Method for preventing and treating liver dystrophy in cattle. RU patent 2494759.

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention relates to therapy of internal non-infectious diseases, particularly to compositions for preventing and treating liver dystrophy in cattle. An agent for preventing and treating liver dystrophy in cattle contains an antihepatotoxic serum and an antispleenotoxic serum; the serums are pre-processed in acidic and enzymatic hydrolysis at pH 3.9-4.0 and pepsin concentration 49-51 mg per protein 100 g for 17.5-18.5 hours at temperature 37-38°C; then hydrolised serums are mixed with sterile saline, preserved phenol concentrated 0.4-0.5% at 1.0 ml thereof, the antihepatotoxic serum - 9.0-11.0 titre units and the antispleenotoxic serum - 9.0-11.0 titre units.

EFFECT: invention enables more effective prevention and treatment of the liver dystrophy in cattle, higher production rate.

1 tbl, 1 ex

The invention refers to the veterinary medicine and can be used for the prevention and treatment of malnutrition in the liver of cattle.

Known treatment and prevention of liver dystrophy through the introduction in a diet of . After application of hepatoprotector in animals improves clinical status, normal level of biochemical parameters and functional status of the liver. (5)

The use of this tool requires daily villas him with food, which significantly increases the cost of the diet.

Known for prevention and treatment of liver dystrophy in cows by daily administration of 200 mg cocarboxylase and 5 ml of sodium salt acid.

In this scheme of liver function of all experimental cows. This increases the amount of glucose in the blood and decreases the amount of acetone, acetoacetate and beta-hydroxybutyric acid. Free fatty acids are esterification and included in the metabolic processes. (4)

Disadvantage application of these drugs is their high cost and the necessity of daily administration.

Known complex of means for the prevention and treatment of hepatosis in cows. He is the application and cocarboxylase intramuscularly, ascorbic acid subcutaneously, mixture B intraperitoneally on propisi and , artificial carlsbad salt and mineral supplements inside during the month.

After the treatment, the number of total lipids in blood serum increased to 451,87±12,66 mg %, which is 1 1,14% above the initial data, the content of free and cholesterol to 26.5±1.7 and 207,85±11,47 mg %, which 53,09 and 25,09% more than the initial data. These changes indicate the normalization of liver function in cows. (3)

The disadvantage of this complex into large number of them, and difficulties intraperitoneal injection.

Known complex of means of prevention, which consists in the introduction in a diet of diammonium phosphate in the dose of 60 to 80 grams per day and intramuscular but 15 ml, 2 times a month. (2)

The disadvantage of this complex is the presence of diammonium phosphate, which does not allow to balance a diet of phosphorus and causes complication hepatosis.

Known tool for the prevention and treatment of liver dystrophy CSD, based on the use of serum. The use of small doses of whey (0,2 units) once to normalize violated as a result of the pathological process functional state of the liver.(1)

The advantage of this tool is single use and low cost.

This tool closest to the technical nature and chosen for the prototype has the following disadvantage: to complete the work serum need to determine the immune status of livestock and adjust the dose serum.

The aim of the invention is a means of prevention and treatment hepatosis in cattle, allowing to prevent diseases of the liver and stimulate the bodies immune system. This goal is achieved by the fact that in addition to serum is added serum, which enhances the action of serum and increases the natural resistance of the organism, which is very important when hepatosis. and serum pre-exposed to the acid- hydrolysis. The drug is referred to herein as «» () for cattle.

The technical result of the invention is to strengthen the action of the product «» for cattle compared with serum and increase the natural resistance of the organism when .

The product is injected cattle at a dose of 1.0 ml of 50 kg body weight, subcutaneously or intramuscularly.

The preparation is a transparent liquid with insignificant sediment, easy when shaken. Shelf life when stored in a temperature of +5 - +7 OC - 2 years.

