Method of producing biologically active pectin. RU patent 2513559.
 Polysaccharide from bifidobacterium infantis strain and use thereof to treat or prevent inflammatory disorders / 2511044Group of inventions relates to biotechnology and medicine. Disclosed is a polysaccharide which is isolated from the Bifidobacterium infantis NCIMB 41003 strain and has the structure [-β(1,3)-D-GalpNAc-β(1,4)-D-Glcp-]n, where said disaccharide unit repeats n times, which yields a polysaccharide with molecular weight greater than 100000 Da. The polysaccharide exhibits immunomodulating activity and is used in preparing medicinal agents for treating or preventing undesirable inflammatory activity, undesirable gastrointestinal inflammatory activity, rheumatoid arthritis and autoimmune disorders. |
 Modified polysaccharide / 2509086Modified polysaccharide has a general formula of the type (I). The modified polysaccharide is obtained by reacting primary polysaccharide with functional groups. A zwitterion compound is formed as a result. |
 Polysaccharides containing carboxyl functional groups substituted with hydrophobic alcohol derivative / 2504554Disclosed is a polysaccharide containing carboxyl functional groups, one of which is substituted with a hydrophobic alcohol derivative. Also disclosed is a pharmaceutical composition containing one of the disclosed polysaccharides and one active ingredient. |
 Method for sterilisation by filtration of diluted viscoelastic biopolymers (versions) / 2501811Method involves sterilising filtration of a bulk manufactured biopolymer by passing through a membrane which is suitable for sterilising filtration; and concentrating the sterile biopolymer by ultrafiltration to concentration of 0.8-3.0% wt/vol. Concentration of the soluble, bulk manufactured biopolymer at the sterilising filtration step is less than 0.2%. In one of the versions, the biopolymer is soluble. In one of the versions, hyaluronic acid is used as the biopolymer. |
| Method for production of an inuline-containing solution from girasol / 2493171 Method envisages washing and milling of girasol. Then girasol is subjected to extraction with acidified water during 15-20 minutes the ratio of girasol to acidified water being (1:2)÷(1:2.5). The extract is separated from the solid phase. By way of ultrafiltration, the fraction of high-molecular compounds with molecular weight over 1000 Da is separated from the extract as the target product. Then one proceeds with photosterilisation and packing. |
 Mixed esters of butyric acid and formic acid and acidic polysaccharides and production and use thereof as skin cosmetics / 2490280Invention relates to production of acidic polysaccharides and can be used in producing cosmetics. The acidic polysaccharides are selected from hyaluronic acid, chondroitin sulphate, dermatan sulphate, heparan sulphate and keratan sulphate and are characterised by simultaneous presence of alcohol groups that are esterified with butyric acid and formic acid. The method of producing said acidic polysaccharides involves dissolving an acidic polysaccharide in form of a salt with sodium or other alkali metals in formamide while heating. Butyric anhydride is then added to the obtained solution at room temperature in the presence of an organic base. The obtained homogeneous viscous reaction mixture is then diluted with aqueous NaCl solution and neutralised to pH 6-7.5. Further, the diluted reaction mixture is purified by dialysis or cross-flow filtration and the purified polysaccharide solution is frozen. The product is recovered by freeze-drying or spray-drying. In the disclosed compounds, presence of butyric acid and formic ester substitutes of a modified polymer protect from enzymatic degradation by hyaluronidases presence if tissue. |
 Glucan compositions / 2490279Group of inventions relates to biochemistry. Disclosed is a composition, having immune-stimulating properties, which contains unmodified, soluble β-glucan with average molecular weight from about 120000 Da to about 205000 Da. The composition also contains soluble β-glucan with molecular weight greater than 380000 Da in amount of less than or equal to 10% and β-glucan with molecular weight of less than 25000 Da in amount of less than or equal to 17%. The unmodified, soluble β-glucan can be used in single doses of up to about 6 mg/kg. Also disclosed is a method of producing soluble β-glucan. The method involves applying pressure of up 35 lb/in2 (24607.4 kg/m2) to a suspension of powdered β-glucan and an acid. The suspension is then heated to 135°C for a period of time which is sufficient to form soluble β-glucan. Also disclosed are compositions (versions), having immune-stimulating properties, which contain a pharmaceutically effective amount of unmodified, soluble β-glucan obtained using said method. |
| Method for production of inulin-containing solution from girasol, inulin production method and method for production of fructooligosaccharides based on such solution / 2489445 Method for production of an inulin-containing solution from girasol includes tubers washing and cutting into chip-shaped slices, the latter drying and grinding and production of a suspension by way of mixing the produced flour and water. Fructosans are extracted from the suspension with the solution purified after extraction. The purified solution is cooked till the dry substances content is equal to 42-45%. Additionally proposed is an inulin-production method according whereto the purified and boiled out inulin-containing solution is passed through nanofilters with attenuation threshold equal to first 5000 Da and then - 6000 Da with separation of a solution containing inulin with molecular weight equal to 5000 - 6000 Da. The produced inulin solution undergoes crystallisation. Sedimented inulin crystals are mixed with the initial inulin-containing solution and undergo drying. Additionally one proposes fructooligosaccharides production method. |
