Nutrient medium for securing streptococci |
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IPC classes for russian patent Nutrient medium for securing streptococci (RU 2323969):
Method for bacteriological detection of nocardiomorphous actinomyces / 2321636
Method involves preparing pair samples from the pathological material sample. The first sample is poured with 0.5%, and the second sample - with 2.0% aqueous solution of the preparation "Leseptik" taken in the volume ratio 1:2 followed by their exposition for 3 h and 15 min, respectively, at room temperature. Then the "Leseptik" preparation solution is poured off, washed out twice with isotonic sodium chloride solution, and suspension is prepared that is inoculated on solid egg-containing media and grown at temperature +37°C. Invention provides accelerated primary indication and differentiation of positive reactions for PPD-tuberculin for mammals caused by mycobacterium tuberculosis from positive reactions for PPD-tuberculin for mammals caused by corynebacteria, rhodococci and nontuberculosis mycobacteria.
 Method for selection of petroleum-oxidizing microorganisms as producers of biosurfactants / 2320715
Method involves culturing petroleum-oxidizing microorganisms on solid medium in Petri dishes at room temperature. In termination of culturing morphotype of microorganism colonies is determined. Microorganism colonies of M-type are selected as producers of biosurfactants. Microorganism colonies of R- and S-type are washed out with phosphate buffer, pH 6.8, and a prepared suspension is diluted to optical value density 0.1-0.3 at wavelength 670 nm followed by assay of emulsifying activity and index of cell hydrophobicity. Microorganisms as producers of biosurfactants are selected at hydrophobicity index value above 20% and the positive emulsifying activity. Method is simple in realization and allows carrying out selection of petroleum-oxidizing microorganisms as producers of extracellular and cell-bound biosurfactants.
Method for detection of candida albicans by biorhythms / 2319747
Invention relates to a method for microbiological laboratory identification of fungi of Candida genus. Detection of Candida albicans is carried out by biorhythms of fungus. Analyzed culture of fungi is cultured for 24 h and then suspension of fungi is applied on the surface of solid nutrient medium in the concentration 300 CFU/ml in the amount 0.1 ml, and amount of grown colonies is determined for 24 h with a break for 4 h. At index of mesor from 90 to 113 and at ultradiane rhythm the isolated strain is identified as C. albicans. Method doesn't require large material consumptions for equipment and nutrient media and allows carrying out identification of pathogens for 24 h and with high reliability.
 Method for accelerated assay of sensitivity of burckholderiae to chemopreparations / 2319746
Invention relates to an accelerated method for assay of sensitivity of burkcholderiae to chemopreparations. Accelerated assay method is based on using a solid nutrient medium with an indicator. Medium contains tryptone and glucose with indicator bromothymol blue, pH 6.0-7.6 (acid - yellow color, alkaline - blue color), the parent pH of medium is 6.8, and method involves preliminary preparing burkcholderiae to be analyzed before their inoculation. For carrying out the assay a medium is prepared of the following composition, g/l: tryptone, 2.0; NaCl, 5.0; K2HPO4, 0.3; bromothymol blue, 0.08; agar, 15.0, and distilled water, up to 1 l, pH 6.8. Sterile glucose solution (10 g/l) is added to medium before pouring plates at temperature 45°C and poured by a layer of thickness 4.0 ± 0.5 mm before 24 h for carrying out the assay. Analyzed culture of microorganisms is diluted to the concentration 108 cells/ml in 0.85% NaCl heated to 37°C and maintained this temperature up to inoculation moment. Then suspension of analyzed strain of burkcholderiae is applied on heated plates with medium in the amount 108 cells in volume of 0.2 ml of water. Then disks impregnated with antibiotics are places on agar surface. After 3-5 h of incubation (depending on the pathogen species) result is recorded: microorganisms retention zone growth around disks remain of green-like color but in zones of cultures growth without antibiotics medium turn yellow based on acidification of medium as result of glucose destruction by multiplying microorganisms. Method provides accelerating assay of sensitivity of burkcholderiae to chemopreparations, significant decrease for recording results on solid nutrient medium with indicator as compared with using the standard medium (3-5 h and 18-24 h, respectively).
