Nutrient milk medium for preparation of bacteria-probiotics biomass |
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IPC classes for russian patent Nutrient milk medium for preparation of bacteria-probiotics biomass (RU 2326939):
Consortium of lactobacteria strains and method of receiving preparation on its basis, which is used as dietary supplement or ferment for production of sour milk products / 2326938
Consortium of lactobacteria strains contains Lactobacillus acidophilus 57S ("ВКПМ В-5863"), Lactobacillus piantarum "П-75" ("ВКПМ В-3962"), Lactobacillus casei stilb ("ВКПМ В-5724"), which are taken in proportion of 2:0.5:0.5. The method includes preparation of milk nutrient medium, its sterilisation and cooling down to the temperature of fermentation, introduction of inoculation dose of lactobacteria strains consortium in the quantity of not more than 5.0 mass %. The product is fermented during time, which is sufficient for formation of clot and the ready product is cooled at the temperature of not more than +6°C. As milk nutrient medium non-fat milk is used, normalised in solid residual down to 13.5-15%, lactopeptone and lithium citrate with the following proportion of components, (mass %): lactopeptone - 3.5-4.5, lithium citrate - 0.15-0.25 and non-fat milk, normalised in solid residual down to 13.5-15% - the rest.
 Antiphage nutritional broth for activating starter populations / 2326163
The invention may be used for producing dairy products with high quality factors. A nutritional broth comprising skimmed milk, gum arabic, lithium citrate, D-glucosamine, L-rhamnose, D-arabinose, tap water.
Bacteria strain lactococcus lactis subsp diacetilactis ТК-ОБЛ-Д5-5Ф, used for revealing lactococci bacteriophages / 2324733
Invention relates to diary industry, to production of fermented milk products in particular. Bacteria strain Lactococcus lactis subsp. diacetilactis "ТК-ОБЛ-Д5-5Ф" (Russian collection of lactate bacteria for production of cheese and bacteriophages for them at the "ВНИИМС") was obtained from self-sour cream and selected by the sensitivity spectrum for 250 known bacteriophages. It is used as an indicator strain for revealing the lactococci bacteriophages, ways and sources of their spreading at an enterprise. The invention enables one to prevent development of bacteriophages in production of milk produce, to improve its quality and safety for human health, as well as to increase efficiency of the production.
Process of nutrient medium production / 2324732
Lactoserum containing 10% of dry substances will be pasteurised at 90°C during 20 min, cooled to 45°C, reduced with ammonia solution to pH 7.0. Then the Streptococcus thermophilus barm will be introduced and the mixture will be ripened at 40°C during 5 hours, additive will be introduced in the form of milk protein hydrolysate, the mixture will be mixed and sterilised, cooled to 38°C and reduced with ammonia solution to pH 6.8. The nutrient medium thus obtained will enable one to shorten the process of cultivating bifido- and lactobacteria to 24 hours and to increase their concentration when obtaining liquid bacterial concentrate.
 Biocatalist on base of immobilize cells of photosynethic bacteria for producing hydrogen / 2323975
Biocatalyst is made on the base of immobilized cells of photosynthetic bacteria, includes to the matrix of criogel polyvenial alcohol, thanks to which it is produced the hydrogen production. Biocatalist has long time of service, obtain seriously modified productivity and can be used for hydrogen producing in reactors of different types.
Nutrient medium for securing streptococci / 2323969
Nutrient medium contains the pancreatic casein hydrolysate, enzymatic peptone, activator of hemophilic microorganisms' growth, bread yeast, lactose, bromocresol purple, glucose, L-cysteine, sodium sulfite, sodium citrate, sodium azide, crystal violet, agar-agar, distilled water.
Brevibacillus laterosporus bacterium strain inhibiting and preventing development of microphytic algae of various taxonomic types / 2323968
Brevibacillus laterosporus "ВКПМ" В-9405 bacterium strain is separated by means of multistage selection from the natural Brevibacillus laterosporus "ВКПМ" В-8287 strain. The algicide activity is estimated by the strain lytic action on microalgae defining residual optical density (OD). OD is 10.1%. The strain algistatic activity is estimated by defining optical density. The optical density is 1.950.
