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Method of obtaining functionalised derivatives of hyaluronic acid and formation of their hydrogels Invention relates to chemistry of polysaccharides. A method of obtaining functionalised derivatives of hyaluronic acid includes activation of at least one hydroxyl group of hyaluronic acid. Hyaluronic acid is taken in the form of a salt soluble in organic solvents. Activation is performed by interaction of the said salt of hyaluronic acid in a polar aprotic solvent with a carbonylating agent. The carbonylating agent is selected from phenyl ethers of carbonic acids and phenyl ethers of haloformic acid. After that, the obtained activated salt of hyaluronic acid by a reaction of a nucleophilic substitution is subjected to interaction with a compound of the general formula NH2-R. R is selected from NH2, an aminoalkyl group, alkyl or arylalkyl chains, a polyacryl chain, a polyoxyethylene chain of a drug agent, a polymer or a protein. Also described are functionalised derivatives of hyaluronic acid and based on them hydrogels. |
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Method of producing sulphated arabinogalactan derivatives Method involves reacting arabinogalactan with a sulphating complex while continuously stirring and heating. The sulphating complex used is a sulphamic acid-urea complex. Said complex is obtained by mixing equimolar amounts of sulphamic acid and urea in dioxane. Sulphation of arabinogalactan is carried out at temperature of 80-95°C and hydromodulus of 1:10 for 2.5-3.5 hours. The ratio of arabinogalactan to the sulphating complex is 1:13-25 (g:mmol). The product is separated by neutralisation and precipitation in ethyl alcohol. |
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Method of obtaining water-insoluble sulphur-containing chitosan-based biomaterial Invention relates to the field of organic synthesis. A method of obtaining a water-insoluble sulphur-containing chitosan-based biopolymer includes interaction of chitosan with a thiomethylating agent, preliminarily obtained by saturation of a formaldehyde solution with gaseous H2S, with molar ratio chitosan: formaldehyde: hydrogen sulphide 1:6:4, at a temperature of 60°C for 20-25 hours. |
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Group of inventions relates to biotechnology. Claimed are versions of method of obtaining solution, which contains purified capsular polysaccharides, from lysate of cells of selected Streptococcus pneumoniae serotype. Lysis of cells of selected S. pneumoniae serotype is carried out. Obtained lysate is clarified by centrifugation or filtration. After that, ultrafiltration and diafiltration of clarified lysate are carried out in salt-free water with obtaining retentate. Retentate pH is reduced to the value lower than 4.5. Acidified retentate solution is kept to sediment precipitate. After that, retentate solution is filtered or centrifuged to obtain clarified solution of capsular polysaccharide. Then, clarified polysaccharide solution is filtered through filter with activated carbon. Obtained filtered solution id ultrafiltered and diafiltered. Obtained concentrated purified solution of capsular polysaccharide is filtered through sterile filter. S. pneumoniae serotypes are selected from group, consisting of serotypes 1, 4, 5, 6A, 6B, 7F, 9V, 14, 18C, 19A, 19F and 23F. Also claimed is modification of claimed method for serotype 19A. Said modification includes carrying out ultrafiltration and diafiltration before acidification at 4°C at pH 6 in sodium-phosphate buffer, keeping acidified retentate solution at for at least 2 hours 4°C and bringing pH of clarified polysaccharide solution to value 6 before the stage of filtration with active carbon. Also claimed are solutions, containing purified capsular polysaccharides of selected S. pneumoniae serotype, obtained by claimed methods, and their application in production of pneumococcal vaccine. Also claimed are purified capsular polysaccharides of S. pneumoniae serotype 6A with molecular weight 640000-670000 Da and 19A with molecular weight 488000-525000 Da, separated from said solutions. |
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Method of producing biologically active pectin Invention relates to production of polysaccharides from plant material. The method involves extracting the material with a threefold amount of 1% ammonium oxalate solution for 1.5 hours on a boiling water bath three times. The material used is oil cake obtained after separating flavonoids from common tansy flowers. The obtained extracts are mixed, filtered through several layers of gauze and pectins are precipitated in the filtrate with threefold excess 96% ethyl alcohol. The obtained precipitate is then washed successively with 96% ethyl alcohol, acetone and ether and dried over concentrated sulphurica acid. |
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Method of obtaining low-molecular heparin Method of obtaining low-molecular heparin includes obtaining benzethonium salt of non-fractionated heparin in 0.05-0.5 M water solution of sodium chloride at temperature 50-60°C, pH=8.2-8.8 and weight ratio heparin/benzethonium chloride 1/(2,35-2.70), benzylation of heparin benzethonium salt, being carried out for 2-3 hours in medium of bipolar aprotic solvent with benzylchloride in ratio heparin/benzylchloride 1/(0.2-1.0), which is preliminarily subjected to activation in aprotic solvent for 15-20 min, precipitation of heparin benzyl ether by method of Spiro with ethyl alcohol, preliminarily saturated with water-free sodium acetate, with the following removal of protection from sulfo groups, carrying out β-elimination of benzyl ether with degree of heparin etherification 9-13% 1±0.5 N with alkali NaOH at temperature 55±5°C, duration of process is 40-60 min and weight ratio of reagents benzyl ether/alkali 1/(0.5-2). |
