Diagnostic technique for renal functional reserve |
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IPC classes for russian patent Diagnostic technique for renal functional reserve (RU 2528903):
Diagnostic technique for clinically latent hypercorticoidism in patients suffering from stype 2 diabetes mellitus or obesity / 2521387
First stage comprises a night suppressive test with dexamethasone 1 mg with a test considered to be positive, if plasma cortisol measured at 8.00 in the next morning exceeds 50 nmole/l. If the first stage has a positive result, the second stage is performed 1-2 days later. At the second stage, blood plasma cortisol at 24.00, daily urine free cortisol, a coefficient of circadian rhythm of cortisol secretion are determined on the same day. If at least two of the three test results are above normal: plasma cortisol at 24.00 is more than 207 nmole/l, daily urine free cortisol is more than 180 mcg/day, coefficient of circadian rhythm of cortisol secretion is more than 50%, hypercorticoidism syndrome is diagnosed. The presented technique provides higher accuracy and simplifies diagnosing of the given disease.
 Solid phase enzyme-immunoassay (eliza) for vascular endothelial growth factor (vegf) / 2517301
Invention relates to the field of immunology, namely to enzyme-immunoassay, in particular to a method of detecting forms of vascular endothelial growth factor (VEGF) with a size more than 110 amino acids in a biological sample. The method includes the following stages: contact and incubation of the biological sample with an uptake reagent, immobilised on a solid substrate, where the uptake reagent contains a monoclonal antibody, which recognises and specifically binds with residues, in quantity more than 110, from human VEGF; separation of the biological sample from the immobilised uptake reagents; contact of the immobilised molecular complex of the reagent of the uptake-target with detected antibody, which binds with VEGF domains, responsible for binding with KDR and/or FLT1 receptor, or which binds with an epitope in VEGF1-110; measurement of the level of VEGF110+, bound with reagents of the uptake, with application of means of detection for the detected antibody. Set of immune assay reagents for detection of VEGF110+ forms in the biological sample. An antibody 5C3, obtained from hybridoma 5C3.1.1 with a depositary number PTA-7737, with the said antibody 5C3 binding VEGF110+ forms, including VEGF121+. Hybridoma 5C3.1.1, deposited in ATCC with the depositary number PTA-7737, to obtain the monoclonal antibody 5C3.
Method for determining degree of severity of chronic obstructive pulmonary disease / 2516971
Blood is examined. The steroid hormones cortisol (nmole/l) and DHEA-S (mcmole/l), as well as the oxidative stress values - oxidative modified proteins (OMP, nM/mg, protein), malondialdehyde (MDA, nM/ml) and SH groups mg% are evaluated. The forced expiratory volume 1(%) FEV1 is calculated by formula on the basis of the derived values. If the derived FEV1 is within 50% to 80%, the presence of a moderate degree of chronic obstructive pulmonary disease; the FEV1 being within the range of 30 to 49% means a severe degree of chronic obstructive pulmonary disease (COPD), and the derived value being less than 30% shows an extremely severe degree of COPD.
Method for prediction of risk of early development of atherosclerosis in patients with chronic prostatitis / 2504782
Blood serum of the younger patient suffering chronic prostatitis is examined for total testosterone, sex hormone-binding globulin to calculate a free testosterone index; high-density lipoproteins and triacylglycerides are determined, and an atherogenic index is calculated by formula. If the atherogenic index is <3.7, a high risk of the early development of atherosclerosis is predicted.
 Method for prediction of recurrent non-small-cell lung cancer / 2498305
What is involves is the histological examination of tissue fragments taken from the extracted lung with primary, intermediate and segmental bronchi at 4-5 cm from the tumour, and disregeneration change cases are determined in the respiratory bronchial epithelium, including: basal cell hyperplasia (BCH), squamous metaplasia (SM) and if observing a combination of basal cell hyperplasia and squamous metaplasia (BCH+SM+) in the respiratory bronchial epithelium adjacent to the tumour, a risk of developing the recurrent non-small-cell lung cancer is predicted.
 Method for prediction of recurrent cervical cancer / 2485523
Method for prediction of recurrent cervical cancer involves biochemical daily urine analysis to determine daily urine androsterone and etiocholanolone to be related; if the relation is 0.75 mg/day or less, the recurrent disease is predicted for the first 2 years, and if the relation exceeding 0.75 mg/day, a prolonged recurrence-free period up to 10 years or more is predicted.