 Preparation for soil cleaning from arsenic and protection of plants from diseases inhibited by plant pathogenic fungi and preudomonas aureofaciens arc v-2390 d bacterium strain for its making / 2323967
Preudomonas aureofaciens KR31 (pKS1) strain separated from the rhizosphere of alfalfa in Krasnodar Territory and deposited to the All-Russian collection ARC V-2390 D, is capable of inhibiting growth of a wide range of plant pathogenic fungi, stimulates plat growth, resistant to arsenic, dissolves phosphates. Preparation containing Preudomonas aureofaciens bacterium strain cells suspension may be used to clean the soil from arsenic and to protect the plants from the diseases, caused by plant pathogenic fungi.
Bioactive organomineral slow-release fertilizer / 2323918
Organomineral fertilizer, which contains rock phosphate (apatite), natural zeolite, oxidised brown coal in 1:0.15:2-1:0.2:5 ratio, is composted for 30-60 days, while exposed to phosphate-assimilating bacteria in amount of 105-106 cells/spores/ml, isolated from local soil using selection method. Content of labile phosphorus increases from 17.0 to 50.4 mg/kg of soil, and phosphatase activity in chestnut soil increases by 2.8-21.8 times.
Method for preparing halophilic bacterium biomass / 2323251
Method involves culturing halophilic microorganisms in the presence of encapsulated adsorbent and/or antioxidant up to the stationary growth phase in preparing the seeding material. Then in submerged culturing fresh medium is added by portions in the amount 1/3 of the final volume of cultural fluid followed by contacting cultural fluid with an encapsulated adsorbent and/or antioxidant, and additional feeding with dry or concentrated medium is carried out. The amount of dissolved oxygen is maintained at the level 5-10% of the equilibrium level. Invention provides increasing the level of accumulation of biomass, to enhance the specific content of bacteriorhodopsin in halophilic microorganisms biomass and the total yield of bacteriorhodopsin at the minimal content of carotinoids in biomass, and to reduce consumption of nutrient medium also.
Consortium of lactobacteria strains and method of receiving preparation on its basis, which is used as dietary supplement or ferment for production of sour milk products / 2326938
Consortium of lactobacteria strains contains Lactobacillus acidophilus 57S ("ВКПМ В-5863"), Lactobacillus piantarum "П-75" ("ВКПМ В-3962"), Lactobacillus casei stilb ("ВКПМ В-5724"), which are taken in proportion of 2:0.5:0.5. The method includes preparation of milk nutrient medium, its sterilisation and cooling down to the temperature of fermentation, introduction of inoculation dose of lactobacteria strains consortium in the quantity of not more than 5.0 mass %. The product is fermented during time, which is sufficient for formation of clot and the ready product is cooled at the temperature of not more than +6°C. As milk nutrient medium non-fat milk is used, normalised in solid residual down to 13.5-15%, lactopeptone and lithium citrate with the following proportion of components, (mass %): lactopeptone - 3.5-4.5, lithium citrate - 0.15-0.25 and non-fat milk, normalised in solid residual down to 13.5-15% - the rest.
 Production method for foodstuff (variants) / 2323586
Method consists in: mixing the preparation of viable probiotic microorganisms with additional ingredients, drying till the water activity less than 0.3, mixture pressing under the pressure to receive granules with the volume at least 0.02 cm³, coating with the water-proof protective coat and simultaneously keeping the water less than 0.3 and introduction of granules into liquid, humid and semi-humid food product. The pressing stage may be before drying.
Wound-healing biopreparation as gel / 2322996
Invention relates to producing and using immunobiological preparations comprising Bacillus subtilis biomass. Biopreparation represents a wound-healing gel comprising biomass of antagonistically active strain of Bacillus subtilis 3H or Bacillus subtilis 11B and special additives additionally. Invention provides effective treatment of inflammatory processes on skin and mucosa. Invention can be used in dermatology, gynecology and surgery for topical using.
