Method for prediction of cervical cancer metastases |
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IPC classes for russian patent Method for prediction of cervical cancer metastases (RU 2436102):
Method of predicting state of fertility in women of reproductive age with uterus myoma / 2433411
At lutein phase of menstrual cycle concentrations of 2-hydroxyestrone 16α - hydroxyestrone in urine, concentration of progesterone in blood serum are determined. Then, canonical value is calculated by formula: K=4.23-0.04×A+0.0016×B-0.18×C, where A is concentration of progesterone in blood serum on 20-21 days of menstrual cycle (lutein phase) (nmol/l), B - concentration of 2-hydroxyestrone in urine (nmol/l), C - concentration of 16α - hydroxyestrone in urine (nmol/l). If K is higher than -0.175, infertility is predicted, if K is lower than -0.175 - safe fertility.
Method of predicting pregnancy in programme of extracorporal fertilisation and transfer of embryos in standard long protocol of superovulation stimulation / 2430379
Before programme of ECF and TE in standard long protocol of superovulation stimulation in patient analysis of hormonal status is performed - content of progesterone receptors in mononuclear fraction of peripheral blood cells is determined. Content of progesterone receptors in mononuclear fraction of peripheral blood cells is determined in the middle of luteal phase of patient's menstrual cycle, before administration of gonadotropin-releasing hormone agonist when carrying out treatment in accordance with standard long protocol of superovulation stimulation. If value is more than 700 progesterone receptors per cell, pregnancy as a result of programme of ECF and TE in standard long protocol of superovulation stimulation is predicted.
Method for prediction of clinical effectiveness of metformin and/or weight-loss therapy in patients with polycystic ovarian syndrome / 2427842
Patient is examined for an anti-Mueller hormone level. If its value is increased by 1/3 and less from an upper limit of normal, a positive menstrual response to treatment is predicted.
Method of predicting risk of thyroid gland diseases in women of perimenopausal age / 2421127
Invention relates to field of medicine, namely to endocrinology. The following data are determined: cause of menopause, application of estrogen preparations, value of index of body weight and thyroid gland volume, concentration of antibodies to thyroid peroxidase and thyrotropic hormone. On the basis of obtained data prognostic coefficient which makes it possible to predict presence or absence of risk of developing thyroid gland diseases in the nearest five years is calculated.
 Method for prediction of developing metastases in patietns suffering rectal cancer / 2420742
Tissue samples of an tumour and perifocal regions are recovered from the preparation removed intraoperatively. They are analysed for the contents of prolactin and testosterone by radioimmunoassay. If the prolactin concentration is 692.0 ng/g of tissue, and the testosterone concentration is 48.0 ng/g of tissue in the tumour, while the testosterone concentration is 42.0 ng/g of tissue in the perifocal region, developing hepatic metastases are expected in 3 months. If the prolactin concentration is 160-390 ng/g of tissue, and the testosterone concentration is 20-40 ng/g of tissue in the tumour, while the testosterone concentration is 24-26 ng/g of tissue in the perifocal region, developing metastases are expected in 29-43 months.
 Diagnostic technique for function-type oddi's sphincter dyssynergia following cholecystectomy / 2416802
Cholecystokinin level is counted additionally in the patients with post-cholecystectomy syndrome. In case the concentration is below 0.5 ng/ml, and while observing no organic pathology of hepatopancreatoduodenal organs as demonstrated by instrument methods, function-type Oddi's sphincter dyssynergia is diagnosed.
Method of predicting duration of recurrence-free period in radically treated patients with mammary gland cancer / 2413231
Content of sex hormones -estradiol and progesterone - in blood is determined. After that coefficient of ratio of estradiol concentration to progesterone concentration is calculated. If values of coefficient are within the range from 10 to 221, duration of recurrence-free period from 5 to 13 years is predicted, and if values of coefficient are within the range 367-1295 recurrence-free period for 28-30 months is predicted.
