Method for prediction of recurrent endometrial polyps in postmenopausal women |
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IPC classes for russian patent Method for prediction of recurrent endometrial polyps in postmenopausal women (RU 2478959):
Diagnostic technique for extraintestinal manifestations of celiac disease / 2476892
Blood serum is examined for prolactin and folic acid, and if the prolactin level is 30.6 ng/ml and more, while the folic acid level is 8.7 ng/ml and lower, the extraintestinal manifestations of celiac disease are diagnosed.
 Method for steroid profiling in dope test of sportsmen / 2467331
Invention refers to medicine, namely sports medicine, and may be used for steroid profiling in dope test of sportsmen. That is ensured by urine sample hydrolysis by a mixture of two enzymes: β-glucuronidase E.coli and arylsulphatase H.pomatia in volume ratio 1:1 to 1:3 in the presence of a citrated buffer solution and an internal standard. The hydrolysate is separated, derivatised that is followed by chromatic/mass-spectral analysis of the sample and recording of the derived results and stating the presence of endogenic steroids (ES). The ES value is determined in a sportsman for at least 10-15 times every 10-12 days. The analysis results are used to set minimum and maximum values of each of the derived ES. Said values are accepted to be a confidence range, and a steroid profile of the sportsman is drawn as a set of confidence ranges of all found ES.
Method of determining clinical effectiveness in cervical cancer / 2464576
One week after the termination of polychemotherapy, daily urine is examined for the amount of cortisol and cortisone, while blood is examined for adrenocorticotropic hormone. It is followed by calculating the relation of total cortisol and cortisone to adrenocorticotropic hormone. If the derived value is 0.74 and higher, high responsiveness to chemopreparations is stated.
Method for describing nature of arrested sexual development in boys in puberty with growth retardation / 2462720
Examination results are used to detect boys with the absence or insufficiency of blood concentrations of at least two of three hormones (luteinising hormone, follicle-stimulating hormone, total testosterone) to be used for the clinical course of transcranial magnetic therapy. The concentration of all hormones are evaluated in 1-1.5 months, and if observing increase of the concentrations of at least two hormones, including testosterone by min. 25% from the initial values, a functional nature of arrested sexual development is stated. If observing no increase or increase of the concentrations of two hormones, including testosterone by max. 25% from the initial values, an organic nature of arrested sexual development is stated.
 Diagnostic technique for idiopathic nasal breathing irregularities in pregnant women / 2456604
Blood serum of a pregnant woman is examined for the oestradiol concentration by enzyme-linked immunosorbent assay. Observing the oestradiol concentrations exceeding normal values according to weeks of gestation in 1.4 times and more enables stating idiopathic nasal breathing irregularities accompanying a period of pregnancy.
Method for prediction of level of efficiency of reduction of manifestation severity of atopic dermatitis / 2453851
Pre-therapy blood plasma is analysed for immunoglobulin E, adrenocorticotrophin and endorphin, the index SCORAD dynamics is determined by formula: Dsk=1.34147-0.000820741*IgE-0.00382642*ACTP+0.0209401*endorphin wherein IgE is the level of acute blood plasma immunoglobulin E mIU/ml; ACTP is the level of acute blood plasma adrenocorticotrophin ng/l. If observing the Dsk value exceeding 1.18, a prognosis of improving clinical dynamics of recovery is favourable; the conventional therapy is considered to be sufficient. The Dsk value being equal to 1.18 or less, the improving clinical dynamics of recovery is unfavourable, the intensified therapeutic scheme for atopic dermatitis is considered to be preferential.
 Method for identification and classification of 3-oxosteroids and their metabolites in doping test of sportsmen / 2452967
Blood plasma is examined for the presence and the position of double bonds and the related differences in structural characteristics. Common and examined steroids are chemically modified by a carboxyl group (oximes); their mass spectra are read out and recorded; characteristic ions (lc) and neutral loss (Do) are specified in the mass spectra. The specified lc and Do values are related to those specific for common 3-oxosteroid oximes by the absolute value. Herewith it is only the similar lc and Do values from both sides differing by 0.3% and less that are taken into consideration; the comparison results are used to identify 3-oxosteroid oximes.
