Method for evaluating functional liver condition |
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IPC classes for russian patent Method for evaluating functional liver condition (RU 2305844):
                      
 Method for selecting chronic cardiac insufficiency patients treatable with nebivolol / 2305498
Method involves determining endothelin-1 concentration in blood plasma. Its value being equal to 1.5-5 fmole/ml, treatment with Nebivolol is administered.
Method for differential diagnostics of purulent meningitis / 2305287
In spinal fluid (SF) one should detect the concentration of C-reactive protein (C-RP) and at its value being >2.9 mg/l it is possible to diagnose meningococcal meningitis, at SF C-RP value being ≤2.9 mg/l - nonmeningococcal meningitis. Application of the present method provides the chance to increase accuracy, specificity and information value of differential diagnostics of purulent meningococcal and non-meningococcal meningitis that enables to carry out earlier specific treatment. The method in question, moreover, is very quick and simple in implementation.
Method for evaluating the severity of tuberculosis infection / 2305286
In the experiment upon guinea pigs in blood plasma one should detect the concentration of taurine and glutamic acid and at taurine concentration being below 68 mcM/l and glutamic acid being below 76 mcM/l it is necessary to evaluate the state as severe, at taurine concentration ranged 68-89 mcM/l and that of glutamic acid ranged 125-227 mcM/l - as average severity degree, and at taurine and glutamic acid concentrations being above 89 and 227 mcM/l, correspondingly - as light severity degree. Application of the present method enables to increase information value and significance of the method in question and, also, detect body reactivity according to the state of its different systems.
 Method for predicting diabetic retinopathy / 2304786
Due to biochemical testing lacrimal fluid (LF0 it is necessary to detect the concentration of cholesterol, activity of superoxide dismutase (SOD) and catalase, in blood one should detect the concentration of nitrites and based upon the values obtained calculate Function 1 and Function 2. Function 1 = -3.4+0.29(NO2)+32.5(cholesterol)-0.97(catalase)-17.28(SOD) - corresponds to Y-axis values and Function 2 = -7.14+0.17(NO2)+37.2(cholesterol)+2.1(catalase)-17.28(SOD) - corresponds to X-axis values on the scheme to detect lesion type. If the point obtained is on the field of the 1st scheme one should detect the presence of nonproliferative DR form in a patient at no risk of proliferation; if it is on the field of the 2nd scheme - nonproliferative DR form with the risk of proliferation; if it is on the field of the 3d scheme - proliferative form of retinopathy in the phase of neovascularization. Application of the present method enables to carry out diagnostics in due time, differentiation in doubtful cases directed towards adequate treatment and decreasing the risk for DR progressing.
Method for predicting the development of postgastroresectional peptic ulcers / 2304785
The present innovation deals with predicting the development of postgastroresectional peptic ulcers in patients due to detecting the level of gastrin after stimulation, pepsinogen, prostaglandins PGE2 and PG F2α, correspondingly, in mucosa of gastric stump and in mucosa of gastroenteroanastomosis. At increasing gastrin level up to 12.5 pM/l and higher, that of pepsinogen up to 10.0 mcg/l and higher and decreasing the level of prostaglandin PGE2 up to 570 and lower and at the decrease of prostaglandin PG F2α level up to 652 ng/g and lower one should predict the development of postgastroresectional peptic ulcer. Application of the present innovation enables to increase the accuracy in predicting the development of postgastroresectional peptic ulcers.
Method for predicting the chance for osteomyelitis chronization in children / 2304784
During the moment of exacerbation and on ending the disease in question one should detect the content of alpha-1 proteinase inhibitor and alpha-1 acid glycoprotein in blood due to immunoturbidimetric technique. At decreased content of alpha-1 proteinase inhibitor from 2.3-2.6 g/l up to 1-1.2 g/l, and alpha-1 acid glycoprotein from 2.4-2.6 g/l up to 0.7-0.8 g/l one should predict the absence of osteomyelitis chronization. At decreased content of alpha-1 proteinase inhibitor from 2.9-3.1 g/l up to 1.8-1.6 g/l, and that of alpha-1 acid glycoprotein from 3-3.2 g/l up to 1.2-1.3 g/l one should predict osteomyelitis chronization. Application of the present method enables to predict the disease flow in shorter terms at high significance that provides the chance to prescribe the complex of efficient measures in due time which vary depending upon the disease flow.