Method of predicting of process activation in patients with hodgkin's lymphoma / 2405454
Invention relates to medicine, namely to biochemical investigations in oncology, and can be used in determination of activation or stabilisation of pathologic process in patients with primary-resistant Hodgkin's lymphoma. In patients with primary-resistant Hodgkin's lymphoma at stages of treatment, by radioimmune method determined is content in blood of thyroid gland hormones - triiodothyronine and thyroxine, and adrenocortical hormone - cortisol, their concentration is summed up and in case of total level of hormones is within 278.2 nmole/l - 0 333.0 nmole/l conclusion about activation of malignant process is made, if total level of said hormones is 432.4 nmole/l - 833.2 nmole/l conclusion about absence of process activation is made.
Diagnostic technique for severity level of varicocele and its recuring / 2403871
Invention refers to medicine, namely to urology and vascular surgery. The sex hormones level in an involved vein is considered to be a criterion of the severity level of varicocele. There are evaluated the following sex hormones: testosterone, estradiol, progesterone simultaneously in the pampiniform plexus veins of a left ovary and in the peripheral vein of a bend of elbow, with determining the ratio of the sex hormones level in a healthy vein of the bend of elbow to the varicocele-involved pampiniform plexus vein. If said ratio of the sex hormones level is 1:10 to 1:50, the first degree of varicocele is diagnosed. Recurrent varicocele and the second degree of varicocele is diagnosed by the ratio of the sex hormones level in the healthy and involved veins 1:50 to 1:100 times (i.e. hormones concentration in a varicose vein is increased in tens times). If observing the ratio of the sex hormones level in the healthy vein and the involved vein of the pampiniform plexus veins of the left ovary increasing more than in 1:100, the third degree of varicocele is diagnosed. Surgical intervention is indicated if the ratio of the sex hormones level is diagnosed more than 1:50.
 Method of bone resorption to remodelling analysis / 2403870
Invention refers to medicine, namely to biochemistry, surgery and dentistry. The bone resorption to remodeling analysis is based on the biochemical blood examination. An the blood coefficient K1 is calculated by formula: K1=[(PTH:A)+(TNF-α:B)+(IL-1β:C)]:3, where PTH is the parathyroid hormone concentration (pg/ml) in the patients, A is the same value in healthy individuals, TNF-α is the level of tumor necrosis factor -α (pg/ml) in the patients, B is the same value in healthy individuals, IL-1β is the interleukin-1β concentration (pg/ml) in the patients, C is the same value in healthy individuals to derive the blood coefficient K2 by formula: K2=[(calcitonin: D) + (osteocalcin: E)]:2, where the calcitonin concentration (ng/ml) in the patients, D is the same value in healthy individuals, the osteocalcin level (ng/ml) in the patients, E is the same value in healthy individuals. Then the bone metabolism control coefficient (BMCC) is calculated by formula BMCC=K1:K2, and the higher BMCC than 1.17, the more intensive resorption prevails over remodelling; and the lower BMCC than 0.83, the more intensive remodelling prevails over resorption.
 Method for prediction of osteogenesis disturbance in long bone fractures / 2436097
Growth factor (TGFβ1) level, a lymphocyte-thrombocyte adhesion (LTA) value are determined; microcirculation parameters (maximum vibration amplitude of myogenic, neurogenic and respiratory blood flow components) of an injured extremity are recorded on the 10th postoperative day. Further, their relative values related to average values in health are determined, and coefficient K is calculated by formula K=P1;P2;P3, where P1 is a relative TGFβ1 content; P2 is a relative LTA content; P3 is relative values of a product of the maximum amplitudes of neurogenic, myogenic and respiratory microcirculation components. If the coefficient is less than 0.9, osteogenesis disturbance is predicted.