Method of antenatal prediction of consequences of perinatal lesions of nervous system in children / 2449287
Clinical examination of pregnant woman is carried out, additionally performed are Stange's and Hench's functional respiratory tests in early period at terms of 11-19, 21-29, 31-39 weeks of gestation period, also in dynamics in I and III trimesters determined are indices of blood hormonal spectrum: T4, TSH, T3, cortisole, vitamin E, insulin, indices of lipid peroxidation and antioxidative blood activity, blood indices - hemoglobin, platelets, total protein, fibrinogen. Analysis of risk factors is carried out, their gradations and numerical values are determined and prognostic coefficients S1 and S2 are calculated by formulas. If S1>S2, presence of moderate or severe CNS lesion in children at the age of 4 is predicted. If S1<S2, presence of light degree of severity, or absence of consequences of CNS lesion in children at the age of 4 is predicted.
Method for prediction of dysthyroidism / 2446401
Method involves woman's pre-delivery blood serum examination for nitrogen oxide and relaxin that is followed by calculation of the NO/relaxin relation. If the relation is 3.6 and less, dysthyroidism is predicted.
Method of predicting development of cerebral metastases in case of lung cancer in women / 2439579
In women in tissue of lung malignant tumour after radical surgery in pulmonectomy volume and in intact lung tissue, obtained from ablated from lung section of the same lung, level of progesterone is determined. If it reduces in tumour in 5 times and more relative the level of progesterone in intact lung tissue, development of cerebral lung cancer metastases in women within the term from 3 to 8 months is predicted.
 Diagnostic technique for decompensated type 2 diabetes mellitus / 2478958
Technique involves placing a blood serum sample ~0.010 ml on a slide, dehydrating the blood serum sample for 24 hours to prepare a phase, and studying the morphology of the prepared phase through the microscope in transmitted light. Decompensated type 2 diabetes mellitus is diagnosed in a patient if the morphology shows the tongue-structure phases in number of 6 or more.
 Bacteriological diagnostic technique for helicobacter pylori in gastric mucosa of patients suffering gastroduodenal pathology / 2478957
Bacteriological diagnostic technique for Helicobacter pylori in gastric mucosa is implemented as follows. The examined material (biopsy material of gastric mucosa) is inoculated on Petri dishes containing standard medium of low sea water and agar. If small colourless colonies (of the diameter of 0.5-1.0 mm) with a smooth surface appeared to grow on the nutrient medium after 72-96 hours of incubation at temperature 36.0±1.0°C and high CO2; the colonies are microscopically examined. If the smear occurred to contain thin Gram-variable rods or coccal forms, the grown colonies are examined for catalase formation, oxidase formation, urease formation, for an ability to degrade sodium hippurate, the absence of x factor (hemin consumption) and acid resistance (the absence of growth in thioglycollate medium at pH-3.5).
 Method of evaluating element status of birds on feather / 2478956
Method involves the analysis of chemical elements content in the substrate. Feather of live bird is used as a substrate for analysis. The selection of substrate is carried out from three places: area of head, back and wings. The feather is plucked at a total weight of 1 g.
Method for prediction of cardiovascular disorders accompanying early adaptation in infants of mothers suffering arterial hypertension / 2478205
What is presented is a method for prediction of cardiovascular disorders accompanying early adaptation in infants of the mothers suffering arterial hypertension. The method is based on determining the umbilical blood concentrations of calcium ions (X1), renin (X2) and endogenous nitrite (X3) followed by calculation of a diagnostic index (DI) by formula: DI=0.2768X1+0.005X2+(-0.6990)X3-1.1317 wherein X1 is the Ca2+ concentration, mmole/l; X2 is the renin concentration, pg/ml, X3 is the endogenous NO2 concentration, mcmole/l. If DI is more than 0, the absence of laboratory signs of the cardiovascular disorder is stated, while DI less than 0 enables predicting a high risk of the developing neonatal cardiovascular disorders in the infants.