 Using wood hydrolysate / 2484098Method provides for production of a wood material and its treatment by means of hydrothermic treatment with water for production of wood hydrolysate and wood remains. Used wood remains are suitable for use when making a fibrous mass. The specified hydrolysate contains oligo- and polysaccharides as the main component of the dry substance. At the same time hydrothermic treatment is carried out at the temperature in the range from 100°C to 190°C fo 10 - 360 minutes. Further the wood hydrolysate is divided into at least first and second fractions due to different molecular mass. The first fraction has a higher molecular mass compared to the second fraction. The specified first fraction is used to produce a polymer product. The produced product contains at least 80% oligo- and polysaccharides, 5-15% lignin, 0-5% monosaccharides and less than 0.1% of ash in terms of the dry substance. The specified oligo- and polysaccharides are characterised by the extent of substitution of acetyl groups (DsAC) from 0.05 to 2.0. |
 Chitosan composition / 2482133Present invention refers to a crossed-linked chitosan composition. The composition contains chitosan with a degree of deacetylation 30-75% and a cross-linking agent. Chitosan is randomly deacetylated. The molar ratio of the cross-linking agent to chitosan makes 0.2:1 or less at a number of functional groups in the cross-linking agent and a number of deacetylated amino groups in chitosan. The invention also represents chitosan hydrogel made of the specified composition, a method for preparing it and the versions of using it. |
| Method for preparing pectin from citrus husks / 2247730 Invention relates to a method for isolating pectin from vegetable raw. Method for preparing pectin from citrus husks involves hydrolysis-extraction of a solid phase and separation of a liquid phase. Before hydrolysis-extraction a solid phase is extracted with ethyl acetate and liquid acetylene successively under the above atmosphere pressure. The second extract is separated without pressure change followed by abrupt dropping pressure over a solid phase up to atmosphere value and feeding a solid phase to hydrolysis-extraction. Invention provides reducing loss of pectin being without deterioration of its organoleptic properties. |
| Method of preparing pectin from citrus squeezings / 2248360 Method envisages consecutive extraction of solid phase with acetone and liquid acetylene at superatmospheric pressure, separation of second extract at the same pressure, abruptly dropping pressure over solid phase to atmospheric value while routing solid phase to hydrolysis-extraction stage, and separation of liquid phase. |
 Method of integrated processing of larch wood to recover natural products in native state / 2249026Invention relates to processing of lignin-carbohydrate raw material, in particular larch wood, comprising reducing larch waste, contacting it with solvents, heating resulting mixture, extraction under conditions wherein components of the mixture are present in fluidized state, and recovering desired products from organic and aqueous phases. All stages are accomplished in oxygen-free atmosphere. Extraction is carried out in three steps. In the first step, wood structure in larch wood is destroyed to open fibrillar cavities, while affecting these for 3-5 min with water-hydrocarbon emulsion (n-hexane and deionized water) in the rotary-pulsating apparatuses providing fluidized state of solvent to isolate native resins, oils, and mineral salts. In the second step, dry deresined raw material is treated with absolute alcohol to recover bioflavonoids and then, in the third step, remaining dry raw material is treated with deionized water to recover carbohydrate-lignin activated biopolymer and arabinogalactane. In extraction steps 1-3, ratios of solid phase of raw material to liquid phase of reagent are the following: 1:(5-6), 1:(2.5-3), and 1:(3-3.5), respectively. In all extraction steps, separation of liquid extracts from solid extracted mass is carried out under vacuum-pulse conditions within positive temperature range below 38°C at residual pressure 0.1-5 mm Hg, performing fast vacuum development, keeping extraction mass in vacuum, rapidly releasing vacuum in an impact manner into inert medium and restoring atmospheric pressure, repeating described cycle not more that thrice. |
| Method of a complex processing of larch / 2261881 The invention is pertinent to the method of a complex processing of a larch ensuring production of a bioflavonoid complex containing 90.0-98.0 mass % of dihydroquercitin and accompanying flavonoids, and polysaccharides, which may find usage in chemical-pharmaceutical industry, food industry; fragrance industry. The method of a complex processing of larch is realized by extraction of a crushed wood with the subsequent evaporation of then extract, settling of a bioflavonoid complex at presence of water-soluble salts. Extraction of the crushed wood is conducted using isopropyl alcohol or ether-spirit mixture containing 50-80 mass % of acetic ester selected from An acetic ester sampled from ethyl-, propyl- or butyl acetate, 20-50 mass % alcohol selected from a group including ethyl alcohol, propyl alcohol, isopropyl or butyl alcohols. Separate the crushed wood from the extracting solution and settling of the bioflavonoid complex with simultaneous separation of a native pitch is conducted at presence of the water-soluble salts selected from a group includingLi2SO4,(NH4)2SO4, NH4Cl, NaCl,MgSO4, CaCl2,K2SO4, Na2SO4, or their mixtures, with the subsequent filtration of the produced bioflavonoid complex with usage of fibrous filters. The method ensures separation of the bioflavonoid complex containing 90.0-98.0 mass % of dihydroquercitin, considerably simplifies the technological process, decreases power input, excludes the cost intensive sorbents from the production process and considerably reduces a time of production the target product. |
