Method for preparing saponin-containing extracts (versions). RU patent 2510278.
|
Method for preparing cake shelf fungus extract / 2509567 Invention refers to pharmaceutical, food and cosmetic industry and concerns preparing a cake shelf fungus extract. The method for preparing the cake shelf fungus extract involving freezing cake shelf fungus, wetting it with a water solution containing dimethyl sulphoxide and sodium hydroxide, with a solution temperature being 50-90°C, and keeping at the above temperature for 1-2 hours. |
 Agent having hypoglycemic and anti-inflammatory activity / 2506091Invention refers to pharmaceutical industry, namely an agent having hypoglycemic and anti-inflammatory activity. The agent having hypoglycemic and anti-inflammatory activity is prepared by extraction of a herbal material consisting of a mixture of ground common burdock roots and rhizomes; common dandelion roots; horseheal roots and rhizomes; bean shoots; bilberry shoots; cinquefoil shoots and common nettle leaves in 40% ethanol in the certain environment; the combined extracts are filtered, evaporated, purified by separation, dried and ground. |
 Dry fucus extract, method for preparing it, and base anticoagulant ointment / 2506089Invention refers to pharmaceutical industry, namely an agent possessing anticoagulant activity. A method for preparing a dry fucus extract possessing anticoagulant action by the complex treatment of Fucus vesiculosus. The dry fucus extract possessing anticoagulant activity representing a polysaccharide complex containing fucoidan having the following composition: neutral monosaccharides - fucose, xylose, mannose, galacose, glucose, uronic acid; as well as polyphenols and sulphates in specific amount. The anticoagulant ointment containing the dry fucus extract. |
|
Method of obtaining rutin / 2505307 Method of obtaining rutin from vegetative mass of buckwheat includes the following stages: dried, crushed buckwheat is subjected to extraction with 70% ethyl alcohol, thickened, filtered, dried, purified from admixtures by means of organic solvents, dissolved, subjected to hot filtration, crystallised, with crushed vegetative buckwheat mass being before extraction subjected to uncoiling, extraction with 70% ethyl alcohol on boiling water bath, filtration and re-extraction with 70% ethyl alcohol, re-filtration, obtained alcohol extracts are concentrated on water bath under vacuum and dried, twice purified from admixtures by means of organic solvents, as organic solvents used are diethyl ester and ethylacetate. After crystallisation, re-crystallisation is carried out with hot 70% ethyl alcohol with hot filtering through paper filter, with further cooling until rutin crystals precipitate, filtration under vacuum and drying in the open air. |
 Method for preparing herbal agent possessing anticoagulant, hypotensive and diuretic action / 2504391Invention refers to pharmaceutical industry, namely a method for preparing an agent possessing anticoagulant, hypotensive and diuretic action. The method for preparing the agent possessing anticoagulant, hypotensive and diuretic action, consisting in the fact that milled dark mullein is extracted in 60% ethanol by re-percolation in a cluster of 3 unequally batched percolators; the prepared extract is settled, filtered, boiled down and dried in a vacuum drier in the certain environment. |
|
Method for producing chromogenic shelf fungus complex / 2502516 Invention refers to method for producing a chromogenic shelf fungus complex, wherein milled shelf fungus is wetted in ratio (0.5-1.5) - (3.5-4.5), frozen at temperature minus 16±3°C for 1-4 hours, settled for 1-2 hours at temperature 65-75°C, filtered; the filtrate is acidified in 25% hydrochloric acid to pH 1.0-2.0, mixed, settled for 12-15 hours and filtered. |
 Method for preparing chlorophyll concentrate of sugar laminaria possessing antimicrobial and wound healing action / 2500413Invention refers to medicine, namely to pharmaceutical industry, concerns preparing a chlorophyll concentrate of sugar laminaria. A method for preparing the chlorophyll concentrate of grinded sugar laminaria by three extractions in ethanol in a shadowed place; further, cake extract is separated; the extractants are combined; the extragent is distilled; the residue is added with water, filtered through a paper filter; the filtered residue is washed in two-three portions of water, dissolved in 96% ethanol; total chlorophylls are dissolved in sterile olive oil to produce 25% total chlorophyll concentration, residual 96% ethanol is distilled under vacuum; the method is conducted in the specific environment. |