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Group of inventions relates to biotechnology and medicine. Disclosed is a polysaccharide which is isolated from the Bifidobacterium infantis NCIMB 41003 strain and has the structure [-β(1,3)-D-GalpNAc-β(1,4)-D-Glcp-]n, where said disaccharide unit repeats n times, which yields a polysaccharide with molecular weight greater than 100000 Da. The polysaccharide exhibits immunomodulating activity and is used in preparing medicinal agents for treating or preventing undesirable inflammatory activity, undesirable gastrointestinal inflammatory activity, rheumatoid arthritis and autoimmune disorders. |
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Modified polysaccharide has a general formula of the type (I). The modified polysaccharide is obtained by reacting primary polysaccharide with functional groups. A zwitterion compound is formed as a result. |
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Disclosed is a polysaccharide containing carboxyl functional groups, one of which is substituted with a hydrophobic alcohol derivative. Also disclosed is a pharmaceutical composition containing one of the disclosed polysaccharides and one active ingredient. |
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Complex of pectin biopolymer and acetylsalisylic acid Invention refers to biopolymers that can find application in chemical-pharmaceutical industry, medicine and veterinary science. The complex of pectin biopolymer and acetylsalicylic acid of formula wherein R=H, Me; n=98; m=2-11, prepared by a reaction of aqueous solutions of pectin and acetylsalicylic acid under mechanic-acoustic action with weight ratio of pectin: acid=1:(0.02-0.25) and ethanol precipitation of the prepared complex. |
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Method for complex processing of fish raw material for obtaining hyaluronic acid and collagen Skins of pond fish are flushed with cold flushing water during 10-15 minutes. They are crushed to the size of 2-3 mm. Water extraction is performed at the temperature of 40-45°C during 40-50 minutes at the ratio of crushed skins to water, which is equal to 1:1 at periodic mixing. Then, they are filtered; liquid fraction is dried in a spraying drier at the drier outlet product temperature of 60-65°C during 15-25 minutes so that hyaluronic acid is obtained. Solid fraction is subject to bleaching during 12 hours with hydrogen peroxide-salt solution that is prepared by mixing of 1 l of 3% hydrogen peroxide and 20 g of sodium chloride. Treatment of bleached solid fraction is performed with 1.0-1.2% solution of sodium hydroxide during 24 hours at the temperature of 20-25°C with further neutralisation of the obtained mixture with 3% boric acid solution. Treatment of swollen solid fraction is performed with Pancreatin ferment preparation solution taken in the quantity of 0.5-0.6% to the weight of solid fraction during 1.5-2.0 hours at the temperature of 37-40°C. Flushing of solid fraction is performed with cold flushing water for removal of Pancreatin residues so that collagen is obtained. The obtained collagen, depending on the purpose, is supplied for drying in drying chambers with forced air circulation at the temperature of 18-20°C during 12 hours and storage in dry ventilated rooms at the temperature of not higher than 20°C during 24 months or frozen to the temperature of minus 18 - minus 20°C and stored at the temperature of minus 18 - minus 20°C during 24 months. The liquid fraction dried in the spraying chamber is stored at the temperature of 0-4°C during 12 months or dissolved in physiological buffer solution. |
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Method for sterilisation by filtration of diluted viscoelastic biopolymers (versions) Method involves sterilising filtration of a bulk manufactured biopolymer by passing through a membrane which is suitable for sterilising filtration; and concentrating the sterile biopolymer by ultrafiltration to concentration of 0.8-3.0% wt/vol. Concentration of the soluble, bulk manufactured biopolymer at the sterilising filtration step is less than 0.2%. In one of the versions, the biopolymer is soluble. In one of the versions, hyaluronic acid is used as the biopolymer. |
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Method for production of microfibrillary pectin-containing cellulose fibres Invention relates to production of sugar beet fibres and may be used during production of a rheological properties regulator, a structure-forming gent and a thickener in food industry. The method envisages preparation of pulp from sugar beet pressure cake or chips with cellular tissue content no less than 18%, expressed pulp addition into a solution of an alkali reagent in softened water, water duty equal to 1:15-1:40, pH equal to 10-12, water temperature being 30-60°C. One performs fractional introduction of hydrogen peroxide with gradual temperature increase. The total quality of hydrogen peroxide taken is 25 kg of hydrogen peroxide with concentration equal to 30% per 50 kg of pressure cake/chips. When the temperature is 65-70°C one performs pH correction with 5-10% water alkali solution until pH is 9.0-10.0. Then one reduces temperature to 70-90°C within 20-45 minutes. Total bleaching time is 1-3 hours. Then one performs expression using a decanter centrifuge. Then pulp is washed, first - with softened water, then - with osmotic water. After each washing stage pulp is subjected to expression. Then one supplies finely dispersed ozone with further addition of 2.0-6.0% solution of Na2S2O3; one performs stirring and pulp expression using a decanter centrifuge. Then one performs repeated additional washing with further expression using a decanter centrifuge. As a result, a grainy wet product is produced. |
