Method for detecting efficiency of apitherapy in case of ulcerous duodenal disease

FIELD: medicine, gastroenterology.

SUBSTANCE: one should introduce biologically active substance, moreover, in patient's blood serum one should detect the content of acetyl choline and choline esterase activity followed by 2-h-long intragastric pH-metry at loading with biologically active substance as warm 40-45%-honey water solution at 35-40 C, and at increased content of acetyl choline being above 1.0 mM/l, choline esterase being above 0.5 mM/l/30 min and pH level being 6.0-6.9 it is possible to consider apitherapy to be useful for treating ulcerous duodenal disease.

EFFECT: higher efficiency and accuracy of detection.

3 ex

 

The invention relates to medicine, namely to a gastroenterologist, and can be used in the treatment of peptic ulcer of the duodenum as a way of determining the effectiveness of apitherapy ulcers.

There is a method of determining the effectiveness of antibiotic therapy in the treatment of spontaneous bacterial peritonitis adopted for similar, including crops ascitic fluid on blood environment [1].

There is a method of determining the effectiveness of treatment green tea chronic gastritis and stomach cancer, adopted for the prototype, using epidemiological data [2]. The method is based on the detected feedback between the consumption of green tea and the development of chronic gastritis and stomach cancer. In addition, when both diseases were observed dose-dependent inverse relationship with increasing duration of use of green tea. However, the accuracy of the prototype method is relatively limited.

The aim of the present invention is to improve the accuracy of determination of indications for apitherapy ulcers in the duodenum.

The technical result is achieved by the fact that serum define the content of acetylcholine and cholinesterase activity, conduct a two-hour intragastric pH-metry with a load of warm 40-45%aq is m solution of honey at 35-40° And with the increasing content of acetylcholine above 1.0 mmol/l cholinesterase above 0.5 mmol/l/30 min and at pH 6,0-6,9 believe apitherapy is indicated for the treatment of peptic ulcer of the duodenum.

The method is as follows.

At admission the patient find out the availability of complaints characteristic of gastroduodenal diseases - pain syndrome localized pain in the epigastric region, addiction pain from time meal; syndromes of the gastrointestinal (nausea, vomiting, belching, heartburn, feeling of heaviness in the epigastric region) and intestinal dyspepsia (bloating, constipation or irregular stools).

Objective examination marked local tenderness in the epigastric region to the right, the resistance of the anterior abdominal wall in the upper third of the right rectus, increased levels of basal and stimulated secretion of hydrochloric acid.

In the study of blood serum to determine the level of acetylcholine in patients with peptic ulcer of the duodenum is 0.58 for 0.99 mmol/l cholinesterase - 0.08 to 0.49 mmol/l/30 min by gastroduodenoscopy there is a plague to the front (rear) wall of the bulb polygonal or angular shape, the edges of the ulcer high, smooth, clearly defined, the slopes of the ulcer crater steep. The mucous membrane around the ulcer GI is premirovan. In biopsy samples from the bottom and edges of the ulcer revealed detritus in the form of accumulations of mucus mixed with blood and slomannyh cells. In the mucous membrane around the ulcer revealed a picture of acute inflammation with edema, plasmocytomas infiltration, the expansion of blood vessels. The edges and bottom of the ulcer, there is a significant growth of connective tissue, productive endovascular, the restructuring of the glands. Held pH meter detects a pH of 2-2,5 gastric juice. Conducted introduction warm water 40-45% honey solution at a temperature of 35-40°C. for 35-45 minutes, there is an increase in pH to 6-6,5, which remains at this level for 1.5-2 hours the results Obtained with the use of honey is considered an indication for further apitherapy patient with peptic ulcer of the duodenum.

Then hold apitherapy sick for 2.5-3 weeks (without the use of blockers of the proton pump and antibiotic therapy), which allows you to view patients in clinical and laboratory remission. In particular, the pain disappears, and the syndrome, gastrointestinal dysfunction, reduced pain on palpation of the study the epigastric region.

When the control gastroduodenoscopy observed decrease periultseroznoy inflammatory shaft, the lower zone of hyperemia and edema of the mucous membrane around the ulcer. The edge is Oh inflammatory shaft is smoothed, decreases; the ulcer becomes less deep, its bottom is cleaned and covered with granulation. The morphological examination gradual filling of the ulcer granulation tissue, reduction of inflammatory infiltration and epithelialization.

Way of the following examples.

Example 1.

