Immunostimulating drug
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FIELD: medicine.
SUBSTANCE: invention refers to an immunostimulating drug on the basis of a herbal alcohol tincture. The above drug is prepared by filling the herbal raw material with 70% ethanol in the ratio of 1:10, keeping in a dark place at temperature 18-20°C for 7 days, filtering, extracting the raw material, and filtering the extract. The herbal raw material contains purple Echinacea herb and blossom clusters, Scotch pine buds, horseheal roots and rhizomes, common licorice roots and harmel herb taken in 1:1:1:1:0.5.
EFFECT: drug possesses the manifested stimulating effect on the immune competent systems that promotes a higher level of the non-specific animal's protection.
1 tbl, 1 ex
The invention relates to veterinary medicine, refers to the means of natural origin and can be used for stimulation factors of immunity and natural resistance in farm animals.
Known drugs with similar action: drugs of biogenic origin, Zymosan (polysaccharides derived from yeast culture); Propolis; Apilak; Ribomunyl (bacterial ribosomes); Bronchosan (lyophilized bacteria); Pasat (combined preparation of autolysates bacteria); Thymalin, Taktivin, Timoptic, Timogen - solutions and lyophilisate for subcutaneous and intramuscular injection, and others; preparations obtained by the method of chemical synthesis Methyluracil, Stronger (stimulants leucopoiesis); Mildronate (similar to carnitine, has an anabolic effect); Arbidol (antiviral agent); Leucatin (pills and solution for injection); Diazepan (solution for injection); Decaris (pill) and others [1]; drugs of plant origin extract eleuterokkoka, aloe, ginseng extract, Immunal (juice of Echinacea purpurea); Tonsillar (combined preparation from plants) [2].
However, for these drugs there are some contraindications, one of which is individual sensitivity, in particular to some immunokorrigiruyuschy is redstem synthetic origin. In addition, the use of funds derived from animal tissue, partially because of the possible presence of prion new forms of pathogens that do not have genome and which are, in fact, infectious proteins that cause fatal slowly progressive degenerative brain diseases. Another limitation is the well-known unpredictability of the actions of some of immunotropic drugs, which, being appointed for one purpose, can lead to undesirable consequences type of autoimmune disorders, allergies or lampropeltis reactions.
Closest to the proposed action is a product of plant origin - Immunal (drops for internal use, consisting of juice, squeezed from freshly blooming plants of Echinacea purpurea (Echinacea purpurea L). However, the effect of this drug is limited, as it contains only one Echinacea purple as the main component.
A new technical challenge is expanding Arsenal of drugs with a higher degree of immunomodulator activity and giving when using fewer complications.
The problem is solved a new immunostimulating agent-based alcohol tinctures obtained from the grass and blossoms of purple coneflower (Echinacea purpurea (L), kidney pine ordinary is vannoy (Pínus sylvéstris), the roots and rhizomes of elecampane {Inula helenium), roots of licorice (Glycyrrhiza glabra L.) and grass Rue ordinary (Peganum harmala), taken in the ratio 1:1:1:1:0,5.
The chemical composition of the proposed medicinal product is presented as a complex of biologically active substances, which ensures its high therapeutic effect [2].
The predominant biologically active substances are: polysaccharides, flavonoids, phenol carbonic acids, triterpene saponins, vitamins, trace elements.
Polysaccharides have immunocorrigirutee, anti-inflammatory and antiviral effects. They stimulate nonspecific immunity and phagocytosis activity of macrophages and neutrophils, as well as bactericidal function of cells. In addition, include specific immune protection, increasing the number of T-lymphocytes, cytokine production, and reduce the activity of hyaluronidase produce bacteria and viruses. The result is stored hyaluronic acid - a substance that is formed in the tissues between cells and serves as a barrier against infections [3].
The flavonoids inherent hepatoprotective properties, which include Polyana holocaustmuseum, anti-inflammatory and capillary components, flavones and balcony possess bacteriostatic property, as well as tor Oset the formation of products of lipid peroxidation (diene conjugates, malondialdehyde, Schiff bases); they have the ability to normalize lipid metabolism in the liver, in particular its phospholipid fraction [4]. Preventing the accumulation of Mesopotamian, show antiphospholipid activity. In addition, flavonoids have antimicrobial, hypolipidemic, membrane stabilizing and antioxidant properties. Included in the collection of the flavonoid quercetin has the property to block the Na+, K+ and mitochondrial (Ca2+)-ATPase and the secretion of histamine mast cells. I believe that the mechanism of action of flavonoids on fat cell is of great importance to their affinity to specific phospholipid components of cell membranes [5].
Tannins (kidney pine up to 5%, Glycyrrhiza glabra - to 9.46%, elecampane - 9,3%), which is dominated by polyphenols have anti-inflammatory, antimicrobial properties and also have antioxidant and antitoxic effect [5].
Anti-inflammatory and anti-allergic properties due to the presence of triterpene saponins: glycyrrhizin (GL) and glycyrrhetinic acid. Ledger on the structure of the aglycone and metabolite glycyrrhetic acid has similarities with the structure of glucocorticoid hormones. The effect of the latter depends on the presence of group - and - uncertain ket is on, oxygen function at C11and Catalinas group in the side chain. The first two element patterns corticoid hormones have glycyrrhetic acid [4].
The high content of ascorbic acid {Echinacea - up to 4.0%), licorice - 3.0% of the buds of Scots pine - 0,3%) stimulates adrenal function, increasing the secretion of cortisol, causing anti-inflammatory and antiallergic effect.
Ions of manganese (manganese content, µg/g: Rue ordinary - 5,8, kidney pine - 88,0) are members of various enzyme systems, affect hematopoiesis, the function of liver cells, the tone of blood vessels, bile ducts, can prevent the formation of intravascular thrombi. Organic salts of iodine (iodine, mcg/g: licorice - 0,09; kidney pine - 0,15) improve protein assimilation, the assimilation of phosphorus, calcium and iron. The trace element zinc (zinc, ug/g; kidney pine - 31,10, licorice is 0.33, Rue ordinary - 0,78, elecampane - 0,27) participates in the body's immune processes, selenium (selenium, ug/g: licorice - 17,1, elecampane - 10,0, Rue ordinary - 1,0, kidney pine - 0,20) is a powerful antioxidant, iron (iron content, mg/g: Echinacea - 0,48, Rue ordinary to 0.14, elecampane is K - 0,9, licorice - 0,7, kidney pine - 0,04) is the main structural component of hemoglobin and hem-comprising enzyme, its salts normalize the body's lipid metabolism and have a stimulatory effect on erythropoiesis. as well as in complex with cobalt (cobalt, ág/g: licorice - 0,03, Rue ordinary - 0,4) has a stimulating effect on the immunogenesis in viral infections, copper (copper content, mg/g: kidney pine - 6.8, elecampane - 1,1, licorice - 0.2, Rue ordinary - 0,37) increases the functional activity of leukocytes, the authors estimated the effect of bactericidal activity of serum.
An example of obtaining the proposed immunostimulating agent.
0.45 kg of air-dry collection is crushed to a particle size of 3 mm, Then the crushed Assembly using a sieve with the hole diameter of 0.25 mm free from dust (particles less than 0.25 mm) and receive 0.45 kg of ready raw materials (consumable coefficient equal to 1.03), which is placed in the vessel of dark glass, pour 4500 ml of 70%ethanol, incubated in the dark at a temperature of 18-20°C for 7 days and filtered through the canvas, pressing the raw material. Immunostimulatory agent applied in the form of 7-8% aqueous solution
Clinical evaluation of immunostimulatory funds held in clinically healthy calves 20-30 days in the Rasta. Was formed two groups of animals 10 animals each (1-experienced introduced immunostimulatory agent in the form of a 7%aqueous solution at a dose of 2.5 ml/kg body weight for 10 days, group 2 was experienced). In the beginning of the experiment conducted screening blood tests for the selection of animals in the experience with the imbalance in the immune system (table), later (after 7 days) tracked changes occurring in the body of calves on the following indicators: functional activity of blood neutrophils, bacteria (BASK) and Lisitsina (FOREPLAY) activity of blood serum, the relative and absolute number of T - and b-lymphocytes, immunoglobulin level.
Background studies (table) revealed that indicators of immune reactivity in animals identified: insufficient level of immunoglobulins G and M, the lowest concentration which shows immunodeficient condition, imbalance in cellular and humoral immunity.
| Table | |
| The effectiveness of an immunostimulating agent for secondary immunodeficiency calves: | |
| Indicators | Groups of animals |
| Experienced | Control |
| T-lymphocytes, % | |
| T-lymphocytes, 109/l | |
| B-lymphocytes, % | |
| B-lymphocytes, 109/l | |
| The functional activity of blood neutrophils, % | |
| spontaneous test | |
| - induced test | |
| Immunoglobulins (mg/ml | |
| - IgG | |
| - | |
| IgM | |
| The authors estimated the effect activity of blood serum, % | |
| Bactericidal activity of blood serum, % | |
| Note: numerator - indicators to experience (background), the denominator is the value after 7 days of experience | |
When comparing parameters BASK in the dynamics of the experience, it was noted that by the end of this experiment show the tel in the experimental calves was significantly higher than in control animals (98,3±2.2% against 80,4±1,9%), TENDERNESS in the experimental group in contrast to the control animals reached lower values of the norm. The analysis of the research data of the cellular and humoral immunity is established that the application of the proposed funds contributed to significant increase in T - and b-lymphocytes. In the experimental group relative number of T cells were 31.0±1.2%, In cells and 8.6±0.4%, while the control animals relative number of T - and b-lymphocytes was 24.2±0,4% and 6.0±0.7%, respectively. In the humoral immunity was determined the content of the classes of immunoglobulins G and M in all the experimental animals. Analysis of these data allowed us to conclude that the tool contributed to significant increase in Ig G (15,0±0.1 mg/ml against 12,8±0.7 mg/ml in control), and the concentration of Ig M a tendency to increase.
The proposed tool containing alcohol tincture medicinal plant collection, is a means of natural origin, made from affordable, environmentally friendly raw materials, and its application has had a pronounced stimulating effect on the immune system of the body, resulting in increased levels of non-specific protection of the animal.
Sources of information
1. D.K. Novikov, Derkach YU.N., But what of Sri P.D. Immunotropic drugs // In the world of drugs. - M., 1999. - 2 (4). - P.48-58.
2. Bakuridze A. D., Kurtsikidze MS, Pisarev V.M. and other Immunomodulators of plant origin (review) // Chem. the pharmacist magazine. - M., 1993. - 8. - S.43-47.
3. Samorodov C. N., Pospelov ST., Moses GF and other Phytochemical composition of the genus Echinacea (Echinacea Moench.) and its pharmacological properties (review) // Chem. the pharmacist magazine. - M., 1996. - 4. - P.32-37.
4. Obolentseva, GV, Litvinenko VI, Ammosov A.S. and other Pharmacological and therapeutic properties of drugs licorice (review) // Chem. the pharmacist magazine. - M., 1999. - 8. - P.24-28.
5. Sokolov, SJ, zamotaev I.P. Handbook of medicinal plants. - M., 1990. - 464 S.
Immunostimulatory agent-based alcohol tinctures of medicinal plant collection, characterized in that as the plant materials used grass and clusters of purple coneflower (Echinacea purpurea L.)buds of Scots pine (Pinus sylvestris), roots and rhizomes of elecampane (Inula helenium), the roots of licorice (Glycyrrhiza glabra L.) and grass Rue ordinary (Peganum harmala), taken in the ratio 1:1:1:1:0,5, vegetable raw material is poured in ethyl alcohol 70%concentration at a ratio of 1:10, incubated in the dark at a temperature of 18-20°C for 7 days, filtered, pressing raw and filtered.