Method for differential diagnostics of purulent meningitis |
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IPC classes for russian patent Method for differential diagnostics of purulent meningitis (RU 2305287):
Method for evaluating the severity of tuberculosis infection / 2305286
In the experiment upon guinea pigs in blood plasma one should detect the concentration of taurine and glutamic acid and at taurine concentration being below 68 mcM/l and glutamic acid being below 76 mcM/l it is necessary to evaluate the state as severe, at taurine concentration ranged 68-89 mcM/l and that of glutamic acid ranged 125-227 mcM/l - as average severity degree, and at taurine and glutamic acid concentrations being above 89 and 227 mcM/l, correspondingly - as light severity degree. Application of the present method enables to increase information value and significance of the method in question and, also, detect body reactivity according to the state of its different systems.
 Method for predicting diabetic retinopathy / 2304786
Due to biochemical testing lacrimal fluid (LF0 it is necessary to detect the concentration of cholesterol, activity of superoxide dismutase (SOD) and catalase, in blood one should detect the concentration of nitrites and based upon the values obtained calculate Function 1 and Function 2. Function 1 = -3.4+0.29(NO2)+32.5(cholesterol)-0.97(catalase)-17.28(SOD) - corresponds to Y-axis values and Function 2 = -7.14+0.17(NO2)+37.2(cholesterol)+2.1(catalase)-17.28(SOD) - corresponds to X-axis values on the scheme to detect lesion type. If the point obtained is on the field of the 1st scheme one should detect the presence of nonproliferative DR form in a patient at no risk of proliferation; if it is on the field of the 2nd scheme - nonproliferative DR form with the risk of proliferation; if it is on the field of the 3d scheme - proliferative form of retinopathy in the phase of neovascularization. Application of the present method enables to carry out diagnostics in due time, differentiation in doubtful cases directed towards adequate treatment and decreasing the risk for DR progressing.
Method for predicting the development of postgastroresectional peptic ulcers / 2304785
The present innovation deals with predicting the development of postgastroresectional peptic ulcers in patients due to detecting the level of gastrin after stimulation, pepsinogen, prostaglandins PGE2 and PG F2α, correspondingly, in mucosa of gastric stump and in mucosa of gastroenteroanastomosis. At increasing gastrin level up to 12.5 pM/l and higher, that of pepsinogen up to 10.0 mcg/l and higher and decreasing the level of prostaglandin PGE2 up to 570 and lower and at the decrease of prostaglandin PG F2α level up to 652 ng/g and lower one should predict the development of postgastroresectional peptic ulcer. Application of the present innovation enables to increase the accuracy in predicting the development of postgastroresectional peptic ulcers.
Method for predicting the chance for osteomyelitis chronization in children / 2304784
During the moment of exacerbation and on ending the disease in question one should detect the content of alpha-1 proteinase inhibitor and alpha-1 acid glycoprotein in blood due to immunoturbidimetric technique. At decreased content of alpha-1 proteinase inhibitor from 2.3-2.6 g/l up to 1-1.2 g/l, and alpha-1 acid glycoprotein from 2.4-2.6 g/l up to 0.7-0.8 g/l one should predict the absence of osteomyelitis chronization. At decreased content of alpha-1 proteinase inhibitor from 2.9-3.1 g/l up to 1.8-1.6 g/l, and that of alpha-1 acid glycoprotein from 3-3.2 g/l up to 1.2-1.3 g/l one should predict osteomyelitis chronization. Application of the present method enables to predict the disease flow in shorter terms at high significance that provides the chance to prescribe the complex of efficient measures in due time which vary depending upon the disease flow.
Method for predicting the development of gestosis / 2304783
Starting from 22nd wk of pregnancy it is necessary to detect the concentration of neurospecific enolase and glyofibrous acid protein in blood serum. The development of gestosis in pregnant women should be predicted by the value of neurospecific enolase being above 12.4 ng/ml and glyofibrous acid protein being above 4 ng/ml. Moreover, severe gestosis should be predicted at the value of neurospecific enolase being 19 ng/ml and higher and glyofibrous acid protein being 10 ng/ml and higher. Application of the method enables to increase the accuracy of prediction.
Method for evaluating the results of surgical sanitation of inflammation focus in patients with chronic osteomyelitis / 2304781
The present innovation deals with laboratory investigations in case of traumatological pathology. One should simultaneously carry out microscopic investigation of material sample out of inflammation focus and blood from cubital vein to study the growth of microorganisms in the focus and in case of its absence and at registering positive index of digestion in blood with test culture S.aureus 209 P inside neutrophil after incubation, the value of IgM being 0.5-1.3 against the norm, the value of C-reactive protein being up to 12.0 mg/l it is possible to conclude upon satisfactory result of therapy conducted. Application of the present method enables to evaluate the degree of inflammatory process in bony tissue and, also, the state of phagocytic function of neutrophils as the first line of protection against microbial invasion.
