Method of obtaining standard quality sample of saliva facia for crystallography
SUBSTANCE: invention relates to medicine, namely to clinical, laboratory diagnostics, microbiological methods of research, and is aimed at standardisation of saliva analysis by method of wedge dehydration/crystallography. Claimed is method of obtaining standard, quality sample of mixed saliva facia for crystallography with application of portable laboratory device with inbuilt levels on axes X and Y, legs, regulated by height and isolating cover. From 20 to 40 microscope slides are simultaneously placed on the surface of portable laboratory device after obtaining smooth horizontal without inclination angle surface. After that, one sample of mixed saliva in amount 0.02 ml, preliminarily centrifuged for 20 min at 3000 rev/min, is applied on each microscope slide by means of micropipette. As a result a round 1.0 mm high drop of mixed saliva with diameter from 4.0 to 5.0 mm, corresponding to standard parameters, is obtained. Then, standard in dimensions drop on microscope slide placed on the surface of portable laboratory device, adjusted by levels, is dried at room temperature +18…+25°C under isolating cover for 5 hours, with further performance of microscopy of standard quality sample of saliva facia.
EFFECT: obtained sample makes it possible to interpret indices of crystallography without distortions, as standard in volume, dimensions and shape drop of mixed saliva is obtained, and there is no displacement of crystallisation centre and impairment of figures of saliva facia (crystallography pattern) in drop, dried on ideal horizontal surface of the device, ratio of central and peripheral zones of facia exactly correspond to organism's condition, which reduces percentage of false results of crystallography.
3 ex, 1 tbl, 3 dwg
The invention relates to medicine, namely clinical laboratory diagnosis, microbiological methods, and aims at standardizing research saliva method wedge dehydration/crystallography.
Study of physico-chemical and metabolic parameters of saliva available biological fluids non-invasive methods, is becoming increasingly important. Currently increasingly important method wedge dehydration/crystallography saliva, which was opened by the researcher that is, rapes. Russian scientists Century. N. Shabalin and S. N. Shatokhina revealed the main regularities of the formation of structures of the solid phase bioliquids, classified and given an objective description of their system and local morphological features (barer, M., Denisov A. B., Evocative I. P., I. Mikhaleva N. / Crystallization oral fluid. The composition and frequency of the substrate surface //. Bulletin of experimental biology and medicine, 2007. - 12. - S. 693-696). Implementation of the method is that when drying a drop of biological fluid is formed dry film or facies, representing structural macropore dry residue, which is clearly distinguished by the shape, orientation, size, and other parameters figure facies (solid residue of saliva). The importance of the first factors determine the nature of structure formation of saliva, water and the total concentration of dissolved mineral and organic substances, as well as their relationship.
There is a way wedge dehydration/crystallography mixed with saliva, developed by the Russian scientists Century. N. Shabalina and S. N. Athinai (, Moscow 2001).
But the disadvantages of the prototype are:
- applying drops of investigated liquid on a glass slide uncertain size, making it difficult to identify and leads to bias data;
- drying drops of the liquid under study on the slide is held in thermostat, which leads to a rapid, uneven drying the droplets to the distortion figures facies saliva and, as a result, false result;
- no thermostat flat horizontal surface does not allow the drop evenly to dry, which leads to distortion ratio of the Central and peripheral zones, facies - the main parameter of the study;
- hit probability in a drying drop of foreign particles due to the absence of a protective insulating coating causes artifacts facies of saliva.
The goal is to get a quality standard sample facies mixed with saliva for studies wedge dehydration / crystallography.
The technical result consists in the fact that mo is to get a quality standard facies mixed with saliva for crystallography uses a portable laboratory with built-in levels on the X and Y axes, height-adjustable legs and an insulating cover.
