Method for prediction of recurrent non-small-cell lung cancer |
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IPC classes for russian patent Method for prediction of recurrent non-small-cell lung cancer (RU 2498305):
 Method for prediction of recurrent cervical cancer / 2485523
Method for prediction of recurrent cervical cancer involves biochemical daily urine analysis to determine daily urine androsterone and etiocholanolone to be related; if the relation is 0.75 mg/day or less, the recurrent disease is predicted for the first 2 years, and if the relation exceeding 0.75 mg/day, a prolonged recurrence-free period up to 10 years or more is predicted.
 Method for aromatase activity test / 2481587
Blood serum is examined for lutropin, follitropin, oestradiol, total testosterone and free testosterone levels before and 48 hours after the oral administration of 450 aromatase inhibitor, letrozole 10 mg, and the variations of lutropin and follitropin, as well as of the oestradiol/total testosterone and oestradiol/free testosterone relations are used to asses aromatase activity in points assigned by a certain way to consider low aromatase activity shown by total score 0 to 7 points, normal aromatase activity - 8 to 14 points, and high aromatase activity - 15 points or more.
Method for specifying directionality of pathological process in mucinous gastric carcinoma / 2480771
In males with mucinous gastric carcinoma after a curative resection to the extent of gastrectomy, tumor tissue is analysed for the level of testosterone. If the tissue value is 7.8±0.6 ng/g, process generalisation within 6 to 12 months is predicted; the level of tissue testosterone 174.5±15.6 ng/g enables predicting the favourable clinical course of the process, prolongation of the patient's life.
Method for prediction of recurrent endometrial polyps in postmenopausal women / 2478959
Transvaginal echography in the patients suffering endometrial polyps is conducted to measure an M-echo thickness, mm, the presence of obesity (Ob), blood follicle-stimulating hormone (FSH), IU/l, oestradiol (E2), pg/l; an insulin-resistance index is calculated by HOMA technique; a therapeutic strategy (ThStra) is taken into consideration; a probability of developing recurrent endometrial polyps (p) 1 year after the operation is determined by formula. If the probability exceeds 0.7, a high risk of recurrence is predicted. The probability falling within the range of 0.5 to 0.7 shows a moderate risk, while the probability of 0 to 0.5 shows a low risk.
Diagnostic technique for extraintestinal manifestations of celiac disease / 2476892
Blood serum is examined for prolactin and folic acid, and if the prolactin level is 30.6 ng/ml and more, while the folic acid level is 8.7 ng/ml and lower, the extraintestinal manifestations of celiac disease are diagnosed.
 Method for steroid profiling in dope test of sportsmen / 2467331
Invention refers to medicine, namely sports medicine, and may be used for steroid profiling in dope test of sportsmen. That is ensured by urine sample hydrolysis by a mixture of two enzymes: β-glucuronidase E.coli and arylsulphatase H.pomatia in volume ratio 1:1 to 1:3 in the presence of a citrated buffer solution and an internal standard. The hydrolysate is separated, derivatised that is followed by chromatic/mass-spectral analysis of the sample and recording of the derived results and stating the presence of endogenic steroids (ES). The ES value is determined in a sportsman for at least 10-15 times every 10-12 days. The analysis results are used to set minimum and maximum values of each of the derived ES. Said values are accepted to be a confidence range, and a steroid profile of the sportsman is drawn as a set of confidence ranges of all found ES.
Method of determining clinical effectiveness in cervical cancer / 2464576
One week after the termination of polychemotherapy, daily urine is examined for the amount of cortisol and cortisone, while blood is examined for adrenocorticotropic hormone. It is followed by calculating the relation of total cortisol and cortisone to adrenocorticotropic hormone. If the derived value is 0.74 and higher, high responsiveness to chemopreparations is stated.
Method for describing nature of arrested sexual development in boys in puberty with growth retardation / 2462720
Examination results are used to detect boys with the absence or insufficiency of blood concentrations of at least two of three hormones (luteinising hormone, follicle-stimulating hormone, total testosterone) to be used for the clinical course of transcranial magnetic therapy. The concentration of all hormones are evaluated in 1-1.5 months, and if observing increase of the concentrations of at least two hormones, including testosterone by min. 25% from the initial values, a functional nature of arrested sexual development is stated. If observing no increase or increase of the concentrations of two hormones, including testosterone by max. 25% from the initial values, an organic nature of arrested sexual development is stated.
