The microbiological method of processing cellulose-containing materials

 

(57) Abstract:

The invention relates to biotechnology and can be used in the processing of cellulose-containing raw material with the aim of obtaining feed for farm animals. On cellulose-containing material is grown consortium of microorganisms containing a culture of the microorganism Syncephalastrum racemosum (Mucoralus) and culture of the microorganism Allescherie terrestris, taken in the ratio of from 1:10 to 10:1. The way microbiological processing reduces the time of decomposition of cellulose-containing materials. 6 C.p. f-crystals.

The invention relates to the field of biotechnological production and can be used in the processing of cellulose-containing raw material with the aim of obtaining feed for livestock or organo-mineral fertilizers, and also upon receipt of the technical ethyl alcohol, paper, cardboard, synthetic fibres.

Annual natural increase of the pulp in the world is about 100 billion tons. Even the use of part of the cellulose-containing raw material leads to the accumulation of huge amounts of cellulosic waste: straw, sawdust, bark, substandard wood, grain waste, and so on, practically the coy-cost pulp. The complexity of the pulp due to the duration of degradation of the cellulose molecules.

It is known that straw, sawdust, consisting of about 70% cellulose and 10 to 20% of the lignin must be destroyed cellulolytic microorganisms to simple sugars. Received simple sugars can be used later as an energy source, for example, nitrogen-fixing microorganisms present in the soil and are able to fix atmospheric nitrogen available for plants.

The digestion of cellulose in nature due to soil microorganisms is very long and takes place within a few months even under favorable external conditions. In particular, (see Antunes, L. A. Agr. Siol. Technol. 1986. V. 29, N. 3, pp. 533-543), it is known that in natural conditions on cellulose-containing materials natural Association of cellulolytic microorganisms is formed only after 120 days.

The known method of enzymatic processing with the use of culture micromycete Paecilomyces variotii cellulose-containing materials (RU, patent 2094414 C 05 F 11/08, 1997) with the aim of obtaining organic fertilizers. The process takes up to 25 days.

The known method of enzyme , 997) used to feed animals. The process lasts up to 10 days.

A method of obtaining biologically active feed from cellulose-containing materials (RU, patent 2097979 A 23 K 1/00) using a culture of the fungus Agricus lisporus. The process lasts up to 7 days.

The technical problem to be solved by the present invention is to provide opportunities for accelerated microbiological processing of cellulose-containing materials.

The technical result is obtained as a result of implementation of the invention is to reduce the time of decomposition of cellulose-containing materials while reducing the number of initial lignin and polysaccharides in food processing.

To achieve the technical result of the proposed use to the treatment of cellulosic waste Association of microorganisms containing a culture of the microorganism Syncephalastrum racemosum (Mucoralus) and culture of the microorganism Allescherie terrestris, taken in the ratio of from 1:10 to 10:1. Mostly use the strain of microorganism Syncephalastrum racemosum (Mucoralus), collection number GSB TBG from the Collection of cultures of microorganisms of the State scientific research Institute of protein biosynthesis we State scientific-research Institute of biosynthesis of protein crops. However, there may be used and other strains of these cultures. Preferably, the pre-cellulose-containing material is moistened to a moisture content of 60-80%. Mainly, the content of dry cells used consortium is from 1 to 6% of the content of lignin in the original cellulosic feedstock. Usually processing a cellulose-containing material is carried out within 1-5 days. Mostly, processing is subjected to the source material with the content of cellulose and hemicellulose from 70 to 88% of absolutely dry matter and lignin from 5 to 25% of absolutely dry matter. It is desirable to use shredded cellulose material.

Mainly used strains of the microorganisms listed above, have the following characteristics:

1. The strain of microorganism Syncephalastrum racemosum (Mucoralus), collection number GSB TBG from the Collection of cultures of microorganisms of the State scientific research Institute of biosynthesis of proteins extracted from soil by accumulating crops, followed by selection.

The strain has the following morphological features: low Mineralny the fungus hyphae deceptively, aerobe, and the firm soil forms belanica carbon uses sugar, pectin, starch, cellulose, organic acids. The strain is able to produce single-cell proteins, as well as cellulolyticus enzymes. The growth rate of strain is approximately 0.1 h-1.

2. The strain of microorganism Allescherie terrestris, collection number GSB TBG in the Collection of cultures of microorganisms of the State scientific research Institute of protein biosynthesis cultures isolated from straw of cereal crops through breeding.

The strain has the following morphological features: Mineralny mushroom, mycelium depart branched ends, which develop bundles of conidial chains of conidia angular, with the disintegrating chains they sometimes remain United corner, the mycelium colorless, ground penetrating a substrate as a carbon source strain uses glucose, galactose, sucrose, raffinose, maltose, cellulose and lignin. Strain reproduces with spores. The strain is able to synthesize single-cell proteins, cellulolyticus enzymes. The growth rate of strain is the average of 0.1 h-1.

The invention can be illustrated by the following examples of implementation.

1. To prepare inoculum cellulose substat with a cotton swab and sterilize the resulting substrate at 1.5 ATM for about 30 minutes In sterilized and cooled medium is seeded with a suspension of these strains of microorganisms, pre-grown on suslovo agar at a temperature of approximately 28oC. the Density of planting is 0.01 gram of dry cells of microorganisms per gram of substrate. Seeded suspension is cultivated in an incubator for 10-15 days at a temperature of approximately 28oC. Obtained in a similar manner seeds material used for inoculation of cellulose-containing medium in the fermenter.

