Process of nutrient medium production |
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IPC classes for russian patent Process of nutrient medium production (RU 2324732):
 Biocatalist on base of immobilize cells of photosynethic bacteria for producing hydrogen / 2323975
Biocatalyst is made on the base of immobilized cells of photosynthetic bacteria, includes to the matrix of criogel polyvenial alcohol, thanks to which it is produced the hydrogen production. Biocatalist has long time of service, obtain seriously modified productivity and can be used for hydrogen producing in reactors of different types.
Nutrient medium for securing streptococci / 2323969
Nutrient medium contains the pancreatic casein hydrolysate, enzymatic peptone, activator of hemophilic microorganisms' growth, bread yeast, lactose, bromocresol purple, glucose, L-cysteine, sodium sulfite, sodium citrate, sodium azide, crystal violet, agar-agar, distilled water.
Brevibacillus laterosporus bacterium strain inhibiting and preventing development of microphytic algae of various taxonomic types / 2323968
Brevibacillus laterosporus "ВКПМ" В-9405 bacterium strain is separated by means of multistage selection from the natural Brevibacillus laterosporus "ВКПМ" В-8287 strain. The algicide activity is estimated by the strain lytic action on microalgae defining residual optical density (OD). OD is 10.1%. The strain algistatic activity is estimated by defining optical density. The optical density is 1.950.
 Preparation for soil cleaning from arsenic and protection of plants from diseases inhibited by plant pathogenic fungi and preudomonas aureofaciens arc v-2390 d bacterium strain for its making / 2323967
Preudomonas aureofaciens KR31 (pKS1) strain separated from the rhizosphere of alfalfa in Krasnodar Territory and deposited to the All-Russian collection ARC V-2390 D, is capable of inhibiting growth of a wide range of plant pathogenic fungi, stimulates plat growth, resistant to arsenic, dissolves phosphates. Preparation containing Preudomonas aureofaciens bacterium strain cells suspension may be used to clean the soil from arsenic and to protect the plants from the diseases, caused by plant pathogenic fungi.
Bioactive organomineral slow-release fertilizer / 2323918
Organomineral fertilizer, which contains rock phosphate (apatite), natural zeolite, oxidised brown coal in 1:0.15:2-1:0.2:5 ratio, is composted for 30-60 days, while exposed to phosphate-assimilating bacteria in amount of 105-106 cells/spores/ml, isolated from local soil using selection method. Content of labile phosphorus increases from 17.0 to 50.4 mg/kg of soil, and phosphatase activity in chestnut soil increases by 2.8-21.8 times.
Method for preparing halophilic bacterium biomass / 2323251
Method involves culturing halophilic microorganisms in the presence of encapsulated adsorbent and/or antioxidant up to the stationary growth phase in preparing the seeding material. Then in submerged culturing fresh medium is added by portions in the amount 1/3 of the final volume of cultural fluid followed by contacting cultural fluid with an encapsulated adsorbent and/or antioxidant, and additional feeding with dry or concentrated medium is carried out. The amount of dissolved oxygen is maintained at the level 5-10% of the equilibrium level. Invention provides increasing the level of accumulation of biomass, to enhance the specific content of bacteriorhodopsin in halophilic microorganisms biomass and the total yield of bacteriorhodopsin at the minimal content of carotinoids in biomass, and to reduce consumption of nutrient medium also.
Method for preparing halophilic bacterium biomass / 2323226
Method involves using an encapsulated adsorbent and/or antioxidant in preparing seeding material and submerged culturing. In preparing seeding material the culturing process is carried out in freshly prepared nutrient medium up to the stationary growth phase. Submerged culturing is carried out by simultaneous inoculation of bioreactor with seeding material and contacting the content of bioreactor with encapsulated adsorbent and/or antioxidant. The oxygen content in bioreactor is maintained at the level 5-10% of the equilibrium level. Invention provides increasing yield of halophilic microorganisms biomass up to 42 g/l and with the content of bacteriorhodopsin up to 1.6 g/l and in practically absent of carotinoids in biomass. Invention provides reducing time for preparing the unit mass of the end product and to decrease consumptions for production of bacteriorhodopsin.