 Method for preparing agent possessing immunomodulatory activity / 2496510Invention refers to pharmaceutical industry, namely a method for preparing an agent possessing immunomodulatory activity. The method for preparing an agent possessing immunomodulatory activity characterized by the fact that herbal composition containing common motherwort herb, knotgrass herb, pot marigold blossom, licorice rhizome and root, Alexandrian laurel rhizome and root, rosehip, schizandra fruit, linseeds, sequentially twice extracted in 60-70% ethanol, twice in 40-50% ethanol, and once in purified water under specific conditions; then the aqueous-alcoholic extracts are concentrated in vacuum; the aqueous still residues are combined with the aqueous extract, then filtered, boiled out, purified by separation, boiled out additionally, dried in a vacuum dryer and further milled. |
 Method for preparing agent possessing tonic activity / 2496509Invention refers to pharmaceutical industry, namely a method for preparing an agent possessing tonic activity. The method for preparing the agent of true ginseng roots, possessing tonic activity by three extractions of the ground raw material in 40-80% ethanol with using three extraction vessels in the specific environment; at first the extraction process takes place at room temperature for 12 hours, while the third extraction is conducted at temperature 90°C for 30 minutes in each extraction vessel. |
 Method of obtaining vegetable preparation, which has diuretic and anticoagulation activity / 2491951Invention relates to pharmaceutical industry and deals with creation of medication of vegetable origin based on biologically active substances of Linaria vulgaris, which has diuretic and anticoagulation activity. Method of obtaining medication, which has diuretic and anticoagulation activity, consists in the following: extraction of milled grass of Linaria vulgaris with ethyl alcohol is carried out with successive drawing with 3 parts of extractant by method of remaceration with heating and connecting vacuum at the stage of discharge, under specified conditions. |
 Immunostimulating drug / 2510277Invention refers to an immunostimulating drug on the basis of a herbal alcohol tincture. The above drug is prepared by filling the herbal raw material with 70% ethanol in the ratio of 1:10, keeping in a dark place at temperature 18-20°C for 7 days, filtering, extracting the raw material, and filtering the extract. The herbal raw material contains purple Echinacea herb and blossom clusters, Scotch pine buds, horseheal roots and rhizomes, common licorice roots and harmel herb taken in 1:1:1:1:0.5. |
|
Method for preparing pectin gel of elevated part of willow herb / 2510275 Invention refers to a method for preparing a pectin gel of an elevated part of willow herb. The declared method enables dissolving pre-recovered pectin from the elevated part of willow herb in water, cooling and applying onto a frozen chitosan solution and further gel formation. |
|
Oral composition / 2510262 Group of inventions refers to medicine, namely to dentistry, and may be used as an oral composition for controlling an oral sweetener release. The above composition contains a polymer attached to the sweetener by a breakable link comprising a covalent bond, an ester bond or an amide bond. The breakable link is breakable in the oral cavity to release the sweetener. Said polymer is a polymer adhered to the mucosa and specified in methyl vinyl ether/maleic anhydride copolymer with polymer found in the amount of 0.001% to 10%. The sweetener is specified in dextrose, saccharose, maltose, dextrin, dried invert suger, mannose, xylose, ribose, fructose, lexulose, galactose, corn syrup, partially hydrolysed starch, sorbitol, mannitol, isomalt, aspartame, neotame, saccharine and its salts, sucralose, the sweeteners on the basis of dipeptides, cyclamates, dihydrochalcone with the sweetener content making 0.005% to 5%. What is also presented is a portable dispenser, a method for preparing the oral composition, a method for controlling the sweetener in the oral cavity, and using the polymer. |
|
Composition for treating and preventing osteoarthritis and osteoarthrosis / 2509569 Invention refers to pharmaceutical industry, namely to a composition for treating and preventing osteoarthritis and osteoarthrosis. The composition for treating and preventing osteoarthritis and osteoarthrosis contains a powder or an extract of a dry plant specified in a group: burdock, dandelion, cowberry, birch, St.-John's wort, golden-rod, nettle, peppermint, licorice, cinquefoil, tormentil, brier, Greek valerian, valerian, corn, holy thistle, oat, agrimony, everlasting flower, ginseng, sage, starwort, squash, willow, wild strawberry, chicory, wheat-grass, artichoke, bilberry, marigold, horse radish, garlic, aspen, knotgrass, plantain, inula, Fagopyrum rubricaulis, chamomile, balm lemon, blue poppy, cudweed, tripartite bur-marigold; a male larval bee lyophilisate and a quercetine or dihydroquercetine, or rutin powder in certain proportions. |
|