![Method of determining n-butyl ether of 2-[4-(5-trifluoromethyl pyridyl-2-oxy)phenoxy]propionic acid in biological material](http://img.russianpatents.com/img_data/1132/11326385-s.gif) Method of determining n-butyl ether of 2-[4-(5-trifluoromethyl pyridyl-2-oxy)phenoxy]propionic acid in biological material / 2477479
Analysed sample is crushed, twice infused with acetone; the acetone extract is filtered; the solvent is evaporated from the filtrate; the residue is dissolved, purified on a column with a sorbent using as the mobile phase a mixture of solvents; eluate fractions containing the analysed substance are combined and determination is carried out using a physical-chemical technique, where the residue obtained after evaporating the solvent from the filtrate is treated with acetone several times; acetone extracts are separated and combined; the solvent from the combined extracts is evaporated; the residue is purified in a column with a Silasorb S-18 sorbent, using a mobile phase of acetonitrile-water (7:3); after combining the eluate fractions containing the analysed substance, the obtained solution is diluted with water, extracted with diethyl ether; the ether extract is separated, evaporated to a dry residue; the residue is dissolved in hexane and determination is carried out via a chromatographic-mass-spectrometric technique using a capillary column of DB-1MS with length of 30 m and inner diameter of 0.25 mm with a stationary phase with thickness of 0.25 mcm, which is dimethylpolysiloxane, using helium as the carrier gas which is fed at a rate of 39 cm3/s, and a mass-spectrometric detector operating in electron impact mode, with recording of a mass spectrum on total ion current; the amount of n-butyl ether of 2-[4-(5-trifluoromethyl pyridyl-2-oxy)phenoxy]propionic acid is calculated from the chromatogram data obtained by detecting intensity of the signal due to charged particles formed when bombarding the analysed substance, coming out of the capillary column and fed into the ion source, using an ionising beam of electrons with energy of 70 eV.
Method of assessing clinical effectiveness in inflammatory intestinal diseases / 2477478
Pre- and post-therapeutic serum sE-selectin and sP-selectin are evaluated. If observing decrease of the sE-selectin level by 43.5% and more and of the sP-selectin level - by 45.9%, the therapy of inflammatory intestinal diseases is considered to be effective.
 Method for prediction of life-threatening arrhythmias in patients with type 2 diabetes mellitus suffering chronic cardiac failure / 2477477
Method involves measuring the blood glucose level (mmole/l) with the blood glucose level (mmole/l) measured at least 288 times for 24 hours. The derived data are used to calculate an average blood glucose value and a standard mean deviation from the average blood glucose value. The derived glucose values are analysed with taking into consideration glucose level variations amplitude of which exceeds the standard deviation, and an arithmetic mean value of the variations taken into consideration. If the arithmetic mean value of the variations taken into consideration is more than 5 mmole/l, life-threatening arrhythmias are predicted.
 Method for choosing management approach to pregnant women with mild arterial hypertension / 2477476
Blood is analysed for angiogenic factors: sFlt-1 - soluble fins-like tyrosine kinase and PIGF - placental growth factor. The coefficient K which is the sFlt-1 to PIGF relation is calculated. In this case, if K≥83, a management approach to pregnant women to be chosen shall use antihypertensive preparations and anticoagulants, and in case of K<83 pg/ml, the pregnant women are managed with no antihypertensive preparations used.
Method to diagnose sarcoptoidosis in animals / 2477475
Method includes taking a scrape from affected areas of animal skin, at the same time 0.1% solution of gentian violet is added to the investigated material in concentrate of dimethyl sulfoxide. Then the content is mixed and studied with a microscope to detect ticks.