Nutrient medium for culturing mycobacterium and nocardioformous actinomyces / 2322495
Invention proposes a nutrient medium comprising potassium hydrogen phosphate, magnesium sulfate, L-asparagine, glycerol, citric acid, ferrous ammonium citrate, agar, humivite and distilled water. Invention provides enhancing growth properties of the nutrient medium.
 Microorganism strain bacillus simplex as producer of site-specific endonuclease blsi / 2322494
Invention proposes the strain Bacillus simplex 23 isolated from soil and providing preparing site-specific endonuclease. This enzyme is able for recognizing and cleaving both chain in nucleotide sequence of DNA comprising at least one C5-methylcytosine base in the recognition site 5'-GCNGC-3' to form 3'-prominent ends. The novel strain can be used for isolation of the novel site-specific endonuclease that can be used for detection and cleavage of methylated sites in DNA.
Microorganism strain paracoccus denitrificans as producer of exopolysaccharide and exopolysaccharide / 2322493
Invention relates to a novel culture of microorganism producing high-molecular exopolysaccharide. Invention proposes the strain of microorganism Paracoccus denitrificans VKPM B-8617 that produces exopolysaccharide possessing cross-linking properties in aqueous and water-containing hydrocarbon systems. Exopolysaccharide is formed by residues of glucose, galactose, mannose and rhamnose in the ratio = 52:4:1, respectively, and comprises glucuronic and pyruvic acids, and acyl groups also and has molecular mass (0.5 x 106)-(2 x 107) Da. This exopolysaccharide is able to form pseudoplastic and thixotropic highly viscous solutions showing stable values of dynamic viscosity in the range of temperature from 20°C to 90°C and unstratifying emulsions. Proposed exopolysaccharide can be used in building, paper, textile, perfume-cosmetic, food, chemical, oil- and gas-extracting industry, agriculture, and in pharmaceutics and medicine.
 Strain of lactobacilli lactobacillus paracasei cncm i-2116 (ncc 2461) eliciting ability to prevent intestine colonization with pathogenic microorganisms causing diarrhea, supernatant of its culture and oral agent for prophylaxis and/or treatment of diarrhea-associated disorders / 2243779
Invention relates to the strain Lactobacillus paracasei CNCM I-2116 used for diarrhea prophylaxis causing by pathogenic microorganisms. Supernatant of this strain culture elicits ability to prevent colonization of intestine with pathogenic microorganisms causing diarrhea also and this strain is designated for preparing agent used for prophylaxis and/or treatment of disorders associated with diarrhea. Agent for oral administration represents therapeutically effective dose of the strain L. paracasei CNCM I-2116 or supernatant of its culture and acceptable foodstuff. Invention provides the enhanced viability of the strain in its applying and effectiveness in prophylaxis of adhesion to intestine cells and invasion to intestine cells of pathogenic microorganisms causing diarrhea.
Strain of microorganism lactobacillus buchneri 66 for ensilage of maize green mass and preserving flattened grain / 2243999
The strain Lactobacillus buchneri 600 is isolated from barley grains at milkwax stage of ripeness. The strain Lactobacillus buchneri 600 is registered in 05. 08. 2002 year in the All-Russian State collection of microorganism strains used in veterinary science and animal husbandry at number Lactobacillus buchneri VGNKI 02.08.04-DEP and deposited in the collection 000 "Biotrof". Invention provides the more effective multiplication of the strain in maize ensilaging green mass and preserving flattened grains with enhanced formation of lactic acid, inhibition of putrid microflora that allows preparing fodder from vegetable raw with enhanced quality. Invention can be used in fodder production for ensilage maize green mass and preserving flattened grains.
Strain lactobacillus acidophilus as producer of fodder protein / 2244000
Invention relates to a new isolated strain of Lactobacillus acidophilus 1660/08 as a producer of the protein fodder. The strain Lactobacillus acidophilus 1660/08 is obtained by the selection method and selected by its ability to form significant amount of crude protein and to accumulate the biomass. The strain is deposited in the VGNKI collection at number VGNKI-03.04.10.-DEP. Invention provides eliminating the environment pollution in producing the protein fodder, to enhance the specific protein yield, to reduce energy consumptions in preparing protein fodder, to simplify and to accelerate the process in its preparing, to simplify equipment fitting out and to utilize waste in manufactures using natural raw.