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Production method of glucan-chitosan complex from yeast biomass of brewing waste Production method of glucan-chitosan complex from yeast biomass of brewing waste involves mechanical and ultrasonic treatment of yeast biomass, destruction of proteins by treatment of the obtained suspension using alkali reagents with further extraction of a target product. As biomass, Saccharomyces living yeast is used. First, yeast is frozen to -15°C during 24 hours. After mechanical destruction, biomass is treated for 15 minutes at 20°C in an ultrasonic bath with frequency of an emitter of 35 kHz and voltage of 285 W. Biomass is acidified with chlorhydric acid till pH=5.5 and treated with ferment preparation in the amount of one pellet containing lipase - 3500 units of Ph.Eur., amylase - 4200 units of Ph.Eur. and protease - 250 units of Ph.Eur. per kilogramme of biomass in terms of dry substance; then, lipid components of yeast are removed. Fermentation is performed at t=20-29°C during 30-60 minutes. Destruction of proteins is performed at 55°C by means of a water bath during 60 minutes by treatment using 4% water solution of caustic soda at the ratio of yeast biomass and alkali, which is equal to 1:4. The medium is neutralised and hydrosol of glucan-chitosan complex is deposited by centrifugation during 10 minutes. The deposit is dried at t=55°C during 48 hours. |
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Complex of polysaccharide and hbp Present invention refers to medicine and described a complex prepared of a polysaccharide, particularly dextran, and a heparin-binding protein with the above polysaccharide being formed by (1,6) and/or (1,4) and/or (1,3) and/or (1,2) glycoside bonds and functionalised by at least one salt-forming or salt-transformed tryptophan derivative. The present invention also concerns a pharmaceutical composition containing the complex according to the invention. |
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Method of producing 2,3-dihydroxypropyl chitosan Invention relates to production of hydroxyalkyl derivatives of polysaccharides. The method of producing 2,3-dihydroxypropyl chitosan involves reacting chitosan with glycidol in the presence of hydrochloric acid with ratio glycidol:chitosan:hydrochloric acid = (2-6):1:1, at room temperature until a gel forms. The mixture is then heated at 55-65°C for 12-14 hours and the reaction mass is treated with water. The mixture is then deposited, subjected to hot extraction with water-soluble alcohols or ketones and dried. |
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Invention relates to food industry and can be used in producing pectin from vegetable material. The method involves hydrolysis and extraction of vegetable material in an electromagnetic field, separating the solid and liquid phases, concentrating, depositing pectin and drying the pectin. Hydrolysis and extraction of the vegetable material is carried out with aqueous solution of citric acid and succinic acid at temperature of 80-90°C and pH=2 in an electromagnetic field with frequency of 25-29 Hz for 55-90 minutes. The citric acid and succinic acid are taken in ratio of 3:2, respectively. Further, the material is concentrated to pectin substance concentration of 5% and coagulated with 96% ethyl alcohol for 10 minutes. The coagulate is subjected to infrared drying in a vacuum at pressure of 0.08±0.02 MPa and temperature of 35-40°C to moisture content of the end product of not more than 7%. |
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Method for silver fir wood green Method for processing silver fir wood green envisages raw material milling, the raw material treatment with an alkali solution, the raw material filtering to remove the resultant solution, acids separation by way of extraction with an organic solvent. After milling the raw material is treated with 0.1-0.5% water solution of a mineral acid at a temperature of 50±5°C with the filtered raw material subsequent two-times washing with water. Then the blended acid filtrates are concentrated by way of water boiling out in a rotor evaporator at a temperature of 60°C. Polysaccharides are sedimented from the resultant concentrate with excessive ethanol. The raw material remaining after polysaccharides extraction is subjected to treatment with an alkali and an organic solvent to separate triterpenic acids. |
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Method for production of an inuline-containing solution from girasol Method envisages washing and milling of girasol. Then girasol is subjected to extraction with acidified water during 15-20 minutes the ratio of girasol to acidified water being (1:2)÷(1:2.5). The extract is separated from the solid phase. By way of ultrafiltration, the fraction of high-molecular compounds with molecular weight over 1000 Da is separated from the extract as the target product. Then one proceeds with photosterilisation and packing. |
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Anti-cancer agents with benzophenanthridine structure and preparations containing them There are presented: using benzophenanthridine alkaloid salts for preparing therapeutic agents for treating tumours, wherein the alkaloid salt is found in the form luteic, phosphatidic or hyaluronic acid, the benzophenanthridine alkaloid salt with phosphatidic acid or hyaluronic acid, and a based pharmaceutical composition for treating tumours. |