Method for probable prediction of risk of developing atopia in children / 2477474
Substance of the invention consists in determining functional activity of ribosome genes localised in group D chromosomes. Herewith, a collection of personal details are to be considered and set at a value according to the patient's point rating system. All the details are substituted in formula of linear-discriminant function: y=b0+b1X1+b2X2+…+biXi, then the derived value y is substituted in equation of a probability of developing atopia P=ey/(1+ey). The derived value P in % enables predicting a probability of developing atopia in children.
Method for predicting hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis / 2244303
One should isolate DNA out of lymphocytes of peripheral venous blood, then due to the method of polymerase chain reaction of DNA synthesis one should amplify the fragments of hSERT locus of serotonin carrier gene and at detecting genotype 12/10 one should predict the risk for the development of hallucino-delirious forms of psychoses of cerebro-atherosclerotic genesis.
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FIELD: medicine. SUBSTANCE: transvaginal echography in the patients suffering endometrial polyps is conducted to measure an M-echo thickness, mm, the presence of obesity (Ob), blood follicle-stimulating hormone (FSH), IU/l, oestradiol (E2), pg/l; an insulin-resistance index is calculated by HOMA technique; a therapeutic strategy (ThStra) is taken into consideration; a probability of developing recurrent endometrial polyps (p) 1 year after the operation is determined by formula. If the probability exceeds 0.7, a high risk of recurrence is predicted. The probability falling within the range of 0.5 to 0.7 shows a moderate risk, while the probability of 0 to 0.5 shows a low risk. EFFECT: higher sensitivity of predictive diagnosis of a risk of the recurrent disease in various therapeutic strategies. 2 ex
The invention relates to medicine, namely to gynecology, and can be applied for prediction of disease recurrence in patients with endometrial polyps in postmenopausal women after surgical removal of the polyp by taking into account metabolic, hormonal disorders, gynecological status and prevention of recurrence by more destination hormonal and metabolic drugs in the postoperative period. The known method of prevention of relapse in the treatment of early endometrial cancer using agonist gonadotropin-hormone Lyukrin depot by additional destination intrauterine levonorgestrel-releasing system Mirena (RF patent No. 2428201 "Method of treatment for early endometrial cancer", publ. 10.09.2011). The disadvantage of this method is that the use of conservative therapy in endometrial cancer is very problematic due to the high cancer risk. In fact in the proposed method we are talking about prevention of uterine bleeding, and not on anti-cancer treatment. The analogue of the present invention is a method of gestalttherapie endometrial hyperplastic processes synthetic gestagens, anti-relapse activity which was increased at the expense of an individual evaluation of their binding properties of progesterone receptor of endometr the I for each individual drug (patent RF №2325911 "How gestalttherapie endometrial hyperplastic processes", publ. 10.06.2008). This purpose of endometrial biopsies were obtained cytosolic fraction, which made determination of the level of progesterone receptor radioligand method and calculate the amount of specific binding of the drug received by the progesterone receptor. While the drug of choice felt the connection with the highest value of specific binding. In the known method of anti-relapse therapy progestogen with endometrial polyps in women using endometrial biopsy and radioligand method, which does not allow to use this method as an outpatient and hinders its widespread use among medical institutions. As a prototype of the selected method, which was used for the differential diagnosis of hyperplastic processes and endometrial cancer on the basis of the calculation of the discriminant functions (DF) using the formula DF=0,00013·Comp10D1+3,98725·CStdOD2+0,00001·10D+0,01213·PERIMETR-13,4409 where Comp10D1 - integral optical density of heterochromatin nuclei of cells of the endometrium, STD OD2 - standard deviation of optical density heterochromatin, 10D - integral optical density of the