Diagnostic technique for traumatic microinjury of pulmonary tissue in post-mortem examination
SUBSTANCE: hose of an artificial air feed apparatus is inserted into a lobar bronchus of the estimated injured lobe of the lung. The hose is fixed by ligaturing the bronchus. The air is pumped into the examined lobe of the collapsed lung until it extends completely, and a microinjury is visualised.
EFFECT: technique can provides the more reliable diagnosis of the lung microinjury improve in dead bodies, which is achieved by filling the collapsed lung with air providing opening the microinjury.
The invention relates to medicine, namely to the court, and for the diagnosis of microdamages of lung tissue in the study of corpses of persons exposed to while living in the hospital medical manipulations - catheterization of the subclavian vein, and can be used also in other pathological conditions that led to micropolitan lung tissue.
Currently, the methods of detection of pinholes of lung tissue in the study of bodies of persons who had been in the hospital medical manipulations - catheterization of the subclavian vein, from sources of patent and scientific and technical information is unknown.
The aim of the present invention is to develop a method for the diagnosis of traumatic pinholes of lung tissue in the study of bodies of persons who had been in the hospital medical manipulations - catheterization of the subclavian vein.
This goal is achieved by a method for the diagnosis of traumatic pinholes of lung tissue in the study of bodies of persons who had been in the hospital medical manipulations - catheterization of the subclavian vein in which equity bronchus alleged damaged lobe of the lung enter the hose from the artificial air supply, the hose is fixed by ligation of bronchus by a ligature, and then produce the udachu air in the study share spaceghost light to its unfolding and opening with microsporidium.
The method is as follows. After removing organochemical have studied the corpse of a person who has suffered in the hospital medical manipulations - catheterization of the subclavian vein, bronchus sawsana lobe of the left enter the hose from the artificial air supply (pump, compressor), the hose is fixed by ligation of bronchus ligature, after which produce air flow in the study share spaceghost lung, the lung is inflated, it stretched fabric and visually detect the immediate place of pinholes.
The method is illustrated by the following examples:
Example 1. Patient M., 1989, p., "Received 01.12.2012, at 17 h 50 min in the direction of an ambulance in an emergency after about 12 h after injury with the diagnosis of intracranial injury? Coma 2-3 tbsp.". Delivered from home machine ssmf in serious condition. Injury 12 hours ago, circumstances unknown. Objectively: a serious condition and stable. Consciousness - coma 1. Skin and visible mucous membranes pale pink. Stable hemodynamics. Pulse 72 / min, rhythmic, satisfactory filling. HELL 110/70 mm RT. Art. Heart tones are clear, rhythmic. Light breathing hard, no wheezing. Percussion of the lung. Tongue moist. The abdomen is soft, painless. Chair, regular urination. Locally: bruises peri-orbital region is ti. Pupils on the right is on the left equal to 5 mm, evenly expanded. CT: hematoma frontal region with compression of the brain... 01.12.2012 year, 20.00 o'clock the State of deterioration - growing ONE. BH 12-10-8.
In the act of breathing participates auxiliary muscles. Tracheal intubation followed by mechanical ventilation. HELL 150/100 mm RT. Art. Given the severity of the patient for the organization of infusion therapy made catheterization of the subclavian vein. Without features: 01.12.2012 year, 23 h 20 min Operation: resection trepanation of the skull in the frontal region in the projection of the coronary suture with 2 sides. The massive destruction of epidural hematoma forehead... 02.12.2012 years, 0 h 30 min Standby traumatologist. Summoned to the chamber of the resuscitator. Auscultation diminished breath. It was decided to hold the pleural puncture. Pleural puncture. The patient held pleural puncture on the right under local anesthesia in the 2nd intercostal space, the resulting air conducted drainage of pleural cavity drainage No. 18 in the 2nd intercostal space. Under the pressure of the air. Drainage is fixed to the hub seam. Aseptic bandage. Active aspiration. Right-sided pneumothorax... 03.12.2012 year. Resuscitation for 30 min had no success. Found biological death 4 h 00 min final Diagnosis clinical: "Severe closed traumatic brain injury. Contusion of the Ministry of health is rather severe. Acute epidural hematoma frontal area with 2 sides with compression and dislocation of the brain. Fracture of vault of skull on the coronary suture with the transition to the skull base. Right-sided pneumothorax".
