Method to produce chitosan-nucleic hydrolysate
SUBSTANCE: fish roe is homogenised. Fish roe hydrolysis is carried out with a ferment preparation "Collagenase" in presence of an inhibitor for 10-12 hours. Chitosan is added to the produced hydrolysate at the ratio of 0.5-1.0:1.0. Components are mixed.
EFFECT: invention makes it possible to accelerate process of chitosan-nucleic complex production.
1 dwg, 1 tbl, 3 ex
The invention relates to biotechnology, in particular to methods for nucleic acid-protein hydrolysates from fish raw, and can be used in fish, meat and other food industries, as well as in medicine, biochemistry and agricultural production.
Analysis of scientific-technical and patent documentation showed that there are different ways to obtain a complex of biologically active products. Basically, it is obtaining protein hydrolysates containing biologically valuable substances, such as amino acids, deoxynucleoside, oligonucleotides and other
However, the main disadvantage of this method is that the resulting nucleic acid products have a low potential for use. Due to the fact that they have high molecular weight, transfection and delivery of these substances directly into the cell of the organism is limited. The required compaction of such molecules. This is often used cationic polymers that contribute to this process. However, use of these systems is limited due to the poor biocompatibility. Therefore, the creation of biocompatible complex polymer systems is an urgent task in the field of Biomedicine, cosmetics and other industries.
A method of obtaining peptides/amino acids from raw materials containing protein. The way which engages the grinding raw material and heating it, add water and hydrolysis using endogenous enzymes. The precondition for this hydrolysis is the use of alkaline enzymes. Optimal fermentation is carried out at pH of 7.6 to 8.2 at a temperature of 44-45°C. For termination of hydrolysis using elevated temperature above 70°C. the resulting hydrolyzate is subjected to membrane filtration for the separation of fractions of peptides/amino acids of the desired molecular weight. Hydrolizates the filtrate is concentrated to the desired level of concentration of peptides and amino acids (p. the Russian Federation No. 2333663, A23J 3/04, 2006).
The main disadvantage of this method is a multistage process and inefficient use of raw materials, because in the process of obtaining peptides/amino acids diverted valuable products of hydrolysis, in particular, DNA (deoxyribonucleic acid).
In addition, the use of alkaline environment for hydrolysis is also a negative factor, since an alkaline environment promotes the development of microflora, and you have to use UV-irradiation or the other, which complicates the production process.
A method of obtaining a product enriched in free amino acids by enzymatic hydrolysis of protein-containing raw materials. The method is carried out by enzymatic hydrolysis for 1.5 to 6.0 hours at a temperature of 35-40°C. To obtain Leche is BNO-preventive product of protein raw material is crushed and poured 5-20%water-alcohol solution at a ratio of 1:1-1:5 and alkalinized with NaOH solution to pH 8.0 to 8.5. The hydrolysis is carried out with the use of enzyme preparations in an amount of 0.1 to 1.0% by weight of raw materials. Inactivation of enzymes is carried out by heating a mixture of up to 70-80°C for 15-20 minutes. Target product produce by filtering (p. the Russian Federation No. 2171066, A23L 1/325, A23L 1/33, 2001).
The disadvantage of this method is the use of an alkaline environment, which is favorable for the development of pathogenic microflora.
In addition, continuous monitoring of the pH of the medium.
In addition, the use of high temperature for inactivation of the enzyme contributes to the further disintegration of the obtained product.
Closest to the claimed method is a method of obtaining a nucleic acid-protein hydrolysate from marine animal products, namely milk salmon and sturgeon, by enzymatic hydrolysis of raw materials by collagenase. The method involves homogenization milk and autolysis at pH 4.5 to 5.0, a temperature of 37-39°C for 46-50 hours. This gomogenizirovannom ROE is subjected to degreasing flocculation with chitosan. The autolysate is separated into a liquid fraction, which is heated up to 80°C to inactivate the enzymes, and the precipitate, which is subjected to enzymatic hydrolysis. Then the hydrolysate and liquid fraction are combined and the mixture is used as the finished product (p. the Russian Federation No. 2055482, A23J 3/04, A23J 3/00, A23J 3/34, AK 35/60, 1996).
The disadvantage JV is soba is the length of the process to obtain the hydrolysate, more than 60 hours. According to the authors of the claimed invention stage of autolysis - excessive stage of the process, because the future use of enzymatic hydrolysis, which, in principle, allows to obtain the same cleavage products in a shorter time.
