Brevibacillus laterosporus bacterium strain inhibiting and preventing development of microphytic algae of various taxonomic types |
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IPC classes for russian patent Brevibacillus laterosporus bacterium strain inhibiting and preventing development of microphytic algae of various taxonomic types (RU 2323968):
 Preparation for soil cleaning from arsenic and protection of plants from diseases inhibited by plant pathogenic fungi and preudomonas aureofaciens arc v-2390 d bacterium strain for its making / 2323967
Preudomonas aureofaciens KR31 (pKS1) strain separated from the rhizosphere of alfalfa in Krasnodar Territory and deposited to the All-Russian collection ARC V-2390 D, is capable of inhibiting growth of a wide range of plant pathogenic fungi, stimulates plat growth, resistant to arsenic, dissolves phosphates. Preparation containing Preudomonas aureofaciens bacterium strain cells suspension may be used to clean the soil from arsenic and to protect the plants from the diseases, caused by plant pathogenic fungi.
Bioactive organomineral slow-release fertilizer / 2323918
Organomineral fertilizer, which contains rock phosphate (apatite), natural zeolite, oxidised brown coal in 1:0.15:2-1:0.2:5 ratio, is composted for 30-60 days, while exposed to phosphate-assimilating bacteria in amount of 105-106 cells/spores/ml, isolated from local soil using selection method. Content of labile phosphorus increases from 17.0 to 50.4 mg/kg of soil, and phosphatase activity in chestnut soil increases by 2.8-21.8 times.
Method for preparing halophilic bacterium biomass / 2323251
Method involves culturing halophilic microorganisms in the presence of encapsulated adsorbent and/or antioxidant up to the stationary growth phase in preparing the seeding material. Then in submerged culturing fresh medium is added by portions in the amount 1/3 of the final volume of cultural fluid followed by contacting cultural fluid with an encapsulated adsorbent and/or antioxidant, and additional feeding with dry or concentrated medium is carried out. The amount of dissolved oxygen is maintained at the level 5-10% of the equilibrium level. Invention provides increasing the level of accumulation of biomass, to enhance the specific content of bacteriorhodopsin in halophilic microorganisms biomass and the total yield of bacteriorhodopsin at the minimal content of carotinoids in biomass, and to reduce consumption of nutrient medium also.
Method for preparing halophilic bacterium biomass / 2323226
Method involves using an encapsulated adsorbent and/or antioxidant in preparing seeding material and submerged culturing. In preparing seeding material the culturing process is carried out in freshly prepared nutrient medium up to the stationary growth phase. Submerged culturing is carried out by simultaneous inoculation of bioreactor with seeding material and contacting the content of bioreactor with encapsulated adsorbent and/or antioxidant. The oxygen content in bioreactor is maintained at the level 5-10% of the equilibrium level. Invention provides increasing yield of halophilic microorganisms biomass up to 42 g/l and with the content of bacteriorhodopsin up to 1.6 g/l and in practically absent of carotinoids in biomass. Invention provides reducing time for preparing the unit mass of the end product and to decrease consumptions for production of bacteriorhodopsin.
Nutrient medium for culturing mycobacterium and nocardioformous actinomyces / 2322495
Invention proposes a nutrient medium comprising potassium hydrogen phosphate, magnesium sulfate, L-asparagine, glycerol, citric acid, ferrous ammonium citrate, agar, humivite and distilled water. Invention provides enhancing growth properties of the nutrient medium.
 Microorganism strain bacillus simplex as producer of site-specific endonuclease blsi / 2322494
Invention proposes the strain Bacillus simplex 23 isolated from soil and providing preparing site-specific endonuclease. This enzyme is able for recognizing and cleaving both chain in nucleotide sequence of DNA comprising at least one C5-methylcytosine base in the recognition site 5'-GCNGC-3' to form 3'-prominent ends. The novel strain can be used for isolation of the novel site-specific endonuclease that can be used for detection and cleavage of methylated sites in DNA.