Strain lactobacillus plantarum as producer of fodder protein / 2244001
The strain Lactobacillus plantarum 578/25 is obtained by method of step-by-step selection and selected by its ability to produce significant amount of crude protein and to accumulate the biomass. The strain is deposited in the VGNKI collection at number VGNKI-03.04.09.-DEP. Invention provides eliminating the pollution of environment in producing the protein fodder, to elevate the protein specific yield, to reduce energy consumptions in preparing protein fodder, to simplify and to accelerate the process of its preparing, to simplify apparatus equipment, to utilize waste in manufacturing using the natural raw.
 Strain of microorganism lactobacillus lactis for preparing curd from milk / 2244002
The strain of microorganism Lactobacillus lactis VKPM B-8354 is prepared without using mutagens and genetic methods and shows resistance against broad spectrum of lactophages. The strain ferments effectively milk from different trading sorts, with broad range of fatness and different methods of thermal treatment. Individual specific properties of the strain allows its applying as a monostrain ferment. Curd obtained with applying the strain L. lactis VKPM B-8354 shows good organoleptic qualities, nonacid taste and homogenous consistence. The strain is suitable especially for plants with small volume of manufacture but with varied assortment.
Method for isolation and selection of microorganisms as producers of cyclodextrin glucanotrasnferase, strain of microorganism bacillus circulans b-65 ncaim (p) 001277 (b-65) as producer of extracellular cyclodextrin transferase, cyclodextrin glucanotransferase obtained from its and its applying for preparing cyclodextrin / 2244742
The strain Bacillus circulans B-65 a producer of cyclodextrin glucanotransferase is isolated and selected form the soil sample by culturing in nutrient medium with amylolytic activity 12.17 U/ml and cyclodextrinogenic activity up to 0.530 U/ml. Cyclodextrin glucanotransferase isolated from B. circulans B-65 shows the high degree for conversion of starch to cyclodextrins and this enzyme is specific for formation of β-cyclodextrin. Invention can be used in food industry for preparing cyclodextrins and cyclodextrin glucanotransferase used in different branches of industry.
Bacterial preparation, method for its producing, nutrient medium for culturing cells escherichia coli vkm cr-322d and method for prophylaxis and treatment of gastroenteric disease in agricultural and domestic animal and poultry / 2244743
For preparing a preparation cells of microorganism Escherichia coli VKM CR-322D is cultured in nutrient medium containing Hottinger's broth, glucose, yeast extract, manganese sulfate, potassium hydrophosphate, sodium chloride and tap water in the content of amine nitrogen 125-155 mg%. Glucose is added by batch portions in the process of culturing cells that is carried out at temperature 30-31oC at stirring and aeration for 10-12 h. Prepared cultural fluid containing 3 x 109 bacterial cells/ml is mixed with protective sucrose-gelatin medium and subjected for lyophilic drying. Dried mass is stored under nitrogen that enhances safety of viable cells in the preparation. Applying the preparation for prophylaxis and treatment of agricultural and domestic animals and poultries with gastroenteric diseases provides its effectiveness.
Method for preparing liquid lactobacterin / 2244744
Method for preparing liquid lactobacterin involves regeneration, culturing passages of lyophilized culture and culturing ferment of lactobacilli in liquid lyophilized nutrient medium containing dry defatted milk enzymatic hydrolyzate with the content of amine nitrogen 1 485 mg%, 30.0 ± 3.0 g/l; yeast concentrated autolyzate, 110.0 ± 10 g/l; food agar, 0.8 g/l, and distilled water, up to 1 l. Culturing ferment is carried out up to accumulation of biomass of lactobacilli 109-1010 CFU/ml. Then 10-30% of supernatant liquid is removed from the ready product and replaced it with equal volume of fresh nutrient medium. Invention provides simplifying technology in preparing liquid lactobacterin and to elevate the storage period of viable lactobacilli. Invention can be used in producing probiotic preparations.