2. In the chamber volume of 3000 ml asleep shredded wood bark and peat, taken in the ratio 3:7. The mixture was moistened with water to a moisture content of 70% and contributed seed material, in which the ratio of strains of microorganisms is 1: 9, obtained previously, in the amount of 3% of the lignin content in the mixture. The cultivation was carried out for three days while maintaining a constant humidity. In destructiona cellulose-containing material in polysaccharides 40% of dry matter and lignin - 8%. In the original substrate of polysaccharides was 85%, while the lignin content of 12%. In the fermentation process collapsed 53% polysaccharides and 34% of lignin.

3. In the camera of 3 DM<'s the logs 75%. Sowed seeds material obtained in example 1, but with a ratio of strains of these microorganisms as 9:1. The total number of seeds material amounted to 4% of the content of lignin. Fermentation was carried out for two days while maintaining a constant humidity. In destructiona raw content of cellulose and hemicellulose was 53% of the dry weight, and the content of lignin - 3%. As in the original mixture (straw and peat) contained polysaccharides 80%, and lignin - 8%, as a result of processing has destroyed 32% of Polisario and 53% of lignin.

4. In the camera of 3 DM3put chopped straw and moisten it with water to a moisture content of 65%. Added the seed material is obtained analogously to example 1 at a ratio of strains of microorganisms as 1:1. The total number of seeds material was approximately 6% of the content of lignin in raw materials. Fermentation was carried out during four days while maintaining a constant humidity. After processing, the content of polysaccharides was 35%, and lignin - 5%. Because the feedstock contained polysaccharides 75%, and lignin 15%, as a result of processing collapsed 54% polysaccharides and 70% of the lignin.

5. In the camera of 3 DM3moved the wheelie seed material, is obtained analogously to example 1, with a ratio of strains of microorganisms 2:1. The total amount of inoculum was approximately 5% of the content of lignin in the feedstock. Fermentation was carried out for 2 hours while maintaining a constant humidity. After processing, the content of polysaccharides was 46%, and lignin - 14%. In the raw material they contained, respectively, 70 and 23%. As a result of processing collapsed 36% polysaccharides and 27% lignin.

6. In the camera of 3 DM3put sawdust from wood, having humidified with water to a moisture content of 65%. Added the seed material is obtained analogously to example 1, with a ratio of strains of microorganisms such as 1.5:1. The total number of seed amounted to 6% of the content of lignin in the feedstock. Fermentation was carried out for 3 hours while maintaining the specified humidity. After microbiological processing of polysaccharides was 37% when the lignin content of 13%. In the source material they contained 72 and 21%, respectively. As a result of processing collapsed 49% polysaccharides and 36% of lignin.

The use of the obtained semi-product in the future, perhaps when receiving target products: feed weste is also treated by the above method, a cellulose-containing material is reduced to 2-4 days.

1. The microbiological method of processing cellulose-containing materials, including cultivation on cellulose material of microorganisms, characterized in that the cellulose material is grown consortium of microorganisms containing a culture of the microorganism Syncephalastrum racemosum (Mucoralus) and culture of the microorganism Allescherie terrestris, taken in the ratio of from 1:10 to 10:1.

2. The method according to p. 1, characterized in that use strain of microorganism Syncephalastrum racemosum (Mucoralus), collection number GSB TBG in the Collection of cultures of microorganisms of the State scientific research Institute of biosynthesis of proteins.

3. The method according to p. 1, characterized in that use strain of microorganism Allescherie terrestris, collection number GSB TBG in the Collection of cultures of microorganisms of the State scientific research Institute of biosynthesis of protein crops.

4. The method according to p. 1, wherein the pre-moistened cellulose-containing material to a moisture content of 60-80%.

5. The method according to p. 1, characterized in that the content of dry cells used consortium is from 1 to 6% of the content of lignin in the original cellulose-containing raw material.

6. Spouses fact, the content of cellulose and hemicellulose in the feedstock is from 70 to 88%, and the content of lignin from 5 to 25% of the absolute dry substance.

 

Same patents:

The invention relates to biotechnology, Microbiology, remediation of contaminated land

The invention relates to Phytopathology, in particular to a method of determining fungal pathogens using specific primers in the polymerization reaction
The invention relates to biotechnology and medicine
The invention relates to biotechnology and can be applied in the leather industry and veterinary
The invention relates to biotechnology and can be used for the prevention and treatment of cancer

The invention relates to agriculture

The invention relates to the microbiological industry, and specifically to a method for producing salts of gluconic acid (gluconate) and citric acid

The invention relates to biological processing of plant products and the bioconversion of agricultural waste products of animals, as well as to the livelihood of man
The invention relates to agriculture, in particular fertilizers and method of their production

The invention relates to agriculture and can be used in the processing of organic waste

The invention relates to agriculture and can be used to obtain new bacterial fertilizers
The invention relates to biotechnology and agriculture, namely, production of organic fertilizers
The invention relates to a biological means of increasing crop plants and protect them from diseases
The invention relates to agriculture and can be used in the production of fertilisers from waste and poultry waste ethyl alcohol
The invention relates to agriculture and can be used in the recycling of livestock farms and poultry farms and remediation of contaminated oil and petroleum products land

The invention relates to agriculture, in particular the production of biological fertilizers
Up!