Method for evaluating effective iron transport in patient with renal insufficiency / 2436096
Patient's blood serum is analysed for the concentration of transferrin and iron metabolism values. The effective transport iron concentration (ETC) is calculated by formula: ETC = (CTr·CI)/( CI+UIBC), g/l, where CTr is the transferrin concentration, g/l; CI is the serum iron concentration, mcmol/l; UIBC is the unsaturated iron-binding capacity, mcmol/l. If the ETC value is within 0.7 g/l and higher, it is considered as adequate, while the value less than 0.70 shows the deficient iron transport.
Method for prediction of uterine leiomyoma necrosis in pregnant women in their second trimester / 2436092
Blood leukocyte level, erythrocyte sedimentation rate, blood fibrinogen level are measured. A prognostic index is calculated by formula Z=0.217×X1+0.083×X2+0.458×X3, where X1 is leukocytes - L (109/l), X2 is erythrocyte sedimentation rate - ESR (mm/h), X3 - venous blood fibrinogen - FG (g/l). If the Z value is less than 6.96, non-necrosis uterine myoma is predicted; if the Z value exceeds 7.80, uterine myoma necrosis is predicted, and the Z value within the range 6.96 to 7.80 provides uncertainty and requires progression follow-up of a patient.
 Polypeptide, possessing inhibiting activity with respect to platelet aggregation and/or inhibiting activity with respect to platelet adhesion, encoding it polynucleotide, composition and set, containing said polypeptide / 2434941
Polypeptide, which is used in composition of pharmaceutical composition and in sets for screening of adhesion inhibitors of platelet adhesion or aggregation, is obtained in recombinant way applying matrix of cDNA of Anopheles stephensi salivary gland.
 Method of hystochemical detection of methacide in liver in case of poisoning with polymer-based surrogate alcohol / 2433409
Method includes preparation of paraffin cut, deparafination, staining with 2% solution of congo red in 10% alcohol, washing in water, analysis of micropreparations of liver by light microscope. Preliminarily review microscopy is performed, micropreparations with suspicion on toxic hepatitis with intralobular cholestasis are sorted out, staining with congo red is carried out during 10-15 minutes, after washing in water micropreparation of liver is placed into glycerol, after that it is analysed under microscope and in case if orange lining is visualised around bile inclusions and cylinders and irregular orange staining of cytoplasm of hepatocytes, presence in liver tissue of methacide (polyhexamethylene guanidine hydrochloride) is determined.
Method of predicting development of pollen bronchial asthma / 2433408
In patient's venous blood antigens of locus A and B of HLA system are defined. In case if double-locus haplotypes HLA-A2/B35 and/or A10/B35, and/or A19/B16 and/or A19/B35 are detected, high risk of development of pollen bronchial asthma is predicted. If double-locus haplotypes HLA-A2/B7 and/or A3/B7 and/or A11/B7 are present, resistance to development of pollen bronchial asthma is predicted.
 Methods for modulating cell-surface receptors for prevention or reduction of inflammation / 2431845
At least one bacterium or a portion of a bacterium of oral bacteria species contacts with a gingival cell, and the presence of an indicator material is detected. The indicator material may be selected from a group consisting of molecules generated by modulation of a toll-like receptor or a NFk-B path. The absence of the indicator material shall mean that the given bacteria species is not malignant.
Method for prediction of development of hepatohephric syndrome in acute abdominal surgical pathology / 2431840
Patient is analysed for a leukocytic intoxication index, a neutrophilic index, an average-weight molecule, creatinine, urea, cytolysis enzymes, coagulogram values, a thrombocyte level, the presence of intestinal insufficiency syndrome, urine passage. Each value is appraised by 1 point; the points are summed up. If detecting 3-5 values (point total 3-5), a risk of hepatic and renal complications is predicted, 6-9 markers (6-9 points) enable predicting development of a latent phase of hepatorenal syndrome; detecting all 10 markers (10 points) provides to predict transformation of a latent phase of hepatorenal dysfunction in clinically manifested one.