 Diagnostic technique for idiopathic nasal breathing irregularities in pregnant women / 2456604
Blood serum of a pregnant woman is examined for the oestradiol concentration by enzyme-linked immunosorbent assay. Observing the oestradiol concentrations exceeding normal values according to weeks of gestation in 1.4 times and more enables stating idiopathic nasal breathing irregularities accompanying a period of pregnancy.
Method for prediction of level of efficiency of reduction of manifestation severity of atopic dermatitis / 2453851
Pre-therapy blood plasma is analysed for immunoglobulin E, adrenocorticotrophin and endorphin, the index SCORAD dynamics is determined by formula: Dsk=1.34147-0.000820741*IgE-0.00382642*ACTP+0.0209401*endorphin wherein IgE is the level of acute blood plasma immunoglobulin E mIU/ml; ACTP is the level of acute blood plasma adrenocorticotrophin ng/l. If observing the Dsk value exceeding 1.18, a prognosis of improving clinical dynamics of recovery is favourable; the conventional therapy is considered to be sufficient. The Dsk value being equal to 1.18 or less, the improving clinical dynamics of recovery is unfavourable, the intensified therapeutic scheme for atopic dermatitis is considered to be preferential.
Method for determining titanium, aluminium in cadiovascular tissues / 2498304
Invention described a method for determining Ti, Al in the cardiovascular tissues, involving placing a tissue sample into a container with added nitric acid, and keeping for 3 hours at 75°C; that is followed by adding hydrogen peroxide, keeping for 2 hours at the same temperature, adding a new portion of nitric acid, keeping for 2 hours at temperature 110°C, adding fluohydric acid and keeping for 18 hours at 20°C, heating for 6 hours at 75°C and placing into a microwave, boiling off and measuring by inductively coupled plasma mass spectrometry at the following ration of the ingredients, wt %: HNO3 35 wt %, H2O2 5 wt %, HF 13.3 wt %, deionised water - the rest.
Method for detection of infectious complications in patients with acute lymphoblastic leukaemia accompanying neutropenia / 2497129
Invention refers to a laboratory diagnostic technique for developing an infection in the patients with acute lymphoblastic leukaemia (ALL) in the state of induced neutropenia. Substance of the method consists in patient's blood analysis for C-reactive protein (CRP) count, and if observing no clinical manifestations of the infection with the CRP count <11 mg/l, no infection is detected, while the CRP count >11 mg/l enables diagnosing the infectious process independent from fever or a site of infection.
 Method for prediction of population biotransformation disorders of foreign substances caused by exposure to man-induced chemical habitat factors / 2497120
What is presented is a method for prediction of the population biotransformation disorders of foreign substances caused by the exposure to man-induced chemical habitat factors. One ethnic population living on the territory of the exposure to hazardous chemical factors is sampled; buccal epithelium is sampled; DNA is recovered that is followed by genotyping of the polymorphous version 9893A/G of CYP1A1 gene, 921A/C of CPOX gene, and G308A of TNF-alpha gene; one of the following gene conditions are stated: heterozygous, normal homozygous or minor homozygous; a prevalence ratio of the minor alleles for the genes studied is calculated by formula; and the population disorders are predicted.
Method of choosing therapeutic approach to patients with acute destructive pancreatitis / 2497119
Blood serum is examined for the concentration of procalcitonin (PCT) using immunoenzymometric assay. In addition, a severity of patient's condition is assessed by SAPS scale. If the PCT level is no more than 2 mcg/l, while the SAPS assessment is less than 5 points, what is chosen is an conservative therapeutic approach using the full-scale integrated intensive treatment; if the SAPS assessment exceeds 5 points, an integrated intensive treatment is combined with a diagnostic and treatment laparoscopy. If the PCT level exceeds 2 mcg/l, while the SAPS value is assessed to be less than 5 points, a limited-incision surgery is preferred to manage a local infection process, while an extensive process requires an open laparotomy; if the SAPS assessment exceeds 5 point, a surgical intervention is recommended, specifically a puncture drainage operation followed by the limited-incision surgery for the local process, while the extensive process is managed by the puncture drainage operation followed by the open laparotomy.