On autopsy, the left lung fills the pleural cavity completely, the right lung spaseeba, performs the pleural cavity to 1/3. Found bleeding in the parietal pleura and in the soft tissue between the right clavicle and the 1st rib between the mid-clavicle and allogrooming lines on a plot of 1.5×1.5 cm, where the center of the detected damage to the pleura size of 0.1×0.1 to see
Method for the diagnosis of traumatic pinholes of lung tissue was performed for the first time, without knowledge of the final result, and it was assumed that after inflating easy it will be placed in a container of water and the disposal of pinholes air bubbles will be reliably established localization pinholes tissue. To do this in the right upper lobar bronchus was put the hose from the artificial air supply (pump), fixed a ligature, then made the air supply. The result showed that on an inflated apical segment of the upper lobe of the detected gap pleura size of 0.3×0.2 cm at a distance of 1.8 cm down from the top, it is noted ammobates retraction of tissue (0.5×0.5 cm, a depth of 0.2 cm with CROs is izlenim pale reddish color on the plot of 0.8×0.8 cm For more convincing upper portion was immersed in water, and set of pinholes was observed intense emission of air bubbles.
Example 2. Experience: when examining the corpse K., whose death was caused by another reason, after removing organochemical was produced by the puncture of the upper lobe of the lung, and after tested the method for the diagnosis of traumatic pinholes lung tissue, the result was positive, oppresses lung tissue visually detect microsporidia lung tissue.
Thus, the proposed method for the diagnosis of traumatic pinholes of lung tissue in the study of bodies of persons who had been in the hospital medical manipulations - catheterization of the subclavian vein, allows to determine the location of the pinholes of lung tissue, the method is easy to use, reliable, on receipt of the required minimum time, and this method is practically safe for the expert and present at the opening of the medical staff. Furthermore, the method can be used in cases other pathological conditions that led to micropolitan lung tissue.
Method for the diagnosis of traumatic pinholes of lung tissue in the study of corpses of persons exposed at life in terms of the number of main telephone the Nara medical manipulations - catheterization of the subclavian vein lies in the fact that equity bronchus alleged damaged lobe of the lung enter the hose from the artificial air supply, the hose is fixed by ligation of bronchus ligature, after which produce air flow in the study share spaceghost light to its unfolding and visually detect the location of the pinholes.
SUBSTANCE: invention relates to biology, forensic and analytical chemistry and specifically to methods of determining procaine in blood plasma. The method comprises adding sodium fluoride to blood plasma containing procaine to achieve concentration of 10 mg/ml; treating the obtained mixture with acetone; separating the extract from the precipitate by filtering; evaporating acetone from the filtrate in an air current at room temperature; diluting the aqueous residue by adding water; saturating the obtained solution with ammonium sulphate; alkalising with an ammonium buffer solution to pH 9.0-9.5; extracting twice with portions of an organic extraction agent in the form of 30% camphor solution in methyl acetate, with ratio of the aqueous to the organic phase of 1:1 by volume; separating the organic extracts; combining; evaporating the solvent from the combined extract in an air current at room temperature; chromatographing the residue in a thin layer of silica gel STKH-1A on Sorbfil PTSKH-AF-A-UF plates, using a dichloromethane-ethanol mobile phase in ratio of 6:4 by volume; developing the chromatogram in UV light; eluting the analysed substance from the sorbent with a mixture of acetonitrile-methanol-0.025 M potassium dihydrogen phosphate solution with pH 3.0 in ratio of 10:10:90 by volume; chromatographing by HPLC method using a reversed-phase sorbent Nucleosil C18, a polar mobile phase acetonitrile-methanol-0.025 M potassium dihydrogen phosphate solution with pH 3.0 in ratio of 10:10:90 by volume and a UV detector; measuring optical density at wavelength 298 nm and calculating the amount of the analysed compound from the area of the chromatographic peak.
EFFECT: method improves sensitivity of determination.