Method to detect bacteria pasteurella trehalosi and/or mannheimia haemolytica in poultry (versions) / 2430967
Method includes the following stages: getting a sample from a poultry's organism, where the sample is selected from the group, including blood, serum, plasma, tissue scrapes, swabs, smears, tissue or separation of bacteria proteins selected from the group Pasteurella trehalosi and/or Mannheimia haemolytica, which are deposited under the registration numbers ATSS No. RTA-3667, ATSS No. RTA-3668, ATSS No. RTA-3669, with the help of gel electrophoresis on polyacrylamide gel and their transfer to a suitable carrier; incubation of the specified sample or the carrier with antibodies specific in respect of bacteria selected from the group Pasteurella trehalosi and/or Mannheimia haemolytica, which are deposited under the registration numbers ATSS No. RTA-3667, ATSS No. RTA-3668, ATSS No. RTA-3669, and detection of availability of antigen-antibody complex.
Method of antibiotic therapy correction in patient of cardiosurgical profile / 2430373
In order to correct antibiotic therapy in patients of cardiosurgical profile in early post-operation period, starting from 2-14 day after operation on heart, performed in conditions of artificial blood circulation, samples for microbial flora analysis are taken from not less than five different area from the following group: skin of palm surface of hand, skin in area of elbow bend, skin in zone of post-operation suture, skin in zone of contact with medicinal and/or diagnostic devices, surfaces of said devices in zone of contact with upper half of body. Composition of microbial flora is samples is analysed. If in at least one probe Gram-negative microorganisms, related to at least one of the following genera Pseudomonas spp, Acinetobacter spp, Klebsiella spp, Enterobacter spp, Serratia spp, Citrobacter spp, Escherichia spp and/or S.Aureus, are isolated, estimation of their sensitivity to antibiotics, obtained by patient, and/or antibiotics-candidates for inclusion into antibiotic therapy, is carried out. For correction of antibiotic therapy selected is the antibiotic or antibiotics, to which isolated microorganisms are sensitive.
Method for evaluating the severity of pathological process flow at chronic diffuse hepatic lesions / 2244305
One should detect the level of hepato-specific enzymes (HSE) in blood plasma, such as: urokinase (UK), histidase (HIS), fructose-1-phosphataldolase (F-1-P), serine dehydratase (L-SD), threonine dehydratase (L-TD) and products of lipid peroxidation (LP), such as: dienic conjugates (DC), malonic dialdehyde (MDA). Moreover, one should detect the state of inspecific immunity parameters, such as: immunoregulatory index (IRI) as the ratio of T-helpers and T-suppressors, circulating immune complexes (CIC). Additionally, one should evaluate the state of regional circulation by applying rheohepatography (RHG), the system of microhemocirculation with the help of conjunctival biomicroscopy (CB) to detect intravascular index (II). In case of increased UK, HIS levels up to 0.5 mcM/ml/h, F-1-P, L-SD, L-Td, LP products, CIC by 1.5 times, higher IRI up to 2 at the norm being 1.0-1.5, altered values of regional circulation, increased II up to 2 points at the norm being 1 point, not more one should diagnose light degree of process flow. At increased level of UK, HIS up to 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 1.5-2 times, increased IRI up to 2.5, altered values of regional circulation, increased II up to 3-4 points one should diagnose average degree of process flow. At increased level of UK, HIS being above 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 2 and more times, increased IRI being above 2.5, altered values of regional circulation, increased II up to 5 points and more one should diagnose severe degree of process flow.