Method for correction of total blood serum baee-esterase activity using soya protein isolate / 2509568 Method refers to preventive medicine and concerns decreasing total BAEE-esterase activity. For this purpose, soya protein isolate 30 gram has been daily introduced into the subjects for 2 months. |
|
Method for preparing cake shelf fungus extract / 2509567 Invention refers to pharmaceutical, food and cosmetic industry and concerns preparing a cake shelf fungus extract. The method for preparing the cake shelf fungus extract involving freezing cake shelf fungus, wetting it with a water solution containing dimethyl sulphoxide and sodium hydroxide, with a solution temperature being 50-90°C, and keeping at the above temperature for 1-2 hours. |
 Compositions of borinic acid / 2509553Invention refers to pharmaceutical industry and represents an oral care composition having pH min. 8 or buffered so that to maintain the pH value min. 7, containing 3- hydroxypyridine-2-carbonyloxy-bis-(3-chlor-4-methylphenyl)borane in a combination or together with an orally acceptable carrier. |
 Composition applicable as personal hygiene product and methods for using it / 2509552Invention refers to cosmetology and represents a composition applicable by a user as a personal hygiene product in the form of a bath or shower gel, containing: (a) no more than 11.5 wt % of an anionic surfactant representing alkyl sulphate, alkyl sarcosinate, or any combination thereof; (b) no more than 3 wt % of a zwitterionic surfactant representing fatty acid amidoalkylbetaine, (c) not less than 0.1 wt % of a hydroxyalkylcellulose derivative with a long-chain alkyl group; and (d) water. |
|
Method of human health improvement / 2509547 Invention refers to medicine, namely recreation therapy, and may be used for human health improvement. That is ensured by traditional medical examination of the patient. The findings are used to draw up the rehabilitation program including body cleansing, intestine and liver purification, physical exercises. One week before the beginning of the rehabilitation program, the patient starts separate nutrition with preferential vegetable food and dairy products, limited consumption of salt, alcohol and sugar. One day before the rehabilitation program, a fasting day is kept with taking 1% kephir or fresh apple juice. Further, on the first two days of the three-day program or on the first four days of the seven-day program, the body is cleansed by keeping a juice diet. The juice diet contains fresh vegetable and fruit juices, herbal infusions and mineral water. Water is unlimited. On the first day of the cleansing program, the intestine is purified with the preparation Fortrans. Starting from the first day the liver and gall bladder are cleansed with using herbal and saline choleretics for four days of the 7-day program and for two days of the 3-day program. That is combined with paradoxical respiration by Strelnikova's technique and hydrotherapy. Physical exercises involve physical loads on a cardio-vascular machine, one training as prescribed by the doctor - yoga, pilates, chi gong, water aerobics or hypoxi capsule. Therapeutic massage and lymph drainage massage follow. Then body-detox, ozone therapy, collagenarium procedures are performed. Cold training procedures involve Linear Kneipp, saunas, swimming pool, turpentine bathes. Bathing and cold training procedures are followed by pilling and algae wraps. From the 5th day of the 7-day program, or from the 3rd day of the 3-day program, the diet involves water cereals, vegetable and fruit salads, vegetable fast soups. |
|
Adhesive ointment for treating oral mucosal involvements in lichen acuminatus / 2508120 Invention refers to pharmaceutical industry, namely to an agent for treating an oral mucosal involvement accompanying lichen acuminatus. An adhesive ointment for treating the oral mucosal involvements accompanying lichen acuminatus containing sea buckthorn oil and 0.5% prednisolone ointment, socoseryl dental adhesive paste and vitamin A in the specific proportions. |
 Amino acid preparation eliciting antitumor effect and method for its preparing / 2245143Invention relates, in particular, to the development of an antitumor preparation based on natural substances. Invention relates to an amino acid preparation comprising at least one modified essential amino acid obtained by treatment of amino acid by ultraviolet radiation (UV) at wavelength 250-350 nm for 12-80 h at temperature 15-30oC or with ozone at temperature 15-25oC. The modified amino acid has no toxicity for health cells. Also, invention relates to a method for preparing such preparation. Invention provides the development of an antitumor preparation based on modified amino acids and expanded assortment of antitumor preparations being without cytotoxicity for normal cells. |
FIELD: medicine, pharmaceutics.