3 tbl, 2 ex
SUBSTANCE: invention refers to medicine, namely to hepatology, and describes a method for examining the liver detoxification function state. The method involves measuring fasting blood AWM (average weigh molecule), ALT (alanine aminotransferase) and AST (aspartate aminotransferase), measuring the same values the night before at bedtime, as well as measuring a circulating blood volume and a packed cell volume in the morning and in the evening followed by determining a coefficient of the liver detoxification function state; and if observing the derived coefficient K≤0.6, the liver detoxification function state is considered to be satisfactory, while the coefficient K>0.6 shows the depressed liver detoxification function.
EFFECT: method provides the more reliable assessment of the liver detoxification function state.
SUBSTANCE: method involves the preliminary sorption of a specific ligand in polystyrene tray wells; the above ligand is specified in: haemoglobin, myoglobin, collagen, fibrinogen, fibronectin, immunoglobulin A; that is followed by adding a microorganism cell suspension into the tray wells with the sorbed ligand, incubating the cell suspension in the tray wells for 15 minutes, sampling a suspension aliquot from the well and adding it to wells of another or the same tray containing 0.5% sodium chloride or 0.2 M sodium phosphate; the bacterial cell adhesion is assessed by measuring a decrease of the optical density of the prepared diluted suspensions at wave length 600 nm as compared to the well references free from ligands.
EFFECT: invention is characterised by a high test rate of the microorganism adhesion to ligands of various nature, high reproducibility, using a minimum amount of the microorganism biomass, with no need for hazardous chemicals to be used.
7 tbl, 7 ex
SUBSTANCE: invention relates to biology and toxicological chemistry and can be used in practice of sanitary and epidemiological stations, chemical-toxicological, forensic and veterinary laboratories. A biological material, containing substituted 2-methoxyhydroxybenzene, is two times (each time for 30 minutes) infused with ethylacetate with mixing, separate extracts are separated from solid particles of the biological material, combined, ethylacetate is evaporated in an air flow at 18-22°C, residue is repeatedly processed with acetone, acetone extracts are separated, combined, dehydrated, evaporated in an air flow at 18-22°C, and then in a nitrogen flow until a solvent is completely removed, residue is dissolved in hexane, extracted with a buffer solution with pH 12-13, a water-alkaline extraction is separated, acidified to pH 2-3, saturated with sodium sulphate, extracted with diethyl ether, the ether extract is separated, dehydrated, evaporated in an air flow at 18-22°C, and then - in a nitrogen flow until the solvent is completely removed, residue is dissolved in a mixture of solvents hexane-dioxane-propanol-2, taken in a ratio of 20:5:1 by volume, chromatographed in macrocolumn with silicagel KSS No 3 80/120 mcm with the application of a mobile phase hexane-dioxane-propanol-2 in a ratio of 20:5:1 by volume, eluate fractions, containing the analysed substance, are combined, the eluent is evaporated first in an air flow at a temperature of 18-22°C, and then in a nitrogen flow until the solvent is completely removed, residue is dissolved in dichloromethane, processed for 20 minutes with N-tert-butyl-dimethylsilyl-N-methyltrifluoroacetamide under conditions of heating at a temperature of 60°C with carrying out determination by a chromatography-mass spectrometry method with the application of a capillary column 25 m long with an internal diameter of 0.2 mm with an immobile phase (5%-phenyl)-methylpolysiloxane, with the application of a mass-selective detector, working in an electron impact mode, an initial temperature of the column thermostat constitutes 70°C, the said temperature is kept for 3 minutes, further the temperature is programmed from 70°C to 290°C at a rate of 20°C per minute, the final column temperature is kept for 10 minutes, the injector temperature constitutes 250°C, the quadrupole temperature is 150°C, the temperature of a ion source is 230°C, the temperature of the detector interface is 300°C, intensity of a signal, conditioned by charged particles, formed in the bombardment of the analysed substance, leaving the capillary column and getting into the ion source, with a ionising beam of electrons with the energy of 70 eV, is registered, the mass-spectrum by the complete ion flow is registered and an amount of substituted 2-methoxyhydroxybenzene is calculated by the area of a chromatographic peak of its trimethylsilyl derivative.
EFFECT: achievement of an increased analysis sensitivity.
