Bacteria strain lactococcus lactis subsp diacetilactis ТК-ОБЛ-Д5-5Ф, used for revealing lactococci bacteriophages |
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IPC classes for russian patent Bacteria strain lactococcus lactis subsp diacetilactis ТК-ОБЛ-Д5-5Ф, used for revealing lactococci bacteriophages (RU 2324733):
Process of nutrient medium production / 2324732
Lactoserum containing 10% of dry substances will be pasteurised at 90°C during 20 min, cooled to 45°C, reduced with ammonia solution to pH 7.0. Then the Streptococcus thermophilus barm will be introduced and the mixture will be ripened at 40°C during 5 hours, additive will be introduced in the form of milk protein hydrolysate, the mixture will be mixed and sterilised, cooled to 38°C and reduced with ammonia solution to pH 6.8. The nutrient medium thus obtained will enable one to shorten the process of cultivating bifido- and lactobacteria to 24 hours and to increase their concentration when obtaining liquid bacterial concentrate.
 Biocatalist on base of immobilize cells of photosynethic bacteria for producing hydrogen / 2323975
Biocatalyst is made on the base of immobilized cells of photosynthetic bacteria, includes to the matrix of criogel polyvenial alcohol, thanks to which it is produced the hydrogen production. Biocatalist has long time of service, obtain seriously modified productivity and can be used for hydrogen producing in reactors of different types.
Nutrient medium for securing streptococci / 2323969
Nutrient medium contains the pancreatic casein hydrolysate, enzymatic peptone, activator of hemophilic microorganisms' growth, bread yeast, lactose, bromocresol purple, glucose, L-cysteine, sodium sulfite, sodium citrate, sodium azide, crystal violet, agar-agar, distilled water.
Brevibacillus laterosporus bacterium strain inhibiting and preventing development of microphytic algae of various taxonomic types / 2323968
Brevibacillus laterosporus "ВКПМ" В-9405 bacterium strain is separated by means of multistage selection from the natural Brevibacillus laterosporus "ВКПМ" В-8287 strain. The algicide activity is estimated by the strain lytic action on microalgae defining residual optical density (OD). OD is 10.1%. The strain algistatic activity is estimated by defining optical density. The optical density is 1.950.
 Preparation for soil cleaning from arsenic and protection of plants from diseases inhibited by plant pathogenic fungi and preudomonas aureofaciens arc v-2390 d bacterium strain for its making / 2323967
Preudomonas aureofaciens KR31 (pKS1) strain separated from the rhizosphere of alfalfa in Krasnodar Territory and deposited to the All-Russian collection ARC V-2390 D, is capable of inhibiting growth of a wide range of plant pathogenic fungi, stimulates plat growth, resistant to arsenic, dissolves phosphates. Preparation containing Preudomonas aureofaciens bacterium strain cells suspension may be used to clean the soil from arsenic and to protect the plants from the diseases, caused by plant pathogenic fungi.
Bioactive organomineral slow-release fertilizer / 2323918
Organomineral fertilizer, which contains rock phosphate (apatite), natural zeolite, oxidised brown coal in 1:0.15:2-1:0.2:5 ratio, is composted for 30-60 days, while exposed to phosphate-assimilating bacteria in amount of 105-106 cells/spores/ml, isolated from local soil using selection method. Content of labile phosphorus increases from 17.0 to 50.4 mg/kg of soil, and phosphatase activity in chestnut soil increases by 2.8-21.8 times.
Method for preparing halophilic bacterium biomass / 2323251
Method involves culturing halophilic microorganisms in the presence of encapsulated adsorbent and/or antioxidant up to the stationary growth phase in preparing the seeding material. Then in submerged culturing fresh medium is added by portions in the amount 1/3 of the final volume of cultural fluid followed by contacting cultural fluid with an encapsulated adsorbent and/or antioxidant, and additional feeding with dry or concentrated medium is carried out. The amount of dissolved oxygen is maintained at the level 5-10% of the equilibrium level. Invention provides increasing the level of accumulation of biomass, to enhance the specific content of bacteriorhodopsin in halophilic microorganisms biomass and the total yield of bacteriorhodopsin at the minimal content of carotinoids in biomass, and to reduce consumption of nutrient medium also.