Microorganism strain paracoccus denitrificans as producer of exopolysaccharide and exopolysaccharide / 2322493
Invention relates to a novel culture of microorganism producing high-molecular exopolysaccharide. Invention proposes the strain of microorganism Paracoccus denitrificans VKPM B-8617 that produces exopolysaccharide possessing cross-linking properties in aqueous and water-containing hydrocarbon systems. Exopolysaccharide is formed by residues of glucose, galactose, mannose and rhamnose in the ratio = 52:4:1, respectively, and comprises glucuronic and pyruvic acids, and acyl groups also and has molecular mass (0.5 x 106)-(2 x 107) Da. This exopolysaccharide is able to form pseudoplastic and thixotropic highly viscous solutions showing stable values of dynamic viscosity in the range of temperature from 20°C to 90°C and unstratifying emulsions. Proposed exopolysaccharide can be used in building, paper, textile, perfume-cosmetic, food, chemical, oil- and gas-extracting industry, agriculture, and in pharmaceutics and medicine.
 Glacial ice bacterium microorganism strain as producer of site-specific endonuclease glu i / 2322492
Strain of microorganism Glacial ice bacterium is isolated from soil and can be used for a preparing site-specific endonuclease that recognizes and cleaves both chain of methylated nucleotide sequence in DNA: 5'-G(m5C)↓NG(m5C)-3'. Use of the invention provides preparing the novel site-specific endonuclease showing specificity to a methylated sequence in DNA that can be used in DNA analysis.
Method of biologically processing bird dung / 2322427
Method envisages mixing bird dung with moisture-absorbing material followed by aerobic fermentation of the mixture in presence of microorganisms. Process is carried out at stirring until temperature of fermentation mixture spontaneously drops to 25-30°C, said microorganisms being consortium of strains: Bacillus subtilis B-168, Bacillus mycoides B-691, Bacillus mycoides B-46, Streptococcus thermophilus B-9-7, Candida tropicalis Y-1520, Candida utilis Y-2441. Preferable implementation of the method is when above-listed strains are used in equal proportions and in amount of 1•108-1•109 cells in 1 mL per 1 ton bird dung.
Biopreparation for enhancing productivity of agricultural crops and improving quality of production / 2322061
Invention relates to biopreparations of effective microorganisms used in the plant growing. Proposed preparation consists of a mixture of suspension of the following strains deposited in VKPM: Agrobacterium tumefacients B-4116, Agrobacterium radiobacter B-956, Azotobacter chroococcum B-2375, Bacillus thuringiensis B-2918, Bacillus subtilis B-6554, Bacillus subtilis B-4419, Bacillus megaterium B-4440, Bacillus megaterium B-200, Bradyrhizobium japonicum B-1978, Ervinia ananas B-5292, Lactobacillus casei B-3961, Pseudomonas fluorescens B-1138, and Rhodopseudomonas palustris B-1629. Invention provides restoring soil and to improve its structure, increase germination of seeds, fortify immune system of plants, enhance resistance against diseases and pests that increases the yield significantly and high quality of the prepared foodstuffs. Proposed biopreparations can be used for autumn, spring and summer treatment of soil, roots and leaf feeding, and for presowing treatment of seeds.
 Strain of lactobacilli lactobacillus paracasei cncm i-2116 (ncc 2461) eliciting ability to prevent intestine colonization with pathogenic microorganisms causing diarrhea, supernatant of its culture and oral agent for prophylaxis and/or treatment of diarrhea-associated disorders / 2243779
Invention relates to the strain Lactobacillus paracasei CNCM I-2116 used for diarrhea prophylaxis causing by pathogenic microorganisms. Supernatant of this strain culture elicits ability to prevent colonization of intestine with pathogenic microorganisms causing diarrhea also and this strain is designated for preparing agent used for prophylaxis and/or treatment of disorders associated with diarrhea. Agent for oral administration represents therapeutically effective dose of the strain L. paracasei CNCM I-2116 or supernatant of its culture and acceptable foodstuff. Invention provides the enhanced viability of the strain in its applying and effectiveness in prophylaxis of adhesion to intestine cells and invasion to intestine cells of pathogenic microorganisms causing diarrhea.