Patient B is 36 years old was admitted with complaints of epigastric pain, pain occurs within 3-4 hours after a meal, nausea, heartburn, feeling of heaviness in the epigastric region and bloating. Objective examination marked local tenderness in the epigastric region to the right, increased levels of basal and stimulated secretion of hydrochloric acid. During the study the serum level of acetylcholine is 0.58 mmol/l, cholinesterase - 0.08 mmol/l/30 min by gastroduodenoscopy there is a plague of the front wall of the bulb slit-like shape, the slopes of the ulcer crater steep. The mucous membrane around the ulcer hyperemic. In biopsy samples from the bottom and edges of the ulcer revealed the detritus in the mucosa around the lesion pattern of acute inflammation with the blood vessels to dilate. The edges and bottom of the ulcer is celebrated productive endovascular. Held pH meter detects a pH of 2.5 gastric juice. Conducted introduction warm aqueous 40% solution of honey when the temperature is th 35° C. for 35 minutes, there is an increase in pH to 6.5, which remains at this level for 2 hours the results Obtained with the use of honey is considered an indication for further apitherapy patient. Then hold apitherapy for 2.5 weeks (without the use of blockers of the proton pump and antibiotic therapy), which helped bring the patient in clinical and laboratory remission. Complaints of pain epigastric region does not impose syndrome gastrointestinal dysfunction not.

When the control gastroduodenoscopy there is a decrease in the zone of hyperemia and edema of the mucous membrane around the ulcer, the ulcer becomes less deep, its bottom is cleared. The morphological study noted a reduction of inflammatory infiltration and epithelialization.

Example 2.

Patient Peninsula 25 years old was admitted with complaints of epigastric pain, recurrent vomiting, bringing relief, bloating, unstable chair. An objective study noted the resistance of the anterior abdominal wall in the upper third of the right rectus. During the study the serum level of acetylcholine, the patient was 0.65 mmol/l, cholinesterase - 0.25 mmol/l/30 min by gastroduodenoscopy celebrated sore back wall of the bulb of polygonal shape, the edges of the ulcer high, smooth, clearly defined, the slopes of the tier of the steep crater. In biopsy samples from the bottom and edges of the ulcer revealed detritus mixed with leukocytes. In the mucous membrane around the ulcer revealed acute inflammation with swelling and plasmacytomas infiltration. At the bottom of the ulcer is observed the growth of connective tissue and the restructuring of the glands. Held pH meter detects the pH of 2.2 gastric juice. Conducted introduction warm water 43% honey solution at a temperature of 38°C. for 40 minutes, there is an increase in pH to 6.3, which remains at this level 110 minutes the results Obtained with the use of honey is considered an indication for further apitherapy patient. Then hold apitherapy within 20 days (without antibiotics and blockers of the proton pump), thus avoiding the pain syndrome and the syndrome of gastrointestinal dysfunction. When the control gastroduodenoscopy observed decrease periultseroznoy inflammatory shaft and edema of the mucous membrane around the ulcer. Regional inflammatory shaft is smoothed, the ulcer becomes less deep. The morphological examination gradual filling of the ulcer granulation tissue.

During the follow-up monitoring within 1 year exacerbation of peptic ulcer duodenal ulcer in this patient was not observed.

Example 3.

Sick And Skye 42 years old was admitted with complaints is mi nausea, belching, feeling of heaviness in the epigastric region, bloating, constipation. Objective examination marked local tenderness in the epigastric region, positive symptom Mendel and Vasilenko, increased levels of basal and stimulated secretion of hydrochloric acid. During the study the serum level of acetylcholine is 0.99 mmol/l cholinesterase - 0.49 mmol/l/30 min At gastroduodenoscopy study: the plague of the anterior wall of the duodenal bulb rounded, the edges of the ulcer is high, the slopes of the ulcer crater steep; the mucous membrane around the ulcer hyperemic. In the mucous membrane around the ulcer revealed a picture of acute inflammation with edema. The edges and bottom of the ulcer, there is a significant growth of connective tissue and the restructuring of the glands. Held pH meter detects pH 2 gastric juice. Conducted introduction warm water 45% honey solution at a temperature of 40°C. for 45 minutes, there is an increase in pH up to 6, which remains at this level for 1.5 hours the results Obtained with the use of honey is considered an indication for further apitherapy patient with peptic ulcer disease duodenal ulcer.

Hold apitherapy for 3 weeks without the use of blockers of the proton pump and anti H. pylori is itecture. After treatment local tenderness in the epigastric region disappeared, manifestations of the syndrome, gastrointestinal dysfunction is not marked. When the control gastroduodenoscopy there is a decrease in swelling of the mucous membrane around the ulcer, regional inflammatory shaft is smoothed, reduced; the ulcer becomes less deep. The morphological examination gradual filling of the ulcer granulation tissue and reduction of inflammatory infiltration.