 Polysaccharide eliciting immunostimulating activity, method for its preparing and using / 2276157Invention describes polysaccharide of the formula (I): |
 Complex of folic acid or derivative thereof, pharmaceutical composition and uses of complex / 2280650Invention relates to complex of general formula (X)n-Y, wherein X are identical or different and represent folic acid, folinic acid, dihydrofolic acid, tetrahydrofolic acid, tetrahyldopterine, pteroyl-polyglutamic acid, 2-deaminohydroxyfolic acid, 1-denitrofolic acid, 3-denitrofolic acid, 8-denitrofolic acid; Y represents polysaccharide different from arabinogalactane, selected from group containing glucanes, dextranes, polysaccharoses, fructosanes, heteropolysaccharides, homo- or heteropolysaccharide sulfates, ginseng pectin polysaccharide and other pectin polysaccharides, gumghatti, alginate, etc; or Y is selected from polyethylene oxide, methoxypolyethylene glycol and hydrophilic alcohol polymers; n >=1. Said complex permeates into in cell via pathway involving folic acid receptor on cell membrane. Complex of present invention is useful in production of pharmaceutical agents having antitumor activity. |
 Glycosaminoglycans, derivatives of k5 polysaccharide, possessing high anti-coagulant and anti-thrombosis activity and method for their preparing / 2283319Invention describes N-deacylated N-sulfatized derivative of polysaccharide K5 epimerized up to at least to 40% of the content of L-iduronic acid with respect to the total content of uronic acids and with molecular mass 2000-30000 Da and comprising 25-50% by the chain weight showing the high affinity to ATIII and possessing anti-coagulant and anti-thrombosis activity with the ratio of abovementioned activities of HCII/antiXa from 1.5 to 4. Also, invention describes a method for preparing a derivative of polysaccharide K5 comprising isolation of polysaccharide K5 from Escherichia coli cells, N-deacylation and N-sulfatization, C-5-epimerization of D-glucuronic acid to L-induronic acid, supersulfatization, selective O-desulfatization, selective 6-O-sulfatization and N-sulfatization. The reaction of C-5-epimerization is carried out by using the enzyme glucuronosyl-C-5 epimerase in the soluble or immobilized form in the presence of bivalent cations chosen from the group comprising Ba, Ca, Mg and Mn. |
 Method for production of lipopolysaccharide composition containing lipid a, lipopolysaccharide composition containing lipid a, 3-o-deactivated 4'monophosphoryl lipid a / 2302463Lipopolysaccharide composition containing lipid A is obtained by growing of culture derived from strongly rough mutent bacterial strain, conditioning thereof in stationary phase and extraction of target product from cell culture. Said composition is used for production of 3-O-deactivated 4'monophosphoryl lipid A. |
 Polysaccharide from echinacea angustifolia roots, method for production thereof and pharmaceutical composition / 2308460Invention relates to polysaccharide from Echinacea angustifolia roots with molecular mass of 1.3x109 Da and comprising of ramnose, arabinose, galactose, and galacturonic acid in ratio of 0.5:2.5:1.75:10.25. Said polysaccharide has skeleton with linear and branched repeated units, wherein linear units comprise partially acetylated and methylated galacturonic acid residues bonded via α-(1-4) bonds and branched units comprise repeated units of galacturonic acid residues and ramnose bearing side chains comprising arabinose and galactose in ratio of 2.5:1.75. Said polysaccharide is useful in production of drug for amplification of immune system. Method for production of polysaccharide includes removing of non-polysaccharide components from Echinacea angustifolia roots by extraction with solvent such as acetone or C1-C3-alcohol containing not more than 40 % v/v of water; extraction of polysaccharide fraction from Echinacea angustifolia roots directly obtained in the first stage with solvent such as water or mixture of water and acetone, or water and C1-C3-alcohol containing at least 60 % of water; polysaccharide isolating by chromatography from polysaccharide fraction obtained after preliminary purification. |
| Biomaterial, method for its preparing and its using, medicinal agent, implant and insert / 2321597 Invention describes biomaterial as a deposit comprising at least one suitable anion-active polymeric water-soluble component and amphiphilic component of ammonium type comprising a cationic surfactant. Deposit is prepared in the following steps: (1) contacting anion-active polymeric component and cyclodextrin component in aqueous medium, and (2) addition of the above said amphiphilic component of ammonium type to mixture prepared in the step (1) wherein components are taken in amounts providing formation of above said deposit. Biomaterial is used for preparing a depot-composition with regulated release and acceptable for prolonged feeding pharmaceutical substances. Also, invention describes a medicinal agent comprising biomaterial and representing a sheet material or film for closing and healing wounds, or surgery thread, and medicinal implant, or insert comprising the preliminary molded biomaterial. |
FIELD: chemistry.
