Method for predicting diabetic retinopathy |
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IPC classes for russian patent Method for predicting diabetic retinopathy (RU 2304786):
  
Method for predicting therapeutically resistant depressions in case of endogenous psychic diseases / 2298190
The present innovation deals with detecting the resistance to antidepressant-containing therapy in patients with bipolar affective disorders and recurrent depressive disorders. One should detect the degree of depression by Hamilton's scale, calculate leukocytic formula of blood clinic analysis, detect quantitative content of phosphatidylethanolamines, sphingomyelins and phosphatidylcholines in whole blood to calculate the content values of these phospholipids. Therapeutically resistant depressions in patients with endogenous psychic diseases should be predicted at the value of depressive symptomatics degree being 20.9±2.2 points, the value of phosphatidylethanolamines being 30.2±1.3%, sphingomyelins being 28.6±2.4% and phosphatidylcholines being 28.1±1.9% and the availability of monocytosis and lymphocytosis being above 5% and 25%, correspondingly.
 Method for differential predicting the complications of inborn femoral dislocation in children and teenagers / 2296337
It is necessary to analyze the results of clinical signs of the disease and roentgenological inspection in patients, and, additionally, in blood serum one should test a lipid profile - the content of total cholesterol, cholesterol of low-density lipoproteides (CS-LDLP), cholesterol of extremely low-density lipoproteides (CS-ELDLP) and triglycerides. One should calculate the integral coefficient (IC) by the following formula
Method for diagnosing cardiac insufficiency at early stage in hypothyroidism syndrome cases / 2292049
Method involves determining saturated and polyunsaturated fatty acids concentration in blood. Saturated to polyunsaturated fatty acids concentration ratio is calculated. The value being greater than 1, cardiac insufficiency is to be diagnosed.
 Method for predicting disease outcome in generalized purulent peritonitis cases / 2292048
Method involves determining general cholesterol, high density lipoprotein cholesterol, leukocytic intoxication index, leukocyte shift index. General cholesterol value falling below 2.5 mmole/l, high density lipoprotein cholesterol below 0.9, leukocytic intoxication index growing above 4.0 and leukocyte shift index above 6.0, unfavorable treatment outcome is to be predicted.
Method for diagnosis of course severity of ulcerous colitis / 2289819
Method involves assay of cystic bile biochemical composition and severe course of ulcerous colitis is diagnosed in case of the decreasing concentration of cholesterol to 0.5 mmole/l, bile acids to 1.5 mmole/l, phospholipids to 1.8 mmole/l as compared with normal value, decreasing cholate-cholesterol coefficient to 3 and lower, increasing Thomas-Hoffmann coefficient to 1.7 and above, Swell index to 7.7 and above, and Rubens index to 4.9 and above. Using the proposed method allows enhancing precision and information content of diagnosis of severe degree of ulcerous colitis.
 Method for evaluating thyroid dysfunction treatment effectiveness / 2289139
Method involves determining thyrotropic hormone (TTH), free thyroxin (FR T4), total cholesterol (TCHS) and β-lipoproteins (β-LP) concentration before and after treatment. Dimensionless integral biochemical index IE is calculated as
Method for differential diagnostics of gastrointestinal form of alimentary allergy in early- and pre-school-aged children / 2287166
In blood serum one should detect the content of pre-β-lipoproteides and β-lipoproteides. At the ratio of levels of pre-β-lipoproteides against β-lipoproteides being above 0.31 one should differentiate gastrointestinal form of alimentary allergy against another gastrointestinal pathology.
Method for predicting the development of gastrointestinal tract paresis in patients with vertebral traumas / 2286089
In patients with vertebral traumas for about 2-3 h after lesion one should simultaneously determine the level of hormone cortisol, malonic dialdehyde (MDA) in blood serum, and, also, the value of total bioelectric activity (TBEA) of gastrointestinal tract (GIT) and at cortisol values being 981.7 nM/l and higher, and MDA values - 8.35 mM/l and higher and values of TBEA being 387.75 mcV and lower it is possible to predict the development of GIT paresis.