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Invention relates to production of acidic polysaccharides and can be used in producing cosmetics. The acidic polysaccharides are selected from hyaluronic acid, chondroitin sulphate, dermatan sulphate, heparan sulphate and keratan sulphate and are characterised by simultaneous presence of alcohol groups that are esterified with butyric acid and formic acid. The method of producing said acidic polysaccharides involves dissolving an acidic polysaccharide in form of a salt with sodium or other alkali metals in formamide while heating. Butyric anhydride is then added to the obtained solution at room temperature in the presence of an organic base. The obtained homogeneous viscous reaction mixture is then diluted with aqueous NaCl solution and neutralised to pH 6-7.5. Further, the diluted reaction mixture is purified by dialysis or cross-flow filtration and the purified polysaccharide solution is frozen. The product is recovered by freeze-drying or spray-drying. In the disclosed compounds, presence of butyric acid and formic ester substitutes of a modified polymer protect from enzymatic degradation by hyaluronidases presence if tissue. |
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Group of inventions relates to biochemistry. Disclosed is a composition, having immune-stimulating properties, which contains unmodified, soluble β-glucan with average molecular weight from about 120000 Da to about 205000 Da. The composition also contains soluble β-glucan with molecular weight greater than 380000 Da in amount of less than or equal to 10% and β-glucan with molecular weight of less than 25000 Da in amount of less than or equal to 17%. The unmodified, soluble β-glucan can be used in single doses of up to about 6 mg/kg. Also disclosed is a method of producing soluble β-glucan. The method involves applying pressure of up 35 lb/in2 (24607.4 kg/m2) to a suspension of powdered β-glucan and an acid. The suspension is then heated to 135°C for a period of time which is sufficient to form soluble β-glucan. Also disclosed are compositions (versions), having immune-stimulating properties, which contain a pharmaceutically effective amount of unmodified, soluble β-glucan obtained using said method. |
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Invention relates to technical biochemistry, in particular - to determination of pectin substances quantity in vegetal raw material. "Standardised" solutions of pectin substances fractions are prepared. The fractions solutions are sampled. Saponification of pectin substances fractions in the samples of the solutions being analysed is performed with 2.5 ml of 40% NaOH solution. The pectin substances fractions are sedimented with 2.5 ml of concentrated HCl. The solutions being analysed are centrifuged in 50 ml test tubes, rotation frequency being no less than 500 rpm, during 5-7 minutes. The sediments of pectin substances fractions are suspended in distilled water in a titration cup on a magnetic stirrer for at least 30 minutes. One performs conductometric titration of the suspension of pectin substances fractions sediment, stirring. Following the conductometric titration results, graphs are drawn, relying whereon one identifies the volume of the titrant spent on pectin acid titration. Pectin substances fractions weight percentage is calculated from the formula: ω = 176 × 0,2 × V 10 × 200 × 100, where 176 - pectin acid equivalent; 0.2 - titrant normality; V - titrant volume spent on pectin acid titration, ml; 200 - volume of the solution of the corresponding pectin substances fraction taken for analysis, ml; 10 - conversion to acid milliequivalents (1 ml of 0.1n NaOH corresponding to 0.1 acid milliequivalent); 100 - percentage conversion factor. |
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Method for production of an inulin-containing solution from girasol includes tubers washing and cutting into chip-shaped slices, the latter drying and grinding and production of a suspension by way of mixing the produced flour and water. Fructosans are extracted from the suspension with the solution purified after extraction. The purified solution is cooked till the dry substances content is equal to 42-45%. Additionally proposed is an inulin-production method according whereto the purified and boiled out inulin-containing solution is passed through nanofilters with attenuation threshold equal to first 5000 Da and then - 6000 Da with separation of a solution containing inulin with molecular weight equal to 5000 - 6000 Da. The produced inulin solution undergoes crystallisation. Sedimented inulin crystals are mixed with the initial inulin-containing solution and undergo drying. Additionally one proposes fructooligosaccharides production method. |
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Barrier layer for packaging laminate and packaging laminate having said barrier layer Barrier layer is made from a hydrolysate on a cellulose base. Said layer contains lignin and oligo- or polysaccharides, where the lignin and oligo- or polysaccharides are at least partially covalently bonded to each other in a matrix and at least one auxiliary component. The auxiliary component is linked to the lignin and oligo- or polysaccharides through primary and/or second links. Said barrier layer has oxygen permeability of less than 50 cm3/m2/24 h with relative air humidity of 80%, 1 atm and thickness of less than 50 mcm. The method of making the barrier layer involves obtaining a hydrolysate on a cellulose base, which contains lignin and oligo- or polysaccharides that are partially covalently bonded to each other in a matrix, adding an auxiliary component, linking the auxiliary component with the lignin and oligo- or polysaccharides through primary and/or secondary links. The method includes a step of determining the amount of lignin and oligo- or polysaccharides and/or the number and type of functional groups per lignin and oligo- or polysaccharides in the obtained hydrolysate. |
