Method of predicting development of cerebral metastases in case of lung cancer in women |
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IPC classes for russian patent Method of predicting development of cerebral metastases in case of lung cancer in women (RU 2439579):
Method of diagnosing androgenic deficiency / 2439578
In blood serum bioavailable testosterone fraction bound to albumin is determined. If its value is lower than 185.7 pg/ml, disease is diagnosed.
 Method of predicting survival potential of patients with malignant gliomas / 2439577
In patient's urine content of 6-sulfatoximelatonin is determined. If determined content of 6-sulfatoximelatonin after operation before complex treatment equals 292.7 nmol/ml survival to 2.3±0.5 months is predicted, if 22.4 nmol/ml is determined, survival for more than 12 months is predicted. If after chemical therapy determined content of 6-sulfatoximelatonin equals 268.2 nmll/ml, predicted survival time equals to 2.1±0.6 months, if content is 32.8 nmol/ml - more than 12 months. If after a month after treatment determined content of 6-sulfatoximelatonin equals 479.3 nmol/ml predicted is survival for 1.2±0.5 months, in case of 37.4 nmol/ml - survival for more than 12 months.
Method for prediction of cervical cancer metastases / 2436102
In 10-14 days following a surgical removal of a tumour in a patient, daily urine is analysed for sex hormones: oestradiol and pregnandiol. Then, they are related to each other. If the value exceeds 0.8, the onset of metastases or a recurrent disease for the following one and a half or two years is predicted. If the relation is less than 0.8, a metastases-free and recurrence-free period for more than 8-10 years is predicted.
Method of predicting state of fertility in women of reproductive age with uterus myoma / 2433411
At lutein phase of menstrual cycle concentrations of 2-hydroxyestrone 16α - hydroxyestrone in urine, concentration of progesterone in blood serum are determined. Then, canonical value is calculated by formula: K=4.23-0.04×A+0.0016×B-0.18×C, where A is concentration of progesterone in blood serum on 20-21 days of menstrual cycle (lutein phase) (nmol/l), B - concentration of 2-hydroxyestrone in urine (nmol/l), C - concentration of 16α - hydroxyestrone in urine (nmol/l). If K is higher than -0.175, infertility is predicted, if K is lower than -0.175 - safe fertility.
Method of predicting pregnancy in programme of extracorporal fertilisation and transfer of embryos in standard long protocol of superovulation stimulation / 2430379
Before programme of ECF and TE in standard long protocol of superovulation stimulation in patient analysis of hormonal status is performed - content of progesterone receptors in mononuclear fraction of peripheral blood cells is determined. Content of progesterone receptors in mononuclear fraction of peripheral blood cells is determined in the middle of luteal phase of patient's menstrual cycle, before administration of gonadotropin-releasing hormone agonist when carrying out treatment in accordance with standard long protocol of superovulation stimulation. If value is more than 700 progesterone receptors per cell, pregnancy as a result of programme of ECF and TE in standard long protocol of superovulation stimulation is predicted.
Method for prediction of clinical effectiveness of metformin and/or weight-loss therapy in patients with polycystic ovarian syndrome / 2427842
Patient is examined for an anti-Mueller hormone level. If its value is increased by 1/3 and less from an upper limit of normal, a positive menstrual response to treatment is predicted.
Method of predicting risk of thyroid gland diseases in women of perimenopausal age / 2421127
Invention relates to field of medicine, namely to endocrinology. The following data are determined: cause of menopause, application of estrogen preparations, value of index of body weight and thyroid gland volume, concentration of antibodies to thyroid peroxidase and thyrotropic hormone. On the basis of obtained data prognostic coefficient which makes it possible to predict presence or absence of risk of developing thyroid gland diseases in the nearest five years is calculated.
 Method for prediction of developing metastases in patietns suffering rectal cancer / 2420742
Tissue samples of an tumour and perifocal regions are recovered from the preparation removed intraoperatively. They are analysed for the contents of prolactin and testosterone by radioimmunoassay. If the prolactin concentration is 692.0 ng/g of tissue, and the testosterone concentration is 48.0 ng/g of tissue in the tumour, while the testosterone concentration is 42.0 ng/g of tissue in the perifocal region, developing hepatic metastases are expected in 3 months. If the prolactin concentration is 160-390 ng/g of tissue, and the testosterone concentration is 20-40 ng/g of tissue in the tumour, while the testosterone concentration is 24-26 ng/g of tissue in the perifocal region, developing metastases are expected in 29-43 months.