Immunobiological agent possessing ability for stimulation of human psychoemotional adaptation (variants), and bifidobacterium bifidum 1c strain / 2322252
Invention proposes agents (variants) possessing ability for stimulation of human psychoemotional adaptation. Agent comprises a dry strain of Bifidobacterium bifidum 1C deposited in the State collection of microorganisms representing microorganisms of the normal human microflora - FGUN MNIIEM named for G.N.Gabrichevsky at number 208 and taken in the amount 106 - 1010 CFU (colony-forming units), and pharmaceutically acceptable special additives taken in the amount 30.0-94.0 wt.-% of the agent mass. The proposed immunobiological agent can be represented as dry biomass, powder, tablet being among them as a tablet with an enteric-soluble cover, and capsule being among capsule with an enteric-soluble cover. The strain Bifidobacterium bifidum 1C is prepared by the successive multiple passages through the nutrient medium BS and selection of clones based on the presence and stabilization of a feature for stimulation of the human psychoemotional adaptation. Invention provides enhancing effectiveness of stimulation of human psychoemotional adaptation.
 Method for complex correction of intestinal dysbiosis with the help of liquid probiotic "narine-forte" and prebiotic syrup "phytolon" with chlorophyll / 2322251
The present innovation deals with correcting intestinal dysbiosis due to the intake of probiotic and prebiotic products either within the scope of prophylactic procedures or in complex therapy. The innovation refers to combined peroral intake of liquid concentrated symbiotic lactic acid product of microbial origin "NARINE-FORTE" and Syrup "PHYTOLON" with chlorophyll which should be applied during meals or after meals. The innovation normalizes and protects human intestinal microflora against harmful impact of inadequate nutrition, contaminated environment, stress situations, infections, aftereffects of therapy with antibiotics and other chemopreparations, radiation therapy and other negative actions.
 Lypoteichoic acid from lactic acid bacteria and uses thereof in modulation of immune reactions mediated by gram-negative bacteria and potential pathogenic gram-positive bacteria / 2320356
Invention relates to composition for inhibition or prevention of inflammatory processes associated with bacterium-mediated diseases or LTA/LPS-mediated disturbances in gastrointestinal tract in human or animal. Claimed composition contains as active ingredient lypoteichoic acid from lactic acid bacteria belonging to genus Lactobacillus wherein lypoteichoic acid was selected on the base of its capability of binding to CD 14 and disability of inducing the release of anti-inflammatory cytokines such as IL-8 or TNF-α from enteric epithelial cells. Disclosed is application of at least one lypoteichoic acid from lactic acid bacteria belonging to genus Lactobacillus, and/or lactic acid bacterium producing the same, and/or supernatant of its culture for composition production useful in inhibition or prevention of inflammatory processes associated with bacterium-mediated diseases or LTA/LPS-mediated disturbances in gastrointestinal tract, bones, skin, eyes, ears, lungs, and oral cavity in human or animal.
Method for production of biomass from liquid lactobacterium autostrain / 2320355
Claimed method includes sampling of native material, sample seeding on selective nutrient medium without dilution not later than 2 h after sampling; lactobacterium growing and pure culture isolation. Repeated passage is carried out. Obtained pure lactobacterium culture is grown in selective liquid medium followed by addition of conserving agent such as sucrose-gelatin medium in ratio of 1:1 and controlling of obtained liquid biomass.
 Microorganisms as carriers of nucleotide sequences encoding cellular antigens for treatment of tumors / 2319741
Invention relates to genetically modified microorganism comprising the following components in its genomes that are expressed: (I) nucleotide sequence encoding at least one the tumor cell antigen epitope, and/or nucleotide sequence of at least one antigen epitope showing specificity to tissue cell generating tumor; (II) nucleotide sequence encoding protein that stimulates cell of the immune system; (IIIA) nucleotide sequence of the transport system providing expression of product of components (I) and (II) expression on external surface of microorganism, and/or secretion of product of component (I) and component (II) expression, and/or (IIIB) nucleotide sequence of protein for lysis of microorganism in mammal cells cytosol and for intracellular releasing plasmas containing in microorganisms to be lysed, and (IV) activating sequence activated in microorganism showing specificity to tissue cell but not specific to cell for expression of one or some components from (I) to (IIIB), and wherein each of components from (I) to (IV) can be represented once or many times. Proposed microorganism is useful for using as a drug as component of anti-tumor vaccine. Also, invention describes plasmid or the expression vector for preparing the genetically modified microorganism and a method for its preparing. Vaccine based on genetically modified microorganism represents the improved and overcome immune tolerance with respect to tumors in case for its using for prophylaxis and therapy of tumors.
Method for preventing puerperal complications in cows / 2319493
It is necessary to introduce culture liquid of bacterial strain "Bacillus subtilis TNP-3-DEP" at the dosage of 2 ml thrice at a 10-d-long interval. The innovation normalizes vaginal microbiocenosis and increases immunobiological reactivity.
