Immunomodulator. RU patent 2497514.

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to chemical-pharmaceutical industry, and represents an immunomodulator for treating chronic hepatitis, hepatic cancer, lymphatic sarcoma, chronic leukemia, and for improving the functions of liver and blood-forming organs, for enhancing the immunobiological body characteristics, prepared by mixing 1000 ml of an aqueous infusion of sandy everlasting blossom, pepper mint herb and chicory herb with 50 ml of bovine serum containing leukaemia oncovirus antibodies, 20 ml of wild rosemary infusion, 40 g of ascorbic acid, 2 g of sorbic acid, 0.2 g of folic acid until the ingredients are dissolved completely, with adding 60 g of liver powder, 30 g of lymphatic node powder, 30 g of young bovine spleen powder; the prepared solution is settled at room temperature for 24 hours, then kept at a boiling water bath for 30 minutes and cooled for 6-8 hours at room temperature; the settled solution is filtered, wherein the aqueous herbal solution is prepared by mixing equal proportions of the separately prepared aqueous infusions of 40 g of pepper mint herb in 1000 ml of water, 30 g of sandy everlasting blossom in 1000 ml of water and 30 g of chicory herb in 1000 ml of water, while the wild rosemary infusion is prepared by infusing 60 g of ground wild rosemary blossom in 1000 ml of 70% purified ethanol.

EFFECT: invention provides creating the high-efficacy agent and reducing the length of treatment.

The invention relates to the field of chemical and pharmaceutical industry, in particular to intended for the treatment of chronic hepatitis, liver cancer, limphosarcoma, chronic leukemia, and improve the functions of the liver and blood forming organs increase of immunobiological properties of the organism. Known «Vitogepat» derived from the liver of cattle, which is a complex of proteins and nukleoproteidov vitamins. The disadvantage of this drug is low therapeutic efficacy, inability to achieve long-term remission and full . The purpose of creation of the invention is to increase medical efficiency and reduction of terms of treatment of the above diseases. This objective is achieved by the immunomodulator additionally contains powder from the lymph nodes and spleen cattle serum of cattle containing antibodies to , Hemlock tincture, aqueous solutions of flowers bessmertnika sand, from grass peppermint, chicory, ascorbic acid, and folic acid in the trail. components:

Powder liver of cattle - 6,0,

Powder lymph nodes to 3.0,

Powder spleen - 3,0,

- Cattle serum containing antibodies to - ml to 5.0

- Hemlock tincture to 2.0 ml

- Flowers of immortelle sandy - 3,0,

- Grass peppermint - 4,0,

- Grass chicory - 3,0,

- Ascorbic acid - 4,0,

- Sorbic acid - 0,2,

- Folic acid, 0,02

- Distilled water up to 100.0 ml

Comparison of the proposed drug with other well-known in the field of medicine and pharmaceutical industry showed its compliance with the criteria of the invention.

Manufacturing of medicinal forms from raw materials of animal origin:

- Liver, lymph nodes(submandibular, , mediastinal) and spleen are selected in young cattle, at the age of 1-1,5 years, without clinical and laboratory manifestations of non-contagious, infectious and parasitic diseases immediately after the slaughter. Bodies are washed with running water and placed in the refrigerator at a temperature of +2+4 C, the 7 day cooling and conservation of biologically active substances. After this time authorities are cleared from the adipose tissue, finely crushed and put in an oven, each authority separately at a temperature of +60 C for 72 hours. Drying cupboard authorities turned over 24 hours since the beginning of drying. In the apparatus is threefold degreasing 96% ethyl alcohol. The vacuum drying Cabinet is removed extractant. Next, the resulting mass separately by the authority is passed through ball mill to powder-like state.

- Manufacturing of serum cattle containing antibodies to , leukemia virus inactivation and preservation of antibodies. At this stage you need to interact with experts of the state veterinary service in rural areas. During routine diagnostic studies cattle on leukemia, selected cows aged 4 to 6 years, positively reacting to leukemia by the reaction of the immune diffusion (RID). Next, the blood of these animals investigate hematological indicators are fine on the number of cells. Selected animals in which the number of white blood cells is 80 thousand and more (1 mm 3 ). This is the period when the disease passes from the blood stage of the tumor. From these animals is taken blood in the volume of 1 litre per head in glass flasks and separated serum. Then, the serum shimmers in 200 ml glass bottles of 100 ml and in them is filled in 100 ml of 70% of the pure ethyl alcohol. The mortar is mixed to obtain a homogeneous mass of white, after which the bottles are closed with rubber stoppers and rolled aluminium caps. Before adding to the product the contents of the vial thoroughly shaken.

Manufacturing of medicinal forms from raw materials of plant origin:

- Medicinal raw material is preliminary crushed with the size of particles passing through a sieve with apertures of 5 mm.

- Preparation infusions of herbs. Infusion of peppermint: in the heated will be 40, herbs, mint and filled with 1000 ml distilled water at room temperature and infused in water bath for 15 minutes with a boiling point with periodic . After that removed, extractor fan cooled at room temperature, same with occasional stirring at least 45 minutes, then filtered through a 4-layer gauze filter.

- Infusion of herbs chicory and flowers bessmertnika sand is prepared similarly, in the ratio of 30:1000.

- Preparation of Hemlock tincture. Tincture is prepared from freshly picked inflorescences of Hemlock in the first half of August. Placed in a flask 60 g of crushed flowers and filled with 1000 ml of 70% of the pure ethyl alcohol infused for 1 month. During this time, the contents periodically mixed. After this time tincture is filtered through a 6-layer gauze filter. The precipitate is discarded. Is stored in the dark place at room temperature.

Technology of preparation of the drug

In 1000 ml infusions flowers bessmertnika sand, grass peppermint and chicory in equal parts added 20 ml of tincture of Hemlock, 50 ml of serum antibodies of after a thorough shaking, 40 g, ascorbic acid, 2, sorbic acid and 0.2, folic acid. The resulting solution is stirred until complete dissolution of the ingredients. In placed 60 g of powder from the liver, 30 g of powder from the lymph nodes, 30 g of powder from the spleen cattle, and filled with 1000 ml of the solution prepared together with sludge. The solution is thoroughly mixed and left for 24 hours at room temperature for swelling, with occasional stirring. After this time is placed in a water bath for 30 minutes with a boiling point, with constant stirring. Then it's removed from the water bath and cooled 6-8 hours at room temperature. To defend the liquid is filtered through a funnel Buechner sediment is discarded. Finished product is packaged in glass bottles of 200 ml and sterilized at a temperature of +100 C for 30 minutes.

Testimony to the use of the drug are:

Chronic hepatitis, liver cancer, lymphoma, chronic leukemia, improvement of the liver and blood-forming organs, improvement of immunobiological properties of the organism. The preparation is applied inside adults and 5 ml of 1 times a day after meal for 20-30 days. In cancer of the liver, lymphosarcoma, chronic leukemia 5 ml, 2 times a day for 30-40 days. Children, depending on age, 1.0 ml to 3.0 ml of 1 time per day for 20-30 days. Repeated courses of treatment can be carried out not earlier than 3 months. Contraindications are acute liver failure, severe coronary insufficiency. The drug belongs to the list B and stored at a temperature of +5, +10oC C. Shelf life of 12 months, is produced in glass bottles with a capacity of 200 ml

The proposed immunomodulator «Hepato- co» is phyto-cytostatic drug, the integrated nature, broad-spectrum, is made exclusively of raw materials of animal and plant origin, which has no elements synthesis of chemical compounds. This medicine acts on the body on the principle of «similarity». When ingestion is rapidly absorbed from the gastrointestinal tract into the blood and has a pronounced anti-inflammatory effect on the liver parenchyma and lymph nodes, causing regeneration inflammatory sites. Components of the drug have antispasmodic and tonic action, reinforce cholagogue and bile secretion, reducing the level of bilirubin, blood cholesterol, prevent the stagnation of bile, improves metabolic function of the liver. Regulate carbohydrate and protein metabolism, thereby significantly improving the functional activity of the liver, increases the nitrogen in the urine. Under the influence of the drug improves the function of blood, that allows to develop full-fledged blood cells and restore the normal transport of oxygen to organs and tissues, improve metabolic processes in the body. Introduction in structure of immunomodulator serum antibodies to due to the fact that the causative agent of bovine leukemia and people is one of the same RNA-containing virus belonging to the family of retroviruses and kind of. This virus is identical affects the hematopoietic system in both people and animals, circulating blood and lymph nodes throughout the body. Cancer cells caused by this virus can in any organs and cause malignant neoplasms. Therefore, this serum passively body and allows to produce its own antibodies to , and ultimately the development of immunity. The drug is generally inhibits the growth and development of cancer cells, prevents metastasis, is a powerful nutrient stimulator and increases immunobiological properties of the organism.

The proposed immunomodulator possible and expedient to use in both inpatient and outpatientclinics during the treatment of diseases such as chronic hepatitis, liver cancer, limphosarcoma, chronic leukemia, and improve the functions of the liver and blood-forming organs, increase of immunobiological properties of the organism.

Sources of information:

- Directory of medicines under the editorship of M. Klyuyev, book house Locus reepol classic «Lada» Moscow 2006, p.154.

- Directory of infectious diseases of animals edited by J.F. ñ . Moscow, IN «» 1987, .152.

- Infectious and parasitic diseases human edited by B.A. Cherkassky. Moscow, publishing house «Medical newspaper», 1989, page 319.

Immunomodulator for the treatment of chronic hepatitis, liver cancer, limphosarcoma, chronic leukemia and improve the functions of the liver and blood-forming organs, increase of immunobiological properties of the organism, obtained by mixing 1000 ml of water infusion of flowers bessmertnika sand, grass peppermint grass chicory with 50 ml of serum cattle containing antibodies to leukemia, 20 ml of tincture of Hemlock, 40 grams of ascorbic, 2 g sorbic, 0.2 g of folic acid before full dissolution of all components, with the subsequent addition of 60 g powder liver, 30 g powder lymph nodes, 30 g powder spleen young cattle, with the further pursuit of the obtained solution at room temperature for 24 hours and then keeping on a boiling water bath during 30 min and cooling for 6-8 hours at a room temperature, and filtering solution, where water infusion of herbs prepare by mixing equal ratio separately received infusions of water 40 g herbs, mint in 1000 ml of water, 30 g flowers bessmertnika sand 1000 ml of the water and 30 g of grass chicory in 1000 ml of water, and Hemlock tincture receive macerating 60 g of crushed inflorescences of Hemlock in 1000 ml of 70% of the pure ethyl alcohol.