 Diagnostic technique for function-type oddi's sphincter dyssynergia following cholecystectomy / 2416802
Cholecystokinin level is counted additionally in the patients with post-cholecystectomy syndrome. In case the concentration is below 0.5 ng/ml, and while observing no organic pathology of hepatopancreatoduodenal organs as demonstrated by instrument methods, function-type Oddi's sphincter dyssynergia is diagnosed.
Method of predicting duration of recurrence-free period in radically treated patients with mammary gland cancer / 2413231
Content of sex hormones -estradiol and progesterone - in blood is determined. After that coefficient of ratio of estradiol concentration to progesterone concentration is calculated. If values of coefficient are within the range from 10 to 221, duration of recurrence-free period from 5 to 13 years is predicted, and if values of coefficient are within the range 367-1295 recurrence-free period for 28-30 months is predicted.
Method of predicting efficiency of treating miscarriages / 2439571
On 6-12 week of gestation in peripheral venous blood of pregnant women with threat of miscarriage before treatment relative content of CD4+CD25+ lymphocytes is determined and if its value is less than 4.0%, short-term effect of standard preserving therapy is predicted, and if value equals 4.0% and higher, prolonged effect of preserving therapy and course of pregnancy without threat of abortion in 2 and 3 trimesters is predicted.
Method of estimating antiasthmatic therapy efficiency / 2439570
In patient obtaining basic anti-inflammatory therapy with combination of inhalation glucocorticosteroid and β2-aginist of long action, level of obtaining control of bronchial asthma is determined by criteria GINA 2006. After that, additionally determined is expression of CD20 marker. If CD20 expresses 16% and more, carried out anti-asthmatic therapy is considered ineffective, requiring correction, if less than 16% of cells - effective.
Method of predicting bronchopulmonary displasia in children with inherent pneumonia / 2439569
In newborn babies with intrauterine pneumonia, who are on artificial lung ventilation, content of protein of cells Clara (CC 16) is analyses on the 3-5-th day of life in single portion of bronchoalveolar lavage and if its value is less than 53 ng/ml, development of bronchopulmonary dysplasia is predicted, when there are no clinical manifestations. Claimed method possesses high accuracy, sensitivity, specificity.
 Strain "stavropol 01/08" of african swine plague virus for virological, molecular-genetic and monitoring research / 2439152
Strain was isolated from the pig which died in LLP "Gorkaya Balka" in Soviet district of Stavropol region during disease epizootic in 2008. Strain is new, earlier unknown, isolated on the territory of RF and is designated as strain "Stavropol 01/08". It possesses high infectious activity, accumulates in cultures of bone marrow cells of pigs and pigs' leukocytes in titre 6.0-7.0 lg HAU50 cm3. Virus titre on pigs constitutes 7.0-7.5 lg LD50 cm3. Death of infected pigs occurs with symptoms characteristic of acute form of ASP 3-6 days after manifestation of clinical symptoms of disease. Invention can be used in research institutions and diagnostic centres as reference-strain in carrying out virological, molecular-genetic and monitoring research.
 Immunocytochemical estimation method for proliferative lymphocyte state by expression behaviour of c-terminal b23/nucleophosmin protein fragment in indirect immunofluorescence test / 2438135
Immunocytochemical estimation method for proliferative lymphocyte state by an expression behaviour of an C-terminal B23/nucleophosmin protein fragment in indirect immunofluorescence test provides a visual cell estimation for count and size of luminous nucleoli marked by a specific anti-peptide antibody to the C-terminal B23/nucleophosmin protein fragment detecting a B23.1 protein oligomer form only of molecular weight 210-230 kDa. The intact lymphocyte value found in a cycling state G0 is the presence in a cell nucleus of no more than one luminous nucleoli detected by the specified C-fragment B23 antibody, while 2-3 luminous nucleoli are detected in lymphocytes in the process of proliferative lymphocyte activity caused by PHA stimulation in 24 hours from the initiation of the stimulation procedure, and 4-5 luminous nucleoli of greater size and more intense luminescence between + to +++ as compared with the reference are detected in the cells in 48-72 hours from the initiation of the stimulation procedure when most lymphocytes are found at the peak of proliferative activity in the cycling state S.