Strain streptococcus vestibularis slk-92 used in preparing probiotic preparation designated for prophylaxis and treatment of bacterial intestine infection in agricultural young stock animals / 2317328
Strain Streptococcus vestibularis SLK-92 is isolated from calf corpse intestine and deposited in All-Russian State Collection of strains of microorganisms at FGU "All-Union State center for quality and standardization of medicinal agents for animals and fodders", FGU "VGNKI" - 08.12.39. Using the proposed strain in newborn calves and piglets for prophylaxis and treatment of bacterial intestine infections provides reducing their morbidity, to decrease lethality of animals and multiplicity feeding the preparation to agricultural young stock animals. Invention can be used in preparing probiotic preparation possessing prophylactic and curative effect in intestinal infections in agricultural young stock animals caused by pathogenic enterobacteria of genus Escherichia, Proteus, Morganella, Citrobacter, Enterobacter, Klebsiella and Providencia.
 Strain of lactobacilli lactobacillus paracasei cncm i-2116 (ncc 2461) eliciting ability to prevent intestine colonization with pathogenic microorganisms causing diarrhea, supernatant of its culture and oral agent for prophylaxis and/or treatment of diarrhea-associated disorders / 2243779
Invention relates to the strain Lactobacillus paracasei CNCM I-2116 used for diarrhea prophylaxis causing by pathogenic microorganisms. Supernatant of this strain culture elicits ability to prevent colonization of intestine with pathogenic microorganisms causing diarrhea also and this strain is designated for preparing agent used for prophylaxis and/or treatment of disorders associated with diarrhea. Agent for oral administration represents therapeutically effective dose of the strain L. paracasei CNCM I-2116 or supernatant of its culture and acceptable foodstuff. Invention provides the enhanced viability of the strain in its applying and effectiveness in prophylaxis of adhesion to intestine cells and invasion to intestine cells of pathogenic microorganisms causing diarrhea.
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FIELD: technological processes. SUBSTANCE: invention is related to preparation of nutrient mediums for cultivation of bifido and lactobacteria with the purpose of preparation of microorganisms biomass and its further utilisation in production of ferments for medicinal and prophylactic products, and also for production of dietary supplements (DS). Milk nutrient medium contains the basis in the form of non-fat milk with content of solid residual 13-14%, muriatic cysteine or ascorbic acid, lactopeptone, purified yeast extract with index of amine nitrogen 2.1-2.8%, total protein of at least 3-4%, sodium chloride, manganese sulphate heptahydrate and manganese sulphate tetrahydrate. EFFECT: provides for the possibility of bifido and lactobacteria cultivation with longer shelf life and with higher titres of bacteria biomass in the process of storage. 2 cl, 4 tbl, 5 ex
The invention relates to biotechnology and can be used in microbiological, food industry and for the preparation of nutrient media for cultivation of bifidobacteria and lactobacilli with the aim of obtaining biomass of microorganisms and its further use in the production of starter cultures for therapeutic products, as well as for production of biologically active additives (BAA). Currently, the food and dietary Supplement with probiotic microorganisms occupy a leading place in the complex of measures aimed at the prevention and correction of disorders of the microflora. The importance of probiotic preparations and products requires the development of new efficient low-cost production of culture media for producing microbial mass. In the manufacture of dietary supplements with probiotics, as well as therapeutic products and starter cultures is important for them is to develop a nutritional basis for probiotic microorganisms. Such a framework should include all the basic components that match the needs of the bacteria. Cultivation in this environment should contribute at least the preservation of probiotic properties of microorganisms. Production nutrient medium should provide a high speed reproduction: the varnish is of bacteri up to 5-7 hours and bifidobacteria to 18 hours, a high concentration of viable microbial cells in a unit volume of the medium is not less than 1·1010CFU/ml, the high conservation of viable microbial cells - at least 70-90 days. Nutritional requirements of lactic acid bacteria are very diverse and are associated with biochemical activity of microorganisms. As enrichment medium protein increases the ability of bacteria to brivati lactose, adding Peptones and peptides (products of protein hydrolysis) enhances the growth of lactic acid bacteria to a much greater extent than amino acids. It should be noted that the number of amino acids required by various lactic acid bacteria, is variable and depends on the needs of the strains. The bifidobacteria are the most required amino acids: Proline, serine, alanine, aspartic and glutamic acid. Having some proteolytic activity, bifidobacteria can to a small extent to additionally provide for their needs in nitrogenous substances. All the nutrients in the environment must be in