Method for predicting the frequency of acute respiratory-viral infections due to evaluating the state of local immunity in children of primary-school age / 2303784
One should detect gamma-glutamyl transferase (GGT) in saliva sampled in the morning to an empty stomach. At GGT values being under 0.85 IU/l or above 3.4 IU/l it is possible to diagnose the decrease of local immunity in children and predict the risk for transferring a healthy child into the group of frequently sick patients. Application of the present method enables to predict the frequency of acute respiratory-viral infections (ARVI) before the procedure of prophylactic vaccinations, provides the chance for carrying out corresponding correction measures. The innovation is simple in application being noninvasive and could be applied in any age group.
 Method for quantitative determination of antibodies concentration specific to human endometrial tissue stromal cells antigen and in human biological fluid containing specific antibodies / 2303267
Method involves using the usual solid-phase enzyme immunoassay based on chemical binding antigen with microplate surface followed by placing antibodies-containing biological fluid on microplate, incubation, carrying out the color reaction and spectrophotometric evaluation of the color reaction indices. The end antigen is prepared by enzymatic hydrolysis of human normal tissue samples containing stromal cells of endometrial tissue. The enzymatic hydrolysis reaction is terminated by addition of chelating agent. Prepared mixed suspension of cells is centrifuged in continuous density gradient resulting isolation of stromal cells. Microsomal fraction of stromal cells is used as the end antigen that is diluted and bound covalently with microplate well surface. Then calibrating material prepared on basis of standard preparation antibodies specific to the end antigen is added into wells set, and biological fluid samples for analysis are added into another wells set. After carrying out the usual solid-phase enzyme immunoassay and using spectophotometry data a calibrating curve is plotted and the concentration of specific antibodies is determined using this curve. Invention provides the development of method for assay of antibodies amount specific to endometrial tissue stromal cells exceptionally that gives possibility for clinical diagnosis of autoimmune syndrome. Invention can be used in gynecology and obstetrics for diagnosis of autoimmune syndrome accompanying to clinical picture of endometriosis and other diseases in female reproductive system.
Method for predicting atrial fibrillation in cardiac ischemia patients / 2303266
Method involves applying immunoenzyme assay approach for analyzing blood for Chlamydia pneumoniae presence. Levels of inflammatory markers like C-reactive protein and tumor necrosis factor alpha are quantitatively determined. Seropositive result being the case with CRP level above 1.8 mg/l and TNF-α level above 2.4 pg/dl, atrial fibrillation occurrence is to be predicted.
Method for controlling climacteric disorders hormone replacement therapy safety in women in menopause period / 2303265
Method involves analyzing blood serum for alpha-2-glycoprotein associated with pregnancy taking concentration determined before administering hormone therapy for individual reference value and then, blood serum is analyzed once every half-year during the whole hormone replacement therapy application period. Alpha-2-glycoprotein associated with pregnancy having unchanged concentration or its concentration being increased by 50% when compared to the individual reference value, hormone replacement therapy application is to be continued. The concentration growing more than 50% high, when compared to the individual reference value, hormone replacement therapy application is to be prohibited.
 Method for predicting the character of bacterial keratitis flow / 2245553
In lacrimal liquid one should detect the content of interleukin 8 (IL-8) and that of interleukin 1 beta (IL-1β) to calculate prognostic coefficient (PC) due to dividing the first value by the second one by the following formula:
Method for detecting the sequence of applied lesions / 2245555
For the purpose to detect the sequence of applied lesions at availability of several wounds, scratches and ecchymoses on a cadaver one should study the activity of alkaline peptides isolated out of affected tissue by the impact of blood neutrophils of healthy donors upon phagocytosis. Moreover, the highest stimulating effect belongs to the peptides isolated out of the lesion applied earlier. The method enables to detect the sequence of applied lesions more accurately and differentiate the repeated lesion applied 5 min later, or more.
Method for biochemical detecting the degree of chronic hepatitis activity / 2246112
In blood serum one should detect the level of lactoferrin and biliary acids. At their ratio being equal to 5-17 it is necessary to detect chronic hepatitis of high activity.
 Early diagnosis method for diagnosing external genital endometriosis in women / 2247391
Method involves determining cathepsin D activity in endometrium bioptate. The value being equal to or less than 0.1 units of enzymatic activity per hour, external genital endometriosis is diagnosed.
Method for estimating enteric detoxication in the cases of generalized peritonitis / 2247392
Method involves studying lactoferrin content in blood serum and peritoneal exudates in postoperative period every day during the first three days. Lactoferrin concentration in blood serum being concurrently reduced by 0.02 mcmole/l or less and increasing lactoferrin concentration in peritoneal exudates by 0.04 mcmole/l or more, enteric detoxication is considered to be effective.