 Fast method for treating intestinal smooth muscle cells with bacillus cereus hemolysin ii / 2497118
Cells are treated in the same sample with pore-forming Bacillus cereus Hlyll hemolysin in the concentration of 3-5 mcg/ml.
 Differential diagnostic technique for bronchial asthma / 2497117
What is presented is a differential diagnostic technique for severe bronchial asthma, including defining the pulmonary ventilation function and analysing mononuclear cells recovered from patient's venous blood that is followed by calculation of an index characterising the inflammatory process. The monocytes are cultured into dendritic cells. A half of the cultures are added with N-athylcarboxamidoadenosine (NECA). The prepared dendritic cells are collected and analysed for the mRNA IL-8 content by polymerase chain reaction with reverse transcription. A probability P of referring the individual to a group of the patients suffering bronchial asthma is calculated by system of equations. If P>0.5, severe bronchial asthma is diagnosed.
Method for prediction of developing perivascular disorders in patients suffering influenza / 2496111
Patients suffering influenza are examined for an age group, a follow-up period and blood serum thrombocyte aggregation with ristomycin in seconds. The derived data are used in formula: PC=11.4+A*0.28-B*0.51-C*0.73, wherein: PC is a predictive coefficient of a risk of developing perivascular disorders; 11.4 is a constant for mathematical calculation of predicting the developing perivascular disorders; A is the patient's age group, wherein: group 1, if the patient's age falls within the range of 21 to 35 years old; group 2, if the patient's age is between 36 and 50 years old; and group 3 is for the patients between 51 to 65 years old; B is the follow-up period. 1 - period 1 - 1st-3rd days of the disease, 2 - period 2 - 4th-5th days of the disease, 3 - period 3 - 6th-8th days of the disease, 4 - period 4 - 9th-14th days from the beginning of the disease, 5 - period 5 - one month from the beginning of the disease; C is blood serum thrombocyte aggregation with ristomycin in seconds.
Method for prediction of smouldering form of chronic lympholeukaemia / 2496110
That is followed by genetic typing of the polymorphous locus -248G>A of Bc1-2 gene of the associated X protein (BAX -248G>A). If observing the genotype GG in the patient, a higher probability of the developing smouldering form of chronic lympholeukaemia is predicted.
 Method for preparing sample for gas chromatographic urine thioglycolic acid test / 2496109
What is described is a method for preparing sample for gas chromatographic urine thioglycolic acid test involving derivatisation, ethyl acetate recovery of thioglyolic acid from the sample, wherein thioglycolic acid is derivatised by methanol in the presence of concentrated sulphuric acid at temperature 80°C for 15 minutes, while the same is recovered from the sample by ethyl acetate by liquid-liquid micro extraction for 5 minutes with all the operations performed in the same container.
Method for evaluating patient's infection by tick-borne encephalitis virus / 2495428
What is presented is a method for evaluating the patient's infection by tick-borne encephalitis virus, on the basis of detecting virus RNA by real-time PCR.
Method for evaluating the severity of pathological process flow at chronic diffuse hepatic lesions / 2244305
One should detect the level of hepato-specific enzymes (HSE) in blood plasma, such as: urokinase (UK), histidase (HIS), fructose-1-phosphataldolase (F-1-P), serine dehydratase (L-SD), threonine dehydratase (L-TD) and products of lipid peroxidation (LP), such as: dienic conjugates (DC), malonic dialdehyde (MDA). Moreover, one should detect the state of inspecific immunity parameters, such as: immunoregulatory index (IRI) as the ratio of T-helpers and T-suppressors, circulating immune complexes (CIC). Additionally, one should evaluate the state of regional circulation by applying rheohepatography (RHG), the system of microhemocirculation with the help of conjunctival biomicroscopy (CB) to detect intravascular index (II). In case of increased UK, HIS levels up to 0.5 mcM/ml/h, F-1-P, L-SD, L-Td, LP products, CIC by 1.5 times, higher IRI up to 2 at the norm being 1.0-1.5, altered values of regional circulation, increased II up to 2 points at the norm being 1 point, not more one should diagnose light degree of process flow. At increased level of UK, HIS up to 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 1.5-2 times, increased IRI up to 2.5, altered values of regional circulation, increased II up to 3-4 points one should diagnose average degree of process flow. At increased level of UK, HIS being above 0.75 mcM/ml/h, F-1-P, L-SD, L-TD, LP products, CIC by 2 and more times, increased IRI being above 2.5, altered values of regional circulation, increased II up to 5 points and more one should diagnose severe degree of process flow.