Method for preparing halophilic bacterium biomass / 2323226
Method involves using an encapsulated adsorbent and/or antioxidant in preparing seeding material and submerged culturing. In preparing seeding material the culturing process is carried out in freshly prepared nutrient medium up to the stationary growth phase. Submerged culturing is carried out by simultaneous inoculation of bioreactor with seeding material and contacting the content of bioreactor with encapsulated adsorbent and/or antioxidant. The oxygen content in bioreactor is maintained at the level 5-10% of the equilibrium level. Invention provides increasing yield of halophilic microorganisms biomass up to 42 g/l and with the content of bacteriorhodopsin up to 1.6 g/l and in practically absent of carotinoids in biomass. Invention provides reducing time for preparing the unit mass of the end product and to decrease consumptions for production of bacteriorhodopsin.
Nutrient medium for culturing mycobacterium and nocardioformous actinomyces / 2322495
Invention proposes a nutrient medium comprising potassium hydrogen phosphate, magnesium sulfate, L-asparagine, glycerol, citric acid, ferrous ammonium citrate, agar, humivite and distilled water. Invention provides enhancing growth properties of the nutrient medium.
 Microorganism strain bacillus simplex as producer of site-specific endonuclease blsi / 2322494
Invention proposes the strain Bacillus simplex 23 isolated from soil and providing preparing site-specific endonuclease. This enzyme is able for recognizing and cleaving both chain in nucleotide sequence of DNA comprising at least one C5-methylcytosine base in the recognition site 5'-GCNGC-3' to form 3'-prominent ends. The novel strain can be used for isolation of the novel site-specific endonuclease that can be used for detection and cleavage of methylated sites in DNA.
 Strain of lactobacilli lactobacillus paracasei cncm i-2116 (ncc 2461) eliciting ability to prevent intestine colonization with pathogenic microorganisms causing diarrhea, supernatant of its culture and oral agent for prophylaxis and/or treatment of diarrhea-associated disorders / 2243779
Invention relates to the strain Lactobacillus paracasei CNCM I-2116 used for diarrhea prophylaxis causing by pathogenic microorganisms. Supernatant of this strain culture elicits ability to prevent colonization of intestine with pathogenic microorganisms causing diarrhea also and this strain is designated for preparing agent used for prophylaxis and/or treatment of disorders associated with diarrhea. Agent for oral administration represents therapeutically effective dose of the strain L. paracasei CNCM I-2116 or supernatant of its culture and acceptable foodstuff. Invention provides the enhanced viability of the strain in its applying and effectiveness in prophylaxis of adhesion to intestine cells and invasion to intestine cells of pathogenic microorganisms causing diarrhea.
Strain of microorganism lactobacillus buchneri 66 for ensilage of maize green mass and preserving flattened grain / 2243999
The strain Lactobacillus buchneri 600 is isolated from barley grains at milkwax stage of ripeness. The strain Lactobacillus buchneri 600 is registered in 05. 08. 2002 year in the All-Russian State collection of microorganism strains used in veterinary science and animal husbandry at number Lactobacillus buchneri VGNKI 02.08.04-DEP and deposited in the collection 000 "Biotrof". Invention provides the more effective multiplication of the strain in maize ensilaging green mass and preserving flattened grains with enhanced formation of lactic acid, inhibition of putrid microflora that allows preparing fodder from vegetable raw with enhanced quality. Invention can be used in fodder production for ensilage maize green mass and preserving flattened grains.
Strain lactobacillus acidophilus as producer of fodder protein / 2244000
Invention relates to a new isolated strain of Lactobacillus acidophilus 1660/08 as a producer of the protein fodder. The strain Lactobacillus acidophilus 1660/08 is obtained by the selection method and selected by its ability to form significant amount of crude protein and to accumulate the biomass. The strain is deposited in the VGNKI collection at number VGNKI-03.04.10.-DEP. Invention provides eliminating the environment pollution in producing the protein fodder, to enhance the specific protein yield, to reduce energy consumptions in preparing protein fodder, to simplify and to accelerate the process in its preparing, to simplify equipment fitting out and to utilize waste in manufactures using natural raw.