The technical result is achieved by the fact that at the stage of preparation of saliva sample for deposition on a glass slide and further drying is used, the device is portable laboratory with an insulating cover. Pre-aligned horizontal surface of the device is portable laboratory using the adjustable legs under the control of the built-in surface levels on the X-axis and y After receiving a flat horizontal without error angle of inclination of the plane on the surface of the device portable laboratory are put slides from 20 to 40 pieces at a time. Then with the help of micropipette "Inpipe" on each glass slide is applied to one sample of mixed saliva in the amount of 0.02 ml, previously centrifuged for 20 min at 3000 rpm In the relevant standard settings drop mixed with saliva: height drops of 1.0 mm diameter drops from 4.0 to 5.0 mm, the shape of the drop rounded without distorting the form. Next standard size drop on a glass slide, placed on the surface of the portable device laboratory, configured, dried at room temperature +18...+25°C under an insulating lid for 5 hours, then is he who microscopy standard quality sample facies saliva. Thus, the resulting model allows us to interpret the indicators of crystallography without distortion, because the obtained standard volume, size and shape of a drop mixed with saliva, and drying the droplets is carried out on a perfect horizontal surface of the device, resulting in no displacement of the centre of crystallization, the violation of figures facies saliva (figure crystallography), and the ratio of the Central and peripheral zones facies correspond exactly to the condition of the body, which reduces the percentage of false positives crystallography.
Used portable laboratory is shown in Fig.1, where the index 1 - shelf extruded acrylic glass, the pointer 2 levels on the X-axis and Y-index 3 - adjustable legs, index 4 - slides, index 5 - insulating cover, which prevents the influence of the external environment and maintains a constant humidity and temperature, 6 - hole insulating cover.
Inside portable laboratory has two levels, located at right angles relative to each other. Using layers, you can estimate the inclination of the surface relative to the horizon along the X-axis and y-axis When placing the level on the X-axis and Y-axis bubble inside should be placed in a Central position. The scheme of obtaining high-quality with andariego sample facies saliva for crystallography is shown in Fig.2, where a is the standard sample facies mixed with saliva.
After fixing the parameters necessary to obtain a flat surface using a portable laboratory device on a glass slide by using the pipettor is applied to 0.02 ml of mixed saliva. Get a drop of mixed saliva with standard parameters: height drops of 1.0 mm, diameter 5.0 mm, the shape of the drop rounded without distorting the form, and when it is drying, get a sample of facies saliva without displacement of the center of crystallization and without breaking figures facies, the ratio of the Central and peripheral zones corresponds to the condition of the body.
In the case when the surface alignment is not carried out through a level, get a drop of mixed saliva with custom parameters: height drops of 1.0 mm, a diameter of 10.0 mm, the shape of the drop is deformed edge, distorted in shape, and when it is drying receive a custom sample facies saliva with offset center of crystallization and a violation of the texture picture facies without the ability to determine the ratio of the Central and peripheral zones, which results in a false result in the diagnosis of that shown in Fig.3, where B is a distorted sample facies of saliva, which is impossible to investigate the method of crystallography.
The advantage of the proposed method consists in the following:<> - leveling horizontal surface by means of additional equipment levels on the X and Y axes in the portable laboratory allows to get a little bit mixed with saliva, the corresponding standard parameters: height drops of 1.0 mm, a diameter of from 4.0 to 5.0 mm, the shape of the drop rounded without distortion, and after drying receive the sample facies saliva without displacement of the center of crystallization and without damaging the texture of the picture facies saliva, where the ratio of the Central and peripheral zones visibile and corresponds to the state of the organism.
- the use of an insulating cover device of a portable laboratory during the drying drops mixed with saliva prevents the ingress of foreign particles into the drop, which avoids artifacts.
- reducing the number of false results if the instrument is portable laboratory reaches 50%. The results provided in table 1.
the method is technically available and requires no special skills and labor costs.
Child B., age 9, with a compensated form of caries, from the category of healthy children, with the index of the CPU 1, Hygienic index on green-Vermilion 0.2 points, with good oral hygiene. Using sterile swabs was extracted oral fluid in the amount of 2.0 ml and then receiving the receiving facies saliva from 0,02 ml drops mixed with saliva, in accordance with the proposed method applied on a glass slide and drying with the use of portable laboratory device. Received standard quality sample facies saliva height 1.0 mm, diameter 4.0 mm, form smooth rounded without distortion, and the standard sample facies saliva without displacement of the center of crystallization and without damaging the texture of the picture facies. For the Central zone is characterized by a clear illustration of the formation of large elongated kristallograficheskih structures coming from the center drops, fused among themselves and with the tree and pteridophyte form. The ratio of the following areas: the Central area is 70%, the peripheral zone is 30%. The obtained data indicate the absence of General pathology and pathology of the oral cavity that corresponds to the data records.