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Invention relates to a novel chemically stable antioxidant compound which contains a lipophilic cationic moiety linked by a linking moiety to an antioxidant molecule and an anionic component for said cationic moiety, where the antioxidant compound is a mitoquinone, selected from: 10-(6'-ubiquinone)propyltriphenylphosphonium, 10-(6'-ubiquinonyl)pentyltriphenylphosphonium, 10-(6'-ubiquinonyl)decyltriphenylphosphonium, and 10-(6'-ubiquinonyl)pentadecyltriphenylphosphonium, having general formula I: or quinol form thereof, where R1, R2 and R3 denote CH3, the C atom in (C)n is saturated and n equals 3, 5, 10 or 15, and Z denotes an anionic component which is selected from a group consisting of methanesulphonate and ethanesulphonate. The invention also relates to a pharmaceutical composition for reducing oxidative stress in a cell, containing said compound and optionally containing β-cyclodextrin, a method of reducing oxidative stress in a cell and a method of producing an antioxidant compound. |
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Imidised biopolymer adhesive and hydrogel Invention refers to a recovered imidised biologically compatible polymer functionalised by an imide group. The above polymer is selected from the group consisting of polyethylene oxide, partially or completely hydrolysed by polyvinyl alcohol, polyvinylpyrrolidone, polyethyloxazoline, polyoxypropylene oxide block copolymers (poloxamers and meroxapol), polyethylene oxide and poloxamine copolymer, carboxymethyl cellulose and hydroxyalkylated cellulose, polypeptides, polysaccharides, carbohydrates, polysaccharose, hyaluronic acid, dextran, heparin sulphate, keratan sulphate, chondroitin sulphate, heparin, alginate, gelatin, collagen, albumin, ovalbumin, complex polyphosphoesters, polylactides, polyglycolides, polycaprolactones, polyamides, polyurethanes, polyesteramides, polyorthoesters, polydioxanones, polyacetals, polyketals, polycarbonates, polyorthocarbonates, polyphosphazenes, polyhydroxybutyrates, polyhydroxyvalerates, polyalkylene oxalates, polyalkylene succinates, polymaleic acids, polyamino acids, polyvinyl alcohol, polyvinylpyrrolidone, polyhydroxy cellulose, chitin, chitosan, and copolymers, ternary copolymers, or combinations or mixtures of the aforementioned materials. Also, the invention refers to a composition for a tissue adhesive, a medical device and a pharmaceutical composition. |
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Method provides for production of a wood material and its treatment by means of hydrothermic treatment with water for production of wood hydrolysate and wood remains. Used wood remains are suitable for use when making a fibrous mass. The specified hydrolysate contains oligo- and polysaccharides as the main component of the dry substance. At the same time hydrothermic treatment is carried out at the temperature in the range from 100°C to 190°C fo 10 - 360 minutes. Further the wood hydrolysate is divided into at least first and second fractions due to different molecular mass. The first fraction has a higher molecular mass compared to the second fraction. The specified first fraction is used to produce a polymer product. The produced product contains at least 80% oligo- and polysaccharides, 5-15% lignin, 0-5% monosaccharides and less than 0.1% of ash in terms of the dry substance. The specified oligo- and polysaccharides are characterised by the extent of substitution of acetyl groups (DsAC) from 0.05 to 2.0. |
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Method to produce chitosan-nucleic hydrolysate Fish roe is homogenised. Fish roe hydrolysis is carried out with a ferment preparation "Collagenase" in presence of an inhibitor for 10-12 hours. Chitosan is added to the produced hydrolysate at the ratio of 0.5-1.0:1.0. Components are mixed. |
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Present invention refers to a crossed-linked chitosan composition. The composition contains chitosan with a degree of deacetylation 30-75% and a cross-linking agent. Chitosan is randomly deacetylated. The molar ratio of the cross-linking agent to chitosan makes 0.2:1 or less at a number of functional groups in the cross-linking agent and a number of deacetylated amino groups in chitosan. The invention also represents chitosan hydrogel made of the specified composition, a method for preparing it and the versions of using it. |
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Invention relates to a sizing composition for glass fibres, particularly meant for reinforcing organic and/or inorganic matrices, the obtained glass fibres as well as composite materials containing said glass fibres. The sizing composition for glass fibres in form of a physical gel contains the following, wt %: 0.1-5 of at least one texturising agent selected from xanthanes, guar gum and succinoglycans; 2-8 of at least one film-forming agent; 0.1-8 of at least one compound selected from a group consisting of plasticisers, surfactants and dispersants; 0.1-4 of at least one binder; 0-6 of at least one additive. The subject of the invention is also glass fibres coated with said sizing composition and composite materials containing an organic or inorganic material which is reinforced with said glass fibres. |