Method for predicting tuberculous pleuritis / 2318209
The present innovation refers to bacteriological diagnostics of tuberculous pleuritis. It is necessary to apply fibrin deposit obtained from the surface of pleural cavity pouches to be inoculated onto nutritive medium for subsequent bacteriological survey. Inoculation should be fulfilled onto nutritive medium called "Novaya". The innovation provides the chance to diagnose tuberculous pleuritis at earlier stages of its development.
 Medium for assay of hemolytic activity of culture of genus listeria / 2318022
Invention proposes a medium consisting of two layers - backing agar and covering agar. Backing agar comprises agar "Difko" and physiological solution. Covering agar comprises agar "Difko", yeast extract, dithiothreitol rabbit fibrin-free blood, sodium chloride, and beef-extract broth or Hottinger broth as a nutrient base. Invention provides study the listeriolysin expression level, to carry out total screening and differentiation of Listeria strains population based on feature of their hemolytic activity. Invention can be used in bacteriological diagnosis of listeriosis in research aims for characterization of strains by their biological activity.
Method for differentiating human olfactory mucosa staphylococcus microflora / 2318021
Method involves isolation of staphylococcus pure cultures from olfactory mucosa microflora, their identification and capacity of pure culture for inactivating natural dipeptide carnosine is determined. Microorganism S. aureus is related to normal human olfactory mucosa microflora that is not able for activating carnosine (anti-carnosine activity - ACaA), and other species of staphylococci possessing ACaA in the level of expression of this feature 0.7 mcg/ml or less. Also, S. aureus showing the expression level of ACaA above zero is related also, and other species of staphylococci in the expression level of this feature above 0.7 mcg/ml. Invention provides the information content in assay of the presence and expression level of ACaA in staphylococci. Invention can be used in practice work of bacteriology laboratories for differentiation of human olfactory mucosa microflora for normal staphylococcus microflora and staphylococcus microflora able to induce development of local suppurative-inflammatory diseases.
Strain of bacterium yersinia pestis used as test-strain of central-caucasus high mountains plague location / 2317325
Invention reports the strain of bacterium Yersinia pestis C-781 obtained by direct inoculation if visceral organs of mountain suslik on solid Hottinger agar at pH 7.2 ± 0.2 unit. The strain is passed five times through body fleas Xenopsylla cheopis representing active carriers of plague and stored on Hottinger slant agar at pH 7.2 ± 0.2 unit in soldered tubes at temperature +4°C for one year and used then for infection of laboratory animals (white mice, guinea pigs). Found capacity for retention and recovery of virulence, stability of biological properties of the strain allow using the strain Yersinia pestis C-781 as test-strain for detecting strains of plague microbe in Central-Caucasus high mountains natural location of plague.
Nutrient medium for isolation and culturing mycobacteria from biological material / 2315812
Invention proposes nutrient medium that comprises as the nutrient base the following components: hen yolk egg aqueous solution, potassium hydrogen phosphate, magnesium sulfate, L-asparagine, glycerol, malachite green, citric acid, ferrous ammonium citrate, humivite, agar and distilled water. Invention provides increasing the growth rate of tuberculosis mycobacteria and to enhance purity in their indication from biological material. Invention can be used in bacteriological diagnosis of tuberculosis.
Method for diagnosis of sensitivity of mycobacterium tuberculosis to antituberculosis preparations / 2315113
Method involves sampling sputum in a patient, its preparing, seeding prepared sputum into tubes with Levenstein-Jensen medium, keeping tubes in thermostat, addition of Griss reagent and recording results by change of the nutrient medium color. Invention provides decreasing time for research of drug sensitivity and reducing the cost of a method for assay of sensitivity of Mycobacterium tuberculosis to antituberculosis preparations. Invention can be used for assay of sensitivity of Mycobacterium tuberculosis to antituberculosis preparations.
Brevibacillus laterosporus bacterium strain inhibiting and preventing development of microphytic algae of various taxonomic types / 2323968
Brevibacillus laterosporus "ВКПМ" В-9405 bacterium strain is separated by means of multistage selection from the natural Brevibacillus laterosporus "ВКПМ" В-8287 strain. The algicide activity is estimated by the strain lytic action on microalgae defining residual optical density (OD). OD is 10.1%. The strain algistatic activity is estimated by defining optical density. The optical density is 1.950.
