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Diagnostic technique for infective renal pathology

IPC classes for russian patent Diagnostic technique for infective renal pathology (RU 2539386):
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FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to a diagnostic technique for infective renal pathology caused by Chlamydia trachomatis. Substance of the method consists in gas chromatographic and mass-spectrometric urine analysis for 2-propanamide, N-amino-oxymethylamide, 2-methyl-5-(1-methyl-ethyl)-phenol and 4-4-dihydrooxy-diphenyl-sulphone, and if the measured amounts are 0.002-0.06 mmole/l; 0.002-0.06 mmole/l; 0.003-0.11 mmole/l and 0.002-0.05 mmole/l respectively, an infective renal pathology caused by Chlamydia trachomatis is diagnosed.

EFFECT: using the declared technique provides the non-invasive and informative early pre-manifestation diagnosis of the specific infective renal pathology caused by Chlamydia trachomatis.

5 ex

 

The invention relates to medicine, namely to infectious diseases, Nephrology and dermatology.

Early etiological diagnosis of specific infectious renal pathology is important to ensure the effectiveness of its treatment.

You know the identification of sediments Chlamydia trachomatis in glomerular area and localization of antigens of this type of chlamydia in the interstitial space lifetime obtained needle biopsies in patients with IgA nephropathy. The study of Chlamydia trachomatis antigen in tissue was performed using monoclonal antibodies (rakityansky I. A., Ryabov ie, the Role of Chlamydia trachomatis renal tissue in the pathogenesis of IgA nephropathy taking into account the patient's age // Journal of Infectology. - 2012. - So 4, No. 1. - S. 29-35.

The main disadvantages are the counterpart of its invasiveness and loss of use for early, before the manifestation of clinical manifestations diagnosis of specific infectious diseases of the kidney, caused by Chlamydia trachomatis.

The closest analogue prototype is the use of PCR for detection of Chlamydia trachomatis in the upper urinary tract in patients with symptoms of acute pyelonephritis. The sample for the study was taken during surgery of the upper urinary tract by aspiration of urine from the renal pelvis or ureter above the level of obstruction [Dimitrakov J., V. Ganev, Zlatanov So, Detchev I, Horvat, A., S. Kirov, Vatchkova I., D. Dimitrakov PCR studies on the presence of Chlamydia trachomatis in the upper urinary tract of patients with obstructive pyelonephritis // Folia medica. - 1998. - Vol.40, No. 3. - P. 24-28].

The main disadvantages of the prototype are its invasiveness and loss of use for early, before the manifestation of clinical manifestations diagnosis of specific infectious diseases of the kidney, caused by Chlamydia trachomatis.

The main problem to be solved by the invention is to provide a non-invasive and informative early, before the manifestation of clinical manifestations diagnosis of specific infectious diseases of the kidney, caused by Chlamydia trachomatis, using the definition in the urine of molecular markers.

The problem is solved by the fact that you define in the urine gas chromatography and mass spectrometric methods 2-propenamide, N-amino-oxymethylene, 2-methyl-5-(1-methyl-ethyl)-phenol and 4-4-dihydroxy-diphenyl-sulfon and when the amount of 0.002-0.06 mmol/l; of 0.002-0.06 mmol/l; 0.003 to 0.11 mmol/l and of 0.002-0.05 mmol/l, respectively diagnose specific infectious pathology of the kidney, caused by Chlamydia trachomatis.

As a result of our research were first identified by gas chromatography-mass spectrometry of urine parameters, showing together about the presence of infectious and chronic kidney disease caused by Chlamydia trachomatis, not only after the beginning of the manifestation of clinical manifestations of pathologists the kidneys, but before the manifestation deployed clinical manifestations of chronic kidney disease.

From the patent and technical literature and practice of examination of patients with chlamydia are not aware of the method for the diagnosis of infectious renal pathology, caused by Chlamydia trachomatis, which would be identical to declare.

Hence, the legitimate conclusion about conformity of the proposed solutions to the criterion "novelty".

The above set of essential features necessary and sufficient for obtaining a technical result - providing non-invasive and informative early, before the manifestation of clinical manifestations diagnosis of specific infectious diseases of the kidney, caused by Chlamydia trachomatis, using the definition in the urine of molecular markers.