Strain bifidobacterium longum 379-in used for preparing bacterial preparations, biologically active supplements for food, ferments, fermented-dairy and nonfermented dairy foodstuffs, hygienic and cosmetic agents / 2244745
The strain Bifidobacterium longum 379-IN is obtained by selection without using methods of genetic modification of the strain Bifidobacterium longum B379M and distinct by ability to utilize insulin. The strain is deposited in GKNM GU "MNIIEM named for G. N. Gabrichevskiy Russia Ministry of Public Health" at № 172. The strain shows high technological effectiveness, accumulates biomass with substrates of vegetable origin and artificial nutrient media for short periods with concentration of bifidobacteria, it elicits acid-forming and antagonistic properties with respect to pathogenic and putrid microflora. This allows its using in manufacturing bacterial preparations, biologically active supplements for food, fermented-dairy and nonfermented-dairy foodstuffs, ferments, hygienic and cosmetic agents providing probiotic effect and normalization of microbiocenosis in human body, among them in gastroenteric and urogenital tracts, cutaneous and mucosa integuments. Invention can be used in manufacturing bacterial preparations, biologically active supplements for food, fermented-dairy and nonfermented-dairy foodstuffs, hygienic and cosmetic agents.
Nutrient medium for culturing plague microorganism vaccine strain / 2245362
Invention relates, in particular, to preparing nutrient media used for culturing the plague microorganism vaccine strain and can be used in medicinal microbiology. The nutrient medium for culturing the plague microorganism vaccine strain comprises additionally as a stimulating additive sodium sulfite and as a nutrient base - soybean fruits enzymatic hydrolyzate in the following ratio of components, g/l: microbiological agar, 11.0-13.0; soybean fruits enzymatic hydrolyzate, 250.0-350.0; sodium chloride, 4.5-5.5; sodium hydrogen phosphate, 3.5-4.5; sodium sulfite, 0.0003-0.0005; distilled water, the balance. Invention provides enhancing the growth property of nutrient medium.
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FIELD: chemistry, organic, production of sour milk products. SUBSTANCE: lactoserum containing 10% of dry substances will be pasteurised at 90°C during 20 min, cooled to 45°C, reduced with ammonia solution to pH 7.0. Then the Streptococcus thermophilus barm will be introduced and the mixture will be ripened at 40°C during 5 hours, additive will be introduced in the form of milk protein hydrolysate, the mixture will be mixed and sterilised, cooled to 38°C and reduced with ammonia solution to pH 6.8. The nutrient medium thus obtained will enable one to shorten the process of cultivating bifido- and lactobacteria to 24 hours and to increase their concentration when obtaining liquid bacterial concentrate. EFFECT: shortening of cultivating bacteria and increase in their concentration when obtaining liquid bacterial concentrate. 3 ex
The invention relates to the dairy industry, namely the production method of nutrient medium, preferably hydrolysate-milk used in the production of bacterial concentrates bifidobacteria and lactobacilli. Numerous hydrolysate-dairy nutrient medium on the basis of skim milk or whey, and their hydrolysates containing milk proteins (including hydrolyzed), lactose, mineral salts and various additives, which improve the growth of lactic acid microorganisms. To further improve the growth properties of the mediums they are additional supplements (growth promoters)that enhances the growth of microorganisms. Closest to the proposed method is a method of preparation of nutrient media, including the introduction in the original culture medium additive of the hydrolysate of milk protein (casein-whey mass), mixing, deoxidizing solution of ammonia, sterilization, cooling to a temperature of cultivation of bifidobacteria. The original culture medium served as an aqueous solution, g/l: sodium chloride (5,0), agar-agar (0.75 in), lactose (10), ascorbic acid (1,0), FeSO4(0,002) (abstract of Diss. SOIC. academic Art. candles. the honey. of Sciences, Rostov-on-don, 1995). However, this method and culture medium prepared in this way is, subsequent use is not effective to improve the growth of microorganisms and does not provide liquid bacterial concentrate with high concentrations of bifidobacteria and lactobacilli, as well as increase the optimal duration of cultivation of microorganisms to 48 hours. The technical result of the invention to provide a nourishing environment for