 Preparation for soil cleaning from arsenic and protection of plants from diseases inhibited by plant pathogenic fungi and preudomonas aureofaciens arc v-2390 d bacterium strain for its making / 2323967
Preudomonas aureofaciens KR31 (pKS1) strain separated from the rhizosphere of alfalfa in Krasnodar Territory and deposited to the All-Russian collection ARC V-2390 D, is capable of inhibiting growth of a wide range of plant pathogenic fungi, stimulates plat growth, resistant to arsenic, dissolves phosphates. Preparation containing Preudomonas aureofaciens bacterium strain cells suspension may be used to clean the soil from arsenic and to protect the plants from the diseases, caused by plant pathogenic fungi.
Bioactive organomineral slow-release fertilizer / 2323918
Organomineral fertilizer, which contains rock phosphate (apatite), natural zeolite, oxidised brown coal in 1:0.15:2-1:0.2:5 ratio, is composted for 30-60 days, while exposed to phosphate-assimilating bacteria in amount of 105-106 cells/spores/ml, isolated from local soil using selection method. Content of labile phosphorus increases from 17.0 to 50.4 mg/kg of soil, and phosphatase activity in chestnut soil increases by 2.8-21.8 times.
Method for preparing halophilic bacterium biomass / 2323251
Method involves culturing halophilic microorganisms in the presence of encapsulated adsorbent and/or antioxidant up to the stationary growth phase in preparing the seeding material. Then in submerged culturing fresh medium is added by portions in the amount 1/3 of the final volume of cultural fluid followed by contacting cultural fluid with an encapsulated adsorbent and/or antioxidant, and additional feeding with dry or concentrated medium is carried out. The amount of dissolved oxygen is maintained at the level 5-10% of the equilibrium level. Invention provides increasing the level of accumulation of biomass, to enhance the specific content of bacteriorhodopsin in halophilic microorganisms biomass and the total yield of bacteriorhodopsin at the minimal content of carotinoids in biomass, and to reduce consumption of nutrient medium also.
Method for preparing halophilic bacterium biomass / 2323226
Method involves using an encapsulated adsorbent and/or antioxidant in preparing seeding material and submerged culturing. In preparing seeding material the culturing process is carried out in freshly prepared nutrient medium up to the stationary growth phase. Submerged culturing is carried out by simultaneous inoculation of bioreactor with seeding material and contacting the content of bioreactor with encapsulated adsorbent and/or antioxidant. The oxygen content in bioreactor is maintained at the level 5-10% of the equilibrium level. Invention provides increasing yield of halophilic microorganisms biomass up to 42 g/l and with the content of bacteriorhodopsin up to 1.6 g/l and in practically absent of carotinoids in biomass. Invention provides reducing time for preparing the unit mass of the end product and to decrease consumptions for production of bacteriorhodopsin.
Nutrient medium for culturing mycobacterium and nocardioformous actinomyces / 2322495
Invention proposes a nutrient medium comprising potassium hydrogen phosphate, magnesium sulfate, L-asparagine, glycerol, citric acid, ferrous ammonium citrate, agar, humivite and distilled water. Invention provides enhancing growth properties of the nutrient medium.
 Microorganism strain bacillus simplex as producer of site-specific endonuclease blsi / 2322494
Invention proposes the strain Bacillus simplex 23 isolated from soil and providing preparing site-specific endonuclease. This enzyme is able for recognizing and cleaving both chain in nucleotide sequence of DNA comprising at least one C5-methylcytosine base in the recognition site 5'-GCNGC-3' to form 3'-prominent ends. The novel strain can be used for isolation of the novel site-specific endonuclease that can be used for detection and cleavage of methylated sites in DNA.