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FIELD: medicine. SUBSTANCE: what is involves is the histological examination of tissue fragments taken from the extracted lung with primary, intermediate and segmental bronchi at 4-5 cm from the tumour, and disregeneration change cases are determined in the respiratory bronchial epithelium, including: basal cell hyperplasia (BCH), squamous metaplasia (SM) and if observing a combination of basal cell hyperplasia and squamous metaplasia (BCH+SM+) in the respiratory bronchial epithelium adjacent to the tumour, a risk of developing the recurrent non-small-cell lung cancer is predicted. EFFECT: using the declared method enables higher accuracy and information value of the prediction of the recurrent non-small-cell lung cancer. 1 tbl, 4 ex
The invention relates to medicine, specifically to Oncology, and relates to methods for predicting the occurrence of relapse in non-small cell lung cancer. Lung cancer remains one of the major cause of death among cancer patients worldwide. Even after radical treatment in 60-75% of patients with small cell lung cancer (NSCLC) is the progression of cancer, which can be in the form of local recurrence, lymphogenous metastasis in the thoracic cavity or the development of distant metastases [1, 6]. From timely detection of recurrence depend on the treatment efficiency and the life expectancy of patients. Therefore, an urgent task is not only the search for methods of early detection of recurrence, but also the prediction of their development. There are various factors influencing the occurrence of relapse in NSCLC. The dependence of the frequency of recurrence of the tumor, the stage of the disease [7, 17]. One of the adverse factors associated with high risk of locoregionally of relapse in NSCLC is the presence in patients with diabetes [17]. Great influence on the frequency of recurrence have vascular invasion and spread of tumor cells in the visceral pleura [10]. Great attention is paid to study the prognostic value of molecular-g the kinetic markers, defined in the tumor. For this purpose, methods are used CIS, PCR and immunohistochemical tissues diagnostics. Using PCR method shows the relationship between the violation of miRNA-mediated regulation of genes and progression of NSCLC[3, 4, 5, 8, 11, 14, 15, 16, 18]. It should be noted that the prognostic markers that can predict the recurrence of NSCLC, still not defined [13]. With a higher frequency of recurrence in NSCLC is associated with a high degree of malignancy [2]. Closest to the proposed method is described by T. Nakagawa (2008), according to which to adverse factors contributing to the occurrence of relapse in NSCLC include less surgical intervention than lobectomy, no Lipovskii, tumour size more T2 [12]. Nevertheless, Jung-Jyh Hung and Yu-Chung Wu (2012) analyzed the publications related to the prognostic factors of recurrence in NSCLC noted that they are inadequate for effective forecasting [7]. Thus, currently known clinical and morphological criteria for the prediction of recurrent NSCLC belong exclusively to the parameters of the primary tumor. Virtually no methods for predicting the probability of relapse in NSCLC, based on the definition of the characteristics of disregenerative respiratory epithelium adjacent to tumor lung tissue A new technical challenge-improving the accuracy and informative way. To solve the problem in the method for predicting the likelihood of relapse in non-small cell lung cancer, which consists in taking into account the clinical characteristics, conduct histological examination of tissue fragments remote light with the main, intermediate or segmental bronchi, located at a distance of 4-5 cm from the tumor, in the respiratory epithelium of the bronchi determine options dysregulatory changes: basal cell hyperplasia (BCG), squamous metaplasia (PM) and in the presence adjacent to the tumor epithelium of the bronchi combination of basal cell hyperplasia with squamous metaplasia (BCG+PM+) predict the risk of recurrence of non-small cell lung cancer The method is as follows. ...Take into account the clinical features, which include: less surgical intervention than lobectomy, no Lipovskii, tumour size more than T2. Material for morphological tumor is located from it at a distance of 4-5 cm pieces of fabric remote light with the main, intermediate or segmental bronchi. Tissue fixed in neutral formalin, carried out by a standard method and salivarium in paraffin. Sections of