subsequent use to produce liquid bacterial concentrate with a higher content of microorganisms, as well as reducing the duration of the process of their cultivation up to 20-24 hours. The technical result is achieved in that the method comprising introducing an additive hydrolysate of milk protein in the original growth medium, mixing the deoxidizing solution of ammonia, sterilization and cooling, according to the invention as a source of nutrient medium use whey containing 6-15% of dry matter, before making the additive source nutrient medium pasteurized at 90-95°C for 10-30 min, cooled to 40-45°, rascist to a pH of 6.8 to 7.0, make a starter of Streptococcus thermophilus, the mixture sours at 40-45°C for 3-6 hours, and then deoxidizing solution of ammonia is carried out after cooling the sterilized nutrient medium. redlagaemyi method is implemented as follows. The original culture medium (whey, containing 6-15% of dry matter), placed in a cultivator, pasteurized at 90-95°C for 10-30 min, cooled to 40-45°, rascist ammonia solution to a pH of 6.8 to 7.0, contribute 3-5% of the leaven of the Str. thermophilus and sours at 40-45°C for 3-6 hours, make a hydrolyzed milk protein, mixed, sterilized at 116-120°C for 30-60 minutes, cooled and rascist ammonia solution to a pH of 6.8 to 7.0. The obtained culture medium is used as a nutrient medium for cultivation of lactic acid bacteria or bifidobacteria in the production of bacterial concentrates. The proposed method in the process of ripening the source of the nutrient medium growing biomass Str. thermophilus synthesizes and secretes in skvashivaemoe Wednesday β-galactosidase is an enzyme that converts lactose in the mixture of glucose and galactose, which accumulate in skvashivaemoe environment and during subsequent use of the nutrient medium, obtained by the proposed method can be easily assimilated by microorganisms, improving and accelerating their growth. When sterilization fermented mixture of biomass Str. thermophilus turns into bacterial autolysate, also accelerating the growth of microorganisms during subsequent use of the obtained medium. Loss of glucose and galactose in the sterilization minimum in sour is (not demanding) environment and significantly increase with pre-sterilization demanding environment. Therefore, the proposed method of deoxidizing environment is carried out after sterilization and cooling, because it increases the safety contained in the environment of glucose and galactose in the sterilization process. Example 1. 600 kg whey, containing 15% solids, is placed in a cultivator, pasteurized at 95°C for 10 minutes, cooled to 45°, rascist ammonia solution to a pH of 6.8, contribute 5% of the leaven of the Str. thermophilus and sours mix at 40°C for 3 hours, make 250 kg of hydrolyzed skim milk containing 12% solids, mixed, sterilized mixture 116°C for 60 minutes, cooled cooked medium to a temperature of subsequent use (38° (C) and rascist ammonia solution to a pH of 6.8 to 7.0. Example 2. 400 kg whey, containing 6% solids is placed in a cultivator, pasteurized at 90°C for 30 minutes, cooled to 40°, rascist ammonia solution to pH 7.0, contribute 3% of the leaven of the Str. thermophilus and sours mix at 45°C for 6 hours, making 400 kg of hydrolyzed skim milk containing 9% solids, mixed, sterilized mixture at 120°C for 30 minutes, cooled cooked medium to a temperature of subsequent use and rascist ammonia solution to a pH of 6.8 to 7.0. Example 3. 700 kg of milk is whey, containing 10% dry substance, is placed in a cultivator, pasteurized at 90°C for 20 minutes, cooled to 45°, rascist ammonia solution to pH 7.0, contributed 4% of the leaven of the Str. thermophilus and sours mix at 40°C for 5 hours, make 200 kg of hydrolyzed milk protein, containing 10% solids, mixed, sterilized mixture 116°C for 30 minutes, cooled to 38°and rascist ammonia solution to a pH of 6.8. The production method of the nutrient medium, including the introduction of additives hydrolysate of milk protein in the original growth medium, mixing the deoxidizing solution of ammonia, sterilization and cooling, characterized in that as the source of the nutrient medium use whey containing 6-15% of dry matter, before making the additive source nutrient medium pasteurized at 90-95°C for 10-30 min, cooled to 40-45°, rascist to a pH of 6.8 to 7.0, make a starter of Streptococcus thermophilus and sours at 40-45°C for 3-6 h, and the deoxidation of the nutrient medium solution of ammonia is carried out after cooling the sterilized nutrient medium.
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