Strain lactobacillus plantarum as producer of fodder protein / 2244001
The strain Lactobacillus plantarum 578/25 is obtained by method of step-by-step selection and selected by its ability to produce significant amount of crude protein and to accumulate the biomass. The strain is deposited in the VGNKI collection at number VGNKI-03.04.09.-DEP. Invention provides eliminating the pollution of environment in producing the protein fodder, to elevate the protein specific yield, to reduce energy consumptions in preparing protein fodder, to simplify and to accelerate the process of its preparing, to simplify apparatus equipment, to utilize waste in manufacturing using the natural raw.
 Strain of microorganism lactobacillus lactis for preparing curd from milk / 2244002
The strain of microorganism Lactobacillus lactis VKPM B-8354 is prepared without using mutagens and genetic methods and shows resistance against broad spectrum of lactophages. The strain ferments effectively milk from different trading sorts, with broad range of fatness and different methods of thermal treatment. Individual specific properties of the strain allows its applying as a monostrain ferment. Curd obtained with applying the strain L. lactis VKPM B-8354 shows good organoleptic qualities, nonacid taste and homogenous consistence. The strain is suitable especially for plants with small volume of manufacture but with varied assortment.
Method for isolation and selection of microorganisms as producers of cyclodextrin glucanotrasnferase, strain of microorganism bacillus circulans b-65 ncaim (p) 001277 (b-65) as producer of extracellular cyclodextrin transferase, cyclodextrin glucanotransferase obtained from its and its applying for preparing cyclodextrin / 2244742
The strain Bacillus circulans B-65 a producer of cyclodextrin glucanotransferase is isolated and selected form the soil sample by culturing in nutrient medium with amylolytic activity 12.17 U/ml and cyclodextrinogenic activity up to 0.530 U/ml. Cyclodextrin glucanotransferase isolated from B. circulans B-65 shows the high degree for conversion of starch to cyclodextrins and this enzyme is specific for formation of β-cyclodextrin. Invention can be used in food industry for preparing cyclodextrins and cyclodextrin glucanotransferase used in different branches of industry.
Bacterial preparation, method for its producing, nutrient medium for culturing cells escherichia coli vkm cr-322d and method for prophylaxis and treatment of gastroenteric disease in agricultural and domestic animal and poultry / 2244743
For preparing a preparation cells of microorganism Escherichia coli VKM CR-322D is cultured in nutrient medium containing Hottinger's broth, glucose, yeast extract, manganese sulfate, potassium hydrophosphate, sodium chloride and tap water in the content of amine nitrogen 125-155 mg%. Glucose is added by batch portions in the process of culturing cells that is carried out at temperature 30-31oC at stirring and aeration for 10-12 h. Prepared cultural fluid containing 3 x 109 bacterial cells/ml is mixed with protective sucrose-gelatin medium and subjected for lyophilic drying. Dried mass is stored under nitrogen that enhances safety of viable cells in the preparation. Applying the preparation for prophylaxis and treatment of agricultural and domestic animals and poultries with gastroenteric diseases provides its effectiveness.
Method for preparing liquid lactobacterin / 2244744
Method for preparing liquid lactobacterin involves regeneration, culturing passages of lyophilized culture and culturing ferment of lactobacilli in liquid lyophilized nutrient medium containing dry defatted milk enzymatic hydrolyzate with the content of amine nitrogen 1 485 mg%, 30.0 ± 3.0 g/l; yeast concentrated autolyzate, 110.0 ± 10 g/l; food agar, 0.8 g/l, and distilled water, up to 1 l. Culturing ferment is carried out up to accumulation of biomass of lactobacilli 109-1010 CFU/ml. Then 10-30% of supernatant liquid is removed from the ready product and replaced it with equal volume of fresh nutrient medium. Invention provides simplifying technology in preparing liquid lactobacterin and to elevate the storage period of viable lactobacilli. Invention can be used in producing probiotic preparations.