Strain of microorganism lactobacillus buchneri 66 for ensilage of maize green mass and preserving flattened grain / 2243999
The strain Lactobacillus buchneri 600 is isolated from barley grains at milkwax stage of ripeness. The strain Lactobacillus buchneri 600 is registered in 05. 08. 2002 year in the All-Russian State collection of microorganism strains used in veterinary science and animal husbandry at number Lactobacillus buchneri VGNKI 02.08.04-DEP and deposited in the collection 000 "Biotrof". Invention provides the more effective multiplication of the strain in maize ensilaging green mass and preserving flattened grains with enhanced formation of lactic acid, inhibition of putrid microflora that allows preparing fodder from vegetable raw with enhanced quality. Invention can be used in fodder production for ensilage maize green mass and preserving flattened grains.
Strain lactobacillus acidophilus as producer of fodder protein / 2244000
Invention relates to a new isolated strain of Lactobacillus acidophilus 1660/08 as a producer of the protein fodder. The strain Lactobacillus acidophilus 1660/08 is obtained by the selection method and selected by its ability to form significant amount of crude protein and to accumulate the biomass. The strain is deposited in the VGNKI collection at number VGNKI-03.04.10.-DEP. Invention provides eliminating the environment pollution in producing the protein fodder, to enhance the specific protein yield, to reduce energy consumptions in preparing protein fodder, to simplify and to accelerate the process in its preparing, to simplify equipment fitting out and to utilize waste in manufactures using natural raw.
Strain lactobacillus plantarum as producer of fodder protein / 2244001
The strain Lactobacillus plantarum 578/25 is obtained by method of step-by-step selection and selected by its ability to produce significant amount of crude protein and to accumulate the biomass. The strain is deposited in the VGNKI collection at number VGNKI-03.04.09.-DEP. Invention provides eliminating the pollution of environment in producing the protein fodder, to elevate the protein specific yield, to reduce energy consumptions in preparing protein fodder, to simplify and to accelerate the process of its preparing, to simplify apparatus equipment, to utilize waste in manufacturing using the natural raw.
 Strain of microorganism lactobacillus lactis for preparing curd from milk / 2244002
The strain of microorganism Lactobacillus lactis VKPM B-8354 is prepared without using mutagens and genetic methods and shows resistance against broad spectrum of lactophages. The strain ferments effectively milk from different trading sorts, with broad range of fatness and different methods of thermal treatment. Individual specific properties of the strain allows its applying as a monostrain ferment. Curd obtained with applying the strain L. lactis VKPM B-8354 shows good organoleptic qualities, nonacid taste and homogenous consistence. The strain is suitable especially for plants with small volume of manufacture but with varied assortment.
Method for isolation and selection of microorganisms as producers of cyclodextrin glucanotrasnferase, strain of microorganism bacillus circulans b-65 ncaim (p) 001277 (b-65) as producer of extracellular cyclodextrin transferase, cyclodextrin glucanotransferase obtained from its and its applying for preparing cyclodextrin / 2244742
The strain Bacillus circulans B-65 a producer of cyclodextrin glucanotransferase is isolated and selected form the soil sample by culturing in nutrient medium with amylolytic activity 12.17 U/ml and cyclodextrinogenic activity up to 0.530 U/ml. Cyclodextrin glucanotransferase isolated from B. circulans B-65 shows the high degree for conversion of starch to cyclodextrins and this enzyme is specific for formation of β-cyclodextrin. Invention can be used in food industry for preparing cyclodextrins and cyclodextrin glucanotransferase used in different branches of industry.