According to the claimed method were treated 45 patients. In all cases, the improved accuracy of the indications for apitherapy and its effectiveness in a stand-alone treatment of peptic ulcer of the duodenum. Follow-up observation for 1-2,5 years confirmed the effectiveness of the proposed method of determination of indications for apitherapy.

Sources of information:

1. Pavlov CS How to choose an antibiotic for the treatment of spontaneous bacterial peritonitis? The wedge. the perspective. Gastroenterol., gepatol. 2002, N 3, 8-13.

2. Setiawan V.W. et al., Protective effect of green tea on the risks of choronic gastritis and stomach cancer. J. cancer. 2001; 92, 600-604.

The method of determination of efficiency of apitherapy ulcers of the duodenum, including the introduction of biologically active substances, characterized in that the serum define the content of acetylcholine and cholinesterase activity, conduct a two-hour intragastric pH-metry with a load of biologically active substance, which is used as a warm 40-45%aqueous solution of honey at 35-40°and with the increasing content of acetylcholine above 1.0 mmol/l cholinesterase above 0.5 mmol/l/30 min and at pH 6,0-6,9 believe apitherapy is indicated for the treatment of peptic ulcer of the duodenum.



 

Same patents:

FIELD: medicine, andrology.

SUBSTANCE: one should study patient's ejaculate for the content of metals and at detecting either mercury or lead being above 3.15 mcg/l on should conclude upon intoxication type of sterility. The present innovation enables to conduct differentiated diagnostics between idiopathic and intoxication types of male sterility.

EFFECT: higher accuracy of investigation.

3 ex, 1 tbl

FIELD: medicine, rheumatology.

SUBSTANCE: in patient's blood plasma one should detect the content of nitrate-ions and at their increased concentration being above 1.7 mM/l it is necessary to diagnose the debut of rheumatoid arthritis. The method enables to predict inflammation at earlier stages and could be applied in complicated, doubtful clinical situations when complete clinical picture is not available.

EFFECT: higher accuracy of diagnostics.

1 dwg, 1 ex, 1 tbl

FIELD: medicine, infectology.

SUBSTANCE: one should detect the level of terminal stable metabolites of nitrogen oxide (NOx) in whole blood. At its value ranged 39.6-86.0 mcM/l one should evaluate hemorrhagic fever accompanied with renal syndrome (HFRS) of average severity, at NOx value ranged 86.7-141.5 mcM/l - severe form of HFRS and at its values ranged 88.2-128.6 mcM/l at the background of pronounced clinical picture - as complicated disease flow. The method enables to shorten the terms for carrying out the assays.

EFFECT: higher accuracy of evaluation.

3 ex, 1 tbl

The invention relates to veterinary obstetrics

The invention relates to medicine, namely to gastroenterology

The invention relates to medicine, in particular to endocrinology

The invention relates to medicine, namely to pulmonology

The invention relates to medicine and can be used in gastroenterology

FIELD: medicine.

SUBSTANCE: method involves taking blood from ulnar vein (systemic blood circulation) and from large vein of the injured extremity proximal with respect to lesion focus (regional blood circulation). Spontaneous NST-test value is determined and difference is calculated in systemic and regional blood circulation as regional-to-systemic difference. The difference value is used for predicting clinical course of pyo-inflammatory disease in extremities.

EFFECT: high accuracy of diagnosis.

4 cl, 2 tbl

FIELD: medicine, gastroenterology.

SUBSTANCE: one should carry out diagnostic studying, moreover, on the 5th -6th d against the onset of exacerbation in case of gastric and duodenal ulcerous disease one should detect the content serotonin, histamine and acetylcholine in blood, then during 2-3 wk one should conduct medicinal therapy to detect serotonin, histamine and acetylcholine level in blood again and at serotonin content being by 2-3 times above the norm, histamine - by 1.15-1.4 times above the norm and acetylcholine - by 20-45% being below the norm one should predict the flow of gastric and duodenal ulcerous disease as a non-scarring ulcer.

EFFECT: higher accuracy of prediction.

3 ex

FIELD: medicine, diagnostics.