Child Of N., 12 years with decompensated form of caries, from the category of frequently ill children, the index of the CPU 9, hygienic index on the green-Vermilion 2.3 points with poor oral hygiene. Using sterile swabs was extracted mixed with saliva in the amount of 2.0 ml, and then the subsequent figures facies saliva from 0,02 ml drops mixed with saliva on the method proposed by: C. N. Shabalina, S. N. Athinai, 2001, without using the device as a portable laboratory. In R. the result obtained nonstandard saliva sample height 1.0 mm, diameter of 10.0 mm, the shape of the drop is deformed edge, distorted, and after drying in an incubator received a non-standard sample facies saliva with offset center of crystallization and a violation of the texture picture facies, which made it impossible to identify the important parameter in the diagnosis is the ratio of the Central and peripheral zones, which contributed to obtaining false results.
Child Of N., 12 years with decompensated form of caries, from the category of frequently ill children, the index of the CPU 9, hygienic index on the green-Vermilion 2.3 points with poor oral hygiene. Using sterile swabs was extracted oral fluid in the amount of 2.0 ml, and then the subsequent figures facies saliva from 0,02 ml drops of mixed saliva in accordance with the proposed method. At the beginning of the received standard quality saliva sample height 1.0 mm, diameter 5.0 mm, smooth round shape, and after drying using the device received the standard sample facies saliva without displacement of the center of crystallization and without damaging the texture of the picture facies. The Central zone consists of amorphous pattern over the entire area drops reviewed a large number isometrically located crystalline structures stellate and irregular shape. Identifies the self destruction of the crystals of the picture. The ratio of areas in percents: Central area 90%, the peripheral area is 10%. Indicating that somatic disorders in a patient.
|The method of obtaining standard quality sample facies saliva for crystallography|
|Method of crystallography||The number of studied facies saliva (abs. numbers)||The number of unsatisfactory results (abs. numbers)||The number of unsatisfactory results (in percentages)||The probability P|
|Crystallography saliva method prototype (C. N. Shabalin, S. N. Shatokhina, , Moscow, 2001)||300||190||63%±m||<0,05|
|Crystallography of saliva by the proposed method with the use of portable devices laboratory "Crystallina"||300||50||16,6%±m||<0,05|
The method of obtaining standard quality sample facies of saliva in a crystal is ographie, includes a sampling of mixed saliva in a sterile tube with a volume of 2.0 ml, followed by the application of drops mixed with saliva on a glass slide and drying, characterized in that the mixed saliva pre-centrifuged for 20 minutes at 3000 rpm, it takes a set amount of 0.02 ml was centrifuged saliva using a micropipette and applied on a glass slide, placed on the surface of the portable device laboratory, established at levels X and Y, relative to the horizon, with the receipt of standard shape and size drops mixed with saliva height of 1.0 mm, a diameter of from 4.0 to 5.0 mm, round shape, in a further drop also dried on the surface of the portable device laboratory at room temperature +18...+25°C under an insulating lid for 5 hours to obtain figures facies saliva without distortion of the crystal pattern and without displacement of the center of crystallization.
SUBSTANCE: invention aims at asserting the maximum allowable blood concentrations (MAC) of heavy metals in the children living in the dirty environment as shown by health risk criteria after the chronic integrated exposure. An environmentally neglected zone is selected; a representative sampling of the children for the examination is drawn that is a basic group with using biological, social and hygienic criteria; the same criteria are used to draw a representative sampling of the children to a reference group living in the environmentally friendly zone. In the territory of the above zones, the chronic exposure of the analysed heavy metal is qualitatively assessed by establishing its average daily concentration in the ambient environment; the derived value is used to calculate a total average daily doses of a heavy metal supplied from various sources into a child's body averaged over the annual exposure for the children of both groups. Blood is sampled from the children every three months for one year to determine the content of the analysed heavy metal and also to measure the biochemical values of blood plasma and serum characterizing body responses presented by actual or potential health problems that are response markers. That is followed by calculating the average blood concentration of the analysed heavy metal and comparing it to the reference for the same heavy metal with using a Student two-sample test, thereby stating whether the children were sampled from the main and reference groups adequately. A mathematical modelling procedure is used to establish a relation between the exposure that is the total average daily doses of the analysed metal, and the exposure marker that is the average blood metal concentration. A sliding window technology is used to assert the response markers selected. The maximum allowable concentration of the exposure marker and respective marker is determined by a technique based on ratio analysis.