easily digestible form, proteins, in particular, in the form of hydrolysates, autolysates. In some cases imperfect technology of obtaining protein hydrolysates and autolysates can cause them significant amounts of the peptides of high molecular weight, ammonia, and OCD is Chenin impurities, formed due to the decomposition of labile amino acids and interaction with aldehydes. The presence of these components in the environment significantly reduces nutrient efficiency. The quality of protein hydrolysates largely due to the chosen method of carrying out hydrolysis providing the desired degree of hydrolysis and degree of subsequent treatment of the resulting hydrolysate. For increasing biomass use nutrient media of different composition. Known composition of the nutrient medium for the growth of bifido - and lactobacteria, representing a hydrolysate of milk, which is introduced as additives peptone, sodium chloride, agar-agar, lactose, cysteine hydrochloric acid, in the following ratio of components (instructions for the preparation of fermented milk bifidumbakterin at milk kitchens. - M.: RSFSR Ministry of health, 1987, - 11 C.), g/l:
This composition finds application in practice for the growth of bifido - and lactobacteria. However, along with the road base - hydrolysis of the volume of milk it contains and expensive supplements such as peptone, cysteine hydrochloric acid. In addition, this composition is not sufficiently effective nutrient medium for cultivation of bifidobacteria and lactobacilli. Known nutrient medium for growing the biomass of bifidobacteria and lactobacilli-based hydrolysate of pea in the following ratio of components (RF Patent No. 2061037, IPC 12N 1/20, publ. 27.05.96 g), wt.%:
The composition has a low content of low molecular weight peptides and amino acids (Amino nitrogen 130-150 mg%), therefore, does not provide a time of high biomass concentration is not more than 108CFU/ml At the same time, the increase in microbial mass (not less than 24 hours), titratable acidity and terms of preservation do not meet the requirements. Also known nutrient medium for the accumulation of biomass of bacteria on the basis of whey in the following ratio of components (RF Patent No. 2087532, IPC 12N 1/20, publ. 20.08.97 g), wt%:
Medium of similar composition, but gives a high rate of growth, does not have enough nutrients (proteins, peptides, amino acids) for long-term preservation of microbial cells, which is very important for drugs and dietary SUPPLEMENTS-probiotics. An additional effect of high growth parameters is a significant increase in titratable acidity, negatively affecting the organoleptic properties of the final product. The presence of expensive components (agar-agar, cysteine) in the production of drugs and SUPPLEMENTS-probiotics increases the cost of the final product. Widely known corn-lactose medium (CCL) for the cultivation of bifidobacteria and lactic acid bacteria (TU 10-02-02-789-192-95):
However, CCL does not contain hydrolyzed protein components of milk or yeast cells, which does not ensure the cultivation of long term safety of the final product. The presence in the environment of non-standard component - corn extract causes the irregularity of the medium, as well as unsatisfactory organoleptic properties of the resulting BAD and sourdough. The closest analogue (prototype) to declare milk is a nutrient medium to obtain a liquid concentrate of bifidobacteria containing the basis cysteine hydrochloric acid (L-cystine), D(-)-lactose, hydrolyzed milk proteins and autolysate yeast and sodium chloride. As a hydrolysate of milk proteins using pancreatic hydrolysate of milk products milk, as autolysate yeast using enzymatic autolysate yeast, and is used as a base normals is consistent on the dry rest skim milk milk when the total content of amino nitrogen in the nutrient medium 260-300 mg% in the following ratio of its components, wt.% (patent RF №2169763, IPC C12N 1/20, AK 35/74, AS 9/12, publ. 27.06.2001,):
As enzymatic autolysate yeast use autolysate with the indicator amino nitrogen 350-400 mg%. Milk products milk normalized to the dry residue to the value of 13.0-14%. In addition, the culture medium was additionally make soy protein isolate in an amount by weight of 0.2-0.3%. To activate soy protein isolate in nutrient medium further added sodium citrate in an amount by weight of 0.1-0.2%. However, pancreatic hydrolysate of proteins of milk products milk contains, along with high and low molecular weight peptides partially hydrolyzed casein and other components, which are poorly digested by the bacteria and enzymatic autolysate yeast along with vitamins and stimulating growth factors bacteria contain toxic in the society - the products of enzymatic autolysis, which do not favor the growth of bacteria (Themeroller, Avhub and Lpositive. Effect of enzymatic hydrolysates on the accumulation of biomass of bifidobacteria. Journe. "The dairy industry", No. 12, 1980, - p.15-17), which would reduce the time and title of the biomass of bifidobacteria and lactobacilli in storage. The technical result of the claimed invention is to provide such a medium, which would provide the possibility of cultivation as bifidobacteria and lactobacilli for longer storage times and higher titers biomass of bacteria during storage. This technical result is achieved by the fact that the dairy nutrient medium to obtain the biomass of bacteria-probiotics containing Foundation, normalized to the dry residue of skim milk milk, cysteine hydrochloric acid (L-cystine) or ascorbic acid, hydrolyzed milk proteins and growth factors from yeast cells, sodium chloride, according to the invention, the medium further comprises manganese sulfate MnSO4·4H2O and magnesium sulfate MgSO4·7H2Oh, as a hydrolysate of milk proteins using enzymatic hydrolysate of whey proteins of milk, as growth factors from yeast cells using the untreated yeast extract in the following ratio of its components, wt.%:
Dairy nutrient medium contains purified yeast extract with the indicator amino nitrogen 2,1-2,8%, total protein not less than 3-4%, enzymatic hydrolyzed whey proteins of milk (lactate) with the index of total protein not less than 11%, the amine nitrogen of not less than 4%, and skim milk milk contains lactose in an amount not exceeding 15%, and normalized to the dry residue to the value 13,0-14,0%. The total amine nitrogen in the nutrient medium contains not less than 300 mg%. Introduction to the basis environment enzymatic hydrolysate (bactopeptone) whey proteins milk is (lactalbumin and lactoglobulin) provides enriching it with a mixture of high molecular weight and low molecular weight peptides, which are more effective stimulants of growth of bifidobacteria and lactobacilli than, for example, pancreatic hydrolysate of proteins (the main component - casein) milk skim milk. Whey milk proteins by amino acid composition similar to proteins of breast milk. In comparison with casein they are more easily subjected to complete hydrolysis, contain essential sulfur-containing acid in an amount corresponding to the human needs 4 times more contain cysteine and 19 times more tryptophan and their use is preferred in the production of probiotics for children. Purified extract of yeast contains b vitamins and stimulating factors in the growth of bifidobacteria, as well as the optimal content of amino nitrogen, ensuring the most favorable conditions for the growth of bifidobacteria and lactobacilli compared with enzymatic autolysate yeast does not contain toxic products of yeast metabolism, inhibit the growth of bacteria. Noticeable improvement of the quality yeast extract is achieved by improving the production technology is translated into a soluble state 30-50% yeast protein, increasing the digestibility of cell protein by 8-10% by loosening all of the layers of the cell wall. Yeast extract contains: essential amino acids - lysine, met the onin war, glycine, alanine, histidine, aspartic acid, threonine, glutamic acid, valine, and others; - vitamins - Riboflavin (b2), thiamine (1), pyridoxine (B6), ciankobalamin (12), Niacin (PP); - protective peptides. Components, providing the reducing properties of the inventive environment are mainly cysteine or ascorbic acid, and amino acids glutamine and asparagine, which are rich in purified yeast extract, which is part of the environment. To ensure the growth and development of lactic acid bacteria need in inorganic compounds, especially in manganese ions and magnesium ions. Manganese prevents autolysis of cells and is necessary for normal processes of fat metabolism. Mn++and Mg++remove toxic effect of Zn++if present in the environment. The invention allows to obtain a high initial titer and to increase the shelf life without oppression growth and an increase in active acidity of probiotic preparations obtained using the proposed nutrient medium. The lack of ready nitrogenous substances in the inventive culture medium can be recovered by adding skim milk bactopeptone (enzymatically hydrolyzed whey proteins of milk and yeast extract. Whey milk proteins play an important role in increasing bio is assy probiotic bacteria and the formation of the quality of the final product, because they have a high content of essential and sulfur-containing amino acids. Proteins dairy base (mainly casein) are characterized by high biological value and a unique set of amino acids that helps improve overall balance of amino acid composition of the final product. Milk contains a unique disaccharide is lactose, which has a beneficial effect on the composition normoflora intestine. When heating milk, for example during sterilization, lactose is transformed into lactulose, which is a powerful bifidogenic factor. Milk contains a significant amount of macro - and micronutrients, vitamins. Especially important digestible milk calcium, vitamins A, B1In2, D, C, carotene. The following are examples of compositions of the claimed nutrient medium. Example 1. The minimum content of the components (wt.%):
Example 2. The average content of components (wt.%):
Example 3. The maximum content of components (wt.%):
The following data (tables 1 and 2) for the cultivation of bifidobacteria (Bifidobacterium bifidum 791 BUG) and lactobacilli (strain Lactobacillus Lactobacillus acidophilus 57S) using the claimed nutrient medium and environment of the prototype. From these tables it is seen that the titer as bifidobacteria and lactobacilli after cultivation with the use of the claimed nutrient medium several times higher than with the use environment of the prototype, and the cultivation correspondingly less.