Method for predicting treatment results in infiltrative pulmonary tuberculosis patients / 2284041
Method involves determining phosphatidyl choline (PhC) share and cardiolipine (CL) share in bronchoalveolar lavages. Phosphatidyl choline/cardiolipine (PhC/CL) coefficient is calculated. Unfavorable treatment results are predicted in cases of PhC/CL<0.5.
Method for diagnosing parodontitis severity degree / 2280874
Method involves determining prostaglandin E2, arachidonic acid and phosphatidyl inositols in blood sample taken from patient gingiva. The mentioned substance levels data are interpreted in terms of severe, medium or light stage of the disease.
Method for determining patient health state severity degree in the cases of inflammatory diseases of uterine appendages / 2302642
Method involves determining oxidation-reduction level of thiol compounds (SS and SH) in patient blood serum. Their thiol-disulfide equilibrium K= SH/SS is calculated. The values K=1.2-1.81, heavy patient health state is to be diagnosed. The values K=1.82-2.32, patient health state of moderate severity is to be diagnosed. The values K=2.33-3.4, satisfactory patient health state is to be diagnosed.
Method for predicting spermatogenesis stimulation effectiveness in oligospermic infertility (secretory olygo- and azoospermia) cases / 2302641
Method involves determining biochemical parameters in sperm. Nitrate level being found higher than 0.185 mmole/ml, quality and quantity of spermatogram values improvement is to be predicted.
Method for determining thyroid gland structural disorders / 2302640
Method involves determining iodine concentration in human hair. The value being less than 0.27 mcg/g, diffuse changes are diagnosed. The value being greater than 4.2 mcg/g, diffuse-nodular thyroid gland changes are diagnosed.
Method for estimating psoriatic erythrodermia treatment effectiveness / 2297004
Method involves determining acid alpha-1 glycoprotein and nitrogen oxide content at the time of disease exacerbation and at the moment the patient treatment being over. Acid alpha-1 glycoprotein content reduction being 1.5-1.6 times as high, and nitrogen oxide being 1.8-2.2 times as high, when compared to initial level, high treatment efficiency is considered to take place. Acid alpha-1 glycoprotein content reduction being 1.4-1.75 times as high, and nitrogen oxide being 1.4-1.75 times as high, when compared to initial level, low psoriatic erythrodermia treatment efficiency is considered to take place.
 Peroxide determination in biological fluids / 2293334
Invention relates to method for determination of peroxide group content in lipids containing in biological fluids. Claimed method includes sampling of tested fluid, extraction of organic components by sample treatment with 3-fold amount of chloroform as solvent. After extraction solvent is vaporized in inert gas flow , further total amount of lipids is determined by gravimetric analysis. Then sample is treated with glacial acetic acid up to total protein deposition in flake form. Then reagent mixture comprising KJ and CH3COOH in ratio of 1.0-1.5 l CH3COOH per 100 g KJ is introduced into tested sample to provide redox-interaction with globulated protein molecules up to total iodine reducing. Sample is placed in dark room for 1.5-2 h and further released iodine amount is determined by titrimetric method. And on the base of obtained data peroxide group content in lipids is determined.
 Method for predicting low intensity millimetric bandwidth radiation treatment effectiveness in treating patients for cardiovascular diseases / 2292557
Method involves determining potassium concentration in patient saliva before and after the first treatment applied to biologically active points of general systemic activity with electromagnetic radiation in wavelength bandwidth of 3.8-5.6 mm having specific noise power of 1.0-4.0 mcW/cm2. Potassium level dropping 1.3 times as low, therapy effectiveness is to be predicted. The potassium level reduction being less than 1.3 times as low or increase taking place, further treatment ineffectiveness is to be predicted.
Method for predicting respiratory distress syndrome in premature baby birth / 2291445
Method involves analyzing pregnant woman blood when threatened by premature birth and labor risk during 7 days. Nitrite and malonic dialdehyde concentrations are measured for determining miscarriage threat availability at early gestation period. Discriminant function is calculated from formula Y=8.87_0.1xX1-0.74xX2-3.05xX3, where X1 is nitrite concentration in mcmole/l, X2 is the and malonic dialdehyde concentration in nmole/l, X3 is the miscarriage threat availability at early gestation period: 2-presence, 1 - absence. Y being less than zero, respiratory distress syndrome is to be predicted.
Method for evaluating viability in unmature neonatals at respiratory distress-syndrome (rds) / 2290865
It is necessary to carry out biochemical blood assay, moreover, in peripheral blood one should detect the concentration of plasminogen, nitrites and evaluate respiratory failure by Silverman's scale during the first 2 h of life and calculate discriminant function (Y) by the following formula: Y=536+8.11xX1-17.79xX2-76.54xX3, where X1 - concentration of plasminogen, X2 - evaluation by Silverman's scale, X3 - concentration of nitrites and at Y>0 it is possible to evaluate neonatal's state as viable. The suggested method is very simple in usage.
Method for predicting titanium nickelide implantation consistency / 2286579
Method involves studying whole blood state by applying radiofluorescence analysis approach with synchrotronic radiation, determining quantities of microelements like Se, Zn, Cu and Mn in whole blood. Early and late implantation consistency is to be predicted, when detecting 20% selenium concentration fall, zinc concentration twice as low at the third day in early postimplantation period relative to reference values, and 40% zinc concentration fall, copper concentration increase 1.3 times as high and manganese concentration increase 1.5 times as high at the fourteenth day, and 40% zinc concentration fall at the sixtieth day, and manganese concentration increase twice as high at the one hundred twentieth day in late postimplantation period when having correspondence to the reference values. Intact animal group indices and microelements concentrations in other periods are taken as the reference values.
Method for predicting clinical course of bronchopulmonary diseases and pulmonary carcinoma / 2280868
Method involves examining expired air moisture condensate for determining sodium, potassium, calcium content and hydrogen index. Bronchopulmonary diseases remission stage is predicted depending on sodium, potassium, calcium concentration and hydrogen index, and pulmonary carcinoma diagnosis is excluded or confirmed.
Method for predicting the development of postgastroresectional peptic ulcers / 2304785
The present innovation deals with predicting the development of postgastroresectional peptic ulcers in patients due to detecting the level of gastrin after stimulation, pepsinogen, prostaglandins PGE2 and PG F2α, correspondingly, in mucosa of gastric stump and in mucosa of gastroenteroanastomosis. At increasing gastrin level up to 12.5 pM/l and higher, that of pepsinogen up to 10.0 mcg/l and higher and decreasing the level of prostaglandin PGE2 up to 570 and lower and at the decrease of prostaglandin PG F2α level up to 652 ng/g and lower one should predict the development of postgastroresectional peptic ulcer. Application of the present innovation enables to increase the accuracy in predicting the development of postgastroresectional peptic ulcers.
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FIELD: medicine, ophthalmology. SUBSTANCE: due to biochemical testing lacrimal fluid (LF0 it is necessary to detect the concentration of cholesterol, activity of superoxide dismutase (SOD) and catalase, in blood one should detect the concentration of nitrites and based upon the values obtained calculate Function 1 and Function 2. Function 1 = -3.4+0.29(NO2)+32.5(cholesterol)-0.97(catalase)-17.28(SOD) - corresponds to Y-axis values and Function 2 = -7.14+0.17(NO2)+37.2(cholesterol)+2.1(catalase)-17.28(SOD) - corresponds to X-axis values on the scheme to detect lesion type. If the point obtained is on the field of the 1st scheme one should detect the presence of nonproliferative DR form in a patient at no risk of proliferation; if it is on the field of the 2nd scheme - nonproliferative DR form with the risk of proliferation; if it is on the field of the 3d scheme - proliferative form of retinopathy in the phase of neovascularization. Application of the present method enables to carry out diagnostics in due time, differentiation in doubtful cases directed towards adequate treatment and decreasing the risk for DR progressing. EFFECT: higher accuracy of diagnostics. 3 ex