EFFECT: enabled measurement of the blood MAC of the heavy metals in the children after the integrated exposure with using sparing techniques making it possible to avoid a health risk.
4 tbl, 2 dwg, 1 ex
SUBSTANCE: invention represents an instant diagnostic technique for acute intestinal infections (AIIs), involving detecting indication markers of the AII aetiology with the use of laboratory immunology tests, differing by the fact that the AII aetiology is stated in children of an early age category, preferentially in the newborn children; that is accompanied by measuring the concentration of cytokine, interleukin IL-10 in coprofiltrate and diagnosing chronic placental insufficiency (CPI); a probability (P) of the bacterial AII aetiology is calculated; the value P of more than 50% testifies to the bacterial AII aetiology, while the value P being less than 50% shows the absence of the bacterial AII aetiology, and enables considering the diagnostics second stage to be necessary, which implies measuring the concentration of cytokine, interleukin IL-4 in coprofiltrate; the time of latching the newborn child to the breast is established with considering the type of feeding; that is combined with calculating a probability (P) of the viral or viral-bacterial AII aetiology, with the value P of more than 50% testifying to the viral AII aetiology, while the value being less than 50% makes it possible to state the viral-bacterial AII aetiology.
EFFECT: more accurate diagnosing of the aetiology of acute intestinal infection and simplifying the diagnostic procedure.
SUBSTANCE: polymerase chain reaction method is used to recognise polymorphous variants of IL6 and TGFb1 genes. Recognising the homozygous genotype CC in -174 position of IL6 gene in males and females, as well as the heterozygous genotype GC in -915 position of TGFb1 gene in females enables predicting the high risk of the complicated clinical course of the urogenital Chlamydial infection.
EFFECT: invention enables deciding on reasonable grounds on selecting a therapeutic approach to a specific patient suffering from urogenital Chlamydial infection in order to prevent complications of the urogenital Chlamydial infection and reproductive dysfunctions.
4 tbl, 5 ex
SUBSTANCE: invention concerns diagnosing undifferentiated connective tissue dysplasia (UCTD) in females with a personal history of miscarriage. The technique involves determining the fibrinolytic activity in an endometrial biopsy sample. If the value is less than 23.55 mm2, undifferentiated connective tissue dysplasia is diagnosed.
EFFECT: invention provides the high diagnostic accuracy and enables diagnosing UCTD in the females with the personal history of early miscarriage.
2 tbl, 2 ex
SUBSTANCE: instrument comprises an oral sample collection vessel, a detector able to detect a marker in this sample, an indicator actuated by a detector signal. The above vessel is detachably connected to an oral cavity instrument. The vessel comprises a sample collection element, a sample storage container, and a passage connecting the collection element and the container to supply the sample to the container by capillary action. The indicator is integrated into the container. The declared instrument is used to diagnose oral diseases by collecting the oral sample, detecting one or more markers in this sample and indicating the presence of one of the disease markers.
EFFECT: inventions enables establishing an accurate and fast diagnosis of the oral pathologies accompanying the daily oral care by placing the detector inside the container able to accumulate a required amount of the sample to be diagnosed.
25 cl, 1 dwg
SUBSTANCE: technique involves the clinical-laboratory examination of a sportsman who completed heavy physical activity 12-16 hours ago. The examination extent is determined taking into account the organs and systems most vulnerable to the physical activity while deriving the prognostically significant criteria of the morphofunctional body state. The examination involves measuring and analyzing the biochemical, haematological, immunological and functional values, as well as vitamin-mineral saturation. And if the above values are stably unchanged, reliably different from the norm, nonspecific changes of the sportsman's organs and systems are diagnosed.