 Method for aromatase activity test / 2481587
Blood serum is examined for lutropin, follitropin, oestradiol, total testosterone and free testosterone levels before and 48 hours after the oral administration of 450 aromatase inhibitor, letrozole 10 mg, and the variations of lutropin and follitropin, as well as of the oestradiol/total testosterone and oestradiol/free testosterone relations are used to asses aromatase activity in points assigned by a certain way to consider low aromatase activity shown by total score 0 to 7 points, normal aromatase activity - 8 to 14 points, and high aromatase activity - 15 points or more.
Method for specifying directionality of pathological process in mucinous gastric carcinoma / 2480771
In males with mucinous gastric carcinoma after a curative resection to the extent of gastrectomy, tumor tissue is analysed for the level of testosterone. If the tissue value is 7.8±0.6 ng/g, process generalisation within 6 to 12 months is predicted; the level of tissue testosterone 174.5±15.6 ng/g enables predicting the favourable clinical course of the process, prolongation of the patient's life.
Method for prediction of recurrent endometrial polyps in postmenopausal women / 2478959
Transvaginal echography in the patients suffering endometrial polyps is conducted to measure an M-echo thickness, mm, the presence of obesity (Ob), blood follicle-stimulating hormone (FSH), IU/l, oestradiol (E2), pg/l; an insulin-resistance index is calculated by HOMA technique; a therapeutic strategy (ThStra) is taken into consideration; a probability of developing recurrent endometrial polyps (p) 1 year after the operation is determined by formula. If the probability exceeds 0.7, a high risk of recurrence is predicted. The probability falling within the range of 0.5 to 0.7 shows a moderate risk, while the probability of 0 to 0.5 shows a low risk.
Diagnostic technique for extraintestinal manifestations of celiac disease / 2476892
Blood serum is examined for prolactin and folic acid, and if the prolactin level is 30.6 ng/ml and more, while the folic acid level is 8.7 ng/ml and lower, the extraintestinal manifestations of celiac disease are diagnosed.
 Method for quantitative measurement of carbon nanostructures in biological samples and body distribution thereof / 2528096
Carbon nanostructure surface is modified with (2,4,5-triiodophenyl)-methanol; the modified carbon nanostructures are analysed to measure a iodine amount; the prepared modified carbon nanostructures are administered into an experimental animal's body to take organs and tissues thereafter, to homogenise them in a 0.5-2 M NaOH solution, to sample a homogenate, to dilute with water, to expose the diluted sample to ultrasound to a temperature of 40-70°C, to determine a iodine amount in the prepared sample by mass spectrometry with inductively bound plasma and to calculate the carbon nanostructure content in the sample as shown by a difference of the iodine amount in the sample prior to administration of the modified carbon nanostructures and after administration thereof into the body and to re-calculate this iodine amount into the carbon nanostructure content in the sample with the use of the initial iodine content in the modified carbon nanostructure.
Method for prediction of clinical effectiveness of patients with non-hodgkin lymphomas with bone marrow involvement / 2526796
Invention refers to medicine, namely to oncohaematology, and can be used for prediction of the clinical effectiveness in the patients with non-Hodgkin lymphomas with bone marrow involvement. That is ensured by determining a lymphocyte volume and electric conductivity in patient's peripheral blood before the treatment and every next course of the chemotherapy with calculating a relation of the values. If observing a decrease of the relations as compared to the initial value, the positive clinical effectiveness and the absence of generalisation symptoms are predicted. The absence of the decrease or increase of the relations shows the absence or low clinical effectiveness and the presence of the symptoms of a continued tumour growth.
Method of differential morphometric diagnostics of erythrodermal form of mycosis fungoides and syndrome of skin pseudolymphoma by relative volume of epidermis and mitotic index of epidermal cells / 2526180
To perform differential morphometric diagnostics of an erythrodermal form of mycosis fungoides and the syndrome of skin pseudolymphom performed are: histological analysis of the affected skin biopsy samples by a method of light microscopy, immunodifferentiation of infiltrate cells, additional morphometric analysis of basal keratinocytes in histological preparations of the affected skin biopsy samples. In case of the similar histological picture, described in case of erythrodermal form of mycosis fungoides and the syndrome of skin pseudolymphoma such as acanthosis, hyperkeratosis, parakeratosis, oedema and vasodilatation of the derma, lymphohistiocytic infiltrates, located in the upper third of the derma, similar immunophenotype of the infiltrate cells in case of erythrodermal form of mycosis fungoides and the syndrome of skin pseudolymphoma, represented by CD2+, CD3+, CD4+, CD5+, CD43+, CD45RO+ lymphocytes, morphometric analysis of all epidermis cells is performed. After that, the relative volume of epidermis and mitotic index of the epidermal cells are calculated. In case of increase of the relative volume of the epidermis by 2.2 and more times in comparison with the normal skin and an increase of the mitotic index of the epidermal cells by 2.7 times and more in comparison with the normal skin, erythrodermal form of mycosis fungoides is diagnosed, and in case of the unchanged relative volume of the epidermis and the mitotic index of the epidermal cells in comparison with the normal skin, the syndrome of skin pseudolymphoma is diagnosed.