chromatin of the nucleus, PERIMETR - perimeter kernel (RF patent No. 2127429 "Method for the diagnosis of hyperplastic processes and endometrial cancer", publ. 10.03.1999). When DF<O conclude on the presence of a benign process of the endometrium,and if DF> 0 diagnose endometrial cancer. The disadvantage of the study is that as the dividing point was chosen zero level when a large value in the fashion of a free member of the equation and insignificant coefficients for the indicators. The discrepancy of the order of magnitude of the coefficients and free member of the equation leads to lower diagnostic value of the method. In addition, the choice of opinion between the two diseases involves binary (there is not). As an amendment to the continuity in the case of two categorical conclusions recommend not only using multiple regression method in the calculation formula and its logit transformation, in which the formula takes the form: p=1/(1+e-y), where p is the probability of what will happen the event of interest; e is the base of natural logarithm of 2.7, y is a standard regression equation. The invention solves the problem of the optimal choice of treatment of endometrial polyps in postmenopausal women with low relapse activity. This task is achieved by the fact that postmenopausal women before treatment assess not only the local gynaecological status, and metabolic and hormonal disorders. For this purpose in patients with endometrial polyps with ultrasound transferin the flax study to determine the thickness of the M-echo in mm, the presence of obesity (Exp) (excess of body mass index 30 kg/m2) (1 point - obesity, 0 points - no obesity), blood levels of follicle-stimulating hormone (FSH) in IU/l, estradiol (E2) in PG/ml, calculate the index of insulin resistance (IR) method NOME (NOME-IR=fasting blood glucose (mmol/l)* immunoreactive insulin blood (mked/ml)/22.5 to, consider treatment strategy (Ctrl) (1 point - remove the polyp by heteroresistance, 2 points - removal of the polyp by heteroresistance appointment after surgery for 6 months agonist gonadotropin-hormone buserelina, 3 points - removal of the polyp by heteroresistance, treatment within 6 months after surgery with buserelin and Metformin), and then determine the likelihood of recurrence of endometrial polyp (p) 1 year after surgery by the formula: p=1/(1+e-(-7,38+13,95*Z)), where Z=-0,57+0,07*M-echo-0,24*Coolant-0,02*FSH+0,02*E2+0,12*IR-0,15*Ctrl, e=2,72 (the base of natural logarithm), while when exceeding probability of 0.7, the risk of re-recurrence is high, with a probability of 0.5 to 0.7 moderate and from 0 to 0.5 is low. Changing the treatment strategy chosen by the method involving the least risk of relapse. The novelty of the invention lies in the fact that the simultaneous assessment of gynecological status of the patient, metabolic and hormonal the violations, the use of multiple regression analysis, its logit transformation allows to increase the sensitivity of the predictive diagnosis of the risk of recurrence of the disease with different treatment strategies. The invention involves an inventive step, as for a specialist gynaecologist is not obvious from the level of medicine in the field of genealogie and endocrinology. In the available sources of Russia and foreign't found similar to the proposed method of predicting recurrence of polyps of the endometrium in postmenopausal women. The claimed invention is industrially applicable as there may be many times repeated and used in assessing the likelihood of recurrence of endometrial polyps in postmenopausal women and reproduced in various medical and scientific medical institutions. A method for predicting the recurrence of endometrial polyps in postmenopausal women is as follows. In postmenopausal women before treatment assess not only the local gynaecological status, and metabolic and hormonal disorders. For this purpose, patients with transvaginal sonographic examination of the internal genitalia by a standard method to determine the thickness of the M-echo in mm, measure the length of the patient in meters and weight is kg and calculate the body mass index (BMI) in kg/m 2according to the formula BMI=weight/height2. When exceeding a BMI over 30 kg/m2make a conclusion about the presence of obesity (Exp) (1 point - obesity, 0 points - no obesity). Enzyme-linked immunosorbent assay using the