4 tbl, 3 ex
SUBSTANCE: invention relates to biology and toxicological chemistry and can be used in chemical-toxicology, expert forensic and clinical laboratories. The method includes: crushing a biological object containing N-(4-nitro-2-phenoxyphenyl)-methanesulphonamide, settling twice for 45 minutes with portions of an organic isolating agent which is methyl acetate, combining the obtained extracts, evaporating the solvent from the resultant extract, treating the residue with acetone, separating the acetone extract, evaporating the solvent from resultant extract, dissolving the residue in diethyl ether, extracting the ether solution with a buffer solution at pH 9-10, acidifying the aqueous alkaline extract with 24% hydrochloric acid to pH 2-3, saturating the obtained solution with sodium bromide, extracting with ethyl acetate, evaporating the obtained extract in an air current at 20-22°C until a dry residue is obtained, dissolving the residue in a mixture of hexane and acetone taken in volume ratio of 8:2, performing chromatography on a macrocolumn with silica gel L 40/100 mcm using a hexane-acetone mobile phase in volume ratio of 8:2, combining eluate fractions containing the analysed substance, evaporating the eluent in an air current at 20-22°C until complete removal of the solvent, dissolving the residue in methanol and performing determination via a combined physical-chemical method in the form of chromatography-mass spectrometry, using a DB-5 MS EVIDEX capillary column with a mobile phase which is 5% phenyl-95% methylpolysiloxane, using a mass-selective detector operating in electron impact mode, the initial thermostat temperature of the column is 70°C, maintaining said temperature for 3 minutes, further raising the temperature from 70°C to 290°C at a rate of 20°C per minute, maintaining the final temperature of the column for 16 minutes, the temperature of the injector is 250°C, the temperature of the quadrupole is 150°C, the temperature of the detector interface is 300°C, detecting strength of the signal resulting from charged particles formed when bombarding the analysed substance coming from the capillary column and falling into an ion source with an ionising electron beam with energy of 70 eV, recording the mass spectrum on the full ion current, while calculating the amount of N-(4-nitro-2-phenoxyphenyl)-methanesulphonamide from the area of the chromatographic peak.
EFFECT: high sensitivity of analysis.
2 ex, 3 tbl
SUBSTANCE: invention relates to medicine, namely to clinical, laboratory diagnostics, microbiological methods of research, and is aimed at standardisation of saliva analysis by method of wedge dehydration/crystallography. Claimed is method of obtaining standard, quality sample of mixed saliva facia for crystallography with application of portable laboratory device with inbuilt levels on axes X and Y, legs, regulated by height and isolating cover. From 20 to 40 microscope slides are simultaneously placed on the surface of portable laboratory device after obtaining smooth horizontal without inclination angle surface. After that, one sample of mixed saliva in amount 0.02 ml, preliminarily centrifuged for 20 min at 3000 rev/min, is applied on each microscope slide by means of micropipette. As a result a round 1.0 mm high drop of mixed saliva with diameter from 4.0 to 5.0 mm, corresponding to standard parameters, is obtained. Then, standard in dimensions drop on microscope slide placed on the surface of portable laboratory device, adjusted by levels, is dried at room temperature +18…+25°C under isolating cover for 5 hours, with further performance of microscopy of standard quality sample of saliva facia.
EFFECT: obtained sample makes it possible to interpret indices of crystallography without distortions, as standard in volume, dimensions and shape drop of mixed saliva is obtained, and there is no displacement of crystallisation centre and impairment of figures of saliva facia (crystallography pattern) in drop, dried on ideal horizontal surface of the device, ratio of central and peripheral zones of facia exactly correspond to organism's condition, which reduces percentage of false results of crystallography.
3 ex, 1 tbl, 3 dwg
SUBSTANCE: invention aims at asserting the maximum allowable blood concentrations (MAC) of heavy metals in the children living in the dirty environment as shown by health risk criteria after the chronic integrated exposure. An environmentally neglected zone is selected; a representative sampling of the children for the examination is drawn that is a basic group with using biological, social and hygienic criteria; the same criteria are used to draw a representative sampling of the children to a reference group living in the environmentally friendly zone. In the territory of the above zones, the chronic exposure of the analysed heavy metal is qualitatively assessed by establishing its average daily concentration in the ambient environment; the derived value is used to calculate a total average daily doses of a heavy metal supplied from various sources into a child's body averaged over the annual exposure for the children of both groups. Blood is sampled from the children every three months for one year to determine the content of the analysed heavy metal and also to measure the biochemical values of blood plasma and serum characterizing body responses presented by actual or potential health problems that are response markers. That is followed by calculating the average blood concentration of the analysed heavy metal and comparing it to the reference for the same heavy metal with using a Student two-sample test, thereby stating whether the children were sampled from the main and reference groups adequately. A mathematical modelling procedure is used to establish a relation between the exposure that is the total average daily doses of the analysed metal, and the exposure marker that is the average blood metal concentration. A sliding window technology is used to assert the response markers selected. The maximum allowable concentration of the exposure marker and respective marker is determined by a technique based on ratio analysis.