The invention relates to medicine, in particular to ophthalmology, and can be used in the diagnosis of stages of diabetic retinopathy. Known analog of the method of predicting the development of diabetic retinopathy (DR) is detected in the tear fluid (LF) antibodies to S-antigen in the retina. According to this method, the determination in SG level of antibodies with a titer of more than 1/128 shows the development of OTHERS (Zaitseva NS, Slepov O.S., Lee PS, Dudnikova L.K. TO the immunodiagnostics of diabetic retinopathy. Journal of ophthalmology. - 1990, No.1, P.46-49). This method allows us to predict only the probability of OTHERS and does not allow one to assume the risk of developing a particular stage. The closest to the technical essence and the achieved effect and selected as a prototype is the method of differential diagnosis of diabetic retinopathy in patients with non-transparent media of the eye when normoglycemia (EN 2020861, publ. 15.10.1994). However, in this prototype diagnosis stages, ETC based on only one biochemical indicator of the concentration of glucose in tear fluid, which is quite unstable, even during the days parameter. The task, which is aimed by the invention is the provision of diagnostics development stages, ETC. p> The technical result - timely diagnosis, differentiation in doubtful cases, aimed at adequate treatment and reducing the risk of progression, ETC.This problem is solved due to the fact that in the claimed method for the diagnosis of diabetic retinopathy by biochemical studies, including the study of the lacrimal fluid, characterized in that the tear fluid to determine the concentration of cholesterol, the activity of superoxide dismutase and catalase in the blood to determine the concentration of nitrite, based on the received indicators are calculated Function 1 and Function 2; Function1=-3,4+0,29(NO2)+32,5(cholesterol)-0,97(catalase)-17,28(SOD) corresponds to the value on the y axis and Function2=-7,14+0,17(NO2)+37,2(cholesterol)+2,1(catalase)-3,15 (SOD) corresponds to the value on the x-axis on the map to determine the type of lesion penetration obtained points 1 card on the field to determine whether the patient nonproliferative Dr form without the threat of proliferation; field2 - nonproliferative Dr form with the threat of proliferation; pole - proliferative retinopathy in the phase of neovascularity. The criteria statistically significant in our studies are: nitrite peripheral blood and cholesterol, catalase, SOD in LF. These indicators were determined in biochemical laboratory and by the following methods. Determination of nitrites in the peripheral blood. The determination was carried out according to the method Yemchenko D., Tsyganenko I., Kovalevskaya T.V. "a Universal method for the determination of nitrate in biological media of the organism". Clinical and laboratory diagnostics, 1994, No. 6, P.19-20. According to this method, nitrate is determined as nitrite after restoring their spongy cadmium. To determine the fraction of nitrite, the authors propose a similar algorithm research, but without passing through the cadmium column. In centrifuge tubes with a capacity of 10 ml was placed 1.0 ml of 50% zinc sulfate, and 1.0 ml of a 17% solution of ferricyanide of iron, 1.0 ml of the analyzed blood. Then add 2,0 ammonium chloride buffer (pH 9,6) and 5.0 ml of distilled water. Take an aliquot of centrifugate volume of 5 ml in a flask with a capacity of 50 ml, add 2.5 ml of ammonium chloride buffer, 2.5 ml of distilled water, mix. Then in the flask add 5 ml of 0.25% solution of streptocide, 1 ml HCl (1/2) and after 5 min - 1 ml of 0.1% solution of NED. The solution was adjusted to the mark with distilled water, mix and leave for 10 minutes after this time samples photometrate on fotoelektrokalorimetry KLF-3 at a wavelength of 540 nm (green filter in a cell with optical path length of 5 cm against a blank sample. The concentration of nitrite is determined by the calibration curve. the La build in a volumetric flask with a capacity of 50 ml contribute to 0.25, of 0.5, 1, 2.5 and 5 ml of a solution of potassium nitrate with nitrite ion, equal to 10 µg/ml, which corresponds to 0, of 0.05, 0.1, 0.2, 0.5 and 1 µg/ml, add 10 ml of distilled water and 5 ml of ammonium chloride buffer and elute through a cadmium column. The optical density was measured against a blank solution. The content of nitrites (X, mg/l) in the analyzed samples of biological media are calculated according