Method for prediction of clinical effectiveness in acute rhinosinusitis / 2438134
Chemoluminescence is used to analyse functional activity of neutrophilic granulocytes in patient's peripheral blood, to evaluate an activation index representing the relation of the area under a curve of lucigenin-dependent zymosan-induced chemoluminescence of neutrophilic granulocytes to the area under a curve of spontaneous lucigenin-dependent chemoluminescence of neutrophilic granulocytes. If the activation index exceeds 3.6, high clinical effectiveness of a standard therapy is predicted, while the activation index being 3.6 and less enables to predict low clinical effectiveness or the absence of clinical effectiveness of the standard therapy.
Method for prediction of clinical effectiveness in glucocorticoids treated multiple sclerosis / 2438133
Invention describes a method for prediction of clinical effectiveness in glucocorticoids-treated multiple sclerosis by blood examination wherein blood is analysed for absolute apoptotic lymphocyte count; a programmed cell death realisation index (%) is calculated prior to, during and after the treatment by formula, and if observing the increase of the apoptosis realisation index (ARI) after glucocorticoid treatment in relation to its initial values, the treatment is considered to be effective, while the absence of dynamics enabled to predict a negative efficacy of the hormonal therapy.
 Method of imaging of astroglial bank in diagnosing of high-grade gliomas / 2437159
Delimitation of a high-grade glioma invasion is ensured by imaging of an astroglial bank surrounding the high-grade glioma. An immunogenic recombinant human GFAP is prepared and used to immunise a Balb/C mouse; spleen B-lymphocyte of this mouse are recovered and fused with myeloma cells of Sp 2/0-Ag14 mice; hybridomas are produced. Supernatants of the prepared hybridomas are tested by immunochemical techniques for the presence of anti-GFAP antibodies used to select a hybrid cell clone producing the anti-GFAP antibodies able to distinguish GFAP in vivo. The anti-GFAP antibodies are cleaned from the supernatant of the selected clone and covalently bound with liposomal nanocontainers containing a diagnostic mark. The antibodies of the selected hybrid cell clone is modified by g-amino groups of lysine residues and incubated with the stelths-liposome solution. The prepared nanosystem is introduced in a patient's vascular bed, and the astroglial bank is imaged by the arrangement of the diagnostic mark in cerebral tissues.
Method for instant determination of blood atherogenicity (versions) / 2437098
Blood plasma is process in a medium containing 10% polyvinylpyrrolidone. Blood atherogenicity is stated visually if observing opacity of an examined sample. The use of the method allows carrying out screening in a relatively short time and detecting people with propensity for atherosclerosis. In-depth study of such patients shall allow detecting an individual reason of blood atherogenicity and performing etiotropic therapy at the pre-clinical stages of atherosclerosis.
 Method of detection of specific staphylococcus ige-antibodies in blood serum of patient with allergic diseases, hypersensitive to staphylococcus allergens, with using test system for detection of specific staphylococcus ige-antibodies, active allergen substance (aas) recovered from staphylococcus and diagnostic technique for staphylococcus allergy / 2436100
Invention provides an IFA test system for detection of specific staphylococcus IgE-antibodies in blood serum of a patient with allergic diseases, hypersensitive to staphylococcus allergens; a method for detection of specific staphylococcus IgE-antibodies in blood serum of the patient with allergic diseases, hypersensitive to staphylococcus allergens, with using said test system; and a diagnostic technique for staphylococcus allergy.