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FIELD: medicine. SUBSTANCE: in 10-14 days following a surgical removal of a tumour in a patient, daily urine is analysed for sex hormones: oestradiol and pregnandiol. Then, they are related to each other. If the value exceeds 0.8, the onset of metastases or a recurrent disease for the following one and a half or two years is predicted. If the relation is less than 0.8, a metastases-free and recurrence-free period for more than 8-10 years is predicted. EFFECT: use of the declared method allows determining the early onset of metastases or recurrence after combinatory treatment. 3 ex
The invention relates to medicine. More specifically to Oncology. And can be used in the prognosis of the disease cervical cancer among women of reproductive age after the combined treatment. There is a method of individual forecasting resetstatistics survival in patients with cervical cancer (cervical cancer) on set obtained at the end of treatment indicators such as the level of hemoglobin, the absolute number of lymphocytes, the content of large granulometries lymphocytes, figure lymphocytic-macrophage transformation of monocytes, relationships, activity of superoxide dismutase and total peroxidase activity in lymphocytes and neutrophils, the relationship of vitamin E to vitamin a in neutrophils, the content of vitamin E in erythrocytes to plasma ("Conservative treatment of cervical cancer stage III with endolymphatic introduction of immunomodulatory drugs" Rozenko L.Y., author. Diss. Prof. the honey. Sciences. Rostov n/A., 1999). But the proposed method for predicting relapse-free and resetstatistics survival of cervical cancer patients contains too many indicators that are not directly connected to each other, the result is difficult to know which ones are more informative, and which are only an addition to the main one. The combined analysis of all parameters requires not only m the CSOs of time to perform them, but a large number of performers. Due to its complexity, bulkiness, lack of definition it cannot be used to predict the time of occurrence of metastasis of cervical cancer. There is a method of forecasting extracranial metastases in patients with metastases to the brain by the magnitude of the content of cortisol in the blood of a patient with brain damage. With a significant reduction of hormone levels on the physiological norm note the presence of metastases in other organs (patent NG 2300110. Bull. No. 15 from 27.05.2007 "Method of detection of extracranial metastases in cancer patients with metastatic brain") The authors suggest as a criterion for predicting metastasis to use cortisol levels. However, cortisol has no direct effect on proliferative processes in the cervix. From the interaction of estrogens and progestins depends proanagen or antioncogene situation in the reproductive organs. During radical surgery with tumor removed and the ovaries; in the body creates a new hormonal situation and new relationships in hormone levels between the two classes of sex hormones in terms of proliferative processes: estrogens and progestins. There is a need to find new criteria for determining d is italmost resetstatistics period. The aim of the invention is the determination criterion of the appearance of metastases. The goal is to reach what 10-14 days after radical resection of malignant tumors of the cervix in daily urine of the patient to determine the number of sex hormones estradiol and pregnandiol. Next, calculate the ratio of the first to the second, with the value of more than 0.8 predicting the occurrence of metastasis or recurrence of the disease in the next year or two, and if the value is less than 0.8, the duration resetstatistics period of more than 8-10 years. Analysis of the known methods of determining the timing of occurrence of metastasis or recurrence of the disease and developed allows to establish the existence of novelty and difference of the latter. The novelty of the claimed "Method for predicting metastasis of cervical cancer" is that as a criterion, the time of occurrence of metastasis or recurrence of the disease made the ratio of sex hormones estradiol and pregnandiol. After radical surgical removal of the tumor, these sex hormones directly reflect both the state of sexual status and the condition of the whole organism and oncogenic resistance in particular. In the available sources of information Russia, CIS and foreign countries of a similar method of determining the timing of occurrence of metastases or p is of sidebow was not found. Developed a Method for predicting metastasis of cervical cancer is industrially applicable. It can be reproduced and repeated many times in gynecologic hospitals General medical and specialized hospitals of oncorine. The method is as follows. After specification of the diagnosis and the implementation of radical removal of the tumor formation at 10-14 day gather during the day the urine of the patient and determine the biochemical standard methods sex hormones: estradiol and pregnandiol. Next, calculate the ratio of estradiol to pregnandiol. If the value is more than 0.8 predicting the occurrence of metastasis or recurrence of the disease in the next year or two, and when the ratio of less than 0.8 - resistant resetstatistics or disease-free period of more than 8-10 years. An example is the following extract from the records. 