Bacterial preparation, method for its producing, nutrient medium for culturing cells escherichia coli vkm cr-322d and method for prophylaxis and treatment of gastroenteric disease in agricultural and domestic animal and poultry / 2244743
For preparing a preparation cells of microorganism Escherichia coli VKM CR-322D is cultured in nutrient medium containing Hottinger's broth, glucose, yeast extract, manganese sulfate, potassium hydrophosphate, sodium chloride and tap water in the content of amine nitrogen 125-155 mg%. Glucose is added by batch portions in the process of culturing cells that is carried out at temperature 30-31oC at stirring and aeration for 10-12 h. Prepared cultural fluid containing 3 x 109 bacterial cells/ml is mixed with protective sucrose-gelatin medium and subjected for lyophilic drying. Dried mass is stored under nitrogen that enhances safety of viable cells in the preparation. Applying the preparation for prophylaxis and treatment of agricultural and domestic animals and poultries with gastroenteric diseases provides its effectiveness.
Method for preparing liquid lactobacterin / 2244744
Method for preparing liquid lactobacterin involves regeneration, culturing passages of lyophilized culture and culturing ferment of lactobacilli in liquid lyophilized nutrient medium containing dry defatted milk enzymatic hydrolyzate with the content of amine nitrogen 1 485 mg%, 30.0 ± 3.0 g/l; yeast concentrated autolyzate, 110.0 ± 10 g/l; food agar, 0.8 g/l, and distilled water, up to 1 l. Culturing ferment is carried out up to accumulation of biomass of lactobacilli 109-1010 CFU/ml. Then 10-30% of supernatant liquid is removed from the ready product and replaced it with equal volume of fresh nutrient medium. Invention provides simplifying technology in preparing liquid lactobacterin and to elevate the storage period of viable lactobacilli. Invention can be used in producing probiotic preparations.
Strain bifidobacterium longum 379-in used for preparing bacterial preparations, biologically active supplements for food, ferments, fermented-dairy and nonfermented dairy foodstuffs, hygienic and cosmetic agents / 2244745
The strain Bifidobacterium longum 379-IN is obtained by selection without using methods of genetic modification of the strain Bifidobacterium longum B379M and distinct by ability to utilize insulin. The strain is deposited in GKNM GU "MNIIEM named for G. N. Gabrichevskiy Russia Ministry of Public Health" at № 172. The strain shows high technological effectiveness, accumulates biomass with substrates of vegetable origin and artificial nutrient media for short periods with concentration of bifidobacteria, it elicits acid-forming and antagonistic properties with respect to pathogenic and putrid microflora. This allows its using in manufacturing bacterial preparations, biologically active supplements for food, fermented-dairy and nonfermented-dairy foodstuffs, ferments, hygienic and cosmetic agents providing probiotic effect and normalization of microbiocenosis in human body, among them in gastroenteric and urogenital tracts, cutaneous and mucosa integuments. Invention can be used in manufacturing bacterial preparations, biologically active supplements for food, fermented-dairy and nonfermented-dairy foodstuffs, hygienic and cosmetic agents.
Nutrient medium for culturing plague microorganism vaccine strain / 2245362
Invention relates, in particular, to preparing nutrient media used for culturing the plague microorganism vaccine strain and can be used in medicinal microbiology. The nutrient medium for culturing the plague microorganism vaccine strain comprises additionally as a stimulating additive sodium sulfite and as a nutrient base - soybean fruits enzymatic hydrolyzate in the following ratio of components, g/l: microbiological agar, 11.0-13.0; soybean fruits enzymatic hydrolyzate, 250.0-350.0; sodium chloride, 4.5-5.5; sodium hydrogen phosphate, 3.5-4.5; sodium sulfite, 0.0003-0.0005; distilled water, the balance. Invention provides enhancing the growth property of nutrient medium.