SUBSTANCE: invention refers to pharmaceutical industry, namely to a method for preparing a saponin-containing extract. The method for preparing the saponin-containing extract containing pre-soaking of Saponaria officialis L. roots in distilled water, ultrasonic extraction, filtration in the specific environment.
EFFECT: method provides higher quality of the end product and reducing a time for preparing the saponin-containing extract from Saponaria officinalis L.
5 ex
The invention relates to the chemical-pharmaceutical and medical industry, namely to a method of obtaining biologically active substances from plant raw materials.
A method of obtaining saponins ivy (RF Patent №2395516, C07H 1/08, C07H 15/256, A61K 36/25, priority 18.12.2008) by extraction of the containing vegetable raw materials in airtight conditions in the aquatic environment or in an environment of water 0,5-5% ammonia solution at a temperature of 110-200 C.
The main disadvantages of this method are conducting extraction at high pressure and high temperature, which significantly increases the cost of the extraction process, and the use of ammonia as a solvent.
Also a method of obtaining saponins soybean (Patent KR №20030059028, C12P 19/44, priority 13.06.2003), which implies that water extraction and use of treated by aqueous extract of the enzyme beta-glucosidase.
The disadvantages of this method is the low yield of the desired product and the use of expensive enzyme for the treatment of the extract.
Closest to the proposed method is a method of ultrasonic extraction of saponins from the roots of ginseng (J. Wu, L. Lin, F. Chau Ultrasound-assisted extraction of ginseng in saponins from ginseng roots and cultured ginseng cells. Ultrason. Sonochem. 2001. Vol.8. P.347-352), based on the destruction of the internal cellular structure and release the contents of the cells to accelerate mass transfer. The effect is achieved by fast selective heating of raw material in the solvent, partially transparent to the mechanical action of acoustic cavitation.
The disadvantage of this method is the use of organic solvents and duration of extraction (>2 hours).
The objective of the invention is intensification of the process of obtaining capodistrias extracts and reduction of the costs of extraction.
The technical result of the invention is to increase the quality of the desired product using non-toxic solvent (water)and reducing the time of receipt capodistrias extracts from Saponaria officinalis L.
The task and this technical result is achieved by the fact that a method for obtaining capodistrias extracts, including soaking roots Saponaria officinalis L. in distilled water, ultrasonic extraction, filtration extract from vegetable raw materials, according to the invention, the extraction was performed under the influence of ultrasound with the frequency of 30 kHz and the intensity of 228% to 232 W/cm 2 , for 19% to 21 minutes with preliminary soaking raw materials in distilled water for 19% to 21 minutes. The ultrasound is performed at a temperature of 25 C with a ratio of vegetable raw materials and water 1:5.
The use of ultrasound with the frequency of 30 kHz allows to considerably reduce the time needed to obtain capodistrias extracts. When you use ultrasound with frequency below 30 kHz indicators capodistrias extracts worse, the use of ultrasound with the frequency of above 30 kHz impractical due to excessive energy consumption.