Method for predicting the development of gestosis / 2304783
Starting from 22nd wk of pregnancy it is necessary to detect the concentration of neurospecific enolase and glyofibrous acid protein in blood serum. The development of gestosis in pregnant women should be predicted by the value of neurospecific enolase being above 12.4 ng/ml and glyofibrous acid protein being above 4 ng/ml. Moreover, severe gestosis should be predicted at the value of neurospecific enolase being 19 ng/ml and higher and glyofibrous acid protein being 10 ng/ml and higher. Application of the method enables to increase the accuracy of prediction.
Method for evaluating the results of surgical sanitation of inflammation focus in patients with chronic osteomyelitis / 2304781
The present innovation deals with laboratory investigations in case of traumatological pathology. One should simultaneously carry out microscopic investigation of material sample out of inflammation focus and blood from cubital vein to study the growth of microorganisms in the focus and in case of its absence and at registering positive index of digestion in blood with test culture S.aureus 209 P inside neutrophil after incubation, the value of IgM being 0.5-1.3 against the norm, the value of C-reactive protein being up to 12.0 mg/l it is possible to conclude upon satisfactory result of therapy conducted. Application of the present method enables to evaluate the degree of inflammatory process in bony tissue and, also, the state of phagocytic function of neutrophils as the first line of protection against microbial invasion.
Method for predicting the frequency of acute respiratory-viral infections due to evaluating the state of local immunity in children of primary-school age / 2303784
One should detect gamma-glutamyl transferase (GGT) in saliva sampled in the morning to an empty stomach. At GGT values being under 0.85 IU/l or above 3.4 IU/l it is possible to diagnose the decrease of local immunity in children and predict the risk for transferring a healthy child into the group of frequently sick patients. Application of the present method enables to predict the frequency of acute respiratory-viral infections (ARVI) before the procedure of prophylactic vaccinations, provides the chance for carrying out corresponding correction measures. The innovation is simple in application being noninvasive and could be applied in any age group.
 Method for quantitative determination of antibodies concentration specific to human endometrial tissue stromal cells antigen and in human biological fluid containing specific antibodies / 2303267
Method involves using the usual solid-phase enzyme immunoassay based on chemical binding antigen with microplate surface followed by placing antibodies-containing biological fluid on microplate, incubation, carrying out the color reaction and spectrophotometric evaluation of the color reaction indices. The end antigen is prepared by enzymatic hydrolysis of human normal tissue samples containing stromal cells of endometrial tissue. The enzymatic hydrolysis reaction is terminated by addition of chelating agent. Prepared mixed suspension of cells is centrifuged in continuous density gradient resulting isolation of stromal cells. Microsomal fraction of stromal cells is used as the end antigen that is diluted and bound covalently with microplate well surface. Then calibrating material prepared on basis of standard preparation antibodies specific to the end antigen is added into wells set, and biological fluid samples for analysis are added into another wells set. After carrying out the usual solid-phase enzyme immunoassay and using spectophotometry data a calibrating curve is plotted and the concentration of specific antibodies is determined using this curve. Invention provides the development of method for assay of antibodies amount specific to endometrial tissue stromal cells exceptionally that gives possibility for clinical diagnosis of autoimmune syndrome. Invention can be used in gynecology and obstetrics for diagnosis of autoimmune syndrome accompanying to clinical picture of endometriosis and other diseases in female reproductive system.
 Method for predicting the character of bacterial keratitis flow / 2245553
In lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula:
Method for detecting the sequence of applied lesions / 2245555
For the purpose to detect the sequence of applied lesions at availability of several wounds, scratches and ecchymoses on a cadaver one should study the activity of alkaline peptides isolated out of affected tissue by the impact of blood neutrophils of healthy donors upon phagocytosis. Moreover, the highest stimulating effect belongs to the peptides isolated out of the lesion applied earlier. The method enables to detect the sequence of applied lesions more accurately and differentiate the repeated lesion applied 5 min later, or more.
Method for biochemical detecting the degree of chronic hepatitis activity / 2246112
In blood serum one should detect the level of lactoferrin and biliary acids. At their ratio being equal to 5-17 it is necessary to detect chronic hepatitis of high activity.
 Early diagnosis method for diagnosing external genital endometriosis in women / 2247391
Method involves determining cathepsin D activity in endometrium bioptate. The value being equal to or less than 0.1 units of enzymatic activity per hour, external genital endometriosis is diagnosed.