 Method of controlling biochemical reactions / 2525439
Invention relates to biochemistry and can be used to control biochemical reactions in vitro and in vivo. Control is carried out by exposing a magnetic nanosuspension containing a bioactive macromolecule, attached directly or through a ligand to single-domain magnetic nanoparticles, to an external low-intensity low-frequency alternating magnetic field which provides deformation and/or change in conformation of bioactive macromolecules participating in the reaction.
Diagnostic technique for arteriovenous fistula stenosis in long-term hemodialysis patients with end-stage chronic renal disease / 2522397
Arteriovenous fistula stenosis in long-term hemodialysis patients with end-stage chronic renal disease is diagnosed by four or more criteria: mean corpuscular volume (MCV) more than 93.3 ml, mean corpuscular hemoglobin (MCH) of more than 32.2 pg/l, urea concentration (Urea) more than 15.9 mmole/l, post-dialysis urea (Ur_KT/V) more than 4.2 mmole/l, mid-dialysis urea (Ur_PCR) more than 4.5 mmole/l, as well as dialysis efficacy (Kt/V) less than 1.71 units and equilibrated Kt/V (Kt/Veqv) less than 1.54 units.
 Method for prediction of developing bacterial complications with underlying acute respiratory viral infection / 2522202
Invention refers to medicine, and can be used for the prediction of developing bacterial infections in the patients suffering acute respiratory viral infection prior to clinical presentations. Substance of the method: NAPD-dependent glutamate dehydrogenase activity in peripheral blood lymphocytes of the patient suffering acute viral infection is measured. If the value is more than 2.89 mcU/10000 cells, the bacterial complications are predicted to develop within 3-5 days after admission to hospital.
 Method for determining penetration depth of light into skin and device for implementing it / 2521838
Group of inventions refers to medical instrument engineering. Skin and a calibration sample are exposed to optical radiation in at least Nλ≥3 narrow or wide spectral regions Λk (k=1,…,N). Signals emitted by the skin and calibration sample are recorded with an activated and de-activated radiation emitter. Diffusion reflection factors R(Λk) are derived from the relation
 Method and device for cell sorting / 2520848
Invention relates to biotechnology and represents a device and a system for identification and selective change of a required subpopulation of cells in a population with cell samples. The device and system include a path of liquid movement. The device and system include application of an objective, an optic axis of which is located coaxially to the path of jet movement in a focal point. The device and system include a detector for detection of light, focused by the objective, a logical programme, interfaced with the detector, used to determine whether a cell in the population with the cell samples is a part of required subpopulation of cells, and to output signals basing on determination whether cell is part of the required subpopulation of cells, and a controlled power source, interfaced with the logical programme, used for selective change of either cells in the required subpopulation of cells or the cells, which do not belong to the required subpopulation of cells, in accordance with a signal sent by the logical programme.
 Method for activation of white-cell-rich suspension growth and complex correction of blood composition in acoustic field in vitro / 2518534
Blood or its fractions are exposed to broadband oscillating acoustic signals at a temperature of 4°C to 38°C within the frequency range of 16-20000 Hz with a dominating level of noise pressure more than 45 dB, and less than 60 dB at 60-80 beats per minute, or to sequentially alternating acoustic signals with the dominating level of noise pressure ≤45 dB at <60 beats per minute and acoustic signals with the dominating level of noise pressure more than 65 dB, and less than 90 dB at >80 beats per minute.