SUBSTANCE: the present innovation deals with genetic trials, with diagnostic field of oncological diseases due to analyzing DNA by altered status of gene methylation that take part in intracellular regulation of division, differentiating, apoptosis and detoxication processes. One should measure the status of methylation in three genes: p16, E-cadherine and GSTP1 in any human biological samples taken out of blood plasma, urine, lymph nodes, tumor tissue, inter-tissue liquid, ascitic liquid, blood cells and buccal epithelium and other; one should analyze DNA in which modified genes of tumor origin or their components are present that contain defective genes, moreover, analysis should be performed due to extracting and purifying DNA out of biological samples followed by bisulfite treatment of this DNA for modifying unprotected cytosine foundations at keeping 5-methyl cytosine being a protected cytosine foundation followed by PCR assay of bisulfite-treated and bisulfite-untreated genes under investigation and at detecting alterations obtained according to electrophoretic result of PCR amplificates, due to detecting the difference in the number and electrophoretic mobility of corresponding fractions at comparing with control methylated and unmethylated samples containing normal and hypermethylated forms of genes one should diagnose oncological diseases. The method provides higher reliability in detecting tumors, detection of remained tumor cells after operation.

EFFECT: higher efficiency of therapy.

1 cl, 3 dwg, 4 ex

FIELD: clinical medicine, pulmonology.

SUBSTANCE: one should carry out complex estimation of interleukin-1β) concentration in blood, saliva, bronchoalveolar liquid. Moreover, one should detect distribution coefficient (DC) for IL-1β as the ratio of IL-1β blood content to IL-1β salivary content. At increased IL-1β blood content by 10 times and more, by 2 times in saliva, unchanged level of bronchoalveolar IL-1β, at DC for IL-1β being above 1.0 one should predict bronchial obstruction. The method enables to conduct diagnostics of the above-mentioned disease at its earlier stages.

EFFECT: higher efficiency of prediction.

2 tbl

FIELD: medicine, juvenile clinical nephrology.

SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.

EFFECT: higher efficiency of detection.

7 dwg, 1 ex, 6 tbl

FIELD: medicine, clinical toxicology.

SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.

EFFECT: higher accuracy of prediction.

2 ex, 3 tbl

FIELD: medicine, infectology, hepatology.

SUBSTANCE: in hepatic bioptate one should detect products of lipid peroxidation (LP), such as: dienic conjugates (DC), activity of antioxidant enzymes, such as: catalase (CAT)and superoxide dismutase (SOD). One should calculate by the following formula: C = DC/(SOD x CAT)x100, where DC - the content of dienic conjugates, SOD - activity of superoxide dismutase, CAT - activity of catalase. At coefficient (C) values being above 65 one should predict high possibility for appearance of cirrhosis, at 46-645 - moderate possibility and at 14-45 -low possibility for appearance of cirrhosis.

EFFECT: higher accuracy of prediction.

3 ex

FIELD: medicine, hepatology.

SUBSTANCE: one should detect the level of hepato-specific enzymes (HSE) in blood plasma, such as: urokinase (UK), histidase (HIS), fructose-1-phosphataldolase (F-1-P), serine dehydratase (L-SD), threonine dehydratase (L-TD) and products of lipid peroxidation (LP), such as: dienic conjugates (DC), malonic dialdehyde (MDA). Moreover, one should detect the state of inspecific immunity parameters, such as: immunoregulatory index (IRI) as the ratio of T-helpers and T-suppressors, circulating immune complexes (CIC). Additionally, one should evaluate the state of regional circulation by applying rheohepatography (RHG), the system of microhemocirculation with the help of conjunctival biomicroscopy (CB) to detect intravascular index (II). In case of increased UK, HIS levels up to 0.5 mcM/ml/h, F-1-P, L-SD, L-Td, LP products, CIC by 1.5 times, higher IRI up to 2 at the norm being 1.0-1.5, altered values of regional circulation, increased II up to 2 points at the norm being 1 point, not more one should diagnose light degree of process flow. At increased level of UK, HIS up to 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 1.5-2 times, increased IRI up to 2.5, altered values of regional circulation, increased II up to 3-4 points one should diagnose average degree of process flow. At increased level of UK, HIS being above 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 2 and more times, increased IRI being above 2.5, altered values of regional circulation, increased II up to 5 points and more one should diagnose severe degree of process flow.

EFFECT: higher accuracy of diagnostics.

3 ex

The invention relates to the field of medicine

FIELD: pharmaceutical industry.

SUBSTANCE: invention provides antiinflammatory and wound-healing ointment based on natural components, which invention, along with vegetable oils, may further contain ursine fat, ursine bile, propolis, lanoline, honey, and mummified guano. Variously formulated ointments can be used for treating hemorrhoids, burns, antritis, post-stroke condition, gunshot and stab-punctured wounds, trophic ulcer, postoperative fistula, oophoritis, diabetic microangiopathy of lower extremities, as well as papillomas, birthmarks, seborrhea, and psoriasis.

EFFECT: expanded therapeutical possibilities.

6 cl, 17 ex

Up!