SUBSTANCE: invention relates to production of polysaccharides from plant material. The method involves extracting the material with a threefold amount of 1% ammonium oxalate solution for 1.5 hours on a boiling water bath three times. The material used is oil cake obtained after separating flavonoids from common tansy flowers. The obtained extracts are mixed, filtered through several layers of gauze and pectins are precipitated in the filtrate with threefold excess 96% ethyl alcohol. The obtained precipitate is then washed successively with 96% ethyl alcohol, acetone and ether and dried over concentrated sulphurica acid.
EFFECT: invention enables to obtain biologically active pectin with anti-inflammatory action.
The invention relates to medicine and the way of obtaining biologically active substances - pectin, which has anti-inflammatory activity of waste production, "Dancehall".
The aim of the invention is the expansion of the resource base and increase efficiency of production. This goal is achieved by the fact that extraction is exposed to cheap raw materials - waste production of the drug "Dancehall"containing flavonoids from the flowers of tansy (Tanacetum vulgare) to obtain biologically active pectin, which renders anti-inflammatory action, with solubilities activity and non-toxic.
The way to obtain is the following: 0.1 kg meal obtained after the separation of flavonoids from the flowers of tansy, pour 0,3 l of 1%aqueous solution of ammonium oxalate, extracted within 1.5 hours on a boiling water bath three times. The extract is combined filtered through several layers of gauze and leachate precipitated pectins threefold excess of 96%ethyl alcohol. The precipitate is washed consistently 96%ethyl alcohol, acetone and ether, dried over concentrated sulfuric acid.
The precipitate is an amorphous substance light gray. The total output of pectin is 6.5%. Optically active. To establish a structure dedicated pectin were subjected to acid hydrolysis. Chromatographic analysis of the hydrolysate revealed the presence of glucose, ramnose, xylose and arabinose.
The analysis of the researches of biological activity showed that allocated by the above method a substance exhibits pronounced inhibitory effect on the development of exudative component of the inflammatory reaction, initiated by the formalin, and reduction of terms of treatment. So, already in the early stages of treatment of the inflammatory reaction was observed a significant reduction of edema. Complete inhibition of inflammatory reaction and restoration of the initial volume of a limb rats in the treatment of pectin was observed for the third day of the experiment. Under the influence of pectin in rats the size of mph in 4 hours after the drug was 1.6±0.2 and the level of blood on 39,91% lower than in the control group. Normalization of parameters of the ESR and the number of leukocytes was observed on day 7 of the experiment and were respectively 1,6±0.4 and 11395,8±216,8. Obvious advantage obtained by the proposed method assets in comparison with the known (calendula infusion and indometacin).
In the study of the acute toxicity obtained at the specified substances way to define LD 50 has not been possible due to the extremely low toxicity. The introduction of the pectin in the highest possible dose of 1000 mg/kg inside)determined solubility and a valid volume introduction, did not cause changes in the General condition of animals and peripheral blood. Pathological studies have not revealed in animals changes of internal organs.
Animal studies conducted in accordance with the Rules of work with the use of experimental animals (the Order of Ministry of health USSR №75 dated 12.08.1987 year) and by Russian Federal law "On protection of animals from brutal treatment" from 01.01.1997,
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A method of producing pectin, possessing biological activity, notable for expanding the resource base as a source of raw materials used meal after the separation of flavonoids from the flowers of tansy, extraction of which is held three times the number 1%-s ' solutions of ammonium oxalate for 1.5 hours on a boiling water bath three times; the obtained extracts unite, filtered through several layers of gauze and leachate precipitated pectins threefold excess of 96%ethyl alcohol; and the residue is washed consistently 96%alcohol ethanol, acetone and ether, dried over concentrated sulfuric acid.