EFFECT: technique provides the early diagnosis of the significant changes of the organs and systems during trainings and competitions that enables taking further timely measures to prevent the further progression of pathological conditions and maintaining thereby occupational performance and achieving stable high sport results.
SUBSTANCE: efficiency of treatment of patients with high grade non-Hodgkin malignant lymphoma is determined by the likelihood of achieving remission, and 5-year total and relapse-free survival. The method comprises the study of polymorphism G13494A 6th intron of gene TP53 of the patient. In case of revealing in the patient of homozygous genotype G/G in a given locus the low efficiency of treatment is predicted, namely, low likelihood of 5-year survival of the patient and low likelihood of absence of relapse. In the case of revealing in the patient of the genotype A/A or G/A in a given locus, the high efficiency of treatment is predicted, namely the high likelihood of remission and 5-year survival of patient.
EFFECT: invention enables to assess the efficiency of treatment of patients with high grade non-Hodgkin malignant lymphoma on the degree of polymorphism G13494A of 6th intron of gene TP53.
5 dwg, 10 tbl, 2 ex
SUBSTANCE: invention represents a diagnostic technique for the disturbed thrombocyte aggregation accompanying mucoviscidosis in children involving a thrombocyte aggregation test using the Multiplate aggregometer inducers. Trays with a magnetic mixer and electrodes are added with NaCl 400 mcl at 37°C and immediately added with whole blood 400 mcl from a hirudin test tube, incubated in the chamber for two minutes; the tray is added with 30 mcl of an aggregation inducer specified in a group: soluble thrombin receptor - peptid-6, adenosine diphosphate, arachidonic acid. The thrombocyte aggregation rate is displayed on the screen in the form of a curve, and the sub-curve area U is automatically calculated; the sub-curve area U shows the thrombocyte aggregation state as compared to reference values in the group of healthy children; if the threshold area U has appeared to exceed the reference, the thrombocyte hyperaggregation, while the threshold area U being less than the reference, the thrombocyte hypoaggregation is stated.
EFFECT: invention provides the timely diagnosis of microcirculatory disorders accompanying mucoviscidosis.
2 ex, 1 dwg
SUBSTANCE: invention relates to the field of microbiology, namely to a method of microorganism characteristic. The essence of the method consists in the following: (a) obtaining a sample to be tested, about which it is known that it contains or can contain microorganisms; (b) layering the tested sample on a density buffer in a container, where the said density buffer possesses a uniform density from approximately 1.025 to approximately 1.120 g/ml; (c) addition of an identifier into the said tested sample and/or into the said density buffer; (d) centrifugation of the said container in order to separate microorganisms from other components of the said tested sample and to form a deposit of microorganisms; (e) spectroscopic analysis of the deposit and/or the said one or more than one identifier with obtaining measurements, which characterise the microorganisms, where the said spectroscopic analysis is carried out when the said deposit is located in the said container; and (f) characteristic of the microorganisms in the deposit on the basis of the obtained measurements and/or the presence or absence of the said identifier or a metabolised form of the said identifier in the deposit, where the said microorganisms are characterised by one or more classification models, selected from the group, consisting of Gram groups, clinical Gram groups, therapeutic and functional groups.
EFFECT: application of the claimed invention makes it possible to increase the accuracy of the microorganism characteristic.
15 cl, 5 dwg, 1 tbl, 4 ex
SUBSTANCE: invention refers to medicine, namely to qualitative differential instant diagnostic technique for benign and malignant periglottis new growths as shown by oral fluid biomarkers. Substance of the method consists in measuring a quantity of matrix metalloproteinase 2 (MMP 2) in patient's oral fluid; the clinical reference is the level of 1.7-2.9 ng/ml; if the MMP 2 content is 14.4-24.3 ng/ml, patient's periglottis papilloma is diagnosed; if the patient's oral fluid MMP 2 content is 4.1-6.8 ng/ml, periglottis cancer is diagnosed. A biomarker for the qualitative differential instant diagnosis of the periglottis new growths is a tissue inhibitor of metalloproteinase 2 (TIMP 2); the clinical reference is a level of 6.44-11.23 ng/ml; if the TIMP 2 content 29.25-48.75 ng/ml, patient's periglottis papilloma is diagnosed; the TIMP 2 content being 57.23-95.03 ng/ml, periglottis cancer is diagnosed.