Example 4. Study of the retention of the consortium of strains of lactic acid bacteria in a liquid concentrate of Lactobacillus Investigated the duration of storage of the consortium of strains of lactic acid bacteria in a liquid concentrate (table 3). As the sowing of the dose used, the consortium of strains of lactobacilli Lactobacillus acidophilus 57S (deposited in Vniigenetika in PMBC under No 5863), Lactobacillus plantarum P-75 (deposited in Vniigenetika in PMBC under No 3962); Lactobacillus casei SS (deposited in Vniigenetika in PMBC under No 5724) in the ratio 2:0,5:0,5. The fermentation is carried out at a temperature (39-40)°C for 4-5 hours before reaching the acidity of the finished product is not less than 100°So A considerable time (up to 72 days) storage titer not less than 10 CFU/ml is associated with providing the initial acidity of the drug consortium of lactobacilli (120-140)°T, obtaining the original title of lactobacilli (not below 1010CFU/ml) by using the proposed dairy nutrient medium with the addition of bactopeptone and yeast extract.
Example 5. Study of the retention of biomass consortium of strains of bifidobacteria in the liquid concentrate of bifidobacteria obtained using the claimed nutrient medium Researched length is lnost storage consortium of strains of bifidobacteria in the liquid concentrate of bifidobacteria (table 4). As sowing dose of bifidobacteria using a consortium of strains of bacteria: Bifidobacterium bifidum 8-3, Bifidobacterium longum I-3, Bifidobacterium adolescentis B-1 in a ratio of 3:1:1. Sowing dose of bifidobacteria contribute in the amount of 5.0-7.0% of the volume of the nutrient medium with the title of bifidobacteria not less than 109CFU/ml, culturing is carried out for a time sufficient for obtaining a concentrate of bifidobacteria with the biological titer of not less than (0,8-1,0)1011CFU/ml A considerable time (up to 72 days) storage of liquid concentrate of bifidobacteria with a titer of not less than 1010CFU/ml is associated with providing the initial acidity of the drug consortium of bifidobacteria (140-145)°T, obtaining the original title of bifidobacteria (not below 1011CFU/ml).
Thus, the technical result of the claimed invention confirms the creation of such a nourishing environment that provides opportunities for cultivation as bifidobacteria and lactobacilli for longer term storage (up to 72 hours or more) and higher titers biomass of bacteria during storage. 1. Dairy nutrient medium to obtain the biomass of bacteria-probiotics-containing base in the form of skim milk with a dry matter content of 13-14%, cysteine hydrochloric acid or ascorbic acid, hydrolyzed milk proteins, growth facto the s from yeast cells and sodium chloride, wherein the environment further comprises manganese sulfuric acid chetyrehpolnye and magnesium sulfuric acid as, as hydrolysate of milk proteins contains bactopeptone, and as growth factors from yeast cells purified yeast extract with the indicator amino nitrogen 2,1-2,8%, total protein not less than 3-4% in the following ratio of components of the nutrient medium, wt.%:
purified yeast extract with
2. Dairy nutrient medium according to claim 1, characterized in that skim milk contains varnish the RAM in an amount of not more than 15%.
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