Method for diagnosing septic process and predicting septic complications development in children / 2248572
Method involves determining plasminogen/plasmin, α2-macro-globulin, α1-antitripsin content at the first, third, fifth and tenth day. The plasminogen/plasmin level being equal to 66-74 mcmole/l or 100-120 mcmole/l, α2-macro-globulin level of 2.7-3.0 mcmole/l, α1-antitripsin content of 2.38-3.2 mcmole/l, systemic inflammatory response to purulent infection, light severity degree endotoxicosis is diagnosed and favorable disease outcome is predicted. The plasminogen/plasmin level being equal to 50-65 mcmole/l or 125-160 mcmole/l, α2-macro-globulin level of 2.3-2.6 mcmole/l, α1-antitripsin content of 3.3-4.0 mcmole/l, sepsis with organ and system dysfunction, moderate severity degree endotoxicosis is diagnosed and septic complication availability and lingering disease development course is predicted. The plasminogen/plasmin level being equal to 39-40 mcmole/l, α2-macro-globulin level of 1.58-2.08 mcmole/l, α1-antitripsin content of 5.0-6.2 mcmole/l, severe sepsis, septic shock, severe degree endotoxicosis is diagnosed and unfavorable disease outcome is predicted.
 Method for detecting oxidized tryptophan metabolites at endogenic intoxication / 2249219
At testing one should precipitate high-molecular compounds with acetonitrile and register supernatant's spectral characteristics. Supernatant should be applied onto a paper filter, dried and put into solution containing aromatic aldehyde, acetone and concentrated hydrochloric acid taken at weight ratio of 70:5:1 to be kept for 2-3 min. Then it should be once again dried up to detect qualitative and semiquantitative content of oxidized tryptophan metabolites by intensity and chromatic shades. Moreover, by chromatic shades of yellow dyeing it is possible to detect the content of hydroxylated metabolites and by chromatic shades of violet dyeing - that of unhydroxylated ones.
Method for predicting unfavorable result of metastatic peritonitis / 2251700
In patients one should study the content of lactoferrin in peritoneal exudates during the 1st d of postoperational period and at decreased value being below 3500 ng/ml on should predict unfavorable result. The suggested method provides correction of possible postoperational complications that deteriorate the flow of peritonitis and lead to lethal result.
Method for diagnosing endotoxicosis condition in cows suffering from acute pyocatarrhal endometritis / 2252418
Method involves determining low and middle molecular mass substances content in blood plasma and erythrocytes and general blood plasma albumin concentration. Integral index is calculated on basis of obtained values using formula II=100*S238-298(plasma)/S238-298(erythrocytes)*GAC, where S238-298(plasma) and S238-298(erythrocytes) are the low and middle molecular mass substances content in blood plasma and erythrocytes, respectively, determined from area of figures restricted by spectral curves in wavelength range of 238-298 nm and abscissa axis (conditional units2); GAC is the general blood plasma albumin concentration (g/l). The value being from 2.1 to 3.0, the first endotoxicosis degree is diagnosed. The value being from 3.1 to 4.5, the second endotoxicosis degree is diagnosed. The value being from 4.5 to 6.0, the third endotoxicosis degree is diagnosed. The value being greater than 6.0, the fourth endotoxicosis degree is diagnosed. The normal value is equal to 0.5-2.0.
Method for evaluating inflammatory process activity in infantine osteomyelitis cases / 2252419
Method involves separating blood serum proteins into fractions, determining albumins and alpha-2-globulins content and controlling their content changes during the disease development process. Gamma-globulin content is determined in per cent ratio with respect to total protein quantity. Then, changes in the fractions content are controlled from the first to the third week. Albumin content being in norm and alpha-2-globulins content becoming greater to the end of the first week by 30-50% when compared to normal value and dropping to norm at the second week end and gamma-globulin content increasing from norm by 10-30% to the second or the third week, high inflammatory process activity is to be diagnosed. Albumin content dropping by 10-30% from normal value at the second week, alpha-2-globulins content growing by 10-20% of norm and gamma-globulin content dropping by 30-50% at the second or the third week when compared to norm, low inflammatory process activity is to be diagnosed.
