The enzyme preparation having endogenously activity decomposition of rhamnogalacturonan-ii (rg-ii), the method of its production, the strains of the fungus penicillium daleae and penicillium simplicissimum with endogenously activity decomposition of rhamnogalacturonan-ii(rg-ii) (options), the method of selection of strains with endogenously activity decomposition of rhamnogalacturonan-ii(rg-ii) and the use of enzyme preparation |
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IPC classes for russian patent The enzyme preparation having endogenously activity decomposition of rhamnogalacturonan-ii (rg-ii), the method of its production, the strains of the fungus penicillium daleae and penicillium simplicissimum with endogenously activity decomposition of rhamnogalacturonan-ii(rg-ii) (options), the method of selection of strains with endogenously activity decomposition of rhamnogalacturonan-ii(rg-ii) and the use of enzyme preparation (RU 2220203):
C12S3/12 - Treatment of pectin or starch
C12H1/15 - with enzymes
                                                                 
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The invention relates to biotechnology and concerns enzyme preparation decomposition of rhamnogalacturonan II (RG-II) with an activity of endo- -L-rhamnopyranose -(1-->3')-D - apiformis-hydrolases and endo- -L-fucopyranoside -(1-->4)-L-rhamnopyranose-hydrolases derived from a strain of Penicillium daleae CNCN 1-1578 (LAV 2) and strain Penicillium simplicis-simum CNCN 1-1577 (IPVI). The enzyme preparation is get through the cultivation of a strain of Penicillium 1-1577 or 1-1578 deposited in STSM, separating it from the supernatant culture fluid or supernatant crushed strains. The method of selection of strains with the indicated activity provides for the selection of strains germinated in culture medium containing Monomeric RG-II. The obtained enzyme preparation is used as a tool for destruction and modification of RG-II, as a means to improve filterability, or facilitate the preparation of concentrated fruit juice, or to improve clarification as a means for cleaning the filter substrate and means for maceration or hydrolysis of plant tissues. 9. C. and 1 C.p. f-crystals, 20 ill., 7 table.
Description text in facsimile form (see g is gidrolizny activity decomposition of rhamnogalacturonan-II (RG-II), characterized in that it has endo--L-rhamnopyranose-(1 3’)-D-apiofuranoside activity and endo--L-fucopyranoside-(14)-L-rhamnopyranoside activity and derived from a strain of the fungus Penicillium genus selected from a strain of Penicillium daleae CNCM 1-1578 (LAV 2) and strain Penicillium simplicissimum CNCM 1-1577 (IPV 1).
2. The strain of the fungus Penicillium daleae CNCM 1-1578 (LAV 2) with endogenously activity decomposition of rhamnogalacturonan-II (RG-II).
3. The strain of the fungus Penicilliuim simplicissimum CNCM 1-1577 (IPV-1), with endogenously activity decomposition of rhamnogalacturonan-II (RG-II).
4. The method of obtaining the enzyme preparation under item 1, comprising culturing the strain of the fungus Penicillium genus selected from a strain of Penicillium daleae CNCM 1-1578 (LAV 2) and strain Penicillium simplicissimum CNCM 1-1577 (IPV 1), in culture medium suitable for production of the enzyme, present and degrades RG-II these strains, highlighting the enzyme preparation from the supernatant culture fluid or from the supernatant of these crushed strains.
5. The method according to p. 4, characterized in that after culturing the separated mycelium, crushed it, separating insoluble material and produce the supernatant, sostojanija of rhamnogalacturonan-II (RG-II), wherein the selected samples containing fungi of the Penicillium genus, cultivated in the above-mentioned samples of fungi in culture medium containing Monomeric RG-II, and separate the mushrooms that grow on culture medium containing RG-II, and with the ability to decomposition of RG-II.
7. The enzyme preparation under item 1 as a means for the destruction or modification of RG-II.
8. The enzyme preparation under item 1 as a means for improving the filterability or facilitate the preparation of fruit juice concentrate or to improve clarification.
9. The enzyme preparation under item 1 as a means for cleaning the filter substrates used for filtering fruit juices, vegetables and their derivatives.
10. The enzyme preparation under item 1 as a means of maceration, liquefaction, or complete or partial hydrolysis of plant tissues.
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