to the formula X=C1×V1×V2/V3×V4, where C1the concentration of nitrate ion in the final reaction solution, was found in the calibration curve (mg/ml); V1- total protein-free extract (ml); V2- the total volume of the final reaction mixture (ml); V3- the volume of sample taken for analysis (ml); V4- the amount of protein-free extract, taken for further analysis. Enzymatic method for quantitative determination of total cholesterol in the tear fluid. Cholesterol and its esters are distinguished from lipoproteins by the action of detergents. Cholesterylester hydrolyzes esters of cholesterol produced cholesterol under cholesteroloxidaze oxidized. In the process of reaction is the indicator cinnimin from hydrogen peroxide and 4-aminophenazone in the presence of phenol and peroxidase. The cholesterol ester + H2On - cholesterylester→cholester the n+fatty acid. Cholesterol + O2- cholesterol oxidase (CHOD)→cholesten-3 he+N2About2 2 H2O2+4-aminophenazone+phenol - peroxidase→quinonimine + 2 H2About
Mix. Incubate for 5 minutes at a temperature of 37°and 10 minutes at 15-25°C. Measure the optical density of the samples and standard relative form with respect to the substrate when the length of 540 nm on the AF analyzer M (Finland). The intensity of the resulting color is directly proportional to the concentration of cholesterol. Determination of catalase. The principle of the method is based on the ability of hydrogen peroxide to form salts of molybdenum stable coloured complex. For determination of catalase activity to 1 ml of 0.03% hydrogen peroxide solution was added to 0.02 ml of tears. In idle test instead of tears made of 0.02 ml of distilled water. The reaction was stopped after 10 minutes by addition of 0.5 ml of 4% solution of ammonium molybdate. Intensity developed the paint is measured spectrophotometrically at a wavelength of 410 nm against blank samples. Catalase activity was calculated by the formula A=(Ehal-Tu)×V×t×K; where a is the catalase activity in µkat/ml, Ehol and Tu - extile idle and experimental samples, V is the volume of the applied sample t - time intubation (600 sec), K - coefficient millimolar extile hydrogen peroxide 22,2×10 ákat/ml Determining the concentration of superoxide dismutase. The determination was carried out according to the method Averagerank. "Comprehensive determination of the activity of superoxide dismutase and glutathione reductase in erythrocytes in patients with chronic liver disease", Laboratory work, No. 11, 1988, p.48-49. For the determination of SOD activity 20 ml tears were injected in 1 ml incubate environment containing 0,41 mm narasinga of tetrazole, 0.33 mm EDTA, 0.01 mm N-methylpentane methyl sulfate. Measured optical density in a spectrophotometer SF-46 at a wavelength of 540 nm, then added to microcuvette spectrophotometer 0.1 ml of 0.8 mm NADH, mixed and left in the dark for 10 minutes then re-measured optical density. About the reaction was judged according to the difference between the first and second indicators spectrophotometer. The enzyme activity was expressed in arbitrary units. The study included 60 patients, all of them had a second type of diabetes, we identified 12 nonproliferative stage, ETC without the threat of proliferation at 24 nonproliferative stage OTHERS with the threat of PR is literaly and 24 proliferative stage of retinopathy in phase neovascularization. Used classification Vratsata, 1997. Patient age ranged from 44 to 71 years, 15 men and 45 women. On the basis of a discriminant analysis was built map and determined the functional dependence of the influence factors on the prediction of the development of OTHERS. To use this card, you need to Function formula 1 to calculate the value based on available data for biochemical research, and mark this value on the x-axis. Then similar calculations are made for Function 2, the resulting value is marked on the ordinate axis. Hit points gained through the intersection of these values, with one of the three fields of the card and determines the presence in a patient of this type of lesion with probability 96,7%. 1. nonproliferative Dr form without the threat of proliferation. 