversion factor cpm in femtomole3H-steroid, N is the number of Avogadro. Determination of progesterone receptor is carried out in the mid-luteal phase of the menstrual cycle of the patient, immediately before the appointment of an agonist of gonadotropin-releasing hormone prescribed when treatment is given for a standard long Protocol of stimulation of superovulation in IVF and embryo transfer. In accordance with the Protocol of stimulation of superovulation can be used, for example, the following agonists gonadotropin-releasing hormone: the trip is Orlin, goserelin, buserelin. When the content of the progesterone receptor by more than 700 of the progesterone receptor on the cell predict the onset of pregnancy as a result of the IVF program and PE in standard long Protocol of stimulation of superovulation. We present data supporting the ability of its stated purpose and achievement of the technical result. Presented concrete examples of the implementation of the claimed method. Example No. 1. Patient Century. 27 years repeatedly asked the Department of in vitro fertilization Center for family planning and reproduction No. 1 in October 2006 with a diagnosis of secondary infertility the duration of 5 years, male factor, tubal-peritoneal factor for the second attempt in vitro fertilization and embryo transfer. History 1 unsuccessful attempt in vitro fertilization and embryo transfer at the short Protocol with antagonists of gonadotropin releasing hormone in August 2006, an ultrasound of the pelvic organs found normal follicular reserve, in the future when the puncture was obtained 14 oocytes. On the 21st day of the menstrual cycle (mid-luteal phase), before assigning the patient an agonist of gonadotropin-releasing hormone - daily subcutaneous injection Diveroli is as 0,1, was extracted peripheral blood from the cubital vein to determine the level of progesterone receptors in mononuclear fraction of peripheral blood cells, which amounted to 20545 receptors on the cell. Thus, the results of the receptor profile of cells of the mononuclear fraction of peripheral blood, let us assume favorable for this patient, the prognosis for pregnancy in the standard long Protocol of stimulation of superovulation in IVF and embryo transfer, as indicated in the following: after transfer into the uterus of two embryos of good quality came singleton pregnancy ended urgent delivery and birth of live full-term girls. Similar positive results were obtained in 18 (out of 24 pregnant) patients. Example No. 2. Patient K., 31 years primarily addressed to the Department of in vitro fertilization Center for family planning and reproduction No. 1 in October 2007 with a diagnosis of secondary infertility duration 7 years, tuboperitoneal tubal factor for the first attempts of in vitro fertilization and embryo transfer. An ultrasound of the pelvic organs found normal follicular reserve, in the future when the puncture was obtained on 16 oocytes. On 20 de is ü menstrual cycle (mid-luteal phase) before assigning the patient an agonist of gonadotropin-releasing hormone: a daily subcutaneous injection of Diphereline 0,1, was extracted peripheral blood from the cubital vein to determine the level of progesterone receptors in mononuclear fraction of peripheral blood cells, which amounted to 574 receptor on the cell. Thus, the results of the receptor profile in the mononuclear fraction of peripheral blood cells has allowed us to assume unfavorable for this patient, the prognosis for pregnancy in standard long Protocol of stimulation of superovulation in IVF and embryo transfer. This was confirmed in the following: pregnancy has not occurred, despite the selective transfer on the 5th day 2 embryos of good quality in the uterus. Similar negative results were obtained in 13 (out of 16 are not pregnant) patients. Thus, when using the proposed method in 40 patients younger than 39 years was confirmed by its high accuracy and the ability to use for the purposes of forecasting the onset of pregnancy. The prediction was confirmed in 31 of patience, i.e. in 77.5% of cases. A method for predicting pregnancy in in vitro fertilization and embryo transfer in the standard long Protocol of stimulation super is valatie, characterized in that examine the hormonal status of the patient, which in the mid-luteal phase of the menstrual cycle of the patient, before the appointment of an agonist of gonadotropin-releasing hormone during the treatment according to the standard long Protocol of stimulation of superovulation, determine the content of progesterone receptors in mononuclear fraction of peripheral blood cells and in the amount of more than 700 of the progesterone receptor on the cell predict the onset of pregnancy as a result of programs of in vitro fertilization and embryo transfer in the standard long Protocol of stimulation of superovulation.
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