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FIELD: agriculture. SUBSTANCE: Brevibacillus laterosporus "ВКПМ" В-9405 bacterium strain is separated by means of multistage selection from the natural Brevibacillus laterosporus "ВКПМ" В-8287 strain. The algicide activity is estimated by the strain lytic action on microalgae defining residual optical density (OD). OD is 10.1%. The strain algistatic activity is estimated by defining optical density. The optical density is 1.950. EFFECT: strain is capable of algicide and algistatic activity in relation to microphytic algae. 5 tbl, 5 ex
The invention relates to biotechnology, in particular to the use of biological control agents with microscopic algae. Increased anthropogenic pressure on water sources in the revenue of industrial, domestic and agricultural wastewater contaminates water bodies, as expressed in the "blooming" water, due to the development of different types of microscopic algae, primarily blue-green algae (cyanobacteria). Mass development of algae leads to the formation of three-dimensional structures, covering the water surface or "mats". The rapid growth of microalgae is manifested in the so-called "algal bloom in the development and, in the future, accompanied by the death of their excess biomass, production of toxins, impaired oxygen regime, the sensory manifestations of decay. Algal blooms result in changes in water quality, undesirable for residential, recreational, fisheries, and energy use. "Blooming" water is often observed in reservoirs, which supply water to the population and industry and represents a significant threat to the health of humans and animals. The ability of microalgae to rapid reproduction is associated primarily with their resistance to extreme temperatures and concentrationsa, low light, low transparency, a small amount of oxygen. Microalgae can grow in marine and fresh water and harm any water sources, both natural, technological and manmade. The algae live in water channels, pipes, swimming pools, aquariums. "Blooming" water can cause disruption to businesses that use this water. Serious economic damage causes "blooming" water power plants, nuclear reactors, etc. When death and decomposition of microalgae form decomposition products and toxins harmful to animals and humans [1-2]. Antidotes to the toxins of microalgae does not exist. In addition, microalgae cause a number of allergic diseases. Therefore, at high level "blooming" water is an undesirable use of water bodies for recreational purposes, i.e. you need to a minimum to limit the time spent by humans and domestic animals in such water. Using light energy, atmospheric nitrogen and the minimum content of mineral substances, microalgae create organic mass for growth of heterotrophic organisms, resulting in increased water pollution, and mass reproduction of the insect larvae. Water "blooming" water create favorable conditions for the breeding of larvae of the mosquito vector in which spadicea number of serious human diseases, including malaria. Thus, the algal bloom causes a number of heading social and economic consequences, which determines the necessity of the fight against blue-green bacteria and products of their metabolism. The urgency of the fight against algae and last years is increasing due to environmental pollution and the General warming of the Earth's climate, leading in some cases to rapid development of algae in different ecological niches, particularly in reservoirs. Currently, many countries are faced with the need to prevent development (legislationi effect) and/or suppression already growing algae (algaecidal effect). Used chemical and physical methods of control over the development of algae. Despite the effectiveness of chemical algaecides in the laboratory, mass, their use in vivo is limited by Toxicological and hygienic requirements. In addition, chemicals are not selective action and exert a number of biological effects (flying, mutagenic, teratogenic, and so on) on plant and animal organisms. The use of chemical algaecides for treatment of water bodies is limited by health and safety standards in connection with the danger to humans and animals and also adversely skazyvaetsa is in the other life aquatic (aquatic organisms). Used physical and chemical methods "struggle" with microalgae - the descent of water from reservoirs and subsequent mechanical removal of biomass aeration huge bodies of water, the use of chemical algaecide drugs and substances-coagulants, the use of ultraviolet irradiation, ultrasound is ineffective and is associated with high financial costs [3-4]. Physical control methods of microalgae is aimed at creating conditions, or preventing the development of algae or destroy already formed three-dimensional structure of the community of algae, the so - called "mats". Ultrasonic treatment of "blooming" water, although it is quite effective algaecidal action, leads to undesirable consequences. Processing "blooming" water ultrasound reduces pH, total nitrogen and phosphorus in the water, raise the water temperature. As an alternative to chemical and physical controls development of algae can be considered biological preparations based on the use of