2. nonproliferative Dr form the threat of proliferation, 3. proliferative form of retinopathy in phase neovascularization. Function 1=-3,4+0,29 (NO2blood)+32,5(cholesterol tear fluid) to 0.97 (catalase) - 17,28 (SOD). Function 2=-7,14+0,17 (NO2blood)+37,2(cholesterol tear fluid)+2,1 (catalase) - 3,15 (SOD). The accuracy of the model 96,7%. Examples Example 1. Ignatova Nina, 66 years old, has diabetes type 2 diabetes for 8 years, takes maninil. Related diseases - hypertension 2 St, 2 St, 4 the risk. Diabetic Reti is Opatija identified on the periodic health examination in 2001. Objectively the fundus of the eye OU: optic nerve disc pale pink, clear boundaries. The veins are dilated, tortuous, the caliber of their uniform. Artery partially sclerotic (fibrous). In the retina isolated microaneurysms, petechial hemorrhages. Laboratory: nitrite peripheral blood =26 mg/l, Cholesterol tear fluid =0.035 mmol/l, Catalase =2,9 MCAT/ml and SOD=0,42 $ /ml in LF. Function 1=-3,4+0,29(26)+32,5(0,035)-0,97(2,9)-17,28(0,42)=-4,78 Function 2=-7,14+0,17(26)+37,2(0,035)+2,1(2,9)-3,15(0,42)=4,18 This means that this map is located in the first sector that defines it as OTHERS nonproliferative form without the threat of proliferation. This conclusion is consistent with data from clinical examination and fluorescein angiography. Example 2. Gerasimenko Zinaida Andreevna, 66 years old, has diabetes type 2 diabetes for 11 years, takes diabecon. Related diseases - hypertension 2 St, 2 St, 4 the risk. Diabetic retinopathy is detected in 2000, the coagulation-type "grid". Objectively the fundus of the eye OU: pale optic nerve disc, border clear. The veins are dilated, tortuous, the caliber of their uniform. Artery completely sclerotic (fibrous), Salus III. In the retina of a single firm, soft exudates, preretinal hemorrhage. In the macular region dyspigmentation, periferiche foci of chorioidea after Luke. Laboratory: nitrite peripheral blood =35 mg/l</> Cholesterol tear fluid =0.09 mmol/l, Catalase =3,2 MCAT/ml and SOD=0,32 $ /ml in LF. Function 1=-3,4+0,29(35)+32,5(0,09)-0,97(3,2)-17,28(0,32)=1 Function 2=-7,14+0,17(35)+37,2(0,09)+2,1(3,2)-3,15(0,32)=7,8 This means that this map is located in the second sector, which defines it as OTHERS nonproliferative forms with the threat of proliferation. This conclusion is consistent with data from clinical examination and fluorescent angiography. Example 3. Bazhanov Lyudmila Ivanovna, 54 years old, has diabetes type 2 diabetes for 16 years, taking insulin within the last 3 years. Related diseases - hypertension 2 St, 2 St, 4 the risk., chronic pyelonephritis. Diabetic retinopathy is detected in 2001. Objectively the fundus of the eye OU: optic nerve disc pale pink, clear boundaries. The veins are dilated, tortuous, the caliber of their uniform. Artery partially sclerotic (fibrous). In the retina of multiple solid, single soft exudates, petechial hemorrhages. On the right eye pipelare, on the left peripapillary and in the retina are defined neovasculature. Laboratory: nitrite peripheral blood =40 mg/l, Cholesterol tear fluid =0,069 mmol/l, Catalase =2 mkat/ml and SOD=0,24 $ /ml in LF. Function 1=-3,4+0,29 (40)+32,5(0,069)-0,97(2)-17,28(0,24)=4,35 Function 2=-7,14+0,17(40)+37,2(0,069)+2,1(2)-3,15(0,24)=5,67 This means that this map is located in the third the sector, what defines it as OTHERS proliferative forms. This conclusion is consistent with data from clinical examination and fluorescein angiography. The way to diagnose forms of diabetic retinopathy (DR) by biochemical studies, including the study of the lacrimal fluid (LF), characterized in that the tear fluid to determine the concentration of cholesterol, the activity of superoxide dismutase (SOD) and catalase in the blood to determine the concentration of nitrite, based on the received indicators are calculated Function 1 and Function 2, Function 1=-3,4+0,29(NO2)+32,5 (cholesterol) to 0.97(catalase) - 17,28(SOD) corresponds to the value on the y axis and Function 2=-7,14+0,17(NO2)+37,2(cholesterol)+2,1(catalase) - 3,15(SOD) corresponds to the value on the x-axis on the map to determine the type of lesion penetration obtained points 1 card on the field to determine whether the patient nonproliferative Dr form without the threat of proliferation; on the 2 - nonproliferative Dr form with the threat of proliferation; field 3 - proliferative retinopathy in phase neovascularization.
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