The proposed scheme of obtaining the drug «» for cattle consists of the following stages:

1. Getting the antigen from the liver. For the preparation of the antigen from the liver of use liver healthy cattle. The pieces 4-6 times were washed off the blood 10-fold volume of isotonic solution of sodium chloride. Pieces-shredded, pounded in a mortar with glass and throw 0,85% solution of sodium chloride 10%concentration. The resulting suspension was centrifuged 9-10 minutes at 2500-3000 rpm and as antigen use liquid. Antigen fined five times the freezing and thawing followed by centrifugation at 7000-8000 rpm for 10 minutes. Store antigen in a frozen state.

2. Getting the antigen from the spleen. For the preparation of the antigen from the spleen use spleen healthy cattle. Pieces spleen 4-6 times were washed off the blood 10-fold volume of isotonic sodium chloride solution. Pieces-shredded, pounded in a mortar with glass and throw 0,85% solution of sodium chloride to 1 0%concentration. The resulting suspension was centrifuged 9-10 minutes at 2500-3000 rpm and as antigen use liquid. Antigen fined five times the freezing and thawing followed by centrifugation at 7000-8000 rpm for 9-10 minutes. Store antigen in a frozen state.

3. The choice of donor animals. For the preparation of «» as donors suitable following animals: horses, rabbits, cattle and small cattle. Donors should be free from infectious diseases and pass the quarantine.

4. Getting serum. - serum receive by immunization with according to the scheme: the first introduction of antigen conducted subcutaneously in the area of the left shoulder blade and the right of the popliteal fossa; second introduction - after 6-8 days subcutaneously in the area of the right shoulder and the left popliteal fossa; third introduction of a through the same term as second intravenously and subcutaneously in the above listed above point. After 6-8 days after the third injection antigen do a blood test and define the title obtained serum in the reaction of binding complement. If there titre of at least 1:160-1:200 conduct blood sample. Collected blood is placed in a thermostat on 29-30 minutes at 37-38 C and then in sterile conditions separate the clot from the walls and leave the tube in the refrigerator for better retraction of the clot until the next day. The resulting serum sucked off and canned phenol to 0.5%concentration of substances in the serum.

5. Getting serum. whey is produced by donors according to the scheme: the first introduction of antigen conducted subcutaneously in the area of the left scapula and right popliteal fossa; second introduction - after 6-8 days subcutaneously in the area of the right shoulder and the left popliteal fossa; third introduction of a through the same term as second intravenously and subcutaneously into the above point. After 6-8 days after the third injection antigen do a blood test and define the title obtained serum in the reaction of binding complement. If there titre of at least 1:160-1:200 conduct blood sample. Collected blood is placed in a thermostat on 29-30 minutes at 37-38 C and then in sterile conditions separate the clot from the walls and leave in the refrigerator for better retraction of the clot until the next day. The resulting serum sucked off and canned phenol to 0.5%concentration of substances in the serum.

6. Acid is an enzyme hydrolysis sera (pH 3.9 to 4.0 (with set 0.1 n hydrochloric acid) and concentration of pepsin 49-51 mg per 100 grams of protein conducted over 17,5 to 18.5 hours at a temperature of 37-38°C. Hydrolysis of each serum conducted separately. The adsorption of the enzyme aluminium hydroxide (10-20 ml of ammonium gel per 1 liter of serum content of Al 2 O 3 1,2-1,5 g per 100 ml) at pH 4,0-4,5 and a temperature of 20-25°C conducted over 59-61 minutes. Complex aluminium hydroxide - calcium removed clarifying filtration.

7. Getting the drug «» for cattle. To obtain the drug «» for cattle hydrolyzed and serum is mixed with sterile saline, canned phenol to 0.5%concentration with so that in 1.0 ml him was contained by a 9.0-11.0 in units and sera by the reaction of binding complement.

A comparative analysis of the claimed preparation tool chosen for the prototype showed that «» for cattle has the following distinctive sign:

- additionally includes , which as serum was subjected to pre-acid- hydrolysis.