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Method to produce water-soluble oligomer homologs of chitosans in heterogeneous system Method includes depolymerisation of a high-molecular chitosan with hydrogen peroxide. The process of chitosan depolymerisation is carried out in a double-phase system. The solid phase is activated chitosan with Mav = 450-650 kDa and the average particle size of 0.05-0.20 mm. The liquid phase is a water solution of H2O2 with concentration of H2O2 in a reaction system equal to 1-7%. The reaction is carried out for 120-180 minutes at 70°C. Then phase separation of produced chitosan homologs is carried out by means of filtration via paper or textile surface of the produced reaction mixture. The produced filtrate contains water-soluble chitosan oligomers. |
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Electrochemical method of purifying chondroitin sulphate Invention relates to a method of purifying chondroitin sulphate and can be used in food and cosmetic industry and in medicine. The method involves electrochemical deposition to obtain a hydrogel of chondroitin sulphate, stabilisation, removal from the electrode, washing and drying. The chondroitin sulphate is dissolved in a 0.01-0.1 n alkali solution in ratio of 1:50-1:200 and deposited in an alkaline medium with constant cooling and stirring. The solution is stirred at a rate of 10-20 rpm. Current density is equal to 1-10 A/m2. Voltage is preferably not lower than 2.7 V. The hydrogel of chondroitin sulphate is stabilised in a 0.05-0.5 n HCl solution. |
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Method of producing n,s-containing, chitosan-based polymer Present invention relates to a method of producing a N,S-cyclo-containing chitosan derivative. Described is a method of producing a chitosan-based N,S-cyclo-containing polymer (I) which contains in the macrochain 1-oxa-6-thia-4,8-diazocycloundecane fragments: I, by reacting chitosan with formaldehyde and a S-containing compound, characterised by that the S-containing compound used is hydrogen sulphide, the formaldehyde solution is pre-saturated with H2S and the reaction is carried out with molar ratio chitosan: formaldehyde: hydrogen sulphide of 1:2:1, at temperature of 0-60°C in a chloride medium for 24 hours. |
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Method for preparing low-molecular pectin Invention refers to methods for preparing low-molecular pectin and may be used in pharmaceutical industry for preparing new therapeutic and preventive drugs, low-molecular sorbents. The method provides pectin hydrolysis in an aqueous solution of mineral acid and liquid phase separation from an insoluble residue of pectin. The low-molecular products of pectin hydrolysis are recovered from the liquid phase by settling them in an organic solvent with water. A hydrolysis feed material is low-etherified pectin of ratio max. 30%. The hydrolysis process is continuous in a continuous-flow machine. Temperature in a working chamber of the machine is maintained at 70-100°C. Feeding speed of mineral acid into the chamber is calculated by specific formula. The prepared liquid phase is neutralized to pH min. 4.0. Thereafter, the prepared liquid phase is used to settle the low-molecular products of pectin hydrolysis. |
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Method for preparing low-molecular pectin Invention refers to methods for preparing low-molecular pectin and may be used in pharmaceutical industry for preparing new therapeutic and preventive drugs, low-molecular sorbents. The method provides pectin hydrolysis in an aqueous solution of mineral acid with heating and liquid phase separation from an insoluble residue of pectin. The low-molecular products of pectin hydrolysis are recovered from the liquid phase by settling them in an organic solvent with water. A hydrolysis feed material is low-etherified pectin of ratio max. 30%. The hydrolysis process is continuous in a continuous-flow machine. Temperature in a working chamber of the machine is maintained at 70-100°C. Feeding speed of mineral acid into the chamber is calculated by specific formula. The prepared liquid phase is exposed to additional heat treatment in a flow heat exchange following the hydrolysis process. Temperature of the additional heat treatment process coincides with hydrolysis temperature. Heat exchange section capacity is determined by specific formula. The prepared liquid phase is neutralised to pH min. 4.0. Thereafter, the prepared liquid phase is used to settle the low-molecular products of pectin hydrolysis. |
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Dextrane functionalised by hydrophobic amino acids Present invention refers to new biologically compatible polymers of dextran, namely functionalised dextran of general formula I. Dextran is functionalised by at least one residue of hydrophobic alpha-amino acid with said alpha-amino acid is cross-linked or coupled with dextran by a coupling branch R and one function F which have the values specified in the description. A residue of hydrophobic alpha-amino acid means an interaction product of amine of amino acid and acid of the coupling branch with dextran being amphiphilic at neutral pH, while a polymerisation degree of dextran makes 10 to 10000. According to one version of the implementation, hydrophobic amino acid is specified in phenylalanine, leucine, isoleucine and valine and their alcohol, amide or decarboxylated derivatives. |