 Method for determining optical and biophysical tissue parameters / 2510506
Radiation is supplied onto a tissue into one or more points at wavelengths λ from the range of 350-1600 nm; a diffuse reflection P(L, λ) is measured at wavelengths of the supplied radiations for each lighting point; an absolute R(L, λ) or normalised r(L, λ) spectral-spatial profile of a tissue diffuse reflection coefficient, while the optical and biophysical parameters (X) are determined on the basis of analytic expressions representing multiple regressions between X and R(L, λ) or between X and r(L, λ) produced by measuring or calculating by Monte-Carlo method R(L, λ), r(L, λ) for a number of biotissue samples or control phantoms with the known optical and biophysical parameters; an implementing ensemble of the optical and biophysical parameters of the biotissue and respective spectral-spatial profiles R(L, λ), r(L, λ) for the ranges of the tissue optical and biophysical parameters.
 Express-diagnosis method for determining blood erythrocytes condition in fetus and newborns suffering from hemolytic disease / 2249213
Method involves examining blood erythrocytes. Blood erythrocyte suspension fluorescence intensity is measured at wavelength of 500 nm polarized along light axis and then at wavelength of 493 nm in perpendicular to the light axis for each polarization direction with following anisotropy value being calculated. Its deviation from norm proves availability of hemolytic disease.
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FIELD: medicine. SUBSTANCE: invention refers to medicine. Substance of the early diagnostic technique for chronic renal disease consists in using a dosage range of dopamine of 1 to 3 mcg/kg of body weight and a standard water load of 200 ml. No glomerular filtration rate increase testifies to the presence of early signs of chronic renal disease. EFFECT: using the declared technique enables standardising the examination as high as possible by precise dosage measurement of the preparation. 2 tbl, 2 ex
The invention relates to medicine, namely to Nephrology, and can be used in Nephrology for the early diagnosis of hidden impaired renal function. Many animal studies and limited human studies with great confidence suggest that the progression of chronic kidney disease (CKD) is largely due to metabolic and hemodynamic factors, to a lesser extent the activity of the underlying disease. Identification of these factors, such as vnutriklubnoy hypertension and glomerular hypertrophy, very important, as there are possibilities of their correction. Thus, vnutriklubnoy hypertension underlies the progression of CKD; its identification is based on the definition of hyperfiltration and/or reduction of functional renal reserve (FPR) (G.P. Arutyunov the Problem of hyperfiltration in clinical practice / Gperatlon, Lgorbunova // Clinical Nephrology. - 2009. No. 1. - P.29-40). The first method of determining the lateral distribution suggested J.P. Bosch (Bosch JP, Saccagi A., LauerA. Renal Functional reserve in humans: Effect of protein intake on glomerular filnation rate. Am.J.Med. - 1983, v.75, p.943-950); still most often used methods of diagnosis DPF using load meat protein inside or protein solutions. Some researchers have used intravenous protein or solutions aminos the slot, this improves the accuracy of the method due to its standardization, at the same time significantly increasing its cost, used amino acid solutions also differ in composition (Kucher A.G. Features functional response of the kidneys healthy people on loads of different types of protein and its derivatives / Aguer, Amecan, Waikoloan [and other] // Nephrology. - 1999. - V.3, №4. - C-90). The closest analogue of the method is a method of diagnosing a DPF using a load of meat protein (patent No. 2308725 (RF) IPC G01N 033/70 "Method of determination of functional renal reserve"), which determine the difference in glomerular filtration rate in the clearance of endogenous creatinine between protein-free breakdown and breakdown with meat load, and the collection of urine is produced between the second and sixth hour after a protein-free lunch and the next day in the same period after cold stress (5 g of meat per kg of body weight). The use of meat products obviously leads to inaccurate results, as meat birds and animals differ in composition and quantity of the protein; in addition, it leads to certain disadvantages in the preparation of samples in practice. One drawback of the sample is also the duration of the procedure - it takes almost two days. The technical result is achieved due to the fact that the method for the diagnosis of lateral distribution on the denotes the sample with a load of Dopamine to increase glomerular filtration rate, in the absence of a rise in glomerular filtration rate compared with the basal value diagnose early signs of chronic kidney disease. Dopamine - synthetic analogue of Dopamine, a vasoactive agent, according to the chemical structure related to the catecholamines (the precursor of norepinephrine), has the ability to in small doses to increase vnutripochechnykh the blood flow and glomerular filtration. The absence of such an increase indicates a depletion of the reserve capacity of the kidney result in damage to the nephrons, which allows you to diagnose early preclinical stage of CKD. The proposed method is as follows. After emptying the bladder of the patient in the morning on an empty stomach (medication cancelled) drink 200 ml of water to ensure the normal speed of diuresis, then within one hour collects urine by free urination. At the end of the hour the patient takes a blood sample (5 ml), which defines the level of endogenous creatinine. Next is intravenous drip infusion of Dopamine at a dose of 1-3 mcg/kg/min for 30 minutes (0.05 to 0.1 ml of 4%aqueous solution of Dopamine, diluted with 200 ml of 0.9%Sodium Chloride), after which the patient again within hours collects urine. In both urine samples is determined by the amount of creatinine (CR). Further defines who I glomerular filtration rate (GFR) before and after the load according to the formula of calculation of creatinine clearance with bringing to the surface area of the body: (SG urine (mmol/l) × Minute diuresis)/IG of blood (mmol/l). Functional renal reserve calculated according to the formula: (SKF-SKF)/100. The NRF is regarded as safe when values of more than 10%, underweight - less than 10%. Clinical tests of the method have shown the feasibility of its use in therapeutic and Nephrology clinic. We studied the accuracy of the proposed method in healthy volunteers and in different clinical groups of patients (table 1) (in brackets the 95% confidence interval, where ** p<0,01).