Method for estimating enteric detoxication in the cases of generalized peritonitis / 2247392
Method involves studying lactoferrin content in blood serum and peritoneal exudates in postoperative period every day during the first three days. Lactoferrin concentration in blood serum being concurrently reduced by 0.02 mcmole/l or less and increasing lactoferrin concentration in peritoneal exudates by 0.04 mcmole/l or more, enteric detoxication is considered to be effective.
Method for diagnosing septic process and predicting septic complications development in children / 2248572
Method involves determining plasminogen/plasmin, α2-macro-globulin, α1-antitripsin content at the first, third, fifth and tenth day. The plasminogen/plasmin level being equal to 66-74 mcmole/l or 100-120 mcmole/l, α2-macro-globulin level of 2.7-3.0 mcmole/l, α1-antitripsin content of 2.38-3.2 mcmole/l, systemic inflammatory response to purulent infection, light severity degree endotoxicosis is diagnosed and favorable disease outcome is predicted. The plasminogen/plasmin level being equal to 50-65 mcmole/l or 125-160 mcmole/l, α2-macro-globulin level of 2.3-2.6 mcmole/l, α1-antitripsin content of 3.3-4.0 mcmole/l, sepsis with organ and system dysfunction, moderate severity degree endotoxicosis is diagnosed and septic complication availability and lingering disease development course is predicted. The plasminogen/plasmin level being equal to 39-40 mcmole/l, α2-macro-globulin level of 1.58-2.08 mcmole/l, α1-antitripsin content of 5.0-6.2 mcmole/l, severe sepsis, septic shock, severe degree endotoxicosis is diagnosed and unfavorable disease outcome is predicted.
 Method for detecting oxidized tryptophan metabolites at endogenic intoxication / 2249219
At testing one should precipitate high-molecular compounds with acetonitrile and register supernatant's spectral characteristics. Supernatant should be applied onto a paper filter, dried and put into solution containing aromatic aldehyde, acetone and concentrated hydrochloric acid taken at weight ratio of 70:5:1 to be kept for 2-3 min. Then it should be once again dried up to detect qualitative and semiquantitative content of oxidized tryptophan metabolites by intensity and chromatic shades. Moreover, by chromatic shades of yellow dyeing it is possible to detect the content of hydroxylated metabolites and by chromatic shades of violet dyeing - that of unhydroxylated ones.
Method for predicting unfavorable result of metastatic peritonitis / 2251700
In patients one should study the content of lactoferrin in peritoneal exudates during the 1st d of postoperational period and at decreased value being below 3500 ng/ml on should predict unfavorable result. The suggested method provides correction of possible postoperational complications that deteriorate the flow of peritonitis and lead to lethal result.
Method for diagnosing endotoxicosis condition in cows suffering from acute pyocatarrhal endometritis / 2252418
Method involves determining low and middle molecular mass substances content in blood plasma and erythrocytes and general blood plasma albumin concentration. Integral index is calculated on basis of obtained values using formula II=100*S238-298(plasma)/S238-298(erythrocytes)*GAC, where S238-298(plasma) and S238-298(erythrocytes) are the low and middle molecular mass substances content in blood plasma and erythrocytes, respectively, determined from area of figures restricted by spectral curves in wavelength range of 238-298 nm and abscissa axis (conditional units2); GAC is the general blood plasma albumin concentration (g/l). The value being from 2.1 to 3.0, the first endotoxicosis degree is diagnosed. The value being from 3.1 to 4.5, the second endotoxicosis degree is diagnosed. The value being from 4.5 to 6.0, the third endotoxicosis degree is diagnosed. The value being greater than 6.0, the fourth endotoxicosis degree is diagnosed. The normal value is equal to 0.5-2.0.
Method for evaluating inflammatory process activity in infantine osteomyelitis cases / 2252419
Method involves separating blood serum proteins into fractions, determining albumins and alpha-2-globulins content and controlling their content changes during the disease development process. Gamma-globulin content is determined in per cent ratio with respect to total protein quantity. Then, changes in the fractions content are controlled from the first to the third week. Albumin content being in norm and alpha-2-globulins content becoming greater to the end of the first week by 30-50% when compared to normal value and dropping to norm at the second week end and gamma-globulin content increasing from norm by 10-30% to the second or the third week, high inflammatory process activity is to be diagnosed. Albumin content dropping by 10-30% from normal value at the second week, alpha-2-globulins content growing by 10-20% of norm and gamma-globulin content dropping by 30-50% at the second or the third week when compared to norm, low inflammatory process activity is to be diagnosed.