bacteria, providing antagonistic (algaecide) effect on algae. Currently, we have identified a number of microorganisms having algicidal effect. It can be expected that bacteria-antagonists will be a good source for creating modern algaecides. the biological method of controlling the numbers of microalgae can be used by microorganisms and their metabolites, providing an antagonistic effect on cyanobacteria, viruses, compounds of plant origin [5-6]. As the closest analogue of the claimed object is considered a strain of Sphingomonas sp.M-17, which causes lysis of cyanobacteria and different slow algicidal activity [7]. The task of the claimed inventions to obtain a bacterial strain having algicidal activity against a wide spectrum of microscopic algae. The problem is solved by obtaining the strain of bacteria Brevibacillus laterosporus, overwhelming and prevent the development of microscopic algae, belonging to different taxonomic types, as well as destroying educated microscopic algae three-dimensional structure, covering the water surface ("mats"). The inventive strain selected in a multistage selection from natural strain of Brevibacillus laterosporus VKPM B-8287 and deposited in Russian national collection of industrial microorganisms under the number VKPM B-9405. It differs from similar Sphingomonas sp.M-17 broad spectrum algaecide steps, faster canalithiasis effect lysis of cyanobacteria comes in 2 and 36 hours, respectively). The inventive strain of Brevibacillus laterosporus VKPM B-9405 has the following characteristics : 1. Cultural morphological traits Gram-negative motile Bacillus, p is retrai, size of 0.5-0.7×2.5 to 5.0 µm, chains do not form. Well sporulated on liquid and solid nutrient media. As a solid nutrient medium used agar medium NBY, LB and DPS. The composition of the agar medium NBY, wt.%: Nutrient broth 0,8 Yeast extract 0,3 Agar 2,0 Water The Rest. The composition of the agar medium (LB), wt.%: Bacto-triptan 1,0 Yeast extract 0,5 NaCl 1,0 Agar 2,0 Water The Rest. The composition of the agar medium DPS, wt.%: Yeast kaprinovye 3,0 Corn flour 1,5 Agar 2,0 Water The Rest. Under cultivation on agar medium NBY, LB and DPS through 48 hours at a temperature of 28-30°With the strain forms a rounded colonies with a diameter of 3-4 mm beige color with a smooth surface and a rough edge. After 72-96 hours of growth on nutrient medium NBY strain produces spores. In the process of sporulation is formed enomena structure adjacent to the dispute. Free spores are elliptical in shape. The size of the spores is 0.9-1.2×1,5-1,7 mm. 2. Physiological-biochemical properties The strain produces catalase. Hydrolyzes casein and gelatine, not hydrolyzes starch. Urea does not break. Not sprayway sucrose, arabinose, xylose, lactose. Sprayway glucose, maltose, mannitol, fructose. Glucose sprayway without gas. Does not form acetone. Forms lecithinase, twin ect the time. Grows in the presence of lysozyme. The range of possible cultivation of a strain of Brevibacillus laterosporus VKPM B-9405: pH 6.9-7.2 and a temperature of 28-31°C. the Optimum temperature 30°C and pH 7.0. 3. Maintaining the strain VKPM B-9405 spend on the shoals with agar medium NBY which subcultured every two weeks 4. Storing strain exercise after lyophilization. Vegetative cell culture grown on agar medium NBY. Cells wash away the protective medium containing sterilized skim milk. Ampoules with suspension cells incubated 15 min at -70°and then quickly transferred into a drying chamber connected to a vacuum system lyophilization (model 75150 firm "Labkonko"). Drying time was 4 hours. Lyophilized samples stored at a temperature of +4°C. 5. Cultivation of a strain of Brevibacillus laterosporus VKPM B - 9405 The inventive strain cultivated under standard conditions in katalozhnyh Erlenmeyer flasks in liquid nutrient medium NBY [(wt.%): nutrient broth (Nutrient broth "Difco")-8; yeast extract (Yeast extract Difco") 0,3] within 96 hours at 28-30°With shaking at 250 rpm/min See steady growth culture throughout the volume. By light microscopy and by plating on nutrient agar assess the presence of bacterial cells and spores and absence of extraneous microflora. To 96 hours of cultivation on logout mainly 90-95% of spores and 5-10% of the vegetative cells. Microbiological method to estimate the titer of colonies forming units (CFU) of strain VKPM B-9405, which amounts to 2.5 to 3.0 x 109/ml. 6. Algaecide and legistations properties of the strain were characterized using nitrogen-fixing filamentous species of microscopic algae cyanobacteria: Anabaena strain sp.5781 obtained from the Microbiology Department of the Leningrad state University; strain Nostoc sp.ATCC29411 obtained from the collection of IMET, Jena, Germany; two strains of nitrogen fixing cyanobacteria Microcystis aeruginosa 562 and 905 obtained from the Institute of Hydrobiology of the Chinese Academy of Sciences (Hubei province, guuchan); strains of green algae (Cosmarium) and marine algae (Amphidinium, Prorocentrum, Thalassiosira) received from the Department of Biophysics, Moscow state University. The inventive strain has a broad spectrum algaecide activity. Suppresses the development of the following microscopic algae (table 1).