the bronchi thickness of 5-6 mcmorial with hematoxylin and eosin. Morphological study carried out with the help of light microscope Axiostar plus" company "K. Zeiss - Germany and performed histological examination of tissue fragments remote light with the main, intermediate or segmental bronchi, located at a distance of 4-5 cm from the tumor, in the respiratory epithelium of the bronchi, identify options dysregulatory changes: hyperplasia of goblet cells (GBq), basal cell hyperplasia (BCG), squamous metaplasia (PM) and in the presence adjacent to the tumor epithelium of the bronchi combination of basal cell hyperplasia with squamous metaplasia (BCG+PM+) predict the risk of recurrence of NSCLC. The method is illustrated by the following examples. Example 1. Patient M., 58 years. Central cancer of the right lung Operated in the amount of pneumonectomy right, without preoperative chemotherapy. When Example 1. Patient M., 58 years. Central cancer of the right lung Operated in the amount of pneumonectomy right, without preoperative chemotherapy. Histological examination of the tissue of the primary tumor diagnosed squamous cell carcinoma moderately differentiated non-squamous cell lung cancer, stage T2N1M0. Study according to the proposed method. According to the results of microscopic examination of histological material in respiratory is petelia bronchi, the border of the tumor identified areas of epithelium with evidence of BCG and PM (BCG+PM+). Dynamic observation of patients after 32 months after surgery had developed a relapse. Example 2. Patient W., 52 years old. Central cancer of the left lung. Operated in the amount of pneumonectomy left. In the preoperative period held neoadjuvant chemotherapy according to the scheme: paclitaxel 175 mg/m2in/in 1 day + carboplatin AUC 6 in/in 1 day, 2 courses with an interval of 3 weeks. Histological examination of the tissue of the primary tumor diagnosed squamous cell carcinoma moderately differentiated non-squamous cell lung cancer, stage T3N0M0. Study according to the proposed method. Microscopic examination of histological material in the respiratory epithelium of the bronchi adjacent to the tumor were detected combination dysregulatory processes BCG and PM (BCG+PM+). Dynamic observation of patients after 27 months after surgery had developed a relapse. Example 3. Patient T., 62. Peripheral cancer of the lower lobe of the right lung. Operated in the volume of the lower lobectomy right, without preoperative chemotherapy. Histological examination of the tissue of the primary tumor diagnosed adenocarcinoma moderate degree of differentiation, stage T1N0M0. Not metastatic nature adenocarcinoma l is Gogo confirmed tissues study. Study according to the proposed method. Microscopic examination of sections of the respiratory epithelium of the bronchus, the border of the tumor was identified combination dysregulatory processes: BCG and PM (BCG+PM+). Dynamic observation of patients after 25 months after surgery had developed a relapse. Example 4. Patient P., aged 54. Central cancer proximal bronchus on the right. Operated in the amount of pneumonectomy right, without preoperative chemotherapy. Histological examination of the tissue of the primary tumor diagnosed squamous non-squamous cancer moderate degree of differentiation, stage T3N1M0. Study according to the proposed method. Microscopic examination of sections of the respiratory epithelium of the bronchus, the border of the tumor was revealed hyperplasia of goblet cells (BCG-PM-). Dynamic observation of patients within 36 months of the signs of the progression of the disease is not checked. Criteria were chosen as a result of data analysis of clinical observations. Studied surgical material from 112 patients with non-small cell lung cancer stage T1-4N0-xM0at the age from 41 to 73 years. In the studied sample was dominated by men - 96 (88.1 per cent), women respectively was 13 (11.9 percent). In preparati nom period of 48 patients (42.8 per cent) received NACHT scheme: paclitaxel 175 mg/m 2in/in 1 day + carboplatin AUC 6 in/in 1 day, 2-3 courses with an interval of 3 weeks. During radical surgery 30 patients (27.5 per cent) once received IORT dose of 10-15 Gy, 18 patients (60%) before irradiation was introduced radiosensibility cisplatin/Gemzar. Morphological diagnosis of lung cancer was defined according to "Histological classification of tumors of the lung" (who, Geneva, 2003). Preneoplastic (D I) power and neoplastic processes (D II-III degree) was evaluated according to the criteria outlined S. Lantue' joul et. al. [9]. 