1. Patient C, 1969, history 20330/kg, was admitted to the Department of gynecology №1 RNII in October 2007 with a diagnosis of cervical cancer St W, with complaints to contact spotting from the genital tract. 10.10.07 the operation of Wertheim. Histological analysis 972940-55 usernotification squamous cell carcinoma with invasion 6 mm 14 days after surgery in daily urine the patient has identified the hormones estradiol and pregnan the IOL. The content in daily urine estradiol 2,8, pregnandiol 2,3. Calculated the ratio of estradiol to pregnandiol, it amounted to 1.21. With 23.10.07 on 6.12.07 held DHT on the pelvic organs in SOD 40 Gy, intracavitary Co60 therapy in SOD 18 Gr. 22.09.09 due to low back pain are assigned SRCT the study of the lumbar and sacral spine. Revealed metastatic lesion paraaortal lymph nodes, the side surface of the body of the second lumbar vertebra, the iliopsoas muscles. One year and eleven months after the combined treatment (radical surgical removal of the tumor, the patient was identified metastases in lymph nodes and the lateral surface of the vertebral body. In further held DHT on paraaortal lymph nodes and the 2nd lumbar vertebra in SOD 40 Gy and 3-year autohemotherapy. Heading dose - cyclophosphamide 600 mg, cisplatin 150 mg, doxorubicin 30 mg 2. A female patient, 1956, history 4536/l, was admitted to the Department of gynecology №1 RNII in April 2000 with a diagnosis of cervical cancer St IB, complaints contact spotting for 2 months. 18.04.2000, the operation of Wertheim. Histological analysis 565268-82 usernotification squamous cell carcinoma with keratinization, the invasion more than 1 cm lymph node metastasis no. the via 14 days after surgery, daily urine the patient has identified the hormones estradiol and pregnandiol. The content in daily urine estradiol 4,1, pregnandiol of 7.3. Calculated the ratio of estradiol to pregnandiol, he was 0,56. In the postoperative period held DHT on the pelvic organs in SOD 40 Gy and intracavitary Co60 therapy in SOD 30 Gr. 12.02.2009 year examination in the clinic, RNII data for recurrence and metastases are not detected. 3. Patient A-C, born in 1968, history 15950/l, was admitted to the Department of gynecology №1 RNII in December 2000, a diagnosis of cervical cancer St Ia, complaints at admission was not charged. 7.12.2000, the design Bureau No. 2 performed electroconsult cervical regarding the proposed endocervicosis, postoperative histological analysis No. 41425 - 31 squamous cell carcinoma, invasion 3 mm. 26.12.2000, the operation of Wertheim, histological analysis 590241 - 48 focal dysplasia of the cervical epithelium II-III degree, granulation tissue, endocervicosis. Lymph node metastases not. After 10 days of operation daily urine the patient has identified the hormones estradiol and pregnandiol. The content in daily urine estradiol 1,6, pregnandiol of 2.5. Calculated the ratio of estradiol to pregnandiol, he made 0.64. In the postoperative period held DHT on the pelvic organs in SOD 40 Gr. 31.08.2009 year examination in the clinic, RNII data for recurrence and metastasis is e identified. The proposed method of determining the timing of occurrence of metastasis or recurrence of the disease was carried out a study of the ratio of estradiol to pregnandiol in 27 patients with cervical cancer. Women with the ratio of estradiol to pregnandiol was less than 0.8, no metastasis or recurrence of the disease for 8-10 years after radical resection of malignant tumors of the cervix. Technical and economic efficiency of the proposed "Method for predicting metastasis of cervical cancer is possible: - for each patient to determine the early stages of the emergence of metastasis or recurrence of the disease; - to conduct an individualized treatment based on the status of the patient; - to determine the ratio of hormones without significant material costs. A method for predicting metastasis of cervical cancer, including radical surgical removal of tumor formation and biochemical studies, characterized in that 10-14 days after surgical removal of the tumor, the patient in daily urine to determine the number of sex hormones: estradiol and pregnandiol; then calculate the ratio of the first to the second, and if the value is more than 0.8 predicting the occurrence of metastasis or recurrence of the disease in the next year or two, and when the ratio IU the its forecast of 0.8 resetstatistics, relapse-free period of more than 8-10 years.
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