7. Cultivation of microalgae used to identify algaecide and legislations properties of the strain. The algae Anabaena, Nostoc, Microcystis and Cosmarium were cultured at room temperature and the lighting mode day / night using fluorescent fluorescent white light LIGHT the FOREHEAD-30, providing illumination 1500-2000 lx, without aeration, glass flat-bottomed flasks with a capacity of 250 ml containing 50-100 ml of modified medium BG-11 (8), consisting of mixtures a and b of the following composition (wt.%):
After autoclaving and cooling medium pH is 7.1. Seaweed Amphidinium, Thalassiosira, Prorocentrum were grown in seawater under the same conditions of light and temperature. The invention is illustrated by the following examples Example 1. Assessment algaecidal activity of the strain VKPM B-9405. Algaecidal activity of the inventive strain was assessed by its lytic action on microalgae, registered a drop in optical density when mixed cultivation of the proposed strain with one of the cells of algae 96 hours joint incubation. To quantify algaecidal effect to the liquid culture of cyanobacteria (Nostoc, Anabaena, Microcystis) and green algae (Cosmarium) were added to 96-hour culture of the bacilli in the ratio of 5:1 and determined the residual optical density (ODAbout), as (soT/OPH)×100, where ODH- the initial OP, OPTOP after incubation for T hours, respectively. Measured optical density at a wavelength of 590 nm) sm is si in zero time and after incubation. Making the culture fluid of strain VKPM B-9405 caused the drop in optical density mixes it with the cells of microalgae as Nostoc and Anabaena 10 times, and with the cells of algae like Microcystis, and Cosmarium only twice (table 2). Lysis of cells of algae is also confirmed by optical microscopy.
It should be noted a significant turbidity mixtures strain VKPM B-9405 with cells of algae Microcystis and Cosmarium. To completion of the process of their co-culture with the claimed strain see the discoloration of these compounds, initially with a distinct color. Cyanobacteria Anabaena and Nostoc are more sensitive to the effects of the proposed strain and discoloration of their cells occurs within 2 hours of joint strain VKPM B-9405 it is steverivonia. In both cases, the lysis of cells of algae confirmed by microscopic examination. Example 2. Definition-spectrum algaecide factor of the strain VKPM B-9405. Due to the fact that the measurement of the optical density of the mixture of seaweed Amphidinium, Prorocentrum, Thalassiosira strain VKPM B-9405 was not possible due to the high turbidity of the resulting suspensions, the estimation of the spectrum of its antagonistic (algaecide) actions carried out by registering the intensity of the colour mixtures of microalgae strain VKPM B-9405 occurring due to lysis of cells of algae. It should be noted that algae have different color. However, in all experiments was observed discoloration of the mixture of algae strain VKPM B-9405.