Microorganism strain paracoccus denitrificans as producer of exopolysaccharide and exopolysaccharide / 2322493
Invention relates to a novel culture of microorganism producing high-molecular exopolysaccharide. Invention proposes the strain of microorganism Paracoccus denitrificans VKPM B-8617 that produces exopolysaccharide possessing cross-linking properties in aqueous and water-containing hydrocarbon systems. Exopolysaccharide is formed by residues of glucose, galactose, mannose and rhamnose in the ratio = 52:4:1, respectively, and comprises glucuronic and pyruvic acids, and acyl groups also and has molecular mass (0.5 x 106)-(2 x 107) Da. This exopolysaccharide is able to form pseudoplastic and thixotropic highly viscous solutions showing stable values of dynamic viscosity in the range of temperature from 20°C to 90°C and unstratifying emulsions. Proposed exopolysaccharide can be used in building, paper, textile, perfume-cosmetic, food, chemical, oil- and gas-extracting industry, agriculture, and in pharmaceutics and medicine.
 Glacial ice bacterium microorganism strain as producer of site-specific endonuclease glu i / 2322492
Strain of microorganism Glacial ice bacterium is isolated from soil and can be used for a preparing site-specific endonuclease that recognizes and cleaves both chain of methylated nucleotide sequence in DNA: 5'-G(m5C)↓NG(m5C)-3'. Use of the invention provides preparing the novel site-specific endonuclease showing specificity to a methylated sequence in DNA that can be used in DNA analysis.
Method of biologically processing bird dung / 2322427
Method envisages mixing bird dung with moisture-absorbing material followed by aerobic fermentation of the mixture in presence of microorganisms. Process is carried out at stirring until temperature of fermentation mixture spontaneously drops to 25-30°C, said microorganisms being consortium of strains: Bacillus subtilis B-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-9-7, Candida tropicalis Y-1520, Candida utilis Y-2441. Preferable implementation of the method is when above-listed strains are used in equal proportions and in amount of 1•108-1•109 cells in 1 mL per 1 ton bird dung.
 Strain of lactobacilli lactobacillus paracasei cncm i-2116 (ncc 2461) eliciting ability to prevent intestine colonization with pathogenic microorganisms causing diarrhea, supernatant of its culture and oral agent for prophylaxis and/or treatment of diarrhea-associated disorders / 2243779
Invention relates to the strain Lactobacillus paracasei CNCM I-2116 used for diarrhea prophylaxis causing by pathogenic microorganisms. Supernatant of this strain culture elicits ability to prevent colonization of intestine with pathogenic microorganisms causing diarrhea also and this strain is designated for preparing agent used for prophylaxis and/or treatment of disorders associated with diarrhea. Agent for oral administration represents therapeutically effective dose of the strain L. paracasei CNCM I-2116 or supernatant of its culture and acceptable foodstuff. Invention provides the enhanced viability of the strain in its applying and effectiveness in prophylaxis of adhesion to intestine cells and invasion to intestine cells of pathogenic microorganisms causing diarrhea.
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FIELD: medicine. SUBSTANCE: nutrient medium contains the pancreatic casein hydrolysate, enzymatic peptone, activator of hemophilic microorganisms' growth, bread yeast, lactose, bromocresol purple, glucose, L-cysteine, sodium sulfite, sodium citrate, sodium azide, crystal violet, agar-agar, distilled water. EFFECT: growth activity of the nutrient medium is increased while the cost goes down. 1 tbl, 3 ex
The invention relates to medicine, namely to clinical Microbiology, and can be used for the diagnosis of streptococcal infections. Setting accurate etiological diagnosis of streptococcal pharyngeal and skin infections in all cases, except for scarlet fever requires microbiological research involving the isolation and species identification of streptococci. To date, in clinical practice there are no commercial nutrient medium for isolation of streptococci. The only commercial environment - the environment to highlight hemoculture and cultivation of streptococci ("Allergen", Stavropol) is not produced now. In clinical practice the country to highlight streptococci are environment laboratory preparation: nutrient agar or trypticase-soy agar with the addition of 3-5% defibrinating blood [3], nutrient broth with added bovine serum (10%) or