EFFECT: enabled measurement of the blood MAC of the heavy metals in the children after the integrated exposure with using sparing techniques making it possible to avoid a health risk.
4 tbl, 2 dwg, 1 ex
SUBSTANCE: invention represents an instant diagnostic technique for acute intestinal infections (AIIs), involving detecting indication markers of the AII aetiology with the use of laboratory immunology tests, differing by the fact that the AII aetiology is stated in children of an early age category, preferentially in the newborn children; that is accompanied by measuring the concentration of cytokine, interleukin IL-10 in coprofiltrate and diagnosing chronic placental insufficiency (CPI); a probability (P) of the bacterial AII aetiology is calculated; the value P of more than 50% testifies to the bacterial AII aetiology, while the value P being less than 50% shows the absence of the bacterial AII aetiology, and enables considering the diagnostics second stage to be necessary, which implies measuring the concentration of cytokine, interleukin IL-4 in coprofiltrate; the time of latching the newborn child to the breast is established with considering the type of feeding; that is combined with calculating a probability (P) of the viral or viral-bacterial AII aetiology, with the value P of more than 50% testifying to the viral AII aetiology, while the value being less than 50% makes it possible to state the viral-bacterial AII aetiology.
EFFECT: more accurate diagnosing of the aetiology of acute intestinal infection and simplifying the diagnostic procedure.
SUBSTANCE: polymerase chain reaction method is used to recognise polymorphous variants of IL6 and TGFb1 genes. Recognising the homozygous genotype CC in -174 position of IL6 gene in males and females, as well as the heterozygous genotype GC in -915 position of TGFb1 gene in females enables predicting the high risk of the complicated clinical course of the urogenital Chlamydial infection.
EFFECT: invention enables deciding on reasonable grounds on selecting a therapeutic approach to a specific patient suffering from urogenital Chlamydial infection in order to prevent complications of the urogenital Chlamydial infection and reproductive dysfunctions.
4 tbl, 5 ex
SUBSTANCE: invention concerns diagnosing undifferentiated connective tissue dysplasia (UCTD) in females with a personal history of miscarriage. The technique involves determining the fibrinolytic activity in an endometrial biopsy sample. If the value is less than 23.55 mm2, undifferentiated connective tissue dysplasia is diagnosed.
EFFECT: invention provides the high diagnostic accuracy and enables diagnosing UCTD in the females with the personal history of early miscarriage.
2 tbl, 2 ex
SUBSTANCE: implementing the method for prediction of caries in children involves studying a previous medical history and analysing a child's fasting non-simulated oral fluid. The previous medical history is used to acquire a child's breastfeeding length (BL), completed months, an efficiency ratio (ER) of one or more fluorine-containing, vitamin, immunomodulatory food additives and/or probiotics for the first three years of child's life; if the above are used, the efficiency ratio is assigned with a numerical value of 1, and if not used, the assigned numerical value is 0. The child's non-stimulated oral fluid is measured to determine the calcium (Ca) concentration in mMole/l and the phosphorous (P) concentration in mMole/l to be related (Ca/P), as well as the lactoferrine level (LL), mcg/ml. That is followed by calculating a prediction coefficient (PC) by formula: CP=1.44-2.28×(Ca/P)-0.07×BL-0.12×ER+0.16×LL, and if observing the condition CP≥0.7, caries of temporary teeth with uncompleted mineralisation is predicted in the child.
EFFECT: method provides the high-reliability prediction of caries of temporary teeth with uncompleted mineralisation in the child, and does not take a long time for the examination.