From table 3 it follows that all strains of algae are sensitive to algaecidal activity of the strain-9405, but the level of sensitivity is different. Example 3. Physico-chemical characteristics of algaecidal properties of the proposed strain Algaecidal activity of the supernatant liquid were grown according to standard methods strain VKPM B-9405 assessed in relation to the cells of the algae Nostoc. Registered by the change in optical density algaecidal activity remained when heating at 80°C for 10 minutes and the autoclave at the 0.8 atmospheres - 5 minutes and the action of proteolytic enzymes (table 4).
Example 4. Preventing the development of cyanobacteria Nostoc and Anabaena in the presence of strain VKPM B-9405 The possibility of preventing the development of algae, such as cyanobacteria Nostoc and Anabaena in the presence of the culture fluid of strain VKPM B-9405 (legislationi effect). In a conical flask with a volume of 20 ml was made by culture of cyanobacteria Nostoc or Anabaena and the culture fluid of strain VKPM B-9405 grown as described previously (in the ratio 9:1). In the control samples were added to the modified medium BG-11. Total volume of the mixture was 10 ml. When observing within 14-30 days of the growth and development of cyanobacteria in contact with the strain VKPM B-9405, did not occur. In the control flasks with visual OBS is Denia and by determining the optical density of the cultures was observed rapid growth of cyanobacteria (table 5).
Thus, it is shown to prevent the effect of the strain VKPM B-9405 on the development of cyanobacteria Nostoc and Anabaena. Example 5. The destruction of three-dimensional structures of cyanobacteria ("mats") Provided the possibility of "mats" in the laboratory, as well as the impact of the proposed strain VKPM B-9405 on the three-dimensional structure of the cyanobacteria Nostoc and Anabaena in high concentrations. In the process of further cultivation in Petri dishes 1.5 to 2-month-old cultures of strains of cyanobacteria grown in the described mode, 3-4 weeks observe the formation of "mats". In the experimental Petri dishes with the "mats" of cyanobacteria contribute ml of the culture fluid of the proposed strain VKPM B-9405 by a uniform spray over the entire surface. In the control cups make an equal volume (3 ml) modified medium BG-11. In experienced cups in 24 hours see changing to light yellow color three-dimensional structures ("mats") cyanobacteria in contact with the strain VKPM B-9405. In the control cups by visual observation does not change the color of cyanobacteria and "mats" were blue-green color. These data confirm that the strain VKPM B-9405 has a damaging effect on the three-dimensional structure ("mats"), formed by cyanobacteria Nostoc and Anabaena in the process of its development. References 1. Carmical, W.W. J. App. Bacteriol., 1992, C. 445 to 459. 2. Lahti, K., Ahtiainen, J., Rapalla, J., Sivonen, K., Niemela, S.I. Lett. App. Environ.,1995, T.21, 109-114. 3. M.Z. Alam, M. Otaki, H. Furumai, Ohgaki S. Water Res., 2001, t.35, 1008-1014. 4. C.Y. Ahn, M.H. Park, Joung S.H., Kim H.S., K.Y. Jang, Oh H.M.Environ Sci Technol., 2003, v.37 (13), 3031-3037. 5. C.Y. Ahn, Joung S.H., J.W. Jeon, H.S. Kim, Yoon B.D., Oh H.M.Biotechnol Lett., 2003 t, 1137-1142. 6. Yoshida T., Takashima Y, Tomaru Y, Shirai Y, Takao Y, Hiroishi S, Nagasaki K. Appl. Environ. Environ., 2006, t (2), 1239-1247. 7. Hibayashi R, Imamura N.J Antibiot (Tokyo), 2003, t (2), 154-159. 8. Stanier, R.Y., Kunisawa, R., Mandel, M., Cohen-Basire, G. Bacteriool. Rev. 1971, t.35 (2), 171-205. The strain of bacteria Brevibacillus laterosporus VKPM B-9405 suppressing and preventing the development of microscopic algae of different taxonomic types.
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