 Method for predicting the character of bacterial keratitis flow / 2245553
In lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula:
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FIELD: medicine. SUBSTANCE: in women in tissue of lung malignant tumour after radical surgery in pulmonectomy volume and in intact lung tissue, obtained from ablated from lung section of the same lung, level of progesterone is determined. If it reduces in tumour in 5 times and more relative the level of progesterone in intact lung tissue, development of cerebral lung cancer metastases in women within the term from 3 to 8 months is predicted. EFFECT: possibility of early prediction of development of cerebral metastases in women with lung cancer, which gives possibility of take adequate preventive therapeutic measures. 2 ex
The invention relates to medicine, namely to Oncology, and can be used to detect patients with likelihood of metastasis of lung cancer to the brain and the appointment of adequate treatment. There is a method of determining the criteria for identifying the likelihood of metastatic brain cancer at various sites (sacon p. g Development of organizational forms of diagnosis of metastatic lesions of the Central nervous system. Abstract of Diss. ... candles. the honey. of Sciences, Rostov-on-don, 2006). The author on the basis of the analysis of clinically established cases of detection of metastases of various cancers in the brain, as well as metastases detected on autopsy, defined General criteria of probability of detection of metastatic lesions of the brain. Such criteria for lung cancer patients had a long history of less than 1 year, the initial stage of the process, namely T2-3N1-2Mxage 50-70 years, the peripheral shape of the tumor growth, histological type adenocarcinoma, the presence of a paraneoplastic syndrome. However it is noted that the frequency of metastatic CNS lesions in women was 4 times lower than those of men. However, the present study was conducted with the analysis of the clinical material of patients with already having what I metastases to the brain or autopsy material. The author is not the results of the use of the identified criteria for prediction of the development of metastases in particular women, lung cancer patients. Not included in the risk group and patients with small tumors in the lung, without associated lymph nodes (T2N0). The method for determination of biochemical markers in serum and cerebrospinal fluid in patients with intracranial tumors, including tumors of glial and metastatic nature (Boyardi A., Munari L. Silvani A., Solero C.L., bombardieri E. Neuron specific enolase (NSE) and Thymidine kinase (TK) as markers in diological fluids of brain tumor patients. // Ital. J. Neurol. Sci - 1990. - 11, N.4. - C.359-366). This method was chosen as a prototype. The authors were examined in 104 patients with diseases of the nervous system, including glial tumors and metastases of various cancers. Detected a significant increase in the activity timedancing in the serum of most patients with tumors of primary and secondary nature. Increased levels timedancing in serum and cerebrospinal fluid indicated the presence of metastatic lesions of the brain. However, as noted by the authors of the study, the sensitivity of the method was 63%, a specificity of 36%, and it is not possible to consider the analyzed indicator pathogenetically determined and used to forecast the development of metastases in the brain malignant process easy. Researchers, mainly recommend using activity indicator timedancing in serum as a marker of disease recurrence after surgical treatment of patients with intracranial tumors. The aim of the invention is the possibility of early detection in women, lung cancer patients, metastatic lesions of the brain. This objective is achieved in that in the tissue of malignant lung tumor after radical surgery in volume pulmonectomy in tissue taken from a remote from the tumor site in the same light, determine the level of progesterone and by reduction in tumor tissue in 5 times or more relative to the intact lung tissue predict the occurrence of cerebral metastases of lung cancer in the period from 3 to 8 months. The invention of "a method for predicting the development of cerebral metastases in lung cancer in women is new, because it is unknown the level of medicine in the field of forecasting the possibility of developing distant intracranial metastases of malignant process easy. The novelty of the invention lies in the fact that in tumor tissue to determine the level of progesterone and its reduced more than 5 times predict the likelihood of metastasis of lung cancer to the brain. This method for predicting the Oia is new and gives the opportunity to take timely measures to prevent the development of distant metastasis of a tumor. In open sources information of Russia, CIS and abroad indications for a similar method for predicting the development of cerebral metastases of lung cancer in women, we have not identified. The invention is industrially applicable as it can be reproduced and repeated many times in the medical health care institutions. The method is as follows. A portion of the tumor tissue of the lung in the amount of 100 mg was ground in a homogenizer with 1 ml of cold saline. The obtained homogenate was centrifuged for 30 min at 18000 rpm Precipitate was discarded, and the supernatant was determined by the level of progesterone radioisotope method using sets "Immunotech a Beckman