Strain bifidobacterium longum 379-in used for preparing bacterial preparations, biologically active supplements for food, ferments, fermented-dairy and nonfermented dairy foodstuffs, hygienic and cosmetic agents / 2244745
The strain Bifidobacterium longum 379-IN is obtained by selection without using methods of genetic modification of the strain Bifidobacterium longum B379M and distinct by ability to utilize insulin. The strain is deposited in GKNM GU "MNIIEM named for G. N. Gabrichevskiy Russia Ministry of Public Health" at № 172. The strain shows high technological effectiveness, accumulates biomass with substrates of vegetable origin and artificial nutrient media for short periods with concentration of bifidobacteria, it elicits acid-forming and antagonistic properties with respect to pathogenic and putrid microflora. This allows its using in manufacturing bacterial preparations, biologically active supplements for food, fermented-dairy and nonfermented-dairy foodstuffs, ferments, hygienic and cosmetic agents providing probiotic effect and normalization of microbiocenosis in human body, among them in gastroenteric and urogenital tracts, cutaneous and mucosa integuments. Invention can be used in manufacturing bacterial preparations, biologically active supplements for food, fermented-dairy and nonfermented-dairy foodstuffs, hygienic and cosmetic agents.
Nutrient medium for culturing plague microorganism vaccine strain / 2245362
Invention relates, in particular, to preparing nutrient media used for culturing the plague microorganism vaccine strain and can be used in medicinal microbiology. The nutrient medium for culturing the plague microorganism vaccine strain comprises additionally as a stimulating additive sodium sulfite and as a nutrient base - soybean fruits enzymatic hydrolyzate in the following ratio of components, g/l: microbiological agar, 11.0-13.0; soybean fruits enzymatic hydrolyzate, 250.0-350.0; sodium chloride, 4.5-5.5; sodium hydrogen phosphate, 3.5-4.5; sodium sulfite, 0.0003-0.0005; distilled water, the balance. Invention provides enhancing the growth property of nutrient medium.
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FIELD: chemistry, organic, production of fermented milk products. SUBSTANCE: invention relates to diary industry, to production of fermented milk products in particular. Bacteria strain Lactococcus lactis subsp. diacetilactis "ТК-ОБЛ-Д5-5Ф" (Russian collection of lactate bacteria for production of cheese and bacteriophages for them at the "ВНИИМС") was obtained from self-sour cream and selected by the sensitivity spectrum for 250 known bacteriophages. It is used as an indicator strain for revealing the lactococci bacteriophages, ways and sources of their spreading at an enterprise. The invention enables one to prevent development of bacteriophages in production of milk produce, to improve its quality and safety for human health, as well as to increase efficiency of the production. EFFECT: increase of the production efficiency, quality and safety of milk produce. 1 ex
The invention relates to the dairy industry, in particular the production of fermented dairy products, and represents a new strain of bacteria of the lactococcal, which can be widely used for the detection of bacteriophages in dairy enterprises. One of the conditions for obtaining high-quality dairy products is to prevent the development of bacteriophages in production. For this purpose it is necessary to use a set of indicator crops that are sensitive to a wide range of bacteriophages. Known strains of bacteria of the lactococcal used for the detection of bacteriophages (USSR author's certificate No. 605833, CL SC 1/02, AS 9/12, 1978; USSR author's certificate No. 555133, CL SC 1/02, AS 9/12, 1975). They differ in the set lyse their phages (pagation). However, the known strains have relatively low sensitivity to bacteriophages. Closest to the proposed (selected as a prototype) is a strain of Streptococcus lactis No. 3938-Srused for the detection of bacteriophages of lactic streptococci (Copyright certificate №605833, CL SC 1/02, AS 9/12, 1978). The disadvantage of this strain is that it is sensitive to the relatively small number of bacteriophages that was previously allocated to dairy enterprises. At present range of bacteriophages on Moloch the x plants has changed and expanded. To identify them we need new strains that are sensitive to the bacteriophage with a modified spectrum of political action. The challenge is to prevent the development of bacteriophages in dairy products by their identification with the help of indicator crops. The technical result is the identification of a new strain of Lactococcus lactis