The presence of a distinguished from the prototype characteristic provides the compliance of the proposed technical solution criterion of «novelty».

Tests of «» for cattle confirm its advantages over drug prototype.

A sample run. Getting «» for cattle.

1. Getting the antigen from the liver. For the preparation of the antigen from the liver used the liver healthy cattle. The pieces 5 times washed the blood from 10-fold volume of isotonic sodium chloride solution. Pieces of crushed, ground in a mortar with glass and bred 0,85% solution of sodium chloride 10%concentration. The resulting suspension was centrifuged for 10 minutes at 3000 rpm and as antigen used liquid. Antigen five freezing and thawing followed by centrifugation at 8000 rpm for 10 minutes. Kept antigen in a frozen state.

2. Getting the antigen from the spleen. For the preparation of the antigen from the spleen used spleen healthy cattle. Pieces spleen 5 times washed the blood from 10-fold volume of isotonic sodium chloride solution. Pieces of crushed, ground in a mortar with glass and bred 0,85% solution of sodium chloride 10%concentration. The resulting suspension was centrifuged for 10 minutes at 3000 rpm and as antigen used liquid. Antigen five freezing and thawing followed by centrifugation at 8000 rpm for 10 minutes. Kept antigen in a frozen state.

3. As a donor used the healthy animals, quarantine the past, in particular, healthy rabbits.

4. Getting serum. serum was obtained by immunization with donors according to the scheme: the first introduction of antigen conducted subcutaneously in the area of the left shoulder blade and the right of the popliteal fossa in the dose of 40 mg of protein; second introduction - after 7 days - subcutaneously in the area of the right the shovels, and the left popliteal fossa in the dose of 60 mg protein; a third was performed at the same time as their second intravenously at a dose of 80 mg protein and subcutaneously at a dose of 50 mg in the above point. After 7 days after the third injection antigen trial did blood work and determined the title obtained serum in the reaction of binding complement. If there titre of at least 1:160-1:200 conducted a blood sample from a rabbit in the amount of 50 ml Received the blood was placed in a thermostat at 30 minutes at 37 C and then in sterile conditions separated the clot from the walls of the tubes and left the tube of blood in the refrigerator for better retraction of the clot until the next day. The resulting serum pumped out and canned phenol to 0.5%concentration of substances in the serum.

5. Getting serum. - serum was obtained by immunization with donors according to the scheme: the first introduction of antigen conducted subcutaneously in the area of the left the shovels, and the right of the popliteal fossa in the dose of 40 mg of protein; second introduction - after 7 days - subcutaneously in the area of the right shoulder and the left popliteal fossa in the dose of 60 mg protein; a third was performed at the same time as their second intravenously at a dose of 80 mg protein and subcutaneously at a dose of 50 mg in the the above point. After 7 days after the third injection antigen trial did blood work and determined the title obtained serum in the reaction of binding complement. If there titre of at least 1:160-1:200 conducted a blood sample from a rabbit in the amount of 50 ml Received the blood was placed in a thermostat at 30 minutes at 37 C, then in sterile conditions separated the clot from the walls of the tubes and left the tube of blood in the refrigerator for better retraction of the clot until the next day. The resulting serum pumped out and canned phenol to 0.5%concentration of substances in the serum.

6. Acid is an enzyme hydrolysis sera (pH 3.9 to 4.0 (with set 0.1 n hydrochloric acid) and concentration of pepsin 50 mg per 100 grams of protein held for 18 hours at a temperature of 37 deg C. Hydrolysis of each serum conducted separately. The adsorption of the enzyme aluminium hydroxide (10-20 ml of ammonium gel per 1 liter of serum content of Al 2 O 3 1.3 g in 100 ml) at pH 4,3 and a temperature of 25 degrees C was performed within 1 hour. Complex aluminium hydroxide - calcium removed clarifying filtration.