72 people (64,3%) were diagnosed with squamous cell carcinoma, 40 (35,7%) lung adenocarcinoma different degrees of differentiation. Squamous cell carcinoma was more common in males 69 people (61,6%). All women included in the study were found to have adenocarcinoma of various degrees of differentiation. Material for morphological studies were swelling and held her at a distance of 4-5 cm pieces of fabric remote light with the main, intermediate or segmental bronchi. Tissue was fixed in neutral formalin, was conducted by a standard method and filled in paraffin. Sections of the bronchi thickness of 5-6 μm were stained with hematoxylin and eosin. Morphological study was carried out with the help of light microscope Axiostar plus" company "K. Zeiss - Germany. For whom adoption is not metastatic nature of adenocarcinoma of the lung was performed immunohistochemical study. Used a panel of antibodies, including TTf-1 (Dako clone), SK (clone OV-TL 12/30, Novocastra), CK20 (clone Ks20.08, Novocastra), CEA (clone AMT28, Novocastra), CDX2 (clone AMT28, Novocastra). Immunohistochemistry was performed according to the standard technique. Demeterova antigens was carried out by incubation of the sections in a microwave oven for 20 min in citrate buffer or EDTA-8. As Chromogen was used, diaminobenzidin (DUB). Visualization of the reaction antigen - antibody was performed using the system "Super Sensitive Polymer - HRP Detection System)) (Bio Genex, USA). Statistical processing of results was performed using the software package Statistica 6.0 for Windows. To assess differences between groups in the frequency of occurrence of the symptom criteria were used χ2. Defined range dysregulatory changes occurring in the areas of respiratory epithelium adjacent to the tumor. According to the results of microscopic examination of histological material in the respiratory epithelium of the bronchi, the border of the tumor was observed various options dysregulatory changes - GBq, BCG, PM and dysplasia of varying severity. Data dysregulatory changes met as independent processes, and in various combinations within the investigated fragment of tissue. All patients were divided into groups of basal cell hyperpl the Oia in respiratory epithelium in combination with squamous metaplasia and without it. Of the 112 patients with NSCLC, 81 (72.3 per cent) adjacent to the tumor bronchial epithelium was observed BCG. While in 29 patients (35.8 per cent) of BCG combined with squamous metaplasia (BCG+PM+). Assessed the relationship of nature disregeneration changes observed in adjacent to the tumor sites of the mucosa of the bronchus, with the incidence of recurrence. It has been found that squamous cell lung cancer relapses in 26.1% of cases occurred when adjacent to the tumor sites bronchial epithelium met type of disregenerative BCG+PM+ and only 3.3% for isolated BCG ((BCG+y); χ2=4,39; p=0.03). In cases of lung adenocarcinoma, if in the border of the tumor areas of the bronchial epithelium was observed in the combination of BCG+PM+, relapses occurred in 50% of cases. When analyzing the frequency of occurrence of relapse in NSCLC depending on the nature of disregenerative, noted that it is in indirect communication with the combination of BCG+PM+(χ2=23,5; p=0,0000; table 1). Dynamic observation of patients during 36-42 months showed that in those cases, when in the border of the tumor epithelium of the bronchi were determined other types dysregulatory changes, the risk of recurrence is much lower. Sensitivity 90%; specificity 80%. Thus, the proposed method can more accurately predict Vozniknovenie the relapse in NSCLC, that will allow you to optimize the management of patients. Sources of information
A method for predicting the likelihood of relapse in non-small cell lung cancer, which consists in taking into account the clinical characteristics, characterized in that conduct gistologicheskikh fragments of tissue remote light with the chief, intermediate or segmental bronchi, located at a distance of 4-5 cm from the tumor, in the respiratory epithelium of the bronchi determine options dysregulatory changes: basal cell hyperplasia (BCG), squamous metaplasia (PM) and in the presence adjacent to the tumor epithelium of the bronchi combination of basal cell hyperplasia with squamous metaplasia (BCG+PM+) predict the risk of recurrence of non-small cell lung cancer.
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