Table 1 presents the results of a survey of 41 healthy person (no signs of kidney disease, hypertension, diabetes) and 20 patients with what ryznicami CKD, that is, bladder syndrome, high creatinine level and glomerular filtration rate less than 60 ml/min/1.73 m2. The results show that healthy we are seeing positive DPF, while in patients it is negative. To assess the NRF previously used doses of Dopamine from 1 to 3 mcg/kg/min in order to choose the best dose, 9 healthy volunteers test was performed twice with an interval of 8-10 weeks, respectively, with a dose of 3 mcg/kg/min and 1 mcg/kg/min All other conditions of the test were unchanged. The results revealed no differences: DPF ranged 19,8 [13,2; 31,6]% at the dose of 1 mcg/kg/min and reached 29,2 [20,9;46,4]% at the dose of 3 mcg/kg/min (p>0,05). The action method can illustrate the results of the study and observation of two patients with systemic lupus erythematosus (SLE). Patient S., 24, sore SLE 12 years. The disease made its debut lesions of the skin and joints, signs of nephritis in the form of bladder syndrome first discovered 1 year ago. At the time of the surveys (2006) have high (III) the degree of activity of the disease, the symptoms of nephrotic form of secondary glomerulonephritis, hypertension, grade II CKD stage 1. Patient F., 27 years old, is ill SLE 4 years. The disease made its debut lesions of the skin and mucous membranes, signs porage the Oia kidney during the year. At the time of the study (2006) identified the characteristics of high (III degree) activity, damage to the kidneys in the form of nephritic form of secondary glomerulonephritis, hypertension, grade II CKD 1. Both patients was conducted as a complete clinical examination and evaluation of the lateral distribution in the acute test with Dopamine at a dose of 1 mcg/min Thus, we have two cases of SLE in young patients with high disease activity and active lupus-nephritis, with normal renal function, mild proteinuria, but with a completely different evaluation results DPF: negative results in S. and positive in F. (see table 2).
Against the backdrop of ongoing immunosuppressive therapy, the patient's condition has stabilized, in both cases, remission of the disease, and glomerulonephritis in the form of reduction of proteinuria less than 500 mg per day, the complete disappearance of bladder syndrome in both cases was not achieved. However, the onset of the exacerbation requested a biopsy. The biopsy results, the patient With. in 2008, showed signs of significant as glomerulosclerosis, nephrosclerosis, despite continuous therapy GFR in 2009 reached 48 ml/min/1.73 m2then there was the progression of CKD. At the same time, despite the third for 6 years aggravation of the patient F. the results of the biopsy showed only signs of active lupus nephritis IV morphological class with minimal signs of sclerosis. The above typical clinical examples illustrate the accuracy of the test in determining irreversible hemodynamic disturbances. The inventive method allows to standardize the study due to the accurate dosing of the drug, eliminates the influence of such factors as the quality and composition of meat, organs of digestion, the absorption of the protein, in addition, is affordable, simple and the one for the doctor and patient study. The method of early diagnosis of chronic kidney disease using a sample with a load of Dopamine, characterized in that use a range of doses of Dopamine from 1 to 3 µg/kg body weight and standard water load in the amount of 200 ml and the absence of a rise in glomerular filtration rate is judged on the early signs of chronic kidney disease.
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