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Method of reducing content of endotoxin in sample containing polysialic acid and endotoxin Invention relates to biochemistry. A base having pKa 12-13 is added to a sample and incubated for 5 minutes to 24 hours at temperature of 0-60°C. The sample is passed through an anion-exchange column, wherein polysialic acid (PSA) is adsorbed on an anion-exchange resin. The column is washed with a buffer having low ionic force, which contains NaCl in concentration of 0.2M. The polysialic acid is eluted from the column using a buffer having higher ionic force. The step for washing the column is repeated multiple times. The method may additionally include preliminary steps: neutralisation, incubation of the sample with a surfactant, a chelating agent, an organic solvent, an oxidant or peroxidase, a step for cleaning using ion-exchange, affinity, size-exclusion chromatography, using hydrophobic-interaction chromatography or combinations thereof. A sample is obtained, having low endotoxin content, wherein PSA is poly(2,8-combined sialic acid), poly(2,9-combined sialic acid), alternating 2,8-2,9-combined polysialic acid, colominic acid or an oxidised, reduced, aminated and/or hydrazide derivative thereof. To obtain a sample of culture broth containing polysialic acid and endotoxin, Escherichia coli is pre-cultured, wherein the method may include intermediate steps for removing protein, lipids, nucleic acids and/or nutrients. |
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Conjugate of hyaluronic acid and novocaine with amphoteric properties Invention refers to biochemistry. What is presented is a conjugate of hyaluronic acid and novocaine of a structure as defined in the patent claim containing 20-50% residues of novocaine. |
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Cross-linked polysaccharide and protein matrices and methods for production thereof Disclosed are versions of a method of producing cross-linked polysaccharides, involving reaction of at least one polysaccharide selected from amino-polysaccharide, amino-functionalised polysaccharide containing one or more amino groups which can be cross-linked by reducing sugar, and combinations thereof, with at least one reducing sugar. The invention also discloses polysaccharides obtained using the disclosed method, a method of producing cross-linked matrices based on polysaccharides and matrices obtained using this method. The obtained matrices may include polysaccharide matrices and composite cross-linked matrices, including polysaccharides cross-linked with proteins and/or polypeptides. |
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Method for production of pectin food extract Invention relates to food industry. The method envisages dry beet pulp swelling in 30-40°C water at a ratio of 1:20 during 30-40 minutes. Then pulp completely swollen is squeezed and twice washed with cold water. Then the washed pulp is poured with a 0.5-2.0% enzyme preparation solution with high cellulolytic activity. The weight ratio of beet pulp and the enzyme preparation is chosen within the range of 1 :(13-15). The mixture is maintained at a temperature of 40-42°C during 1-2 hours while continuously stirred. Then the mixture is separated. One pours the produced pulp with milk whey at a ratio of 1:(13-15) and performs hydrolysis extraction during 2-2.5 hours at a temperature of 95-98°C while continuously stirring. Then one performs hydrolysate separation and filtration. Then the final product is packed. |
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Gel-forming mixed dextran ester phosphates and carbamates, method for preparing them Invention refers to medicine and represents gel-forming mixed dextran esters containing phosphate and carbamate groups of general formula: {C6H7O2(OH)3-x-y{[(OP(O)ONa)mONa)]xl[(O2P(O)ONa)k]x2}x(OCONH2)y}n, wherein x=x1+x2 is a degree of substitution in phosphate groups (mono- and diesters), x=0.47-1.09; X1 is a degree of substitution in monoesters, X1=0.01-0.48; m is a number of phosphates in monoesters, m=1-2; x2 is a degree of substitution in diesters, x2=0.01-1.09; k is a number of phosphates in diesters, k=1-2; y is a degree of substitution in carbamate groups, y=0.39-1.23; n is a degree of polymerisation, 20≥n≤1000. |
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Method of producing sulphated arabinogalactan derivatives Invention relates to methods of producing arabinogalactan-based sulphated biopolymers. The method involves reaction of arabinogalactan with a sulphating SO3-pyridine complex while continuously stirring and heating, and separating the product. The arabinogalactan first undergoes mechanochemical treatment in pyridine with ratio of the mass of arabinogalactan to the volume of pyridine equal to 1:10 for 10 minutes. Sulphation is carried out at temperature 50-55°C for 40-60 minutes. The product is separated by neutralising with aqueous solution of an alkali and then precipitated in ethyl alcohol. |
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Invention refers to a new β-cyclodextrine clathrate complex (an inclusion compound) with 5-hydroxy-4-aminomethyl-1-cyclohexyl(or cycloheptyl)-3-alkoxycarbonylindole derivative: β-cyclodextrine 1:1 to 1:5, preferentially at the relation of 1:1 to 1:3 of general formula (I): wherein X means - hydrogen, chlorine, iodine, n=1 or 2, R3-C1-C3 alkyl, ALK means C1-C6 alkyl group, R1, R2 are independently specified in C1-C4-alkyl, preferentially methyl, or R1 and R2 together with a nitrogen atom (i.e. group - NR1R2) means the groups described by formulas: wherein Bn is benzyl, a Ph is phenyl with the molar ratio of 5-hydroxy-4-aminomethyl-1-cyclohexyl(or cycloheptyl)-3-alkoxycarbonylindole derivative: β-cyclodextrine 1:1 to 1:5, preferentially 1:1 to 1:3, especially preferentially in the relation of 1:2. The clathrate complex may represent nanoparticles of size not less than 100 nm. There are preferential clathrate complexes wherein 5-hydroxy-4-aminomethyl-1-cyclohexyl(or cycloheptyl)-3-alkoxycarbonylindole derivative represents 1-cyclohexyl-4-aminomethyl-5-hydroxy-2-methyl-1H-indole-3-carboxylic acid ethyl ester. The new clathrate complexes possess antiviral action and exhibit high activity versus influenza viruses. The invention also involves a pharmaceutical composition and a drug based on the clathrate complexes. Besides, the invention refers to liquid-phase and solid-phase synthesis of the clathrate complexes. |