coulter company. As a control sample (intact lung tissue) used a sample of tissue taken from the distant from the tumor site to the same remote lung. Immunoradiometric determination of progesterone refers to the competitive analysis. The analyzed samples and calibration samples incubated with progesterone labelled125I in test tubes coated with antibodies. After incubation, remove the liquid contents of the test tubes and measure related activity125I. progesterone Concentration in the samples is determined by interpolation on the calibration curve. Were IP is ledovyy 20 female patients, were treated in the thoraco-plastic branch of FGU "RNII of gynecology and subjected to surgical treatment regarding Central or peripheral lung cancer T2N0-1M0. Found that 5 patients from 20 value content of progesterone in the tumor tissue was varied in the range of 35.2±4.1 ng/g of tissue against the metric defined in the intact lung tissue - 258,2±21.5 ng/g tissue, which was accompanied by the development of these patients have metastases to the brain within the next 8 months (from 3 to 8 months). An example of the application method can serve as a statement of case histories. Patient B., born in 1942, history No. 15821/H, has been with the Department of thoraco-plastic surgery, FSU RNII and gynecology" 29.07.2008 diagnosed with a tumor in the left lung. When contacting the Institute complained of cough without blood, shortness of breath, weakness. On the radiograph 29.07.08 detected Central tumor of the upper lobe of the left lung with the transition to the distal section of the main bronchus. Bronchoscopically 30.07.08: main bronchus in the bottom third on 4/5 blocked exophytic, hilly, dense formation, which comes from the upper lobe bronchus. Cytologically (No. 16865) and histologically (345702-03/08) - squamous cell carcinoma. 07.08.08. the patient underwent an operation in the amount of: left pulmonectomy histological analysis No. 47724-29/08 - moderately-differentiated squamous non-squamous cancer metastases in lymph nodes - no). Intraoperatively, the patient was taken to study samples of lung tissue: from the tumor and from the zone that is located remotely from the tumor (intact tissue). From the obtained samples were prepared 10% homogenates, which determined the level of progesterone radioisotope method using sets "Immunotech a Beckman coulter company. In tumor tissue levels of progesterone was 100 ng/g tissue in the intact tissue - 220 ng/g tissue, i.e. in the tumor rate was only 2.2 times lower than in the unaffected lung tissue. After 1 year and 2 months the patient alive during the next inspection data for recurrence and metastatic process no. Obtained in the study of progesterone levels in tumor tissue compared with the values in the intact tissue corresponds to the predicted favorable prognosis of the disease. Patient P., born in 1956, case history No. 25043/H, was admitted to the thoracic Department, FSU RNII and gynecology" 02.12.2008 diagnosed with a tumor in the left lung. Sick since September 2008, was treated at the place of residence for chronic bronchitis with cough without blood, shortness of breath, fever up to 37,2-to 37.9°C. When bronchoscopic study 04.12.2008 found peribronchial-nodular tumor of the upper lobe of the left lung (resultative No. 73958 - adenocarcinoma). Rentgenograficheski: 28.11.2008 - Central tumor of the upper lobe of the left lung 3.0 cm in diameter and the right upper lobe dense tbc foci (since 2004 without speakers). 09.12.08 produced pulmonectomy left. Assigned to symptomatic therapy. During surgery, the patient was extracted tissue samples from the tumor and contralateral (intact) zone is easy, located away from the tumor tissue. From the obtained samples were prepared 10% homogenates, which determined the level of progesterone radioisotope method using sets "Immunotech a Beckman coulter company. In the study produced the following results: progesterone levels in tumor tissue - 28 ng/g tissue in the intact tissue - 261 ng/g tissue, i.e. in the tumor rate was 9.3 times lower than in the unaffected lung tissue. After 3 months (11.03.09) the patient had complained of pain in the lumbar spine with paresthesia lower extremities. When radiographic examination revealed metastatic lesions of the spine, recommended an MRI of the brain and of the thoracolumbar spine in which 24.03.09 detected metastatic brain lesion (right temporal lobe) and the thoracic spine. In this study, the decrease in the value of progesterone in tumor tissue compared to intact the cloth 9.3 times pointed to a possible metastasis of lung cancer to the brain, and after 3 months, the patient is established metastatic brain damage. Technical and economic efficiency of the method is the ability to timely forecasting of the development of cerebral metastases in women, lung cancer patients, and taking appropriate preventive therapeutic measures. A method for predicting the development of cerebral metastases in lung cancer in women, including Immunoradiometric research in tissue malignant tumors of the lung, characterized in that tissue malignant lung tumor after radical surgery in volume pulmonectomy in tissue taken from a far distant from the tumor site in the same light, determine the level of progesterone and by reduction in tumor 5 times or more relative to the intact lung tissue, predict the development of metastasis of lung cancer to the brain in the period from 3 to 8 months.
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