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FIELD: veterinary medicine. SUBSTANCE: method involves determining α-globulin, albumin, β-globulin concentrations and calculating diagnostic criterion value as newborn cattle calves functional liver state coefficient from formula of Kfsl=A/(A+B+C), where A is the α-globulins quantity, B is the albumins quantity and C is the β-globulins quantity. Coefficient Kfsl value being <0.2, functional hepatic insufficiency is diagnosed. EFFECT: high accuracy in evaluating functional liver state. 3 dwg, 3 tbl
The present invention relates to veterinary medicine and can be used in normal and pathological physiology, clinical diagnosis and therapy. There are different ways of assessing the functional state of the liver [EN 2194280 C2, 7 G01N 33/48, 10.12.2002; EN 2089914 C1, 6 G01N 33/68, 09.10.1997], but none of them can be used to assess the functional state of the liver of the newborn calves cattle. Closest to the claimed method is used to diagnose hepatosis in cows [EN 2029304 C1, 6 G01N 33/04, 02.20.1995]. However, it cannot be used to assess the condition of the liver of newborn calves cattle, as it can be applied only for the diagnosis of hepatosis in cows. The essence of it is that the functional state of the liver judged by the content of urea in milk and at the proper rate to 4,06 mmol/l and the presence of clinical signs of hypotension stomach, increasing the area of liver dullness (rear border of the liver for the thirteenth rib pain liver percussion - diagnose on steatosis. Our proposed method has a number of design differences, allowing to minimize the risk of complications and to make possible the evaluation of the functional state of the liver of the newborn calves cattle IC is the same. Manifestations of various diseases of the liver are the traits of non-specificity, therefore, only a set of studies to address issues of etiology and nosology of belonging pathological process, activity and functional status of the liver. In veterinary practice, the study of the liver mainly produce common methods, only if necessary, resorting to special. Of the common methods often use inspection, palpation and percussion. Laboratory diagnosis uses many indicators of the functional state of the liver, but not all of them convenient to use in assessing the extent of damage and predicting the outcome of diseases of the hepatobiliary system in the newborn of young cattle. The body of the newborn is characterized by immaturity of many organs and systems to maintain homeostasis, which complicates the use routine means of clinical and laboratory diagnostics, and the interpretation of the majority of laboratory tests has its own characteristics. Our studies have established a pattern of change of protein metabolism in calves in the first two weeks of life in violation of the functional state of the liver. Protein metabolism in newborns character is by preferential catabolism colostral derived protein and a low level of synthesis of its own, however, their qualitative composition diversity except gamma globulin fraction, it mainly presents proteins of hepatic origin. It was shown that calves differ in the number and dynamics of alpha-globulin fraction of proteins. A special place in it belongs to the alpha-1-antitripsin. Forming in the liver (80-90% of all alpha-1-globulin), it is the main inhibitor of leukocyte elastase and other cathepsins (tissue proteases). While reducing its synthesis, which is common in premature and physiologically immature young, violated the reparative processes in the liver and lungs. These phenomena are often complicated by the hard place pneumonia and liver diseases of the newborn, progressing to cirrhosis. For veterinary practices significant problem is the determination of the amount of this serum component, since the procedure requires species-specific sera. We proposed to evaluate the content of alpha-1-antitrypsin in the serum of newborn calves on indirect indicators, based on the provision that up to two weeks of age alpha-2-globulin fraction is not allocated fractionation of proteins, due to the immaturity of the immune system. Therefore, electrophoretic defined FR the Ktsia alpha-globulin consists of components alpha-1. The task of the invention is a solution to the problem of estimation of the functional state of the liver of newborn calves cattle. The essence of the invention lies in the fact that to determine the number of alpha-globulin, albumin, beta-globulin and blood by the formula determine the diagnostic criterion is the ratio of the functional state of the liver of newborn calves cattle (KFund):