The objective of the invention is to develop a method of producing chitosan-nucleic acid hydrolysate with high physiological value, but also increase the yield of low molecular weight nucleic acid product.
The problem is solved in that in the method for production of chitosan-nucleic gidrolizata the enzymatic hydrolysis is carried out in the presence of inhibitor for 10-12 hours, and the chitosan is added to the hydrolysate after hydrolysis at a ratio of 0.5 to 1.0:1.0, followed by mixing of the components.
The technical result is to accelerate the process of obtaining a nucleic acid hydrolysate.
The claimed technical result is achieved due to the fact that the hydrolysis of the raw materials are enzyme preparation "Collagenase" in the presence of inhibitor for 10-12 hours, and the chitosan is added in a ratio of 0.5 to 1.0:1.0 in the hydrolysate after hydrolysis.
To achieve the required result of hydrolysis are within 10-12 hours. To determine the optimum time of hydrolysis experiments were carried out and the results are presented in table 1.
|The content of nucleic acids during hydrolysis|
|The duration of hydrolysis, h||Withnucleic acidsmg/ml (concentration)|
These tables indicate the increase of the number of nucleic acids (nucleic acid products) with increasing duration of hydrolysis up to 12 hours At a subsequent increase in the time of hydrolysis up to 24 h, the concentration of nucleic acid products remains unchanged. Thus, practically, it was found that for the complete hydrolysis of fish ROE and allocation of the maximum number of nucleic acid products of hydrolysis enough 10-12 hours.
It was also investigated the qualitative composition of nucleic what products the resulting hydrolysis. At the end of hydrolysis were obtained following nucleic acid products: oligonucleotides, mononucleotides, oligonucleotides. Compared with the prototype already after 10-12 hours were obtained low molecular weight nucleic acid products that are better absorbed by the body, i.e. they are more physiologically valuable. To determine the qualitative composition of the hydrolysate was applied standard method of thin-layer chromatography.
The results are presented in figure 1.
The findings also suggest that the optimal duration of hydrolysis is 12 h, since the subsequent increase in the time of hydrolysis does not change the qualitative composition of the nucleic material in the hydrolysate.
Thus, it was experimentally established that the hydrolysis is carried out in 10-12 hours for maximum isolation of physiologically valuable products of hydrolysis, which are well absorbed by the body. In addition, they are easy to contact with chitosan, which provides access them directly into the cell body. We thus solve the set task of obtaining chitosan-nucleic acid complex.
To reduce the activity of the enzyme in the hydrolysis of use inhibitor, such as sorbic acid or anhydrous calcium chloride. In addition, they contribute to the suppression Mick is Flory of the hydrolysate, it allows to increase the shelf life of the product.
The claimed result is also achieved when introducing chitosan hydrolysate, i.e. after the hydrolysis step, unlike the prototype, where chitosan is used to hydrolysis, for degreasing gomogenizirovannykh Molok and after flocculation is removed from the homogenate.
In the claimed invention the chitosan contribute in the hydrolysate, which contains a complex of nucleic acid products with a negative potential, causing the molecules of chitosan bearing positively charged amino group, can form a biopolymer complex. It is this complex has potential advantages in comparison with known nucleic acid products. It can transfection of nucleic material directly into the cell of an organism that is fast enough to affect the response of the organism. Moreover, this complex protects the nucleic acid material from biodegradation during transport in the flow of biological fluid, because it is in a bound state. Since chitosan has low toxicity and good biocompatibility, the use of such a complex has great possibilities. In addition, chitosan inhibits the growth of bacteria, which helps to preserve the product.
All this leads to the conclusion that the obtained complex Hito is an-nucleic acid product can have a wide practical application.
On the basis of experiments it was established that in the hydrolysate add the chitosan in the ratio of 0,5-1,0:1,0.
Thus, if the ratio of chitosan hydrolysate is less than 0.5:1.0 then decreases its physiological value, the product loses the ability to be functional.
If the ratio of chitosan hydrolysate is more than 1.0:1.0 then deteriorate the organoleptic properties of the product, you receive astringent taste, resulting in a decrease in consumer power.
The method is as follows.
Take fish ROE, add to them the water and acetate buffer, put everything in a blender and homogenize. In the homogenate make an enzyme inhibitor and mix. The mixture is incubated for 10-12 hours. After hydrolysis, the hydrolysate contribute chitosan in the ratio of 0.5 to 1.0:1.0 and intensively stirred. If necessary, the hydrolysate may be subjected to concentration and drying.
Example 1. Take 1.0 kg of frozen milk pink, add to them 8,0 litres of water and 1.0 liter of acetate buffer to obtain a pH of 5.0. All this is placed in a blender and homogenize. In the homogenate make 0,007 kg enzyme preparation "Collagenase and 0.01 kg of sorbic acid and mixed. The mixture is incubated at a temperature of 38°C for 12 hours. Then take a 10.0 l of hydrolysate, make 1%solution of chitosan in the amount of 5.0 liters (the rate of 0.5:1.0) and intensively stirred. The product is evaporated to a volume of 1.0 liter.
Example 2. Take 1,0 kg ROE herring, add to them 8,0 litres of water and 1.0 liter of acetate buffer to pH of 5.0. The mixture is homogenized. In the homogenate make 0,007 kg enzyme preparation "Collagenase" and 0.001 kg sorbic acid and mixed. The mixture is incubated for 10 hours at a temperature of 37°C. and then hydrolyzed make a 1%solution of chitosan in the amount of 10.0 liters (ratio 1:1) and intensively stirred. The product is evaporated to a volume of 1.0 liter.
Example 3. Perform in example 1, but as raw material take chum salmon ROE. As an inhibitor take 0,02 kg of calcium chloride, and the mixture incubated for 11 hours.
The method of obtaining chitosan-nucleic acid hydrolysate, involving the hydrolysis of fish ROE enzyme preparation "Collagenase" and the use of chitosan, wherein the hydrolysis is carried out in the presence of inhibitor for 10-12 h, and chitosan is added to the hydrolysate after hydrolysis at a ratio of 0.5 to 1.0:1.0, followed by mixing of the components.
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention relates to genetic engineering and may be used in biotechnology to produce proteins of practical interest, which are a product of silent genes or low expression genes. What is presented is a method for producing a recombinant protein of human renalase 2 involving a) preparing a complete cDNA sequence, and b) expressing the prepared sequence in E.coli, wherein the stage (a) is performed by PCR amplification of each exon using specific primer pairs and a genomic DNA as a matrix, followed by twinning requiring no pre-treatment and involving the same method for adjacent exons at first, and then being the product of their amplicon combining. In this case, the primers twice exceeding exons in number are selected so that the forward primer contain a terminal sequence of the preceding exon and an initial sequence of the exon to be copied; the downstream primers contain only a sequence complementary to the end of the amplified exons, while the forward and downstream primers flanking the complete coding sequence contain restriction sites necessary for integration into a plasmid vector.
EFFECT: preparing the proteins of practical interest which are the products of silent genes or low expression genes.
2 tbl, 7 dwg, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to genetic engineering and biotechnology, and may be used in food and pharmaceutical industries. A microorganisms of the genus Corynebacterium producing 5'-inosinic acid is with provided with a capability to overexpress the genes encoding enzymes for the purine biosynthetic pathway. These enzymes include ribosephosphate-pyrophosphokinase, phosphoribosylglycinamide-formyltransferase, phosphoribosylformylglycinamidine synthetase, phosphoribosylformylglycinamidine synthetase II, phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase, inosinic acid cyclohydrolase, phosphoribosylpyrophosphate-amidotransferase and phosphoribosylaminoimidazole synthetase. A method for preparing 5'-inosinic acid involves culturing this microorganism and recovering an end product from the culture medium.
EFFECT: use of the invention provides a high yield of 5'-inosinic acid.
12 cl, 7 dwg, 1 tbl, 2 ex
SUBSTANCE: synthetic oligonucleotide primers are proposed, as well as the method to detect Lactobacillus acidophilus in starter cultures used in production of cultured milk foods. The proposed method includes performance of PCR. In case a DNA ferment is detected with size of 412 base pairs. A conclusion is made on availability of Lactobacillus acidophilus in the investigated material. The method may be used in milk processing industry for detection and identification of strains and cultures Lactobacillus acidophilus in starter cultures used in production of cultured milk foods.
EFFECT: improved efficiency of strains application.
2 cl, 1 tbl, 3 ex
SUBSTANCE: invention relates to oligonucleotide primers and the method of their use for detection of Lactobacillus delbrueckii subspecies bulgaricus in starter cultures. Proposed synthetic oligonucleotide primers have nucleotide sequences: Lbul4F 5'-GGCCAGCCAGATCGCCAGC-3' and Lbul5R 5'-GACCAGGTCGCTGTCCGGC-3'. The proposed method includes performance of PCR. In case a DNA ferment is detected with size of 409 base pairs. a conclusion is made on availability of Lactobacillus delbrueckii subspecies bulgaricus in the investigated biomaterial.
EFFECT: inventions may be used in milk processing industry for detection and identification of strains and cultures Lactobacillus delbrueckii subspecies bulgaricus in starter cultures used in production of cultured milk foods.
2 cl, 1 tbl, 3 ex
SUBSTANCE: method is proposed to produce a foaming agent. The method includes cultivation of a microorganism in a fermentative medium, the cells of which produce a foaming agent extracellularly. At the same time the fermentative medium contains a defoaming agent, which has opacity temperature. Then the defoaming agent is removed at the temperature of the fermentative medium of at least 10°C higher than the opacity temperature of the defoaming agent.
EFFECT: method makes it possible to simplify removal of a defoaming agent from a fermentative medium.
15 cl, 2 dwg, 6 tbl, 4 ex
FIELD: oil and gas industry.
SUBSTANCE: invention relates to the method to produce oil fuel, in which mixing is carried out and reaction of hydrolysis is done with water containing a ferment, which a hydrocarbon oil product, besides, water containing a ferment, is produced by means of mixing of a natural vegetable ferment, containing, at least lipase, in water. The natural vegetable ferment may additionally contain cellulase. The invention also relates to a device for production of oil fuel.
EFFECT: increased efficiency of fuel, which is stable, and also suppression of hazardous substances formation.
10 cl, 11 dwg, 1 ex
SUBSTANCE: neutral carbon source used is glucose, which is converted to aniline under the action of Escherichia coli or Streptomyces griseus bacteria. The glucose is obtained from plants. The stabiliser, vulcanisation accelerator or modified natural rubber is prepared from aniline obtained as described above.
EFFECT: invention improves environmental friendliness of methods of preparing a stabiliser, vulcanisation accelerator and modified natural rubber, which saves oil resources.
6 cl, 3 dwg, 5 ex
SUBSTANCE: invention relates to biotechnology. The method of producing 1--D-ribofuranosyl-1,2,4-triazole-3-carboxamide (ribavirin) involves reaction of excess guanosine with 1,2,4-triazole-3-carboxamide in a potassium phosphate buffer in the presence of sodium arsenate and purine nucleoside phosphorylase.
EFFECT: invention enables 100% conversion of guanosine to guanine and simplifies the process of separating the end product from the reaction mixture.
3 dwg, 1 tbl, 1 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology, particularly a method for analysing a reaction frequency of a target nucleotide sequence and one or more nucleotide sequences of concern. The method provides (a) preparing a sample of cross-linked DNA and (b) cross-linked DNA cleavage by a first restriction fragment. It is followed by (c) ligation of the cross-linked nucleotide sequences, (d) removal of the cross links; (e) nucleotide sequence cleavage by a second restriction fragment, and (f) ligation of one or more DNA sequences having a known nucleotide composition with accessible cleavage site(s) by the second restriction fragment which flanks one or more nucleotide sequences of concern. Then, (g) one or more nucleotide sequences of concern are amplified with the use of two nucleotide primers with each primer hybridised with DNA sequences which flank the nucleotide sequences of concern. It is followed by (h) hybridisation of the amplified sequence(s) with a chip; and (i) determination of the reaction frequency of the DNA sequences.
EFFECT: what is presented is the method for co-localised chromatin trapping and characterising.
29 cl, 19 dwg, 2 tbl, 8 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biotechnology and concerns oligonucleotide primers for B.mallei genetic typing. The presented primers are complementary to a differentiation fragment BMA0416 of an equinia agent genome, possess activity of upstream and downstream primers in an amplification reaction and have the following structure: 5'-TGGCGGAGTATGGATGCTG-3' - BmVAT6-Ch2s 5'-GAACGAGAACACCTACGACCTGAT-3' - BmVAT6-Ch2as.
EFFECT: presented invention may be used in practical healthcare for detection of the differentiation DNA fragment BMA0416 of the equinia agent.
3 dwg, 3 ex
SUBSTANCE: method includes depolymerisation of a high-molecular chitosan with hydrogen peroxide. The process of chitosan depolymerisation is carried out in a double-phase system. The solid phase is activated chitosan with Mav = 450-650 kDa and the average particle size of 0.05-0.20 mm. The liquid phase is a water solution of H2O2 with concentration of H2O2 in a reaction system equal to 1-7%. The reaction is carried out for 120-180 minutes at 70°C. Then phase separation of produced chitosan homologs is carried out by means of filtration via paper or textile surface of the produced reaction mixture. The produced filtrate contains water-soluble chitosan oligomers.
EFFECT: invention makes it possible to quantitatively control extent of conversion of an initial high-molecular chitosam into oligomer and low-molecular structures of its homologs.
1 tbl, 1 ex
SUBSTANCE: invention relates to a method of purifying chondroitin sulphate and can be used in food and cosmetic industry and in medicine. The method involves electrochemical deposition to obtain a hydrogel of chondroitin sulphate, stabilisation, removal from the electrode, washing and drying. The chondroitin sulphate is dissolved in a 0.01-0.1 n alkali solution in ratio of 1:50-1:200 and deposited in an alkaline medium with constant cooling and stirring. The solution is stirred at a rate of 10-20 rpm. Current density is equal to 1-10 A/m2. Voltage is preferably not lower than 2.7 V. The hydrogel of chondroitin sulphate is stabilised in a 0.05-0.5 n HCl solution.
EFFECT: invention enables to obtain chondroitin sulphate with high weight ratio of the basic substance and increases output of the end product.
5 cl, 1 ex
SUBSTANCE: present invention relates to a method of producing a N,S-cyclo-containing chitosan derivative. Described is a method of producing a chitosan-based N,S-cyclo-containing polymer (I) which contains in the macrochain 1-oxa-6-thia-4,8-diazocycloundecane fragments: I, by reacting chitosan with formaldehyde and a S-containing compound, characterised by that the S-containing compound used is hydrogen sulphide, the formaldehyde solution is pre-saturated with H2S and the reaction is carried out with molar ratio chitosan: formaldehyde: hydrogen sulphide of 1:2:1, at temperature of 0-60°C in a chloride medium for 24 hours.
EFFECT: obtaining modified chitosan which exhibits properties of a highly efficient heavy metal sorbent for waste water treatment, an extractant for separating rare, noble and precious metals and a complexing agent for biological molecules.
1 tbl, 3 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to biochemistry. What is presented is a conjugate of hyaluronic acid and novocaine of a structure as defined in the patent claim containing 20-50% residues of novocaine.
EFFECT: conjugate is water-soluble; it possess the amphoteric properties and contains no side O-acylisourea.
3 tbl, 3 ex
SUBSTANCE: disclosed are versions of a method of producing cross-linked polysaccharides, involving reaction of at least one polysaccharide selected from amino-polysaccharide, amino-functionalised polysaccharide containing one or more amino groups which can be cross-linked by reducing sugar, and combinations thereof, with at least one reducing sugar. The invention also discloses polysaccharides obtained using the disclosed method, a method of producing cross-linked matrices based on polysaccharides and matrices obtained using this method. The obtained matrices may include polysaccharide matrices and composite cross-linked matrices, including polysaccharides cross-linked with proteins and/or polypeptides.
EFFECT: obtained polysaccharides have satisfactory resistance to enzymatic degradation coupled with rheological properties of the preparation for injection, obtained matrices exhibit various physical, chemical and biological properties.
29 cl, 12 dwg, 6 tbl, 11 ex
SUBSTANCE: chitosan is dissolved in an organic acid: 4-6% citric acid or 2-8% lactic acid in the relation of the ingredients chitosan: the organic acid 1:2-1:4 to prepare a forming solution. Chitosan has molecular weight 80-500 kDa. The forming solution is added with vitamin B1 in the amount of max. 0.5 wt %. The prepared forming solution is applied on a substrate in the amount of 0.2-0.25 ml/cm2 and kept to achieve a film structure. Said method is used to form the chitosan film coating having the thickness of 50-250 mcm and the breaking elongation of 42 to 470%.
EFFECT: group of inventions allows preparing high-elastic chitosan citrate or lactate films possessing bactericidal action.
2 cl, 1 tbl, 13 ex
SUBSTANCE: method involves preparation of material for enzymatic hydrolysis. Alkaline hydrolysis is carried out with proteolytic enzyme preparations with neutralisation of the obtained solution to pH=7. A salt is added to the obtained enzymatic hydrolysate to a value of not less than 0.1 mol/l. Successive ultrafiltration is carried out, first on a membrane with maximum retention of 50 kD with separation of high-molecular weight impurities, and then on a membrane with maximum retention of 5 kD with separation of low-molecular weight substances. The chondroitin sulphate solution retained at the membrane is washed on the same membrane with distilled water until complete removal of salts. Final washing with distilled water is carried out on a membrane with maxim retention of 50 kD.
EFFECT: invention enables to obtain a chondroitin sulphate preparation with weight ratio of the basic substance.
SUBSTANCE: method involves activation of hyaluronic acid using a cross-linking agent and an auxiliary cross-linking agent. The activated hyaluronic acid then reacts with a nucleophilic cross-linking agent. The pH of the reaction medium ranges from 8 to 12. The nucleophilic cross-linking agent contains at least 50 wt % oligopeptide or polypeptide. Further, pH of the reaction medium is regulated to 5-7 and cross-linked hyaluronic acid is precipitated in the organic solvent. The invention also relates to use of the cross-linked hyaluronic acid obtained using this method in plastic surgery to make implants and to a hedrogel containing said cross-linked hyaluronic acid in a buffer aqueous solvent.
EFFECT: invention enables to obtain cross-linked hyaluronic acid in dry form, having high resistance to decomposition factors such as temperature, free radicals and enzymes.
18 cl, 3 tbl, 3 ex
SUBSTANCE: disclosed is a method of determining antibacterial properties of chitosan by estimating its minimum bacteriostatic and/or bactericidal concentration. Complex buffer solutions based on three organic acids MES, ACES and TES with different pH values are prepared. The ready buffer solutions are poured into a vessel. Double dilutions of chitosan are then prepared in vessels with the buffer solutions. Aliquots of a bacterial suspension in a fluid medium are added to the chitosan solutions in the buffer. The solutions are incubated for 24 hours at temperature which is optimum for bacterial growth. The minimum bacteriostatic and/or minimum bactericidal concentration of chitosan is then determined after incubation by determining growth of the culture or a drop in the number of living cells, respectively.
EFFECT: invention enables to determine antibacterial properties of chitosan in a wide pH range from 5,50 to 8,00 without the need to use buffers of different chemical composition.
5 dwg, 2 ex
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to a method for preparing sodium salt of hyaluronic acid modified by boron compounds with no fluid medium added. The method consists in the fact that powdered sodium salt of hyaluronic acid together with a modifying agent and mixed modifying agents is pre-homogenised in a mixer at temperature ranging within 20° to 50°C; thereafter the prepared homogenous powder mixture is simultaneously exposed to pressure and shearing deformation in a mechanochemical reactor at temperature ranging within 20° to 50°C and pressure 5-1000 MPa.
EFFECT: invention provides preparing boron-containing sodium salt of hyaluronic acid applied in boron neutron capture therapy in one-stage process parameters with no fluid medium added which requires low power, labour and water consumptions.
13 cl, 15 ex
FIELD: food industry.
SUBSTANCE: invention relates to food industry. Characterisation of the protein composition containing wheat protein partly hydrolysed by fermentative way is as follows: 20% - 80 wt % of the proteins have molecular weight equal to 25 kDa or more, 8 wt % or less have molecular weight equal to 1.4 kDa or less; the composition has nitrogen solubility index equal to 90% or more at pH 1 - 10 and hydrolysis degree equal to 3 - 8. Additionally a method for production of such composition is disclosed involving production of a suspension having hydrolysis degree equal to 3 - 8, determination of pH, correction of the suspension pH and separation of the suspension water-soluble part. The composition is applied as a milk substitute and a component of nutritive additives, sport beverages or food products including beverages.
EFFECT: invention allows to produce a composition of wheat proteins having properties identical to those of milk whey protein at least in terms of solubility and average molecular weight.
10 cl, 8 tbl, 5 ex