SUBSTANCE: invention refers to paediatric surgery and can be used for the adequate selection of surgical management of abdominal malformations in newborns. An integral assessment of the pre- and postoperative prognostic factors aims at evaluating a risk level of surgical intervention, a leukocytal intoxication index and an intraoperative coefficient. If observing a combination of the following factors: the surgical risk level I-II, the leukocytal intoxication index less than three and the intraoperative coefficient of 1 - surgical resection of the involved intestinal section is completed with the Y-shaped entero- and(or) colostomy. If observing a combination of the following factors: the surgical risk level III-IV, the leukocytal intoxication index more than three and the intraoperative coefficient of 2-3 - resection of the involved intestinal section is completed with terminal single or double entero- and(or) colostomy.
EFFECT: method enables eliminating a subjective factor in selecting surgical intervention in the newborns, and reducing a risk of postoperative complications.
4 cl, 3 tbl, 1 dwg
SUBSTANCE: invention refers to medicine, namely to neurology, can be used to specify a stage of aggravation or remission for the patients suffering multiple sclerosis (MS). The method is based on using the biochemical status values normally measured in the patient. A regression equation is used to determine the stage of multiple sclerosis: the stage of the clinical course of MS=2.5235 - 0.914006*sex+0.00355359*age+0.0702341*glucose+0.0131744*aspartate transaminase - 0.00412952*alanine transaminase - 0.0484807*bilirubin+0,153119*thymol test+0.196675*carbamide+0.00223117*cholesterol - 0.207918*amylase -0.00755805*total protein, wherein females are indicated as 0, females - as 1; the age is expressed in completed years at the moment of examination; glucose, alanine transaminase, bilirubin, thymol test, carbamide, cholesterol are absolute values measured in venous blood. If the derived value falls within the range of 2.89-3.48, the patient is stated to have remitted MS, while the value exceeding 3.48 enables diagnosing aggravated MS.
EFFECT: improved prediction procedure.
SUBSTANCE: method refers to clinical diagnostics, and aims at detecting healthy individuals suffering from non-infectious chronic diseases or a predisposition thereto by an integral assessment of risk factors, a sub-optimal health status and endothelial dysfunction. A patient is presented with the questionnaire 'The Assessment of the Sub-Optimal Health Status. SHS-25' to answer, specifies his/her smoking duration and the number of cigarettes per day. Additionally, the patient's weight, height, systolic and diastolic blood pressure, blood glucose, and total blood cholesterol are measured; the vascular wall stiffness and pulse wave reflection indices are tested with a cuff. A smoker's index, the body weight index and endothelial functional values are calculated. The derived data are electronically processed in accordance with equations. The derived greatest value enables referring the person being tested to one of five groups: the optimal health status, the sub-optimal health status of a low risk of pathological conditions, the sub-optimal health status of a high risk of pathological conditions, a cardiovascular phenotype of the sub-optimal health status of a low risk of a cardiovascular pathology, a cardiovascular phenotype of the sub-optimal health status of a high risk of a cardiovascular pathology.
EFFECT: method enables assessing the health condition of the person being tested and suffering from pre-clinical disorders by detecting and assessing the risk factors and determining the sub-optimal health status.
SUBSTANCE: instrument comprises an oral sample collection vessel, a detector able to detect a marker in this sample, an indicator actuated by a detector signal. The above vessel is detachably connected to an oral cavity instrument. The vessel comprises a sample collection element, a sample storage container, and a passage connecting the collection element and the container to supply the sample to the container by capillary action. The indicator is integrated into the container. The declared instrument is used to diagnose oral diseases by collecting the oral sample, detecting one or more markers in this sample and indicating the presence of one of the disease markers.
EFFECT: inventions enables establishing an accurate and fast diagnosis of the oral pathologies accompanying the daily oral care by placing the detector inside the container able to accumulate a required amount of the sample to be diagnosed.
25 cl, 1 dwg
SUBSTANCE: invention refers to medicine and can be used in determining the tumour sensitivity to a chemopreparation. The preparation is presented with a material taken from the tumour. The preparation is coated with a solution consisting of normal saline and the chemopreparation taken in a ratio of 4500: per a single dose of the analysed chemopreparation. That is followed by Romanowsky-Giemsa staining of the preparation 1-2 hours later. The preparation sensitivity is stated if any malignant cells are found in the preparation.