Between the existing signs and solve problems there is a causal relationship, where each topic are needed and effect on obtaining a technical result which, together, signs sufficient to obtain it. Legitimate conclusion about conformity of the proposed technical solution the criterion of "inventive step".

The proposed method can be implemented iteratively, using the inherent essential features and, hence, the proposed solution meets the criterion of "industrial applicability".

The claimed invention aprobar the Vano for examination of patients with chlamydia. Below are the results of this testing.

Example 1. Patient K., 24 years old with a diagnosis of urogenital chlamydia (chronic cervicitis, vulvovaginitis). On the basis of the NDP-diagnostics installed infection of lower genitourinary tract Chlamydia trachomatis. When using conventional methods of diagnosis of chronic kidney disease have been identified. As a result of further examination of the patient using the proposed method are diagnosed infectious pathology of the kidneys on the basis of the combined gas chromatography and mass spectrometric detection in the urine as molecular markers of infectious and chronic kidney disease with urogenital chlamydiosis 2-propenamide in the amount of 0.05 mmol/l, N-amino-oxyethylated in the amount of 0.06 mmol/l, 2-methyl-5-(1-methyl-ethyl)-phenol in the amount of 0.11 mmol/l, 4-4-dihydroxy-diphenyl-sulfone in the amount of 0.05 mmol/l two weeks after detection in urine diagnostically significant for infectious diseases of the kidney with urogenital chlamydiosis amounts of data of molecular markers, the patient developed the first clinical manifestations of chronic kidney disease. After conducting a comprehensive etiopathogenetic treatment (systemic and local) chlamydia using software immune clinical manifestations of chronic kidney disease? is the n, diagnostically significant for infectious diseases of the kidney with urogenital chlamydiosis number of molecular markers (2-propenamide, N-amino-oxymethylene, 2-methyl-5-(1-methyl-ethyl)-phenol and 4-4-dihydroxy-diphenyl-sulfone) in urine was not determined.

Example 2. Patient K., 34 years old with a diagnosis of urogenital chlamydia (chronic cervicitis, chronic salpingoophoritis). On the basis of PCR diagnostics installed infection of lower genitourinary tract Chlamydia trachomatis. When using conventional methods of diagnosis of chronic kidney disease have been identified. As a result of further examination of the patient using the proposed method are diagnosed infectious pathology of the kidneys on the basis of the combined gas chromatography and mass spectrometric detection in the urine as molecular markers of infectious and chronic kidney disease with urogenital chlamydiosis 2-propenamide in the amount of 0.003 mmol/l, N-amino-oxyethylated in the amount of 0.002 mmol/l, 2-methyl-5-(1-methyl-ethyl)-phenol in the amount of 0.003 mmol/l, 4-4-dihydroxy-diphenyl-sulfone in the number 0,009 mmol/l one week after detection in urine diagnostically significant for infectious diseases of the kidney with urogenital chlamydiosis amounts of data of molecular markers, the patient developed clinically manifest the symptoms of chronic kidney disease. After conducting a comprehensive etiopathogenetic treatment (systemic and local) chlamydia using software immune clinical manifestations of chronic kidney disease has resolved, diagnostically significant for infectious diseases of the kidney with urogenital chlamydiosis number of molecular markers (2-propenamide, N-amino-oxymethylene, 2-methyl-5-(1-methyl-ethyl)-phenol and 4-4-dihydroxy-diphenyl-sulfone) in urine was not determined.