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FIELD: medicine, neurology, infectious diseases. SUBSTANCE: in spinal fluid (SF) one should detect the concentration of C-reactive protein (C-RP) and at its value being >2.9 mg/l it is possible to diagnose meningococcal meningitis, at SF C-RP value being ≤2.9 mg/l - nonmeningococcal meningitis. Application of the present method provides the chance to increase accuracy, specificity and information value of differential diagnostics of purulent meningococcal and non-meningococcal meningitis that enables to carry out earlier specific treatment. The method in question, moreover, is very quick and simple in implementation. EFFECT: higher efficiency and accuracy of differential diagnostics. 3 ex
The invention relates to medicine, namely, neurology, infectious diseases, and can be used for the diagnosis of purulent meningitis. A known method for the diagnosis of purulent and serous meningitis on the basis of determining the level of C-reactive protein in the cerebrospinal fluid (Lobos M. et al. The Usefulness of establishing chosen acute phase proteins concentrations in serum and cerebrospinal fluid for differential diagnosis and monitoring of purulent meningitis in adults. Przegl Epidemiol. 1994; 48(3): 191-6). The disadvantages of the prototype: indicative aviakompaniiyu. The invention is aimed at solving problems: increasing the specificity and informative diagnosis of purulent meningitis. Technical result: acceleration diagnosis of purulent meningitis with calculation of C-reactive protein (C-RP) in the cerebrospinal fluid, allowing to differentiate purulent meningococcal and meminimalkan meningitis and to have the possibility of earlier specific treatment. The task is achieved by determining the level of C-reactive protein (C-RP) in cerebrospinal fluid (CSF) upon admission to the hospital. Depending on the values of p-SPM determine the etiology of meningitis. Criterion offer value-RP cerebrospinal fluid 2.9 mg/L. When the value of C-RP CSF above 2,9 mg/l diagnosed with meningococcal meningitis, when the value of C-RP CSF ≤ ,9 mg/l - aminimoaby meningitis. When the value of C-RP CSF above 2,9 mg/l diagnosed with meningococcal meningitis, when the value of C-RP CSF ≤ 2,9 mg/l aminimoaby meningitis. The method is as follows: the patient when admitted to hospital with suspected meningitis aseptic conditions during lumbar puncture take 1 ml of cerebrospinal fluid and determine the level of C-RP through immunoturbidimetric method at a wavelength of 340 nm. C-RP forms a precipitate with the specific antibodies contained in the test system. If turbidimetry determine the amount of bandwidth monochromatic light in the solution, the turbidity of which is determined by the formation of specific complexes of antigen-antibody. The rate of change of the standard solution is fixed by automatic analyzer. The time from the time of collection of samples to get the final result - a few hours. Examples of practical applications Example 1: patient D., 21, enrolled in the intensive care unit and intensive care unit in a serious condition with suspected meningitis. Objective: impaired consciousness to the level of deep spoor, rough meningeal symptoms, T - 39,5°With load and with poor hemorrhagic rash on the lower extremities. In the analysis of CSF expressed cellular protein dissociation. Test latex agglutination (antigens to the meningococcus) is positive.C-RP CSF - 5.4 mg/L. Ds: Meningococcal meningitis (mixed form of meningococcal infection). Assigned to penicillin in a daily dose of 300 mg/kg On the background of treatment with penicillin condition with fast positive dynamics. Discharged home on the 25th day in a state of clinical recovery. Example 2: Patient B., 19 years old, was admitted to the intensive care unit and intensive care unit in a serious condition with suspected meningitis. Objective: shallow spoor, T - 38,8°C, coarse meningeal symptoms, rash no. In the analysis of CSF cell protein dissociation. C-RP CSF - 1.2 mg/L. Test latex agglutination (antigens to the meningococcus) is negative. Ds: Aminimoaby purulent meningitis. Assigned Cefotaxime 6.0 g per day. On the background of treatment with Cefotaxime condition with fast positive dynamics. Discharged home on the 31st day in a state of clinical recovery. Example 3: Patient P., 31 years old, was admitted to the intensive care unit and intensive care unit in a serious condition with suspected meningitis. Objectively: in the mind, sharply estensione, T - 39,0°C, coarse meningeal symptoms, rash no. In the analysis of CSF expressed cellular protein dissociation. C-RP CSF of 4.1 mg/L. Ds: Meningococcal meningitis. N is meant penicillin in a daily dose of 300 mg/kg Bacteriological examination of blood and cerebrospinal fluid - the growth of pathogenic organisms is not received. On the background of treatment with penicillin condition with fast positive dynamics. Discharged home on day 21 in a state of clinical recovery. The positive effect of the proposed method consists in the following: the way vasocontraction at high speed, allows to differentiate meningococcal and meminimalkan purulent meningitis in the day of admission the patient may be estimated in the absence of microbiological confirmation of diagnosis is useful for monitoring the adequacy of therapy, and also allows you to exclude unjustified use of expensive antibiotics. The method of differential diagnosis of purulent meningitis by definitions in the cerebrospinal fluid (CSF) concentrations of C-reactive protein (C-RP), characterized in that when the value of > 2,9 mg/l diagnosed with meningococcal meningitis, when the value of C-RP CSF ≤ 2,9 mg/l aminimoaby meningitis.
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