glucose (0.2%) of [10], mastopathy agar with 5-7% defibrinating blood rabbit or sheep [7]. To suppress the growth of foreign microflora in the cooking process environment in the laboratory add antibiotics [4, 5]. As a rule, the quality and standardization of these environments, with the addition of biological liquids that do not always meet requirements require the requirements. Abroad developed a nutrient medium for isolation of streptococci, which as a selective agent used is sodium azide [8, 13, 14]. But not every practical laboratory able to buy them due to high prices, the lack of commercial availability limits their use to decrypt streptococcosis. In their focus and set of features closest to the claimed invention is Streptosel agar containing as sources of nitrogen nutrition pancreatic hydrolysate of casein, papirovy hydrolyzed soy flour source of carbohydrate nutrition - glucose, as inhibitors of gram-positive and gram-negative microflora - sodium azide, sodium citrate, sodium sulfite, crystal violet, and L-cystine, sodium chloride, agar [13]. The disadvantages of this environment, adopted for the prototype are the following: Streptosel agar is a foreign environment, roads and beyond the reach of many laboratories in the country; secondly, guidelines for the microbiological diagnosis of streptococcal infections is selected (7) the need for differentiation and hemolytic viridans streptococci with enterococci [10]. The obstacle to achieving the above technical result in this environment, which we use as a prototype, is the fact that it has no differentiating properties and suggests further identification of selected cultures of streptococci by additional tests. Last lengthens the time of diagnosis. The task of the invention is the improvement of growth and giving differentiating properties of the environment. This technical result in the implementation of the invention is achieved by the fact that as growth stimulants streptococcal she additionally contains peptone is an enzymatic, growth factors Haemophilus organisms, extract bread yeast [9, 11, 12]. To give the differentiating properties of the drug have been added to the indicator system, consisting of lactose and of dye bromocresol purple. The environment also contains pancreatic hydrolysate of casein (tripton) [9], glucose, sodium citrate, sodium sulfite, L-cystine, sodium azide, crystal violet agar in the following ratio of components, g/l distilled water:
The proposed recipe is different from the recipe prototype on the following principles. 1. Put the indicator system comprising lactose and the indicator bromocresol purple. Methodical recommendations offer an advanced scheme for the differentiation and hemolytic viridans streptococci with enterococci, takes 3 or more days and the need for multiple culture media and test systems for subsequent species identification of streptococci. The proposed environment to highlight streptococci (Streptococcus-azide agar) introduced in the composition of the carbohydrate and the dye provides a clear differentiating properties and allows to identify at least 3 species of streptococci. On crimson agar plate constructed environment observed within 18-36 hours of incubation, the growth of gray shiny translucent colonies pyogenic Streptococcus. Colony viridans Streptococcus - blue, wet Shine; colonies of fecal Streptococcus - KRU is nye, dense, gray-yellow color. Thus the environment to highlight streptococcal reduces the time allocation of net culture and the definition of its species, which speeds up the diagnosis. 2. In the drug-balanced nutrient base medium consisting of a combination of pancreatic hydrolysate of casein, peptone enzymatic and enriched with growth factors (growth factors Haemophilus organisms and extract bread yeast). In contrast to "classical" culture media for isolation of streptococci requiring blood or serum [1], nutrient basic Foundation promotes optimal growth of Streptococcus increasing the size of the colonies, improving the morphology and, hence, differentiating properties, without the addition of biological fluids. All part of the recipe - gastromony ingredients of domestic production. The medium was prepared as follows. Example 1. In 1 l of distilled water make 29,0 g pancreatic hydrolysate of casein, 19,0 g peptone is an enzymatic, 2.4 g of glucose, 9.0 g of lactose, 4.9 g of the extract of bread yeast, 9.0 g of a growth stimulant Haemophilus organisms, 0.9 g of sodium citrate, 0,19 g L-cystine, 0,19 g of sodium sulfite, to 0.19 g of sodium azide, 0,0019 g