EFFECT: using the declared technique enables providing more accurate differential diagnosis of the benign and malignant periglottis new growths.
2 cl, 6 ex
FIELD: medicine, hepatology.
SUBSTANCE: one should detect the level of hepato-specific enzymes (HSE) in blood plasma, such as: urokinase (UK), histidase (HIS), fructose-1-phosphataldolase (F-1-P), serine dehydratase (L-SD), threonine dehydratase (L-TD) and products of lipid peroxidation (LP), such as: dienic conjugates (DC), malonic dialdehyde (MDA). Moreover, one should detect the state of inspecific immunity parameters, such as: immunoregulatory index (IRI) as the ratio of T-helpers and T-suppressors, circulating immune complexes (CIC). Additionally, one should evaluate the state of regional circulation by applying rheohepatography (RHG), the system of microhemocirculation with the help of conjunctival biomicroscopy (CB) to detect intravascular index (II). In case of increased UK, HIS levels up to 0.5 mcM/ml/h, F-1-P, L-SD, L-Td, LP products, CIC by 1.5 times, higher IRI up to 2 at the norm being 1.0-1.5, altered values of regional circulation, increased II up to 2 points at the norm being 1 point, not more one should diagnose light degree of process flow. At increased level of UK, HIS up to 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 1.5-2 times, increased IRI up to 2.5, altered values of regional circulation, increased II up to 3-4 points one should diagnose average degree of process flow. At increased level of UK, HIS being above 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 2 and more times, increased IRI being above 2.5, altered values of regional circulation, increased II up to 5 points and more one should diagnose severe degree of process flow.
EFFECT: higher accuracy of diagnostics.
FIELD: medicine, infectology, hepatology.
SUBSTANCE: in hepatic bioptate one should detect products of lipid peroxidation (LP), such as: dienic conjugates (DC), activity of antioxidant enzymes, such as: catalase (CAT)and superoxide dismutase (SOD). One should calculate by the following formula: C = DC/(SOD x CAT)x100, where DC - the content of dienic conjugates, SOD - activity of superoxide dismutase, CAT - activity of catalase. At coefficient (C) values being above 65 one should predict high possibility for appearance of cirrhosis, at 46-645 - moderate possibility and at 14-45 -low possibility for appearance of cirrhosis.
EFFECT: higher accuracy of prediction.
FIELD: medicine, clinical toxicology.
SUBSTANCE: at patient's hospitalization one should gather the data of clinical and laboratory values: on the type of chemical substance, patient's age, data of clinical survey and laboratory values: body temperature, the presence or absence of dysphonia, oliguria being below 30 ml/h, hemoglobinuria, erythrocytic hemolysis, exotoxic shock, glucose level in blood, fibrinogen and creatinine concentration in blood serum, general bilirubin, prothrombin index (PTI), Ph-plasma, the state of blood clotting system. The state of every sign should be evaluated in points to be then summed up and at exceeding the sum of points being above "+20" one should predict unfavorable result. At the sum of "-13" prediction should be stated upon as favorable and at "-13" up to "+20" - prediction is considered to be doubtful.
EFFECT: higher accuracy of prediction.
2 ex, 3 tbl
FIELD: medicine, juvenile clinical nephrology.
SUBSTANCE: disease duration in case of obstructive pyelonephritis should be detected by two ways: either by detecting the value of NADPH-diaphorase activity, as the marker of nitroxide synthase activity in different renal department and comparing it to established norm, or by detecting clinico-laboratory values, such as: hemoglobin, leukocytes, eosinophils, urea, beta-lipoproteides, lymphocytes, neutrophils, the level of glomerular filtration, that of canalicular reabsorption, urinary specific weight, daily excretion of oxalates, arterial pressure, and estimating their deviation against average statistical values by taking into account a child's age.
EFFECT: higher efficiency of detection.
7 dwg, 1 ex, 6 tbl
FIELD: clinical medicine, pulmonology.
