The method of obtaining food biosorbent

 

The method of obtaining food biosorbent by microbiological synthesis based on receipt of the deep culture of the edible mushroom Pleurotus ostreatus. The cultivation is carried out with use of strains VKPM F-697 and F-720 for 48 h under conditions of aeration on starch-containing liquid nutrient medium in the absence of additional nitrogen sources. The method is environmentally friendly and cheap, allows you to get the chitinous biosorbent with high sorption capacity on the basis of microbiological synthesis without the use of chemical processing. Obtained in this way biosorbent has a stimulating effect on the process of beer fermentation, which is expressed in the increase of alcohol on the main stage of fermentation. 6 table.

The invention relates to the microbiological industry, and specifically to methods for biosorbent by microbiological synthesis, which can be used as activator beer fermentation.

A method of obtaining chitin-containing biosorbents based on the use of lower fungi Allomyces, Aspergillus Renicillium, Misog, Phycomyces, Choanophora and other (1), the biomass of which directly can not be used for food.

The lack of takaraivossa citric acid, antibiotics), which greatly complicates the process of obtaining ecologically clean product. In addition, the proposed allocation method biosorbent associated with the use of acids and alkalis, which on the one hand defines this method as environmentally harmful, and on the other, significantly increases the cost of implementation of this method in production.

A method of obtaining chitin-containing biosorbent from fruit bodies of edible mushroom Pleurotus ostreatus (2).

This method, as described above, is environmentally harmful, because it is based on the use as raw material dry fruit bodies, which, as a rule, have a certain natural background radio and toxinotype. The use of such materials in technological production creates additional difficulties in the purification process of the mycelium of natural pollution.

This method is also based on the use of acids and alkalis, which increases the final product.

The closest technological essence and the achieved result is a method of obtaining chitin-containing biosorbent based on the use of fruit bodies and deep culture of the higher fungi (3).

This method includes processing g is TBE mushroom mycelial mass use mycelial mass of basidiomycetes, obtained after the alkali treatment and the washed product is a fibrous mass before drying sequentially process miscible with water and organic solvents in decreasing order of their boiling points, and after drying impregnated with solutions of salts of metals of ferromagnets, with subsequent translation of these insoluble salts in the ferromagnetic state by restoring to metal or obtain ferromagnetic compounds.

However, the disadvantage of this method is that it is based on the use of a range of organic solvents, which not only defines this method as environmentally harmful, but also considerably increases the cost of its implementation in production.

The objective of the invention is to develop environmentally friendly and cheap to manufacture method of obtaining chitin-containing biosorbent with high sorption capacity on the basis of microbiological synthesis without the use of chemical processing. This is achieved by the described method of obtaining fungal biomass, including deep cultivation of the fungus Pleurotus ostreatus in the conditions of aeration in a nutrient medium detachable-topping-up method followed by the separation of biomass and distinctive 6,0-7,5 in the absence of additional sources of nitrogen, such as peptone and others, the content of the flour in a nutrient medium does not exceed 1%.

Used in the way that strains-producers of Pleurotus ostreatus VKPM F-697 and F-720 deposited in Russian national collection of industrial microorganisms and stored in the private collection of the author. In General, the proposed method allows to obtain not only environmentally friendly biosorbent, but also significantly reduces the process of obtaining chitin-containing complex with a high sorption capacity. Beneficial to increase chitinous biosorbent reduction in nutrient nitrogen source.

Example 1.

Culture grown on agar medium. Then transfer it to the flask at 150-200 ml with medium consisting of Nikolenko wort (4ofor Billing), without petone pH 6,0-7,5. Growing culture is performed on a rocking chair at a temperature of 26-28oWith in 48-72 hours. The resulting culture "zakolerovat" within 4-8 hours and used as seed material for the next stage of the fermentation. Biomass yield at this stage 20-25 g/l nutrient medium.

For fermentation using 15 l or 100 l or 300-liter fermenters filled with a nutrient medium with the addition of 0.1 to 1.0 % surfactant by volume.

Processto seeding culture is poured under sterile conditions in the amount of 40-50% by volume of the liquid. Topping sterile nutrient medium is carried out directly after weaning the culture fluid. When this surfactant is not added. Cultivation detachable-topping-up method are within 1-3 months, after which all the culture fluid is drained and the process begins with the use as a seed surface agar culture. At the end of each fermentation biomass is filtered off, washed and dried at a temperature of 50-60oC.

The yield of dry biomass at the stage of fermentation is 10-15 g/l of nutrient medium. The proposed method can be played on various media used for the strain of Pleurotus ostreatus F-697 and F-720.

Below is data on the chemical composition of biomass under cultivation of strain F-697 wheat-starch flour (flour - 1%, starch and 1.5%) (see table 1).

Elemental analysis (%): N total - 5,15; N protein - 3,14; - 59,5; N? 7.04 baby mortality; protein - 21,1; ash - 4,4; lipids - 2,1; moisture - 6,5; Hemingway - 63,5.

Example 2.

The process of cultivation of the strains are as described in example 1, but with different content of nitrogen in the nutrient medium.

Thus obtained in this way biomass contents heringlake is not less than 60%, which is the spine of the obtained biosorbent presented in table 2.

The data presented in tables 2 and 3 show that the obtained biosorbent (submerged mycelium) has a high sorption capacity with respect to such toxic metals as cadmium, lead and zinc, and in the case of radionuclides such as cesium and strontium.

In addition, investigated the sorption activity of this product in relation to certain substances (table 4).

The influence obtained biosorbent as activator beer fermentation stage main stage on the example of "grassroots" fermentation.

As criteria to assess the influence of biosorbent on the fermentation process were alcohol yield and time required for completion of the main stage of fermentation.

In the quality control process used "bottom-fermented" hopped wort (12othe Balling), which was carried out brewer's yeast (the yeast Sacharomyces cerevisiae race A-14) without any additives.

The results of these experiments are presented in table 5.

The results presented in table 5, show that the obtained biosorbent stimulates the process of "grassroots" fermentation. Both types of dry product stimulates the release of alcohol. In addition, the wet product has bol table 6.

Literature 1. Muzarelli Q. A. A. Chitosan-glucan complex. Pat Cer. 2923302, 1979.

2. The'yash C. F., Masooma C. A., Tumanov, A. N., Demarin C. T., N. Kralin.N., The fungus P. T. a Study of the process of sorption of heavy metals by chitin of mushrooms Oyster. New perspectives in the study of chitin and chitosan. - Moscow, 1999, page 280, VNIRO.

3. Gorovoy D. F., Kosakov Century. And. Method of obtaining of chitinous material. Patent of the Russian Federation, 2073015, 1997

Claims

The method of obtaining food biosorbent by submerged cultivation of wood-degrading basidiomycete, characterized in that the use of strains of Pleurotus ostreatus BCP-F-697 and F-720, cultivation of conduct for 48 h under conditions of aeration at pH 6.0 and 7.5, followed by the separation of the biomass, and the cultivation lead to starch-containing environments in the absence of additional nitrogen sources.

 

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