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Method for hydrophobic modification of exopolysaccharides Disclosed is a method for hydrophobic modification of exopolysaccharides selected from xanthan and ritizan. The H+ form of exopolysaccharide is obtained. The obtained H+ form of exopolysaccharide then reacts with a primary alkyl amine in an aprotic solvent in the presence of dicyclohexyl carbodiimide. The aprotic solvent used is 1-methyl-2-pyrrolidone, dimethyl formamide and dimethylsulphoxide. The modified exopolysaccharide is separated by multiple precipitation. |
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Method for forming chitosan film coating and chitosan film coating Chitosan is dissolved in an organic acid: 4-6% citric acid or 2-8% lactic acid in the relation of the ingredients chitosan: the organic acid 1:2-1:4 to prepare a forming solution. Chitosan has molecular weight 80-500 kDa. The forming solution is added with vitamin B1 in the amount of max. 0.5 wt %. The prepared forming solution is applied on a substrate in the amount of 0.2-0.25 ml/cm2 and kept to achieve a film structure. Said method is used to form the chitosan film coating having the thickness of 50-250 mcm and the breaking elongation of 42 to 470%. |
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Inventions group relates to biotechnology. The ballast substance as a food additive for the human and animals with a monolocular stomach contains a fibre composition including the following components: lignocellulose, a fermentable fibre ingredient (bark material of soft wood, hardwood or their mixtures) and a marginally fermentable or non-fermentable fibre ingredient (fibres of soft wood, hardwood and their mixtures) at a ratio of 1:100 - 100:1, isoquinoline alkaloids. The ballast substance is produced and purified; impurities are removed at stage (a). The components are dried till dry substances content is equal to 3-12% at stage (b), milled, strained at stage (c) and mixed. The mixture is pressed at stage (d) and milled into desired size particles and mixed at stage (e). One adds a vegetable material containing isoquinoline alkaloids before pressing at stage d) and before mixing the ground components at stage (e). According to the second version of the method isoquinoline alkaloids are added at the stages of mixing (iv) or pressing and grinding at stage (v). The ballast substance is included in the composition of a fodder (in an amount of 0.01 - 50 % of the total fodder weight) and a fodder premix (up to >99%). The ballast substance is applied as a food additive in human alimentation, as a fodder or food additive for weight reduction. |
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Method of producing chondroitin sulphate from sea hydrobiont tissue Method involves preparation of material for enzymatic hydrolysis. Alkaline hydrolysis is carried out with proteolytic enzyme preparations with neutralisation of the obtained solution to pH=7. A salt is added to the obtained enzymatic hydrolysate to a value of not less than 0.1 mol/l. Successive ultrafiltration is carried out, first on a membrane with maximum retention of 50 kD with separation of high-molecular weight impurities, and then on a membrane with maximum retention of 5 kD with separation of low-molecular weight substances. The chondroitin sulphate solution retained at the membrane is washed on the same membrane with distilled water until complete removal of salts. Final washing with distilled water is carried out on a membrane with maxim retention of 50 kD. |
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Cross-linked hyaluronic acid and production method thereof Method involves activation of hyaluronic acid using a cross-linking agent and an auxiliary cross-linking agent. The activated hyaluronic acid then reacts with a nucleophilic cross-linking agent. The pH of the reaction medium ranges from 8 to 12. The nucleophilic cross-linking agent contains at least 50 wt % oligopeptide or polypeptide. Further, pH of the reaction medium is regulated to 5-7 and cross-linked hyaluronic acid is precipitated in the organic solvent. The invention also relates to use of the cross-linked hyaluronic acid obtained using this method in plastic surgery to make implants and to a hedrogel containing said cross-linked hyaluronic acid in a buffer aqueous solvent. |
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Method for producing gel-forming dextrane phosphates Invention refers to chemical-pharmaceutical industry, medicine, biotechnology, and concerns a method for producing dextrane phosphate hydrogels which can find application in preparing prolonged preparations for treating oncological diseases, infectious diseases, immune disorders. The declared invention describes the method for producing polysaccharide phosphate hydrogels, involving processing of an initial polysaccharide in mixed orthophosphoric acid and phosphorus oxide (V) in an organic solvent medium in the presence of trialkylphosphate at temperature 30-70°C that is followed by washing of the prepared products, drying and processing in an aqueous solution of a mineral compound with the etherification process of dextrane conducted in a non-polar organic solvent medium of the low boiling point specified in hydrocarbons and their derivatives and processing of the prepared product in 0.005-0.5 M aqueous solutions of sodium carbonate, or sodium hydrocarbonate, or sodium hydroxide, or their mixtures to the value pH 3.0-8.0 to be set down in ethanol and dried at temperature 20-50°C. |
Another patent 2513148.