crystal violet, and 11.0 g of agar microbiological, 0,15 bromcresol purple. Thoroughly mixed, the resulting suspension is brought to a boil and boil until complete melt the agar. pH 7,4±0,2. The medium is cooled to a temperature of 45-50°and poured into sterile Petri dishes with a layer of 4.0-5.0 mm, left to freeze. Before sowing Cup with medium dried in a thermostat for 40-60 minutes at a temperature of 37°C. the Selection of clinical samples, prepare them for research and planting on Wednesday carried out in accordance with the recommendations contained in the Microbiological diagnosis of streptococcal infections" (M, 1996). Incubation is carried out in "candle" the vessel in an atmosphere containing carbon dioxide. The results take into account in 24-48 hours of incubation. Example 2. In 1 l of distilled water make 30.0 g of pancreatic hydrolysate of casein, 20,0 g peptone is an enzymatic, 2.5 g glucose, 10.0 g lactose 5.0 g of the extract of bread yeast, 10.0 g of a growth stimulant Haemophilus organisms, 0.2 g of L-cystine, 1.0 g of sodium citrate, 0.2 g of sodium sulfite, 0.2 g of sodium azide, 0.002 g of crystal violet, 0.16 g of bromocresol purple, 11,75 g of agar microbiological. Further, according to the example 1. Example 3. In 1 l of distilled water make 31.0 g of pancreatic hydrolysate of casein, 21,0 g peptone is an enzymatic, 2.6 g of glucose, 10,1 g of lactose, and 5.2 g of extract bread yeast, 10.1 growth of Haemophilus microor is Anisimov, 0.21 g of L-cystine, 1.1 g of sodium citrate, 0.21 g of sodium sulfite, 0.21 g of sodium azide, 0.0022 g of crystal violet, 0.17 g of bromocresol purple, 12.5 g agar microbiological. Further, according to the example 1. Biological characteristics of the environment to highlight streptococci (Streptococcus-azide agar) and the prototype presented in the table. The table shows that the proposed environment has the advantage compared with the prototype environment. The proposed environment of the colony streptococci larger, which is important for visual display indication of streptococcal colonies having on our environment, different coloring. In a prototype environment colonies of streptococci smaller, white, differentiation is absent.
BIBLIOGRAPHIC DATA 1. Birger MO Handbook of microbiological and virological methods. M.: Medicine, 1982, s.255-256. 2. BRIC NI Diseases caused by Streptococcus group a at the beginning of the 21st century: problems and prospects for prevention. Bulletin of the AMS, 2001, No. 2. 3. BRIC NI, Edina A.S., Rapes L.A. Laboratory diagnosis of streptococcal infections. A manual for physicians and researchers. M: Grissom, 2000, p.26. 4. Zatsiorsky S.L. Evaluation of different nutrient media in the allocation streptococcal group of clinical materials. Laboratory work, 1989, No. 3, p.15-17. 5. Igonina Y.A., Shevchuk, MS, Bochkov I.A., Osman S. Kaliev Selection of culture media for laboratory diagnosis of streptococcal group Century Journal. microbiol., Epidemiol. and infects. bol., 1983, No. 9, p.21-24. 6. Guidelines for the control of nutrient media on biological indicators. M., 1980. 7. Guidance on the microbiological diagnosis of infectious diseases. Edited by Matveev K.I. and M.I. Sokolov, M., 1964, s. 8. Guidance on the application of the company's products Himedia laboratory practice. 2003, p.75.9. Guide to Microbiology, clinical picture and epidemiology of infectious diseases. Edited by Zhukov-Verezhnikova N.N., V.1, M., 1962, s-348, 354-355. 10. Streptococcal infection. II. Microbiological diagnosis of streptococcal infections. Methodical recommendations. Publishing house "Grant", 1999, p.15. 11. Telichevesky L.Y. Protein hydrolysates. M, 2000, s-247. 12. Physico-chemical methods of control of the ingredients of culture media and biological products. Edited by Kolesov YEAR M., 1970, p.63-65. 13. Manual of BBL. Products and laboratory procedures. Sixth Edition. Cat. No. 5200, 1988, p.254. 14. Microbiology Merck Manual. Darmstadt, 1990, p.192. Nutrient medium for the selective extraction streptococci containing a nutrient basis, glucose, L-cystine, sodium sulfite, sodium citrate, sodium azide, crystal violet, microbiological agar, distilled water, characterized in that it further as a source of nitrogen nutrition it contains peptone is an enzymatic, as growth stimulants boost the growth of microorganisms Haemophilus and extract bread yeast, and as an indicator of system - lactose and bromocresol purple in the following ratio of components, g/l distilled water:
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