SUBSTANCE: one should carry out complex estimation of interleukin-1β) concentration in blood, saliva, bronchoalveolar liquid. Moreover, one should detect distribution coefficient (DC) for IL-1β as the ratio of IL-1β blood content to IL-1β salivary content. At increased IL-1β blood content by 10 times and more, by 2 times in saliva, unchanged level of bronchoalveolar IL-1β, at DC for IL-1β being above 1.0 one should predict bronchial obstruction. The method enables to conduct diagnostics of the above-mentioned disease at its earlier stages.
EFFECT: higher efficiency of prediction.
FIELD: medicine, diagnostics.
SUBSTANCE: the present innovation deals with genetic trials, with diagnostic field of oncological diseases due to analyzing DNA by altered status of gene methylation that take part in intracellular regulation of division, differentiating, apoptosis and detoxication processes. One should measure the status of methylation in three genes: p16, E-cadherine and GSTP1 in any human biological samples taken out of blood plasma, urine, lymph nodes, tumor tissue, inter-tissue liquid, ascitic liquid, blood cells and buccal epithelium and other; one should analyze DNA in which modified genes of tumor origin or their components are present that contain defective genes, moreover, analysis should be performed due to extracting and purifying DNA out of biological samples followed by bisulfite treatment of this DNA for modifying unprotected cytosine foundations at keeping 5-methyl cytosine being a protected cytosine foundation followed by PCR assay of bisulfite-treated and bisulfite-untreated genes under investigation and at detecting alterations obtained according to electrophoretic result of PCR amplificates, due to detecting the difference in the number and electrophoretic mobility of corresponding fractions at comparing with control methylated and unmethylated samples containing normal and hypermethylated forms of genes one should diagnose oncological diseases. The method provides higher reliability in detecting tumors, detection of remained tumor cells after operation.
EFFECT: higher efficiency of therapy.
1 cl, 3 dwg, 4 ex
FIELD: medicine, gastroenterology.
SUBSTANCE: one should carry out diagnostic studying, moreover, on the 5th -6th d against the onset of exacerbation in case of gastric and duodenal ulcerous disease one should detect the content serotonin, histamine and acetylcholine in blood, then during 2-3 wk one should conduct medicinal therapy to detect serotonin, histamine and acetylcholine level in blood again and at serotonin content being by 2-3 times above the norm, histamine - by 1.15-1.4 times above the norm and acetylcholine - by 20-45% being below the norm one should predict the flow of gastric and duodenal ulcerous disease as a non-scarring ulcer.
EFFECT: higher accuracy of prediction.
SUBSTANCE: method involves taking blood from ulnar vein (systemic blood circulation) and from large vein of the injured extremity proximal with respect to lesion focus (regional blood circulation). Spontaneous NST-test value is determined and difference is calculated in systemic and regional blood circulation as regional-to-systemic difference. The difference value is used for predicting clinical course of pyo-inflammatory disease in extremities.
EFFECT: high accuracy of diagnosis.
4 cl, 2 tbl
FIELD: medicine, gastroenterology.
SUBSTANCE: one should introduce biologically active substance, moreover, in patient's blood serum one should detect the content of acetyl choline and choline esterase activity followed by 2-h-long intragastric pH-metry at loading with biologically active substance as warm 40-45%-honey water solution at 35-40 C, and at increased content of acetyl choline being above 1.0 mM/l, choline esterase being above 0.5 mM/l/30 min and pH level being 6.0-6.9 it is possible to consider apitherapy to be useful for treating ulcerous duodenal disease.
EFFECT: higher efficiency and accuracy of detection.
FIELD: medicine, gastroenterology.
SUBSTANCE: it has been suggested a new method to detect pharmacological sensitivity to preparations as acidosuppressors. After the intake of the preparation a patient should undergo fibrogastroduodenoscopy 3 h later, then, through endoscopic catheter one should introduce 0.3%-Congo red solution intragastrically and the test is considered to be positive at keeping red color that indicates good sensitivity to the given preparation, and in case of dark-blue or black color the test is considered to be negative that indicates resistance to this preparation. The suggested innovation widens the number of diagnostic techniques of mentioned indication.
EFFECT: higher efficiency of diagnostics.