where a is the number of alpha-globulin, In the amount of albumin, the amount of beta-globulins, when the magnitude of the coefficient of the functional state of the liver of newborn calves cattle (KFund)≥2 functional state of the liver is considered satisfactory (animal normotrophic), when the value of the coefficient of the functional state of the liver of newborn calves cattle (KFund)<0.2 to ascertain the functional insufficiency of the liver (animal hypotrophic). The example run. The method used by 47 calves black-motley breed belonging to echazu Belgische "Central". At the time of the experiment all animals were kept in individual cages, feeding and maintenance typical for this sector. All studies were conducted in winter-spring seasons (1998/1999, 1999/2000 and 200/2001,), in the period of the greatest stress homeostatic systems of the animal body. Clinical, hematological and biochemical studies calves were organized every 3-4 days, from birth to two weeks of age. Clinical studies of calves was carried out according to the standard scheme with a description of all changes in curatione leaves. Material for laboratory studies in all cases were collected in the period of physiological rest, before the morning feeding. Laboratory blood tests were conducted not later than 5-6 hours after selection of the material. In our research we divide the youngsters into two groups: clinically healthy calves (normotrophic) and lipotropics (with impaired functional status of the liver). The data obtained show that during the reporting period, from 47 calves 17 (36.2 percent) are born with severe disabilities clinical indicators. So, body weight at ypotrofies calves at birth accounted for 24.5±0.89 kg vs. 27.4±0,66 kg in clinically healthy calves (p<0,02). Low body weight at birth was accompanied by the following changes: the skin is dry, easy to assemble folds; subcutaneous fat layer is not developed; the coat dull, badly kept by mechanical action; eyeballs sunken; the visible mucous membranes of cyanotic; breathing uneven (frequency 2± 2 min), surface; cardiac impulse strengthened (pulse 120±10 per minute, weak filling). Data from laboratory tests by age intervals are presented in tables 1-3, and as a result, the calves were divided into two groups: clinically healthy calves (normotrophic) (Fig 1. Calf liver. Paint hematoxylin and eosin. The lens 20, the eyepiece 7) and lipotropics (with impaired functional status of the liver). Found that in animals with symptoms of malnutrition and biochemical changes characteristic of functional immaturity of the liver (Hypo - and dysproteinemia, when a critically low level of alpha-globulin fraction, see tables 1-3), it was also noted violations of the architecture of the liver tissue. In General, these changes were limited to nedefiniranom hepatic beams, a sharp decrease (or disappearance) of the number of binucleate hepatocytes and the presence of destructive fields modified cells phenomena karyolysis and macrophage reaction. Also marked nuclear polymorphism, the presence of hyperchromic nuclei in hepatocytes foci of micro - and micronecrosis, cytolysis and karyolysis in hepatocytes, phenomena karyopyknosis. (figure 2. The focus of necrosis. Calf liver. Paint hematoxylin and eosin. The lens 40, the eyepiece 7). One of the calves detected changes, typical for the initial stage of cirrhotics the CSOs rebirth of the body (figure 3. Extensive necrosis in the parenchyma, the phenomenon of hyalinization necrotic masses of lymphoid infiltration of the hearth, cytolysis and karyolysis in hepatocytes, phenomena karyopyknosis. Calf liver. Paint hematoxylin and eosin. The lens 40, the eyepiece 7). Moreover, the degree of these changes was the higher, the smaller was ToFund.
Thus, the method call is employed to expand the range of tests of clinical Hepatology and increase the efficiency of diagnostic procedures, and also contributes to the prediction of the outcome of the hepatobiliary system pathology in the newborn of young cattle. The method is easy to use and requires no special skills, can be used to conduct research on issues and neonatal metabolic pathology. Sources of information 1. EN 2194280 C2, 7 G01N 33/48, 10.12.2002; 2. EN 2089914 C1, 6 G01N 33/68, 09.10.1997; 3. EN 2029304 C1, 6 G01N 33/04, 02.20.1995. The method of assessing the functional state of the liver, including the statement of functional test, characterized in that to determine the number of alpha-globulin, albumin, beta-globulins of blood in the first 14 days. after birth and the formula to determine the diagnostic criterion is the ratio of the functional state of the liver of newborn calves cattle (KFund):
where a is the number of alpha-globulin, In the amount of albumin, the amount of beta-globulins, when the magnitude of the coefficient of the functional state of the liver of newborn calves cattle (KFund)≥0,2 functional state of the liver are considered satisfactory when the value of the coefficient of the functional state of the liver of newborn calves cattle (KFund)<0.2 to ascertain functional not tecnost liver.
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