The use of ultrasound intensity 228% to 232 W/cm 2 provides the reduction of time of extraction. When using intensity of ultrasonic affecting is less than 228 W/cm 2 increases the time to achieve optimal yield and increase in intensity over 232 W/cm 2 leads to the deterioration get capodistrias extracts.
Time of ultrasonic influence 19 to 21 minutes is optimal for obtaining capodistrias extracts with the best results (in dry substance and foam-making abilities). Reducing the time of exposure of less than 19 minutes is not possible to achieve optimum output of the extract and the increase of time of ultrasonic processing more than 21 minutes leads to the destruction allocated saponins.
Process temperature (25 C) selected experimentally, at a given temperature optimizes performance capodistrias extracts.
Pre-soaking of plant raw materials (19% to 21 minutes) selected experimentally, as this time soaking optimizes performance capodistrias extracts.
The proposed ratio of vegetable raw materials and water is the most appropriate, as this ratio provides the most complete removal of saponins from vegetable raw materials.
The method of obtaining capodistrias extracts was performed as follows. Hanging plant materials were placed in chemical glass and filled with distilled water (water duty 1:5), after which the raw materials were left to soak for 19% to 21 minutes. Ultrasonic generator tuned according to the intensity, then cap the ultrasonic generator immersed in a glass of vegetable raw materials and conducted the processing of raw materials. After processing is complete, the solution was filtered through a porous glass filter and determined the amount of the obtained extract. For the obtained extracts were determined by the content of dry substances of refraction and gravimetric methods and foaming ability.
Optimal conditions for extraction was determined experimentally by the content of dry substances in the extract and its foam-making abilities. The increase in the solids content and foam-making abilities allows to judge about more complete extraction of saponins from the roots Saponaria officanalis L.
The results of experiments on the optimal choice of the extraction conditions are presented in the examples.
Example 1
They took a portion of the roots Saponaria officinalis L. the mass of 6 g, placed in the chemical glass, poured 30 ml of distilled water (water duty 1:5). Leave to soak for 20 minutes. After that conducted ultrasonic treatment was performed using the device IKASONIC U50 control (30 kHz), for which the nozzle of the ultrasonic generator immersed in chemical glass of pre-soaked with raw materials. Ultrasonic action lasted for 20 minutes with intensity 230 W/cm 2 . After extraction, the solution was filtered through a glass filter. The obtained extracts were determined by the content of dry matter and foaming ability.
Under these conditions it foaming ability of the extract was equal 379%, the content of dry substances of 14%.
Example 2
The experience was similar to example 1, except that the process was carried out at the intensity of ultrasonic influence 184 W/cm 2 .
While foaming ability extract was equal to 200%, the content of dry substances of 7.8%
Therefore, the reduction of intensity of ultrasonic influence leads to deterioration of quality of the obtained extracts.
Example 3
The experience was similar to example 1, except that the process was carried out at the intensity of ultrasonic influence 276 W/cm 2 .
While foaming ability of the extract was equal to 43%, the content of dry substances of 8.3%.
Therefore, the increased intensity of ultrasonic influence leads to deterioration of quality of the obtained extracts.
Example 4
The experience was similar to example 1, except that the process was carried out for 15 minutes.
While foaming ability of the extract was equal 346%, the content of dry substances of 13.3%.
Therefore, reduction of time of ultrasonic influence leads to deterioration of quality of the obtained extracts.
Example 5
The experience was similar to example 1, except that the process was carried out for 25 minutes.
While foaming ability of the extract was $ 347%, the content of dry substances of 14%.
Consequently, the increase of time of ultrasonic influence leads to deterioration of quality of the obtained extracts.
These examples show that the optimal conditions for obtaining capodistrias extracts are: ultrasonic treatment intensity 228% to 232 W/cm 2 for 19% to 21 minutes.
The invention is at the stage of laboratory research.
The method of obtaining capodistrias extract, including preliminary soaking roots Saponaria officialis L. in distilled water in the ratio of 1:5 during 19-21 min, extraction under the influence of ultrasound with the frequency of 30 kHz frequency and intensity 228-232 W/cm 2 for 19-21 min at the temperature of 25 C, filtration.