Example 3. Patient D., 38 years old with a diagnosis of urogenital chlamydia (chronic prostatitis). On the basis of PCR diagnostics installed infection of lower genitourinary tract Chlamydia trachomatis. When using conventional methods of diagnosis of chronic kidney disease have been identified. As a result of further examination of the patient using the proposed method are diagnosed infectious pathology of the kidneys on the basis of the combined gas chromatography and mass spectrometric detection in the urine as molecular markers of infectious and chronic kidney disease with urogenital chlamydiosis 2-propenamide in the number of 0.004 mmol/l, N-amino-oxyethylated in the number 0,006 mg/DL, 2-methyl-5-(1-methyl-ethyl)-phenol in the amount of 0,007 mmol/l, 4-4-dihydroxy-diphenyl-sulfone in the number 0,008 mmol/l After conducting a comprehensive etiopathogenetic what about the treatment (systemic and local) chlamydia using software immune diagnostically significant for infectious diseases of the kidney with urogenital chlamydiosis number of molecular markers (2-propenamide, N-amino-oxymethylene, 2-methyl-5-(1-methyl-ethyl)-phenol and 4-4-dihydroxy-diphenyl-sulfone) in urine was not determined.

Example 4. Patient P., 27 years old with a diagnosis of urogenital chlamydia (chronic prostatitis). On the basis of PCR diagnostics installed infection of lower genitourinary tract Chlamydia trachomatis. When using conventional methods of diagnosis of chronic kidney disease have been identified. As a result of further examination of the patient using the proposed method are diagnosed infectious pathology of the kidneys on the basis of the combined gas chromatography and mass spectrometric detection in the urine as molecular markers of infectious and chronic kidney disease with urogenital chlamydiosis 2-propenamide in the amount of 0.002 mmol/l, N-amino-oxyethylated in the number of 0.004 mmol/l, 2-methyl-5-(1-methyl-ethyl)-phenol in the amount of 0.004 mmol/l, 4-4-dihydroxy-diphenyl-sulfone in an amount of 0.004 mmol/l After conducting a comprehensive etiopathogenetic treatment (systemic and local) chlamydia using software immune diagnostically significant for infectious diseases of the kidney with urogenital chlamydiosis number of molecular markers (2-propenamide, N-amino-oxymethylene, 2-methyl-5-(1-methyl-ethyl)-phenol and 4-4-dihydroxy-diphenyl-sulfone) in urine did not define the camping.

Example 5. Patient E., 61 years with a diagnosis of urogenital chlamydia (chronic prostatitis). On the basis of PCR diagnostics installed infection of lower genitourinary tract Chlamydia trachomatis. When using conventional methods of diagnosis of chronic kidney disease have been identified. As a result of further examination of the patient using the proposed method are diagnosed infectious pathology of the kidneys on the basis of the combined gas chromatography and mass spectrometric detection in the urine as molecular markers of infectious and chronic kidney disease with urogenital chlamydiosis 2-propenamide in the amount of 0.06 mmol/l, N-amino-oxyethylated in the amount of 0.04 mmol/l, 2-methyl-5-(1-methyl-ethyl)-phenol in the amount of 0.04 mmol/l, 4-4-dihydroxy-diphenyl-sulfone in the amount of 0.002 mmol/L. After conducting a comprehensive etiopathogenetic treatment (systemic and local) chlamydia using software immune diagnostically significant for infectious diseases of the kidney with urogenital chlamydiosis number of molecular markers (2-propenamide, N-amino-oxymethylene, 2-methyl-5-(1-methyl-ethyl)-phenol and 4-4-dihydroxy-diphenyl-sulfone) in urine was not determined.

Thus, the examples show the advantages of the proposed technical solution in comparison with protot the POM, bringing non-invasive and informative early, before the manifestation of clinical manifestations diagnosis of specific infectious diseases of the kidney, caused by Chlamydia trachomatis, using the definition in the urine of molecular markers: 2-propenamide, N-amino-oxymethylene, 2-methyl-5-(1-methyl-ethyl)-phenol and 4-4-dihydroxy-diphenyl-sulfone.

Method for the diagnosis of infectious diseases of the kidney, caused by Chlamydia trachomatis, by identifying molecular markers, characterized in that you define in the urine gas chromatography and mass spectrometric methods 2-propenamide, N-amino-oxymethylene, 2-methyl-5-(1-methyl-ethyl)-phenol and 4-4-dihydroxy-diphenyl-sulfon and when the amount of 0.002-0.06 mmol/l; of 0.002-0.06 mmol/l; 0.003 to 0.11 mmol/l and of 0.002-0.05 mmol/l, respectively diagnose specific infectious pathology of the kidney, caused by Chlamydia trachomatis.

 

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