7. Getting the drug «» for cattle. To obtain the drug «» for cattle hydrolyzed and serum is mixed with sterile saline, canned (phenol to 0.5%concentration with so that in 1.0 ml of its contained on 10,0 units and sera but the reaction of binding complement.

Comparison of efficiency of actions «» but relative to serum conducted in the experience of the bulls by the age of 4 months and average live weight of 150 kg

With this purpose, 20 Bychkov divided on the principle of steam-analogs in the two groups of 10 animals each group. All the animals was in the same conditions of feeding and maintenance.

Animals of the first group served as the control, they have introduced serum in a dose of 1 ml PA 50 kg with the content serum 10,0 units in 1 ml

Animals of the second group were injected «» in a dose of 1 ml per 50 kg of live weight.

Observation of animals was performed within 1 month.

Determination of content of total protein and protein fractions in blood serum is the most simple way of evaluating the actions of the «» on the liver. The results of the research are reflected in the table.

The findings of experience are reflected in table 1.

1. The results of the biochemical analysis of blood serum of hens with the use of «» and serum

Indicator

Group Bychkov

Norm

1 group

2 group

Total protein, g/l

72-86

81,72±1,20

82.30 level±1,48

Albumins, %

30-50

PCs 39,90±0,95

Alpha-globulins, %

12-20

7,22±0,55

8,00±0,78

Beta globulins, %

10-16

11.72 points to±0.19

9,40±0,67*

Gamma-globulins, %

25-40

24,25±0,74

21,45±0,29*

Ratio A/G

0,4-1,0

0,6

0,7

* - p<0.05

These changes show the strengthening of protein-synthesizing function of the liver, increasing its metabolic activity, reducing the risk of hepatosis and increase the natural resistance of the organism.

«» for cattle introduced cattle in a dose of 1 ml per 50 kg of weight body subcutaneously or intramuscularly.

«» for cattle is a transparent liquid with insignificant sediment, easy when shaken. Shelf life when stored in a temperature of +5 - +7 OC 2 years.

List of literature

1. Action specific serums on the sex glands / Y. , N.V. , L.I. Barchenko, O.V. , T.M. Zelenskaya, A.G. . - K.: Naukova Dumka, 1977. - 216 C.

2. Diagnosis and prevention of liver disease in cows. / A.T. labzina, M.A. , STAMP Artamonova // Prevention and eradication of diseases of domestic animals and birds / Ulyanovsk agricultural Institute.- Ulyanovsk. - 1977. - Pp.33-39.

3. Change of lipid metabolism in the blood serum of highly productive cows at hepatosis. / Postnikov V.S., NC // Questions of veterinary biology: Sat. nauch. Trudy inst. - Moscow, 1988. - P.22-23.

4. Warning liver disease in cows. / M. Emelyanov, A.D. Shusharin, L.I. Novoselova, L.A. , V.G. // Etiology and prevention of animal diseases: Proceedings of the Sverdlovsk and Kirov agricultural institutions, including 56. - Perm, 1976. - P.3-6.

5. , I.PH. Diagnosis and treatment of hepatosis in cattle in the areas of ecological trouble / I.PH. Actual problems of veterinary medicine, animal husbandry, social studies and training in the southern Urals: Materials of the scientific, scientific and methodological and methodical conference (28-29.02.1996 and 1.03.1996, Chelyabinsk) / Chelyabinsk. - 1996. - .68-70.

The remedy for the prevention and treatment of malnutrition the liver of cattle, including serum, characterized in that it additionally contains whey and whey subjected to a pre-acid- hydrolysis (pH 3.9 to 4.0 and concentration of pepsin 49-51 mg per 100 grams of protein in during 17,5 to 18.5 hours at a temperature of 37-38°C), then hydrolyzed whey mixed with sterile saline, canned phenol to 0.4-0.5 percent concentration, the rate of detention in 1.0 ml him, serum - 9,0-11,0 units and serum - 9,0-11,0 units.