EFFECT: method provides the rapid, accessible and effective determination of the tumour sensitivity to the chemopreparation by using the optimal concentration of the chemopreparation solution and available staining.
SUBSTANCE: technique involves the clinical-laboratory examination of a sportsman who completed heavy physical activity 12-16 hours ago. The examination extent is determined taking into account the organs and systems most vulnerable to the physical activity while deriving the prognostically significant criteria of the morphofunctional body state. The examination involves measuring and analyzing the biochemical, haematological, immunological and functional values, as well as vitamin-mineral saturation. And if the above values are stably unchanged, reliably different from the norm, nonspecific changes of the sportsman's organs and systems are diagnosed.
EFFECT: technique provides the early diagnosis of the significant changes of the organs and systems during trainings and competitions that enables taking further timely measures to prevent the further progression of pathological conditions and maintaining thereby occupational performance and achieving stable high sport results.
SUBSTANCE: clinical medical history data are determined as follows: body weight index (BWI), kg/m2; waist circumference (WC), hip circumference (HC), waist-to-hip ratio, type 2 diabetes mellitus diagnosed in close relatives, arterial hypertension (AH) diagnosed. The laboratory data are measured as follows: plasminogen activator inhibitor-1 (PGAI-1), nmole/l; nitrogen oxide (NO) metabolites, %; resistin, ng/ml; insulin resistance (IR), mIU/ml; triglycerides (TG), mmole/l; high density lipoprotein cholesterol (HDLPC), mmole/l; fibrinogen, mg/dl; impaired fasting glucose (IFG), mmole/l; glycosylated haemoglobin (HbAlc), %; impaired glucose tolerance (IGT), mmole/l; homocystein (HC), mcmole/l; TNF-α, pg/ml; C-reactive protein, mg/l; endothelin and fibrinogen. The derived values are scored. The total score is used to determine a risk of atherosclerosis in the patients suffering from type 2 diabetes mellitus: extremely high, high, moderate and low. Taking into account the detected degree of risk, a dosage of aspirin and statins are determined, as well as a monitored mode of blood lipids, urinary albumin and blood creatinine is specified.
EFFECT: method enables determining a degree of risk of the atherosclerosis progression as shown by the clinical medical history and laboratory data, as well as specifying individual pathogenetic therapy for the patient that leads to reducing developing cardiovascular complications.
4 tbl, 1 ex
SUBSTANCE: invention refers to medicine, namely to surgery. A biopsy is performed, and a bipolar drain tube is inserted into the bile ducts. A terminal end of the X-ray contrast drain tube is preliminarily notched along the long axis. A recurrent biopsy is performed on the 10-14th day. The external drain tube is removed. The biopsy forceps 3.5 cm is inserted along a fistulous passage. The bile ducts are contrasted by inserting a thin X-ray contrast catheter guide. A histological material is sampled under X-ray control.
EFFECT: method for the direct punch biopsy of the tumour along the fistulous passage reduces injuries and provides the higher diagnostic accuracy.
2 ex, 9 dwg
SUBSTANCE: invention refers to medicine, and can be used to diagnose pulmonary thromboembolism. Computed-tomography pulmonary angiography is performed. Complementary scanning is performed on the 30th second from administering a contrast agent. That is followed by a post-processing preparation of the scanning findings. The preparation involves a visual assessment of the vessels and lung parenchyma taking into account the measured density of the lung parenchyma on the 30th second after administering the contrast agent in the segment of the estimated hypoperfusion or aperfusion and in the intact region at the identical level in HU. The P1/P2 relation is calculated, wherein P1 is the density of the lung parenchyma after administering the contrast agent in the pulmonary artery in the segment of the estimated hypo- or aperfusion (HU), P2 is the density of the lung parenchyma in the intact region. If the P1/P2 relation is less than 0.2, pulmonary thromboembolism is diagnosed.
EFFECT: technique provides the higher accuracy, effectiveness and information value of diagnosing pulmonary thromboembolism by complementary scanning and assessment of the relation of the density of the lung parenchyma measured at complementary scanning and in the intact region.
1 dwg, 3 tbl, 2 ex
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl