Method of normalisation of spontaneous erythrocyte aggregation in piglets with dairy-vegetative nutrition with bronchitis

FIELD: medicine.

SUBSTANCE: invention relates to veterinary, and can be applied for normalisation of spontaneous erythrocyte aggregation (SEA) in piglets with dairy-vegetative nutrition with bronchitis. For this purpose to piglets of diary-vegetative nutrition with bronchitis simultaneously prescribed are crezacine in dose 4 mg/kg per day in scheme of drinking in the morning for five days and gamavit in dose 0.03 mg/kg per day intramuscularly one time per day, for five days.

EFFECT: method makes it possible to normalise level of SEA in piglets, reduce mortality, normalise weight gain, and increase quality of obtained from them in future meat production.

3 ex

 

The invention relates to biology and veterinary medicine, namely to Hematology.

Analogues of the claimed method of normalization of the spontaneous aggregation of erythrocytes (SAE) in piglets dairy and vegetable food with bronchitis does not exist, In the literature there is information about the high biological activity of krezacin - derived amoxilcillin acids, can stimulate various systems of living organisms, increasing their survival under adverse conditions (Baimatov V.N., Latypov M.M. Influence of krezacin on the state of the birds // veterinary medicine. - 2006. No. 11. 49 - 51) and amavita - complex balanced funds on the basis of the placenta, containing nukleinat sodium, set of amino acids, vitamins and salts with high organoprotective and anabolic effect (Belousova W. the Use of amavita to improve reproductive efficiency of swine // veterinary medicine. - 2006. No. 10. - C.11-12). However, in the literature there is no indication about their impact on the UAE and especially on the possibility of normalizing relations with their help, SAE piglets dairy and vegetable food with bronchitis.

The aim of the invention is the normalization of SAE in piglets dairy and vegetable food with bronchitis.

The essence of the proposed method lies in the fact that for the normalization SAE pigs lacto-vegetarian food with bronchitis assigned creatin mg/kg per day, included in the scheme, drink in the morning for five days, and at the same time gamavit 0.03 mg/kg intramuscularly once daily in the morning for five days.

The method enables stable to normalize SAE piglets dairy and vegetable food with bronchitis after 5 days after treatment. In the subsequent data piglets SAE stabilized at a normal level, which can significantly improve the livestock of pigs, to normalize the gain, reduce their pathological burden, reduce mortality and improve the quality received from meat products.

The inventive method is carried out as follows. Erythrocyte aggregation is assessed by the degree of ability of erythrocytes to spontaneously aggregate (Pokrova O.A., Grineva MR, Ivanov K.S. Methodology and clinical significance of the study the rheological properties of blood // Bulletin of the Ivanovo medical Academy. - 2008. - T13, No. 1-2. - s.89-98).

To assess the aggregation of erythrocytes blood from a vein taken in sodium citrate in the ratio of 9:1 and centrifuged 10 minutes at 3000 rpm./minutes In 96 hole taken to fill 2 holes 0.2 ml plasma of the subject. From the tube removed all the plasma and the layer of leukocytes. Erythrocytes resuspendiruetsa standard phosphate buffer in the ratio 1:4, followed by centrifugation for 10 minutes at 3000 rpm./minutes, allowing you to wash the x residues from plasma when removing the supernatant. After this is taken as 0.02 ml of erythrocytes and resuspended first filled with autologous plasma hole hole 96 Ouija boards, allowing you to get 10% hematocrit. Then from this hole, take a clean, dry pipette 0,02 ml content and placed in the second filled the hole that allows you to get 1% hematocrit. After that 1 grid in the camera Goryaeva fill obtained a suspension of red blood cells, incubated for 3 min for the occurrence of spontaneous aggregation and shall count free erythrocytes (including 2 of the erythrocyte together) and units, starting with 3 cells, connected in the form of "monetary columns") in 2 large squares of the camera (lens × 40, eyepiece × 10). Considered the number of "coin of columns and the number of red blood cells, involved in them.

Based on these results, calculate the following indicators that assess SAE:

1) Average unit size (CPA):

CPA=sea/KA

Sea - the sum of all cells in the units;

KA - the number of units

2) the Index of aggregation (PA):

PA=(CPA·KA+CTU)/(KA+CTU)

CTU - free red blood cells

3) the Percentage of non-aggregated RBCs (PNA):

PNA=CSE·100/(CPA·KA+CTU)

Normative values for CPA are 4-5, PA 1,05-1,30, for the PNA 80,0-92,0%. The increase in CPA more than 5 PA above 1.30 and lowering the PNA below 80,0% talks about increasing the SAE. In the case of violations the Oia at least one of these three indicators of sick animals must be treated according to the inventive method, including krezacin 4 mg/kg per day in the schema drink for 5 days and at the same time gamavit of 0.03 ml/kg intramuscularly once a day for five days.

The use of the claimed complex normalizes SAE piglets dairy and vegetable food with bronchitis after 5 days after treatment. This is the basis of recovery of the herd, reducing mortality and guarantee of high quality meat products from treated with it, pigs.

Example 1. A pig, dairy and vegetable nutrition No. 28 bronchitis was found increased SAE (CPA=5,8, PA=1,86, PNA=63,4%). The pig were appointed at the same time krezacin 4 mg/kg per day in the schema drink in the morning for 5 days and gamavit of 0.03 ml/kg intramuscularly once daily in the morning for five days.

Through 5 days after the end of treatment for pig registered full normalization SAE (CPA=4,2, PA=1,19, PNA=84,5%). In later achieved her performance in the pig did not experience negative dynamics.

Example 2. A pig, dairy and vegetable nutrition No. 31 with bronchitis was found increased SAE (CPA=5,5, PA=1,79, PNA=65,8%). The pig were appointed at the same time krezacin 4 mg/kg per day in the schema drink in the morning for 5 days and gamavit of 0.03 ml/kg intramuscularly once daily in the morning for 5 days.

Through 5 days after the end of treatment on osenka achieved full normalization level SAE (CPA-4,5, PA=1,21, PNA=82,4%). Subsequent observation carried indices in this pig did not experience negative dynamics.

Example 3. A pig, dairy and vegetable nutrition No. 34 with bronchitis was found increased SAE (CPA=5,7, PA=1,86, PNA=69,3%). The pig were assigned krezacin 4 mg/kg per day in the schema drink in the morning for 5 days and gamavit of 0.03 ml/kg intramuscularly once daily in the morning for 5 days.

Through 5 days after the end of treatment the pig reached full normalization SAE (CPA=4,3, PA=1,27, PNA=88,4%), in the following not experienced negative dynamics.

The use of the claimed method has been successfully tested on 174 pigs dairy and vegetable nutrition bronchitis with achievement of full normalization SAE in all cases.

The method of normalization of the spontaneous aggregation of red blood cells in piglets dairy and vegetable food with bronchitis, including simultaneous application of krezacin 4 mg/kg per day in the schema drink in the morning for five days and amavita of 0.03 ml/kg intramuscularly once daily in the morning for five days.



 

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