subsp. diacetilactis TC-REG-D5-5F, sensitive to a wide range of bacteriophages circulating on dairy enterprises. Bacterial strain Lactococcus lactis subsp. diacetilactis TC-REG-D5-5F obtained from samokatnoy sour cream and selected range of sensitivity to 250 bacteriophages allocated to dairy enterprises of the CIS for more than 20 years. Bacterial strain Lactococcus lactis subsp. diacetilactis TC-REG-D5-5F different from known strains of lactococcal set lytic phages (pagation), sensitive to 44% of lactococcal bacteriophages (110 out of 250 studied). Bacterial strain Lactococcus lactis subsp. diacetilactis TC-REG-D5-5F is stored in the Russian collection of lactic acid bacteria for the production of cheeses and bacteriophages to them when VNIIMS, enabled the government of the Russian Federation of 24 June 1996, No. 725-47 in the list of collections, depositing for the state needs microorganisms, cultured cells of higher plants, transplantable somatic cells of vertebrates. Bacterial strain Lactococcus lactis subsp. diacetlactis TC-REG-D5-5F characterized by the following features. Morphological and cultural characteristics. Cell population are gram-positive cocci with a diameter of 0.8 to 0.9 μm, arranged in pairs and in short chains (3-4 cells). On the surface of the agar with hydrolyzed milk forms a teardrop-shaped whitish shiny surface of the colony and logotherapie deep colonies. On agar with hydrolyzed milk and calcium citrate around colonies observed zone of enlightenment. When grown in broth from hydrolyzed milk forms a uniform dense suspension. Skim milk roll at 30°With a dose of inoculum of 0.1% for 16-18 h, when adding 5% inoculum for 5-7 hours the Number of viable cells in culture in the non-fatty milk 108-109CFU/DM3. Physiological and biochemical properties. Optional gone anaerobic. The optimal growth temperature (30±2)°C, the pH of the medium 6.8 or 6.9. Not produce catalase, gas from glucose. Ferments glucose, galactose, lactose, mannitol, dextrin, maltose, sucrose, arabinose. Not ferments sorbitol, raffinose, inulin, glycerin. Disposes of citrate in the presence of carbohydrates from carbon dioxide, diacetyl + acetone. Forms ammonia from arginine. Not growing in litmus milk at 45°and when the concentration of the salt of 6.5% at 30°C. Has a typical the properties, characteristic of Lactococcus lactis subsp. diacetilactis. Storage conditions. The strain is stored in lyophilized form at a temperature of 6-8°C. Environment drying - skimmed milk. Sensitivity to bacteriophages. The strain is sensitive to 110 virulent bacteriophages from 250 studied allocated to dairy enterprises of the CIS. Example Identification of lactococcal bacteriophages using a strain of Lactococcus lactis subsp. diacetilactis TC-REG-D5-5F. 1. Preparation of lawns strain In Petri dishes pour 15-20 ml of 1.5% agar with hydrolyzed milk and dried for 3-4 hours Pre-poured in a test tube, 2.5 ml of 0.6% agar with hydrolyzed milk melted at a temperature of 100°C, cooled to 45°With, make 1 ml of 18-hour broth culture of Lactococcus lactis subsp. diacetilactis TC-REG-D5-5F, mixed and poured on Petri dishes with nutrient dense environment. 2. Preparation of test samples. Analyzed for the presence of bacteriophage samples (milk, yeast, whey, etc.) pre-released from the protein by the action of lactic acid), filtered through cotton-gauze, then bacterial (pore size 0.2 µm) filters. 3. Detection of bacteriophages. On the surface of the double-layer agar seeded culture put drops investigated bacteriophage samples, cups incubated at 30°18-24 hours a Strain of Lactococcus lactis subsp. iacetilactis TC-REG-D5-5F forms on the surface of the Cup uniform muddy lawn. If the application site drops are formed of a transparent zone, then in the test sample is a bacteriophage, a lytic strain of Lactococcus lactis subsp. diacetilactis TC-REG-D5-5F. Thus, the identified new indicator strain Lactococcus lactis subsp. diacetilactis TC-REG-D5-5F, sensitive to a wide range of lactococcal bacteriophages. The proposed strain of bacteria is recommended for wide use in the dairy industry for the identification of lactococcal bacteriophages, routes and sources of their distribution in the enterprise. This will help to prevent the development of bacteriophages in the production of fermented dairy products, improve their quality and safety for the health of the consumer, and increase production efficiency. Bacterial strain Lactococcus lactis subsp. diacetilactis Russian collection of lactic acid bacteria for the production of cheeses and bacteriophages to them when VNIIMS, reg. No. of TC-REG-D5-5F were used for detection of lactococcal bacteriophages.
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