appropriate test systems to determine the blood levels of follicle-stimulating hormone (FSH) in IU/l and estradiol (E2) in PG/ml), glucose (Glu) on an empty stomach in mmol/l and immunoreactive insulin (IRI) on an empty stomach MCAD/ml. Then calculate the index of insulin resistance (IR) method NOME (NOME-IR=Glu·IRI/22,5). Rank proposed treatment strategy (Ctrl) (1 point - remove the polyp by heteroresistance, 2 points - removal of the polyp by heteroresistance appointment after surgery within 6 months agonist gonadotropin-hormone Buserelina depot, 3 points - removal of the polyp by heteroresistance, treatment within 6 months after surgery with buserelin and Metformin). Then determine the likelihood of recurrence of endometrial polyp (p) 1 year after surgery by the formula: p=1/(1+e-(-7,38+13,95*Z)), where Z=-0,57+0,07*M-echo+0,24*Coolant-0,02*FSH+0,02*E2+0,12*IR-0,15*Ctrl, e=2,72 (the base of natural logarithm), while when exceeding probability of 0.7, the risk of re-recurrence is high, with a probability of 0.5 to 0.7 moderate and from 0 to 0.5 is low. Changing the treatment strategy, select the way the cost is audica lowest risk of relapse. Practical application of the proposed method is illustrated by examples from clinical practice. EXAMPLES Patient S., 54 years, obesity (1 point) using ultrasound transvaginal sonography was diagnosed with endometrial polyp size 15 mm thickness of M-echo was 6 mm, the Content of FSH in the blood, the patient was 15.2 IU/l, estradiol - 37 PG/ml Index of insulin resistance, HOMA-IR was higher than normal and corresponded to 3.7. Calculate the forecast of the development of recurrence of endometrial polyp using different treatment strategies. When choosing the 1st strategy, the likelihood of relapse 0.99, and the 2nd strategy to 0.97 and 3rd strategy - 0,89. Therefore, the patient With. with the aim of preventing the recurrence of the pathological process is shown gastroretentive the polyp with the subsequent appointment buserelina and Metformin for 6 months. The patient,, 49, with endometrial polyp with transvaginal ultrasound scan determined the value of the M-echo equal to 4 mm, the body mass Index of the patients was 28 kg/m2that testified to the absence of obesity. Determination of hormones in the blood showed that the concentration of FSH in the blood was 20 IU/l, estradiol - 31 PG/ml Index of insulin resistance, HOMA-IR was normal and corresponded to 2.0. Calculate the forecast of the development of recurrence of endometrial polyp when IP is the use of different treatment strategies. When choosing the 1st strategy, the likelihood of relapse 0,0006, the 2nd strategy is 0.001 and the 3rd strategy is 0.0002. Therefore, an additional purpose buserelina and Metformin is inappropriate due to minor changes in the probability of recurrence of endometrial polyp. Patient enough, only gastroretentive polyp without therapeutic support. Technical and economic efficiency of the proposed method is that the choice of treatment of endometrial polyps in postmenopausal women with low probability of relapse will improve clinical effectiveness of operations and avoid unnecessary spending on treatment. A method for predicting the recurrence of endometrial polyps in postmenopausal women after application of different treatment strategies and accounting metabolic, hormonal and gynecological status of women, namely, that in patients with endometrial polyps at transvaginal ultrasound examination to determine the thickness of the M-echo in mm and the longitudinal size of the endometrial polyp (TL) in mm, assess the presence of obesity (RC)determine the blood levels of follicle-stimulating hormone (FSH) in IU/l and estradiol (E2) in PG/ml, calculate the index of insulin resistance (IR) method NOME (NOME-IR=blood glucose fasting (mmol/l)·immunoreactive insulin blood (MCED/m is)/22,5), consider treatment strategy (Ctrl) (1 point - remove the polyp by heteroresistance, 2 points - removal of the polyp by heteroresistance appointment after surgery within 6 months of the agonist gonado genotype releasing hormones buserelina, 3 points - removal of the polyp by heteroresistance with treatment within 6 months after surgery with buserelin and Metformin), and then determine the likelihood of recurrence of endometrial polyp (p) 1 year after surgery by the formula:
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